CN110115697A - The composition of prevention and treatment scytitis containing cordyceps sinensis - Google Patents
The composition of prevention and treatment scytitis containing cordyceps sinensis Download PDFInfo
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- CN110115697A CN110115697A CN201810122515.7A CN201810122515A CN110115697A CN 110115697 A CN110115697 A CN 110115697A CN 201810122515 A CN201810122515 A CN 201810122515A CN 110115697 A CN110115697 A CN 110115697A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
- A61K31/357—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having two or more oxygen atoms in the same ring, e.g. crown ethers, guanadrel
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
- A61K31/365—Lactones
- A61K31/366—Lactones having six-membered rings, e.g. delta-lactones
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7028—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages
- A61K31/7034—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages attached to a carbocyclic compound, e.g. phloridzin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/02—Algae
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/062—Ascomycota
- A61K36/066—Clavicipitaceae
- A61K36/068—Cordyceps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/282—Artemisia, e.g. wormwood or sagebrush
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/4973—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom
- A61K8/498—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom having 6-membered rings or their condensed derivatives, e.g. coumarin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
- A61K8/602—Glycosides, e.g. rutin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/10—General cosmetic use
Abstract
The present invention relates to the compositions of the prevention and treatment scytitis containing cordyceps sinensis, belong to drug, cosmetic field.Technical problem to be solved by the invention is to provide the compositions of prevention and treatment scytitis, the composition includes the raw material of following weight proportion: dihydroartemisinine, Artesunate, qinghaosu or 0.001~1.0 part of 0.10~5.00 part of 0.05~10 part of Artemisia annua extract, brown algae extract, the cordyceps sinensis containing qinghaosu, wherein, the content of phytosterol is 30~40ppm in the brown algae extract.The experimental results showed that, the present composition has the function of significantly inhibiting scytitis, effect is better than with purslane extract, bisabolol, dipotassium glycyrrhizinate etc. being the cosmetics of active constituent, and, also it is avoided that glucocorticoid medicine using bring side effect, has a vast market foreground.
Description
Technical field
The present invention relates to the compositions of the prevention and treatment scytitis containing cordyceps sinensis, belong to drug, cosmetic field.
Background technique
Keratoderma is the compact texture being staggeredly made of the stacking of abiotic keratinocyte, the barrier of cuticula
Function is exactly to maintain epidermis normal osmosis barrier, can effectively prevent physical and chemical, biological stimulus, effectively prevent the warp of body
Transepidermal water and nutrients are lost.If stratum corneum barrier function is lost, non-visible dehydration can increase by 10 times or more, nutrients
Matter, electrolyte are easily lost, and external microorganism is easy to invade, very harmful to skin health.
Since current environmental pollution causes air quality to change, office environment, bad cosmetics injure (especially illegal
Add the product of glucocorticoids), the factors such as drug abuse (not using under physician guidance), skin health is by serious prestige
The side of body.Wherein, skin sensitivity is even more the main problem for perplexing numerous female friends.Due to skin sensitivity, skin vulnerable to environmental stimuli,
Environmental factor (UV, cold and hot temperature, wind, dry air, pollution), chemical substance (cleaning supplies, soap), mechanical pressure (are scraped recklessly
Repair hair, clothes), the influence of the state of mind (pressure, emotion) causes skin " allergy " symptom occur, is seriously affecting women just
Often life causes the injury of psychology and body.Ratio of the sensitive skin in crowd is made us in Europe and the U.S. about 40%
Cause anxiety.By showing sensibility skin through the test of epidermis dehydration (TEWL) to sensibility skin, there are serious skin keratins
The case where layer barrier function damage.In addition, also usually there is drying, shouting pain, burns, is tight, itch, erythema, mound in sensitive skin
The inflammatory symptoms such as rash, redness.
Currently, there is the treatment method master of the problems such as inflammation for the decline of sensibility skin stratum corneum barrier function, skin
It include: 1, external application glucocorticosteroid hormonal medicaments, such as dexamethasone, hydrocortisone ointment；2, external application tetracycline medication
(Doxycycline, minocycline, fortimicin, Metronidazole Cream)；3, external application non-steroid anti-inflammatory drug (that acid of Indomethacin, fluorine atmosphere
Butyl ester)；4, external application immunosuppressor includes Tacrolimus paste, Elidel ointment；5, using containing purslane extract, red
The cosmetics of the ingredients such as bisabol, dipotassium glycyrrhizinate.However, there is apparent defect in above-mentioned treatment method.Wherein, sugared cortex
Parahormone drug, tetracycline medication, non-steroid anti-inflammatory drug, immunosuppressor etc. belong to drug, are not suitable for daily skin
Maintenance and skin care, moreover, there are very big security risks for the use of above-mentioned external drug, it is necessary under the guidance of physician specialty
It uses.For example, long-term external application glucocorticoid can cause a kind of specific skin inflammation --- steroid dependent dermatitis, it is main to occur
In face, clinical manifestation is often accompanied by telangiectasis and the slight atrophy of skin in addition to the inflammatory reactions such as face red spot, papule.
