CN110006970B - Preparation method of electrochemical sensor for dopamine detection, product and application thereof - Google Patents
Preparation method of electrochemical sensor for dopamine detection, product and application thereof Download PDFInfo
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- CN110006970B CN110006970B CN201910389700.7A CN201910389700A CN110006970B CN 110006970 B CN110006970 B CN 110006970B CN 201910389700 A CN201910389700 A CN 201910389700A CN 110006970 B CN110006970 B CN 110006970B
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Abstract
The invention provides a preparation method of an electrochemical sensor for detecting dopamine, a product and application thereof, wherein the sensor comprises the following components: the electrode comprises an electrode substrate, a modification layer and nano particles, wherein the electrode substrate is made of glassy carbon; the chemical modification layer is a bromophenol blue layer; the nano particle is IrO2-Pt, which enables the immediate detection of dopamine. The sensor is based on the combination of a nanotechnology, an electrochemical analysis technology and a sensor preparation technology, and a novel electrochemical sensor for detecting dopamine in a biological sample is developed. Provides an electrochemical sensor for quickly, sensitively and simply detecting dopamine.
Description
Technical Field
The invention relates to a preparation method of an electrochemical sensor, a product and application thereof, in particular to a preparation method of an electrochemical sensor for dopamine detection, a product and application thereof.
Background
Parkinson's disease is a degenerative disease of the central nervous system, which often occurs in the elderly, and the clinical symptoms of Parkinson's disease are mainly manifested as resting tremor, bradykinesia, increased muscular tension and the like, the incidence rate of Parkinson's disease is increased along with the increase of age, if patients are not diagnosed and treated reasonably in time, the physical function is easy to decline, even the life cannot be managed by themselves, and finally various complications such as pneumonia, mystery urinary system infection and the like occur. The disease will be stiff and bedridden in the late stage, resulting in organ failure and life threatening.
Research has shown that dopamine is a very important neurotransmitter in the organism, and the concentration of the dopamine is closely related to the occurrence of Parkinson's disease. Therefore, the detection of the dopamine level has important significance for the research of the pathology of the Parkinson disease.
The dopamine detection method mainly comprises a fluorescence method, a chromatography method, an ultraviolet spectrophotometry method, a polarography method, a gas chromatography-mass spectrometry combined method and the like, and a capillary electrophoresis method and the like. However, the detection methods have long detection period, complicated procedures and various required reagents, and can not meet the requirements of modern detection. Therefore, it is important to find a sensitive and efficient method for detecting dopamine.
The electrochemical detection technology is widely used for detecting biological samples due to the advantages of high sensitivity, high speed, simple operation and the like. Because the content of dopamine in organisms is very low, the development of an electrochemical sensor for measuring dopamine with high sensitivity is of great significance.
Disclosure of Invention
Aiming at the defects of long detection period and complex procedure of the existing dopamine detection method, the invention aims to provide a preparation method of an electrochemical sensor for dopamine detection.
Yet another object of the present invention is to: provides an electrochemical sensor product for dopamine detection prepared by the method.
Yet another object of the present invention is to: provides an application of the product.
The purpose of the invention is realized by the following scheme:
a method for preparing an electrochemical sensor for dopamine detection, said electrochemical sensor comprising: the electrode comprises an electrode substrate, a modification layer and nano particles, wherein the electrode substrate is a glassy carbon electrode made of glassy carbon; the chemical modification layer is a bromophenol blue layer; the nano particle is IrO2-Pt, comprising the steps of:
polishing the glassy carbon electrode, and then ultrasonically cleaning in secondary distilled water;
putting the cleaned glassy carbon electrode into phosphate buffer solution with pH = 5.0-7.0, and performing cyclic scanning treatment at a scanning rate of 15-20 mV/s within a potential range of-0.2V to + 2.0V for 10-20 periods;
placing the glassy carbon electrode subjected to cyclic scanning into a phosphate buffer solution containing 0.1-1.0 mmol/L bromophenol blue, wherein the pH of the buffer solution is = 5.0-7.0, and carrying out cyclic scanning again at a scanning speed of 15-20 mV/s within a potential range of-1.0V to + 1.8V for 6-30 periods;
taking out the glassy carbon electrode after the cyclic scanning treatment, cleaning the glassy carbon electrode with secondary distilled water, and putting IrCl with a certain concentration3，K2PtCl6And Na2SO4In the solution, cyclic scanning is carried out again for 6-40 periods at a scanning speed of 5-20 mV/s within a certain potential range;
and taking out the glassy carbon electrode subjected to the cyclic scanning treatment, and cleaning the glassy carbon electrode with secondary distilled water to obtain the electrochemical sensor.
