CN110018301A - A kind of combined type device for immunochromatography - Google Patents
A kind of combined type device for immunochromatography Download PDFInfo
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- CN110018301A CN110018301A CN201810019757.3A CN201810019757A CN110018301A CN 110018301 A CN110018301 A CN 110018301A CN 201810019757 A CN201810019757 A CN 201810019757A CN 110018301 A CN110018301 A CN 110018301A
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
Abstract
The present invention provides a kind of combined type device for immunochromatography.Specifically, the present invention is integrated column affinity chromatography and immune flow measurement detection technique, is formed combined integral detection, is realized the quick detection for being isolated and purified and being detected on a platform.Described device carries out the switching of position by the rotatability for flowing through liquid outlet that column chromatographs part lower end, and liquid flows to waste liquid tank when sample-adding washing, and when dissociation flows to chromatography strip.Sample enters horizontal lateral chromatography detection part, migrates under capillary action, complete detection process after vertical column chromatography device processing.Device provided by the invention is easy to operate, and sample needed for detecting is few, and detection time is short, can be not only used for qualitative detection, it can also be used to quantitative detection.
Description
Technical field
The present invention relates to field of biotechnology more particularly to a kind of combined type device for immunochromatography.
Background technique
In detection process, pre-treatment, including heating must be carried out for the sample of some complicated components, are centrifuged, cracking is pure
Change either organic extraction etc., sample meets the needs of detection so that treated.For example, 3 (AFP- of alpha-fetoprotein variant
L3), glycosylated hemoglobin (HbA1C) needs to be detected again after purification.
Alpha-fetoprotein (AFP) is divided into three classes: AFP-L1, AFP-L2 according to the affinity of itself and LcA (LCA)
And AFP-L3.Wherein, AFP-L1 cannot be in conjunction with LCA, and AFP-L3 can be in conjunction with LCA.AFP-L3, which is that one of HCC is new, to swell
Tumor markers have important value to the clinical diagnosis of HCC.It is separated by the binding ability difference to LCA, is then used
Immunological method carries out quantitative detection AFP-L3.It is numerous there are still operating although the detection technique of AFP-L3 is constantly being improved
The trivial, disadvantages such as detection time is too long, sensitivity is low.
HbA1C, i.e. glycosylated hemoglobin are a kind of forms of hemoglobin, before reflection a period of time (4-8 weeks)
Average plasma glucose concentration, frequently as screening, diagnosis and the effective means for assessing diabetes.Glycosyl is usually measured with chromatography
It is more to change hemoglobin, other there are also colorimetric method, electrophoresis and immunization etc..There are the various blood red eggs of glycosylation on the market at present
The detection kit of white (HbA1C).For example, the immunoturbidimetry of the companies such as Roche, Beijing Li Deman detects, Abbott Laboratories, Shanghai section
The enzyme process of Hua Deng company detects, the high performance liquid chromatography of the companies such as Bole, beauty Ai Lier, and above method or detection kit are real
Test that resultant error is big, cumbersome, sensitivity is low or cost is excessively high.
For the defect for solving the prior art, those skilled in the art are dedicated to developing a kind of included pre-treatment component, sample
Enter the device for immunochromatography of detection part after processing, and the device is easy to operate, at low cost, detection time is short.
Summary of the invention
The object of the present invention is to provide the novel detection devices of a kind of column chromatography for separation and immunochromatography detection combination, are used for
The target detection thing that separation and detection need pretreated detection sample and easily interfere with each other.
The first aspect of the present invention, provides a kind of combined type device for immunochromatography, and described device includes:
At least one lateral chromatography detector bar；
One shell, the shell is used to accommodate the lateral chromatography detector bar, and is equipped with for observing the lateral layer
Analyse the display window of the testing result of detector bar；
Column chromatographs part, and the column chromatography part is used to carry out chromatography processing to analysis sample；
Wherein, the column that the shell is additionally provided with for accommodating the column chromatography part chromatographs part contained structure；
The column chromatography part contained structure is equipped at least one sample export, so that flowing through the column chromatography part
The sample that at least part is handled through chromatography is flowed out from the sample export, and is added into that described at least one is lateral
Chromatograph the sample application zone of detector bar.
