CN110079556A - A kind of black barley fermentation material is mitigating the application in injury of lungs and enteric flora disturbance - Google Patents
A kind of black barley fermentation material is mitigating the application in injury of lungs and enteric flora disturbance Download PDFInfo
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- CN110079556A CN110079556A CN201910334903.6A CN201910334903A CN110079556A CN 110079556 A CN110079556 A CN 110079556A CN 201910334903 A CN201910334903 A CN 201910334903A CN 110079556 A CN110079556 A CN 110079556A
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- injury
- lungs
- black barley
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Classifications
-
- A—HUMAN NECESSITIES
- A21—BAKING; EDIBLE DOUGHS
- A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
- A21D13/00—Finished or partly finished bakery products
- A21D13/06—Products with modified nutritive value, e.g. with modified starch content
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
- A23L2/382—Other non-alcoholic beverages fermented
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
- A23L7/10—Cereal-derived products
- A23L7/104—Fermentation of farinaceous cereal or cereal material; Addition of enzymes or microorganisms
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
- A61K36/8998—Hordeum (barley)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
- C12R2001/25—Lactobacillus plantarum
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicinal Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Veterinary Medicine (AREA)
- Polymers & Plastics (AREA)
- Genetics & Genomics (AREA)
- Mycology (AREA)
- General Chemical & Material Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Engineering & Computer Science (AREA)
- Medical Informatics (AREA)
- Alternative & Traditional Medicine (AREA)
- Botany (AREA)
- Pulmonology (AREA)
- Epidemiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of black barley fermentation materials to mitigate the application in injury of lungs and enteric flora disturbance, is related to medicine and health product technology field, and the fermenting microbe used of the fermentation material is lactobacillus plantarum.The present invention has the technical effect for mitigating stethemia situation and can improving enteric flora disturbance for mitigating injury of lungs caused by cooking fume and enteric flora disturbance.
Description
Technical field
The present invention relates to medicine and health product technology field more particularly to a kind of black barley fermentation material to mitigate caused by oil smoke
Injury of lungs and enteric flora disturbance in application.
Background technique
Lung cancer is an important global public health problem, it is characterized in that Delay in Diagnosis, early stage is shifted, high mortality and
Low survival rate.Cooking fume is one of indoor most common air pollution source, in the retrospective of developing country and rural area country
In case-control study, room air pollution (IAP) caused by cooking is related with lung-cancer-risk.In recent years, cooking fume exposure
Have proved to be one of pathogenic factor of lung cancer (Barko P C, Mcmichael M A, Swanson K S, et al.The
Gastrointestinal Microbiome:A Review[J].Journal of Veterinary Internal
Medicine,2018,32(1):9-25；Beamish L A,Osornio-Vargas AR,Wine E.Air pollution:
An environmental factor contributing to intestinal disease.[J].Journal of
Crohns&Colitis,2011, 5(4):279-286).In the prevalence that China's Mainland, TaiWan, China, Hong Kong and Singapore carry out
Disease is learned research shows that cooking fume (COF), which is exposed to, suffers from related (Denholm R, the Joachim Sch of lung cancer to non-smoking women
üz,Straif K,et al. Is previous respiratory disease a risk factor for lung
Cancer? [J] .Am J Respir Crit Care Med, 2014,190 (5): 549-559).
In addition, outdoor air pollution is also one of lung cancer morbidity factor.World Health Organization's estimation, 2012, city and rural area
Environment (outdoor) air pollution in area leads to 3,700,000 people's premature death of the whole world every year.Live in low income and medium income
The crowd of country is influenced to be particularly acute by outdoor air pollution: in 3,700,000 premature deaths, 88% occur in low income and
The burden of middle income country, Central West Pacific Ocean Region and south east asia is maximum, and (World Health Organization's environment (outdoor) is empty
Makings amount and health [EB/OL]
Http:// www.who.int/mediacentre/factsheets/fs313/zh/, 2014-3/2016-6-4),
And China is the severely afflicated area of air pollution.
