CN110006970A - Preparation method of electrochemical sensor for dopamine detection and products thereof and application - Google Patents
Preparation method of electrochemical sensor for dopamine detection and products thereof and application Download PDFInfo
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- CN110006970A CN110006970A CN201910389700.7A CN201910389700A CN110006970A CN 110006970 A CN110006970 A CN 110006970A CN 201910389700 A CN201910389700 A CN 201910389700A CN 110006970 A CN110006970 A CN 110006970A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/308—Electrodes, e.g. test electrodes; Half-cells at least partially made of carbon
Abstract
The present invention provides a kind of preparation method of electrochemical sensor and products thereof and application for dopamine detection, and the sensor includes: electrode matrix, decorative layer and nanoparticle, wherein the electrode matrix is the electrode matrix of glass carbon materials matter；The chemical modification layer is bromophenol blue layer；Nanoparticle is IrO2- Pt, the electrochemical sensor can be realized the instant detection to dopamine.The sensor is to develop the novel electrochemical sensor for being used to detect dopamine in biological sample based on nanotechnology, electrochemical analysis techniques and sensor technology of preparing is combined.A kind of quick, sensitive, easy electrochemical sensor for dopamine detection is provided.
Description
Technical field
The present invention relates to preparation methods of a kind of electrochemical sensor and products thereof and application, especially a kind of to be used for DOPA
Preparation method of electrochemical sensor of amine detection and products thereof and application.
Background technique
Parkinson's disease is that one kind often betides the elderly's central nervous system degenerative disease, and clinical symptoms are mainly shown as
Static tremor, bradykinesia, hypermyotonia etc., disease incidence is improved with the increase at age, if patient does not obtain
Timely reasonable Clinics and Practices, it is easy to cause physical function to decline, or even can't take care of oneself, finally occur various concurrent
Disease, such as the infection of pneumonia, uropoietic system.Progression of the disease to advanced stage will whole body it is stiff, be unable to leave the bed, lead to organ failure
And threat to life.
Studies have shown that dopamine is as very important neurotransmitter a kind of in organism, its concentration and Parkinson's disease
Generation close relation.It can be seen that the level of detection dopamine is of great significance to the research of Parkinson's disease pathology.
Dopamine detection method mainly includes fluorescence method, chromatography, ultraviolet spectrophotometry, polarography, gas-chromatography-matter
Compose combination method etc. and capillary electrophoresis etc..But these detection method detection cycles are long, program is complicated, required reagent is various,
Be no longer satisfied modern measure needs.Therefore find a kind of sensitive, the efficient method for detecting dopamine is particularly important.
Electrochemical measuring technique is due to having many advantages, such as that high sensitivity, speed are fast, easy to operate and be widely used in biological sample
The detection of product.Since the content of dopamine in organism is very low, the electrochemistry for developing high sensitivity measuring dopamine is passed
Sensor is of great significance.
Summary of the invention
Long, program complexity the deficiency for detection cycle existing for existing dopamine test method, present invention aims at
A kind of preparation method of electrochemical sensor for dopamine detection is provided.
Another object of the present invention is: providing a kind of electrochemical sensing for dopamine detection of above method preparation
Device product.
Another object of the present invention is to: a kind of application of the said goods is provided.
The object of the invention is realized by following proposal:
A kind of preparation method of the electrochemical sensor for dopamine detection, the electrochemical sensor includes: electrode base
Body, decorative layer and nanoparticle, wherein the electrode matrix is the glass-carbon electrode of glass carbon materials matter；The chemical modification layer
For bromophenol blue layer；Nanoparticle is IrO2- Pt, comprising the following steps:
After glass-carbon electrode is processed by shot blasting, it is cleaned by ultrasonic in secondary distilled water；
Glass-carbon electrode after cleaning is put into the phosphate buffer solution of pH=5.0~7.0, in -0.2V~+2.0V current potential model
In enclosing, scan round is carried out with the sweep speed of 15-20mV/s and handled for 10~20 periods；
Glass-carbon electrode after scan round is put into the phosphate buffer solution of the bromophenol blue containing 0.1~1.0mmol/L, and the buffering is molten
PH=5.0~7.0 of liquid are followed in -1.0V~+1.8V potential range with the sweep speed of 15~20mV/s again
6~30 periods of ring scan process；
It after being cleaned with secondary distilled water, will be put into containing a certain concentration after scan round treated glass-carbon electrode takes out
IrCl3, K2PtCl6And Na2SO4Solution in, in certain potential range, with the sweep speed of 5~20mV/s again into
Row scan round handles 6~40 periods；
It will be cleaned after scan round treated glass-carbon electrode takes out with secondary distilled water, the electrochemistry for being made described passes
Sensor.
