CN110124764A - Reagent evaporation retardation device and its suppressing method for fully automatic liquid processing - Google Patents
Reagent evaporation retardation device and its suppressing method for fully automatic liquid processing Download PDFInfo
- Publication number
- CN110124764A CN110124764A CN201910462886.4A CN201910462886A CN110124764A CN 110124764 A CN110124764 A CN 110124764A CN 201910462886 A CN201910462886 A CN 201910462886A CN 110124764 A CN110124764 A CN 110124764A
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- China
- Prior art keywords
- cover board
- silica gel
- fully automatic
- automatic liquid
- liquid processing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 239000007788 liquid Substances 0.000 title claims abstract description 72
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 40
- 238000012545 processing Methods 0.000 title claims abstract description 40
- 230000008020 evaporation Effects 0.000 title claims abstract description 36
- 238000001704 evaporation Methods 0.000 title claims abstract description 36
- 238000000034 method Methods 0.000 title claims abstract description 23
- 239000000741 silica gel Substances 0.000 claims abstract description 38
- 229910002027 silica gel Inorganic materials 0.000 claims abstract description 38
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 37
- 238000007789 sealing Methods 0.000 claims abstract description 37
- 239000000463 material Substances 0.000 claims abstract description 20
- 239000002904 solvent Substances 0.000 claims abstract description 11
- 239000012530 fluid Substances 0.000 claims abstract description 7
- 238000012546 transfer Methods 0.000 claims description 8
- 229910003460 diamond Inorganic materials 0.000 claims description 5
- 239000010432 diamond Substances 0.000 claims description 5
- 239000004744 fabric Substances 0.000 claims 1
- 230000005764 inhibitory process Effects 0.000 claims 1
- 238000012360 testing method Methods 0.000 abstract description 4
- 230000007246 mechanism Effects 0.000 abstract description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 12
- 230000008569 process Effects 0.000 description 10
- 238000005516 engineering process Methods 0.000 description 4
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000003292 glue Substances 0.000 description 2
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 229910052710 silicon Inorganic materials 0.000 description 2
- 239000010703 silicon Substances 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 1
- 239000005030 aluminium foil Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 235000019253 formic acid Nutrition 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 238000004801 process automation Methods 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
- B01L3/0213—Accessories for glass pipettes; Gun-type pipettes, e.g. safety devices, pumps
Landscapes
- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention proposes the reagent evaporation retardation devices and its suppressing method that handle for fully automatic liquid, uniformly distributed several reservoirs on orifice plate, reservoir is for placing easy volatile solvent, it compresses and connects with cover board above orifice plate, so that orifice plate and cover plate for sealing, uniformly distributed several silica gel sealing heads corresponding with reservoir on cover board, silica gel sealing head is the trench structure of opening upwards, the bottom of each silica gel sealing head is provided with gap, the material of cover board is self-closing silica gel material, cover board is an integral structure, its suppressing method is specially prior to covering cover board on orifice plate, all fluid treatment operations puncture cover board by liquid-transfering gun and realize.Reagent evaporation retardation device and its suppressing method of the present invention for fully automatic liquid processing, can effectively inhibit the volatilization of volatile liquid, simplify the complexity of fully automatic liquid processing mechanism, and guarantee the accuracy of testing result.
Description
Technical field
The invention belongs to fully automatic liquid processing technology field, the reagent for being especially for fully automatic liquid processing is waved
Hair inhibits device.
Background technique
With the development of automatic technology, before replacing manual operations to carry out sample using fully automatic liquid processing workstation
Reason is gradually by the approval of people.In contrast to manual operations, fully automatic liquid processing workstation have high throughput, high reliability,
The features such as full-automatic, while the operation of organic solvent and high intensity can effectively be avoided to endanger to experimenter's bring body
Evil.For the difference of sample storage carrier, full-automatic sample liquid processing workstation needs are adjusted correspondingly, the sample of mainstream
Product processing carrier has centrifuge tube, microwell plate etc..