The polycyclic aromatic hydrocarbon (PAHs) that contains in cooking fume, aldehydes, volatile organic compounds, (diameter is small for ultra-fine grain
In the particle of 100nm) and PM2.5's (substance of the particulate matter diameter less than 2.5 microns) and the PM2.5 in pollution air is most straight
Connecing effect target organ is respiratory system (Guarnieri M, Balmes J R.Outdoor air pollution and
asthma[J].Food&Chemical Toxicology An International Journal Published for the
British Industrial Biological Research Association,2014,383(9928):1581-1592；
He Y,Wen Q,Yao F,et al.Gut–lung axis:The microbial contributions and clinical
implications[J]. Critical Reviews in Microbiology,2016:1-15；Laurentbabot C,
Guyot J P.Should Research on the Nutritional Potential and Health Benefits of
Fermented Cereals Focus More on the General Health Status of Populations in
Developing Countries? [J] .Microorganisms, 2017,5 (3): 40).
And lungs are one of important components of respiratory system.Lung function is the morning of heart and lung diseases disease incidence, the death rate
The important indicator of phase, objective and high quality prediction index and air pollutants exposure, air pollution is to the acute of lung function
Influence is often referred to after exposure occurs a few hours to a couple of days, and current studying is strong after discovery air pollution exposure exposes it one day
Health Outcome maximum (Lee C L, Pan T M.Red mold fermented products and Alzheimer ' s
disease:a review[J].Applied Microbiology and Biotechnology,2011,91 (3):461-
469), Chronic Effect usually continues the several years.Therefore, by Dietary frequency, prevent or alleviate air pollution to lead to early stage injury of lungs
It has very important significance and feasibility.
Environmental pollution has close be associated with the change of intestinal flora.Room, inside and outside pollution air in containing fine
Particle, it is previous studies have shown that sucking particulate matter (PM) will lead to decline in pulmonary function (Lippmann M, Schlesinger
R B.Interspecies comparisons of particle deposition and mucociliary clearance
in tracheobronchial airways[J].Journal of Toxicology and Environmental
Health,1984,13 (2-3):441-469).Particulate air pollution cause injury of lungs studies have shown that the particle of smaller size,
Especially particle (PM2.5) of the average diameter less than 2.5 microns can reach bronchiole and alveolar space, there their quilts
Pulmonary alveolar macrophage swallows (Miller F J.Dosimetry of Particles:Critical Factors Having
Risk Assessment Implications[J].Inhalation Toxicology,2000,12(sup3):7；Moller,
W. Mucociliary and long-term particle clearance in the airways of healthy
nonsmoker subjects[J].Journal of Applied Physiology,2004,97(6):2200-2206；Mu
L,Liu L,Niu R, et al.Indoor air pollution and risk of lung cancer among
Chinese female non-smokers[J]. Cancer Causes&Control,2013,24(3):439-450).Huge
It is isolated in phagocyte and the particle directly in the rete malpighii of lower respiratory tract is then transported to pars oralis pharyngis, then swallows stomach and intestine
(GI) road (Laura P, Nadia H. Particulate air pollution and impaired lung function
[J].F1000Research,2016,5:201-.).(Mutlu E A, Comba I Y, the Cho T, et such as Mutlu E A
al.Inhalational exposure to particulate matter air pollution alters the
composition of the gut microbiome[J].Environmental Pollution,2018,240:817-
830) research discovery, which is exposed to PM, can lead to mouse intestinal flora imbalance.Intestinal flora it is normal when can by building mechanical barrier,
Biological barrier and immunization barrier resist the invasion of external pathogenic microorganism, maintain the stabilization and microecological balance of intestinal environment.
And intestinal bacilli illness then can lead to a variety of diseases such as inflammatory bowel disease, obesity, cardiovascular disease etc..
Research finds enteric flora disturbance and closely related (Singer B C, the Delp W W.Response of lung cancer morbidity
of consumer and research grade indoor air quality monitors to residential
sources of fine particles[J].Indoor Air,2018).Therefore, a certain amount of diet is taken in by Dietary frequency
Replenishers, to adjust and improve the enteric flora disturbance caused by polluting air because of sucking indoor and outdoor, to prevent because continuing lung
A series of diseases (such as lung cancer) that damage causes have important practical significance and feasibility.
