CN110123845A - A kind of polysaccharide composition and its preparation method and application of effective delaying senility function - Google Patents
A kind of polysaccharide composition and its preparation method and application of effective delaying senility function Download PDFInfo
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- 150000004676 glycans Chemical class 0.000 title claims abstract description 63
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 63
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 63
- 239000000203 mixture Substances 0.000 title claims abstract description 53
- 238000002360 preparation method Methods 0.000 title claims description 11
- 241000195493 Cryptophyta Species 0.000 claims abstract description 18
- 239000000843 powder Substances 0.000 claims abstract description 18
- 230000009758 senescence Effects 0.000 claims abstract description 15
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 11
- QKGJFQMGPDVOQE-UHFFFAOYSA-N Sulforaphen Natural products CS(=O)C=CCCN=C=S QKGJFQMGPDVOQE-UHFFFAOYSA-N 0.000 claims abstract description 9
- QKGJFQMGPDVOQE-HWKANZROSA-N raphanin Chemical compound CS(=O)\C=C\CCN=C=S QKGJFQMGPDVOQE-HWKANZROSA-N 0.000 claims abstract description 9
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 8
- 230000001934 delay Effects 0.000 claims abstract description 7
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims abstract description 6
- 235000010413 sodium alginate Nutrition 0.000 claims abstract description 6
- 229940005550 sodium alginate Drugs 0.000 claims abstract description 6
- 239000000661 sodium alginate Substances 0.000 claims abstract description 6
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/185—Vegetable proteins
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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- A61K31/21—Esters, e.g. nitroglycerine, selenocyanates
- A61K31/26—Cyanate or isocyanate esters; Thiocyanate or isothiocyanate esters
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/02—Algae
- A61K36/03—Phaeophycota or phaeophyta (brown algae), e.g. Fucus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
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- A—HUMAN NECESSITIES
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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- A61P39/06—Free radical scavengers or antioxidants
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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Abstract
The invention proposes the polysaccharide composition that one kind effectively delays senescence, the composition is component ratio fucoidan: sodium alginate: algae protein matter: sulforaphen=19:19:1:1.Above-mentioned polysaccharide composition can be used as flora and reconcile health care product, functional food, meal replacement powder etc., the polysaccharide composition has the beneficial effect that intestinal flora diversity can be improved in long-term stomach-filling, the composition of intestinal flora is reconciled, the growth of harmful intestinal tract flora is inhibited, maintains intestinal flora youth state；Nrf2/ARE signal path is activated, enhancing body is anti-oxidant, protects the ability of cell；Intensified response and sports coordination ability；The effect of food ration is had no significant effect, but mitigates its changes of weight, reaches anti-aging.
Description
Technical field
The present invention relates to biomedicine fields, and in particular to the polysaccharide composition and its preparation side that one kind effectively delays senescence
Method and application
Background technique
Aging refers to that the physiological function of each organ of body increases with the age and gradually fails, and causes disease and eventually leads to dead
It dies.Oxidative damage is the major incentive for causing aging, can significantly be delayed senescence by improving antioxidant ability of organism.Intestinal flora
Closely related with aging, enteric flora disturbance can cause whole body chronic inflammation, and then accelerate body aging.Due to intestinal flora with
Long-term diet style influences the presence of close ties, therefore, by adjusting diet style, had not only intervened intestinal microflora but also had improved
Antioxidant ability of organism, will be to delaying body aging to play a significant role.
Diet will affect the structure and composition of intestinal flora, therefore the intervention of diet is related caused by micropopulation imbalance
Disease provides an effective treatment method, and main component one of of the polysaccharide as diet is especially mentioned from plant or fungi
The polysaccharide taken out is proved to more have the title of " prebiotics " with the growth for adjusting intestinal microflora, promoting intestinal beneficial bacterium.
Brown alga sargassum fusifome sulfated polysaccharides rich in and in vivo necessary amino acid, are important dual-purpose of drug and food seaweed, have
Multiple biological activities, it is such as anti-oxidant, flora is adjusted, immune, hypoglycemic, reducing blood lipid and other effects is adjusted.
