CN110106260A - The combination of the SNP marker of Fengjing pig and raw meat product and identification method - Google Patents
The combination of the SNP marker of Fengjing pig and raw meat product and identification method Download PDFInfo
- Publication number
- CN110106260A CN110106260A CN201910462663.8A CN201910462663A CN110106260A CN 110106260 A CN110106260 A CN 110106260A CN 201910462663 A CN201910462663 A CN 201910462663A CN 110106260 A CN110106260 A CN 110106260A
- Authority
- CN
- China
- Prior art keywords
- pig
- site
- genotype
- fengjing
- identification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of combination of the SNP marker of Fengjing pig and raw meat product and identification methods, by the genomic DNA for extracting raw pork or meat products, it is sequenced through the laggard row agarose gel electrophoresis of PCR amplification and Sanger, according to the SNP genotype identification Fengjing pig and its meat products in sequencing result feature site；The Sanger sequencing, identifies site are as follows: pig17-12719441, pig1-156032177, pig14-83975934 and pig10-62388272 specific mutagenesis occur at site；The present invention solves the problems, such as that there has been no Fengjing pig and its relevant identification methods of meat products in the prior art.
Description
Technical field
The present invention relates to a kind of food safety monitoring fields, and in particular to the SNP of a kind of Fengjing pig and raw meat product
Label combination and identification method.
Background technique
Taihu Lake basin local varieties pig mainly has: Erhualian, plum mountain pig, Fengjing pig, husky rhizome of Chinese monkshood pig, rice pig, Jiaxing Black
Pig, wherein plum mountain pig because body size is divided into middle plum mountain pig and little MeiShan pig.Taihu Lake basin local varieties pig is and each with root common source
There is the case where obscuring in production in tool feature, but because its appearance traits is close, especially piglet, and by traditional figure outside
It is larger that each kind of Taihu Lake basin local varieties pig is distinguished difficulty by looks cultivar identification method, is unfavorable for effectively carrying out guarantor
Kind and development and utilization work.On the other hand, Taihu Lake basin local varieties Meat is delicious and extensively get consumer reception, economic valence
Value is higher than the filial generations porks such as the common Duroc in market, long white, Large White, thus occurs above-mentioned filial generation in the market
The case where Taihu Lake basin local varieties pork sale of pork personation, and above-mentioned hybridization pork is adulterated in Taihu Lake basin place
The sales behavior of kind pork product (such as ham, meat stuffing etc.), but conventional method can not cut meat to slaughter and treated
Meat products carries out accurate ownership judgement.Single nucleotide polymorphism (Single nucleotide polymorphism, SNP) is main
Refer to DNA sequence polymorphism caused by a single nucleotide variation at the genomic level, there is site to enrich, be distributed extensively
It is general, genetic stability is high, it is representative, detection it is convenient and quick the features such as.And wherein, in certain kind (group) range
Interior, the SNP only occurred in a group is referred to as the special SNP of kind.The special SNP site screening of kind and Sites Combination method
Research is the key that carry out kind and product identification.
Third generation molecular marker SNP has variation abundant, low, steady to DNA sample requirement relative to preceding two generations molecular labeling
The advantages that qualitative high, measurement is accurate, detection method is easy and flux is high.Currently, third generation molecular marker SNP is widely used to
The fields such as paternity test, plant and animal species (strain) identification, genetic breeding.
Summary of the invention
In view of the above-mentioned deficiencies in the prior art, it is an object of the present invention to propose a kind of SNP for identifying Fengjing pig and raw meat product
Label combination and identification method, are identified using third generation molecular labeling and Sanger sequencing technologies identify Fengjing pig and raw meat system
Product solve the problems, such as that there has been no Fengjing pig and its relevant identification methods of meat products in the prior art, and it is quasi- to provide a kind of result
Really, the method for easy to operate, cheap identification Fengjing pig and its meat products and related primer special.
The purpose of the present invention is what is be achieved through the following technical solutions:
A kind of combination of the SNP marker of Fengjing pig and raw meat product, comprising: pig17-12719441, pig1-156032177,
Pig14-83975934 and pig10-62388272；The pig17-12719441 indicates No. 17 chromosomes of pig the 12719441st
Site, pig1-156032177 indicate the 156032177th site of No. 1 chromosome of pig, and pig14-83975934 indicates pig No. 14
The 83975934th site of chromosome, pig10-62388272 indicate the 62388272nd site of No. 10 chromosomes of pig.
