CN110122655A - A method of preparing high emulsibility egg white lotion - Google Patents
A method of preparing high emulsibility egg white lotion Download PDFInfo
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- CN110122655A CN110122655A CN201910403226.9A CN201910403226A CN110122655A CN 110122655 A CN110122655 A CN 110122655A CN 201910403226 A CN201910403226 A CN 201910403226A CN 110122655 A CN110122655 A CN 110122655A
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- egg white
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/04—Animal proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/22—Working-up of proteins for foodstuffs by texturising
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Abstract
The invention discloses a kind of method for preparing high emulsibility egg white lotion, it includes egg white pretreatment, the processing of Co-60 gamma ray, the preparation of addition organic acid, egg white lotion.The present invention solves the problems such as egg white lotion treatment process is cumbersome, emulsion stability is poor in conventional method, establishes a kind of easy, efficient, environmentally protective, safe and reliable and with high added value new method.
Description
Technical field
The present invention relates to a kind of preparation methods of high emulsibility egg white lotion, belong to food processing field.
Background technique
Compared with vegetable protein, albumen has superior nutritive value and is more advantageous to absorption of human body, egg white egg
White amino acid composition forms very close with essential amino acid.In addition to nutritive value is high, egg white also has many
Excellent processing characteristics, such as gelation, foaming characteristic and emulsibility.The gelation and foaming characteristic of egg white are in food processing field
(baking, dried eggs, surimi product) is widely used.But the albumen registered at present is in emulsification system
In application be severely limited to the pH value range of applicable system, under conditions of close to neutral or alkalinity, emulsibility
It is very unstable, it is also subject to certain restrictions in the application of field of food (such as Walnut Milk, ice cream, soymilk etc.).Albumen protein
Emulsibility it still needs further improvement.
Research shows that the major protein ovalbumin (54%-69% for accounting for albumen total amount) in egg white is by 385 ammonia
Base acid composition, wherein half amino acid is hydrophobic amino acid, and Most amino-acids can embed in the molecular structure, is unfavorable for egg
Clear emulsification, while egg white lotion is very unstable, is easy to happen layering demulsification.Had at present many researchs using enzymatic hydrolysis or
Polysaccharide is added in egg white solution to improve the emulsibility of egg white solution.Irradiation is a kind of cold sterilization technology, at the same time radiation treatment meeting
Also albumen protein can be made to generate denaturation or degradation, and then influence the functional character of protein.Improving food function characteristic
Aspect, some researches show that improve to radiation treatment energy conspicuousness the foaming power of egg white.But located using irradiation technique
The research that reason egg white solution improves its emulsifiability yet there are no report.
The present invention changes albumen protein structure in conjunction with pH processing is adjusted to develop a kind of and sterilization and function by radiation treatment
The preparation method for the high emulsibility egg white lotion that can improve, expands the application range of egg white food processing field.
Summary of the invention
The object of the present invention is to provide a kind of preparation methods of high emulsibility egg white lotion, mainly by carrying out spoke to egg white
According to processing, the emulsibility and stability of egg white lotion are improved in combination with other methods.
To achieve the above object, the invention adopts the following technical scheme:
A method of preparing high emulsibility egg white lotion, comprising the following steps:
(1) egg white of egg is separated with yolk and is chosen except frenulum in egg white, egg white is true with hermetic bag after mixing evenly
Empty package；
(2) egg white after packaging is irradiated with the source Co-60 γ, with the dosage rate irradiating sample of 3~5kGy/h, sample
Received dose of radiation is 1~5kGy；
(3) egg white after irradiation is taken out, the food grade organic acid that weight concentration is 1~10% is added into egg white, it will
The pH of egg white is adjusted to 3~6；
(4) egg white after pH will be adjusted soluble in water, is made into the egg white solution that weight concentration is 5~20%, then presses egg white solution
The 20~40% of volume are added soybean oil into egg white solution, first at room temperature with the revolving speed homogeneous 2 of 8000~9000rpm~
Then 10min uses high-pressure homogenizer further 30~40S of homogeneous under 30~40Mpa pressure, upper layer bubble is passed through gauze mistake
Filter is to get high emulsibility egg white lotion.
Preferably, the dose of radiation of the sample reception is 5kGy.
Preferably, the food grade organic acid is one of acetic acid, citric acid, malic acid or a variety of any combination.
Preferably, in step (4), the egg white after pH will be adjusted soluble in water, is made into the egg white solution that weight concentration is 10%, so
Soybean oil is added into egg white solution by the 30% of egg white solution volume afterwards.
The beneficial effects of the present invention are:
(1) present invention is handled by Co-60 gamma ray, it is therefore intended that induction egg white internal protein denaturation leads to albumen
Texture causes the hydrophobic variation of protein surface as changing.On the other hand, by destroying protein structure, egg is maintained
The stable intermolecular interaction of white matter starts to weaken, and emulsion viscosity is caused to reduce, the mobility enhancing of emulsion droplet.Most
Afterwards, radiation treatment can cause protein molecule fragment, and fracture, which occurs, for protein causes molecular weight to reduce, and size droplet diameter is smaller.
And irradiation process will not leave behind any residue, and entire treatment process is environmentally protective, safe and reliable.
(2) present invention is using addition organic acid, it is therefore intended that changes the alkaline environment of egg white.The pH of natural egg white is 7.6
Between 8.0, in neutral and alkaline conditions, most hydrophobic acidic amino acid residue is hidden in inside molecular structure, therefore very
On the surface, egg white shows the emulsifying capacity of strongly hydrophilic and difference for few exposure.In acid condition, the emulsification of ovalbumin is living
Property can improve.Meanwhile the organic acid of addition be all it is edible, egg white will not be polluted.
(3) present invention handles egg white using irradiation-organic acid, significantly improves the emulsifiability of egg white lotion, significantly
Stability of emulsion is improved, the lotion two-phase laminated flow time is postponed.Entire process flow is easy, compared with other production methods, this
Invent green no chemical residues, product will not be polluted, and substantially reduce the time, improve efficiency, reduce through
Ji cost, is suitable for enterprise's industrialized production.
Detailed description of the invention
Fig. 1 is the fluorescence microscopy figure of 1,2,3 egg white lotion of embodiment；
Fig. 2 is the laser scanning microscope figure of 1,2,3 egg white lotion of embodiment；
Fig. 3 is the grain size distribution of 1,2,3 egg white lotion of embodiment；
Fig. 4 is 1,2,3 egg white lotion of embodiment in delamination in different time periods.
Specific embodiment
Below by specific embodiment, the present invention is described in detail.
Embodiment 1
1) pretreatment of egg white:
Egg white is separated with yolk and is chosen except the frenulum in egg white, egg white is stirred evenly with electric mixer, is stirred
Mixing rate is 400rpm, then mixing time 30min is vacuum-packed with hermetic bag.
2) Co-60 gamma ray is handled:
The egg white after packing is irradiated in the source Co-60 γ supported with Hubei Prov. Acdemy of Agricutural Sciences, with the dosage rate of 3.4kGy/h
Irradiating sample, the dose of radiation of sample reception are 1kGy.
3) organic acid is added
Egg white after irradiation is taken out, the acetic acid that weight concentration is 1% is added into egg white, the pH of egg white is adjusted to 6；
4) preparation of egg white lotion
Egg white is soluble in water, it is made into the egg white solution that weight concentration is 15%, then by egg white solution volume 20% to egg
Soybean oil is added in clear liquid, first then uses high-pressure homogenizer at room temperature with 9000rpm homogeneous 2min with high speed shearing homogenizing device
The further homogeneous 40S under 30Mpa pressure, by upper layer bubble by filtered through gauze to get egg white lotion.
Embodiment 2
1) pretreatment of egg white:
The egg white of egg is separated with yolk and is chosen except frenulum in egg white, egg white is used to hermetic bag vacuum packet after mixing evenly
Dress.
2) Co-60 gamma ray is handled:
Egg white after packaging is irradiated with the source Co-60 γ, with the dosage rate irradiating sample of 4kGy/h, sample reception
Dose of radiation is 3kGy.
3) organic acid is added
Egg white after irradiation is taken out, the citric acid solution that weight concentration is 10% is added into egg white, adjusts solution
PH to 5.
4) preparation of egg white lotion
Egg white is soluble in water, it is made into the egg white solution that weight concentration is 6%, then by egg white solution volume 40% to egg white
Soybean oil is added in liquid, first with high speed shearing homogenizing device at room temperature with 8000rpm homogeneous 3min, is then existed with high-pressure homogenizer
Further homogeneous 40S under 30Mpa pressure, by upper layer bubble by filtered through gauze to get egg white lotion.
Embodiment 3
1) pretreatment of egg white:
The egg white of egg is separated with yolk and is chosen except frenulum in egg white, egg white is used to hermetic bag vacuum packet after mixing evenly
Dress.
2) Co-60 gamma ray is handled:
Egg white after packaging is irradiated with the source Co-60 γ, with the dosage rate irradiating sample of 4.5kGy/h, sample reception
Dose of radiation be 5kGy.
3) organic acid is added
Egg white after irradiation is taken out, the malic acid solution that weight concentration is 5% is added into egg white, adjusts the pH of solution
To 4.
4) preparation of egg white lotion
Egg white is soluble in water, it is made into the egg white solution that weight concentration is 10%, then by egg white solution volume 30% to egg
Soybean oil is added in clear liquid, first then uses high-pressure homogenizer at room temperature with 9000rpm homogeneous 3min with high speed shearing homogenizing device
The further homogeneous 30S under 30Mpa pressure, by upper layer bubble by filtered through gauze to get egg white lotion.
Test example
1, fluorescence microscopy
With the microscopic appearance of optical microscopy observation lotion.Suitable lotion is taken to be placed in centrifuge tube and dilute with distilled water
It releases, turned upside down is uniformly mixed.Then the lotion after taking a small amount of dilution is placed on glass slide, and gently covers glass slide, is prevented
Bubble generates.Select the microstructure of 10 × object lens and 40 × eyepiece observation lotion.As shown in Figure 1, the emulsion droplet of embodiment 1
Dispersibility is obviously improved than control egg white lotion, and the drop size observed becomes smaller, and the volume of blocky aggregation reduces.Embodiment
3 albumen emulsion droplet distribution is more compact, and the size of drop more tends to unanimously, and the dispersion effect of emulsion droplet is more preferable.
2, laser confocal microscope is analyzed
In order to observe the distribution situation of albumen and oil droplet in lotion, observed using laser confocal scanning microscope (CLSM)
The microstructure of lotion.Be added 100ul dilution after Nile red and 100ul dilution after FITC solution into 10ml egg white lotion,
It is uniformly mixed, is observed under the excitation wavelength of 488nm and the launch wavelength of 633nm.As seen from Figure 2, Examples 1 to 3 lotion
Drop is by a degree of protein encapsulation, and the emulsion droplet of embodiment 1 is bigger than normal and protein particulate aggregation extent is big, implements
The egg white emulsion droplet of example 2~3 is integrally on the high side and less than normal.
3, emulsion particle diameter measure of spread
Freshly prepared lotion is using light scattering particle size analyzer (Mastersizer2000) measurement partial size and partial size point
Cloth.Sample is diluted with a large amount of distilled water before measurement and avoids multiple scattering effect, set laser obscurity (5%-10%) with
And the refractive index of soybean oil, each sample, which is repeated three times, to be averaged.As shown in figure 3, at the 0th day, the grain of egg white lotion
Diameter is in unimodal normal distribution, and the particle diameter distribution of control group and embodiment 1 is between 10-500um, and the lotion of embodiment 2 and 3
Grain size is lower than 100um.This shows that the irradiation dose (3kgy, 5kgy) of high dose can cause the particle diameter of egg white lotion to become
It is small.For emulsion intercalation method dependent on granular size in lotion, emulsion particle is smaller to be more conducive to emulsion intercalation method.
