CN110029124B - Application of collard albino gene WKL1 in plant albino - Google Patents
Application of collard albino gene WKL1 in plant albino Download PDFInfo
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- CN110029124B CN110029124B CN201810208486.6A CN201810208486A CN110029124B CN 110029124 B CN110029124 B CN 110029124B CN 201810208486 A CN201810208486 A CN 201810208486A CN 110029124 B CN110029124 B CN 110029124B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
Abstract
The application belongs to the technical field of plant genetic engineering, and particularly relates to a key dominant gene which is specific to cruciferae and used for controlling low-temperature whitening of white kale W hite K ale L ocus 1（WKL1) Cloning, functional identification and use of (a). The nucleotide sequence of the gene is shown as SEQ ID NO. 1. The invention also relates toWKL1The promoter of the gene and the cloning of its recessive allele promoter. Through agrobacterium-mediated transformation of solanaceae tobacco and green cabbage, functional verification shows that the gene has an obvious function of controlling low-temperature albinism of white kale.KWL1The discovery of the gene provides a new gene resource for the cruciferous vegetables to generate new white ornamental varieties and simultaneously provides a new genetic resource for ornamental horticulture, and the utilization of the resource is beneficial to cultivating new ornamental plant varieties.
Description
Technical Field
This patent belongs to plant engineering technical field. In particular to the cloning, functional identification and application of a Cruciferae-specific key gene WKL1 for controlling the albation of white kale, and the gene can be used for preparing albino ornamental plants.
Background
The white kale is a favorite ornamental plant in autumn and winter, and has the function of flowers to a certain extent. White kale has many varieties, such as "white pigeon", "gull", "white crane", etc. The previous research shows that the newly grown leaf part of the white kale can only induce the generation of white color at low temperature (less than or equal to 15 ℃). Under the low temperature condition, no chloroplast structure is seen in the white region of the white kale, thereby generating a white phenotype. In 2014, a green common head cabbage genome is published, and convenience is provided for genetic positioning and cloning of target traits of cabbage vegetables (white common head cabbage and green common head cabbage are different cabbage vegetable varieties and belong to the same species).
The applicant finds that the white kale white character is an unreported white dominant character, the genetic rule of the white kale white dominant character is not analyzed, the gene for controlling the white color of the white kale is not cloned, and the function of the gene is not researched. Therefore, the functional study of the new gene WKL1 for controlling the white character of white kale is of great significance for analyzing the generation of the white character of white kale.
Disclosure of Invention
The invention aims to provide application of a kale albino gene WKL1 in plant albino. The white trait is incompletely dominant inheritance controlled by a single gene through genetic analysis, and is named as WKL1 (aWhiteKaleLocus 1). The CDS sequence of the gene is shown in SEQ ID NO.1, and the corresponding amino acid sequence is shown in SEQ ID NO. 2.
The invention also aims to provide a specific promoter of the kale albino gene WKL1, wherein the nucleotide sequence of the promoter is shown in SEQ ID NO. 3.
The last aim of the invention is to provide the application of the specific promoter of the kale albino gene WKL1 in plant albino, and the promoter can specifically start the expression of WKL1 so as to form albino characters.
In order to achieve the purpose, the invention adopts the following technical measures:
obtaining of kale albino gene WKL 1:
the invention is obtained by crossing natural mutant of red kale (the phenotype is a white kale type containing anthocyanin, and red and white are two independent genetic characters) and green common head cabbage haplotypeTo obtain F1Continuously backcrossing with the green common head cabbage haplotype to obtain BC1. Using BC1In the population, the BSR-seq and the traditional positioning method are combined, differential expression genes of the positioning sections are analyzed and gene function annotation is carried out, candidate genes are determined, and target genes for controlling white rare dominant natural mutation, namely WKL1 genes, are successfully cloned. The gene codes P450 protein, belongs to CYP708 protein family, and shows that the gene is a gene peculiar to the Cruciferae by evolution analysis, and other species do not have an ortholog of the gene, and do not have any report about the gene. The Cruciferae specific key gene WKL1 for controlling white kale whitening and its allele, the cDNA sequence of which is completely the same as shown in sequence table SEQ ID NO:1, including 1461 basic groups, the protein sequence table is shown in SEQ ID NO:2, encoding 486 amino acids. The invention comprises the acquisition of the promoter of the WKL1 gene and the promoter of the allele thereof, and the nucleotide sequences are respectively shown as SEQ ID NO.3 and SEQ ID NO. 4.