Further, Fengjing pig and its raw meat product identification method based on SNP marker combination, are extracted to be identified first
Then the genomic DNA of raw pork or meat products is sequenced through the laggard row agarose gel electrophoresis of PCR amplification and Sanger, obtains
SNP marker combines the information of each detection site, specific mutagenesis, Ji Kejian occurs when SNP marker combines at any three sites
It is set to Fengjing pig and its meat products.
Further, the PCR amplification, the sequence such as SEQ ID of the upstream and downstream primer at pig17-12719441
The sequence of upstream and downstream primer at pig1-156032177 shown in NO.1~2 is as shown in NO.3~4 SEQ ID, pig14-
Upstream and downstream primer of the sequence of upstream and downstream primer at 83975934 as shown in NO.5~6 SEQ ID, at pig10-62388272
Sequence as shown in NO.7~8 SEQ ID.
Further, sequencing product identification site comparative information is as shown in the table:
| SNP | REF | ALT |
| pig17-12719441 | A | G |
| pig1-156032177 | C | T |
| pig14-83975934 | G | A |
| pig10-62388272 | G | T |
REF, which is represented, in table refers to genotype, and ALT represents mutated-genotype.
Further, the identification site information are as follows: the mutated-genotype of each detection site with refer to genotype, when
Pig detection site to be measured thinks that the site does not have Identification Significance when occurring with reference to genotype, when pig detection site to be measured is mutated
When genotype, it is believed that the site has Identification Significance.The beneficial effects of the present invention are: compared with prior art, the present invention is with maple
Pig variety peculiar SNP site in river rising in Ningxia and flowing into central Shaanxi is appraisal basis, the method for identifying Fengjing pig is studied from molecular level, and be with Sanger sequencing
Main molecules identification method can distinguish Fengjing pig and other pig variety (strain) mutual authentications, and can by Fengjing pig and often
See that Modern China identification is distinguished, such as: little MeiShan pig, rice pig, middle plum mountain pig, Erhualian, Jiaxing Black Pig, husky rhizome of Chinese monkshood pig, length
White pig, Large White, Duroc, Pietrain, Berkshire etc..
Specific embodiment
The specific embodiment of the invention is described in further detail below.
The present invention relates to the SNP markers of a kind of Fengjing pig and raw meat product, comprising: pig17-12719441, pig1-
156032177, pig14-83975934 and pig10-62388272；The pig17-12719441 indicates No. 17 chromosomes of pig the
12719441 sites, pig1-156032177 indicate the 156032177th site of No. 1 chromosome of pig, pig14-83975934
Indicate the 83975934th site of No. 14 chromosomes of pig, pig10-62388272 indicates the 62388272nd position of No. 10 chromosomes of pig
Point.
The present invention provides a kind of Fengjing pigs and raw meat product identification method based on the combination of above-mentioned SNP marker, mention first
The genomic DNA of raw pork or meat products to be identified is taken, then through the laggard row agarose gel electrophoresis of PCR amplification and Sanger
Sequencing, obtains the information that SNP marker combines each detection site, special dash forward occurs when SNP marker combines at any three sites
Become, Fengjing pig and its meat products can be accredited as.
The PCR amplification, the primer being directed to are as shown in table 1:
Table 1 expands site primer and resulting information
F represents upstream primer in table, and R represents downstream primer, and length represents standardized products length, F'-position generation
Table SNP site is in the position of amplified production.
The PCR amplification, reaction system are template DNA 1ng/uL, primer 1uL, H2O 3.8uL、2×Taq PCR
Masrer Mix 50uL；And/or the response procedures of PCR reaction are 95 DEG C of initial denaturation 2min, 95 DEG C of initial denaturation 30s, 60
DEG C annealing temperature 30s, 72 DEG C of extension 1min, cycle-index 30 times, 72 DEG C re-extend 10min.
The mass concentration of the Ago-Gel is 2%.
The gel electrophoresis, band length requirement is as shown in table 1.
It is as shown in table 2 that product identification site comparative information is sequenced:
Table 2 is sequenced product and identifies site comparative information
| SNP | REF | ALT |
| pig17-12719441 | A | G |
| pig1-156032177 | C | T |
| pig14-83975934 | G | A |
| pig10-62388272 | G | T |
REF, which is represented, in table refers to genotype, and ALT represents mutated-genotype.