Summary of the invention
First mesh of the invention is to provide a kind of polysaccharide composition effectively to delay senescence, this is prebiotic for the prior art
First preparation, which has, adjusts intestinal flora, enhances the effect of antioxidant ability of organism and anti-aging.And the prebiotics are cell-free
Toxicity, securely and reliably, extraction process are simple, are suitable for industrialized production.
Another object of the present invention provides a kind of preparation method of polysaccharide composition effectively to delay senescence.
Another object of the present invention provides the application of the polysaccharide composition effectively to delay senescence.
Technical scheme is as follows: the polysaccharide composition that one kind effectively delays senescence, it is characterised in that: the group
Conjunction object is component ratio fucoidan: sodium alginate: algae protein matter: sulforaphen=19:19:1:1.
A kind of preparation method of the polysaccharide composition effectively to delay senescence, it is characterised in that there are following steps:
A.50 DEG C drying sargassum fusifome, is crushed into powder, crosses 20 mesh screens；
B. according to algae powder: 95% ethyl alcohol=1:5 (w:v) weighs and obtains sargassum fusifome algae powder in step 1, and fills with 95% ethyl alcohol
Point mixing, 70 DEG C reflux degreasing 3 times, each 2h, sufficiently slough the lipid and pigment in sargassum fusifome powder, acquisition degreasing sargassum fusifome
Algae powder；
C. distilled water is added into step 2 product according to solid-liquid ratio 1:20 (w:v), after stirring and leaching 12h, two layers of gauze mistake
Filter collects first time filtrate, distilled water in proportion is added in filter residue and continues to extract, and next every 2h collects filtrate, is repeated 3 times；
D. merging filtrate, 45 DEG C are concentrated into 1/10 or so, and it is 80%, 4 that 95% ethyl alcohol to alcohol concentration, which is added,
DEG C alcohol precipitation is stayed overnight；
E. alcohol precipitation mixed liquor is centrifuged 10min in 4000rpm, collects precipitating, and vacuum freeze drying obtains Hijiki polysaccharide,
Fucoidan in Hijiki polysaccharide: sodium alginate: the mass ratio of algae protein matter is 19:19:1.
F. Hijiki polysaccharide is mixed according to a certain percentage with sulforaphen, the ratio is sulforaphen
The mass ratio mixed with algae protein matter is 1:1.
The invention also discloses the application for the polysaccharide composition that one kind effectively delays senescence, the composite formula can be used for
Flora reconciles health care product, functional food, meal replacement powder.
The invention has the following beneficial effects: the present invention provides a kind of with anti-oxidant and adjusting flora function combination
Object, each component substance is compound in formula, generates synergistic effect, while providing a variety of nutrition to consumer, reaches more
The effect of good gastrointestinal tract health care especially delays senescence, the composition is in the functional food or drug that preparation delays senescence
Application, have and preferable reconcile intestinal flora enhancing antioxidant activity, delaying senility function, using above-mentioned Hijiki polysaccharide as former
Material, according to food or the conventional production process of medicine field, prepares the functionality for reconciling intestinal flora and anti-aging
Food or drug, long-term consumption can activate Nrf2/ARE signal path, enhancing antioxidant ability of organism and then protection cell, prolong
Slow body aging.The reagency that long-term consumption above-mentioned composition can enhance, coordination power improve locomitivity.
Detailed description of the invention:
Fig. 1 is influence of the polysaccharide composition to mouse weight (A) and food ration (B).
Fig. 2 is influence of the polysaccharide composition to mouse average response.
Fig. 3 is influence of the long-term stomach-filling polysaccharide composition to the mouse pole-climbing time.
Fig. 4 is influence of the long-term stomach-filling polysaccharide composition to mouse average hanging time.
Fig. 5 is related gene expression of the long-term stomach-filling polysaccharide composition to Nrf2/ARE signal path in mouse small intestine tissue
Horizontal influence.
Fig. 6 is long-term polysaccharide composition to the related gene expression for participating in Nrf2/ARE signal path in mouse liver tissue
Horizontal influence.