The application of the collard albino gene WKL1 in plant albino utilizes the conventional mode in the field to over-express the WKL1 gene (shown in SEQ ID NO: 1) in plants, and can obviously whiten the leaves of the plants. The gene can be used for genetic improvement in brassica cabbage crops and application in new variety cultivation.
In the above applications, preferably, the plant includes tobacco and green cabbage.
The application of the specific promoter of the collard albino gene WKL1 in plant albino can obtain albino cabbage plants by connecting the promoter with WKL1 and then complementarily expressing the promoter in green cabbages by using a conventional mode in the field.
The more detailed technical scheme is shown in the detailed description.
Compared with the prior art, the invention has the following advantages:
the invention firstly provides a albino gene of the kale, the gene is used as a very rare dominant gene for controlling white leaves, and the gene has important theoretical significance for understanding how the gene participates in inhibiting the formation of chloroplasts. In addition, the gene is not reported in researches of cruciferae, can provide a new gene resource for leaf color ornamental varieties of cruciferae, and is favorable for cultivating new ornamental varieties.
Drawings
FIG. 1: the over-expression phenotype of the WKL1 gene in the tobacco is complementary with the phenotype of the WKL1 gene in the cabbage.
Wherein: (a) is a tobacco transgene; (b) is a green cabbage complementation experiment.
Detailed Description
The technical schemes of the invention are conventional schemes in the field if not particularly stated; the reagents or materials, if not specifically mentioned, are commercially available.
Example 1:
kale albino gene WKL1(WhiteKaleLobtaining ocus 1):
1. genetic analysis of dominant mutant WKL1 phenotype
This study performed genetic analysis of mutant WKL1 phenotype. F is obtained by crossing natural mutant of red collard (white collard contains anthocyanin, red and white are two independent genetic characters) with green common head cabbage haplotype1Continuously backcrossing with the green common head cabbage haplotype to obtain BC1At its BC by genetic analysis1Medium white leaf: green leaves 304: 299 ≈ 1: 1 (P)>0.05), at BC1F2White leaves in the population: light white leaves: green leaf 27: 52: 20 ≈ 1: 2: 1 (P)>0.05). As a result, the white trait is incompletely dominant inheritance under single-gene control, and is named WKL1(WhiteKaleLocus 1). As a very rare dominant gene controlling white leaves, it is important to understand how it participates in the suppression of chloroplast formation, and it is of theoretical significance.
Map-based cloning of the WKL1 Gene
The WKL1 gene was initially mapped using a BSA coupled RNA-seq method, BSR-seq (Liu et al, 2012). Respectively selecting 50 white leaf plants and 50 green leaf plants, taking leaves with the same growth position and size, mixing and grinding, extracting total RNA, and performing RNA-seq sequencing. Will be provided withThe RNA-seq sequencing data of the two pools were aligned to the cabbage reference genome "02-12" (Liu et al, 2014), SNP analysis was performed on the two pools using SAMtools (Li et al, 2009), and the Δ (SNP-index) mean value (Takagi et al, 2013) was calculated and graphically displayed using perl script. The results showed that there was a peak at the head of chromosome 3 in brassica oleracea, indicating that WKL1 was initially located on chromosome C03. Further by molecular labeling, WKL1 was initially located between molecular markers BoY001(0.3Mb) and BoY003(1.23 Mb). Screening all 603 BC strains by adopting the two molecular markers1In the population, recombinant individuals were selected, new markers were developed, and the target gene was finely located between BoY010(0.75Mb) and BoY011(0.82Mb), at a distance of about 70 kb. There are 16 orfs (open Reading frames) in this region, with a significant difference in expression level of about 30-fold between the two pools and only one CYP708a4(Bol015404) gene. Thus, CYP708a4(Bol015404) is considered a candidate gene for WKL 1. The Sanger sequencing result shows that the gene mutant is completely consistent with a CDS sequence of green common head cabbage (SEQ ID NO.1), and the difference is presumed to be caused by a promoter due to the difference of expression amounts of two alleles. Amplifying 2.5kb promoters of two alleles and comparing and analyzing, finding that 12 SNPs exist between the two allele promoters and 10bp deletion exists in the mutant, supposing that stronger TATA repeat enhancer is generated after 10bp deletion in the mutant, so that the expression level of WKL1 is increased, and then a white leaf phenotype is generated, wherein the sequence of the promoter in the mutant is shown in SEQ ID NO. 3.