In another preferred example, the described column chromatography part is equipped with superposed chromatography media receiving portion, and under being located at
Side is used to collect the liquid confluence portion for flowing through liquid.
In another preferred example, column chromatography part is equipped with the first adding mouth for adding sample to be tested, and/or
For flowing through liquid outlet for the sample outflow through Image processing.
In another preferred example, column chromatography part contained structure is equipped with the entrance for being packed into the column chromatography part.
In another preferred example, column chromatography part contained structure is additionally provided at least one waste liquid outlet, so that
At least part waste liquid for flowing through the column chromatography part, flows out from the waste liquid outlet.
In another preferred example, the shell is additionally provided with liquid waste containment structure, and the liquid waste containment structure is for holding
Waste liquid described in receiving.
In another preferred example, the liquid waste containment structure is slot structure.
In another preferred example, the described column chromatography part be arranged to can to chromatograph relative to the column part contained structure into
Row rotation, so that the column chromatography part is in the first relative position and the second relative position, wherein when the column layer
When analysis part is in the first relative position, the liquid flowed out from column chromatography part is flowed out from the waste liquid outlet；When the column
When chromatography part is in the second relative position, the liquid flowed out from column chromatography part is flowed out from the sample export.
In another preferred example, the liquid confluence portion is equipped with a liquid outlet.
In another preferred example, when the column chromatography part is in the first relative position, the liquid in liquid confluence portion
Body outlet is corresponding with the position of the waste liquid outlet；When the column chromatography part is in the second relative position, liquid converges
The liquid outlet in stream portion is corresponding with the position of the sample export.
In another preferred example, described " rotation " especially the column chromatography part can the relatively described column chromatography part receiving knot
Structure is mobile and/or mobile relative to the lateral chromatography detector bar.
In another preferred example, the shell is horizontally disposed, and the column chromatography part contained structure is arranged to
Perpendicular to or substantially perpendicular to the shell.
In another preferred example, the shell and column chromatography part contained structure are dismountable mutually.
In another preferred example, the shell and column chromatography part contained structure are integrated.
In another preferred example, column chromatography part contained structure is located at least one lateral chromatography detector bar
It is loaded end.
In another preferred example, column chromatography part and the column chromatography part contained structure are dismountable mutually.
In another preferred example, the lateral chromatography detector bar is fixed in the shell.
In another preferred example, the column chromatography part is preinstalled with chromatographic stuffing.
In another preferred example, adsorption chromatography filler, Partition Chromatography filler, ion exchange are preinstalled in the column chromatography part
Chromatographic stuffing, gel permeation chromatography filler or affinity chromatography filler etc..
In another preferred example, affinity chromatography filler is preinstalled in the column chromatography part.
In another preferred example, the lateral chromatography detector bar include sequentially connected blotting paper, chromatographic film, reagent pad and
Sample pad.
In another preferred example, the lateral chromatography detector bar is also connected with whole blood seperation film.
In another preferred example, the blotting paper, chromatographic film, reagent pad and sample pad are attached in PVC board.
In another preferred example, it is fixed with T line area and C line area in the chromatographic film, is respectively used to detection and Quality Control.
In another preferred example, labelled antibody or labelled antigen containing marker are fixed on the reagent pad.
In another preferred example, the marker is selected from the group: colloidal gold, nano magnetic particle, colloidal-carbon, quantum dot,
Color micro-sphere, fluorescent microsphere, or combinations thereof.
In another preferred example, the sample pad is to use the processed polyester film of buffer solution or glass fibre in advance.
In another preferred example, described device includes at least the first lateral chromatography detector bar and the second lateral chromatography detects
Item is additionally provided with the second lateral chromatography detector bar sample holes of addition sample, the second lateral chromatography detector bar on the shell
It is wholly or substantially identical as the first lateral chromatography detector bar structure.
In another preferred example, the first lateral chromatography detector bar is for detecting 3 (AFP- of alpha-fetoprotein variant
L3)；The second lateral chromatography detector bar is for detecting alpha-fetoprotein (AFP).
