CN115074217B - PCR amplification instrument with rapid wind field heating mechanism - Google Patents

PCR amplification instrument with rapid wind field heating mechanism Download PDF

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Publication number
CN115074217B
CN115074217B CN202210865910.0A CN202210865910A CN115074217B CN 115074217 B CN115074217 B CN 115074217B CN 202210865910 A CN202210865910 A CN 202210865910A CN 115074217 B CN115074217 B CN 115074217B
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shell
plate
heating
pcr amplification
plates
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CN115074217A (en
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陈功俊
朱纪军
陈�峰
施琦
赵晓芳
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Nanjing Superyears Gene Technology Co ltd
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Nanjing Superyears Gene Technology Co ltd
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00Heating or cooling apparatus; Heat insulating devices
    • B01L7/52Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/18Means for temperature control
    • B01L2300/1838Means for temperature control using fluid heat transfer medium

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Air-Flow Control Members (AREA)
  • Cooling Or The Like Of Electrical Apparatus (AREA)

Abstract

The invention discloses a PCR (polymerase chain reaction) amplification instrument with a rapid wind field heating mechanism, and relates to the technical field of PCR amplification instruments. The invention comprises a shell, wherein a storage plate and a baffle plate positioned below the storage plate are connected in the shell, and ventilation openings are formed at two ends of the baffle plate; a fan mechanism is connected in the shell; the bottom wall of the shell is connected with two side plates, and two opposite side surfaces of each side plate are arc-shaped surfaces; mounting plates are uniformly distributed between the two side plates from top to bottom, and the mounting plates are connected with heating assemblies; the casing rotation is connected two pivots, and the pivot is connected with the air flap, and one side that the air flap kept away from the pivot and arcwall face adaptation, through the pivot rotation, adjusts the air flap and corresponds with the mounting panel position for the air current flows through heating element. According to the invention, through the rotation of the two air door plates and the cooperation of the mounting plates with different heights, the airflow in the shell flows through the corresponding heating assembly, so that the airflow is rapidly heated to the corresponding target temperature, and the problems of low heating efficiency and low temperature control accuracy in the prior art are solved.