Example 2:
the application of the collard albino gene WKL1 in plant albino:
homologous recombination of CDS sequence (shown in SEQ ID NO.1) of WKL1 gene into Hellsgate2 vector, transformation of Agrobacterium, overexpression with 35S promoter, transformation of tobacco, and obtaining of 10 overexpression T1Passage positive shoots, 4 lines of which had white phenotypes (a in FIG. 1) and which are phenotypically consistent with the genotype. Homologous recombination of WKL1 gene self promoter and WKL1 gene full-length sequence (shown by SEQ ID NO.1 and SEQ ID NO. 3) into Hellsgate8 vector, and adoption of white kale WKL1 gene self promoter and white kale WKL1 geneThe full-length function complementation of the WKL1 gene is transferred into green cabbage to obtain three positive plants, and the phenotype is that the leaves become white or the yellowing is serious (b in figure 1). The experimental result proves that the WKL1 gene, namely CYP708A4 has the function of whitening the leaves.
Sequence listing
<110> university of agriculture in Huazhong
Application of <120> kale albino gene WKL1 in plant albino
<160>4
<170>SIPOSequenceListing 1.0
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<211>1461
<212>DNA
<213> Artificial Sequence (Artificial Sequence)
<400>1
atgcattgtt caaggcacaa caacatgagc ttgctttgga tcacaggatt gtgtgttaca 60
gccctagtgg ttgtgaggat tagcaattgg tggtaccgat ggtcaaaccc caagtctaat 120
ggcatgttac ctccgggatc aatgggcttc cccataatcg gagagacgtt cgattttttt 180
aagccgcatg gattctacga gatctccccg tttctcaaga agaagatgtt aaggtatgga 240
cctttgtttc ggactagcat tcttggtgta aacactgtaa tttctacaga tatggatgtg 300
aacttggata ttttacgaca cgagaacgag tattttaatt taagttatcc agatggttta 360
gtgaagccat tgggaaaaga aagcatgttc ttgaagacgg gaaacatcca caagcacatc 420
aaaaaaatct gtatgcgtct tttgggctcc gagaatttga agcggaagat aataaaagat 480
atggatcacg tgacatgcga gcatcttaat ttgaaggctg gccaaagaag agtcgacctc 540
agggacctag tttcaagttt gataacagcc cacttgacac caaaagtgat aagtgatctc 600
aaaccagaga ctcaatcaat tcttatggaa agcttcaaag ccttcagttt tgattggttt 660
aggacatcct atattctctc tgcgggaagg ggtctctaca aaaccctttg ggcatgcaga 720
caggggatga agttgataaa ggatgtttac acgagtagaa taacgtcaag agagaagtac 780
aatgacttcc ttgagacagc actagaagag ttagagaaag aaggaagctc agtgaacgaa 840
gatgtgattg taagcctcat cttcactctt tccggtatca ctcaagatac cacctctaag 900
gccatttgca tggttgtgaa attcatatcg gaaaacccaa aagttcttgt agagttgaag 960
agagagcatg aggcaatcct tgcgaacaga gaagataaag aaggcggagt tacttgggaa 1020
gaattcagac acaagatgac tttcaccaac atggttatac atgagtcgct tcgaatgaca 1080
aatttggccc ctatgatgtt tagaaaagtg gtgaaagatg tggaaataaa aggatatacg 1140
attccagcag attggatagt gttggttata ccttcactgg ttcattttga ccctgaaatt 1200
tatgataatc catttgagtt taatccgtgg agatgggagg ggaaggagtt gcggtctggg 1260
tctaaaacgt tcatggtgtt tggagcagga gccagacagt gcgctggttc agagtatgca 1320
aggcttcaga tttcactgtt cattcatcat cttgtcacca cttacgattt ctccttatcc 1380
aaagactgcg aggtgattcg ggtaccaggt gctctcttcc ccaatggcat ctctatgaac 1440
atctccaagc gttccaagtg a 1461
<210>2
<211>486
<212>PRT