In another preferred example, the first lateral chromatography detector bar is for detecting glycosylated hemoglobin (HbA1c)；
The second lateral chromatography detector bar is for detecting hemoglobin (Hb).
In another preferred example, the shell includes two layers completely cut off up and down, and upper layer is examined equipped at least one lateral chromatography
Item is surveyed, lower layer is equipped with liquid waste containment structure.
In another preferred example, sample includes blood sample, urine sample, milk, tissue fluid, saliva, sweat etc..
In another preferred example, the shell main structure body of described device is in key shaped, and as a result observation area is strip, can be matched
It closes instrument or reading apparatus carries out quantitative detection.
The second aspect of the present invention, provides the purposes of device as described in the first aspect of the invention, and described device is used for
The target detection thing that separation and detection need pretreated test sample or easily interfere with each other.
It should be understood that above-mentioned each technical characteristic of the invention and having in below (eg embodiment) within the scope of the present invention
It can be combined with each other between each technical characteristic of body description, to form a new or preferred technical solution.As space is limited, exist
This no longer tires out one by one states.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to do simply to introduce, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of invention for those of ordinary skill in the art without creative efforts, can be with
It obtains other drawings based on these drawings.
Fig. 1 shows combined type device for immunochromatography appearance lateral view of the invention.
Fig. 2 shows combined type device for immunochromatography appearance top view of the invention.
Fig. 3 shows combined type device for immunochromatography column chromatography part rotation birds-eye perspective of the invention.
Fig. 4 shows box body of the present invention (shell) upper layer perspective view.
Fig. 5 shows lateral immunochromatography detector bar structure chart of the invention.
Fig. 6 shows combined type device for immunochromatography sectional structure chart of the invention.
In each attached drawing, each mark is as follows:
Wherein, 1- shell；2- column chromatographs part；11- column chromatographs part contained structure；12- display window；13- waste liquid tank； 14-
Second lateral chromatography detector bar sample holes；21- chromatography media receiving portion；22- liquid confluence portion；23- flows through liquid outlet；31-
One lateral chromatography detector bar；32- the second lateral chromatography detector bar；111- waste liquid outlet；112- sample export；The first side 113- to
Chromatograph detector bar sample holes；311- blotting paper；312- chromatographic film；313- reagent pad；314- sample pad.
Specific embodiment
The present inventor is by extensive and in-depth research, by largely screening, unexpectedly obtains a kind of immune layer of combined type
Analysis apparatus is integrated two kinds of operation post affinity chromatographys and immune flow measurement detection technique, is realized and is carried out on a platform
The quick detection of separation and detection.On the one hand, position is carried out by the rotatability for flowing through liquid outlet that column chromatographs part lower end
Switching, liquid flows to waste liquid tank when sample-adding washing, and when dissociation flows to chromatography strip.On the other hand, one is increased in shell lower layer
A waste liquid tank is mutually common to storage waste liquid with column chromatography part, solves the problems, such as the whereabouts of waste liquid.Device provided by the invention is not required to
Will pass through additional sample process, required sample size to be detected is few, structure is simple, easy to operate, detection time is short, pollution compared with
Less, at low cost.
The present invention provides a kind of lateral chromatography detection device with preprocessing function, by column chromatography method and immunochromatography plate
Combine, forms combined integral detection, be provided simultaneously with the function of isolating and purifying and detect.Sample passes through vertical column layer
After analysis apparatus processing, horizontal lateral chromatography detection part is entered, is migrated under capillary action, completes detection process.Meanwhile
The key shape structure of the present apparatus allows to that reading apparatus is cooperated to use, and completes the function of quantitative detection.Device provided by the invention
Easy to operate, sample needed for detecting is few, and detection time is short, can be not only used for qualitative detection, it can also be used to quantitative detection.
Term
As used herein, term " preferred ", " optimal ", " typical ", " illustratively " or " optionally " does not limit this
The range of invention or embodiment.
