CN110117534A - A kind of PCR amplification detector - Google Patents
A kind of PCR amplification detector Download PDFInfo
- Publication number
- CN110117534A CN110117534A CN201910322425.7A CN201910322425A CN110117534A CN 110117534 A CN110117534 A CN 110117534A CN 201910322425 A CN201910322425 A CN 201910322425A CN 110117534 A CN110117534 A CN 110117534A
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- radiator
- plano
- pcr
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- pcr amplification
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- 238000012408 PCR amplification Methods 0.000 title claims abstract description 28
- 239000011159 matrix material Substances 0.000 claims abstract description 24
- 230000003287 optical effect Effects 0.000 claims description 24
- 239000004411 aluminium Substances 0.000 claims description 11
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical group [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims description 11
- 229910052782 aluminium Inorganic materials 0.000 claims description 11
- 238000001514 detection method Methods 0.000 claims description 9
- 239000000463 material Substances 0.000 claims description 8
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 4
- 230000017525 heat dissipation Effects 0.000 claims description 3
- 230000005540 biological transmission Effects 0.000 claims 1
- 230000008859 change Effects 0.000 abstract description 6
- 239000007787 solid Substances 0.000 abstract description 6
- 238000000034 method Methods 0.000 abstract description 4
- 230000035484 reaction time Effects 0.000 abstract description 4
- 230000003321 amplification Effects 0.000 abstract description 3
- 230000004907 flux Effects 0.000 abstract description 3
- 238000003199 nucleic acid amplification method Methods 0.000 abstract description 3
- 230000008569 process Effects 0.000 abstract description 3
- 238000006243 chemical reaction Methods 0.000 description 11
- 229910052751 metal Inorganic materials 0.000 description 4
- 239000002184 metal Substances 0.000 description 4
- 238000009423 ventilation Methods 0.000 description 4
- 238000001816 cooling Methods 0.000 description 3
- 238000003384 imaging method Methods 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 239000000376 reactant Substances 0.000 description 2
- 238000009738 saturating Methods 0.000 description 2
- 238000002834 transmittance Methods 0.000 description 2
- 230000003044 adaptive effect Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 239000012295 chemical reaction liquid Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The invention discloses a kind of PCR amplification detector, including result reading module, temperature control modules and shell, housing upper face is equipped with light-transmitting plate, and temperature control modules are set in the housing, and as a result reading module is set to above light-transmitting plate.There is ready temperature region in PCR amplification detector shell of the invention, the high temperature air of temperature region or Cryogenic air are directly blown into PCR pipe matrix and contact generation heat transfer with PCR pipe by centrifugal fan, thus, 100% contact heat conductien is realized in contact of the solid with gas, rate temperature change faster, can be improved more than 8 DEG C per second or more, significantly shorten the reaction time of entire amplification process in this way, experimental cost is reduced, and then the PCR for improving equipment reacts flux.
Description
Technical field
The present invention relates to PCR amplification technical field, especially a kind of PCR amplification detector.
Background technique
Currently, the high/low temperature control of domestic PCR amplification instrument all uses metal contact, use Peltier as height temperature control
Source processed belongs to contact PCR instrument；The result interpretation mode of PCR amplification instrument includes: a, naked eyes interpretation, passes through shade interpretation
Yin and yang attribute；B, using fiber optic conduction absorbance interpretation.And absorbance interpretation is used, it is black since reactant has black floating material
Color floating material also can extinction, reactions can only be waited to complete to can be only achieved relatively high interpretation accuracy after precipitating in this way.
Contact PCR instrument, using the temperature of reaction solution in Peltier control PCR pipe, due to Peltier temperature changing speed
It is slow, PCR pipe and Peltier directly across a metal vessel, metal vessel increase one layer it is thermally conductive, further extend
The temperature change period；And PCR pipe and metal vessel are all solids, contact area can not accomplish that 100% heating power conducts, heating and cooling
The limit can not be broken through, highest 5 DEG C of rate temperature change per second needs the long reaction time, and positive and negative warm efficiency
It is low, since longer service life makes experimental cost height.The result interpretation of contact PCR instrument, due to the height of PCR longer time
Temperature reaction can accelerate the degree of aging of interpretation sensor, shorten instrument service life.
Summary of the invention
Based on the above issues, it is an object of the invention to overcome in place of above-mentioned the deficiencies in the prior art and providing one kind can show
It writes and improves reacting liquid temperature pace of change in PCR pipe, shorten the reaction time of amplification process, reduce experiment originally, same to time delay
Long instrument service life.