Fermented food can not only enrich human diet nutrition, provide satisfactory taste, fragrance and quality.Can also it pass through
Microbial fermentation generates benefit materials and probiotics, removes antinutriment, antinutritional factor.Studying more about fermented cereal at present is brown rice,
Its biological activity is mainly used for the prevention of cardiovascular disease, tumour etc..However, strain and fermented cereal are different, fermentation material
Nutritive peculiarity and bioactivity it is different.
Therefore, those skilled in the art is dedicated to developing a kind of black barley fermentation material and is mitigating injury of lungs and intestinal flora
Application in disorder prevents using black barley fermentation material and mitigates oil smoke Lung Injury and enteric flora disturbance.
Summary of the invention
In view of the above drawbacks of the prior art, the technical problem to be solved by the present invention is to how utilize the fermentation of black barley
Object provides a kind of new application: a kind of black barley fermentation material is preventing and is correcting cooking fume and air pollution Lung Injury and intestines
The application of road Flora Disturbance.
To achieve the above object, the present invention provides a kind of black barley fermentation materials to mitigate injury of lungs and enteric flora disturbance
In application, fermenting microbe used in the fermentation material is lactobacillus plantarum (Lactobacillus kisonensis).
Preferably, the injury of lungs and enteric flora disturbance are caused by air pollution.
Preferably, the air pollution is cooking fume pollution.
Preferably, the fermentation material be made product be black barley lactobacillus ferment food, black barley lactobacillus ferment drink,
Solid powdery function obtained is processed into the drying of black barley lactobacillus ferment object using freeze-drying or spray drying processing
Property food.
Preferably, the application method of the fermentation material includes directly eating the fermentation material, utilizing freeze-drying or spraying
Dry processing is eaten after the dry processing of the fermentation material is obtained solid powdery functional food, or with the fermentation material is main
The compatibility functional food of ingredient.
The present invention provides a kind of methods of mitigation injury of lungs and enteric flora disturbance, take orally black barley fermentation material and its hair
A kind of combination of one-component of yeast-like fungi kind or both, the fermenting microbe are lactobacillus plantarum.
The present invention provides a kind of mitigation injury of lungs and the product of enteric flora disturbance, the active constituent of the product is black
A kind of combination of one-component of barley fermentation material and its fermenting microbe or both, the fermenting microbe are lactobacillus plantarum.
Preferably, the product is functional food or drug, and the application method of the product is oral.
Preferably, the functional food includes drink or bakes based food, the dosage form of the drug be capsule, tablet,
Pill, granule or suspension.
The preparation method that the present invention provides a kind of for mitigating the fermentation material of injury of lungs and enteric flora disturbance include with
Lower step: (1) will cross 80 meshes after black barley pulverization, be that pure water is added in 1:5 solid-liquid ratio according to mass ratio, agitating and heating is warming up to
65℃；(2) α-alpha-amylase is added, additive amount 10U/g is digested 40 minutes, and black barley culture medium is obtained after the completion of enzymatic hydrolysis；
(3) the black barley culture medium is put into high-pressure sterilizing pot sterilizing, it is cooling after by 3% inoculum concentration to the black barley after cooling
Activated fermenting microbe bacterium solution is added in culture medium, contains viable count 109CFU/ml ferments 24 hours at 30 DEG C, and revolving speed is
100 revs/min of shaker fermentations；The fermenting microbe is lactobacillus plantarum.
Black barley lactobacillus ferment object provided by the invention causes stethemia situation with can significantly mitigate cooking fume, and can
Improve the technical effect of the disorder of intestinal flora caused by cooking fume.Terminate with up to experiment, black barley cream provided by the invention
Bacillus fermentation object has no adverse effects to various aspects such as growth of animal.Therefore black barley lactobacillus ferment object provided by the invention can be made
For the functional food for preventing and mitigating cooking fume Lung Injury and enteric flora disturbance, or the master with Related Component compatibility
Active constituent is wanted, in conjunction with environmental pollution states instantly, is had a good application prospect.
It is described further below with reference to technical effect of the attached drawing to design of the invention, specific structure and generation, with
It is fully understood from the purpose of the present invention, feature and effect.