As shown in table 2, the mutated-genotype of each detection site with refer to genotype, when pig detection site to be measured is joined
Think that the site does not have Identification Significance when examining genotype, when mutated-genotype occurs in pig detection site to be measured, it is believed that the site
With Identification Significance, such as a pig A to be measured is in the site pig17-12719441, if sequencing data is A, then it is assumed that pig to be measured
A does not have Identification Significance in the site pig17-12719441；If sequencing data is G, then it is assumed that pig A to be measured is in pig17-
12719441 sites have Identification Significance.
Since there are false positive misjudgement as appraisal basis for single locus, and it is higher to misjudge probability, so present invention benefit
Use Sites Combination as identification of M arker.
Each cultivar identification Sites Combination identification of M arker information is as shown in table 3.
3 identification marking combined information of table
As shown in table 3, Marker1-4 is the combination of Fengjing pig identification marking, such as when pig A to be measured has 4 Marker groups
When any one site mutation in conjunction combines, then it is assumed that pig A to be measured is Fengjing pig.
The Fengjing pig meat products refer to Fengjing pig cut meat and the pickled product that is prepared using Fengjing pig as Raw material processing and
Cooked product.
5 parts of pig kind ear tissue sample to be measured are randomly selected, tissue DNA is extracted using SDS method.
PCR reaction amplification is carried out using primer pair DNA sample.
The reaction system of PCR reaction is 10uL system: template DNA 1ng/uL, primer 1uL, H2O 3.8uL、2×Taq
PCR Masrer Mix 50uL；And/or the response procedures of PCR reaction are 95 DEG C of initial denaturation 2min, 95 DEG C of initial denaturation 30s, 60
DEG C annealing temperature 30s, 72 DEG C of extension 1min, cycle-index 30 times, 72 DEG C re-extend 10min.
With 2% Ago-Gel, 1 × TAE buffer is that dielectrophoresis detects amplification, and Gel electrophoresis conditions are electricity
Flow 10A, 100 volts of voltage, the time 40 minutes.The amplified production of different primers is compared with the standardized products length in table 1, produces
Object length is in error range and consistent with standardized products length, then it is assumed that amplification is qualified.
Qualified sample pcr amplification product is subjected to Sanger sequencing, obtains the information of each detection site, sequencing is tied
Fruit is analyzed:
4 sample sequencing result SNP polymorphism analysis table of table
REF, which is represented, in table refers to genotype, and ALT represents mutated-genotype, and √ representative detects mutated-genotype.
As shown in table 4, it can be obtained by sequencing data, if one pig to be measured is simultaneously only with single site as appraisal basis
Belong to multiple kinds.
Identified in the way of Sites Combination Marker: No. 1 pig is in pig17-12719441 site primer to prominent
Become, does not meet Marker information, identify that No. 1 pig is not Fengjing pig；No. 2 pigs pig17-12719441, pig1-156032177,
Pig14-83975934 site primer meets Marker1 information, identifies that No. 2 pigs are Fengjing pigs to mutation；No. 3 pigs are in pig1-
156032177, pig14-83975934 site primer does not meet Marker information, identifies that No. 3 pigs are not Fengjing pigs to mutation；4
Number pig, to mutation, does not meet Marker information, identifies No. 4 pigs in pig17-12719441, pig10-62388272 site primer
It is not Fengjing pig；No. 5 pigs pig1-156032177, pig14-83975934, pig10-62388272 site primer to mutation,
Meet Marker4, identifies that No. 5 pigs are Fengjing pig.
The related patents that do not identified both at home and abroad about Fengjing pig kind and its meat products kind (strain) at present, by the third generation
Molecular labeling applies to Fengjing pig and its meat products kind (strain) identification, has filled up the vacancy in market, has efficiently solved maple river rising in Ningxia and flowing into central Shaanxi
The false distinguishing problem of pig (strain).The identification method utilizes first generation molecular labeling (RFLP) and second generation molecule mark relative to existing
Note (SSR) does the patent of pig kind identification, has the advantages that operate simpler, result more accurately, rapidly and efficiently.This hair simultaneously
It is bright that the disadvantage that preceding two generations molecular labeling can be used site few, relatively preceding two generations molecule are overcome using third generation molecular marker SNP
For the authentication technique of label, discrimination method is also simplified.
Above-mentioned specific implementation can by those skilled in the art under the premise of without departing substantially from the principle of the invention and objective with difference
Mode carry out local directed complete set to it, protection scope of the present invention is subject to claims and not by above-mentioned specific implementation institute
Limit, each implementation within its scope is by the constraint of the present invention.