To achieve the above object, the technical scheme adopted by the invention is as follows: a kind of PCR amplification detector, including result interpretation
Module, temperature control modules and shell, the housing upper face are equipped with light-transmitting plate, and the temperature control modules are set to the shell
Interior, the result reading module is set to above the light-transmitting plate；The temperature control modules include PCR pipe matrix, the first heat dissipation
Device, Peltier, support plate, high temperature air area, target upper temperatures air zone, Cryogenic air area, target low air zone and fan, institute
The pedestal for stating the first radiator is fixedly connected with the side wall of the shell, is formed between first radiator and case top close
Space is closed, the support plate is fixedly connected with first radiator, and the support plate is located above first radiator, institute
PCR pipe matrix is stated in the support plate, the PCR pipe matrix is located at below the light-transmitting plate, the Peltier with it is described
It is closely connected below the pedestal of first radiator, the high temperature air area, target upper temperatures air zone, Cryogenic air area, target are low
Warm air differentiation is distributed in around the PCR pipe matrix, and high temperature air area, target upper temperatures air zone, Cryogenic air area, target
Cryogenic air area is respectively equipped with fan.PCR amplification detector of the invention uses 4 different temperatures air-sources as a result, passes through gas
The PCR pipe heat exchange of body and solid has faster temperature rate；High temperature air area, target upper temperatures air zone, Cryogenic air
Area, the region of target low air zone four are equipped with independent Peltier temperature control module, temperature detecting module, four when normal work
The temperature that aluminium block on region is kept constant, only the static air in the region keeps identical temperature, when the corresponding pumping of unlatching
When wind, the air of circulation is contacted with aluminium block, and due to heat transfer, air is also heated to corresponding temperature.
As advanced optimizing for above scheme, the result reading module includes optical path and detection circuit, the detection
Multiple color sensors that circuit includes circuit board and connect with circuit board, the multiple color sensor are set in a row, often
An optical path, the longitudinally disposed three optical imagery eyeglasses composition of every optical routing are equipped with below one color sensor.
PCR amplification detector of the invention uses contactless full-automatic result interpretation as a result, by optical focusing image-forming principle,
The reaction color of more accurate analysis reaction liquid, realizes quick interpretation result, interpretation result accuracy can be improved；Separately
It outside, can row's PCR pipe reaction result below interpretation by mobile row's color sensor；Unit area PCR pipe matrix can
To accommodate more PCR pipes, pcr amplification reaction flux is high.
As advanced optimizing for above scheme, the second radiator, the bottom of second radiator are equipped in the shell
Seat is closely connect with the Peltier.
As advanced optimizing for above scheme, radiator fan, the housing bottom are equipped with below second radiator
Equipped with heat dissipating grid, the heat dissipating grid is located at the lower section of the radiator fan.
As advanced optimizing for above scheme, the material of first radiator is aluminium.
As advanced optimizing for above scheme, the material of second radiator is aluminium.
As advanced optimizing for above scheme, the high temperature air area, target upper temperatures air zone and the Cryogenic air
Area, target low air zone are respectively arranged on the opposite two sides of the PCR pipe matrix.It should be noted that existing PCR pipe temperature
It is to be contacted with PCR pipe by Peltier, aluminium block to realize temperature control, in this way due to solid and solid contact low efficiency, temperature control lag
Bigger, PCR pipe and aluminium block temperature delay are bigger；Temperature control modules are divided into 4 regions by the present invention, so as to higher
Some regional air is first extracted into PCR pipe conversion zone by heat transfer when the temperature difference and PCR pipe contact, and realization is rapidly heated (cooling),
It is then switched off high-temperature region (or low-temperature space), opens simultaneously target upper temperatures area (or low-temperature space)；Target upper temperatures area and target low area,
The temperature in the region is consistent with the target temperature of PCR pipe reaction zone, as long as keeping the temperature in the good region that PCR can be realized in this way
The temperature of pipe accurately controls, as long as keeping the air circulation in target temperature area；In order to reduce power consumption, the present invention is by high-temperature region
PCR pipe matrix two sides are disposed on low-temperature space, high-temperature region is far from low-temperature space, to reduce heat transfer between aluminium block.