As used herein, term "available" expression include or do not include and/or with or without the use of and/or realize or do not realize
And/or the selection or influence for occurring or not occurring, and the selection constitutes some embodiments of the present invention or its consequence at least
A part, without limiting the scope of the invention.
When enumerating a series of values, merely to it is convenient or succinct, including all possible money range and the range side
Single number in or around boundary.Any numerical value, unless otherwise defined, further including practical approximation, and integer value is not arranged
Except fractional value.Subrange value and practical approximation are not construed as special disclosed value.
Unless the context indicates otherwise, plural form is not excluded for referring to the object of singular.
Column chromatography
Column chromatography is separating mixture, goes deimpurity common method.Principle utilizes ingredient each in mixture
The difference of physical property, when selecting some condition that each ingredient is made to flow through supporting agent or adsorbent, each ingredient can be due to it
The difference of physical property and separated.Column chromatography is then the alternate progress of solid-liquid by adsoptivity and solubility according to both
A kind of chromatographic technique of distribution.It is chromatographed by column, the purity of the target detection thing in sample and concentration can be made to greatly improve,
From the influence of impurity in detection, to improve the accuracy of detection.
The affinity chromatography separation covalently bound affinity column of HbA1c:m- amino phenyl boric acid agarose can be used for microtrabeculae point
Analysis detection.After hemoglobin in blood sample is added to chromatographic column, all glycosylated hemoglobins (HbA1 and side chain saccharification
Hemoglobin, total glycosylated hemoglobin) in conjunction with boric acid rather than glycosylated hemoglobin is by chromatographic column to be measured.It is high being added
Concentration also includes the polyhydroxy base complex of cis-hydroxyl groups, such as after sorbierite, the combination of glycosylated hemoglobin and boric acid is replaced
And it is eluted from pillar.Influence phase of the affinity chromatography to the translated hemoglobin modified and pathology hemoglobin later
To insensitive.Using affinity chromatography, it is only capable of measuring total glycosylated hemoglobin.Widely used affinity chromatography method, allows to use
Empirical algorithms calculate " HbA1c of standard " from total saccharification Hemoglobin Value.
Affinity column chromatography method separates AFP-L3: with the AFP- in LcA (LCA) affinity chromatography post separation serum
L3 is common AFP heteroplasmon isolation and purification method.Column is inserted after LCA and cnbr-activated sepharose 4b coupling
It is interior, LCA affinity column is made；After serum sample is added to chromatographic column, make AFP-L3 in conjunction with LCA, is not associated with part through layer
It is flowed out after analysis column；AFP-L3 is eluted from LCA by eluent again, to achieve the purpose that isolate and purify AFP-L3.
Lateral immunochromatography
The principle of lateral immunochromatography is that special antibody or antigen are first fixed on to a certain zone of reaction film, and sample exists
Capillarity drive moves down when moving to the region for being fixed with antibody or antigen, and corresponding test substance is i.e. and the antibody in sample
Or antigen is specifically bound.Typical lateral immunochromatography detection is divided into sandwich and two kinds of competition law.Sandwich type
Lateral flow detection commonly used in can provide at least two epitopes macromolecular determinand detection.Competitive assay is then used
In the detection for not having the small molecule determinand there are two independent antibody combining site.One lateral flow immunochromatography device is logical
It often include a sample pad, a conjugate release pad, a reaction film and an absorption pad.Certain applications also need specific complete
Blood seperation film.
Main advantages of the present invention
(1) switching of position, liquid when sample-adding washs are carried out by the rotatability for flowing through liquid outlet that column chromatographs part lower end
Body flows to waste liquid tank, and when dissociation flows to chromatography strip.Therefore, device provided by the invention needs not move through additional sample process,
Sample needed for detecting is few, and structure is simple, easy to operate, to save time, detection time short.
(2) waste liquid tank is increased in shell lower layer and be mutually common to storage waste liquid with column chromatography part, solve waste liquid
Whereabouts problem, it is easy to operate, at low cost and reduce pollution.
(3) the key shape structure of the present apparatus allows to that reading apparatus is cooperated to use, and completes the function of quantitative detection.The present invention
The device of offer can be not only used for qualitative detection, it can also be used to quantitative detection.
