CN110075127A - A kind of concocting method of hiruto - Google Patents
A kind of concocting method of hiruto Download PDFInfo
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- CN110075127A CN110075127A CN201910388308.0A CN201910388308A CN110075127A CN 110075127 A CN110075127 A CN 110075127A CN 201910388308 A CN201910388308 A CN 201910388308A CN 110075127 A CN110075127 A CN 110075127A
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- hiruto
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/62—Leeches; Worms, e.g. cestodes, tapeworms, nematodes, roundworms, earth worms, ascarids, filarias, hookworms, trichinella or taenia
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F26—DRYING
- F26B—DRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM
- F26B5/00—Drying solid materials or objects by processes not involving the application of heat
- F26B5/04—Drying solid materials or objects by processes not involving the application of heat by evaporation or sublimation of moisture under reduced pressure, e.g. in a vacuum
- F26B5/06—Drying solid materials or objects by processes not involving the application of heat by evaporation or sublimation of moisture under reduced pressure, e.g. in a vacuum the process involving freezing
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Abstract
The invention discloses a kind of concocting methods of hiruto, specifically clean hiruto, clean and dry, and the water of hiruto weight percent 2~5% is then added, and then freeze 3h~5h at -20 DEG C of temperature and obtain material a to icing；Material a is beaten with beater and is slurried to obtain material b；Material b is placed in freeze-drying disk, with the dry 40h~60h of -50 DEG C~45 DEG C progress gradient freezings of temperature, obtains hiruto freeze-dried powder c by condensation dehydration；Hiruto freeze-dried powder c progress low-temperature grinding is sieved to obtain the hiruto after object processes.The present invention solves the problems, such as the reduction of high temperature antithrombin activity; it remains in hiruto body simultaneously and deposits blood; avoid deposited because hiruto is intracorporal blood be allowed to dry cause antithrombin activity reduce problem; the active constituent in hiruto can be retained to greatest extent; concocting process is easy, working condition is easily manipulated; product quality controllability is good, is suitble to large-scale production and popularization.
Description
Technical field
The invention belongs to medicinal material processing technical fields, and in particular to a kind of concocting method of hiruto.
Background technique
Hiruto (Poecilobdella manillensis) is a kind of hirudinaria manillensis, is commonly called as golden-rimmed leech, be Hirudinea without
The dry entirety of Rhynchobdellida Yi Zhi section animal hiruto, is Chinese medicine traditional simply, first recorded in Shennong's Herbal." name doctor is other
Record ", " Variorum of Shennong ", " Bencao Tujing ", Tang Materia Medica, the history trees such as Compendium of Material Medica it is on the books." Tang's sheet
Grass " cloud " has leech, careless leech.The big long ruler of person is permitted, the person that has blood in abdomen, and dry is preferred."
Hiruto is distributed mainly on the provinces and regions such as Guangxi, Guangdong, Hainan and Fujian, to suck people, animal, batrachia under natural environment
Equal vertebrates blood is made a living.Hiruto has the pharmacological activity such as significant anticoagulation, antithrombotic, thrombolysis, platelet aggregation-against,
Main chemical compositions be fatty acid, amino acid and protein, wherein reported active constituent be hirudin P6, hirudin P18,
Bufrudin A and Bufrudin B.Acute toxicity test shows that hiruto does not have apparent toxic side effect, therefore it has significantly
Medical value.
Hirudin (hirudin) is one of the salivary gland for being present in hirudinaria manillensis hiruto (golden-rimmed leech) albumen
Matter, the antithrombin activity with high special inhibit fibrin ferment bound substrates, are strongest fibrin ferments found so far
Natural specific inhibitor.Modern study also confirms that, with " Chinese Pharmacopoeia " non-bloodsucking variety protection, the hiruto sucked blood is (golden-rimmed
Leech) in contain more abundant hirudin, anticoagulating active is also higher compared with other nonbloodsucking class leech.Currently, hiruto is as body
High one of the leech kind of interior hirudin comparision contents, clinic be usually used in treat cephalophyma, brain edema, subcutaneous hematoma, cerebral thrombosis,
Cerebral hemorrhage, high sticky blood, high blood pressure, coronary heart diseases and angina pectoris are the fiest-tire medications for treating cerebral apoplexy, cerebral apoplexy sequela.Closely
It is more well sold and in short supply on the market over year, and mostly sold with living body and Frozen Body.