As advanced optimizing for above scheme, three longitudinally disposed optical imagery eyeglasses are followed successively by from top to bottom
First plano-convex lens, the second plano-convex lens and third plano-convex lens, wherein the first plano-convex lens, the second plano-convex lens and third
The plane of plano-convex lens is all arranged in parallel with the circuit board, and first plano-convex lens are flat close to the one side of the circuit board
Face, second plano-convex lens and third plano-convex lens close to the circuit board are all convex surface on one side.It should be noted that three
A plano-convex lens are all high transmittance material convex lenses, and specification is identical or different to be ok, and can be determined according to optical path height
The parameter of three eyeglasses, if highest optical transmittance may be implemented in three convex lens using same size；Following two is convex
Mirror (i.e. the second plano-convex lens and third plano-convex lens) determines imaging field of view and focusing position, and the visual field is allowed to concentrate on PCR
It is topmost imaging convex lens close to the convex lens of RGB sensor (i.e. the first plano-convex lens), which can basis in tube reaction object
RGB photosensitive area size is incident upon reaction object image installation maximum area on RGB sensor (i.e. color sensor), in this way
The optical imagery of peak efficiency can be obtained.
In conclusion the invention has the benefit that
There is ready temperature region in PCR amplification detector shell of the invention, centrifugal fan is by the height of temperature region
Warm air or Cryogenic air are directly blown into PCR pipe matrix and contact generation heat transfer, as a result, contact of the solid with gas with PCR pipe
Realize that 100% contact heat conductien, rate temperature change faster, can be improved more than 8 DEG C per second or more, can significantly shorten in this way
The reaction time of entire amplification process reduces experimental cost, and then the PCR for improving equipment reacts flux.
Detailed description of the invention
Fig. 1 is that the air in the present invention around PCR pipe matrix flows schematic illustration；
Fig. 2 is the temperature control principle schematic diagram of temperature control modules in the present invention；
Fig. 3 is the structural schematic diagram of PCR amplification detector of the invention；
Fig. 4 is the top view of PCR amplification detector of the invention；
Fig. 5 is a kind of side view of embodiment of PCR amplification detector of the invention；
Fig. 6 is the another side side view of PCR amplification detector of the invention；
Wherein, 1, shell, 2, light-transmitting plate, 3, PCR pipe matrix, the 4, first radiator, 5, Peltier, 6, support plate, 7, height
Warm air area, 8, target upper temperatures air zone, 9, Cryogenic air area, 10, target low air zone, 11, fan, 12, circuit board,
13, color sensor, the 14, first plano-convex lens, the 15, second radiator, 16, radiator fan, 17, heat dissipating grid, 18, ventilation
Hole, the 19, second plano-convex lens, 20, third plano-convex lens, 21, baffle, 22, PCR pipe, 23, high-temperature region, 24, low-temperature space；Fig. 1,3
Arrow shows air-flow direction in~6.
Specific embodiment
To better illustrate the object, technical solutions and advantages of the present invention, below in conjunction with the drawings and specific embodiments pair
The present invention is described further.
Embodiment 1
As shown in Figures 1 to 3, a kind of embodiment of PCR amplification detector of the invention, including result reading module, temperature
Control module and shell 1, shell 1 are equipped with light-transmitting plate 2 above, and temperature control modules are set in shell 1, and as a result reading module is set to
2 top of light-transmitting plate；Temperature control modules include PCR pipe matrix 3, the first radiator 4, Peltier 5, support plate 6, high temperature air area
7, target upper temperatures air zone 8, Cryogenic air area 9, target low air zone 10 and fan 11, the pedestal and shell of the first radiator 4
The side wall of body 1 is fixedly connected, and the first radiator 4 and shell form confined space, support plate 6 and the first radiator 4 between 1 top
It is fixedly connected, support plate 6 is located at 4 top of the first radiator, and PCR pipe matrix 3 is set in support plate 6, and PCR pipe matrix 3 is located at saturating
The lower section of tabula rasa 2 is closely connect below Peltier 5 and the pedestal of the first radiator 4, high temperature air area 7, target upper temperatures air zone 8,
Cryogenic air area 9, target low air zone 10 are distributed in around PCR pipe matrix 3, and high temperature air area 7, target upper temperatures air
Area 8, Cryogenic air area 9, target low air zone 10 are respectively equipped with fan 11；High temperature air area 7,8 and of target upper temperatures air zone
Cryogenic air area 9, target low air zone 10 are respectively arranged on the opposite two sides of PCR pipe matrix 3；
As a result reading module includes optical path and detection circuit, and detection circuit includes circuit board 12 and connect with circuit board 12
Multiple color sensors 13, multiple color sensors 13 set in a row, and a light is equipped with below each color sensor 13
Road, the longitudinally disposed three optical imagery eyeglasses composition of every optical routing；Three longitudinally disposed optical imagery eyeglasses from up to
Under be followed successively by the first plano-convex lens 14, the second plano-convex lens 19 and third plano-convex lens 20, wherein the first plano-convex lens 14,
The plane of two plano-convex lens 19 and third plano-convex lens 20 is all arranged in parallel with circuit board 12, and saturating 14 mirror of the first plano-convex is close to circuit
The one side of plate 12 is plane, and the second plano-convex lens 19 and third plano-convex lens 20 close to circuit board 12 are all convex surface on one side；
The second radiator 15 is additionally provided in shell, the pedestal of the second radiator 15 is closely connect with Peltier 5；Second heat dissipation
16 are equipped with below device 15,1 bottom of shell is equipped with heat dissipating grid 17, and heat dissipating grid 17 is located at the lower section of radiator fan 16, and radiate grid
Lattice 17 are equipped with ventilation hole 18；The material of first radiator 4 and the second radiator 15 is aluminium.