Description

PCR amplification instrument with rapid wind field heating mechanism
Technical Field
The invention belongs to the technical field of PCR amplification instruments, and particularly relates to a PCR amplification instrument with a rapid wind field heating mechanism.
Background
The temperature change of the PCR amplification instrument in the prior art is realized by a semiconductor refrigerating sheet and a heat conducting module, the temperature is directly controlled by a PID temperature control mode after the temperature is acquired by a temperature measuring probe in the heat conducting module, the temperature of the solution in the PCR tube has hysteresis, and the temperature of the solution in the PCR tube is smaller as the temperature of the solution in the PCR tube approaches the temperature difference between the inside and outside of the target temperature, the temperature is gradually increased, so that the time required for completing PCR amplification is prolonged, the PCR amplification efficiency is low, and the accuracy of an experimental result is influenced.
Meanwhile, the heating temperature is required to be regulated in different reaction stages of PCR, the heating temperature is continuously regulated back and forth between rising and reducing cooling, the temperature is gradually increased due to the fact that the temperature is raised, the temperature is gradually reduced due to the fact that the temperature is lowered, and in the processes of raising and lowering the temperature, the temperature is unstable and overshoot phenomenon exists, and therefore the temperature is difficult to accurately control to just reach the required proper temperature, and the heating efficiency and the accuracy of temperature control are low.
Disclosure of Invention
The invention aims to provide a PCR amplification instrument with a rapid wind field heating mechanism, which ensures that air flow in a shell flows through a corresponding heating assembly through the cooperation of rotation of two air door plates and mounting plates with different heights, so that the air flow in the shell is rapidly heated to a corresponding target temperature, and the problems of low heating efficiency and low temperature control accuracy in the prior art are solved.
In order to solve the technical problems, the invention is realized by the following technical scheme:
the invention relates to a PCR amplification instrument with a rapid wind field heating mechanism, which comprises a shell, wherein a placement opening is formed in the top of the shell, a placement plate positioned below the placement opening is fixedly connected between two inner side walls of the shell, and a plurality of placement holes for placing PCR tubes are formed in the placement plate; the inner wall of the shell is fixedly connected with a baffle plate positioned below the object placing plate, and both ends of the baffle plate are provided with ventilation openings; the inner wall of the shell is rotationally connected with a fan mechanism, and the fan mechanism corresponds to one ventilation opening in position; the bottom wall of the shell is fixedly connected with two side plates which are arranged side by side, and the side surfaces of the two side plates are respectively attached to the two side walls of the shell; the two opposite side surfaces of the side plate are arc-shaped surfaces; a plurality of mounting plates are uniformly distributed between the two side plates from top to bottom, and the upper surfaces of the mounting plates are fixedly connected with heating assemblies; the shell is rotationally connected with two rotating shafts which are symmetrically arranged relative to the side plates, and the rotation centers of the two rotating shafts are concentrically arranged with the circle center of the arc-shaped surface; one end of the rotating shaft penetrates through the side wall of the shell and is fixedly connected with a motor; the pivot fixedly connected with air door board, air door board both sides are laminated with two inside walls of casing respectively, and one side that the air door board kept away from the pivot and arcwall face adaptation for rotate through the pivot, adjust air door board and mounting panel position correspondence, make the air current flow through heating element.
As a preferable technical scheme of the invention, the outer wall of the rotating shaft is coated with a rubber layer, and the rubber layer is attached to the inner wall of the shell.
As a preferable technical scheme of the invention, vent holes which are in one-to-one correspondence are arranged on one side of the shell and one side of the side plate in an array manner; the vent is located between the bottom wall of the housing and the lowermost mounting plate.
As a preferable technical scheme of the invention, a diaphragm plate is fixedly connected between the two side plates, and the upper surface and the lower surface of the diaphragm plate are respectively attached to the mounting plate and the bottom wall of the shell and are used for separating the vent hole array into two groups.
As a preferable technical scheme of the invention, the inner side surface of the side plate is fixedly connected with two fans, and the two fans are respectively corresponding to the positions of the two groups of vent hole arrays.
As a preferable technical scheme of the invention, the heating power of the heating assemblies is increased from top to bottom.
As a preferable technical scheme of the invention, the vertical height of the rotating shaft is larger than the height of the uppermost mounting plate between the two side plates.
As a preferable technical scheme of the invention, the upper surface of the air door plate is in a curved surface structure.
The invention has the following beneficial effects:
according to the invention, the plurality of mounting plates are uniformly distributed between the two side plates in the shell from top to bottom, the upper surfaces of the plurality of mounting plates are fixedly connected with the heating assemblies, and according to the required target temperature, the two air door plates are utilized to rotate to be matched with the mounting plates with different heights, so that the air flow is guided to the heating assemblies corresponding to the heating temperature, the air flow in the shell can be heated to the target temperature more rapidly, and the overall heating efficiency and the accuracy of the heating temperature are effectively improved.
Of course, it is not necessary for any one product to practice the invention to achieve all of the advantages set forth above at the same time.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed for the description of the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of a PCR amplification unit with a rapid wind-field heating mechanism according to the present invention;
FIG. 2 is a schematic view of the rear view structure of FIG. 1;
FIG. 3 is a top plan view of the structure of FIG. 1;
FIG. 4 is a schematic view of the internal structure of the housing;
FIG. 5 is a cross-sectional view taken at A-A of FIG. 3;
FIG. 6 is a schematic view of a position structure of the damper panel after adjustment;
FIG. 7 is a schematic view showing a position structure of an air door plate when a heating temperature is maximum;
