CN110122496B - Pharmaceutical composition containing sodium dichloroisocyanurate and oxine-copper and application thereof - Google Patents

Pharmaceutical composition containing sodium dichloroisocyanurate and oxine-copper and application thereof Download PDF

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CN110122496B
CN110122496B CN201910532403.3A CN201910532403A CN110122496B CN 110122496 B CN110122496 B CN 110122496B CN 201910532403 A CN201910532403 A CN 201910532403A CN 110122496 B CN110122496 B CN 110122496B
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copper
oxine
sodium dichloroisocyanurate
pharmaceutical composition
mother liquor
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CN110122496A (en
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颜冬冬
曹坳程
王秋霞
李园
欧阳灿彬
郭美霞
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Institute of Plant Protection of Chinese Academy of Agricultural Sciences
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Institute of Plant Protection of Chinese Academy of Agricultural Sciences
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
    • A01N43/42—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings condensed with carbocyclic rings
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms

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  • Life Sciences & Earth Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention relates to a pharmaceutical composition containing sodium dichloroisocyanurate and oxine-copper and application thereof, belonging to the technical field of pesticide preparations. The pharmaceutical composition contains sodium dichloroisocyanurate and oxine-copper. The pharmaceutical composition can exert respective drug effects of sodium dichloroisocyanurate and oxine-copper to the maximum extent, can generate obvious synergistic effect on the aspect of preventing and controlling soil-borne pathogenic fungi and bacteria, reduces the dosage and the cost, and has obvious synergistic effect on bacterial wilt and phytophthora root rot.

Description

Pharmaceutical composition containing sodium dichloroisocyanurate and oxine-copper and application thereof
Technical Field
The invention relates to the technical field of pesticide preparations, in particular to a pharmaceutical composition containing sodium dichloroisocyanurate and oxine-copper for preventing and treating soil-borne pathogenic fungi and bacteria and application thereof.
Background
Soil-borne diseases are a class of plant diseases caused by infection with pathogens that survive in the soil. The plant cultivation method is serious in vegetables, melons and fruits and ornamental plants, and particularly serious in damage to crops in a greenhouse. Generally, after 3-5 years of greenhouse cultivation, the yield and quality of crops are seriously affected, generally, the yield is reduced by 20-40%, and the serious yield is reduced by more than 60% or even no harvest is caused. With the further expansion of the cultivation area of the greenhouse and the continuous cultivation of crops with high added value, the problem of soil-borne diseases is more and more prominent. Soil-borne diseases not only harm protected areas and high value-added crops, but also have serious harm to field crops such as soybeans, cotton, rice, wheat and the like. Soil disinfection treatment before crop planting can effectively prevent and control soil-borne diseases, but if the crops are damaged by the soil-borne diseases in the middle and later periods, no direct and effective method is available for control. At present, the pesticide can be directly used in the growth period of crops, and has a good control effect on soil-borne pathogenic fungi, bacteria and nematodes, and particularly, the pesticide lacks an effective pesticide variety on the pesticide for preventing and treating the soil-borne pathogenic bacterial diseases.
Disclosure of Invention
The invention aims to provide a pharmaceutical composition containing sodium dichloroisocyanurate and oxine-copper and application thereof. The pharmaceutical composition can exert respective drug effects of sodium dichloroisocyanurate and oxine-copper to the maximum extent, can generate obvious synergistic effect on the aspect of preventing and controlling soil-borne pathogenic fungi and bacteria, reduces the dosage and the cost, and has obvious synergistic effect on bacterial wilt and phytophthora root rot.
The invention provides a pharmaceutical composition, which contains sodium dichloroisocyanurate and oxine-copper.
Preferably, the pharmaceutical composition is obtained by mixing sodium dichloroisocyanurate mother liquor and oxine-copper mother liquor; the mass volume concentration of sodium dichloroisocyanurate in the sodium dichloroisocyanurate mother liquor is 10 mg/mL; the mass volume concentration of the oxine-copper in the oxine-copper mother solution is 10 mg/mL.
Preferably, the preparation of the sodium dichloroisocyanurate mother liquor comprises the following steps: dissolving sodium dichloroisocyanurate in water for dilution.
Preferably, the preparation of the oxine-copper mother liquor comprises the following steps: dissolving and diluting oxine copper with dimethyl sulfoxide.
Preferably, the volume ratio of the sodium dichloroisocyanurate mother liquor to the oxine-copper mother liquor is 1: 2-2: 1.
Preferably, the volume ratio of the sodium dichloroisocyanurate mother liquor to the oxine-copper mother liquor is 2: 1.
The invention also provides application of the pharmaceutical composition in the technical scheme in prevention and treatment of soil-borne pathogenic fungi and/or bacteria of crops.
The invention also provides application of the pharmaceutical composition in the technical scheme in preventing and treating crop bacterial wilt and/or root rot.
The invention provides a pharmaceutical composition, which contains sodium dichloroisocyanurate and oxine-copper. The pharmaceutical composition has potential synergistic effect, and by the compound use of sodium dichloroisocyanurate and oxine-copper, the control effect on target organisms can be effectively improved, particularly the control effect on soil-borne bacterial diseases can be improved, and the use amount of pesticides can be reduced. The test result shows that the pharmaceutical composition has better effect of preventing and treating the soil-borne diseases of the crops, less dosage and lower use cost, and has good application in preventing and treating soil-borne pathogenic fungi and bacteria of the crops.
Detailed Description
The invention provides a pharmaceutical composition, which contains sodium dichloroisocyanurate and oxine-copper. The pharmaceutical composition has potential synergistic effect, and by the compound use of sodium dichloroisocyanurate and oxine-copper, the control effect on target organisms can be effectively improved, particularly the control effect on soil-borne bacterial diseases can be improved, and the use amount of pesticides can be reduced.
In the invention, the pharmaceutical composition is obtained by mixing sodium dichloroisocyanurate mother liquor and oxine-copper mother liquor; the mass volume concentration of sodium dichloroisocyanurate in the sodium dichloroisocyanurate mother liquor is 10 mg/mL; the mass volume concentration of the oxine-copper in the oxine-copper mother solution is 10 mg/mL. The invention compounds the sodium dichloroisocyanurate mother liquor with the concentration of 10mg/mL and the oxine-copper mother liquor with the concentration of 10mg/mL, can exert the respective drug effects of the sodium dichloroisocyanurate and the oxine-copper to the maximum extent, greatly improves the activity of the pesticide, expands the control spectrum, greatly reduces the using dosage and the using cost of the pesticide, more effectively controls the soil-borne pathogenic fungi and bacterial diseases of crops than the two mother liquors are used independently, and particularly has better effect on the control of the bacterial wilt and root rot of the crops.
In the invention, the preparation of the sodium dichloroisocyanurate mother liquor comprises the following steps: dissolving sodium dichloroisocyanurate in water for dilution. The source of the sodium dichloroisocyanurate is not particularly limited, and the sodium dichloroisocyanurate can be obtained by adopting a conventional commercial product. In the present invention, the sodium dichloroisocyanurate is preferably a crude sodium dichloroisocyanurate drug having a purity of 91%, which is provided by Shanxi Kangpai Weiye Biotech Co., Ltd.
In the invention, the preparation of the oxine-copper mother liquor comprises the following steps: dissolving and diluting oxine copper with dimethyl sulfoxide. The source of the oxine-copper is not particularly limited in the invention, and the oxine-copper can be obtained by adopting a conventional commercial product. In the present invention, the oxine-copper is preferably an original oxine-copper drug with a purity of 95%, which is provided by Hainan chemical industry, Nantong, Inc.
In the invention, the volume ratio of the sodium dichloroisocyanurate mother liquor to the oxine-copper mother liquor is preferably 1: 2-2: 1. In the present invention, the volume ratio of the mixture of the sodium dichloroisocyanurate mother liquor and the quinoline copper mother liquor is more preferably 2: 1.
The invention also provides application of the pharmaceutical composition in the technical scheme in prevention and treatment of soil-borne pathogenic fungi and/or bacteria of crops.
The invention also provides application of the pharmaceutical composition in the technical scheme in preventing and treating crop bacterial wilt and/or root rot.
The pharmaceutical composition containing sodium dichloroisocyanurate and oxine-copper and the application thereof according to the present invention will be further described in detail with reference to the following specific examples, and the technical solutions of the present invention include, but are not limited to, the following examples.
Example 1
The pharmaceutical composition of the present invention was tested for its inhibitory effect against the bacterium Laurella solanacearum (Ralstonia solanacearum).
Step 1: preparation of medicinal liquid
Preparing a mother solution: 0.1007g of sodium dichloroisocyanurate original drug is respectively weighed by a ten-thousandth electronic balance and dissolved by water to prepare 100mL of mother solution of 1000 mu g/mL, 0.1003g of oxine-copper wettable powder is weighed and dissolved by dimethyl sulfoxide to prepare 100mL of mother solution of 1000 mu g/mL.
Step 2: the test strain is Ralstonia solanacearum, a bacterium belonging to the phylum Proteobacteria, the class of beta-Proteobacteria, the order of Burkholderia, the family of Burkholderia, and the genus Ralstonia.
And step 3: according to the agricultural industry standard (NY/T1156.16-2008) of the people's republic of China, the prevention and treatment effect of the medicament is measured by a turbidity method of a bacterial growth inhibition test.
The test strains grown on NA plates for 48 hours were eluted with sterile water to prepare 3 × 108CFU/mL bacterial suspension, 10. mu.L of 3 × 108CFU/mL of the bacterial solution is added into a 5mL centrifuge tube of 2mL LNB culture medium, and then different volumes of mother solution of the reagent to be tested are added to form a series of concentration gradients of 6.25 mu g/mL, 12.5 mu g/mL, 25 mu g/mL, 50 mu g/mL, 100 mu g/mL and 200 mu g/mL, and each process is repeated three times according to five ratios of the content of the effective components of the sodium dichloroisocyanurate and the oxine-copper and a blank control without the reagent.
And 4, step 4: and calculating and analyzing results. All treated bacteria liquid is subjected to ultraviolet spectrophotometry to determine the absorbance A under the wavelength of 600nm600The treated bacteria liquid is shake cultured in a shaking table at 180r/min and 28 ℃ until the concentration of the bacteria liquid is A600At 0.8-1.2 hours (i.e., into logarithmic growth phase), calibration was performed with NB medium containing only drug, and A was measured for each treatment600And calculating the antibacterial activity of each medicament.
According to the survey data, the growth inhibition rate of the bacteria was calculated according to formula (1) and expressed as a percentage (%), and the calculation results retained two digits after the decimal point.
Figure BDA0002100154400000041
In the formula: p, growth inhibition rate; a. the0Blank control turbidity increase value; the growth inhibition rate; a. the1The turbidity value of the medicament treatment is increased.
Using statistical software to make regression analysis of medicine concentration logarithmic value and control efficiency rate value to calculate medicine EC50、EC90And a 90% confidence interval.
The combined action type of the mixture of sodium dichloroisocyanurate and oxine-copper on ginger bacterial wilt is evaluated according to the Wadley method, the antagonism is achieved when the synergistic coefficient (SR) is less than 0.5, the addition effect is achieved when the SR is more than 0.5 and less than 1.5, and the synergism is achieved when the SR is more than 1.5. The synergistic coefficient (SR) is calculated according to the following formulas (2) and (3):
Figure BDA0002100154400000042
Figure BDA0002100154400000043
TABLE 1 indoor toxicity test results of the compounded preparation of the present invention against Ralstonia solanacearum
Figure BDA0002100154400000044
Figure BDA0002100154400000051
As can be seen from Table 1, the results of the indoor activity measurement show that the single agents EC50 of sodium dichloroisocyanurate and oxine-copper are respectively 27.75 μ g/mL and 23.92 μ g/mL, the content ratios of the active ingredients of the two agents are 1:1, 2:1, 1:2, 2:3 and 3:2, the medium inhibition concentrations EC50 of the growth amount of ginger bacterial wilt pathogenic bacteria are respectively 10.93, 10.60, 11.24, 15.95 and 14.30 μ g/mL, the synergistic coefficients are respectively 2.35, 2.48, 2.23, 1.59 and 1.82, and the synergistic effects are respectively shown, wherein the sodium dichloroisocyanurate and the oxine-copper have better inhibition effects on the bacterial wilt under the condition that the sodium dichloroisocyanurate and the oxine-copper are compounded under the condition that the ratio is 2: 1.
Example 2
The pharmaceutical composition provided by the invention is used for testing the inhibition effect of phytophthora capsici (Phytohthora capsicii).
The steps 1 and 2 of the test and the calculation method of the synergistic effect are basically the same as the first embodiment, wherein the step 3 is to measure the control effect of the medicament by adopting a hypha growth rate method according to the agricultural industry standard (NY/T1156.2-2008) of the people's republic of China.
Selecting bacterial colonies with consistent growth vigor from cultured phytophthora capsici by using a sterilization puncher with the diameter of 7mm under the aseptic operation condition, cutting off bacterial cakes from the edges of the bacterial colonies, inoculating the bacterial cakes to the center of a drug-containing flat plate by using an inoculator, covering a dish cover with the hypha facing downwards, and placing the dish cover in an incubator at 25 ℃ for dark culture.
And 4, step 4: and calculating and analyzing results. And (3) investigating the growth condition of the hyphae of the pathogenic bacteria according to the growth condition of the colonies in the blank control culture dish, measuring the diameter of each processed colony by a cross method after the colonies in the blank control grow sufficiently, calculating the growth diameter of the colony by adopting a formula (4), and taking the average value of the growth diameters.
Colony growth diameter-colony diameter-fungus cake diameter (4)
The inhibition rate of the test result is calculated by the formula (5), and the inhibition rate of each medicament treatment on the growth of the hyphae of the phytophthora capsici is calculated by the growth diameter of the blank control colony and the growth diameter of the medicament treated colony.
Figure BDA0002100154400000052
Analyzing the test data by regression analysis method, converting the inhibition rate of hypha growth into several values, calculating EC by linear regression analysis method with logarithmic value of each drug concentration as abscissa50Value, the magnitude of virulence of each treatment agent was compared. The synergistic effect was calculated as in example one.
TABLE 2 indoor toxicity test results of the compounded preparation of the present invention against Fusarium oxysporum
Figure BDA0002100154400000061
As can be seen from Table 2, sodium dichloroisocyanurate and oxine-copper are used for inhibiting the growth rate of phytophthora capsici hyphae50622.25 mug/mL and 676.89 mug/mL respectively, when the sodium dichloroisocyanurate and the oxine-copper are mixed according to the proportion of 2:1, the concentration EC50 for inhibiting the growth rate of the phytophthora capsici mycelium is 397.46 mug/mL, and the synergistic coefficient is as follows: 1.61, the synergistic effect is shown, so that the sodium dichloroisocyanurate and the oxine-copper are compounded at the ratio of 2:1 to have good inhibition effect on phytophthora capsici.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims (3)

1. The application of the pharmaceutical composition in controlling the bacterial wilt of crops is characterized in that the pharmaceutical composition consists of sodium dichloroisocyanurate and oxine-copper; the pharmaceutical composition is obtained by mixing sodium dichloroisocyanurate mother liquor and oxine-copper mother liquor; the mass volume concentration of sodium dichloroisocyanurate in the sodium dichloroisocyanurate mother liquor is 10 mg/mL; the mass volume concentration of the oxine-copper in the oxine-copper mother solution is 10 mg/mL; the volume ratio of the sodium dichloroisocyanurate mother liquor to the quinoline copper mother liquor is 2: 1.
2. The use according to claim 1, characterized in that the preparation of said mother liquor of sodium dichloroisocyanurate comprises the following steps: dissolving sodium dichloroisocyanurate in water for dilution.
3. The use according to claim 1, wherein the preparation of the quinolinone copper mother liquor comprises the following steps: dissolving and diluting oxine copper with dimethyl sulfoxide.
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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101103728A (en) * 2007-08-17 2008-01-16 王安林 Agricultural sterilizing and disinfecting composition, preparation method and application thereof
CN102823603A (en) * 2012-09-13 2012-12-19 王学权 Sterilization compound containing chloroisobromine cyanuric acid
CN104140646A (en) * 2014-07-17 2014-11-12 滁州优胜高分子材料有限公司 Halogen-free antioxidant pbt material

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9371479B2 (en) * 2011-03-16 2016-06-21 Schlumberger Technology Corporation Controlled release biocides in oilfield applications

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101103728A (en) * 2007-08-17 2008-01-16 王安林 Agricultural sterilizing and disinfecting composition, preparation method and application thereof
CN102823603A (en) * 2012-09-13 2012-12-19 王学权 Sterilization compound containing chloroisobromine cyanuric acid
CN104140646A (en) * 2014-07-17 2014-11-12 滁州优胜高分子材料有限公司 Halogen-free antioxidant pbt material

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