CN110068536A - A kind of optical system and implementation method of real time nucleic acid detection melting temperature - Google Patents
A kind of optical system and implementation method of real time nucleic acid detection melting temperature Download PDFInfo
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- CN110068536A CN110068536A CN201910180403.1A CN201910180403A CN110068536A CN 110068536 A CN110068536 A CN 110068536A CN 201910180403 A CN201910180403 A CN 201910180403A CN 110068536 A CN110068536 A CN 110068536A
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Abstract
The invention discloses the optical systems and implementation method of a kind of real time nucleic acid detection melting temperature, and wherein system includes: optical microscopy；The side of optical microscopy is provided with light source, and the other side is connected with fiber spectrometer by optical fiber, and fiber spectrometer is connect with computer；The substrate for solidifying sample to be tested is equipped with below the object lens of optical microscopy, substrate is fixed on a thermal station, is kept the temperature by thermal station to sample；The side of the thermal station is provided with the miniflow pump for basad upper injection matched samples；The light that the light source issues is radiated on sample to be tested and substrate via the object lens of optical microscopy, light forms interference in the light beam that the upper and lower surfaces reflection of substrate generates, interference light converges on the object lens of optical microscopy, after passing to fiber spectrometer conversion via optical fiber, transitional information is passed into computer, computer analyze to transitional information and output spectrum data, and the melting temperature of nucleic acid is obtained from spectroscopic data.
Description
Technical field
The present invention relates to reflection interference spectrum, temperature control, micro-fluidic and analysis fields, and in particular to a kind of real-time detection core
The optical system and implementation method of melting temperature.
Background technique
Optical correlation detection method has the advantages that speed is fast, high sensitivity, non-damaged data tiny sampler, makes it extensively
Applied in the detection of bio-molecular interaction.Wherein, the detection of DNA sequence dna and oligonucleotide fragment is widely used to cure
Learn diagnosis, biological study, many fields such as food industry monitoring and environmental monitoring.But not homotactic making nucleic acid molecular hybridization tool
There is different melting temperatures, so the Tm for accurately determining oligonucleotide hybridization will have more realistic meaning and practical value.
Currently, at home and abroad all favorably using up the report for learning to do phase method to be analyzed nucleic and melting temperature, Darby
Seminar has obtained the melting temperature of the DNA molecular of different serobilas using the method for fluorescent marker, and the disadvantages of this solution is desirable
DNA molecular is marked, experimental result is inaccurate, and can not real-time monitor DNA molecular melting temperature (Darby,
R.A.J.,et al.,High throughput measurement of duplex,triplex and quadruplex
melting curves using molecular beacons and a LightCycler.Nucleic Acids
Research,2002.30(9):p.e39)；Robert Miller seminar is passed through using surface plasma body resonant vibration to difference
The analysis of DNA probe melting temperature can be used for quickly detecting and identify to plant virus, and the disadvantages of this solution is due to the table
Surface plasma Optical devices are more sensitive to temperature, so testing result is inaccurate, anti-interference ability it is poor (Zhou, J.C.,
et al.,Immobilization-mediated reduction in melting temperatures of DNA–DNA
and DNA–RNA hybrids:Immobilized DNA probe hybridization studied by
SPR.Colloids&Surfaces A Physicochemical&Engineering Aspects,2015.481:p.72-
79)；In addition, Florian Prol seminar is filled using optics reflection interference spectrum system and that compound slab substrate combines
It sets to detect the melting temperature of DNA molecular, the disadvantages of this solution is that the stability of bio-sensing chip is poor, and testing result is inaccurate
(F.,et al.,Label-free characterisation of oligonucleotide hybridisation
using reflectometric interference spectroscopy.Analytical&Bioanalytical
Chemistry,2005.382(8):p.1889-1894).Above-mentioned optical means exists in the detection to nucleic and melting temperature
It needs to mark sample, poor anti jamming capability can not carry out nucleic and melting temperature accurate detection real-time, quickly.
Summary of the invention
For above-mentioned problems of the prior art, the object of the present invention is to provide a kind of real time nucleic acid detection unwinding temperature
The optical system and implementation method of degree.