<213> Artificial Sequence (Artificial Sequence)
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Met His Cys Ser Arg His Asn Asn Met Ser Leu Leu Trp Ile Thr Gly
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Leu Cys Val Thr Ala Leu Val Val Val Arg Ile Ser Asn Trp Trp Tyr
20 25 30
Arg Trp Ser Asn Pro Lys Ser Asn Gly Met Leu Pro Pro Gly Ser Met
35 40 45
Gly Phe Pro Ile Ile Gly Glu Thr Phe Asp Phe Phe Lys Pro His Gly
50 55 60
Phe Tyr Glu Ile Ser Pro Phe Leu Lys Lys Lys Met Leu Arg Tyr Gly
65 70 75 80
Pro Leu Phe Arg Thr Ser Ile Leu Gly Val Asn Thr Val Ile Ser Thr
85 90 95
Asp Met Asp Val Asn Leu Asp Ile Leu Arg His Glu Asn Glu Tyr Phe
100 105 110
Asn Leu Ser Tyr Pro Asp Gly Leu Val Lys Pro Leu Gly Lys Glu Ser
115 120 125
Met Phe Leu Lys Thr Gly Asn Ile His Lys His Ile Lys Lys Ile Cys
130 135 140
Met Arg Leu Leu Gly Ser Glu Asn Leu Lys Arg Lys Ile Ile Lys Asp
145 150 155 160
Met Asp His Val Thr Cys Glu His Leu Asn Leu Lys Ala Gly Gln Arg
165 170 175
Arg Val Asp Leu Arg Asp Leu Val Ser Ser Leu Ile Thr Ala His Leu
180 185 190
Thr Pro Lys Val Ile Ser Asp Leu Lys Pro Glu Thr Gln Ser Ile Leu
195 200 205
Met Glu Ser Phe Lys Ala Phe Ser Phe Asp Trp Phe Arg Thr Ser Tyr
210 215 220
Ile Leu Ser Ala Gly Arg Gly Leu Tyr Lys Thr Leu Trp Ala Cys Arg
225 230 235 240
Gln Gly Met Lys Leu Ile Lys Asp Val Tyr Thr Ser Arg Ile Thr Ser
245 250 255
Arg Glu Lys Tyr Asn Asp Phe Leu Glu Thr Ala Leu Glu Glu Leu Glu
260 265 270
Lys Glu Gly Ser Ser Val Asn Glu Asp Val Ile Val Ser Leu Ile Phe
275 280 285
Thr Leu Ser Gly Ile Thr Gln Asp Thr Thr Ser Lys Ala Ile Cys Met
290 295 300
Val Val Lys Phe Ile Ser Glu Asn Pro Lys Val Leu Val Glu Leu Lys
305 310 315 320
Arg Glu His Glu Ala Ile Leu Ala Asn Arg Glu Asp Lys Glu Gly Gly
325 330 335
Val Thr Trp Glu Glu Phe Arg His Lys Met Thr Phe Thr Asn Met Val
340 345 350
Ile His Glu Ser Leu Arg Met Thr Asn Leu Ala Pro Met Met Phe Arg
355 360 365
Lys Val Val Lys Asp Val Glu Ile Lys Gly Tyr Thr Ile Pro Ala Asp
370 375 380
Trp Ile Val Leu Val Ile Pro Ser Leu Val His Phe Asp Pro Glu Ile
385 390 395 400
Tyr Asp Asn Pro Phe Glu Phe Asn Pro Trp Arg Trp Glu Gly Lys Glu
405 410 415
Leu Arg Ser Gly Ser Lys Thr Phe Met Val Phe Gly Ala Gly Ala Arg
420425 430
Gln Cys Ala Gly Ser Glu Tyr Ala Arg Leu Gln Ile Ser Leu Phe Ile
435 440 445
His His Leu Val Thr Thr Tyr Asp Phe Ser Leu Ser Lys Asp Cys Glu
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Val Ile Arg Val Pro Gly Ala Leu Phe Pro Asn Gly Ile Ser Met Asn
465 470 475 480
Ile Ser Lys Arg Ser Lys
485
<210>3
<211>2500
<212>DNA
<213> Artificial Sequence (Artificial Sequence)
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ctcttttctg cgggagtatc taatatatta aaagagaagt acacctttaa attgccgctt 60
agttttgcaa gttatttaca cttccatgcc actaagatat taactaaacc tatatttaag 120
gatattttgt cttttacggt ttaattaatg catttgttaa attaatatat ttctaattaa 180
aaacattaca acattaacat tccacggctc cactatcata ttcaacaacc aatacctatt 240
attcctcaat tcttcaattt atatacacag cttactgaat caagcaaggt tccatttctc 300
acctcttagc ttatacaaat cgattttcta tcttttataa acaaacaaaa tctcaatata 360
