CN109999010A - A kind of probiotics coating pellet and preparation method thereof - Google Patents
A kind of probiotics coating pellet and preparation method thereof Download PDFInfo
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- CN109999010A CN109999010A CN201910327539.0A CN201910327539A CN109999010A CN 109999010 A CN109999010 A CN 109999010A CN 201910327539 A CN201910327539 A CN 201910327539A CN 109999010 A CN109999010 A CN 109999010A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/742—Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/235—Foeniculum (fennel)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/42—Proteins; Polypeptides; Degradation products thereof; Derivatives thereof, e.g. albumin, gelatin or zein
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
- A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
- A61K9/5005—Wall or coating material
- A61K9/5021—Organic macromolecular compounds
- A61K9/5026—Organic macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyvinyl pyrrolidone, poly(meth)acrylates
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
- A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
- A61K9/5005—Wall or coating material
- A61K9/5021—Organic macromolecular compounds
- A61K9/5036—Polysaccharides, e.g. gums, alginate; Cyclodextrin
- A61K9/5042—Cellulose; Cellulose derivatives, e.g. phthalate or acetate succinate esters of hydroxypropyl methylcellulose
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
Abstract
The present invention relates to a kind of probiotics coating pellets and preparation method thereof, and the pellet includes each raw material of following parts by weight: 1 ~ 3 part of probiotics；11.5 ~ 17.5 parts of growth of probiotics promotor；2.5-5 parts of buckwheat protein isolate；10-35 parts of disintegrating agent；5-10 parts of adhesive；1-15 parts of filler.Preparation method includes the following steps: 1, actication of culture: 2, by after activation enterococcus faecium and bacillus subtilis be inoculated in fluid nutrient medium respectively, cultivate 16h after；Precipitating is collected by centrifugation, growth of probiotics promotor is added, obtains bacterium mud；3, water is added into buckwheat protein isolate, obtains protection liquid；4, bacterium mud, protection liquid and the mixing of other auxiliary materials, prepare softwood；5, granulation, ball blast；6, it is coated.Present invention is generally directed to cholates in gastric acid in external environment, stomach, gall-bladder to carry out crucial point protection to the destruction of probiotics, ensure that after reaching enteron aisle the effectively quantity of probiotics and promotes its field planting in enteron aisle.
Description
Technical field
The invention belongs to field of traditional Chinese veterinary, and in particular to a kind of probiotics coating pellet and preparation method thereof.
Background technique
Now, probiotics either in people's medication or veterinary medicine in occupation of biggish market, in body
Many aspects play more apparent adjustment effect, especially disease of digestive tract.Probiotics is active microorganism, they can only be
It survives in relatively limited environment, such as nutritional condition, anaerobic environment, preference temperature.When disengaging suitable growth environment, probiotics
Vigor reduces, is even dead.However the workplace of probiotics is the large intestine of animal, only more, the energetic probiotics of quantity
Large intestine is got to, its due biological effect is played.Therefore, the application of probiotics preparation has a three big difficult points, and one, stability asks
Topic: probiotic products are active microorganisms, are easy dead inactivation in preparation production, product preservation, use process；Two, prebiotic
Bacterium product is active microorganism, after detoxification, is needed after stomach, small intestine, and suitable growth environment -- large intestine is reached；By stomach
The effect of middle gastric acid and small intestine leading portion bile, most of viable bacteria vigor reduce, are even dead, therefore, even if taking viable bacteria, if
Lack safeguard measure appropriate, not can guarantee it can smoothly reach large intestine and non-inactivation；Three, it is present in animal large intestine natural
The microbial flora of the substantial amounts of growth, in large intestine in the competitive growth of microbial flora, external source probio is difficult to fall
Ground is taken root, and there was only that quantity is more, energetic and nutritional sufficiency, could be bred and be generated a large amount of metabolites rapidly, play its life
Object effect.
Summary of the invention
It is an object of the invention to overcome the deficiencies of existing technologies, a kind of probiotics coating pellet and its preparation side are provided
Method.
To achieve the goals above, the technical solution adopted by the present invention is as follows:
Technical solution one:
A kind of probiotics coating pellet, including each raw material of following parts by weight in terms of dry weight:
The living bacteria count of the probiotics: every gram is more than or equal to 1 × 1011It is a.
Further, the growth of probiotics promotor include one of tawny daylily root extract and sweet fennel extract or
Two kinds.
Further, the growth of probiotics promotor by 10-15 parts by weight tawny daylily root extract and 1.5-2.5 weight
The sweet fennel extract of part compounds.
Further, the probiotics includes one or both of enterococcus faecium and bacillus subtilis.