During sample process, reagent often has strong volatility, and can the volatilization problems that solve reagent will determine
The accuracy of testing result and the automatization level of pretreatment process.How to inhibit reagent the volatilization for the treatment of process be automation
An important ring for equipment.For centrifuge tube, it can be realized by the method for adding spiral cover in automation equipment and inhibit reagent volatilization
Purpose；And microwell plate then passes through the volatilization in covering heat-sealing film reduction handling process.Existing automation equipment can be using integrated
Automatic film sealing machine plastics sealing plate film or aluminium foil are sealed on microwell plate, achieve the purpose that reduce reagent volatilization.This mode master
There is the shortcomings that three aspects: one, cannot effectively inhibit the reagent volatilization during liquid feeding.Which during liquid feeding not yet
Sealer, has formic acid, methanol, acetonitrile etc. the liquid of high volatile, and the secondary ordered pair testing result of liquid feeding has had an impact；
Two, it can not achieve the evaporation retardation for turning liquid process.Since heat-sealing film is to contact realization with the external world by obstructing the opening of microwell plate
Evaporation retardation, automatic liquid shifting equipment needs to pierce heat-sealing film and works during turning liquid, since heat-sealing film is disposable consumption
Material turns liquid heat-sealing film damaged later in failure state, is needing the occasion which for repeatedly turning liquid that cannot play very well
Evaporation retardation effect；Three, film-covering mechanism dramatically increases working space, and makes the device is complicated to change.After addition automatic film sealing machine
Also to cooperate automatic loading and unloading mechanism, just be avoided that manual operation, realize pretreatment process automation.
Summary of the invention
In view of this, the present invention is directed to propose for the reagent evaporation retardation device that fully automatic liquid is handled, to provide one
Kind structure is simple and convenient to operate, and can effectively inhibit the reagent evaporation retardation device of the solvent volatilization during liquid feeding.
In order to achieve the above objectives, the technical scheme of the present invention is realized as follows:
For the reagent evaporation retardation device of fully automatic liquid processing, including cover board and orifice plate, uniformly distributed several storages on orifice plate
Liquid bath, reservoir are compressed with cover board above orifice plate and are connected, so that orifice plate and cover plate for sealing, cover board for placing easy volatile solvent
Upper uniformly distributed several silica gel sealing heads corresponding with reservoir, silica gel sealing head are the trench structure of opening upwards, each silica gel
The bottom of seal head is equipped with gap, and the material of cover board is self-closing silica gel material, and cover board is an integral structure.
Further, the material of cover board is self-closing functional material, and the material of cover board is rubber.
Further, gap is in cross or linear type.
Further, the thickness range of silica gel sealing head is 1mm-4mm, can effectively realize reservoir week with orifice plate compression
To sealing.
Further, the outer wall of silica gel sealing head is bonded with the inner wall of orifice plate reservoir.
Further, the cross section of cover board is identical with orifice plate cross-sectional structure, is rectangle, circle, rectangular or diamond shape.
Further, self-closing silica gel material is specially SS-6139.
Further, each silica gel sealing head is located above a reservoir, and the upper surface of silica gel sealing head and cover board
The distance range at top is 1mm-1.5mm.
Further, the trench structure of silica gel sealing head is circular trough, square groove, rectangular channel or diamond shape slot.
Compared with the existing technology, it is of the present invention for fully automatic liquid processing reagent evaporation retardation device have with
Lower advantage:
(1) the reagent evaporation retardation device of the present invention for fully automatic liquid processing, the silicon with miniature gap
Glue cover board, while liquid transfer gun head being allowed to puncture, the solvent volatilization being able to suppress during liquid feeding, while guaranteeing that process detects below
The accuracy of data.
(2) the reagent evaporation retardation device of the present invention for fully automatic liquid processing, structure is simple, occupied space
It is small, it is easy to use.
(3) the reagent evaporation retardation device of the present invention for fully automatic liquid processing, first covers silica gel plate, carries out again
The method of fluid treatment operation (including but not limited to liquid feeding, liquid relief, incubation, mixing etc.), has fundamentally overturned existing routine
The operating process of liquid handling work station can effectively locate so that fully automatic liquid processing workstation is more widely applied
Manage volatile solvent.
(4) the reagent evaporation retardation device of the present invention for fully automatic liquid processing, the material of cover board are self-closing
Silica gel is closed, the material of cover board neither reacts with solvent, and has the function of elastic recovery to gap.
Another object of the present invention is to propose a kind of suppression of reagent evaporation retardation device for fully automatic liquid processing
Method processed, it is a kind of easy to operate to provide, it can effectively inhibit the method that solvent volatilizees in Liquid-treatment processes.
In order to achieve the above objectives, the technical scheme of the present invention is realized as follows:
A kind of suppressing method of the reagent evaporation retardation device for fully automatic liquid processing, including specific steps are as follows:
Before being loaded into automatic fluid processing equipment, cover board is covered on orifice plate；
All fluid treatment operations puncture cover board by liquid transfer gun head and realize.
The suppressing method of the reagent evaporation retardation device for fully automatic liquid processing is used for Full Automatic Liquid with above-mentioned
Possessed advantage is identical compared with the existing technology for the reagent evaporation retardation device of body processing, and details are not described herein.
Detailed description of the invention
The attached drawing for constituting a part of the invention is used to provide further understanding of the present invention, schematic reality of the invention
It applies example and its explanation is used to explain the present invention, do not constitute improper limitations of the present invention.In the accompanying drawings:
Fig. 1 is the schematic diagram of fully automatic liquid treatment process described in the embodiment of the present invention；
Fig. 2 is the structural representation described in the embodiment of the present invention for the reagent evaporation retardation device of fully automatic liquid processing
Figure；
Fig. 3 is the top view described in the embodiment of the present invention for the reagent evaporation retardation device of fully automatic liquid processing；
Fig. 4 is the side view described in the embodiment of the present invention for the reagent evaporation retardation device of fully automatic liquid processing；
Fig. 5 is the cross-sectional view described in the embodiment of the present invention for the reagent evaporation retardation device of fully automatic liquid processing；
Fig. 6 is the side view of cover board described in the embodiment of the present invention；
Fig. 7 is the suppressing method described in the embodiment of the present invention for the reagent evaporation retardation device of fully automatic liquid processing
Flow chart.
Description of symbols:
1- cover board；11- silica gel sealing head；12- gap；2- orifice plate；21- reservoir；3- liquid transfer gun head；4- automates liquid relief
System.
Specific embodiment
It should be noted that in the absence of conflict, the feature in embodiment and embodiment in the present invention can phase
Mutually combination.
In the description of the present invention, it is to be understood that, term " center ", " longitudinal direction ", " transverse direction ", "upper", "lower",
The orientation or positional relationship of the instructions such as "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outside" is
It is based on the orientation or positional relationship shown in the drawings, is merely for convenience of description of the present invention and simplification of the description, rather than instruction or dark
Show that signified device or element must have a particular orientation, be constructed and operated in a specific orientation, therefore should not be understood as pair
Limitation of the invention.In addition, term " first ", " second " etc. are used for description purposes only, it is not understood to indicate or imply phase
To importance or implicitly indicate the quantity of indicated technical characteristic.The feature for defining " first ", " second " etc. as a result, can
To explicitly or implicitly include one or more of the features.In the description of the present invention, unless otherwise indicated, " multiple "
It is meant that two or more.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " installation ", " phase
Even ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or be integrally connected；It can
To be mechanical connection, it is also possible to be electrically connected；It can be directly connected, can also can be indirectly connected through an intermediary
Connection inside two elements.For the ordinary skill in the art, above-mentioned term can be understood by concrete condition
Concrete meaning in the present invention.
The present invention will be described in detail below with reference to the accompanying drawings and embodiments.
For the reagent evaporation retardation device of fully automatic liquid processing, as shown in Figures 1 to 7, including cover board 1 and orifice plate 2,
Uniformly distributed several reservoirs 21 on orifice plate 2, for placing easy volatile solvent, 2 top of orifice plate is compressed with cover board 1 to be connected reservoir 21,
So that orifice plate 2 and cover board 1 seal, uniformly distributed several silica gel sealing heads 11 corresponding with reservoir 21, silica gel sealing head on cover board 1
11 be the trench structure of opening upwards, and the bottom of each silica gel sealing head 11 is equipped with gap 12, and the material of cover board 1 is self-closing silicon
Glue, the material of cover board 1 should ensure not react with solvent, and have the function of elastic recovery to gap 12, and cover board 1 is integrated
The airtightness of cover board 1 is effectively ensured in formula structure.
Further, the material of cover board is self-closing rubber.
Gap 12 is cross gap, and the width of gap 12 is less than in 0.1mm, and length is less than 2mm, to guarantee to automate
Liquor-transferring system 4 drives liquid transfer gun head 3 to enter and extract the stability of cover board 1, and liquid transfer gun head 3 is logical in gap 12 for that can enter
With type standard pipette tips.
1 edge of cover board is retracted 1mm than 2 edge of orifice plate, to guarantee not generating interference during automated handling.
11 outer wall of seal head of cover board 1 is bonded with 21 inner wall of reservoir of orifice plate 2, easy to disassemble.
The cross section of cover board 1 is identical with 2 cross-sectional structure of orifice plate, is rectangle, circle, rectangular or diamond shape, according to using
Environment is different and selects suitable shape.
Self-closing silica gel material is specially SS-6139, through test in normal state due to silica gel naturally stretch it is micro-
Type gap can effectively limit the volatile quantity of liquid, and by taking methanol as an example, when using self-closing silica gel cover board 1, methanol is average per small
When volatile quantity be lower than 4 microlitres, when without covering plate 1, the average volatile quantity per hour of methanol is up to 90 microlitres, which can be effective
Inhibit solvent volatilization.
Each silica gel sealing head 11 is located at 21 top of a reservoir, and the upper surface of silica gel sealing head 11 and cover board 1 push up
The distance range in portion is 1mm-1.5mm, on the one hand convenient for users to identification, on the other hand reduces liquid transfer gun head 3 and needs across lid
The thickness of plate 1 guarantees the stability of work.
The trench structure of silica gel sealing head 11 is circular trough.
The thickness range of silica gel sealing head 11 is 1mm-4mm, and 21 circumferential direction of reservoir can effectively be realized by compressing with orifice plate 2
Sealing.
The trench structure of silica gel sealing head 11 is circular trough, inside diameter ranges 6mm-8mm.
A kind of course of work of the reagent evaporation retardation device for fully automatic liquid processing are as follows:
Before being loaded into automatic fluid processing equipment, cover board 1 is covered in advance above orifice plate 2, and compress；To liquid from
In dynamicization treatment process, cover board 1 maintains closed state；The operation such as liquid feeding and liquid relief drives liquid relief by automation liquor-transferring system 4
Pipette tips 3 puncture the gap 12 on cover board 1 to realize；Gap 12 has function of self closing, and being able to maintain the speed that evaporates is allowing model
In enclosing, automation liquor-transferring system 4 is HAMILTON company liquid relief module, model ZEUS-800741, liquid transfer gun head 3 be and oneself
The matched universal pipette tips of dynamicization liquor-transferring system 4, specific structure is the prior art, and details are not described herein.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention.It is all in essence of the invention
Within mind and principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (10)
1. the reagent evaporation retardation device for fully automatic liquid processing, it is characterised in that: equal on orifice plate including cover board and orifice plate
Several reservoirs of cloth, reservoir are compressed with cover board above orifice plate and are connected, so that orifice plate is close with cover board for placing easy volatile solvent
It seals, uniformly distributed several silica gel sealing heads corresponding with reservoir on cover board, silica gel sealing head is the trench structure of opening upwards, often
The bottom of a silica gel sealing head is equipped with gap, and the material of cover board is self-closing silica gel, and cover board is an integral structure.
2. the reagent evaporation retardation device according to claim 1 for fully automatic liquid processing, it is characterised in that: cover board
Material be self-closing functional material, the material of cover board is rubber.
3. the reagent evaporation retardation device according to claim 1 for fully automatic liquid processing, it is characterised in that: gap
In cross or linear type.
4. the reagent evaporation retardation device according to claim 1 for fully automatic liquid processing, it is characterised in that: silica gel
The thickness range of seal head is 1mm-4mm, and the sealing that can effectively realize reservoir circumferential direction is compressed with orifice plate.
5. the reagent evaporation retardation device according to claim 1 for fully automatic liquid processing, it is characterised in that: silica gel
The outer wall of seal head is bonded with the inner wall of orifice plate reservoir.
6. the reagent evaporation retardation device according to claim 1 for fully automatic liquid processing, it is characterised in that: cover board
Cross section it is identical with orifice plate cross-sectional structure, be rectangle, circle, rectangular or diamond shape.