Wherein, said IrCl3、 K2PtCl6And Na2SO4The concentration is 0.1 to 1.0 mmol/L.
The invention provides an electrochemical sensor for detecting dopamine, which is prepared according to the method.
The invention also provides an application of the electrochemical sensor in dopamine detection.
The electrochemical sensor is based on the combination of a nanotechnology, an electrochemical analysis technology and a sensor preparation technology, and develops a novel electrochemical sensor for detecting dopamine in a biological sample. Provides an electrochemical sensor product for quickly, sensitively, simply and conveniently detecting dopamine. The electrochemical sensor can realize the instant detection of dopamine.
Drawings
FIG. 1 is a diagram of the detection of a sample in which the electrode prepared in example 4 is used in combination with an electrochemical workstation.
DETAILED DESCRIPTION OF EMBODIMENT (S) OF INVENTION
The embodiments are implemented on the premise of the technical scheme of the invention, and detailed implementation modes and specific operation processes are given, but the protection scope of the invention is not limited by the following embodiments.
Example 1
The preparation method of the electrochemical sensor for detecting dopamine is characterized in that the electrochemical sensorThe sensor includes: the electrode comprises an electrode substrate, a modification layer and nano particles, wherein the electrode substrate is a glassy carbon electrode made of glassy carbon; the chemical modification layer is a bromophenol blue layer; the nano particle is IrO2-Pt, according to the following steps:
polishing the glassy carbon electrode, and then ultrasonically cleaning in secondary distilled water;
putting the cleaned glassy carbon electrode into phosphate buffer solution with pH = 5.0-7.0, and carrying out cyclic scanning treatment for 10 periods at a scanning rate of 20mV/s within a potential range of-0.2V to + 2.0V;
putting the glassy carbon electrode subjected to cyclic scanning into a phosphate buffer solution containing 0.4mmol/L bromophenol blue, wherein the pH of the buffer solution =7.0, and carrying out cyclic scanning again at a scanning speed of 20mV/s within a potential range of 0.0V to + 1.0V for 6 periods;
taking out the glassy carbon electrode after the secondary cycle scanning treatment, washing the glassy carbon electrode by using secondary distilled water, and adding IrCl containing 0.1mmol/L30.2mmol/L of K2PtCl6And 0.4mmol/L Na2SO4In the solution (a), cyclic scanning treatment is carried out again for 6 periods at a scanning rate of 20mV/s within a potential range of-0.4V to + 1.0V;
and taking out the glassy carbon electrode subjected to the cyclic scanning treatment, and cleaning the glassy carbon electrode with secondary distilled water to obtain the electrochemical sensor.
The sensor is used in combination with an electrochemical workstation for 1.0 × 10-10 mol/L ~1.0×10-4 The samples with mol/L concentration are respectively detected.