The specific working principle of PCR amplification detector of the present embodiment is as follows:
(1) temperature divides into four temperature control regions, and high temperature air area, Cryogenic air area, target upper temperatures air zone, target are low
Warm air area, according to the high/low temperature temperature of user setting, this 4 temperature automatic constant-temperature before equipment experiment starts；
When needing to heat up, first with radiator fan circulation high temperature air, PCR pipe is set to be rapidly heated, then pass to high-temperature targets
The air of humidity province makes PCR pipe reach the temperature of needs；
When needing to cool down, first with the air of radiator fan circulation low-temperature region, until PCR pipe fast cooling, then pass to
The air of Low Temperature Target humidity province makes PCR pipe reach the low temperature of needs；
As shown in Fig. 2, passing through Intelligent PID Control fan speed and high temperature air area 7, target upper temperatures air zone 8, low temperature
The temperature change of air zone 9, target low air zone 10.Intelligent PID Control is by intelligent control (intelligent
Control it) is combined with traditional PID control, is adaptive.
(2) color interpretation:
As a result reading module is made of optical path and detection circuit, and optical path is placed in the top of PCR pipe, multiple optical paths (such as 8
It is a) it can move, it can detect the PCR reaction result of a row simultaneously every time, can detecte multiple row by mobile imaging lens
PCR pipe；Using the optical imagery optical path of profession, the reactant middle position of PCR pipe bottom is scheduled at image focus, it in this way can be with
Reduce external disturbance；
Using the PCR pipe of a row as the detection unit of single, the PCR pipe of a row can be 1~n quantity, and movement is once sentenced
Read through model detects the PCR pipe of a row；Optical path and circuit board are installed together by screw, not in the shell of PCR instrument, detection
Unit is an independent part, is placed on above shell；PCR thermotonus is detached from reading module and also can independently work normally；
(3) the positive temperature region of Peltier is different with the temperature of reverse side temperature region, by changing Peltier pin
Voltage direction, thus it is possible to vary the temperature difference of front and back sides；Each temperature region is by radiator fan, aluminium radiator, a Paar
Patch, control circuit composition；Four humidity provinces are all independent；Cross-ventilation is realized by the ventilation hole in heat dissipating grid, in turn
It radiates to the second radiator another side；
The air of Peltier front and back sides is completely isolated, and Peltier upper air is come out from humidity province, by PCR pipe, again
Return to humidity province；Air below Peltier is connected with environment, is directly carried out with the air of environment to the second radiator scattered
Heat.
Embodiment 2
A kind of embodiment of PCR amplification detector of the invention, the difference from embodiment 1 is that: high temperature air area 7, mesh
Absolute altitude warm air area 8, Cryogenic air area 9, the position of target low air zone 10 are different；As shown in figure 4,7 He of high temperature air area
Target upper temperatures air zone 8, Cryogenic air area 9 and target low air zone 10 are located at the adjacent two sides of PCR pipe matrix 3；Fig. 5
High temperature area 23 (including high temperature air area 7 and target upper temperatures air zone 8) and low-temperature space 24 (including Cryogenic air area 9 and target
Cryogenic air area 10) it is located at the adjacent two sides of PCR pipe matrix 3, it is connected with baffle 21 at the top of target upper temperatures air zone 8, for limiting
The flow direction of target upper temperatures air processed targets the bottom PCR；Show that Cryogenic air by PCR pipe matrix, surrounds support plate in Fig. 6
In shell internal circulation flow.