In order to realize above-mentioned task, the invention adopts the following technical scheme:
A kind of optical system of real time nucleic acid detection melting temperature, comprising:
Optical microscopy；The side of optical microscopy is provided with light source, and the other side is connected with fiber spectrometer by optical fiber,
Fiber spectrometer is connect with computer；
The substrate for solidifying sample to be tested is equipped with below the object lens of optical microscopy, substrate is fixed on a thermal station
On, the temperature of sample to be tested is adjusted by thermal station；
The side of the thermal station is provided with the miniflow pump for basad upper injection matched samples；
The light that the light source issues is radiated on sample to be tested and substrate via the object lens of optical microscopy, and light is in base
The light beam that the upper and lower surfaces reflection at bottom generates forms Fabry-Perot interference, and interference light converges at optical microscopy
On object lens, after passing to fiber spectrometer conversion via optical fiber, transitional information is passed into computer, computer is to transitional information
Analyze simultaneously output spectrum data, the melting temperature of nucleic acid is obtained from spectroscopic data.
Further, the sample to be tested is single stranded DNA, and matched samples are the DNA molecular matched with single stranded DNA.
Further, the substrate is gold-plated porous nano alumina substrate, the upper and lower surface of substrate having a size of
2cmx2cm, the aperture in substrate are 50nm-80nm, and hole depth is 6-8 μm, plated thickness 10-30nm.
Further, the object lens enlargement ratio of the optical microscopy be 10~100 times, numerical aperture be 0.3~
0.7。
Further, the light source is halogen lamp, power 100W, wave band: 350~2500nm；Colour temperature: 2915K.
Further, the fiber spectrometer is refrigeration mode face battle array back-illuminated type spectrometer, for acquiring to test sample
Interference light signal is converted into intensity-wavelength spectral signal by the interference light signal of product；The wherein wave band 325 of fiber spectrometer
~1100nm, slit are less than or equal to 25 μm, and resolution ratio is less than or equal to 1.98nm, and signal-to-noise ratio is more than or equal to 1000:1.
Further, optical fiber wave band is 250~1100nm, and core diameter is 200 μm.
Further, the thermal station power 600W, workbench range 20cmx20cmx9cm, temperature-control range: room temperature~
200 DEG C, transformation rate: 0.01 DEG C/min.
Further, miniflow pump channel the number 1-4, flow rates 0.001-230ml/min.
A kind of implementation method of the optical system of real time nucleic acid detection melting temperature, comprising the following steps:
Step 1, prepare sample to be tested and with the matched samples that it is matched, then solidify sample to be tested in substrate, then
The substrate of cured sample to be tested is fixed on thermal station, thermal station is placed on the objective table of optical microscopy, is then adjusted
The temperature of whole thermal station opens miniflow pump, matched samples is injected in substrate with quantifying constant speed to required test temperature；
Step 2, light source is opened, the light that light source issues is radiated at sample to be tested, in substrate, and the interference light of substrate reflection is converged
Gather on the object lens of optical microscopy, passes to fiber spectrometer via optical fiber, interference light signal is converted by fiber spectrometer
Intensity-wavelength spectral signal, then passes to computer for spectral signal；
Step 3, it is constantly changing the temperature of substrate in fact by thermal station, repeats step 2, computer passes through under different temperatures
Intensity-wavelength spectral signal generate optical thickness-temperature continuous spectrum, be nucleic acid at the inflection point on continuous spectrum
Melting temperature.
Compared with prior art, the present invention having following technical characterstic:
1. this programme design has microfluidic system, quantitative control is carried out to micro-sampling, positioning dynamic detection can be carried out.
2. this programme design has analysis system, program processing is carried out to the reflection interference spectrum information that receives, it can be into
Row real-time detection.
3. this programme uses the good substrate of thermal stability, anti-interference ability is higher, and testing result can be made more accurate.
4. this programme devises temperature control system, real-time change can be carried out to base reservoir temperature, can continuously be detected.And
The device forms detection system, and temperature control system, analysis system integration, the operation is more convenient.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of present system；
(a), (b) of Fig. 2 is respectively surface topography, the sectional view of gold-plated porous nano alumina substrate；
(a), (b) of Fig. 3 is respectively intensity-wavelength light spectrogram, the optical thickness-temperature of gold-plated nanoporous anodic aluminum oxide
Spend spectrogram；
(a), (b) of Fig. 4 is respectively intensity-wavelength of gold-plated nanoporous anodic aluminum oxide Yu DNA molecular composite material
Spectrogram and optical thickness-temperature spectra figure；
In figure: 1 light source, 2 optical microscopies, 3 fiber spectrometers, 4 optical fiber, 5 thermal stations, 6 gold-plated porous nano aluminium oxide
Substrate, 7 miniflows pump, 8 computers.