FIG. 8 is a schematic view of a position structure of a damper plate during heat dissipation;
in the drawings, the list of components represented by the various numbers is as follows:
the device comprises a 1-shell, a 2-fan mechanism, a 3-side plate, a 4-rotating shaft, a 101-object placing opening, a 102-object placing plate, a 103-object placing hole, a 104-partition plate, a 105-ventilation opening, a 106-ventilation opening, a 301-mounting plate, a 302-heating component, a 303-diaphragm plate, a 401-air door plate and a 402-motor.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
In the description of the present invention, it should be understood that the terms "open," "upper," "lower," "thickness," "top," "middle," "length," "inner," "peripheral," and the like indicate orientation or positional relationships, merely for convenience in describing the present invention and to simplify the description, and do not indicate or imply that the components or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the present invention.
Example 1
Referring to fig. 1-3, the invention discloses a PCR amplification instrument with a rapid wind field heating mechanism, which comprises a housing 1, wherein an object placing opening 101 is formed in the top of the housing 1, and a cover plate for sealing the object placing opening 101 is hinged to the housing 1.
The two inner side walls of the shell 1 are fixedly connected with a storage plate 102 positioned below the storage opening 101, the length of the storage plate 102 is smaller than that of the shell 1, the storage plate 102 is provided with a plurality of placement holes 103 for placing PCR tubes, and when the PCR tubes are inserted into the placement holes 103 and the cover plate is covered.
The inner wall of the shell 1 is fixedly connected with a baffle 104 positioned below the storage plate 102, the baffle 104 is attached to the four inner side walls of the shell 1, the baffle 104 can be fixed by welding, gluing or screw connection, and ventilation openings 105 are formed in two ends of the baffle 104. The inner wall of the shell 1 is rotationally connected with a fan mechanism 2, the fan mechanism 2 comprises an air duct and a driving motor, the driving motor is installed on the outer wall of the shell 1, an output shaft of the driving motor is connected with the air duct and used for driving the air duct to rotate, and the air duct of the fan mechanism 2 corresponds to the position of one of the ventilation openings 105.
As shown in fig. 4 and 5, the wind tunnel of the fan mechanism 2 corresponds to the position of the vent 105 on the right side of the partition 104 and is located above the vent 105, so that the air flow under the partition 104 enters the upper side of the partition 104 through the vent 105 on the right side by the wind tunnel rotation and returns to the lower side of the partition 104 through the vent 105 on the left side.
The bottom wall of the shell 1 is fixedly connected with two side plates 3 which are arranged side by side, and the side surfaces of the two side plates 3 are respectively attached to the two side walls of the shell 1; the two opposite side surfaces of the side plate 3 are arc-shaped surfaces; three mounting plates 301 are uniformly distributed between the two side plates 3 from top to bottom, heating assemblies 302 are fixedly connected to the upper surfaces of the three mounting plates 301, the heating assemblies 302 can be resistance wire heating assemblies, the heating power of the three heating assemblies 302 is sequentially increased from top to bottom, and the target temperature to be heated is also sequentially increased.
The rotation of the shell 1 is connected with two rotating shafts 4 which are symmetrically arranged relative to the side plates 3, the rotation centers of the two rotating shafts 4 are concentric with the circle center of the arc-shaped surface, one end of each rotating shaft 4 penetrates through the side wall of the shell 1 and is fixedly connected with a motor 402, and the motor 402 is used for driving the rotating shafts 4 to rotate.
Wherein, the outer wall cladding of pivot 4 has the rubber layer, and the rubber layer is laminated with casing 1 inner wall to avoid the air current to leak by between pivot 4 and the casing 1 inner wall. The rotating shaft 4 is fixedly connected with the air door plate 401, two sides of the air door plate 401 are respectively attached to two inner side walls of the shell 1, one side, far away from the rotating shaft 4, of the air door plate 401 is matched with the arc-shaped surface of the side plate 3 and used for rotating through the rotating shaft 4, and the air door plate 401 is adjusted to correspond to the mounting plate 301 in position, so that air flows through the heating assembly 302.
The air door plate 401 side is attached to the inner side wall of the shell 1, so that air flow can be effectively prevented from leaking to the lower side of the air door plate 401, the air flow can only flow along the upper surface of the air door plate 401, meanwhile, the air door plate 401 is matched with the arc-shaped surface of the side plate 3, and the air door plate 401 and the side plate 3 keep good sealing effect, so that the air flow flowing along the upper surface of the air door plate 401 flows through the upper surface of the mounting plate 301.
As shown in fig. 4 and 5, when the interior of the casing 1 needs to be heated, the two damper plates 401 are rotated to be flush with the uppermost mounting plate 301, and after the blower mechanism 2 is started, the airflow flowing direction in the interior of the casing 1 is shown by an arrow in the figure, and the airflow heated by the heating component 302 enters above the partition 104, so that the PCR tube on the opposite plate 102 is heated.
As shown in fig. 6, when the heating temperature needs to be increased, the two damper plates 401 are rotated to be flush with the mounting plate 301 of the second layer, and the heating is performed by the heating component 302 with higher power, so that the heating component 302 with the corresponding heating temperature is used for heating, and the heated temperature of the air flow in the housing 1 is more accurate.
As shown in fig. 7, similarly, when the heating temperature is required to be increased, the two damper plates 401 are rotated to be flush with the lowest mounting plate 301, and the heating is performed by the heating unit 302 on the lowest mounting plate 301.
In addition, when heating is performed by the heating element 302 on the second-layer mounting board 301, the heating element 302 on the second-layer mounting board 301 may be used alone, or the heating elements 302 on the uppermost layer and the second layer may be turned on at the same time, so that the heating efficiency can be improved.
Similarly, when the lowest heating element 302 is used for heating, the lowest heating element 302 may be used alone for heating, or the highest heating element 302 and the lowest heating element 302 may be turned on simultaneously, or the second heating element 302 and the lowest heating element 302 may be turned on simultaneously, or all the heating elements 302 may be turned on simultaneously for heating, thereby improving the overall heating efficiency.