Present invention will be further explained below with reference to specific examples.It should be understood that these embodiments are merely to illustrate the present invention
Rather than it limits the scope of the invention.In addition, attached drawing is schematic diagram, therefore apparatus of the present invention and equipment are not shown by described
The size or ratio of intention limit.
Embodiment 1
As shown in figures 1 to 6, present embodiments provide a kind of combined type device for immunochromatography include: shell 1, column chromatography part 2,
First lateral chromatography detector bar 31 and the second lateral chromatography detector bar 32.
First lateral chromatography detector bar 31 and 32 structure of the second lateral chromatography detector bar are wholly or substantially identical.Described first
Lateral chromatography detector bar 31 and the second lateral chromatography detector bar 32 include sequentially connected blotting paper 311, chromatographic film 312, reagent
Pad 313 and sample pad 314.The blotting paper 311, chromatographic film 312, reagent pad 313 and sample pad 314 are attached in PVC board.
The chromatographic film 312 can select different brands, aperture according to detectable substance and climb speed, predominantly Millipore, Sartorius,
The NC film of the brands such as Pall.Be fixed in chromatographic film 312 in conjunction with antigen or antibody in T line area and C line area, be respectively used to detection and
Quality Control.Labelled antibody or labelled antigen containing marker are fixed on the reagent pad 313.The sample pad 314 is to use in advance
The processed polyester film of buffer solution or glass fibre.The lateral chromatography detector bar is also connected with whole blood seperation film, invests
On sample pad.
Column chromatographs part 2 and is used to carry out chromatography processing to analysis sample, is installed in column chromatography part contained structure 11.
The described column chromatography part 2 includes superposed chromatography media receiving portion 21 and underlying flows through liquid for collecting
Liquid confluence portion 22, chromatography media receiving portion 21 are equipped with the first adding mouth for adding sample to be tested, liquid confluence portion 22
Bottom, which is equipped with, flows through liquid outlet 23.In one embodiment, chromatography media receiving portion 21 and liquid confluence portion 22 are integral type structure
It makes.In one embodiment, the column chromatography part 2 is chromatographic column.Different chromatographic stuffings can be pre-installed in chromatographic column for not
With the separation demand of sample.
Shell 1 includes the box body of the first lateral chromatography detector bar 31 and the second lateral chromatography detector bar 32, and for holding
The column for carrying column chromatography part 2 chromatographs part contained structure 11.The column chromatography part contained structure 11 is located at one end of box body.Implement one
In example, box body can be cuboid, and contained structure is tubular, interior to be equipped with cavity, and cavity chromatographs part contained structure 11 for placing column.
Box body is horizontally disposed, and the column chromatography part contained structure 11 is arranged to perpendicular to or substantially perpendicular to the box body.
In one embodiment, the box body and column chromatography part contained structure 11 are dismountable mutually.In another preferred example, institute
The box body and the column chromatography part contained structure 11 stated are integrated.It can be layered by partition in box body, wherein on
Layer is waste liquid layer, also known as waste liquid tank 13 for placing lateral chromatography detector bar 31 and 32, lower layer, can be used for collecting waste liquid.
The column chromatography 11 lower end of part contained structure is equipped with waste liquid outlet 111 and sample export 112.Sample export 112 with
First lateral chromatography detector bar sample holes 113 are same component, and the sample export 112 is located at first lateral chromatography
The top of the sample pad 314 (i.e. sample application zone) of detector bar 31.The a part for flowing through the column chromatography part 2 is handled through chromatography
Sample, flowed out from the sample export 112, and the sample pad 314 for being added into the first lateral chromatography detector bar 31 (is loaded
Area).
Box body is equipped with display window 12 and the second lateral chromatography detector bar sample holes 14.The display window 12 is used for
The testing result of lateral chromatography detector bar is observed, the second lateral chromatography detector bar sample holes 14 are used to sample being added to second side
To chromatography detector bar 32.Waste liquid tank 13 is located at the lower section of waste liquid outlet 111, is connected with waste liquid outlet 111, for collect from
The waste liquid that waste liquid outlet 111 flows out.