Sequence table
<110>Zhejiang University
<120>combination of the SNP marker of Fengjing pig and raw meat product and identification method
<160> 8
<170> SIPOSequenceListing 1.0
<210> 1
<211> 20
<212> DNA
<213>unknown (Unknown)
<400> 1
agctgtggct tgggttgtag 20
<210> 2
<211> 20
<212> DNA
<213>unknown (Unknown)
<400> 2
acagattcgg ctggacccta 20
<210> 3
<211> 20
<212> DNA
<213>unknown (Unknown)
<400> 3
tcttgcccct tatgctgtga 20
<210> 4
<211> 20
<212> DNA
<213>unknown (Unknown)
<400> 4
atcgctccaa accacagtcc 20
<210> 5
<211> 19
<212> DNA
<213>unknown (Unknown)
<400> 5
caaaagtgca aggggtccg 19
<210> 6
<211> 21
<212> DNA
<213>unknown (Unknown)
<400> 6
gaggccagat tatcactggg a 21
<210> 7
<211> 21
<212> DNA
<213>unknown (Unknown)
<400> 7
cacaacactg aggagtggtc a 21
<210> 8
<211> 20
<212> DNA
<213>unknown (Unknown)
<400> 8
cactgagcca cgacaggaac 20
Claims (5)
1. the SNP marker of a kind of Fengjing pig and raw meat product combines characterized by comprising pig17-12719441, pig1-
156032177, pig14-83975934 and pig10-62388272；The pig17-12719441 indicates No. 17 chromosomes of pig the
12719441 sites, pig1-156032177 indicate the 156032177th site of No. 1 chromosome of pig, pig14-83975934
Indicate the 83975934th site of No. 14 chromosomes of pig, pig10-62388272 indicates the 62388272nd position of No. 10 chromosomes of pig
Point.
2. a kind of Fengjing pig and its raw meat product identification method, feature based on the combination of SNP marker described in claim 1 exists
In, extract the genomic DNA of raw pork or meat products to be identified first, then after PCR amplification carry out Ago-Gel electricity
Swimming and Sanger sequencing, obtain the information that SNP marker combines each detection site, when SNP marker combines at any three sites
There is specific mutagenesis, Fengjing pig and its meat products can be accredited as.
3. according to the method described in claim 2, it is characterized in that, the PCR amplification, at pig17-12719441 up and down
Swim the sequence such as SEQ ID of upstream and downstream primer of the sequence of primer at the pig1-156032177 as shown in NO.1~2 SEQ ID
Shown in NO.3~4, the sequence of the upstream and downstream primer at pig14-83975934 is as shown in NO.5~6 SEQ ID, pig10-
The sequence of upstream and downstream primer at 62388272 is as shown in NO.7~8 SEQ ID.
4. according to the method described in claim 2, it is characterized in that, sequencing product identification site comparative information is as shown in the table:
REF, which is represented, in table refers to genotype, and ALT represents mutated-genotype.
5. according to the method described in claim 4, it is characterized in that, the identification site information are as follows: each detection site
Mutated-genotype thinks that meaning is not identified in the site when pig detection site to be measured occurs with reference to genotype with reference to genotype
Justice, when mutated-genotype occurs in pig detection site to be measured, it is believed that the site has Identification Significance.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910462663.8A CN110106260B (en) | 2019-05-30 | 2019-05-30 | SNP marker combination and identification method of Fengjing pigs and raw meat products |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910462663.8A CN110106260B (en) | 2019-05-30 | 2019-05-30 | SNP marker combination and identification method of Fengjing pigs and raw meat products |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110106260A true CN110106260A (en) | 2019-08-09 |
| CN110106260B CN110106260B (en) | 2020-12-08 |
Family
ID=67493046
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910462663.8A Active CN110106260B (en) | 2019-05-30 | 2019-05-30 | SNP marker combination and identification method of Fengjing pigs and raw meat products |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110106260B (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2002251326A1 (en) * | 2001-04-24 | 2002-11-05 | Pig Improvement Co (Uk) Ltd | Method to analyse the kit genotype of pigs |
| CN104164430A (en) * | 2014-08-21 | 2014-11-26 | 中国农业科学院北京畜牧兽医研究所 | Pork quality and meat yield related molecular markers and composite applications thereof |
| CN107299143A (en) * | 2017-08-03 | 2017-10-27 | 南京农业大学 | Pig No. 12 chromosome SNP markers related to Erhualian litter size and detection method |
| CN107699624A (en) * | 2017-10-25 | 2018-02-16 | 上海交通大学 | The SNP marker of little MeiShan pig and raw meat product combines and authentication method |