Example 2
A preparation method of an electrochemical sensor for dopamine detection comprises the following steps:
polishing the glassy carbon electrode, and then ultrasonically cleaning in secondary distilled water;
putting the cleaned glassy carbon electrode into phosphate buffer solution with pH =5.0, and performing cyclic scanning treatment at a scanning rate of 15mV/s within a potential range of-0.2V to + 1.0V for 13 periods;
putting the glassy carbon electrode subjected to cyclic scanning into a phosphate buffer solution containing 0.1mmol/L bromophenol blue, wherein the pH of the buffer solution =5.0, and performing cyclic scanning again at a scanning speed of 15mV/s within a potential range of 0.0V to + 1.0V for 10 periods;
taking out the glassy carbon electrode subjected to the secondary circulating scanning treatment, washing the glassy carbon electrode by using secondary distilled water, and then putting the glassy carbon electrode into a phosphate buffer solution with the pH =5.0, wherein the potential range is from-0.2V to + 1.0V, and the circulating scanning treatment is carried out for 13 periods at the scanning rate of 15 mV/s;
taking out the glassy carbon electrode after the cyclic scanning treatment, washing the glassy carbon electrode by using secondary distilled water, and then adding IrCl containing 1mmol/L30.8mmol/L of K2PtCl6And 0.6mmol/L Na2SO4In the solution (a), the cyclic scanning treatment is carried out again for 10 cycles at a scanning rate of 15mV/s in a potential range of-0.2V to + 1.0V;
and taking out the glassy carbon electrode subjected to the cyclic scanning treatment, and cleaning the glassy carbon electrode with secondary distilled water to obtain the electrochemical sensor. The sensor is used in combination with an electrochemical workstation for 1.0 × 10-10 mol/L ~1.0×10-4 The samples with mol/L concentration are respectively detected.
Example 3
A preparation method of an electrochemical sensor for dopamine detection comprises the following steps:
polishing the glassy carbon electrode, and then ultrasonically cleaning in secondary distilled water;
the cleaned glassy carbon electrode is put into phosphate buffer solution with pH =7.0, and cyclic scanning treatment is carried out for 20 periods at a scanning rate of 20mV/s within a potential range of-0.2V to + 2.0V;
placing the glassy carbon electrode subjected to cyclic scanning into a phosphate buffer solution containing 1.0mmol/L bromophenol blue, wherein the pH of the buffer solution =7.0, and performing cyclic scanning again at a scanning speed of 20mV/s within a potential range of 0.0V to + 1.0V for 30 periods;
taking out the glassy carbon electrode subjected to the secondary circulating scanning treatment, washing the glassy carbon electrode by using secondary distilled water, putting the glassy carbon electrode into phosphate buffer solution with the pH =7.0, and performing circulating scanning treatment for 20 periods at a scanning rate of 20mV/s within a potential range of-0.2V to + 2.0V;
IrCl with a concentration of 0.2mmol/L was added30.1mmol/L of K2PtCl6And 0.3 mmol/L Na2SO4In the solution (a), cyclic scanning treatment is carried out again for 30 cycles at a scanning rate of 10mV/s in a potential range of-0.3V to + 1.0V;
and taking out the glassy carbon electrode subjected to the cyclic scanning treatment, and cleaning the glassy carbon electrode with secondary distilled water to obtain the electrochemical sensor. The sensor is used in combination with an electrochemical workstation for 1.0 × 10-10 mol/L ~1.0×10-4 The samples with mol/L concentration are respectively detected.
Example 4
A preparation method of an electrochemical sensor for dopamine detection comprises the following steps:
polishing the glassy carbon electrode, and then ultrasonically cleaning in secondary distilled water;
the cleaned glassy carbon electrode is put into phosphate buffer solution with pH =7.0, and cyclic scanning treatment is carried out for 20 periods at a scanning rate of 20mV/s within a potential range of-0.2V to + 2.0V;
placing the glassy carbon electrode subjected to cyclic scanning into a phosphate buffer solution containing 0.1mmol/L bromophenol blue, wherein the pH of the buffer solution =7.0, and performing cyclic scanning again at a scanning speed of 20mV/s within a potential range of 0.0V to + 1.0V for 30 periods;
taking out the glassy carbon electrode subjected to the secondary circulating scanning treatment, washing the glassy carbon electrode by using secondary distilled water, and then putting the glassy carbon electrode into a phosphate buffer solution with the pH =5.0, wherein the potential range is from-0.2V to + 1.0V, and the circulating scanning treatment is carried out for 13 periods at the scanning speed of 15 mV/s;
taking out the glassy carbon electrode after cyclic scanning treatment, washing with secondary distilled water, and adding IrCl with a certain concentration of 0.2mmol/L30.1mmol/L of K2PtCl6And 0.2 mol/L of Na2SO4In the solution (a), the cyclic scanning treatment is performed again at a scanning rate of 15mV/s for 10 weeks in a potential range of-0.1V to + 1.0VA period;
and taking out the glassy carbon electrode subjected to the cyclic scanning treatment, and cleaning the glassy carbon electrode with secondary distilled water to obtain the electrochemical sensor. The sensor is used in combination with an electrochemical workstation for the treatment of dopamine-free and 1.0 × 10-10 mol/L ~1.0×10-4 Samples with mol/L concentration are respectively detected, a detection graph of the samples is shown in figure 1, and the electrochemical sensor shows good sensitivity to dopamine.