catcaaatct acaatattta tacatttagt tttgtattaa ttcatacaaa caatacaaat 420
ccatgtatca tatatctata tctatatata tataatatta acaactataa aatttaatga 480
taatcagtat acacaaatcg atttttccaa aatcataatg taacttaagg atcagacagt 540
tttaaatttc aaaattaatg tataaaatct aaccaaaaaa taacacctac ttaattgcga 600
tattagatcc tattatattt ctatataaaa ttttataatt aatgaggaat atttattata 660
acaataagat ttggaaacta aaacacacaa aaacaataat tttcttattt gttactaaat 720
cacataatta gttaccacaa ttattcattt cccttgactt tcccctagac ttgcggatca 780
aacaacttaa aattttaaaa aatttaacgt ataaaatata tccataattg acactttctt 840
acggaattta gatcataaat atctactatc aaactatttc ctatattctc gctaaccaat 900
atcaccttta atacaaactt aagcttattt ataatttttt atcaattaca aaaaaattac 960
attttcattt tattttctta cagatttaaa tcataatttt tatacgttcc aaatttaata 1020
gaaatttaac aatcatttta ttttcttaca aatgttataa ctaatgtacc atttaaaaat 1080
agcatactaa ctcataattg tctattttcc atatttttca ttttaaaaat ttgttcgcta 1140
cagatttttg acatggatct aattatataa atgttggatt tatactcaat attggtaata 1200
cattttacgt taaaacacat aaaaaaagta ctcgatttta tacaactaca tttacaaata 1260
taacattatg tacaaacaaa aattaaaata taaaattaaa tacccttgcg gtcgcacggg 1320
ttaagatcta gtttttctta tttatgaagg ccatttgtaa ttatctgtgg gaacaagaag 1380
taggacctta aatttgtcat gtcagaacga taatattgtg aaatggagcg tatagagtcg 1440
tagagaggaa ggcgtttttt tcatacgggg tcgagatatt ttaagggttt gatttagtct 1500
tattgttaat tagttaatta caatcacaaa aatattatcc atctctttag atttttaaaa 1560
aatatatata ataaattaaa attaaaaatt tagatagtat aagtataaat ataaatatct 1620
ttgatatttt ctagttgtat tcactgatga cactcataat tcatatttat aagactttaa 1680
accaaaaaat ttgtagatct tttttcttaa tccctaatat gcatatagta catacatagg 1740
aatctaataa attcattttt aaaattttag tatcaatttt tgacaaaaga aaaagtattt 1800
ccattaaatt tataactttc atctaaataa aataaaataa ataaataaat ttgcatgtta 1860
ttatttatta tataaatcaa ggattttata tttcagtata agaatatatt tttaatattt 1920
tataattata taaaatgtga atatattatt aaataattca tgtatctatt tttcaactag 1980
taataattat cccgcaaatt cattcgtctt aactaatggg ttagtattat atgaatttat 2040
taacaacgat taaaaccgta actatccata tctgtaaaat tttacatctg cactcgcaac 2100
tgctgcattt aaaccaatta aatcatacaa agaatcatat taattctaca ggcacaaagt 2160
attttgattt atcttttata ttttctgaat tttagtgata ctaaaaatac attatcagaa 2220
caaagataac attatatttc atctttatca aacagaaatc aaaagttgga aatttaagaa 2280
tgaaatattg ctatttattg aaatgtgaaa ctctatttta tttctatgtt aaaagaacct 2340
aaataagtga ttaactatgt ctatgttaga ttttaaatat taccaattga tataactatg 2400
tctatgttag atttttaata ccttgtgaca ttcttctatc cacgtaagta aatttacttt 2460
tttcatttta ggattcatca tctatatata tgacccacca 2500
<210>4
<211>2500
<212>DNA
<213> Artificial Sequence (Artificial Sequence)
<400>4
cggtagtatc taatatatta aaagagaagt acacctttaa attaccgctt agttttgcaa 60
gttatttaca cttccatgcc actaagatat taactaaacc tatatttaag gatattttgt 120
cttttacggt ttaattaatg catttgttaa attaatatat ttctaattaa aaacattaca 180
acattaacat tccacggctc cactatcata ttcaacaacc aatacctatt attcctcaat 240
tcttcaatta atatacacag cttactgaat caagcaaggt tccatttctc acctcttagc 300
ttatacaaat cgattttcta tcttttataa acaaacaaaa tctcaatata catcaaatct 360