Fig. 7 is influence of the polysaccharide composition to intestinal flora Biodiversity Characteristics.
Fig. 8 is that polysaccharide composition is analyzed with Metastata method to different bacterium taxons spies in intestinal flora door level
Sign.
Specific embodiment
With reference to the accompanying drawings and examples, specific embodiments of the present invention will be described in further detail.Implement below
Example is not intended to limit the scope of the invention for illustrating the present invention.
The preparation process of polysaccharide composition:
1.50 DEG C of drying sargassum fusifomes, are crushed into powder, and cross 20 mesh screens；
2. according to algae powder: 95% ethyl alcohol=1:5 (w:v) weighs and obtains sargassum fusifome algae powder in step 1, and fills with 95% ethyl alcohol
Point mixing, 70 DEG C reflux degreasing 3 times, each 2h, sufficiently slough the lipid and pigment in sargassum fusifome powder, acquisition degreasing sargassum fusifome
Algae powder；
3. distilled water is added into step 2 product according to solid-liquid ratio 1:20 (w:v), after stirring and leaching 12h, two layers of gauze mistake
Filter collects first time filtrate, distilled water in proportion is added in filter residue and continues to extract, and next every 2h collects filtrate, is repeated 3 times；
4. merging filtrate, 45 DEG C are concentrated into 1/10 or so, it is 80%, 4 that 95% ethyl alcohol to alcohol concentration, which is added,
DEG C alcohol precipitation is stayed overnight；
5. alcohol precipitation mixed liquor is centrifuged 10min in 4000rpm, precipitating is collected, vacuum freeze drying obtains Hijiki polysaccharide,
Fucoidan in Hijiki polysaccharide: sodium alginate: the mass ratio of algae protein matter is 19:19:1.
6. Hijiki polysaccharide is mixed according to a certain percentage with sulforaphen, sulforaphen and algae protein matter are mixed
The mass ratio of conjunction is 1:1.
Beneficial effects of the present invention are further illustrated below by way of test example and test effect:
Test example 1: feeding polysaccharide composition is to mouse feed, the influence of weight and locomitivity:
The ICR mouse at male 12 monthly ages is divided into experimental group and control group at random, every group 20, single cage is raised in temperature
22-24 DEG C, relative humidity 40-60%, fluorescent lamp 12h illumination environment in.The daily stomach-filling polysaccharide composition 100mg/ of experimental group
Kg weight, control group stomach-filling same volume physiological saline, altogether stomach-filling 60 days, during which periodic logging mouse weight and food-intake.
Hanging test is carried out after stomach-filling 60 days: being horizontally arranged a metal iron net apart from ground 30cm, iron net lower section
Sawdust is spread on desktop, mouse hangs in iron net in experiment, the time (suspension time) of record mouse landing, more than 3 minutes
It is set to 3 minutes.Every mouse detects 3 times, every time detection interval 2 minutes.
Carry out Grasping clubglass test after stomach-filling 60 days: gauze is bound around iron stand prevents mouse from skidding, while pole top
Plug one piece of plank in portion.Mouse is head-up placed in the apical position of pole when experiment, mouse is recorded and turns direction head court
Under time (reaction time) and mouse climbed total time required for pole (pole-climbing time).Every mouse detects 3 times, often
Secondary detection is spaced 2min.
The decline of sports coordination ability is an important feature of aging, and the sports coordination ability of body can be with the increasing at age
It is long that downward trend is presented.By Fig. 2, Fig. 3 is it is found that be generally lower than control group small the average response of experimental mice and pole-climbing time
Mouse, and the suspension time of experimental mice is longer than control group.Fig. 4 also substantially meets above-mentioned phenomenon, and experimental mice is averaged
Reaction and pole-climbing time are generally lower than control group mice, and the suspension time of experimental mice is longer than control group.Illustrate polysaccharide
Composition produces certain promotion to the reaction of mouse and sports coordination ability.
Weight loss is one of important feature of aging.By Fig. 1 (A) it is found that compared with control group (stomach-filling physiological saline),
Experimental group (stomach-filling polysaccharide composition, 100mg/kg weight) mouse weight downward trend relatively mitigates.Food ration variation is also aging
One of index, downward trend can be presented with the growth at age in mouse food ration.