Claims (3)
1. A method for preparing an electrochemical sensor for detecting dopamine is characterized in that the electrochemical sensor comprises: the electrode comprises an electrode substrate, a modification layer and nano particles, wherein the electrode substrate is a glassy carbon electrode made of glassy carbon; the chemical modification layer is a bromophenol blue layer; the nano particle is IrO2-Pt, comprising the steps of:
polishing the glassy carbon electrode, and then ultrasonically cleaning in secondary distilled water;
putting the cleaned glassy carbon electrode into phosphate buffer solution with pH = 5.0-7.0, and performing cyclic scanning treatment at a scanning rate of 15-20 mV/s within a potential range of-0.2V to + 2.0V for 10-20 periods;
placing the glassy carbon electrode subjected to cyclic scanning into a phosphate buffer solution containing 0.1-1.0 mmol/L bromophenol blue, wherein the pH of the buffer solution is = 5.0-7.0, and carrying out cyclic scanning again at a scanning speed of 15-20 mV/s within a potential range of-1.0V to + 1.8V for 6-30 periods;
taking out the glassy carbon electrode after the cyclic scanning treatment, cleaning the glassy carbon electrode with secondary distilled water, and putting IrCl with a certain concentration3，K2PtCl6And Na2SO4In the solution, cyclic scanning is carried out again for 6-40 periods at a scanning speed of 5-20 mV/s within a certain potential range;
taking out the glassy carbon electrode subjected to the cyclic scanning treatment, and cleaning the glassy carbon electrode with secondary distilled water to prepare the electrochemical sensor; wherein the content of the first and second substances,
the IrCl3、 K2PtCl6And Na2SO4The concentration is 0.1 to 1.0 mmol/L.
2. An electrochemical sensor for dopamine detection, characterized in that it is prepared according to the method of claim 1.
3. Use of an electrochemical sensor according to claim 2 for dopamine detection.
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| EP0471330A1 (en) * | 1990-08-15 | 1992-02-19 | Degussa Ag | PH-measuring device |
| CN103031246A (en) * | 2011-10-10 | 2013-04-10 | 中国科学院电子学研究所 | Microelectrode array chip for multi-parameter detection of nerve cells and preparation method thereof |
| CN104076072A (en) * | 2014-06-25 | 2014-10-01 | 复旦大学 | High-sensitivity photoelectrochemical sensor made from iridium oxide-ferriporphyrin-titanium oxide and preparation method for sensor |
| CN105259224A (en) * | 2015-10-16 | 2016-01-20 | 上海纳米技术及应用国家工程研究中心有限公司 | Preparation method of electrochemical sensor for quick detection of dopamine |
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| EP0471330A1 (en) * | 1990-08-15 | 1992-02-19 | Degussa Ag | PH-measuring device |
| CN103031246A (en) * | 2011-10-10 | 2013-04-10 | 中国科学院电子学研究所 | Microelectrode array chip for multi-parameter detection of nerve cells and preparation method thereof |
| CN104076072A (en) * | 2014-06-25 | 2014-10-01 | 复旦大学 | High-sensitivity photoelectrochemical sensor made from iridium oxide-ferriporphyrin-titanium oxide and preparation method for sensor |
| CN105259224A (en) * | 2015-10-16 | 2016-01-20 | 上海纳米技术及应用国家工程研究中心有限公司 | Preparation method of electrochemical sensor for quick detection of dopamine |
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