Wherein, the IrCl3、 K2PtCl6And Na2SO4Concentration is 0.1~1.0mmol/L.
The present invention provides a kind of electrochemical sensors for dopamine detection, are prepared according to the above method.
The present invention also provides the applications that a kind of electrochemical sensor is used for dopamine detection.
Electrochemical sensor of the present invention is to be based on sensor technology of preparing mutually tying nanotechnology, electrochemical analysis techniques
It closes, develops the novel electrochemical sensor for being used to detect dopamine in biological sample.It provides a kind of quick, sensitive, simple
Just the electrochemical sensor product of dopamine detection.The electrochemical sensor can be realized the instant detection to dopamine.
Detailed description of the invention
Fig. 1 is sample detection figure associated with electrode prepared by embodiment 4 and electrochemical workstation.
Specific embodiment
Embodiment is implemented under the premise of the technical scheme of the present invention, gives detailed embodiment and specific
Operating process, but protection scope of the present invention is not limited to following embodiments.
Embodiment 1
A kind of preparation method of the electrochemical sensor for dopamine detection, which is characterized in that the electrochemical sensor
It include: electrode matrix, decorative layer and nanoparticle, wherein the electrode matrix is the glass-carbon electrode of glass carbon materials matter；Described
Chemical modification layer is bromophenol blue layer；Nanoparticle is IrO2- Pt, according to the following steps:
After glass-carbon electrode is processed by shot blasting, it is cleaned by ultrasonic in secondary distilled water；
Glass-carbon electrode after cleaning is put into the phosphate buffer solution of pH=5.0~7.0, in -0.2V~+2.0V current potential model
In enclosing, scan round is carried out with the sweep speed of 20mV/s and handles 10 periods；
Glass-carbon electrode after scan round is put into the phosphate buffer solution of the bromophenol blue containing 0.4mmol/L, the buffer solution
PH=7.0 are carried out scan round again with the sweep speed of 20mV/s and handled for 6 week in 0.0V~+1.0V potential range
Phase；
After glass-carbon electrode after secondary cycle scan process is taken out, after being cleaned with secondary distilled water, it is put into containing 0.1
The IrCl of mmol/L3, the K of 0.2mmol/L2PtCl6With the Na of 0.4 mmol/L2SO4Solution in, in -0.4V~+1.0V
In potential range, carries out scan round again with the sweep speed of 20mV/s and handle 6 periods；
It will be cleaned after scan round treated glass-carbon electrode takes out with secondary distilled water, the electrochemistry for being made described passes
Sensor.
This sensor and electrochemical workstation are combined, to 1.0 × 10-10 mol/L ~1.0×10-4 Mol/L concentration
Sample is detected respectively.
Embodiment 2
A kind of preparation method of the electrochemical sensor for dopamine detection, according to the following steps:
After glass-carbon electrode is processed by shot blasting, it is cleaned by ultrasonic in secondary distilled water；
Glass-carbon electrode after cleaning is put into the phosphate buffer solution of pH=5.0, in -0.2V~+1.0V potential range,
Scan round, which is carried out, with the sweep speed of 15mV/s handled for 13 periods；
Glass-carbon electrode after scan round is put into the phosphate buffer solution of the bromophenol blue containing 0.1mmol/L, the buffer solution
PH=5.0 are carried out scan round again with the sweep speed of 15mV/s and handle 10 in 0.0V~+1.0V potential range
Period；
After glass-carbon electrode after secondary cycle scan process is taken out, the phosphorus of pH=5.0 is put into after being cleaned with secondary distilled water
In hydrochlorate buffer solution, in -0.2V~+1.0V potential range, with the sweep speed of 15 mV/s, scan round processing is carried out
13 periods；
After scan round treated glass-carbon electrode takes out, after being cleaned with secondary distilled water, it is put into containing 1 mmol/L's
IrCl3, the K of 0.8mmol/L2PtCl6With the Na of 0.6mmol/L2SO4Solution in, in -0.2V~+1.0V potential range
It is interior, it carries out scan round again with the sweep speed of 15mV/s and handles 10 periods；
It will be cleaned after scan round treated glass-carbon electrode takes out with secondary distilled water, the electrochemistry for being made described passes
Sensor.This sensor and electrochemical workstation are combined, to 1.0 × 10-10 mol/L ~1.0×10-4 The sample of mol/L concentration
It is detected respectively.