The concocting method of the hiruto of the prior art is recorded in " strengthening medicine quality standard in Guangxi Zhuang Autonomous Region " (volume Two)
DYB45-GXZYC0117-2011 captures hiruto in summer, two season of autumn, cleans, scalded extremely with boiling water, dry or low temperature is dry
It is dry.If will cause the antithrombin activity of hiruto if hiruto is directly scalded dead and not crowded blood convection drying with boiling water
I.e. hirudin substantially reduces, and then directly affects the curative effect of hiruto.
For hiruto mostly with folk prescription medication, clinic shows that raw effect is best, but Most patients reflection is extremely disliked when taking
The heart, or even vomiting phenomenon is generated, the medication impression of patient is not only affected, curative effect is more reduced.Present inventor is through grinding
Study carefully discovery, the activity of the intracorporal antithrombase of hiruto is influenced by temperature and whether deposit blood in vivo.If living body hiruto is straight
It connects and scalds extremely, because temperature is higher intracorporal antithrombin activity can reduce；If the intracorporal blood of depositing of hiruto after death is not put
It is dry, can also antithrombin activity be made to reduce；Can also antithrombin activity be made to reduce if beating powder by low temperature drying room temperature.
To sum up, the concocting method of the hiruto of the prior art has the drawback that: hiruto scald it is dead, deposit blood, xeraphium
In the process such as broken, its effective component i.e. hirudin is caused to substantially reduce, and then directly affects the curative effect of hiruto.Therefore,
It is urgent to provide a kind of concocting methods of new hiruto at present, so as to solve the deficiencies in the prior art.
Summary of the invention
The purpose of the present invention is to provide a kind of concocting methods of hiruto.
The object of the present invention is achieved like this, the concocting method of the hiruto the following steps are included:
1) hiruto is cleaned, clean dry, then be added hiruto weight percent 2~5% water, then in
3h~5h is freezed at -20 DEG C of temperature obtains material a to icing；
2) material a is beaten with beater and is slurried to obtain material b；
3) material b is placed in freeze-drying disk, dries 40h~60h with -50 DEG C~45 DEG C progress gradient freezings of temperature,
Hiruto freeze-dried powder c is obtained by condensation dehydration；
4) hiruto freeze-dried powder c progress low-temperature grinding is sieved to obtain the hiruto after object processes.
The present invention solves the problems, such as the reduction of high temperature antithrombin activity, while remaining in hiruto body and depositing blood, avoids
Blood is deposited because hiruto is intracorporal and is allowed to dry the problem for causing antithrombin activity to reduce, and can be retained in hiruto to greatest extent
Active constituent.Concocting process of the invention is easy, working condition is easily manipulated, and product quality controllability is good, and product quality is steady
It is fixed, it is suitble to large-scale production and popularization.
Specific embodiment
Below with reference to embodiment, the present invention is further illustrated, but the present invention is limited in any way,
Based on present invention teach that it is made it is any transform or replace, all belong to the scope of protection of the present invention.
The concocting method of hiruto of the present invention the following steps are included:
1) hiruto is cleaned, clean dry, then be added hiruto weight percent 2~5% water, then in
3h~5h is freezed at -20 DEG C of temperature obtains material a to icing；
2) material a is beaten with beater and is slurried to obtain material b；
3) material b is placed in freeze-drying disk, dries 40h~60h with -50 DEG C~45 DEG C progress gradient freezings of temperature,
Hiruto freeze-dried powder c is obtained by condensation dehydration；
4) hiruto freeze-dried powder c progress low-temperature grinding is sieved to obtain the hiruto after object processes.
The cleaning is to carry out spray washing to hiruto with flowing pure water.
The gradient freezing drying is from cold stage gradually to the drying process of hot stage.
The gradient freezing dry gradient temperature stage is 12 temperature stages.
12 temperature stages are -50 DEG C~-40 DEG C, -40 DEG C~-35 DEG C, -35 DEG C~-20 DEG C, -20 DEG C~-15
DEG C, -15 DEG C~-5 DEG C, -5 DEG C~0 DEG C, 0 DEG C~8 DEG C, 8 DEG C~15 DEG C, 15 DEG C~25 DEG C, 25 DEG C~30 DEG C, 30 DEG C~35 DEG C,
35 DEG C~45 DEG C.
Described -50 DEG C~-40 DEG C of temperature stages drying time is 5~10h.
Described -40 DEG C~-35 DEG C, -35 DEG C~-20 DEG C, -20 DEG C~-15 DEG C, -15 DEG C~-5 DEG C, -5 DEG C~0 DEG C, 0
DEG C~drying times of 8 DEG C, 8 DEG C~15 DEG C, 15 DEG C~25 DEG C, 25 DEG C~30 DEG C and 30 DEG C~35 DEG C of temperature stages is respectively 3
~6h.