Further, the mass ratio of the enterococcus faecium and bacillus subtilis is 1:1；Every gram of enterococcus faecium and withered
The living bacteria count of careless bacillus is all larger than equal to 1 × 1011It is a.
Further, the disintegrating agent includes one or both of microcrystalline cellulose, sodium carboxymethylcellulose.
Further, described adhesive includes one or both of bletilla polysaccharide, chitosan.
Further, the filler includes one or both of glucose, starch.
Technical solution two
A kind of preparation method of probiotics coating pellet, specifically comprises the following steps:
Step 1, actication of culture: enterococcus faecium and bacillus subtilis are inoculated in by the way of streak inoculation respectively
On activation medium, in 37 degrees Celsius of activation culture 16h；
Step 2, bacterium mud preparation: by after activation enterococcus faecium and bacillus subtilis respectively at being expanded in fluid nutrient medium
Culture, then respectively by bacterium solution to be centrifuged 10min under the revolving speed of 5000r/min, after collecting precipitating, by enterococcus faecium and withered grass bud
After the precipitating mixing of both spore bacillus, growth of probiotics promotor is added thereto, obtains bacterium mud, it is spare；
When amplification cultivation, the inoculum concentration of enterococcus faecium is 2%, condition of culture are as follows: amplification cultivation under 37 degrees celsius
16h；
When amplification cultivation, the inoculum concentration of bacillus subtilis is 1.5%, condition of culture are as follows: expand under 37 degrees celsius
Cultivate 48h；
Step 3, the preparation for protecting liquid: pressing material liquid volume ratio 1:2~3, and water is added into buckwheat protein isolate, and mixing is equal
It is even, protection liquid is obtained, it is spare；
Step 4 prepares softwood: protection liquid made from step 3 is added in bacterium mud made from step 2, after mixing,
Disintegrating agent, adhesive and filler is added, softwood is made；
Step 5, granulation, ball blast: after the softwood prepared is squeezed granulation, 40 meshes is crossed, drug granule is obtained；Then it uses
Shot-blasting machine with the speed of 150 turns/min carry out ball blast to the ball heart it is round as a ball after, fluidized drying 60 minutes, be naturally cooling to 35 DEG C, obtain
The ball heart；
Step 6, coating: being dissolved with solvent by coating auxiliary material first, prepare coating solution, then, the ball heart is packed into multi-functional
Fluidized-bed coating machine, after air outlet temperature rises to 40 DEG C, by the ball heart and coating auxiliary material dry weight than 3~9:1, by coating solution
Gun spraying coating is squeezed into through peristaltic pump, coating process air outlet temperature is kept for 45 DEG C or so；Coating finishes, and continues at multi-functional
Fluidized-bed coating machine keeps being dried for 15 minutes.
Further, the formula of fluid nutrient medium described in step 2 are as follows: beef extract 0.5g, peptone 1.0g, sodium chloride
0.5g, distilled water 100mL, pH7.2~7.5.
Further, the mass ratio of the coating auxiliary material and the solvent is 5~10:85~90；The solvent is by anhydrous
30:55~60 are uniformly mixed and are made by volume for ethyl alcohol and water.
Further, the coating auxiliary material includes hydroxypropyl methyl cellulose phthalate/3-8 parts of polyacrylic resin；Lemon
Lemon triethylenetetraminehexaacetic acid ester/1-3 parts of propylene glycol；1-2 parts of titanium dioxide.
Compared with prior art, the beneficial effects of the present invention are:
1, the present invention enables probiotics to be disintegrated release in enteron aisle, solves probiotics in stomach by enteric coating technology
Unstable problem in acid.
2, the present invention uses protective agent of the buckwheat protein isolate as probiotics, and probiotics can be protected from enteron aisle height
Influence under cholic acid salt environment can survive and breed under the intestinal environment of high cholate.
3, the present invention, can using tawny daylily root extract and/or the growth promoter of sweet fennel extract effect probiotics
Promote the growth and breeding of probiotics, but not has to the harmful bacteria in enteron aisle and promote numerous effect, therefore promote probiotics in enteron aisle
Interior field planting.
It is each from causing probiotics to inactivate that present invention employs probiotics protective agent, growth promoter and enteric coating technologies
Link protects probiotics, ensure that after reaching enteron aisle the effectively quantity of probiotics and promotes it and determine in enteron aisle
It plants, so that probiotics be enable to play the effect of it should have.
Specific embodiment
Further details of narration is carried out to the present invention with reference to embodiments.
Enterococcus faecium: Shanghai Hui Ying Biotechnology Co., Ltd；Every gram contains viable bacteria 1 × 1011More than.