Cosmetics containing ingredients such as purslane extract, bisabolol, dipotassium glycyrrhizinates have it is certain releive, antiallergic effect, but
Cannot play the role of reparation and skin quality to sensibility skin especially hormonal dependent skin improves, to the inflammation of such skin
Situation cannot play good inhibiting effect.
Therefore, it needs to develop the better effect for preventing and treating scytitis, and the substitute products of few side effects.
Summary of the invention
The purpose of the present invention is to provide the compositions of prevention and treatment scytitis.
The present invention provides the composition of prevention and treatment scytitis, the raw materials comprising following weight proportion: dihydroartemisinine, blueness
Artemisic succinate, qinghaosu or 0.10~5.00 part of 0.05~10 part of Artemisia annua extract, brown algae extract containing qinghaosu, winter
0.001~1.0 part of worm summer grass, wherein the content of phytosterol is 30~40ppm in the brown algae extract.
Further, the composition includes the raw material of following weight proportion: dihydroartemisinine, Artesunate, sweet wormwood
Element or 0.1~2 part of 0.01~1 part of Artemisia annua extract, brown algae extract, cordyceps sinensis 0.005~0.2 containing qinghaosu
Part.
Preferably, the composition includes the raw material of following weight proportion: dihydroartemisinine, Artesunate, qinghaosu
Or 0.1~0.5 part of the Artemisia annua extract containing qinghaosu, 0.5~2 part of brown algae extract, cordyceps sinensis 0.001~0.03
Part.
It is further preferred that the composition include following weight proportion raw material: dihydroartemisinine, Artesunate,
Qinghaosu or 0.02 part of 1~2 part of 0.2~0.5 part of Artemisia annua extract, brown algae extract, cordyceps sinensis containing qinghaosu.
Most preferably, the weight proportion of the raw material are as follows: dihydroartemisinine, Artesunate, qinghaosu contain qinghaosu
Artemisia annua extract: brown algae extract: cordyceps sinensis=0.5:2:0.02 or 0.2:1:0.02.
Further, the brown algae extract is extracted with chloroform, methanol or its mixed solvent.
Further, the brown algae extract is prepared by the following method to obtain: brown algae is taken, with chloroform and methanol
Mixed solvent extract, crude extract is successively with chloroform, chloroform: the mixed solvent of acetone volume ratio 3:1 carries out silica gel column chromatography divide
Grade separation, collect chloroform-acetone eluate to get.
Further, the in the mixed solvent chloroform of the chloroform and methanol: the volume ratio of methanol is 2:1.
Further, crude extract is first eluted with chloroform, then is washed with the chloroform-acetone mixed solvent of 2 times of volumes of eluent
It is de-.
Further, the brown algae extract is to extract to obtain using Phaeodactylum tricornutum as raw material.
Further, the brown algae extract be with the Muriella aurantiaca of Phaeodactylum tricornutum and/or
Ruttnera lamellosa microalgae cell is that raw material extracts to obtain.
Further, in the brown algae extract Muriella aurantiaca microalgae cell extract and
The mass ratio of Ruttnera lamellosa microalgae cell extract is 1:1.
Further, the cordyceps sinensis is section ergot fungus cordyceps sinensis bacterium Cordyceps sinensis
(BerK.) drying complex of Sacc. parasitism stroma on the larvae of batrachidae insects and larva corpse.
Further, the cordyceps sinensis is added in the form of primary powder or extract.
Further, the extract is water extract.
Further, in the extract, polysaccharide >=1mg/L, mannitol >=1mg/L, adenosine >=1mg/L.
Preferably, in the extract, polysaccharide >=500mg/L, mannitol >=700mg/L, adenosine >=5mg/L.
Further, the composition is that acceptable auxiliary material is added using the raw material of each weight proportion as active constituent
Or complementary ingredient, the drug being prepared, cosmetics.
Further, the cosmetics are Essence, lotion, freeze-dried powder, creme, gel, facial mask, foundation cream, muffin, wash one's face
Milk or aqua.
Further, the creme is face cream or eye cream.
Further, the drug is external preparation.
Further, the complementary ingredient is selected from whitening agent, emollient, anti-acne agent, ultraviolet absorbing agent, skin tune
Manage ingredient one or more kinds of in agent.
The present invention provides the preparation method of the composition, include the following steps: the raw material for taking each weight proportion, is added
In drug, cosmetics acceptable auxiliary material or complementary ingredient to get.
The present invention provides purposes of the composition in the drug, cosmetics of preparation prevention and treatment scytitis.
Further, the prevention and treatment scytitis is to alleviate skin irritation, burn, is tight, itch, erythema, papule or red
It is swollen.
The present invention provides use of the composition in the drug, cosmetics of preparation raising keratoderma barrier function
On the way.
Further, the raising keratoderma barrier function is to reduce moisture to be lost through keratoderma.
Wherein, the Artemisia annua extract containing qinghaosu is with qinghaosu poidometer.
Artemisia annua (ARTEMISIAANNUA) extract of the present invention, to its extracting method without limitation, as long as wherein containing
Raising keratoderma barrier function, prevention and treatment scytitis can be played by playing the role of qinghaosu.
Qinghaosu: molecular formula C15H22O5, molecular weight 282.33, constituent content: C 63.81%, H 7.85%, O
28.33%.Colourless acicular crystal, bitter.It is readily soluble in acetone, ethyl acetate, chloroform, benzene and glacial acetic acid, ethyl alcohol and methanol,
It can dissolve in ether and petroleum ether, it is almost insoluble in water.Fusing point: 156-157 DEG C.
The derivative dihydroartemisinine of qinghaosu: Chinese name (3R, 5aS, 6R, 8aS, 9R, 12S, 12aR)-octahydro -3,6,
9- trimethyl -3,12- bridging oxygen -12H- pyrans simultaneously seven ring -10 (3H) -ol molecular formula C15H24O5 of [4,3-j] -1,2- benzo dioxy
Molecular weight 284.34806, white crystals or crystalline powder.
The derivative Artesunate of qinghaosu: molecular formula: C19H28O8, molecular weight: 384.4208, white crystals, fusing point
1290 DEG C -1400 DEG C, odorless, bitter.It is readily soluble in chloroform, it dissolves in acetone, it is slightly molten in methanol or ethyl alcohol, it is several in water
It is insoluble.
Cordyceps sinensis of the present invention is section ergot fungus cordyceps sinensis bacterium Cordyceps sinensis (BerK.)
Sacc. the drying complex of parasitic stroma and larva corpse on the larvae of batrachidae insects, is also possible to aweto
Fermentation mycelium.Wherein, the weight proportion of active constituent is with cordyceps sinensis dry product poidometer.
Cosmetics of the present invention refer to embrocate, spray or other similar method, it is any to intersperse among human body surface
Position (skin, hair, nail, lip etc.), to reach cleaning, eliminate bad smell, skin care, beauty and modification purpose dailyization
Learn industrial products.
The present invention is with the extract containing qinghaosu and its derivative or qinghaosu >=0.1%, brown algae extract and winter
Worm summer grass compounded combination be raw material, be equipped with emulsifier related with cosmetic formulations, assistant for emulsifying agent, skin conditioning agent, whitening agent,
Brightening agent, colorant, moisturizer, solubilizer, surfactant, preservative, aromatic, emollient, anti-acne agent, film forming agent,
Thickener, pH adjusting agent, buffer, stabilizer, ultraviolet absorbing agent etc. are prepared into and sensitive skin, inflammation skin are suitble to use,
With reparation skin damage barrier function, the aqua for preventing and treating scytitis, lotion, Essence, face cream, facial mask, freeze-dried powder, wash one's face
The cosmetics such as milk.Various products of the present invention can be used for human body face (including eye), neck, trick and whole skin.
The present invention provides the compositions of prevention and treatment scytitis.The experimental results showed that the present composition has significantly
Inhibit the effect of scytitis, effect is better than with purslane extract, bisabolol, dipotassium glycyrrhizinate etc. being the change of active constituent
Cosmetic has a vast market foreground it can also avoiding glucocorticoid medicine using bring side effect.
Specific embodiment
Raw material, equipment used in the specific embodiment of the invention are known product, are obtained by purchase commercial product.
Brown algae extract used in following embodiments is specifically with Phaeodactylum tricornutum (PHAEODACTYLUM
TRICORNUTUM it) is extracted for raw material；Content of phytosterol is 30~40ppm in the extract；Purchase is certainly
Greenaltech S.L C/Baldiri i Reixac 408028Barcelona(Spain)；Extracting method are as follows: phaeodactylum tricornutum refers to
Algae two kinds of microalgae cell Muriella aurantiaca and Ruttnera lamellose (wild isolated strains, by
Separation is collected by Greenaltech company), it is freezed dried in -15 DEG C~-10 DEG C, the chloroform for being 2:1 with volume ratio: methanol mixing
Solvent is extracted from dry algae, and crude extract successively carries out silica gel column chromatography point with chloroform, chloroform-acetone (3:1), acetone and ethyl alcohol
Grade separation (is first eluted with chloroform, then is eluted with the chloroform-acetone of 2 times of volumes of eluent).Chloroform-acetone eluant is collected,
Purified to obtain product with HPLC, then the two 1:1 in mass ratio mixing is to get Phaeodactylum tricornutum extract.
Artemisia annua extract used in following embodiments is to extract to obtain by following methods: artemisia annua
(ARTEMISIAANNUA) it is crushed after 40 DEG C of leaf dry 3h, Ye Fenyu petroleum ether 1:5 in mass ratio mixing is heated to 60 DEG C, mentions
It takes 2 times, keeps the temperature 2h every time, merging filtrate is concentrated to give crude extract.Crude extract is rinsed with 4 times of petroleum ethers of its weight, then thick with 20 times
The petroleum ether of extract weight: ethyl acetate=20:1 solution rinses, the rear petroleum ether with 20 times of crude extract weight: ethyl acetate
The solution that the solution of=1:4 rinses rinses.By 90% alcohol crystal, after filtering, the crystalline solid of Artemisia annua extract is obtained.Crystallization
Body: glycerol three (thylhexoic acid) ester: stearine: PEG-100 stearate=1:10-100:10-100:10-100 is molten
Solve Artemisia annua extract.
Cordyceps extract used in following embodiments is prepared by the following method to obtain:
1, it cleans:
Acquire high quality cordyceps sinensis (section ergot fungus cordyceps sinensis bacterium Cordyceps sinensis (BerK.)
Sacc. the drying complex of parasitic stroma and larva corpse on the larvae of batrachidae insects), winter worm summer wash with distilled water
Grass removes cordyceps sinensis surface silt impurity.
2, dry:
Cordyceps sinensis after cleaning controls moisture content within 10% in 15 DEG C or less low temperature dryings.
3, it crushes:
Cordyceps sinensis is crushed.
4, it extracts:
4.1 extract proportion scale:
| Raw material | Mass percent (wt%) |
| Cordyceps sinensis powder | 1% |
| Distilled water | 99% |
Cordyceps sinensis powder water mixed solution is heated to 30-35 DEG C by 4.2, opens ultrasonic wave 25-30KHZ assisted extraction 20-
25min obtains cordyceps sinensis crude extract.
5, it filters:
Obtained cordyceps sinensis crude extract is filtered with miillpore filter (0.2-0.4 μm of aperture), it is miscellaneous to remove solid
Matter, resulting filtrate are required cordyceps extracting liquid.
In following embodiment, using cordyceps sinensis: water is the extracting solution of 1:99, wherein containing amino acid:
512mg/L, polysaccharide: 555mg/L, mannitol: 762mg/L, adenosine: 5.3mg/L.
The preparation of the Essence of the present invention of embodiment 1
Preparation method:
1, it is cooled to 60-70 DEG C of addition carbomer after accurately weighing water ebuillition of heated, stirring and dissolving is uniform；
2, glycerol, butanediol, artemisia annua (ARTEMISIAANNUA) extract, ceramide-III is accurately weighed to be heated to
70 DEG C make it completely dissolved；
3, the carbomer in confirmation 1 is completely dissolved in uniform situation, is slowly added into Sodium Hyaluronate under stiring, is made it
It is completely dissolved, is sufficiently swollen；
4, precise PEG-75 sher butter glyceride type, double-PEG-18 methyl ether dimethylsilanes are added in 1 and stir
Uniformly；
5, it is added with stirring sodium hydroxide solution neutralization, and tests pH value, determines pH range in 5.0-7.0；
6,40 DEG C are cooled to hereinafter, stirring evenly in 2 additions 5；
By precise and the Cordyceps extract mixed, Phaeodactylum tricornutum extract, Phenoxyethanol, essence and
Cremophor RH40 is added in 6, stirs evenly discharging.
The preparation of the toner of the present invention of embodiment 2
Preparation method:
1,60-70 DEG C is cooled to after accurately weighing water ebuillition of heated；
2, glycerol, butanediol, glycerin polyether -26, artemisia annua (ARTEMISIAANNUA) extract, neural acyl are accurately weighed
Amine-III is heated to 70 DEG C and makes it completely dissolved；
3, precise PEG-75 sher butter glyceride type is added in 1 and stirs evenly；
4,40 DEG C are cooled to hereinafter, stirring evenly in 2 additions 3；
5, by precise and the Phaeodactylum tricornutum extract mixed, Phenoxyethanol, essence and PEG-40 hydrogenated castor
Oil is added in 4, stirs evenly discharging.
The preparation of the mask of the present invention of embodiment 3
Preparation method:
1,60-70 DEG C of addition card wave is cooled to after accurately weighing water, glycerol, butanediol, -26 ebuillition of heated of glycerin polyether
Nurse, stirring and dissolving are uniform；
2, the carbomer in confirmation 1 is completely dissolved in uniform situation, is slowly added into Sodium Hyaluronate under stiring, is made it
It is completely dissolved, is sufficiently swollen；
3, glycerol, butanediol, glycerin polyether -26, artemisia annua (ARTEMISIAANNUA) extract, neural acyl are accurately weighed
Amine-III is heated to 70 DEG C and makes it completely dissolved；
4, precise PEG-75 sher butter glyceride type is added in 2 and stirs evenly；
5, it is added with stirring sodium hydroxide solution neutralization, and tests pH value, determines pH range in 5.0-7.0；
6, it is cooled to 40 DEG C to be added in 4 hereinafter, 3, trehalose, biological carbohydrate gum -1, silandiol salicylate is added, stirring
Uniformly；
7, by precise and the Cordyceps extract mixed, Phaeodactylum tricornutum extract, Phenoxyethanol, caprylic
Acid esters, decoyl hydroximic acid, 1,2- pentanediol, essence and Cremophor RH40 are added in 6, stir evenly discharging.
The preparation of the lotion of the present invention of embodiment 4
Preparation method:
1, C12-20 alkyl glucoside, stearine, C14-22 alcohol, PEG-100 stearate, poly- two are accurately weighed
Methylsiloxane, artemisia annua (ARTEMISIAANNUA) extract, ceramide-III, glycerol three (thylhexoic acid) ester, octanoic acid/
Triglyceride DDD, jojoba seed oil are stand-by in oily phase pot, being heated to 80-85 DEG C；
2, water, glycerol, butanediol, 1,2- pentanediol, carbomer are accurately weighed in water phase pot, stirring keeps material completely molten
Solution, is heated to 80-85 DEG C；
3, oily phase pot material is added in water phase pot under stiring, homogeneous 5min, cools to 45 after oil phase material adds
DEG C, addition Cordyceps extract, Phaeodactylum tricornutum extract, Phenoxyethanol, methyl hydroxybenzoate, Nipasol, essence stirring are mixed
It closes uniformly, sodium hydroxide (10%) is added and neutralizes, and tests pH value, determine that pH range in 5.0-7.0, stirs 10min, discharging.
The preparation of the face cream of the present invention of embodiment 5
Preparation method:
1, C20-22 alcohol phosphate, stearine, C20-22 alcohol, PEG-100 stearate, poly- diformazan are accurately weighed
Radical siloxane, glycerol three (thylhexoic acid) ester, caprylic/capric triglyceride, jojoba seed oil, artemisia annua
(ARTEMISIAANNUA) extract, ceramide-III, Butyrospermum parkii fruit rouge are stand-by in oily phase pot, being heated to 80-85 DEG C；
2, water, glycerol, dipropylene glycol, 1,2- pentanediol, carbomer are accurately weighed in water phase pot, stirring keeps material complete
Dissolution, is heated to 80-85 DEG C；
3, oily phase pot material is added in water phase pot under stiring, homogeneous 5min, cools to 45 after oil phase material adds
DEG C, Cordyceps extract, Phaeodactylum tricornutum extract, Phenoxyethanol, methyl hydroxybenzoate, Nipasol, flowers and plants essence is added and stirs
Mix uniformly mixed, addition sodium hydroxide (10%), stirring 10min；
4, cooling, it is cooled to room temperature discharging.
Beneficial effects of the present invention are proved below by way of test example.
1 Artemisia annua extract of test example and qinghaosu alleviate skin irritation, burn, is tight, itch, erythema, papule, redness
The effect of
1. experiment purpose: investigating lotion of the present invention (its active constituent includes qinghaosu) and alleviate skin irritation, burn, tightly
Taut, itch, erythema, papule, redness effect.
2. given the test agent: sample 1#, 2# self-control, preparation method are formulated as follows with embodiment 4:
3. experimental method
3.1 14 20-50 years old skins there are shouting pain, burn, the women of tight, itch, erythema, papule, redness it is on probation
Person, average age 40 years old.
3.2 14 patients are randomly divided into two groups, sample sets 1# and sample sets 2#, age, the serious journey of skin problem between two groups
Degree is without marked difference, and face is each primary using lotion sooner or later.It is used in conjunction 8 weeks, two groups of triers is observed weekly, and record disease
Shape alleviates situation and adverse reaction, evaluates after 8 weeks.
3.3 efficacy assessment standards are divided into recovery from illness, effective, effective and invalid, and recovery from illness skin problem subsides completely, subjective symptoms
It disappears.Effective is 60% or more recession, subjective symptoms disappearance or substantially reduced.It is effectively recession 30%~60%, subjective symptoms
Mitigate.It is in vain skin lesion recession less than 30%, subjective symptoms is not improved or unchanged or exacerbation.It fully recovers, is effective and effective
Case joint account total effective rate.
4. result
It after 8 weeks, fully recovers 2 in sample 1# group 7, effective 3, effective 1, invalid 1, total effective rate is
85.71%；It fully recovers 3 in sample 2# group 7, effective 4, effective 1, invalid 1, total effective rate 85.71%.
The experimental results showed that, sample 1# group and sample 2# group can solve skin irritation, burn, is tight, is itch, red above
The problems such as spot, papule, redness, adds the lotion of the Artemisia annua extract containing qinghaosu and qinghaosu without significant difference.
The present composition can play solution skin using qinghaosu or derivatives thereof as one of main active
Skin shouting pain, burn, be tight, itch, erythema, papule, redness the problems such as effect.
The anti-inflammatory effect of 2 present composition of test example
One, test method
1. experiment purpose
Investigate whether the present composition (its active constituent includes qinghaosu and brown algae extract) has anti-inflammatory properties
(effect for inhibiting dimethylbenzene induced mice ear swelling).
2. experimental material
2.1 reagents: chemical industry chemical reagent work, lot number: 201702270B are cloned in dimethylbenzene, Chengdu；Compound dexamethasone acetate
Emulsifiable paste, newly and at holding Group Co., Ltd, lot number: 151201.
2.2 experimental animals: KM mouse, 18-22g, by Sichuan Provincial Academy of Traditional Chinese Medicine animal center provide (SPF grade, qualification
Card number: SCXK (river) 2015-19).
2.3SPF grades of experimental animal environment: room temperature: 18~22 DEG C, relative humidity: 40~70%.Medical experiment animal environment
The facility certificate of competency: SCXK (river) 2015-100.
2.4 given the test agent: 1# sample, 2# sample, 3# sample, 4# sample, self-control, preparation method is with embodiment 4, and formula is such as
Under:
3. experimental method
The experiment of 3.1 xylene-induced ear swelling in mice
(1) dosage grouping and given the test agent give the time
70 mouse, half male and half female are randomly divided into 7 groups, and every group 10, respectively blank control group, positive controls are (multiple
Square dexamethasone acetate emulsifiable paste) and 5 dosage groups.Blank control group two sides before and after mouse right ear applies distilled water (50 μ l/
Only), the two sides before and after mouse right ear applies sample by group setting for administration group and positive controls (0.2g/ is only)；Continuous coating 15
It.
(2) experimental procedure
Blank control group two sides before and after mouse right ear applies distilled water (50 μ l/ are only), and administration group and positive controls press group
Sample She Zhi not be applied in two sides before and after mouse right ear (0.2g/ is only)；Continuous coating 15 days.1h after the last administration, by dimethylbenzene
0.03ml/ is only uniformly applied to mouse right ear tow sides and causes inflammation, and animal is put to death after 30 minutes, two ear ears thickness is surveyed, with two auricles
The difference of thickness is as swelling (mm)；Swelling inhibiting rate (%) is calculated with two auricle thickness differences (mm).
As a result the swelling significance of difference between comparing each group, inspection level α=0.05 are examined with t.
Swelling (mm)=auris dextra thickness (mm)-left ear thickness (mm)
Swelling inhibiting rate (%)=(thick poor (the mm)-sample sets ear of blank control group ear is thick poor (mm))/blank control group ear
Thick poor (mm) * 100%.
Two, experimental result
The lotion paraxylene of the present invention of table 1 causes the influence of mouse ear swelling (ear is thick)
Note: compared with Normal group, * indicates P < 0.05；* indicates P < 0.01.
Find out from the data of mouse auricle thickness, 2#, 3#, 4#, 5# are compared with Normal group for cosmetic sample group, equal energy
Reduce the influence of dimethylbenzene induced mice ear swelling (ear is thick), and swelling is obviously reduced, wherein the anti-inflammatory effect of 2#, 5# sample
With obvious statistical difference (P < 0.01), swelling inhibiting rate has reached 74.01%, 76.51%.
The swelling inhibiting rate of comparative sample group 2#, 3#, 4#, which can be seen that qinghaosu and the compounding of brown algae extract, to be made
With inflammation inhibiting rate can be greatly improved, qinghaosu or brown algae extract is far superior to used alone in antiphlogistic effects.
In drug combination analysis, the Q value method that Nintaus's equalization proposes is a kind of method of common evaluation drug combination effect.
Evaluating two medicines to merge effect is collaboration, superposition or antagonism, and should find out: the practical merging effect/theory of Q=is added merely expected effect
Fruit, Q use following number reflection result property: 0.85~1.15 is added to be simple, and>1.15 be collaboration, and<0.85 is antagonism (ginseng
See: Nintaus equalization estimates the second medical college of new method-" double 30 methods " Shanghai [J] journal of drug combination effect with Q value,
1985,5:353~354).
For the interaction of qinghaosu and brown algae extract in the evaluation present composition, the Q of swelling inhibiting rate is calculated
Value:
P(A+B)=PA+PB-PA·PB=34.35%+16.31%-34.35% × 16.31%=45.06%
Q=PC/P(A+B)=74.01% ÷ 45.06%=1.64 ﹥ 1.15
The result shows that, using swelling inhibiting rate as evaluation index, Q value is big after qinghaosu and brown algae extract are used in compounding
In 1.15, illustrate that two kinds of ingredients have played synergistic effect at anti-inflammatory aspect.On this basis, in the present composition also
It is added to Cordyceps extract, facilitates further increasing for antiphlogistic effects.
3 present composition of test example improves the effect of keratoderma barrier function
One, test formulations
Test lotion is prepared by 4 method of embodiment, is formulated as follows:
Two, experimental method
1,40 20-50 years old sensitive women triers average age 41 years old, are divided into control group and sample sets, early
Late face is each primary using lotion.
2, Finland Delfin VapoMeterVapoMeter moisture evaporates measuring instrument
For testing TEWL value.TEWL value indicates the case where cuticula moisture loss, and TEWL value is higher, and expression is lost through skin
The moisture of mistake is more, and the barrier function of cuticula is poorer；Unit g/m2H, that is, per hour every square metre of area lose how much
Gram water.
It is to measure the index of skin barrier function through epidermis dehydration (transepidermal water loss, TEWL).Such as
The barrier function damage of pericarp skin, loss of moist just will increase, and therefore, become the weight of skin barrier function measurement through epidermis dehydration
Want one of index.Dry skin is the mark of barrier dysfunction, moisture content of skin and skin dryness and mossization skin lesion
It is negatively correlated, TEWL increase, prompt epidermal dysfunction, especially cuticula dysfunction indirectly, often lead to pachylosis,
Furfur, itch, scratching induce scytitis.
Sensibility skin can be come by the test of TWEL value there are the skin that serious skin barrier function is damaged
Judge reparation and improvement of the product to sensibility skin.
3, percutaneous water loss TWEL value is carried out before use, using rear 14d, 28d to test.
4, test position: right face cheek.
5, testing process: laboratory is sat quietly 30 minutes after cleaning, tests TEWL value.
Three, experimental result
The percutaneous water loss TEWL (g/m of 2 skin of table2H) average value
As can be seen from the above table, compared with being not added with the control group of Artemisia annua extract or qinghaosu, sample 1#, sample 2#
Lotion, which can significantly reduce, illustrates that qinghaosu or the Artemisia annua extract containing qinghaosu have obviously through skin water loss
Repair skin barrier function, improve sensitive skin quality effect.Artemisia annua extract and qinghaosu in terms of qinghaosu
Under same additive amount, effect is without significant difference.
In addition, being only added to the sample 3# of Phaeodactylum tricornutum extract, the effect of keratoderma barrier function is improved
4# sample effect that is bad, being used not as good as Artemisia annua extract and Phaeodactylum tricornutum extract combination.Addition Artemisia annua extract,
The 5# sample of Phaeodactylum tricornutum extract and Cordyceps extract, effect are better than 4# sample.
The clinical case of 4 present composition of test example treatment scytitis
1, king XX, female, nonallergic constitution have not found etiology, have caused a disease because occurring due to selecting cosmetics improper
Skin itch, rubescent, in order to mitigate itch, voluntarily external application Hormonal ointment Dexamethasone ointment is smeared, after smearing, in a short time just very
It takes effect fastly, skin itching symptom occurs again after drug withdrawal, and 3 years repeatedly, hospital diagnosis was steroid dependent dermatitis.Proof test example 2
Middle 5# sample, after smearing 1 month, symptom is gradually alleviated, and after 3 months, no longer recurs.
2, Zhao XX, female, skin sensitivity, drying, itch, furfur, the general red tide of face can all occur with any cosmetics
Quick, 5# sample, does not occur allergic phenomena in proof test example 2, and after 1 month, skin quality is obviously improved, skin not re-dry,
Furfur, hence it is evident that improve the symptom of the general red tide of face, itch.
3, Lee XX, female, skin sensitivity select certain online brand cosmetics, are used for a long time, and itch, rubescent, long acne etc. occur
Skin problem, hospital diagnosis are steroid dependent dermatitis.5# sample in proof test example 2, after smearing 1 month, symptom is gradually delayed
Solution, has a rebound during use in the 2nd month, occurs above-mentioned skin problem repeatedly.After 3 months, by 6 months at present, do not have
Have again and above-mentioned skin problem, no relapse occurs.
The above result on trial shows the present invention by qinghaosu or derivatives thereof, Phaeodactylum tricornutum extract and cordyceps sinensis
Extract compounded combination uses, and has the function of releiving, is antipruritic, anti-inflammatory, repair skin damage barrier, reduce redness and itch,
It is especially suitable for sensitivity skin, inflammation skin uses.
Claims (21)
1. the composition of scytitis is prevented and treated, it is characterized in that: the raw material comprising following weight proportion: dihydroartemisinine, sweet wormwood amber
Ester, qinghaosu or 0.10~5.00 part of 0.05~10 part of Artemisia annua extract, brown algae extract containing qinghaosu, worm summer in winter
0.001~1.0 part of grass, wherein the content of phytosterol is 30~40ppm in the brown algae extract.
2. composition as described in claim 1, it is characterized in that: the raw material comprising following weight proportion: dihydroartemisinine, sweet wormwood
Amber ester, qinghaosu or 0.1~2 part of 0.01~1 part of Artemisia annua extract, brown algae extract, cordyceps sinensis containing qinghaosu
0.005~0.2 part；Preferably, the raw material comprising following weight proportion: dihydroartemisinine, Artesunate, qinghaosu contain blueness
0.001~0.03 part of 0.5~2 part of 0.1~0.5 part of Artemisia annua extract, brown algae extract, the cordyceps sinensis of artemisin；Into one
It walks preferably, the raw material comprising following weight proportion: dihydroartemisinine, Artesunate, qinghaosu or the chrysanthemum containing qinghaosu
0.2~0.5 part of parthenium extract, 1~2 part of brown algae extract, 0.02 part of cordyceps sinensis；Most preferably, the weight of the raw material
Proportion are as follows: dihydroartemisinine, Artesunate, qinghaosu or the Artemisia annua extract containing qinghaosu: brown algae extract: winter worm
Summer grass=0.5:2:0.02 or 0.2:1:0.02.
3. composition as claimed in claim 1 or 2, it is characterized in that: the brown algae extract is with chloroform, methanol or it is mixed
What bonding solvent extracted.
4. composition as claimed in claim 3, it is characterized in that: the brown algae extract is to be prepared by the following method to obtain
: brown algae is taken, is extracted with the mixed solvent of chloroform and methanol, crude extract successively uses chloroform, chloroform: acetone volume ratio 3:1's
Mixed solvent carry out silica gel column chromatography classification separation, collect chloroform-acetone eluate to get.
5. composition as claimed in claim 4, it is characterized in that: the in the mixed solvent chloroform of the chloroform and methanol: methanol
Volume ratio is 2:1.
6. composition as claimed in claim 4, it is characterized in that: crude extract is first eluted with chloroform, then with 2 times of volumes of eluent
Chloroform-acetone mixed solvent elution.
7. the composition as described in claim 1~6 any one, it is characterized in that: the brown algae extract is with phaeodactylum tricornutum
Refer to that algae is that raw material extracts to obtain.
8. composition as claimed in claim 7, it is characterized in that: the brown algae extract is with Phaeodactylum tricornutum
Muriella aurantiaca and/or Ruttnera lamellosa microalgae cell is that raw material extracts to obtain.
9. composition as claimed in claim 8, it is characterized in that: Muriella aurantiaca in the brown algae extract
The mass ratio of microalgae cell extract and Ruttnera lamellosa microalgae cell extract is 1:1.
10. composition as claimed in claim 1 or 2, it is characterized in that: the cordyceps sinensis is section ergot fungus cordyceps sinensis
Bacterium Cordyceps sinensis (BerK.) Sacc. parasitism stroma on the larvae of batrachidae insects and larva corpse it is dry
Dry complex.
11. composition as claimed in claim 10, it is characterized in that: the cordyceps sinensis is added in the form of primary powder or extract
Add.
12. composition as claimed in claim 11, it is characterized in that: the extract is water extract.
13. composition as claimed in claim 12, it is characterized in that: in the extract, polysaccharide >=1mg/L, mannitol >=
1mg/L, adenosine >=1mg/L；Preferably, in the extract, polysaccharide >=500mg/L, mannitol >=700mg/L, adenosine >=
5mg/L。
14. the composition as described in claim 1~13 any one, it is characterized in that: being with the raw material of each weight proportion for work
Property ingredient, is added acceptable auxiliary material or complementary ingredient, drug, the cosmetics being prepared.
15. composition as claimed in claim 14, it is characterized in that: the cosmetics be Essence, lotion, freeze-dried powder, creme,
Gel, facial mask, foundation cream, muffin, facial cleanser or aqua.
16. composition as claimed in claim 14, it is characterized in that: the drug is external preparation.
17. the preparation method of composition described in claim 1~16 any one, it is characterized in that: including the following steps: to take each heavy
Measure the raw material of proportion, drug is added, in cosmetics acceptable auxiliary material or complementary ingredient to get.
18. use of the composition described in claim 1~16 any one in the drug, cosmetics of preparation prevention and treatment scytitis
On the way.
19. purposes as claimed in claim 18, it is characterized in that: the prevention and treatment scytitis is to alleviate skin irritation, burn, tightly
Taut, itch, erythema, papule or redness.
20. drug, makeup that composition described in claim 1~16 any one improves keratoderma barrier function in preparation
Purposes in product.
21. purposes as claimed in claim 20, it is characterized in that: the raising keratoderma barrier function is to reduce moisture warp
Keratoderma is lost.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810122515.7A CN110115697A (en) | 2018-02-07 | 2018-02-07 | The composition of prevention and treatment scytitis containing cordyceps sinensis |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810122515.7A CN110115697A (en) | 2018-02-07 | 2018-02-07 | The composition of prevention and treatment scytitis containing cordyceps sinensis |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1499957A (en) * | 2001-04-03 | 2004-05-26 | Lvmh����Ⱦ������漯�� | Use of phaeodactylum algae extract to promote proteasome activity of skin cells |
| CN106420381A (en) * | 2016-08-08 | 2017-02-22 | 辛成业 | Novel applications of artemisinin ingredients or composition of artemisinin ingredients in skin care |
-
2018
- 2018-02-07 CN CN201810122515.7A patent/CN110115697A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1499957A (en) * | 2001-04-03 | 2004-05-26 | Lvmh����Ⱦ������漯�� | Use of phaeodactylum algae extract to promote proteasome activity of skin cells |
| CN106420381A (en) * | 2016-08-08 | 2017-02-22 | 辛成业 | Novel applications of artemisinin ingredients or composition of artemisinin ingredients in skin care |
Non-Patent Citations (1)
| Title |
|---|
| 董素敏: ""虫草在化妆品中的应用"", 《中国化妆品》 * |
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