When the heating device is used, the heating assemblies 302 with the corresponding layers are selected to heat according to the required target temperature, so that the heating temperature is accurate, and meanwhile, the temperature of each layer of heating assembly 302 can be independently fine-tuned, so that the whole heating range is larger, and the accuracy is higher.
And, through utilizing air door board 401 and mounting panel 301 cooperation for the air current forms the inner loop in casing 1 inside, thereby makes the heating efficiency of air current obtain great improvement, simultaneously, low-power heating element 302 is located the upper strata, and at this moment, after air door board 401 and the mounting panel 301 cooperation on the upper strata, makes the air current inner loop space minimum, thereby does benefit to the temperature and reaches target temperature fast.
When a higher temperature is needed, the air door plate 401 is matched with the mounting plate 301 at the lower layer, so that the internal circulation space of the air flow is increased, the internal air flow is prevented from being heated instantly, the temperature overshoot phenomenon is easy to occur, and the overall heating precision is improved.
The vertical direction of the rotating shaft 4 is higher than the uppermost mounting plate 301 between the two side plates 3, and the upper surface of the air door plate 401 is in a curved surface structure, so that the air door plate 401 is inclined after the air door plate 401 is matched with the mounting plate 301, and the air flow can be guided better.
Example two
On the basis of the first embodiment, as shown in fig. 2 and 5, vent holes 106 are formed in an array on one side of the housing 1 and one side of the side plate 3, and the vent holes 106 are located between the bottom wall of the housing 1 and the lowest mounting plate 301.
When heat dissipation is required inside the housing 1, the air door plate 401 is rotated to a position lower than the lowest mounting plate 301, so that air flow enters between the lowest mounting plate 301 and the bottom wall of the housing 1, and the air flow inside the housing 1 is exhausted through the ventilation holes 106.
Meanwhile, a diaphragm 303 is fixedly connected between the two side plates 3, and the upper surface and the lower surface of the diaphragm 303 are respectively attached to the mounting plate 301 and the bottom wall of the shell 1 for separating the vent hole 106 array into two groups. The inner side surface of the side plate 3 is fixedly connected with two fans by screws and the like, and the two fans respectively correspond to the array positions of the two groups of vent holes 106.
As shown in fig. 8, the air is partitioned by a diaphragm 303 so that the right fan can inject outside air into the housing 1 through the right vent hole 106 when radiating heat, as indicated by a broken arrow in the figure. And the left fan can rapidly exhaust the hot air in the shell 1 through the left vent hole 106, so that the overall heat dissipation and cooling efficiency is greatly improved.
Example III
The heating control method of the PCR amplification instrument with the rapid wind field heating mechanism specifically comprises the following steps:
step one: when the heating is required to a lower temperature, as shown in fig. 5, the two damper plates 401 are rotated to be flush with the uppermost mounting plate 301, after the blower mechanism 2 is started, the airflow flowing direction inside the casing 1 is shown by an arrow in the figure, and the airflow heated by the heating component 302 enters the upper part of the partition 104, so that the PCR tube on the opposite plate 102 is heated.
At this time, after the damper plate 401 is matched with the mounting plate 301 at the uppermost layer, the internal circulation space of the air flow is minimized, so that the temperature can reach the target temperature quickly.
Step two: when it is desired to raise the heating temperature, as shown in fig. 6, the two damper plates 401 are rotated to be flush with the mounting plate 301 of the second layer, and heated by the heating unit 302 having a larger power, thereby heating by the heating unit 302 having a corresponding heating temperature.
At this time, the heating elements 302 on the second-layer mounting board 301 may be used alone to heat the air flow direction as shown by the arrows in the figure, or the heating elements 302 on the uppermost layer and the second-layer may be turned on simultaneously, so that the heating efficiency may be improved, and the heating element 302 on the second layer may heat a higher temperature than the heating element 302 on the uppermost layer, so that the required heating temperature may be precisely controlled by adjusting the set temperature of the heating element 302 on the second layer.
Step three: when a higher heating temperature is required, as shown in fig. 7, the two damper plates 401 are rotated to be flush with the lowest mounting plate 301, and heated by the heating unit 302 on the lowest mounting plate 301.
At this time, when the heating elements 302 at the lowest layer are used alone to heat, the heating elements 302 at the highest layer and the heating elements 302 at the lowest layer may be turned on simultaneously, or the heating elements 302 at the second layer and the heating elements 302 at the lowest layer may be turned on simultaneously, or all the heating elements 302 may be turned on simultaneously to heat, thereby improving the overall heating efficiency.
The heating temperature of the heating element 302 at the lowest layer is the highest, so that the required heating temperature can be precisely controlled only by adjusting the set temperature of the heating element 302 at the lowest layer, thereby improving the heating efficiency and simultaneously effectively improving the accuracy of the heating temperature.
Step four: as shown in fig. 8, when heat dissipation and cooling are required, the two damper plates 401 are rotated to a position lower than the lowest mounting plate 301, so that air flow enters between the lowest mounting plate 301 and the bottom wall of the housing 1, and the hot air flow is discharged through the ventilation holes 106.
The direction of the air flow is shown by the arrow in the figure, and in order to improve the heat dissipation efficiency, the fan on the left side of the diaphragm 303 may be turned on, so that the hot air flow is discharged through the air vent 106 on the left side.
Meanwhile, the right fan can be started to inject external cold air into the shell 1 to participate in air circulation, and the direction of the cold air is shown by a broken line arrow in the figure, so that the cooling speed is further improved, and the overall temperature regulation efficiency is improved.
In the description of the present specification, the descriptions of the terms "one embodiment," "example," "specific example," and the like, mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present invention. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiments or examples. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The preferred embodiments of the invention disclosed above are intended only to assist in the explanation of the invention. The preferred embodiments are not exhaustive or to limit the invention to the precise form disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best understand and utilize the invention. The invention is limited only by the claims and the full scope and equivalents thereof.

