CN110108869A - For cyclic group because chronic obstructive pneumonia early stage rapid screening papery micro-fluidic chip detection card and its test strips - Google Patents
For cyclic group because chronic obstructive pneumonia early stage rapid screening papery micro-fluidic chip detection card and its test strips Download PDFInfo
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- CN110108869A CN110108869A CN201910324382.6A CN201910324382A CN110108869A CN 110108869 A CN110108869 A CN 110108869A CN 201910324382 A CN201910324382 A CN 201910324382A CN 110108869 A CN110108869 A CN 110108869A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54346—Nanoparticles
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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Abstract
The present invention relates to it is a kind of for cyclic group because chronic obstructive pneumonia early stage rapid screening papery micro-fluidic chip detection card and its test strips, including upper Ka Gai and lower Ka Gai, lower card is covered equipped with upper Ka Gai, the inner surface of the lower Ka Gai is equipped with test strips groove, is equipped with test strips in test strips groove；Sample pad, gold-labelled pad, nitrocellulose filter and the sample suction pad that the test strips include PVC bottom plate and are successively overlapped on PVC bottom plate；Test strips in the invention have the characteristics of detection high specificity, high sensitivity；The invention uses widely applied, efficient base complementrity and compound association reaction is detected, the level of albumen in blood sample can accurately be detected, and be combined and capture using the gene probe and compound of high-quality, greatly improve nucleic acid detection test strip sensitivity.
Description
Technical field
The present invention relates to it is a kind of for cyclic group because chronic obstructive pneumonia early stage rapid screening papery micro-fluidic chip
Detection card and its test strips, belong to field of biomedicine.
Background technique
Chronic Obstructive Pulmonary Disease (COPD) is the disease with flow limitation feature, and flow limitation is not fully reversible,
Can be with airway hyperreactivity, and be in carry out sexual development.Reacting with abnormal inflammatory of the lung to pernicious gas or deleterious particle has
It closes.COPD is a kind of disease for seriously endangering common people's health, occupies the 4th of the global cause of death at present, arrives the year two thousand twenty
The 3rd big cause of the death will be become.COPD also creates the financial burden of tight gill fungus, and the World Bank/World Health Organization (WHO) announces, until
The year two thousand twenty, COPD will become the 5th of world's disease financial burden.(WHO) assessment c0PD is the of China's disease financial burden
One.
Due to human body respiratory function have huge compensatory capacity, early stage COPD due to body is compensatory and disease still
It is clinical uncomfortable how not significant in early stage, or even without clinical symptoms.And the progress of COPD is a slow process, is risen
First human body can gradually adapt to this, be not easy to cause the attention of patient.Once there is symptom, Pulmonary Function detraction has reached 30%-
50%, it is even more.Hereafter, as long as lung function has slight decline, patient respiratory degree of difficulty will exponentially rising.Therefore,
The asymptomatic COPD patient of early detection has important meaning.
The goldstandard of current diagnosis --- pulmonary function test (Forced expiratory volume in one second (FEV1)/forced vital capacity
(FVC) < 70%) to accuracy of instrument, place it is more demanding, take a long time, be not suitable for the environment such as community or factory under big rule
Mould rapid screening.
Summary of the invention
The present invention be directed to the deficiencies in the prior art, provide it is a kind of for cyclic group because chronic obstructive pneumonia
Early stage rapid screening papery micro-fluidic chip detection card and its test strips, to solve problems of the prior art.
To achieve the above object, The technical solution adopted by the invention is as follows:
It is a kind of for cyclic group because chronic obstructive pneumonia early stage (hsa_circ_0005045 cyclic group because) rapid screening paper
Matter micro-fluidic chip detection card and its test strips, including upper Ka Gai and lower Ka Gai, lower card are covered equipped with upper Ka Gai, the lower card
The inner surface of lid is equipped with test strips groove, is equipped with test strips in test strips groove；The test strips include PVC bottom plate and successively
Sample pad, gold-labelled pad, nitrocellulose filter and the sample suction pad being overlapped on PVC bottom plate.