Specific embodiment
Basic ideas of the invention are to be beaten on sample to be tested by the light that light source issues by optical microphotograph endoscope objective lens；
Sample to be tested reflection interference light converges in object lens, after the light of convergence passes to fiber spectrometer conversion via optical fiber, by optical fiber light
Spectrometer passes information to computer；Thermal station can be automatic accurate control sample temperature, fiber spectrometer is by different temperatures
Under interference of light information pass to computer, computer carries out interference of light information by the matched software program of spectrometer real-time
Effective spectroscopic data is analyzed and exported, the melting temperature of nucleic acid can be intuitively obtained.
As shown in Figure 1, the invention discloses a kind of optical systems of real time nucleic acid detection melting temperature, comprising:
Optical microscopy；The side of optical microscopy is provided with light source, and the other side is connected with fiber spectrometer by optical fiber,
Fiber spectrometer is connect with computer；Wherein:
The object lens enlargement ratio of optical microscopy is 10~100 times, and numerical aperture is 0.3~0.7.
Light source is halogen lamp, power 100W, wave band: 350~2500nm；Colour temperature: 2915K；It is used in the present embodiment
HL2000 halogen light source.
Fiber spectrometer is refrigeration mode face battle array back-illuminated type spectrometer, for acquiring the interference light signal from sample to be tested,
Interference light signal is converted into intensity-wavelength spectral signal；Wherein 325~1100nm of wave band of fiber spectrometer, slit are small
In being equal to 25 μm, resolution ratio is less than or equal to 1.98nm, and signal-to-noise ratio is more than or equal to 1000:1.
Optical fiber wave band is 250~1100nm, and core diameter is 200 μm.
The substrate for solidifying sample to be tested is equipped with below the object lens of optical microscopy, substrate is fixed on a thermal station
On, the temperature of sample to be tested is adjusted by thermal station；Wherein:
Substrate is gold-plated porous nano alumina substrate, and the upper and lower surface of substrate is porous in substrate having a size of 2cmx2cm
The aperture of structure is 50nm-80nm, and hole depth is 6-8 μm, plated thickness 10-30nm.
Thermal station power 600W, workbench range 20cmx20cmx9cm, temperature-control range: room temperature~200 DEG C, transformation rate:
0.01℃/min。
The side of the thermal station is provided with the miniflow pump for basad upper injection matched samples；The miniflow
Pump channel number 1-4, flow rates 0.001-230ml/min；Pipe used by miniflow pumps is PEEK pipe 1/16OD.
The light that light source issues is radiated on sample to be tested and substrate via the object lens of optical microscopy, and light is in substrate
Two light beams that upper and lower surfaces reflection generates generate interference effect, are formed Fabry-Perot interference (2nL=m λ),
Middle n is refractive index, and L is base film thickness, and the minor change (OTeff=nL) of effective optical thickness can by spectral information
It reflects.
Interference light converges on the object lens of optical microscopy, after passing to fiber spectrometer conversion via optical fiber, will convert
Information passes to computer, and computer analyze to transitional information and output spectrum data, and nucleic acid is obtained from spectroscopic data
Melting temperature.
Invention further provides a kind of implementation methods of the optical system of real time nucleic acid detection melting temperature, including with
Lower step:
Step 1, the matched samples for preparing sample to be tested and matching with it, in the present embodiment, the sample to be tested is
Single stranded DNA, matched samples are the DNA molecular matched with single stranded DNA；
Then solidify single stranded DNA in substrate, then the substrate of cured single stranded DNA is fixed on thermal station, by temperature control
Platform is placed on the objective table of optical microscopy, then adjusts the temperature of thermal station to required test temperature, opening miniflow pump will
DNA molecular is injected in substrate with quantifying constant speed, flow velocity 0.008-0.02ml/min；
Step 2, light source is opened, the light that light source issues is radiated at sample to be tested, in substrate, and the interference light of substrate reflection is converged
Gather on the object lens of optical microscopy, passes to fiber spectrometer via optical fiber, interference light signal is converted by fiber spectrometer
Intensity-wavelength spectral signal, then passes to computer for spectral signal；
Step 3, it is constantly changing the temperature of substrate in fact by thermal station, repeats step 2, computer passes through under different temperatures
Intensity-wavelength spectral signal generate optical thickness-temperature continuous spectrum, be nucleic acid at the inflection point on continuous spectrum
Melting temperature.