7. the reagent evaporation retardation device according to claim 1 for fully automatic liquid processing, it is characterised in that: self-closing
Closing silica gel material is specially SS-6139.
8. the reagent evaporation retardation device according to claim 1 for fully automatic liquid processing, it is characterised in that: each
Silica gel sealing head is located above a reservoir, and the upper surface of silica gel sealing head and the distance range at the top of cover board are 1mm-
1.5mm。
9. the reagent evaporation retardation device according to claim 1 for fully automatic liquid processing, it is characterised in that: silica gel
The trench structure of seal head is circular trough, square groove, rectangular channel or diamond shape slot.
10. the inhibition side of -9 any reagent evaporation retardation devices for fully automatic liquid processing according to claim 1
Method, it is characterised in that: specific steps are as follows:
Before being loaded into automatic fluid processing equipment, cover board is covered on orifice plate；
All fluid treatment operations puncture cover board by liquid transfer gun head and realize.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910462886.4A CN110124764A (en) | 2019-05-30 | 2019-05-30 | Reagent evaporation retardation device and its suppressing method for fully automatic liquid processing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910462886.4A CN110124764A (en) | 2019-05-30 | 2019-05-30 | Reagent evaporation retardation device and its suppressing method for fully automatic liquid processing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110124764A true CN110124764A (en) | 2019-08-16 |
Family
ID=67583019
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910462886.4A Pending CN110124764A (en) | 2019-05-30 | 2019-05-30 | Reagent evaporation retardation device and its suppressing method for fully automatic liquid processing |
Country Status (1)
| Country | Link |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5789251A (en) * | 1994-06-16 | 1998-08-04 | Astle; Thomas W. | Multi-well bioassay tray with evaporation protection and method of use |
| US20060188404A1 (en) * | 2005-02-22 | 2006-08-24 | Gjerde Douglas T | Method and article for sealing a microplate |
| US20080116204A1 (en) * | 2006-11-20 | 2008-05-22 | Enplas Corporation | Container with lid |
| WO2010055939A1 (en) * | 2008-11-14 | 2010-05-20 | 信和化工株式会社 | Well plate with caps |
| CN203112813U (en) * | 2013-02-21 | 2013-08-07 | 生工生物工程（上海）股份有限公司 | PCR reaction device for preventing solution from evaporating |
| CN206671352U (en) * | 2017-04-10 | 2017-11-24 | 青岛市中心血站 | FAME reagent troughs |
| CN210385908U (en) * | 2019-05-30 | 2020-04-24 | 天津国科医工科技发展有限公司 | Reagent volatilization suppression device for full-automatic liquid treatment |
-
2019
- 2019-05-30 CN CN201910462886.4A patent/CN110124764A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5789251A (en) * | 1994-06-16 | 1998-08-04 | Astle; Thomas W. | Multi-well bioassay tray with evaporation protection and method of use |
| US20060188404A1 (en) * | 2005-02-22 | 2006-08-24 | Gjerde Douglas T | Method and article for sealing a microplate |
| US20080116204A1 (en) * | 2006-11-20 | 2008-05-22 | Enplas Corporation | Container with lid |
| WO2010055939A1 (en) * | 2008-11-14 | 2010-05-20 | 信和化工株式会社 | Well plate with caps |
| CN203112813U (en) * | 2013-02-21 | 2013-08-07 | 生工生物工程（上海）股份有限公司 | PCR reaction device for preventing solution from evaporating |
| CN206671352U (en) * | 2017-04-10 | 2017-11-24 | 青岛市中心血站 | FAME reagent troughs |
| CN210385908U (en) * | 2019-05-30 | 2020-04-24 | 天津国科医工科技发展有限公司 | Reagent volatilization suppression device for full-automatic liquid treatment |
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Effective date of registration: 20240401 Address after: 300000 building 4, No. 16, Wujing Road, Dongli Development Zone, Dongli District, Tianjin Applicant after: Tianjin Guoke Medical Technology Development Co.,Ltd. Country or region after: China Applicant after: Suzhou Institute of Biomedical Engineering and Technology Chinese Academy of Sciences Address before: Building 4, No.16 Wujing Road, development zone, Dongli District, Tianjin Applicant before: TIANJIN GUOKE YIGONG TECHNOLOGY DEVELOPMENT Co.,Ltd. Country or region before: China |
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