Claims (8)

1. The utility model provides a PCR amplification instrument with quick wind field heating mechanism, includes casing (1), thing mouth (101) have been seted up at casing (1) top, its characterized in that:
a storage plate (102) positioned below the storage opening (101) is fixedly connected between the two inner side walls of the shell (1), and a plurality of placement holes (103) for placing the PCR tubes are formed in the storage plate (102);
the inner wall of the shell (1) is fixedly connected with a partition plate (104) positioned below the object placing plate (102), and ventilation openings (105) are formed in two ends of the partition plate (104); the inner wall of the shell (1) is rotationally connected with a fan mechanism (2), and the fan mechanism (2) corresponds to one ventilation opening (105);
the bottom wall of the shell (1) is fixedly connected with two side plates (3) which are arranged side by side, and the side surfaces of the two side plates (3) are respectively attached to the two side walls of the shell (1); the two opposite side surfaces of the side plate (3) are arc-shaped surfaces; a plurality of mounting plates (301) are uniformly distributed between the two side plates (3) from top to bottom, and the upper surfaces of the plurality of mounting plates (301) are fixedly connected with heating assemblies (302);
the shell (1) is rotationally connected with rotating shafts (4) symmetrically arranged on the two opposite side plates (3), and the rotation centers of the two rotating shafts (4) are concentrically arranged with the circle center of the arc-shaped surface; one end of the rotating shaft (4) penetrates through the side wall of the shell (1) and is fixedly connected with a motor (402);
the rotating shaft (4) is fixedly connected with the air door plate (401), two sides of the air door plate (401) are respectively attached to two inner side walls of the shell (1), one side, far away from the rotating shaft (4), of the air door plate (401) is matched with the arc-shaped surface and used for rotating through the rotating shaft (4), the position of the air door plate (401) is adjusted to correspond to that of the mounting plate (301), and air flows through the heating assembly (302).
2. The PCR amplification apparatus with the rapid wind field heating mechanism according to claim 1, wherein a rubber layer is coated on the outer wall of the rotating shaft (4), and the rubber layer is attached to the inner wall of the shell (1).
3. The PCR amplification apparatus with the rapid wind field heating mechanism according to claim 1, wherein vent holes (106) are formed in one-to-one correspondence on one side of each of the shell (1) and the side plate (3); the vent hole (106) is positioned between the bottom wall of the shell (1) and the lowest mounting plate (301).
4. A PCR amplification instrument with a rapid wind field heating mechanism according to claim 3, wherein a diaphragm (303) is fixedly connected between the two side plates (3), and the upper surface and the lower surface of the diaphragm (303) are respectively attached to the mounting plate (301) and the bottom wall of the housing (1) for separating the array of ventilation holes (106) into two groups.
5. The PCR amplification apparatus with the rapid wind field heating mechanism according to claim 4, wherein two fans are fixedly connected to the inner side surface of the side plate (3), and the two fans correspond to the array positions of the two groups of ventilation holes (106) respectively.
6. The PCR amplification instrument with rapid wind-field heating mechanism as set forth in claim 1, wherein the heating power of the plurality of heating elements (302) increases sequentially from top to bottom.
7. The PCR amplification instrument with the rapid wind field heating mechanism as claimed in claim 1, wherein the vertical height of the rotating shaft (4) is larger than the uppermost mounting plate (301) between the two side plates (3).
8. The PCR amplification instrument with rapid wind-field heating mechanism as set forth in claim 1, wherein the upper surface of the damper plate (401) is curved.
CN202210865910.0A 2022-07-22 2022-07-22 PCR amplification instrument with rapid wind field heating mechanism Active CN115074217B (en)