Embodiment:
In the present embodiment, we are using aperture 50nm, with a thickness of 8 μm of gold-plated porous nano alumina substrates as base
Bottom, shape appearance figure are as shown in Figure 2.As shown in figure 3, we can intuitively find out from figure, and variation of the substrate with temperature, light
It learns thickness not change, it was demonstrated that the thermal stability of the gold-plated porous nano alumina substrate is high, is more suitable for grinding
Study carefully the substrate of nucleic acid molecules melting temperature.
Miniflow pump injects pairing DNA molecular in substrate with certain flow rate, and light is beaten from micro objective and produced in substrate
Reflection interference light is given birth to, reflection interference light is passed information after converting via fiber spectrometer by micro objective and optical fiber
Pass computer；Thermal station can be passed the spectral information under different temperatures with real-time control sample temperature, fiber spectrometer simultaneously
Pass computer；Computer handles interference of light information in real time and exports effective spectroscopic data, i.e. optical thickness-temperature
Continuous spectrum.
Since dehybridization procedure has occurred during the alternating temperature in the DNA molecular in gold-plated porous nano alumina substrate, make
Refractive index in hole becomes larger, i.e., optical thickness changes, it is believed that being in the changed a certain temperature of optical thickness should
The melting temperature of DNA molecular, as shown in Figure 4；Reflect on the diagram, as at inflection point.By the Optical devices, we can be real-time
For accurate detection to the melting temperature of DNA molecular, the present embodiment is at 90 ° or so of inflection point.
This set proposed by the present invention can be with the optical system of real time nucleic acid detection melting temperature, so that analysis module is integrated
Change；In addition, gold-plated nano-porous alumina is utilized as the substrate for solidifying DNA, since the specific surface area of its material is high, heat is steady
It is qualitative more preferable, keep testing result more accurate.The substrate of the program is gold-plated nano-porous alumina material, in the prior art not
See using the material analysis large biological molecule.This programme uses the variation of reflection interference method analyzing molecules structure, in the prior art
It the use of reflection interference method analysis macromolecular structure is all generally to adhere to macromolecular in flat surface, and this programme is then by upper and lower
The reflection interference on surface analyzes signal, uses nano-pore as substrate.
Claims (10)
1. a kind of optical system of real time nucleic acid detection melting temperature characterized by comprising
The side of optical microscopy (2) is provided with light source (1), and the other side is connected with fiber spectrometer (3), optical fiber light by optical fiber
Spectrometer (3) is connect with computer (8)；
The substrate (6) for solidifying sample to be tested is equipped with below the object lens of optical microscopy (2), substrate (6) is fixed on a temperature
It controls on platform (5), the temperature of sample to be tested is adjusted by thermal station (5)；
The miniflow pump (7) that matched samples are injected on basad (6) is provided with positioned at the side of the thermal station (5)；
The light that the light source (1) issues is radiated on sample to be tested and substrate (5) via the object lens of optical microscopy (2), light
Line forms Fabry-Perot interference in the light beam that the upper and lower surfaces reflection of substrate (6) generates, and interference light converges at light
On the object lens for learning microscope (2), after passing to fiber spectrometer (3) conversion via optical fiber (4), transitional information is passed into calculating
Machine (8), computer (8) analyze to transitional information and output spectrum data, and the unwinding temperature of nucleic acid is obtained from spectroscopic data
Degree.
2. the optical system of real time nucleic acid detection melting temperature as described in claim 1, which is characterized in that described to test sample
Product are single stranded DNA, and matched samples are the DNA molecular matched with single stranded DNA.