Embodiment 3
A kind of preparation method of the electrochemical sensor for dopamine detection, according to the following steps:
After glass-carbon electrode is processed by shot blasting, it is cleaned by ultrasonic in secondary distilled water；
Glass-carbon electrode after cleaning is put into the phosphate buffer solution of pH=7.0, in -0.2V~+2.0V potential range,
Scan round, which is carried out, with the sweep speed of 20mV/s handles 20 periods；
Glass-carbon electrode after scan round is put into the phosphate buffer solution of the bromophenol blue containing 1.0mmol/L, the buffer solution
PH=7.0 are carried out scan round again with the sweep speed of 20mV/s and handle 30 in 0.0V~+1.0V potential range
Period；
After glass-carbon electrode after secondary cycle scan process is taken out, after being cleaned with secondary distilled water, it is put into pH=7.0
In phosphate buffer solution, in -0.2V~+2.0V potential range, with the sweep speed of 20 mV/s, scan round is carried out
Handle 20 periods；
It is put into the IrCl containing 0.2 mmol/L3, the K of 0.1mmol/L2PtCl6With the Na of 0.3 mmol/L2SO4Solution in,
In -0.3V~+1.0V potential range, carries out scan round again with the sweep speed of 10mV/s and handle 30 periods；
It will be cleaned after scan round treated glass-carbon electrode takes out with secondary distilled water, the electrochemistry for being made described passes
Sensor.This sensor and electrochemical workstation are combined, to 1.0 × 10-10 mol/L ~1.0×10-4 The sample of mol/L concentration
It is detected respectively.
Embodiment 4
A kind of preparation method of the electrochemical sensor for dopamine detection, according to the following steps:
After glass-carbon electrode is processed by shot blasting, it is cleaned by ultrasonic in secondary distilled water；
Glass-carbon electrode after cleaning is put into the phosphate buffer solution of pH=7.0, in -0.2V~+2.0V potential range,
Scan round, which is carried out, with the sweep speed of 20mV/s handles 20 periods；
Glass-carbon electrode after scan round is put into the phosphate buffer solution of the bromophenol blue containing 0.1mmol/L, the buffer solution
PH=7.0 are carried out scan round again with the sweep speed of 20mV/s and handle 30 in 0.0V~+1.0V potential range
Period；
After glass-carbon electrode after secondary cycle scan process is taken out, after being cleaned with secondary distilled water, it is put into pH=5.0
In phosphate buffer solution, in -0.2V~+1.0V potential range, with the sweep speed of 15 mV/s, carry out at scan round
Manage 13 periods；
It after being cleaned with secondary distilled water, will be put into containing a certain concentration after scan round treated glass-carbon electrode takes out
0.2 mmol/L IrCl3, the K of 0.1mmol/L2PtCl6With the Na of 0.2 mol/L2SO4Solution in, -0.1V~+
In the potential range of 1.0V, carries out scan round again with the sweep speed of 15mV/s and handle 10 periods；
It will be cleaned after scan round treated glass-carbon electrode takes out with secondary distilled water, the electrochemistry for being made described passes
Sensor.This sensor and electrochemical workstation are combined, to without dopamine and 1.0 × 10-10 mol/L ~1.0×10-4
The sample of mol/L concentration is detected respectively, and sample detection figure is as shown in Figure 1, the electrochemical sensor shows dopamine
Good sensitivity.