In one embodiment, column chromatography part 2 is arranged to that part contained structure can be chromatographed relative to the column
11 are rotated, so that the column chromatography part 2 is in the first relative position and the second relative position, wherein when described
When column chromatography part 2 is in the first relative position (Fig. 2), the liquid flowed out from column chromatography part 2 is flowed from the waste liquid outlet 111
Out；When the described column chromatography part be in the second relative position (Fig. 3), from the liquid of column chromatography part outflow from the sample
112 outflow of outlet.
In another preferred example, when the column chromatography part 2 is in the first relative position (Fig. 2), the liquid confluence
The liquid outlet 23 that flows through of 22 lower end of portion with the position of the waste liquid outlet 111 is corresponding；When the described column chromatography part is in the
The liquid outlet 23 that flows through of Fig. 3 when two relative positions, 22 lower end of liquid confluence portion with the position of the sample export 112 are corresponding
's.
During loading, column chromatography 2 lower end of part flow through liquid outlet 23 and waste liquid outlet 111 communicate, waste liquid is through waste liquid
Outlet 111 flows into waste liquid tank 13.After the completion of sample-adding, column chromatography part 2 is rotated by 90 °, and the liquid outlet 23 that flows through of lower end turns therewith
It is dynamic, it is communicated with sample export 112 (sample holes 113 of the first lateral chromatography detector bar).Eluent is added at this time, through isolating and purifying
Sample through the first lateral chromatography detector bar sample holes 113 enter the first lateral chromatography detector bar 31, detect target detection thing.Separately
Outside, the sample not isolated and purified can be entered into the second lateral chromatography detector bar 32 through the second lateral chromatography detector bar sample holes 14
Starting detection.By comparing the intensity of the T line (detection line) of two detector bars, it can calculate the percentage of target detection thing
Than.
In another preferred example, the main structure body of the shell is in key shaped, and as a result observation area is strip, and it is fixed to cooperate
It measures detector or reading apparatus carries out quantitative detection.
Embodiment 2
In the combined type device for immunochromatography of embodiment 1, LCA and cnbr-activated sepharose 4b are coupled
It inserts in column afterwards, LCA affinity column is made.At this point, column chromatography part 2 flow through liquid outlet 23 and waste liquid outlet 111 communicate.It will
After serum sample is added to column chromatography part 2, make AFP-L3 in conjunction with LCA, is not associated with part after column chromatographs part 2 through waste liquid outlet
111 flow into waste liquid tank 13.Column chromatography part 2 is rotated by 90 °, and make its lower end flows through liquid outlet 23 and the detection of the first lateral chromatography
Sample holes 113 communicate.Eluent is added, AFP-L3 is eluted to from LCA and entered the first lateral chromatography detector bar 31, is used for
Detect the AFP-L3 after isolating and purifying.Furthermore it is possible to by the sample not isolated and purified through the second lateral chromatography detector bar sample holes
14 are added to the second lateral chromatography detector bar 32, for detecting the total amount of AFP in sample.Compare the first side by display window 12
To the intensity of chromatography detector bar 31 and the T line (detection line) of the second lateral chromatography detector bar 32, it can calculate AFP-L3's
Percentage.
Embodiment 3
In the combined type device for immunochromatography of embodiment 1, it is m- amino phenyl boric acid agarose covalent bond that column, which chromatographs part 2,
Affinity column.At this point, column chromatography part 2 flow through liquid outlet 23 and waste liquid outlet 111 communicate.Hemoglobin in blood sample is added
To column chromatograph part 2 after, all glycosylated hemoglobins (HbA1c and side chain saccharification hemoglobin, total glycosylated hemoglobin) with
Boric acid combines, and both the non-glycated hemoglobin part flows into waste liquid tank 13 after column chromatographs part 2 through waste liquid outlet 111.Column chromatographs part 2
It is rotated by 90 °, communicates the liquid outlet 23 that flows through of its lower end with the first lateral chromatography detector bar sample holes 113.High concentration is added
Also after polyhydroxy base complex such as sorbierite comprising cis-hydroxyl groups, the combination of HbA1c and boric acid is replaced and elutes from pillar
Get off, and enter the first lateral chromatography detector bar 31, for detecting the HbA1c after isolating and purifying.In addition, will can not isolate and purify
Sample be added to the second lateral chromatography detector bar 32 through the second lateral chromatography detector bar sample holes 14, for detecting blood in sample
The total amount of Lactoferrin.Compare the T of the first lateral chromatography detector bar 31 and the second lateral chromatography detector bar 32 by display window 12
The intensity of line (detection line), it can calculate the percentage of HbA1c.