It is found by Fig. 1 (B), in this experiment, experimental group changes unobvious with control group mice food ration.Illustrate polysaccharide
Composition has an impact to mouse weight variation, and polysaccharide composition has effects that delay senescence.
The brown alga prebiotic preparation is eaten for a long time to have no significant effect mouse aging food ration, but its weight is delayed to become
Change, there is Nantural non-toxic, mild inirritative advantage.
Test example 2: feeding polysaccharide composition is to mouse intestinal flora structure:
If embodiment 2 raises animal, the excrement that the stomach-filling time is 0 day, 7 days, 14 days, 30 days and 60 days is collected, is used
Illumina HiSeq-PE250 high-flux sequence platform is to the mouse of stomach-filling polysaccharide composition and does not have stomach-filling polysaccharide composition
Mouse intestinal flora detected and analyzed.T group (stomach-filling polysaccharide is analyzed by UPGMA (Unifrac unweighted mean method)
Composition) and C group (stomach-filling physiological saline) mouse intestinal flora birds of the same feather flock together.
The brown alga prebiotic preparation, which is eaten for a long time, can be improved intestinal flora diversity, reconcile the composition of intestinal flora, suppression
The growth of harmful intestinal tract flora processed, and maintain intestinal flora youth state.
Enteric bacteria huge number, about the 10 of body cell times, by 3 kinds of physiological, opportunistic and pathogenicity bacterium groups
At.Existing research in recent years confirms that the stability and the normal physiological function of enteron aisle of intestinal flora are closely related, participates in regulation machine
The health and disease of body.Once the balance of intestinal flora is destroyed, the cytoprotection function of enteron aisle cannot run well.By
The protective capability of mouse aging intestinal cell can be repaired in intake polysaccharide composition for a long time.Therefore, we are taken the photograph for a long time by detection
Enter influence of the polysaccharide composition to the intestinal flora of mouse aging, establishes intestinal flora, polysaccharide composition, the relationship between aging
Net.To the mouse of stomach-filling polysaccharide composition and there is no stomach-filling polysaccharide with Illumina HiSeq-PE250 high-flux sequence platform
The mouse intestinal flora of composition is detected and is analyzed.
Group's species diversity (Community diversity) mainly by Shannon index and Simpson index Lai
It indicates.And the value of Shannon index and Simpson index is bigger, indicates that the diversity of population is higher.Existing research shows small
The richness and diversity of mouse intestinal flora are in significant downward trend with the raising at age.By high throughput sequencing technologies, to not
Diversity with the intestinal flora of mouse is detected, and as shown in Figure 7, compared with the control group, experimental mice intestinal flora is more
Sample is risen.The result shows that taken long-term polysaccharide composition can improve the diversity of mouse intestinal flora, improve enterobacteriaceae
Group structure.
It has been reported and points out Firmicutes (Firmicutes) and the relative abundance like bacillus door (Bacteroidetes)
(F/B) leading position [13] are occupied in mouse aging intestinal flora, and the group branch of Firmicutes is sent out in aging individuals
It is raw to change.T group (stomach-filling polysaccharide composition) and C group (stomach-filling physiology are analyzed by UPGMA (Unifrac unweighted mean method)
Salt water) mouse intestinal flora birdss of the same feather flock together.It is found from Fig. 8 A-B, in the mouse intestinal flora of stomach-filling polysaccharide composition, Firmicutes
Quantity decreases, and is increased like the quantity of bacillus door, i.e., Firmicutes are declined with the F/B value like bacillus door.Like bar
Bacterium door is primarily involved in polysaccharide metabolism, and Firmicutes are practically free of the enzyme of degradation of polysaccharide.Because of the digestive function of enteron aisle and more
Glycometabolism is closely related, and polysaccharide metabolic capability is reduced with the increase at age.Therefore, long-term stomach-filling Hijiki polysaccharide can reduce
F/B value in senile organism improves polysaccharide metabolic efficiency, makes mouse aging enteric microorganism component to young mice enteric microorganism
Component conversion.