Detailed description of the invention
Fig. 1 is the influence that black barley lactobacillus ferment object accounts for body ratio to 4 kinds of internal organs of mouse after cooking fume processing；
Fig. 2 is each group mouse food ration situation of change in experimental period；
Fig. 3 is each group mouse weight situation of change in experimental period；
Fig. 4 is mouse lung tissue HE coloring pathological section；
Fig. 5 is point of 4 species diversity indexes of influence of the black barley lactobacillus ferment object to mouse intestinal flora alpha diversity
Analyse result；
Fig. 6 is the offset minimum binary differentiation point of influence of the black barley lactobacillus ferment object to mouse intestinal flora beta diversity
It analyses (PLS-DA) and analyzes result；
Fig. 7 be black barley lactobacillus ferment object to mouse intestinal flora 6 kinds of flora distribution situations of door level shadow
It rings；
Fig. 8 is influence of the black barley lactobacillus ferment object to antioxidation activity in vitro；
Fig. 9 is influence of the black barley lactobacillus ferment object to antioxidant status in Mice Body.
Specific embodiment
Multiple preferred embodiments of the invention are introduced below with reference to Figure of description, keep its technology contents more clear and just
In understanding.The present invention can be emerged from by many various forms of embodiments, and protection scope of the present invention not only limits
The embodiment that Yu Wenzhong is mentioned.
In the accompanying drawings, the identical component of structure is indicated with same numbers label, everywhere the similar component of structure or function with
Like numeral label indicates.The size and thickness of each component shown in the drawings are to be arbitrarily shown, and there is no limit by the present invention
The size and thickness of each component.Apparent in order to make to illustrate, some places suitably exaggerate the thickness of component in attached drawing.
Embodiment 1:
The preparation of black barley lactobacillus ferment object:
80 meshes will be crossed after black barley pulverization, according to 1:5 (m/m) solid-liquid ratio, pure water is added, agitating and heating is warming up to 65
℃；α-alpha-amylase (10U/g) is added and digests 40min；Black barley culture medium after the completion of saccharification is put into high-pressure sterilizing pot and goes out
Activated lactobacillus plantarum bacterium solution is added into black barley culture medium after cooling by 3% inoculum concentration after cooling and (contains and lives for bacterium
Bacterium number 109CFU/ml), it ferments for 24 hours at 30 DEG C, shaker fermentation (100rpm).
Black barley lactobacillus ferment object and its fermenting microbe are as mitigation oil smoke and air pollution Lung Injury and enteron aisle
The health care product (functional food) of Flora Disturbance, the following are Pharmacodynamic test of active extract:
1, black barley lactobacillus ferment object antioxidation activity in vitro analysis:
Total antioxidant capacity (T-AOC) builds up biological Co., Ltd's kit A015-3 (FRAP method) using Nanjing；Hydroxyl is certainly
The kit A018 (Fenton microplate method) of biological Co., Ltd is built up using Nanjing by base Scavenging activity；DPPH free radical is clear
Except rate detects as follows: 0.5mL sample (blank is replaced with 80% ethyl alcohol) is added in 4mL 2 × 10-4The DPPH of mol/L
In solution, shakes up and place 30min at room temperature；Its light absorption value Ai is measured under 517nm；4mL 2 × 10 is measured simultaneously-4mol/
The light absorption value Ac of LDPPH solution and 0.5mL dehydrated alcohol mixed liquor；0.5mL sample liquid and 4mL dehydrated alcohol mixed liquor are surveyed again
Light absorption value Aj, calculate sample according to the following formula to the clearance rate of DPPH: clearance rate (%)=[1- (Ai-Aj)/Ac] ×
100。
2, zoopery interference method and index analysis:
In order to observe black barley lactobacillus ferment object to mitigation cooking fume and air pollution Lung Injury and enterobacteriaceae
Function and effect in group's disorder, this test utilize 8 week old ICR male mices (25-30g), and adaptable fed after a week, will test
Animal is divided into: normal group, fermentation liquor treatment group, oil smoke group, oil smoke fermentation liquor treatment group, oil smoke lactic acid bacteria processing group, and every group 5
Only.