4, cream analysis index analysis
Cream analysis measurement is to evaluate lotion with the stability of resting period.Freshly prepared lotion is divided in graduated
In test tube, stands, lotion is observed at regular intervals, record different time emulsification layer height (Hs) and total height
(Ht).As shown in figure 4, egg white lotion prepared by embodiment 1 is layered in two hours, egg prepared by embodiment 2 and embodiment 3
Clear lotion is layered at 6.5h and 10h respectively.In addition, as shown in table 1, the cream analysis index value of Examples 1 to 3 lotion is whole
It is upper to be less than blank group.
The cream analysis index table of 1 egg white lotion of table
5, the measurement of lotion Zeta potential
Use nanometer ZS Zetasizer (Malvern Instruments, Malvern, UK) measurement egg white lotion
Zeta potential.It adjusts egg white pH and egg white lotion deionized water is diluted 100 times, measurement carries out at 25 DEG C.As shown in table 4,
Within 0 to 15 days storage periods, the zeta potential value of the egg white lotion of blank group is about -16mV, the egg white cream of Examples 1 to 3
The zeta potential value conspicuousness of liquid increases (p < 0.05), and the net surface charge in albumen lotion increases, between protein body
Repulsive force increases, and emulsion intercalation method improves.
The zeta potential measurement result of 2 egg white lotion storage period of table
Claims (4)
1. a kind of method for preparing high emulsibility egg white lotion, it is characterised in that the following steps are included:
(1) egg white of egg is separated with yolk and is chosen except frenulum in egg white, egg white is used to hermetic bag vacuum packet after mixing evenly
Dress；
(2) egg white after packaging is irradiated with the source Co-60 γ, with the dosage rate irradiating sample of 3~5kGy/h, sample reception
Dose of radiation be 1~5kGy；
(3) egg white after irradiation is taken out, the food grade organic acid that weight concentration is 1~10% is added into egg white, by egg white
PH be adjusted to 3~6；
(4) egg white after pH will be adjusted soluble in water, is made into the egg white solution that weight concentration is 5~20%, then presses egg white solution volume
20~40% be added soybean oil into egg white solution, first at room temperature with revolving speed 2~10min of homogeneous of 8000~9000rpm, so
Afterwards use high-pressure homogenizer further 30~40S of homogeneous under 30~40Mpa pressure, by upper layer bubble pass through filtered through gauze to get
High emulsibility egg white lotion.
2. the method for preparing high emulsibility egg white lotion as described in claim 1, it is characterised in that: the radiation of the sample reception
Dosage is 5kGy.
3. the method for preparing high emulsibility egg white lotion as described in claim 1, it is characterised in that: the food grade organic acid is
One of acetic acid, citric acid, malic acid or a variety of any combination.
4. the method for preparing high emulsibility egg white lotion as described in claim 1, it is characterised in that: in step (4), after adjusting pH
Egg white it is soluble in water, be made into weight concentration be 10% egg white solution, then by egg white solution volume 30% into egg white solution plus
Enter soybean oil.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110742851A (en) * | 2019-11-12 | 2020-02-04 | 沈阳师范大学 | Aerated emulsion and preparation method and application thereof |
| CN112998223A (en) * | 2021-02-05 | 2021-06-22 | 上海海洋大学 | Shrimp paste and pre-emulsification preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110742851A (en) * | 2019-11-12 | 2020-02-04 | 沈阳师范大学 | Aerated emulsion and preparation method and application thereof |
| CN112998223A (en) * | 2021-02-05 | 2021-06-22 | 上海海洋大学 | Shrimp paste and pre-emulsification preparation method thereof |
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| CN110122655B (en) | 2021-08-20 |
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