By Fig. 8 C-D it is found that compared with the mouse of control group, pernicious bacteria such as cyanobacteria in experimental mice intestinal flora
Door (Actinobacteria) the quantity decline of (Cyanobacteria phyla), actinomyces.The result shows that taken long-term polysaccharide
Composition can inhibit the growth of harmful intestinal tract flora.
Test example 3: the influence of the anti-oxidant signal path of feeding polysaccharide composition Nrf2/ARE intracorporal to mouse machine:
After raising mouse 60 days, mouse is put to death, collects small intestine and liver organization, the measurement anti-oxidant signal of Nrf2/ARE is logical
The variation of road related gene expression.
Nowadays aging is generally considered accumulated as oxidative damage caused by, and enhance body anti-oxidation function approach it
One is exactly Nrf2/ARE signal path, cytoprotection and in terms of have critical role.Therefore, we pass through inspection
The relevant gene of Nrf2/ARE signal path is surveyed, cytoprotection to experimental group and control group mice small intestine, liver and stress
Defence capability is measured.As shown in Figure 5, compared with control group mouse aging, the Nrf2 and phase of experimental group mouse aging small intestine
Correlation gene significantly raises, and has extremely significant sex differernce (* * p < 0.01 and * * * p < 0.001=；It will be appreciated from fig. 6 that and control group mice
It comparing, the Nrf2 and related gene of experimental mice liver are significantly raised, and wherein Nrf2 has significant difference (* p < 0.05),
CAT, SOD-2, NQO-1 have extremely significant sex differernce (* * p < 0.01 and p < 0.001 * * *).The result shows that Nrf2/ARE signal path
Be activated, long-term consumption polysaccharide composition can enhance body it is anti-oxidant, protection cell ability, to reach the work of anti-aging
With.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, without departing from the technical principles of the invention, several improvements and modifications can also be made, above-mentioned hypothesis these
Improvement and modification are same to should be regarded as protection scope of the present invention.
Claims (3)
1. the polysaccharide composition that one kind effectively delays senescence, it is characterised in that: the composition is component ratio fucosan
Sulfuric ester: sodium alginate: algae protein matter: sulforaphen=19:19:1:1.
2. a kind of preparation method of the polysaccharide composition effectively to delay senescence in such as claim 1, it is characterised in that: have as follows
Step:
A.50 DEG C drying sargassum fusifome, is crushed into powder, crosses 20 mesh screens；
B. according to algae powder: 95% ethyl alcohol=1:5 (w:v) weighs and obtains sargassum fusifome algae powder in step 1, and sufficiently mixed with 95% ethyl alcohol
Close, 70 DEG C reflux degreasing 3 times, each 2h, sufficiently slough the lipid and pigment in sargassum fusifome powder, acquisition degreasing sargassum fusifome algae
Powder；
C. distilled water is added into step 2 product according to solid-liquid ratio 1:20 (w:v), after stirring and leaching 12h, two layers of filtered through gauze,
First time filtrate is collected, distilled water in proportion is added in filter residue and continues to extract, next every 2h collects filtrate, is repeated 3 times；
D. merging filtrate, 45 DEG C are concentrated into 1/10 or so, and it is 80% that 95% ethyl alcohol to alcohol concentration, which is added, 4 DEG C of alcohol
It is heavy to stay overnight；
E. alcohol precipitation mixed liquor is centrifuged 10min in 4000rpm, collects precipitating, and vacuum freeze drying obtains Hijiki polysaccharide, and sheep dwells
Fucoidan in dish polysaccharide: sodium alginate: the mass ratio of algae protein matter is 19:19:1；
F. Hijiki polysaccharide is mixed according to a certain percentage with sulforaphen, the ratio is sulforaphen and algae
The mass ratio of proteinoid mixing is 1:1.
3. a kind of application of the polysaccharide composition effectively to delay senescence in such as claims 1 or 2, it is characterised in that: the combination
Composition formula can be used for flora and reconcile health care product, functional food, meal replacement powder.
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