All references mentioned in the present invention is incorporated herein by reference, independent just as each document
It is incorporated as with reference to such.In addition, it should also be understood that, after reading the above teachings of the present invention, those skilled in the art can
To make various changes or modifications to the present invention, such equivalent forms equally fall within model defined by the application the appended claims
It encloses.
Claims (10)
1. a kind of combined type device for immunochromatography, which is characterized in that described device includes:
At least one lateral chromatography detector bar；
One shell, the shell are used to accommodate the lateral chromatography detector bar, and are equipped with for observing the lateral chromatography inspection
Survey the display window of the testing result of item；
Column chromatographs part, and the column chromatography part is used to carry out chromatography processing to analysis sample；
Wherein, the column that the shell is additionally provided with for accommodating the column chromatography part chromatographs part contained structure；
The column chromatography part contained structure is equipped at least one sample export, so that flowing through the column chromatography part at least
The sample that a part is handled through chromatography is flowed out from the sample export, and is added at least one described lateral chromatography
The sample application zone of detector bar.
2. device as described in claim 1, which is characterized in that it is useless that the column chromatography part contained structure is additionally provided at least one
It is flowed out so that flowing through at least part waste liquid of the column chromatography part from the waste liquid outlet liquid outlet.
3. device as claimed in claim 2, which is characterized in that the column chromatography part is arranged to can be relative to the column
Chromatography part contained structure is rotated, so that the column chromatography part is in the first relative position and the second relative position,
Wherein when the described column chromatography part be in the first relative position, from the liquid of column chromatography part outflow from the waste liquid outlet
Outflow；When the column chromatography part is in the second relative position, the liquid flowed out from column chromatography part goes out from the sample
Mouth outflow.
4. device as described in claim 1, which is characterized in that the shell is horizontally disposed, and the column chromatographs part
Contained structure is arranged to perpendicular to or substantially perpendicular to the shell.
5. device as described in claim 1, which is characterized in that the column chromatography part and the column chromatograph part contained structure
It is dismountable mutually.
6. device as described in claim 1, which is characterized in that the column chromatography part is preinstalled with chromatographic stuffing.
7. device as described in claim 1, which is characterized in that the lateral chromatography detector bar includes sequentially connected water suction
Paper, chromatographic film, reagent pad and sample pad.
8. device as described in claim 1, which is characterized in that described device includes at least the first lateral chromatography detector bar and the
Two lateral chromatography detector bars, are additionally provided with the second lateral chromatography detector bar sample holes of addition sample on the shell, and described second
Lateral chromatography detector bar and the first lateral chromatography detector bar structure are wholly or substantially identical.
9. device as described in claim 1, which is characterized in that the shell includes two layers completely cut off up and down, and upper layer is equipped with extremely
A few lateral chromatography detector bar, lower layer are equipped with liquid waste containment structure.
10. the purposes of combined type device for immunochromatography as described in claim 1, which is characterized in that described device is for separating
The target detection thing for needing pretreated test sample with detection or easily interfering with each other.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810019757.3A CN110018301A (en) | 2018-01-09 | 2018-01-09 | A kind of combined type device for immunochromatography |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810019757.3A CN110018301A (en) | 2018-01-09 | 2018-01-09 | A kind of combined type device for immunochromatography |
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| CN110018301A true CN110018301A (en) | 2019-07-16 |
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|---|---|---|---|
| CN201810019757.3A Pending CN110018301A (en) | 2018-01-09 | 2018-01-09 | A kind of combined type device for immunochromatography |
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