acaatattta tacatttagt tttgtattaa ttcatacaaa caatacaaat ccatgtatca 420
tatatctata tctatatcta tatctatata tataatatta acaactataa aatttaatga 480
taatcagtat acacaaatcg atttttccaa aatcataatg taacttaagg atcagacagt 540
tttaaatttc aaaattaatg tataaaatcg aaccaaaaaa taacacctac ttaattgcga 600
tattagatcc tattatattt ctatataaaa ttttataatt aatgaggaat atttactata 660
acaaaaagat ttggaaacta aaacacacaa aaacaataat tttcttattt gttactaaat 720
cacataatta gttaccacaa ttattcattt cccttgactt tcccctagac ttgcggatca 780
aacaacttaa aattttaaaa aatttaacgt ataaaatata tccataattg acactttctt 840
acgaaattta gatcataaat atctactatc aaactatttc ctatattctc gctaaccaat 900
atcaccttta atacaaactt aagcttattt ataatttttt atcaattaca aaaaaattac 960
attttcattt tattttctta cagatttaaa tcataatttt tatacgttcc aaatttaata 1020
gaaatttaac aatcatttta ttttcttaca aatgttataa ctaatgtacc atttaaaaat 1080
agcatactaa ctcataattg tctattttcc atatttttca ttttaaaaat ttgttcgcta 1140
cagatttttg acatggatct aattatataa atgttggatt tatactcaat attggtaata 1200
cattttacgt taaaacacat aaaaaaagta ctcgatttta tacaactaca tttacaaata 1260
taacattatg tacaaacaaa aattaaaata taaaattaaa tacccttgca gtcgcacggg 1320
ttaagatcta gtttttctta tttatgaagg ccatttgtaa ttatctgtgg gaacaagaag 1380
taggacctta aatttgtcat gtcagaacga taatattgtg aaatggagcg tatagagtcg 1440
tggagaggaa ggcgtttttt tcatacgggg tcgagatatt ttaagggttt gatttagtct 1500
tattgttaat tagttaatta caatcacaaa aatattatcc atctctttag atttttaaaa 1560
aatatatata ataaattaaa attaaaaatt tagatagtat aagtataaat ataaatatct 1620
ttgatatttt ctagttgtat tcactgatga cactcataat tcatatttat aagactttaa 1680
accaaaaaaa atgtagatct tttttcttaa tccctaatat gcatatagta catacatagg 1740
aatctaataa attcattttt aaaattttag tatcaatttt tgacaaaaga aaaagtattt 1800
ccattaaatt tataactttc atctaaataa aataaaataa ataaataaat ttgcatgtta 1860
ttatttatta tataaatcaa ggattttata tttcagtata agaatatatt tttaatattt 1920
tataattata taaaatgtga atatattatt aaataattca tgtatctatt tttcaactag 1980
taataattat cccgcaaatt cattcgtctt aactaatggg ttagtattat atgaatttat 2040
taacaacgat taaaaccgta actatccata tctgtaaaat tttacatctg cactcgcaac 2100
tgctgcattt aaaccaatta aatcatacaa agaatcatat taattctaca ggcacaaagt 2160
attttgattt atcttttata ttttctgaat tttagtgata ctaaaaatac attatcagaa 2220
caaagataac attatatttc atctttatca aacagaaatc aaaagttgga aatttaagaa 2280
tgaaatattg ctatttattg aaatgtgaaa ctctatttta tttctatgtt aaaagaacct 2340
aaataagtga ttaactatgt ctatgttaga ttttaaatat taccaattga tataactatg 2400
tctatgttaa atttttaata ccttgtgaca ttcttctatc cacgtaagta aatttacttt 2460
tttcatttta ggattcatca tctatatata tgacccacca 2500
Claims (3)
1. Enhancing the expression of the nucleotide sequence shown in SEQ ID NO.1 or the amino acid sequence shown in SEQ ID NO.2 in promoting the cabbage or tobacco albinism.
2. The nucleotide sequence of the specific promoter of the gene WKL1 whitened by kale is shown in SEQ ID NO. 3.
3. Use of a specific promoter according to claim 2 to overexpress the nucleotide sequence shown in SEQ ID No.1 in cabbage or tobacco albinism.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
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