The drying time of 35 DEG C~-45 DEG C temperature stages is 5~10h.
The low-temperature grinding is crushed at 2~10 DEG C of temperature.
The low-temperature grinding sieving is that low-temperature grinding crosses 50 meshes.
Embodiment 1
Hiruto is subjected to spray washing with flowing pure water, cleans and dries, hiruto weight percent 2% is then added
Water, then freeze 3h at -20 DEG C of temperature and obtain material a to icing；Material a is beaten with beater and is slurried to obtain material b；
Material b is placed in freeze-drying disk, it is dry with -50 DEG C~45 DEG C progress gradient freezings of temperature, phenanthrene is obtained by condensation dehydration
Ox leech freeze-dried powder c；Hiruto freeze-dried powder c is subjected to low-temperature grinding at 10 DEG C and crosses the luxuriant and rich with fragrance ox after 50 meshes obtain object processing
Leech.The gradient freezing dry gradient temperature stage is 12 temperature stages, specially -50 DEG C~-40 DEG C temperature stage 5h, -40
DEG C~-35 DEG C of temperature stage 3h, -35 DEG C~-20 DEG C temperature stage 6h, -20 DEG C~-15 DEG C temperature stage 3h, -15 DEG C~-5 DEG C
Temperature stage 3h, -5 DEG C~0 DEG C temperature stage 3h, 0 DEG C~8 DEG C temperature stage 6h, 8 DEG C~15 DEG C temperature stage 6h, 15 DEG C~
25 DEG C of temperature stage 3h, 25 DEG C~30 DEG C temperature stage 3h, 30 DEG C~35 DEG C temperature stage 3h, 35 DEG C~45 DEG C temperature stages
5h。
Embodiment 2
Hiruto is subjected to spray washing with flowing pure water, cleans and dries, hiruto weight percent 5% is then added
Water, then freeze 5h at -20 DEG C of temperature and obtain material a to icing；Material a is beaten with beater and is slurried to obtain material b；
Material b is placed in freeze-drying disk, it is dry with -50 DEG C~45 DEG C progress gradient freezings of temperature, phenanthrene is obtained by condensation dehydration
Ox leech freeze-dried powder c；Hiruto freeze-dried powder c is subjected to low-temperature grinding at 2 DEG C and crosses the hiruto after 50 meshes obtain object processing.
The gradient freezing dry gradient temperature stage is 12 temperature stages, specially -50 DEG C~-40 DEG C temperature stage 10h, -40 DEG C
~-35 DEG C of temperature stage 3h, -35 DEG C~-20 DEG C temperature stage 3h, -20 DEG C~-15 DEG C temperature stage 6h, -15 DEG C~-5 DEG C temperature
Spend stage 6h, -5 DEG C~0 DEG C temperature stage 3h, 0 DEG C~8 DEG C temperature stage 3h, 8 DEG C~15 DEG C temperature stage 6h, 15 DEG C~25
DEG C temperature stage 3h, 25 DEG C~30 DEG C temperature stage 3h, 30 DEG C~35 DEG C temperature stage 6h, 35 DEG C~45 DEG C temperature stage 10h.
Embodiment 3
Hiruto is subjected to spray washing with flowing pure water, cleans and dries, hiruto weight percent 3% is then added
Water, then freeze 4h at -20 DEG C of temperature and obtain material a to icing；Material a is beaten with beater and is slurried to obtain material b；
Material b is placed in freeze-drying disk, it is dry with -50 DEG C~45 DEG C progress gradient freezings of temperature, phenanthrene is obtained by condensation dehydration
Ox leech freeze-dried powder c；Hiruto freeze-dried powder c is subjected to low-temperature grinding at 5 DEG C and crosses the hiruto after 50 meshes obtain object processing.
The gradient freezing dry gradient temperature stage is 12 temperature stages, specially -50 DEG C~-40 DEG C temperature stage 8h, -40 DEG C
~-35 DEG C of temperature stage 5h, -35 DEG C~-20 DEG C temperature stage 5h, -20 DEG C~-15 DEG C temperature stage 4h, -15 DEG C~-5 DEG C temperature
Spend stage 5h, -5 DEG C~0 DEG C temperature stage 5h, 0 DEG C~8 DEG C temperature stage 4h, 8 DEG C~15 DEG C temperature stage 6h, 15 DEG C~25
DEG C temperature stage 3h, 25 DEG C~30 DEG C temperature stage 5h, 30 DEG C~35 DEG C temperature stage 5h, 35 DEG C~45 DEG C temperature stage 8h.
Claims (10)
1. a kind of concocting method of hiruto, it is characterized in that: the concocting method of the hiruto the following steps are included:
1) hiruto is cleaned, cleans and dries, the water of hiruto weight percent 2~5% is then added, then in temperature
Freezing 3h~5h obtains material a to icing at -20 DEG C of degree；
2) material a is beaten with beater and is slurried to obtain material b；
3) material b is placed in freeze-drying disk, with the dry 40h~60h of -50 DEG C~45 DEG C progress gradient freezings of temperature, is passed through
Condensation dehydration obtains hiruto freeze-dried powder c；
4) hiruto freeze-dried powder c progress low-temperature grinding is sieved to obtain the hiruto after object processes.
2. the concocting method of hiruto according to claim 1, it is characterized in that: the cleaning is with flowing pure water pair
Hiruto carries out spray washing.
3. the concocting method of hiruto according to claim 1, it is characterized in that: the gradient freezing drying is from low temperature
Stage gradually arrives the drying process of hot stage.
4. the concocting method of hiruto according to claim 1 or 3, it is characterized in that: the ladder that the gradient freezing is dry
Degree temperature stage is 12 temperature stages.
5. the concocting method of hiruto according to claim 4, it is characterized in that: 12 temperature stages are -50 DEG C
~-40 DEG C, -40 DEG C~-35 DEG C, -35 DEG C~-20 DEG C, -20 DEG C~-15 DEG C, -15 DEG C~-5 DEG C, -5 DEG C~0 DEG C, 0 DEG C~8
DEG C, 8 DEG C~15 DEG C, 15 DEG C~25 DEG C, 25 DEG C~30 DEG C, 30 DEG C~35 DEG C, 35 DEG C~45 DEG C.
6. the concocting method of hiruto according to claim 5, it is characterized in that: described -50 DEG C~-40 DEG C temperature stages
Drying time be 5~10h.
7. the concocting method of hiruto according to claim 5, it is characterized in that: described -40 DEG C~-35 DEG C, -35 DEG C
~-20 DEG C, -20 DEG C~-15 DEG C, -15 DEG C~-5 DEG C, -5 DEG C~0 DEG C, 0 DEG C~8 DEG C, 8 DEG C~15 DEG C, 15 DEG C~25 DEG C, 25 DEG C
The drying time of~30 DEG C and 30 DEG C~35 DEG C of temperature stages is respectively 3~6h.
8. the concocting method of hiruto according to claim 5, it is characterized in that: 35 DEG C~-45 DEG C temperature stages
Drying time be 5~10h.
9. the concocting method of hiruto according to claim 1, it is characterized in that: the low-temperature grinding be temperature 2~
10 DEG C of crushing.
10. the concocting method of hiruto according to claim 1, it is characterized in that: the low-temperature grinding sieving is low temperature
It crushed 50 meshes.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006176408A (en) * | 2004-12-20 | 2006-07-06 | Shingy:Kk | Method for producing powder of dried hirudo, powder of dried hirudo obtained by the production method and thrombolysis promotion composition containing the powder of dried hirudo |
| CN103417580A (en) * | 2013-09-04 | 2013-12-04 | 南宁市净雪皇生物工程有限公司 | Method for preparing poecilobdella manillensis medium-high activity anticoagulant crude product |
| CN108653336A (en) * | 2018-07-30 | 2018-10-16 | 北京卫仁中药饮片厂 | A kind of concocting method of hiruto |
-
2019
- 2019-05-10 CN CN201910388308.0A patent/CN110075127A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006176408A (en) * | 2004-12-20 | 2006-07-06 | Shingy:Kk | Method for producing powder of dried hirudo, powder of dried hirudo obtained by the production method and thrombolysis promotion composition containing the powder of dried hirudo |
| CN103417580A (en) * | 2013-09-04 | 2013-12-04 | 南宁市净雪皇生物工程有限公司 | Method for preparing poecilobdella manillensis medium-high activity anticoagulant crude product |
| CN108653336A (en) * | 2018-07-30 | 2018-10-16 | 北京卫仁中药饮片厂 | A kind of concocting method of hiruto |
Non-Patent Citations (1)
| Title |
|---|
| 谭赫等: "水提法和仿生提取法研究菲牛蛭及其炮制品的抗血栓活性", 《中国药师》 * |
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