3. the optical system of real time nucleic acid detection melting temperature as described in claim 1, which is characterized in that the substrate
It (6) is gold-plated porous nano alumina substrate, having a size of 2cmx2cm, the aperture in substrate (6) is for the upper and lower surface of substrate (6)
50nm-80nm, hole depth are 6-8 μm, plated thickness 10-30nm.
4. the optical system of real time nucleic acid detection melting temperature as described in claim 1, which is characterized in that the optics is aobvious
The object lens enlargement ratio of micro mirror (2) is 10~100 times, and numerical aperture is 0.3~0.7.
5. the optical system of real time nucleic acid detection melting temperature as described in claim 1, which is characterized in that the light source
It (1) is halogen lamp, power 100W, wave band: 350~2500nm；Colour temperature: 2915K.
6. the optical system of real time nucleic acid detection melting temperature as described in claim 1, which is characterized in that the optical fiber light
Spectrometer (3) is refrigeration mode face battle array back-illuminated type spectrometer 3, and for acquiring the interference light signal from sample to be tested, interference light is believed
Number it is converted into intensity-wavelength spectral signal；Wherein 325~1100nm of wave band of fiber spectrometer (3), slit are less than or equal to 25
μm, resolution ratio is less than or equal to 1.98nm, and signal-to-noise ratio is more than or equal to 1000:1.
7. the optical system of real time nucleic acid detection melting temperature as described in claim 1, which is characterized in that optical fiber (4) wave band
For 250~1100nm, core diameter is 200 μm.
8. the optical system of real time nucleic acid detection melting temperature as described in claim 1, which is characterized in that the thermal station
(5) power 600W, workbench range 20cmx20cmx9cm, temperature-control range: room temperature~200 DEG C, transformation rate: 0.01 DEG C/min.
9. the optical system of real time nucleic acid detection melting temperature as described in claim 1, which is characterized in that the miniflow pump
(7) port number 1-4, flow rates 0.001-230ml/min.
10. a kind of implementation method of the optical system of real time nucleic acid detection melting temperature, which comprises the following steps:
Step 1, then the matched samples for preparing sample to be tested and matching with it solidify sample to be tested on substrate (5), then will
The substrate (5) of cured sample to be tested is fixed on thermal station (6), and thermal station (6) is placed in the objective table of optical microscopy (2)
On, the temperature of thermal station (6) is then adjusted to required test temperature, opens miniflow pump (7), with quantifying constant speed by matched samples
It is injected on substrate (5)；
Step 2, light source (1) is opened, the light that light source (1) issues is radiated at sample to be tested, in substrate (5), substrate (5) reflection
Interference light converges on the object lens of optical microscopy (2), passes to fiber spectrometer (3) via optical fiber, and fiber spectrometer (3) will
Interference light signal is converted into intensity-wavelength spectral signal, and spectral signal is then passed to computer (8)；
Step 3, it is constantly changing the temperature of substrate (5) by thermal station (6), repeats step 2, computer (8) passes through not equality of temperature
Intensity-wavelength spectral signal under degree generates optical thickness-temperature continuous spectrum, is at the inflection point on continuous spectrum
The melting temperature of nucleic acid.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111426572A (en) * | 2020-03-13 | 2020-07-17 | 天津大学 | Constant-load stress corrosion on-line observation test device under hydrofluoric acid environment |
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| CN102016075A (en) * | 2008-05-06 | 2011-04-13 | 凯杰有限公司 | Simultaneous detection of multiple nucleic acid sequences in a reaction |
| CN102076870A (en) * | 2008-06-25 | 2011-05-25 | 实时基因组有限公司 | Method and apparatus for melting curve analysis of nucleic acids in microarray format |
| CN106053392A (en) * | 2016-05-19 | 2016-10-26 | 西北大学 | Device based on micro-nano fluid control reflection interference spectral imaging system and realization method |
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| CN102016075A (en) * | 2008-05-06 | 2011-04-13 | 凯杰有限公司 | Simultaneous detection of multiple nucleic acid sequences in a reaction |
| CN102076870A (en) * | 2008-06-25 | 2011-05-25 | 实时基因组有限公司 | Method and apparatus for melting curve analysis of nucleic acids in microarray format |
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| CN111426572A (en) * | 2020-03-13 | 2020-07-17 | 天津大学 | Constant-load stress corrosion on-line observation test device under hydrofluoric acid environment |
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