Claims (4)
1. a kind of preparation method of the electrochemical sensor for dopamine detection, which is characterized in that the electrochemical sensing
Device includes: electrode matrix, decorative layer and nanoparticle, wherein the electrode matrix is the glass-carbon electrode of glass carbon materials matter；It is described
Chemical modification layer be bromophenol blue layer；Nanoparticle is IrO2- Pt, comprising the following steps:
After glass-carbon electrode is processed by shot blasting, it is cleaned by ultrasonic in secondary distilled water；
Glass-carbon electrode after cleaning is put into the phosphate buffer solution of pH=5.0~7.0, in -0.2V~+2.0V current potential model
In enclosing, scan round is carried out with the sweep speed of 15-20mV/s and handled for 10~20 periods；
Glass-carbon electrode after scan round is put into the phosphate buffer solution of the bromophenol blue containing 0.1~1.0mmol/L, and the buffering is molten
PH=5.0~7.0 of liquid are followed in -1.0V~+1.8V potential range with the sweep speed of 15~20mV/s again
6~30 periods of ring scan process；
It after being cleaned with secondary distilled water, will be put into containing a certain concentration after scan round treated glass-carbon electrode takes out
IrCl3, K2PtCl6And Na2SO4Solution in, in certain potential range, with the sweep speed of 5~20mV/s again into
Row scan round handles 6~40 periods；
It will be cleaned after scan round treated glass-carbon electrode takes out with secondary distilled water, the electrochemistry for being made described passes
Sensor.
2. the preparation method for the electrochemical sensor of dopamine detection according to claim 1, which is characterized in that described
IrCl3、 K2PtCl6And Na2SO4Concentration is 0.1~1.0mmol/L.
3. a kind of electrochemical sensor for dopamine detection, it is characterised in that method preparation according to claim 1 or claim 2
It obtains.
4. the application that a kind of electrochemical sensor according to claim 3 is used for dopamine detection.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111521659A (en) * | 2020-06-12 | 2020-08-11 | 上海纳米技术及应用国家工程研究中心有限公司 | Preparation method of electrochemical sensor for rapid detection of dopamine, product and application thereof |
| EP4008808A1 (en) | 2020-12-02 | 2022-06-08 | University of Wroclaw | Method of obtaining a sensor with a subnanomolar detection limit for determination of dopamine levels, especially in urine |
Citations (4)
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|---|---|---|---|---|
| EP0471330A1 (en) * | 1990-08-15 | 1992-02-19 | Degussa Ag | PH-measuring device |
| CN103031246A (en) * | 2011-10-10 | 2013-04-10 | 中国科学院电子学研究所 | Microelectrode array chip for multi-parameter detection of nerve cells and preparation method thereof |
| CN104076072A (en) * | 2014-06-25 | 2014-10-01 | 复旦大学 | High-sensitivity photoelectrochemical sensor made from iridium oxide-ferriporphyrin-titanium oxide and preparation method for sensor |
| CN105259224A (en) * | 2015-10-16 | 2016-01-20 | 上海纳米技术及应用国家工程研究中心有限公司 | Preparation method of electrochemical sensor for quick detection of dopamine |
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2019
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0471330A1 (en) * | 1990-08-15 | 1992-02-19 | Degussa Ag | PH-measuring device |
| CN103031246A (en) * | 2011-10-10 | 2013-04-10 | 中国科学院电子学研究所 | Microelectrode array chip for multi-parameter detection of nerve cells and preparation method thereof |
| CN104076072A (en) * | 2014-06-25 | 2014-10-01 | 复旦大学 | High-sensitivity photoelectrochemical sensor made from iridium oxide-ferriporphyrin-titanium oxide and preparation method for sensor |
| CN105259224A (en) * | 2015-10-16 | 2016-01-20 | 上海纳米技术及应用国家工程研究中心有限公司 | Preparation method of electrochemical sensor for quick detection of dopamine |
Non-Patent Citations (2)
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| YEJIN HA等: "Alteration of the morphology and electrocatalytic activity of IrO2 nanowires upon reduction by hydrogen gas", 《SENSORS AND ACTUATORS B: CHEMICAL》 * |
| 陈伟 等: "聚溴酚蓝修饰电极对多巴胺的电催化作用及伏安测定", 《分析试验室》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111521659A (en) * | 2020-06-12 | 2020-08-11 | 上海纳米技术及应用国家工程研究中心有限公司 | Preparation method of electrochemical sensor for rapid detection of dopamine, product and application thereof |
| EP4008808A1 (en) | 2020-12-02 | 2022-06-08 | University of Wroclaw | Method of obtaining a sensor with a subnanomolar detection limit for determination of dopamine levels, especially in urine |
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