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CN115074217B true CN115074217B (en) 2023-08-22

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Citations (3)

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Publication number Priority date Publication date Assignee Title
CN203333663U (en) * 2013-02-28 2013-12-11 王逸斐 High-speed wind field PCR (Polymerase Chain Reaction) instrument
CN106119426A (en) * 2016-06-24 2016-11-16 南京沃凯生物工程有限公司 Rapid PCR amplification instrument temperature control method
CN110117534A (en) * 2019-04-19 2019-08-13 广州小飞虎电子科技有限公司 A kind of PCR amplification detector

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US20120034688A1 (en) * 2010-08-04 2012-02-09 Griffin Stephen E True nucleic acid amplification
WO2018139788A1 (en) * 2017-01-25 2018-08-02 주식회사 유진셀 Apparatus for high-speed nucleic acid amplification and method for temperature control of nucleic acid amplification reaction

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Publication number Priority date Publication date Assignee Title
CN203333663U (en) * 2013-02-28 2013-12-11 王逸斐 High-speed wind field PCR (Polymerase Chain Reaction) instrument
CN106119426A (en) * 2016-06-24 2016-11-16 南京沃凯生物工程有限公司 Rapid PCR amplification instrument temperature control method
CN110117534A (en) * 2019-04-19 2019-08-13 广州小飞虎电子科技有限公司 A kind of PCR amplification detector

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一种新型PCR扩增仪的温控系统;袁跃峰, 马文丽, 陈虎, 郑文岭, 梁斌;上海大学学报(自然科学版)(第05期);第102-106页 *

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