Oil smoke processing processing: self-control oil smoke treatment box is connected with hose with oil cauldron pot mouth, oil smoke is passed through oil smoke by hose
Treatment box, each oil smoke processing group are that edible blend oil is heated to 200-220 DEG C of oil temperature (400 DEG C of mercurial thermometers control temperature)
When oil smoke will be needed to handle same group of mouse be put into oil smoke processing simultaneously, each oil smoke processing time is 5min, 4 times/week, oily
Cigarette is handled total duration 9 weeks.Mouse weight is recorded during experiment weekly, records food ration situation daily.
Nutritional agents stomach-filling: (the coated plate counting of viable bacteria amount is maintained at 10 for lactic acid bacteria and fermentation liquid8CFU/mL) stomach-filling amount is equal
According to 1mL/100g weight (each 350 μ L of stomach-filling), daily stomach-filling 1 time, continuous gavage 9 weeks.Cervical vertebra after being anaesthetized at the end of experiment
Mouse is put to death in dislocation, and the tissues such as lungs is taken to do following analysis detection.
1) paragonimus cyst, liver index and spleen index weigh weight before mouse is put to death.Lung, the liver of mouse are carefully won when execution
Dirty and spleen is cleaned with physiological saline, draws excessive moisture, each histoorgan quality of precise with clean filter paper.Lung system
Number (%)=lung quality/mouse quality * 100；Liver index (%)=liver quality/mouse quality * 100；Spleen index (%)=spleen matter
Amount/mouse quality * 100；
2) production of pathologic slice, takes fresh liver tissue to fix, paraffin embedding, makes pathology using HE decoration method
Slice, om observation, amplification factor are 400 times；
3) analysis of intestinal microflora；
4) black barley lactobacillus ferment object antioxidation activity in vitro analysis:
FRAP: total antioxidant capacity (T-AOC) kit A015-3FRAP method of biological Co., Ltd is built up using Nanjing
Measurement；
Hydroxyl radical free radical: scavenging hydroxyl ability builds up the kit A018 of biological Co., Ltd using Nanjing
(Fenton microplate method)；
DPPH clearance rate: accurately weighing 7.88mgDPPH, is dissolved with dehydrated alcohol, and constant volume shakes in 100mL volumetric flask
Even, compound concentration is 2 × 10-4The solution of mol/L is stored in spare in refrigerator.To 4mL 2 × 10-4The DPPH of mol/L is molten
0.5mL sample (blank is replaced with 80% ethyl alcohol) is added in liquid, shakes up and places 30min at room temperature, make reference with dehydrated alcohol
Liquid measures its light absorption value Ai under 517nm；4mL 2 × 10 is measured simultaneously-4Mol/LDPPH solution and 0.5mL dehydrated alcohol are mixed
Close the light absorption value Ac of liquid；The light absorption value Aj for surveying 0.5mL sample liquid Yu 4mL dehydrated alcohol mixed liquor again, calculates according to the following formula
Clearance rate of the sample to DPPH: clearance rate (%)=[1- (Ai-Aj)/Ac] × 100；
5) antioxidant activity is analyzed in black barley lactobacillus ferment object.Mouse lung tissue SOD (A001-3) and CAT
(A007-1-1) activity analysis is all made of the kit that biological Co., Ltd is built up in Nanjing.
3, statistical procedures:
Experimental result indicates that statistical analysis software is SPSS19.0 (IBM, beauty with mean+SD (mean ± SD)
State), comparison among groups are carried out using one-way ANOVA, P < 0.05 is that difference is statistically significant.
The experimental results showed that black barley lactobacillus ferment object of the invention, which can significantly mitigate cooking fume, causes stethemia situation,
And the disorder of intestinal flora caused by cooking fume can be improved, until experiment terminates to have no adverse effects to various aspects such as growth of animal.It is real
Result is tested to be described below:
As shown in Figure 1, influence of the black barley lactobacillus ferment object to mouse lung body ratio after cooking fume processing.When generation lung
When oedema, paragonimus cyst can be significantly increased.The discovery of this experimental result, oil smoke group mouse lung body ratio is significantly higher than normal group, and oil smoke
Fermentation liquor treatment group then with normally organize close, it is swollen to show that black barley lactobacillus ferment liquid can reduce stethemia caused by cooking fume
Greatly；Each group mouse liver index, spleen index and renal index prompt the safety of black barley fermentation liquid without significant difference, to mouse its
Hetero-organization organ is without influence.
As shown in Figures 2 and 3, each group mouse food ration and changes of weight situation in experimental period.Normal mouse stomach-filling is given
Give black barley lactobacillus ferment liquid food ration and body weights always with normally organize it is close；And each cooking fume processing group is in reality
It tests initial stage, i.e., the 5th day to the 25th day food ration and weight loss, this is mainly that oil smoke processing leads to mouse loss of appetite, and with
The extension of oil smoke process cycle, the food ration and weight of mouse be gradually recovered normally, each group is without significant difference.Show black barley
Fermentation liquid does not make significant difference to the diet and weight of mouse, further prompts the safety of black barley fermentation liquid.
As shown in figure 4, pathological effect of the black barley lactobacillus ferment object to cooking fume induced mice injury of lungs.For into
One step observes influence of the black barley lactobacillus ferment object to cooking fume processing mouse early stage injury of lungs, and experiment is dyed using HE
Observe each group mouse lung pathological change situation.The alveolar structure of normal group mouse is normal, the expansion of part alveolar space, ramuscule gas
There are a small amount of bleeding and slight lymph-plasmocyte infiltrating in pipe；And oil smoke group male mice diffusivity bronchiole, breathing and lung
It steeps intracavitary cellulose exudation and extent of hemorrhage is most heavy, the infiltration of interstitial moderate lymphoplasmacytic, air cavity partial compression is narrow；Fermentation liquid
Processing group, oil smoke fermentation liquor treatment group and oil smoke lactic acid bacteria processing group mouse above situation are substantially reduced, show as alveolar cavity portion
Divide expansion, has a small amount of bleeding and slight lymph-plasmocyte infiltrating in bronchiole, it is poly- around bronchiole wall local lymphoid tissue
Collection hyperplasia there is lymph follicle to be formed, part air cavity be pressurized and it is narrow.From further confirming that stomach-filling is edible in the present invention on morphology
Black barley lactobacillus ferment object can effectively mitigate cooking fume induced mice injury of lungs.
As shown in Fig. 5, Fig. 6 and Fig. 7, influence of the black barley lactobacillus ferment object to mouse intestinal flora.From alpha diversity point
It can be seen that oil smoke group mouse Chao index, ACE index, Simpson index, Shannon index decline in analysis, show oil smoke
Processing can make the diversity and abundance decline of mouse intestinal flora；And black barley lactobacillus ferment object can reduce this trend,
Above-mentioned index with normally organize it is closer；Beta diversity also demonstrates this point, analyzes from partial least squares discriminant analysis (PLS-DA)
As a result it will be evident that oil smoke fermentation liquor treatment group and the overall abundance of oil smoke lactic acid bacteria processing group intestinal flora are organized more with normal
It is close.In the higher flora of 6 wealth of species (Firmicutes, Bacteroidetes, wart germ door, Proteobacteria, actinomyces door, TM7
Bacterium door) flora door level, oil smoke fermentation liquor treatment group and the relative abundance of oil smoke lactic acid bacteria processing group intestinal flora and normal group
It is closer, show that black barley lactobacillus ferment object and lactobacillus used herein can effectively correct enterobacteriaceae caused by cooking fume
The disorder of group.
As can be seen from figures 8 and 9, black barley lactobacillus ferment object is to antioxidation activity in vitro and to shape anti-oxidant in Mice Body
The influence of state.The oxidation that will cause body in view of containing the nuisances such as more peroxide and polycyclic aromatic hydrocarbon in oil smoke smog is answered
Swash state, the present invention also analyzes the inside and outside antioxidant activity of body of black barley lactobacillus ferment object.As can be seen from the figure this hair
Bright black barley lactobacillus ferment object used can effectively scavenging hydroxyl, DPPH free radical, improve total antioxidant capacity；Significantly mention
The activity of high mouse lung tissue superoxide dismutase (SOD)；In addition, mouse lung tissue catalase after oil smoke processing
(CAT) it is substantially less than normal group, and oil smoke fermentation liquor treatment group mouse CAT activity is slightly higher, and with normal group without significant difference,
Show that black barley lactobacillus ferment object makes moderate progress to the oxidative stress status of oil smoke induced mice.
Embodiment 2:
On the basis of embodiment 1, black barley lactobacillus is further made in the black barley lactobacillus ferment object of acquisition of fermenting
Fermented food, black barley lactobacillus ferment drink are processed using freeze-drying or spray drying by black barley lactobacillus ferment object
It is dry to process solid powdery functional food obtained.
The preferred embodiment of the present invention has been described in detail above.It should be appreciated that the ordinary skill of this field is without wound
The property made labour, which according to the present invention can conceive, makes many modifications and variations.Therefore, all technician in the art
Pass through the available technology of logical analysis, reasoning, or a limited experiment on the basis of existing technology under this invention's idea
Scheme, all should be within the scope of protection determined by the claims.
Claims (10)
1. a kind of black barley fermentation material is mitigating the application in injury of lungs and enteric flora disturbance, which is characterized in that the fermentation
Fermenting microbe used in object is lactobacillus plantarum (Lactobacillus kisonensis).
2. black barley fermentation material as described in claim 1 is mitigating the application in injury of lungs and enteric flora disturbance, feature
It is, the injury of lungs and enteric flora disturbance are caused by air pollution.
3. black barley fermentation material as claimed in claim 2 is mitigating the application in injury of lungs and enteric flora disturbance, feature
It is, the air pollution is cooking fume pollution.
4. black barley fermentation material as described in claim 1 is mitigating the application in injury of lungs and enteric flora disturbance, feature
It is, the fermentation material is made product and is done for black barley lactobacillus ferment food, black barley lactobacillus ferment drink, using freezing
The drying of black barley lactobacillus ferment object is processed solid powdery functional food obtained by dry or spray drying processing.
5. black barley fermentation material as described in claim 1 is mitigating the application in injury of lungs and enteric flora disturbance, feature
Be, the application method of the fermentation material include directly eat the fermentation material, will using freeze-drying or spray drying processing
The dry processing of the fermentation material is eaten after obtaining solid powdery functional food, or with fermentation material compatibility as main component
Functional food.
6. a kind of method for mitigating injury of lungs and enteric flora disturbance, which is characterized in that take orally black barley fermentation material and its fermentation
A kind of combination of one-component of strain or both, the fermenting microbe are lactobacillus plantarum.
7. a kind of product for mitigating injury of lungs and enteric flora disturbance, which is characterized in that the active constituent of the product is black big
A kind of combination of one-component of wheat fermentation material and its fermenting microbe or both, the fermenting microbe are lactobacillus plantarum.
8. mitigating the product of injury of lungs and enteric flora disturbance as claimed in claim 7, which is characterized in that the product is function
Energy property food or drug, the application method of the product are oral.
9. mitigating the product of injury of lungs and enteric flora disturbance as claimed in claim 8, which is characterized in that the functional food
Product include drink or bake based food, and the dosage form of the drug is capsule, tablet, pill, granule or suspension.
10. a kind of for mitigating the preparation method of the fermentation material of injury of lungs and enteric flora disturbance, which is characterized in that including following
Step: (1) will cross 80 meshes after black barley pulverization, be that pure water is added in 1:5 solid-liquid ratio according to mass ratio, agitating and heating is warming up to 65
℃；(2) α-alpha-amylase is added, additive amount 10U/g is digested 40 minutes, and black barley culture medium is obtained after the completion of enzymatic hydrolysis；
(3) the black barley culture medium is put into high-pressure sterilizing pot sterilizing, it is cooling after by 3% inoculum concentration to the black barley after cooling
Activated fermenting microbe bacterium solution is added in culture medium, contains viable count 109CFU/ml ferments 24 hours at 30 DEG C, revolving speed 100
Rev/min shaker fermentation；The fermenting microbe is lactobacillus plantarum.
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