As an improvement of the present invention, the sample pad includes the adsorption layer being successively configured from top to bottom, filtering
Layer and sample layer.
As an improvement of the present invention, the adsorption layer is cellular solid layer, and the filter layer is qualitative filter paper.
As an improvement of the present invention, the sample suction pad includes blotting paper, and blotting paper lower surface is equipped with porosu solid material
The bed of material.
As an improvement of the present invention, the chromatographic film is nitrocellulose filter, is arranged on the nitrocellulose filter
There are detection line T line and nature controlling line C line.
As an improvement of the present invention, the nature controlling line C line is coated with biotin Biotin, the detection line T line packet
There is anti-FITC antibody.
As an improvement of the present invention, the gold-labelled pad includes glass fibre membrane, sprayed on glass fibre membrane golden liquid and
Probe dilution liquid, the gold liquid are the colloid gold particle of labelled streptavidin, and the probe dilution liquid dissolves for no enzyme water to be visited
The liquid of needle 1 and probe 2, the nucleotides sequence list of the probe 1 is as shown in SEQ ID NO:1；The nucleotides sequence of the probe 2
List is as shown in SEQ ID NO:2；
SEQ ID NO:1:5'-biotin-GAAATCAGATGAAGGCCACCC-FITC-3'
SEQ ID NO:2:5'-biotin-TGTCAATGAAAGCAGCAGGAAT-FITC-3'.
As an improvement of the present invention, the inner surface surrounding of the upper Ka Gai is equipped with hold-down bars, the lower Ka Gainei
Surface is equipped with cylindrical groove corresponding with hold-down bars, for fastening with upper card lid fixing compact.
As an improvement of the present invention, the outer surface of the upper Ka Gai is equipped with anti-skidding hand-held groove one, the antiskid
Hold groove one be arranged on the right side of Shang Kagai, the lower card exterior surface be equipped with anti-skidding hand-held groove two, it is described it is anti-skidding hold it is recessed
Slot one is adapted with anti-skidding hand-held groove two.When the Shang Kagai and lower card cover buckle are closed, the upper card covers anti-skidding hand-held groove one
Anti-skidding hand-held groove two is covered with lower card and also realizes fastening, forms hand holding handle, and the outer surface of the hand holding handle is anti-skid chequer.Hand holding handle
The hand-held card of operator can be made more convenient, reduction is touched with communicable sample, to reduce infection risk；
As an improvement of the present invention, the upper card covers is successively arranged well and observation window from left to right, described to add
Sample hole is adapted with test strips groove；The observation window is adapted with test strips groove.Detection card is equipped with observation window, when
When Shang Kagai and lower card are stamped fastened, tester is developed the color by the upper observation window covered i.e. observable test strips of blocking；Simultaneously
Well directly is offered in Shang Kagai compared to traditional detection card, when Shang Kagai and lower card lid mouth fasten, upper Ka Gai is by sample
Product isolation, makes testing staff observe testing result safe and conveniently.
Due to using the above technology, the present invention compared with the prior art, is had the advantage that as follows:
(1) test strips in the invention have the characteristics of detection high specificity, high sensitivity.The invention uses wide
General application, efficient base complementrity and compound association reaction are detected, and the level of albumen in blood sample can be accurately detected, and
It is combined and captures using the gene probe and compound of high-quality, greatly improve nucleic acid detection test strip sensitivity.
(2) test strips in the invention are easy to operate, and result interpretation is simple, are not necessarily to specific apparatus, technical requirements
It is low, the one of the personnel that are screened need to only be bled and be dripped in test strip, and only need a few minutes reaction can directly visually
Observation is low to experimenter's technical requirements without specific apparatus as a result, result interpretation is intuitive, can be widely applied to base
And remote countryside medical institutions.
(3) the test strips testing result in the invention is quick, and time-consuming short and cheap, testing cost is low.Pass through
The test strips are detected, and only need 5min or so that can carry out result interpretation, though this method is detection nucleic acid, expense is more than existing
The expense of some fluorescent quantitations and genetic chip is low.
It (4) include adsorption layer in the sample pad of the invention test strips, which selects adsorbable and inactivation disease
The cellular solid production of poison has adsorption layer and is directed to while not influencing hsa_circ_0005045 detection effect
Adsorb and inactivate the specificity of other potential pathogens；And be also added in the sample suction pad of test strips one layer it is treated porous solid
Body material further can be adsorbed and be inactivated to blood sample after detection, and further decreasing can on detection card after the completion of detecting
Pathogen existing for energy gives testing staff's bring risk.
Detailed description of the invention
Fig. 1 is the main view that detection blocks upper Ka Gai；
Fig. 2 is the bottom view that detection blocks upper Ka Gai；
Fig. 3 is the rearview that detection blocks upper Ka Gai；
Fig. 4 is the main view that detection blocks lower Ka Gai；
Fig. 5 is the top view that detection blocks lower Ka Gai；
Fig. 6 is the rearview that detection blocks lower Ka Gai；
Fig. 7 is the main view of test strips；
Fig. 8 is the bottom view of test strips；
Fig. 9 be detection card for scrutineer's early stage rapid screening chronic obstructive pneumonia hsa_circ_0005045 cyclic group because
The test result of detection；
1, Shang Kagai, 2, Xia Kagai, 3, test strips groove, 4, test strips, 5, PVC bottom plate, 6, sample pad, 7, gold-labelled pad, 8, nitre
Acid cellulose film, 9, sample suction pad, 10, hold-down bars, 11, cylindrical groove, 12, anti-skidding hand-held groove one, 13, anti-skidding hand-held groove two,
14, well, 15, observation window, 16, detection line, 17, nature controlling line.
Specific embodiment
With reference to the accompanying drawings and detailed description, the present invention is furture elucidated.
Embodiment 1
It is a kind of for cyclic group because chronic obstructive pneumonia early stage (hsa_circ_0005045 cyclic group because) rapid screening paper
Matter micro-fluidic chip detection card and its test strips, including upper card lid 1 and lower card lid 2, lower card lid 2 is equipped with upper card lid 1, described
The inner surface of lower card lid 2 is equipped with test strips groove 3, is equipped with test strips 4 in test strips groove 3；The test strips 4 include the bottom PVC
Plate 5 and the sample pad 6 being successively overlapped on PVC bottom plate 5, gold-labelled pad 7, nitrocellulose filter 8 and sample suction pad 9.
The inner surface surrounding of the upper card lid 1 is equipped with hold-down bars 10, and lower 2 inner surface of card lid is equipped with and hold-down bars 10
Corresponding cylindrical groove 11.
The outer surface of the upper card lid 1 is equipped with anti-skidding hand-held groove 1, and the anti-skidding hand-held groove 1 is arranged upper
The right side of card lid 1, lower 2 outer surface of card lid are equipped with anti-skidding hand-held groove 2 13, the anti-skidding hand-held groove 1 with it is anti-skidding
Hand-held groove 2 13 is adapted.
Well 14 and observation window 15, the well 14 and test paper are successively arranged on the upper card lid 1 from left to right
Groove 3 is adapted；The observation window 15 is adapted with test strips groove 3.
Embodiment 2
The present embodiment is to provide the preparation method of test strips 4 described in embodiment 1.
1, the preparation of hsa_circ_0005045 probe antibody and SA label colloid gold particle
Colloidal gold is prepared using trisodium citrate reduction method: 200ml deionized water being added to and is crossed through bubble acid and silicidation
In round-bottomed flask, and it is heated and is boiled with electric jacket, 200r/min stirring；1% chlorauric acid solution of 2ml is rapidly added to boiling
It rises；6ml citric acid three sodium solution is added, solution gradually becomes claret, until 10min is reheated after color is constant, it is rear to stop adding
Heat stirs 15min；Restore to room temperature, restores volume to 200ml, 4 DEG C of refrigerators save.
It is public purchased from thermo fisher by synthetic probe 1 and probe 2(SEQ ID NO:1 and SEQ ID NO:2
Department, final concentration is respectively 100 μM) it is added to TCEP-HCl(final concentration 100mM) in, restore sulfhydrylation probe；Will treated visit
Needle is added in the colloidal gold liquid of 30nm diameter particle, and ambient temperature overnight is incubated for；2%SDS liquid, which is added, in next day keeps its final concentration of
0.01%, it is incubated at room temperature 30min；The NaCl of 2M is added dropwise into solution, until final concentration of 0.15M.15000rpm centrifugation
15min, precipitating are washed four times with cleaning solution (0.15MNaCl, 0.01%SDS), by the gold mark nucleic acid probe colloidal gold after centrifugal purification
Precipitating is resuspended in re-suspension liquid (0.15MNaCl, 5%BSA, 0.25%Tween, 10% sucrose), and the general nucleic acid that must have both marked is visited
Needle marks colloid gold particle.Immune colloid gold will be prepared and be added drop-wise to gold-labelled pad 7(glass fibre membrane) on, it is naturally dry at room temperature
It is dry, sealing.
2, chronic obstructive pneumonia hsa_circ_0005045 cyclic group is because of papery micro-fluidic chip test strips 4 and detection card
Preparation
The main raw material(s) needed when preparing nucleic acid detection test strip 4: glass fibre membrane, nitrocellulose filter 8, sample pad,
PVC bottom plate 5 etc..
(1) glass fibre membrane that immune colloid gold has been added dropwise the preparation of gold-labelled pad 7: is cut into the small mould of 0.5 × 1cm square
Block, Seal and preservation are spare.
(2) spray film: anti-FITC antibody is used for detection line 16(T line) display, biotin Biotin is for nature controlling line 17(C line)
Display.Nitrocellulose filter 8 is placed on 1000 specking instrument platform of Biodot ZX, two antibody are put in sample cell respectively,
Machine is opened, allowing anti-FITC antibody and biotin Biotin to distinguish fixed fire in forming two lines, dosage on film is 3 μ l/cm, sprays film
After, film item is put in 37 DEG C of clean insulating boxs drying 5-6 hours, is stored in spare in dry environment；
(3) test strips 4 and detection card assembling
Nitrocellulose filter 8 is affixed among PVC bottom plate 5, then by sample suction pad 9 and gold-labelled pad 7 side to be overlapped slightly respectively
Formula is affixed on 8 two sides of nitrocellulose filter, then sample pad 6 is pushed down gold-labelled pad 7 slightly and is affixed on bottom plate；Use Biodot
CM4000 cutting machine is cut into the test strips 4 of 4mm, and test strips 4 are put into the corresponding groove of lower card lid 2, each with upper card lid 1
Hold-down bars 10, buckle fasten one by one, it is ensured that well 14 and observation window position are errorless, 4 DEG C of preservations.
3, chronic obstructive pneumonia hsa_circ_0005045 cyclic group detects COPD because papery micro-fluidic chip detects card
The content of hsa_circ_0005045 in patients serum
It takes 100ul COPD patient and physical examination of healthy population fresh serum sample to be slowly added dropwise to sample pad 6 respectively respectively, will try
Paper lays flat 5-7min, if measuring samples are the positive, test strips 4 will appear two red stripes；If measuring samples are feminine gender,
Only occur a red stripes at C line；If no band generates or only occur at T line vitta band altogether, illustrate this test strips 4
It is no longer valid.Color change image is acquired with taking pictures, detects the content of hsa_circ_0005045 in blood.
Above-described embodiment is only the preferred technical solution of the present invention, and is not construed as limitation of the invention, the present invention
Protection scope should with claim record technical solution, including claim record technical solution in technical characteristic etc.
It is protection scope with alternative, i.e., equivalent replacement within this range is improved, also within protection scope of the present invention.
Sequence table
<110>Southeast China University
<120>for cyclic group because chronic obstructive pneumonia early stage rapid screening papery micro-fluidic chip detection card and its examination
Paper slip
<160> 2
<170> SIPOSequenceListing 1.0
<210> 1
<211> 21
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 1
gaaatcagat gaaggccacc c 21
<210> 2
<211> 22
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 2
tgtcaatgaa agcagcagga at 22
Claims (10)
1. it is a kind of for cyclic group because chronic obstructive pneumonia early stage rapid screening papery micro-fluidic chip detection card and its examination
Paper slip, it is characterised in that: including upper Ka Gai and lower Ka Gai, lower card is covered equipped with upper Ka Gai, and the inner surface of the lower Ka Gai is set
There is test strips groove, is equipped with test strips in test strips groove；The test strips include PVC bottom plate and are successively overlapped on the bottom PVC
Sample pad, gold-labelled pad, nitrocellulose filter and sample suction pad on plate.
2. it is according to claim 1 it is a kind of for cyclic group because chronic obstructive pneumonia early stage rapid screening papery miniflow
Control chip detection card and its test strips, it is characterised in that: the sample pad include the adsorption layer being successively configured from top to bottom,
Filter layer and sample layer.
3. it is according to claim 2 it is a kind of for cyclic group because chronic obstructive pneumonia early stage rapid screening papery miniflow
Control chip detection card and its test strips, it is characterised in that: the adsorption layer is cellular solid layer, and the filter layer is qualitative
Filter paper.
4. it is according to claim 1 it is a kind of for cyclic group because chronic obstructive pneumonia early stage rapid screening papery miniflow
Control chip detection card and its test strips, it is characterised in that: the sample suction pad includes blotting paper, and blotting paper lower surface is equipped with porous solid
Body material layer.
5. it is according to claim 1 it is a kind of for cyclic group because chronic obstructive pneumonia early stage rapid screening papery miniflow
Control chip detection card and its test strips, it is characterised in that: the chromatographic film is nitrocellulose filter, on the nitrocellulose filter
It is provided with detection line T line and nature controlling line C line.
6. it is according to claim 5 it is a kind of for cyclic group because chronic obstructive pneumonia early stage rapid screening papery miniflow
Control chip detection card and its test strips, it is characterised in that: the nature controlling line C line is coated with biotin Biotin, the detection line T
Line is coated with anti-FITC antibody.
7. it is according to claim 1 it is a kind of for cyclic group because chronic obstructive pneumonia early stage rapid screening papery miniflow
Control chip detection card and its test strips, it is characterised in that: the gold-labelled pad includes glass fibre membrane, sprays gold on glass fibre membrane
Liquid and probe dilution liquid, the gold liquid are the colloid gold particle of labelled streptavidin, and the probe dilution liquid is water-soluble for no enzyme
The liquid of probe 1 and probe 2 is solved, the nucleotides sequence list of the probe 1 is as shown in SEQ ID NO:1；The nucleosides of the probe 2
Acid sequence table is as shown in SEQ ID NO:2；
SEQ ID NO:1:5'-biotin-GAAATCAGATGAAGGCCACCC-FITC-3'
SEQ ID NO:2:5'-biotin-TGTCAATGAAAGCAGCAGGAAT-FITC-3'.
8. it is according to claim 1 it is a kind of for cyclic group because chronic obstructive pneumonia early stage rapid screening papery miniflow
Control chip detection card and its test strips, it is characterised in that: the inner surface surrounding of the upper Ka Gai is equipped with hold-down bars, the lower card
Interior surface is equipped with cylindrical groove corresponding with hold-down bars.
9. it is according to claim 1 it is a kind of for cyclic group because chronic obstructive pneumonia early stage rapid screening papery miniflow
Control chip detection card and its test strips, it is characterised in that: the outer surface of the upper Ka Gai is equipped with anti-skidding hand-held groove one, described anti-
Skating is held groove one and is arranged on the right side of Shang Kagai, and the lower card exterior surface is equipped with anti-skidding hand-held groove two, the antiskid
Groove one is held to be adapted with anti-skidding hand-held groove two.
10. it is according to claim 1 it is a kind of for cyclic group because chronic obstructive pneumonia early stage rapid screening papery it is micro-
Fluidic chip detection card and its test strips, it is characterised in that: the upper card covers is successively arranged well and observation from left to right
Form, the well are adapted with test strips groove；The observation window is adapted with test strips groove.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910324382.6A CN110108869A (en) | 2019-04-22 | 2019-04-22 | For cyclic group because chronic obstructive pneumonia early stage rapid screening papery micro-fluidic chip detection card and its test strips |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910324382.6A CN110108869A (en) | 2019-04-22 | 2019-04-22 | For cyclic group because chronic obstructive pneumonia early stage rapid screening papery micro-fluidic chip detection card and its test strips |
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| Publication Number | Publication Date |
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| CN110108869A true CN110108869A (en) | 2019-08-09 |
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ID=67486258
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|---|---|---|---|
| CN201910324382.6A Pending CN110108869A (en) | 2019-04-22 | 2019-04-22 | For cyclic group because chronic obstructive pneumonia early stage rapid screening papery micro-fluidic chip detection card and its test strips |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1811447A (en) * | 2006-02-08 | 2006-08-02 | 杭州优思达生物技术有限公司 | Nucleic acid membrane chromatographic fast detecting method and its test paper bar and use thereof |
| CN107937514A (en) * | 2017-11-30 | 2018-04-20 | 南昌大学第附属医院 | Applications of the circZFY in pulmonary tuberculosis biomarker |
| CN109295253A (en) * | 2018-11-16 | 2019-02-01 | 山东省花生研究所 | High oleic acid peanut is quickly chosen seeds kit |
| CN109406781A (en) * | 2018-12-27 | 2019-03-01 | 正元盛邦(天津)生物科技有限公司 | A kind of HIV(1+2 reducing infection risk) antibody test card and its adaptation test strips |
-
2019
- 2019-04-22 CN CN201910324382.6A patent/CN110108869A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1811447A (en) * | 2006-02-08 | 2006-08-02 | 杭州优思达生物技术有限公司 | Nucleic acid membrane chromatographic fast detecting method and its test paper bar and use thereof |
| CN107937514A (en) * | 2017-11-30 | 2018-04-20 | 南昌大学第附属医院 | Applications of the circZFY in pulmonary tuberculosis biomarker |
| CN109295253A (en) * | 2018-11-16 | 2019-02-01 | 山东省花生研究所 | High oleic acid peanut is quickly chosen seeds kit |
| CN109406781A (en) * | 2018-12-27 | 2019-03-01 | 正元盛邦(天津)生物科技有限公司 | A kind of HIV(1+2 reducing infection risk) antibody test card and its adaptation test strips |
Non-Patent Citations (2)
| Title |
|---|
| SALZMAN，ET AL: "Salzman et al. 2013 circular RNA candidates (hsa_circ_0005045)", 《HTTP://WWW.CIRCBASE.ORG/CGI-BIN/SIMPLESEARCH.CGI》 * |
| ZIKUN HUANG,ET AL: "Circulating circular RNAs hsa_circ_0001204 and hsa_circ_0001747 act as diagnostic biomarkers for active tuberculosis detection", 《INT J CLIN EXP PATHOL》 * |
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Application publication date: 20190809 |