| KR101929391B1 (en) * | 2017-07-24 | 2018-12-17 | 대한민국 | Novel SNP marker for discriminating increasedthe number of nipples of pigs and use thereof |
-
2019
- 2019-05-30 CN CN201910462663.8A patent/CN110106260B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2002251326A1 (en) * | 2001-04-24 | 2002-11-05 | Pig Improvement Co (Uk) Ltd | Method to analyse the kit genotype of pigs |
| CN104164430A (en) * | 2014-08-21 | 2014-11-26 | 中国农业科学院北京畜牧兽医研究所 | Pork quality and meat yield related molecular markers and composite applications thereof |
| KR101929391B1 (en) * | 2017-07-24 | 2018-12-17 | 대한민국 | Novel SNP marker for discriminating increasedthe number of nipples of pigs and use thereof |
| CN107299143A (en) * | 2017-08-03 | 2017-10-27 | 南京农业大学 | Pig No. 12 chromosome SNP markers related to Erhualian litter size and detection method |
| CN107699624A (en) * | 2017-10-25 | 2018-02-16 | 上海交通大学 | The SNP marker of little MeiShan pig and raw meat product combines and authentication method |
Non-Patent Citations (3)
| Title |
|---|
| WANG, Z等: "Genetic diversity and population structure of six Chinese indigenouspig breeds in the Taihu Lake region revealed by sequencing data", 《ANIMAL GENETICS》 * |
| 王明庥: "《王明庥文集》", 31 January 2014 * |
| 高利华等: "3种猪孕酮受体基因(pgr)多态性分析", 《湖南农业大学学报(自然科学版)》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110106260B (en) | 2020-12-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110106261A (en) | The combination of the SNP marker of Jiaxing Black Pig and raw meat product and identification method | |
| CN110106262A (en) | The combination of the SNP marker of middle plum mountain pig and raw meat product and identification method | |
| CN107699624B (en) | SNP marker combination and identification method of small plum mountain pigs and raw meat products | |
| US11085075B2 (en) | DNA barcoding primer for identifying cephalopoda and use thereof | |
| Sasazaki et al. | Development of breed identification markers derived from AFLP in beef cattle | |
| CN105648046B (en) | Method for identifying sheep, goat, mink, nutria and duck meat at one time | |
| Abbadi et al. | Species identification of bivalve molluscs by pyrosequencing | |
| CN112899374A (en) | Molecular marker related to skin color of chicken, primer group and application | |
| CN110106263A (en) | The combination of the SNP marker of the white pig of Pudong and raw meat product and identification method | |
| CN110241226A (en) | The each kind of Taihu Lake basin local varieties pig and the combination of the SNP marker of raw meat product and identification method | |
| KR100898721B1 (en) | Method of identification between Hanwoo and foreign beef meats | |
| CN106119377A (en) | A kind of differentiate the primer of snapper, black porgy and filial generation thereof, test kit and discrimination method | |
| CN104152447A (en) | Single nucleotide polymorphism (SNP) molecular markers and method for pork DNA tracing by high resolution melting (HRM) method | |
| CN110157813A (en) | The combination of the SNP marker of husky rhizome of Chinese monkshood pig and raw meat product and identification method | |
| CN110144411A (en) | The combination of the SNP marker of rice pig and raw meat product and identification method | |
| Sasazaki et al. | Development of DNA markers for discrimination between domestic and imported beef | |
| CN110106260A (en) | The combination of the SNP marker of Fengjing pig and raw meat product and identification method | |
| CN110106259A (en) | The combination of the SNP marker of Erhualian and raw meat product and identification method | |
| CN101805800B (en) | Method for distinguishing indigenous pig breed in China from indigenous pig breed in Europe | |
| KR20160000975A (en) | Primer set for discrimination of Hanwoo and Methods discrimination of thereby | |
| CN109439764B (en) | Molecular marker for identifying native-like eggs and native eggs and application | |
| KR101321219B1 (en) | Diagnosis method of marbling heritability by genome analysis in Hanwoo | |
| CN108060239B (en) | Primer pair combination product, kit and method for distinguishing yak from non-yak | |
| Gupta et al. | Single-nucleotide primer extension assay of mtDNA to authenticate cattle and buffalo meat | |
| Sasazaki et al. | Breed discrimination using DNA markers derived from AFLP in Japanese beef cattle |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |