CN217893565U

CN217893565U - Cell culture liquid packaging bottle

Info

Publication number: CN217893565U
Application number: CN202221775934.9U
Authority: CN
Inventors: 蒋涛华; 傅坚刚; 邵悦
Original assignee: Nanjing Kebai Gene Technology Co ltd
Current assignee: Nanjing Kebai Gene Technology Co ltd
Priority date: 2022-07-11
Filing date: 2022-07-11
Publication date: 2022-11-25
Anticipated expiration: 2032-07-11

Abstract

The utility model provides a cell culture liquid packaging bottle. The cell culture solution packaging bottle comprises a bottle body and a sampling device. The utility model provides a cell culture liquid packaging bottle, set up the thread groove corresponding with flight one end on the stirring frisbee, the reel passes through bracing piece fixed connection with the stirring frisbee, can drive the flight when the barre removes and remove, and then can drive the stirring frisbee and rotate when the flight removes, the stirring frisbee rotates the cell culture liquid to the bottle with the reel simultaneously and stirs, it withdraws from the inside wall of bottle to drive the baffle through rotatory bolt, cell culture liquid in the bottle flows in the sample cell, rotate rotatory bolt once more and drive baffle and sealed inside wall that fits the bottle that fills up, cut off the space connection in sample cell and the bottle, then open the lid, carry out the sample check to cell culture liquid in the sample cell, be used for improving the leakproofness of bottle through setting up baffle and sealed the pad.

Description

Cell culture liquid packaging bottle

Technical Field

The utility model relates to a biological medicine field especially relates to a cell culture liquid packaging bottle.

Background

Biopharmaceuticals are a medical industry field that has been developed in the rise and explosion of the twenty-first century, and the main categories include recombinant protein drugs, monoclonal antibody drugs, and the like. Mammalian cell expression systems are becoming an increasingly important expression tool in order to obtain recombinant proteins in their native conformation and with correct glycoform modification.

Through the retrieval of publication numbers: CN213902946U discloses a cell culture solution packaging bottle, the sampling device comprises a sample cell, a rotary bolt, a cover, a bottle opening and a sealing film; the sample cell is positioned on the side surface of the bottle body and connected with the bottle body, the opening of the bottle body is positioned on the bottle body and is provided with threads, the sealing film is covered on the opening of the bottle body and plays a role in sealing, and the cover is covered on the opening of the sample cell; the rotary bolt is fixed on the wall of the sample cell through threads, and can puncture the sealing membrane through rotation or be in close fit with the threads of the opening of the bottle body through rotation; sample cell inner space and bottle inner space are airtight, when the cell culture liquid sample inspection to the bottle, can avoid the culture solution in the bottle to expose in the external world, prevent to pollute and can realize really, but unable automatic restoration after the film impales in this equipment, it is relatively poor only through the sealed effect of screw thread coincidence on rotatory bolt and the bottle opening, need shake the cell culture liquid in the bottle when using earlier evenly, if the condition that drops from the hand takes place easily for manual rocking.

Therefore, there is a need to provide a new cell culture solution packaging bottle to solve the above technical problems.

SUMMERY OF THE UTILITY MODEL

In order to solve the technical problem, the utility model provides a cell culture solution packaging bottle.

The utility model provides a cell culture liquid packaging bottle includes bottle and sampling device, the lateral wall of bottle is equipped with sampling device, and sampling device includes sample cell, rotary bolt and baffle, and sample cell one side is linked together with the through-hole of bottle one side, and the through-hole department of sample cell opposite side sets up screw thread, and rotary bolt and the screw thread sealing connection of sample cell through-hole department, and rotary bolt's one end runs through to be inserted in bottle one side and fixedly connected with baffle, and the sample cell is the closed with the cavity of bottle.

Further, the top of bottle has seted up the inlet respectively and has placed the hole, and the surface cover of inlet is equipped with the bottle lid, and the hole intussuseption of placing at bottle top is inserted and is had stirring subassembly.

Furthermore, a sampling port is formed in the top end of the sample pool, and a sealing cover is sleeved on the outer surface of the sampling port.

Furthermore, the circumferential direction of baffle encircles and is equipped with sealed the pad, and one side of sealed pad is laminated with the inside wall of bottle mutually.

Further, the stirring assembly comprises a column head, a handle bar, a spiral plate, a stirring flying disc and a shaft disc, two ends of the handle bar are respectively inserted into the bottom end of the column head and the placing hole of the bottle body, the spiral plate is fixedly connected to the other end of the handle bar, the surface of the spiral plate is sleeved with the stirring flying disc, the shaft disc is movably connected to the bottom end of the inner cavity of the bottle body, and a supporting rod is arranged between the shaft disc and the stirring flying disc.

Furthermore, a thread groove corresponding to one end of the spiral piece is formed in the stirring flying disc, and the shaft disc is fixedly connected with the stirring flying disc through a support rod.

Furthermore, the bottom of the column cap is sleeved with a return spring, and the return spring is fixedly connected with the top end of the bottle body.

Compared with the prior art, the utility model provides a cell culture liquid packaging bottle has following beneficial effect:

the utility model provides a cell culture liquid packaging bottle, when concrete implementation, set up the thread groove corresponding with flight one end on the stirring frisbee, the reel passes through bracing piece fixed connection with the stirring frisbee, can drive the flight when the barre removes, and then can drive the stirring frisbee and rotate when the flight removes, the stirring frisbee rotates the cell culture liquid in the bottle with the reel simultaneously, it withdraws from the inside wall of bottle to drive the baffle through rotatory bolt, cell culture liquid in the bottle flows in the sample cell, rotate the inside wall that rotatory bolt drove baffle and sealed pad laminating in the bottle once more, cut off the space connection in sample cell and the bottle, then open the lid, carry out the sampling inspection to the cell culture liquid in the sample cell, be used for improving the leakproofness of bottle through setting up baffle and sealed pad.

Drawings

FIG. 1 is a schematic structural view of a preferred embodiment of a cell culture solution packaging bottle according to the present invention;

FIG. 2 is a schematic cross-sectional structure of a cell culture fluid packaging bottle;

FIG. 3 is a schematic view of a sampling device;

fig. 4 is a schematic structural view of the stirring assembly.

Reference numbers in the figures: 1. a bottle body; 2. a sampling device; 3. a sample cell; 4. rotating the bolt; 5. a baffle plate; 6. a liquid inlet; 7. placing holes; 8. a bottle cap; 9. a stirring assembly; 10. a sampling port; 11. a sealing cover; 12. a gasket; 13. a column cap; 14. a handle bar; 15. a spiral sheet; 16. stirring the frisbee; 17. a reel; 18. a support bar; 19. a return spring.

Detailed Description

The present invention will be further described with reference to the accompanying drawings and embodiments.

Please refer to fig. 1, fig. 2, fig. 3 and fig. 4, wherein fig. 1 is a schematic structural diagram of a preferred embodiment of a cell culture solution packaging bottle according to the present invention; FIG. 2 is a schematic cross-sectional view of a cell culture solution packaging bottle; FIG. 3 is a schematic structural diagram of a sampling device; fig. 4 is a schematic structural view of the stirring assembly. The method comprises the following steps: a vial 1 and a sampling device 2.

In the specific implementation process, as shown in fig. 1-2, a sampling device 2 is disposed on the outer side wall of a bottle body 1, one side of a sample cell 3 is communicated with a through hole on one side of the bottle body 1, a through hole on the other side of the sample cell 3 is provided with a thread, a rotary bolt 4 is hermetically connected with the thread at the through hole of the sample cell 3, one end of the rotary bolt 4 is inserted into one side of the bottle body 1 and is fixedly connected with a baffle 5, and the cavities of the sample cell 3 and the bottle body 1 are both closed.

Referring to fig. 2 and 3, the top end of the bottle body 1 is respectively provided with a liquid inlet 6 and a placing hole 7, the outer surface of the liquid inlet 6 is sleeved with a bottle cap 8, the placing hole 7 at the top end of the bottle body 1 is internally inserted with a stirring component 9, the top end of the sample pool 3 is provided with a sampling port 10, the outer surface of the sampling port 10 is sleeved with a sealing cover 11, the circumferential direction of the baffle 5 is surrounded by a sealing gasket 12, one side of the sealing gasket 12 is attached to the inner side wall of the bottle body 1, the baffle 5 is driven by the rotating bolt 4 to move away from the inner side wall of the bottle body 1, the cell culture solution in the bottle body 1 flows into the sample pool 3, the rotating bolt 4 is rotated again to drive the baffle 5 and the sealing gasket 12 to be attached to the inner side wall of the bottle body 1, and the space connection between the sample pool 3 and the bottle body 1 is cut off.

Referring to fig. 2 and 4, two ends of a handle 14 are respectively inserted into the bottom end of a column cap 13 and the placing hole 7 of the bottle body 1, the other end of the handle 14 is fixedly connected with a spiral piece 15, a stirring flying disc 16 is sleeved on the surface of the spiral piece 15, a shaft disc 17 is movably connected to the bottom end of the inner cavity of the bottle body 1, a support rod 18 is arranged between the shaft disc 17 and the stirring flying disc 16, a thread groove corresponding to one end of the spiral piece 15 is formed in the stirring flying disc 16, the shaft disc 17 is fixedly connected with the stirring flying disc 16 through the support rod 18, a return spring 19 is sleeved at the bottom end of the column cap 13, the return spring 19 is fixedly connected with the top end of the bottle body 1, the stirring flying disc 16 and the shaft disc 17 rotate simultaneously to stir the cell culture solution in the bottle body 1, and the handle 14 and the spiral piece 15 are driven to move upwards slowly by the return spring 19 during the return.

The utility model provides a theory of operation as follows: before sampling, a handle 14 is driven to move downwards by pressing a column head 13, one end of the handle 14 is fixedly connected with a spiral sheet 15, a threaded groove corresponding to one end of the spiral sheet 15 is formed in a stirring flying disc 16, a shaft disc 17 is fixedly connected with the stirring flying disc 16 through a support rod 18, the spiral sheet 15 is driven to move when the handle 14 moves, the stirring flying disc 16 is driven to rotate when the spiral sheet 15 moves, the rotation principle refers to a toy 'flying fairy' hand-push flying disc, the stirring flying disc 16 and the shaft disc 17 rotate simultaneously to stir cell culture fluid in the bottle body 1, the handle 14 and the spiral sheet 15 are driven to move upwards slowly by rebounding of a return spring 19 when the stirring flying disc is reset, a baffle 5 is driven to move away from the inner side wall of the bottle body 1 by a rotating bolt 4, the rotating bolt 4 drives the baffle 5 and a sealing gasket 12 to be attached to the inner side wall of the bottle body 1, the space connection between the sample tank 3 and the bottle body 1 is cut off, then a cover is opened, the cell culture fluid in the sample tank 3 is sampled, and the baffle 5 and the sealing gasket 12 is arranged to improve the sealing performance of the bottle body 1.

The above only is the embodiment of the present invention, not limiting the scope of the present invention, all the equivalent structures or equivalent processes of the present invention are used in the specification and the attached drawings, or directly or indirectly applied to other related technical fields, and the same principle is included in the protection scope of the present invention.

Claims

1. The utility model provides a cell culture liquid packaging bottle, includes bottle (1) and sampling device (2), a serial communication port, the lateral wall of bottle (1) is equipped with sampling device (2), sampling device (2) include sample cell (3), rotatory bolt (4) and baffle (5), the through-hole of sample cell (3) one side and bottle (1) one side is linked together, and the through-hole department of sample cell (3) opposite side sets up the screw thread, the screw thread sealing connection of rotatory bolt (4) and sample cell (3) through-hole department, the one end of rotatory bolt (4) runs through to insert in bottle (1) one side and fixedly connected with baffle (5), the cavity of sample cell (3) and bottle (1) is the closed.

2. The cell culture solution packaging bottle according to claim 1, wherein a liquid inlet (6) and a placing hole (7) are respectively formed at the top end of the bottle body (1), a bottle cap (8) is sleeved on the outer surface of the liquid inlet (6), and a stirring component (9) is inserted into the placing hole (7) at the top end of the bottle body (1).

3. The cell culture solution packaging bottle according to claim 1, wherein a sampling port (10) is opened at the top end of the sample cell (3), and a sealing cover (11) is sleeved on the outer surface of the sampling port (10).

4. The packaging bottle for cell culture solution according to claim 1, wherein the baffle (5) is provided with a sealing gasket (12) around the circumference, and one side of the sealing gasket (12) is attached to the inner side wall of the bottle body (1).

5. The cell culture solution packaging bottle of claim 2, wherein the stirring assembly (9) comprises a column cap (13), a handle bar (14), a spiral plate (15), a stirring flying disc (16) and a shaft disc (17), two ends of the handle bar (14) are respectively inserted into the bottom end of the column cap (13) and the placing hole (7) of the bottle body (1), the spiral plate (15) is fixedly connected to the other end of the handle bar (14), the stirring flying disc (16) is sleeved on the surface of the spiral plate (15), the shaft disc (17) is movably connected to the bottom end of the inner cavity of the bottle body (1), and a supporting rod (18) is arranged between the shaft disc (17) and the stirring flying disc (16).

6. The packaging bottle of cell culture solution according to claim 5, wherein the stirring flying disc (16) is provided with a thread groove corresponding to one end of the spiral piece (15), and the shaft disc (17) is fixedly connected with the stirring flying disc (16) through a support rod (18).

7. The cell culture solution packaging bottle according to claim 5, wherein a return spring (19) is sleeved on the bottom end of the column head (13), and the return spring (19) is fixedly connected with the top end of the bottle body (1).