Finally it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than protects to the present invention
The limitation of range, although the invention is described in detail with reference to the preferred embodiments, those skilled in the art should be managed
Solution, can with modification or equivalent replacement of the technical solution of the present invention are made, without departing from technical solution of the present invention essence and
Range.
Claims (8)
1. a kind of PCR amplification detector, which is characterized in that including result reading module, temperature control modules and shell, the shell
Light-transmitting plate is equipped with above body, the temperature control modules are disposed in the housing, and the result reading module is set to the light transmission
Above plate；
The temperature control modules include PCR pipe matrix, the first radiator, Peltier, support plate, high temperature air area, target height
Warm air area, Cryogenic air area, target low air zone and fan, the pedestal of first radiator and the side wall of the shell
It is fixedly connected, forms confined space, the support plate and first radiator between first radiator and case top
It is fixedly connected, the support plate is located above first radiator, and the PCR pipe matrix is set in the support plate, described
PCR pipe matrix is located at below the light-transmitting plate, closely connect below the Peltier and the pedestal of first radiator, described
High temperature air area, target upper temperatures air zone, Cryogenic air area, target low air zone are distributed in around the PCR pipe matrix,
And high temperature air area, target upper temperatures air zone, Cryogenic air area, target low air zone are respectively equipped with fan.
2. PCR amplification detector according to claim 1, which is characterized in that the result reading module include optical path and
Detection circuit, multiple color sensors that the detection circuit includes circuit board and connect with circuit board, the multiple color
Sensor is set in a row, is equipped with an optical path below each described color sensor, longitudinally disposed three of every optical routing
Optical imagery eyeglass composition.
3. PCR amplification detector according to claim 1, which is characterized in that be equipped with the second radiator, institute in the shell
The pedestal for stating the second radiator is closely connect with the Peltier.
4. PCR amplification detector according to claim 3, which is characterized in that be equipped with heat dissipation below second radiator
Fan, the housing bottom are equipped with heat dissipating grid, and the heat dissipating grid is located at the lower section of the radiator fan.
5. PCR amplification detector according to claim 1, which is characterized in that the material of first radiator is aluminium.
6. PCR amplification detector according to claim 3, which is characterized in that the material of second radiator is aluminium.
7. PCR amplification detector according to claim 1, which is characterized in that the high temperature air area, target upper temperatures air
Area and the Cryogenic air area, target low air zone are respectively arranged on the opposite two sides of the PCR pipe matrix.
8. PCR amplification detector according to claim 2, which is characterized in that three longitudinally disposed optical imageries
Eyeglass is followed successively by the first plano-convex lens, the second plano-convex lens and third plano-convex lens from top to bottom, wherein the first plano-convex lens,
The plane of second plano-convex lens and third plano-convex lens is all arranged in parallel with the circuit board, and first plano-convex lens are close to institute
The one side for stating circuit board is plane, and second plano-convex lens and third plano-convex lens close to the circuit board are all convex on one side
Face.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910322425.7A CN110117534A (en) | 2019-04-19 | 2019-04-19 | A kind of PCR amplification detector |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910322425.7A CN110117534A (en) | 2019-04-19 | 2019-04-19 | A kind of PCR amplification detector |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110117534A true CN110117534A (en) | 2019-08-13 |
Family
ID=67521252
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910322425.7A Pending CN110117534A (en) | 2019-04-19 | 2019-04-19 | A kind of PCR amplification detector |
Country Status (1)
| Country | Link |
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| CN (1) | CN110117534A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111269823A (en) * | 2020-03-10 | 2020-06-12 | 杭州博日科技有限公司 | Thermal cycling device and PCR instrument |
| CN115074217A (en) * | 2022-07-22 | 2022-09-20 | 南京溯远基因科技有限公司 | PCR amplification instrument with quick wind field heating mechanism |
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| US20060024816A1 (en) * | 2004-06-23 | 2006-02-02 | Applera Corporation | Gas thermal cycler |
| US20070070359A1 (en) * | 2005-09-16 | 2007-03-29 | Hon Hai Precision Ind. Co., Ltd. | Heating geometric measure equipment |
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| CN111269823A (en) * | 2020-03-10 | 2020-06-12 | 杭州博日科技有限公司 | Thermal cycling device and PCR instrument |
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| CN115074217A (en) * | 2022-07-22 | 2022-09-20 | 南京溯远基因科技有限公司 | PCR amplification instrument with quick wind field heating mechanism |
| CN115074217B (en) * | 2022-07-22 | 2023-08-22 | 南京溯远基因科技有限公司 | PCR amplification instrument with rapid wind field heating mechanism |
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Application publication date: 20190813 |