Bacillus subtilis: Shanghai Hui Ying Biotechnology Co., Ltd；Every gram contains viable bacteria 1 × 1011More than.
Tawny daylily root extract: Sichuan Chengdu Tian Yuan plant extracts Co., Ltd.
Buckwheat protein isolate: Sichuan Chengdu Tian Yuan plant extracts Co., Ltd.
Sweet fennel extract: it by fennel seeds with water boiling and extraction 2 times, after merging No. 2 extracting solutions, is concentrated under reduced pressure, is spraying dry
It is dry to obtain the final product.
Embodiment 1
A kind of probiotics coating pellet, the ball heart is in terms of dry weight including each raw material of following weight:
Coating is compounded by coating auxiliary material with solvent 8:87.5 in mass ratio；
Coating auxiliary material includes each raw material of following weight:
Hydroxypropyl methyl cellulose phthalate 5Kg；
Triethyl citrate 2Kg；
Titanium dioxide 1.5Kg；
By dehydrated alcohol and water, 30:57.5 is uniformly mixed and is made the solvent by volume.
Preparation method are as follows:
Step 1, actication of culture: enterococcus faecium and bacillus subtilis are inoculated in by the way of streak inoculation respectively
On activation medium, in 37 degrees Celsius of activation culture 16h；
Step 2, bacterium mud preparation:
Enterococcus faecium after activation is inoculated in fluid nutrient medium by 2% inoculum concentration, is expanded under 37 degrees celsius
By bacterium solution to be centrifuged 10min under the revolving speed of 5000r/min after culture 16h, enterococcus faecium precipitating is collected；
Bacillus subtilis after activation is inoculated in fluid nutrient medium by 1.5% inoculum concentration, in 37 degrees celsius
After lower amplification cultivation 48h (to obtain 90% or more withered grass gemma), by bacterium solution to be centrifuged under the revolving speed of 5000r/min
10min collects bacillus subtilis precipitating；After enterococcus faecium precipitating and bacillus subtilis precipitating mixing, it is added thereto
Growth of probiotics promotor obtains bacterium mud, spare；
Step 3, the preparation for protecting liquid: pressing material liquid volume ratio 1:2~3, and water is added into buckwheat protein isolate, and mixing is equal
It is even, protection liquid is obtained, it is spare；
Step 4 prepares softwood: protection liquid made from step 3 is added in bacterium mud made from step 2, after mixing,
Disintegrating agent, adhesive and filler is added, softwood is made；
Step 5, granulation, ball blast: after the softwood prepared is squeezed granulation, 40 meshes is crossed, drug granule is obtained；Then it uses
Shot-blasting machine with the speed of 150 turns/min carry out ball blast to the ball heart it is round as a ball after, fluidized drying 60 minutes, be naturally cooling to 35 DEG C, obtain
The ball heart；
Step 6, coating:
Step 6.1 weighs hydroxypropyl methyl cellulose phthalate, triethyl citrate, titanium dioxide by weight, and mixing is equal
After even, coating auxiliary material is obtained；
Step 6.2, by coating auxiliary material and solvent quality ratio 8:87.5, will coating auxiliary material solvent dissolve (solvent by
Dehydrated alcohol and water by volume 30:57.5 be uniformly mixed be made), prepare coating solution；
The ball heart is packed into multifunctional fluidized bed seed-coating machine by step 6.3, after air outlet temperature rises to 40 DEG C, by the ball heart and
Auxiliary material dry weight is coated than 3~9:1, and coating solution is squeezed into gun spraying coating, coating process air outlet temperature through peristaltic pump
Kept for 45 DEG C or so；Coating finishes, and continues at multifunctional fluidized bed seed-coating machine and keeps being dried for 15 minutes.
Embodiment 2
A kind of probiotics coating pellet, the ball heart is in terms of dry weight including each raw material of following weight:
Coating is compounded by coating auxiliary material with solvent 8:87.5 in mass ratio；
Coating auxiliary material includes each raw material of following weight:
Hydroxypropyl methyl cellulose phthalate 5Kg；
Triethyl citrate 2Kg；
Titanium dioxide 1.5Kg；
By dehydrated alcohol and water, 30:57.5 is uniformly mixed and is made the solvent by volume.
Preparation method are as follows: with embodiment 1.
Embodiment 3
A kind of probiotics coating pellet, the ball heart is in terms of dry weight including each raw material of following weight:
Coating is compounded by coating auxiliary material with solvent 8:87.5 in mass ratio；
Coating auxiliary material includes each raw material of following weight:
Hydroxypropyl methyl cellulose phthalate 5Kg；
Triethyl citrate 2Kg；
Titanium dioxide 1.5Kg；
By dehydrated alcohol and water, 30:57.5 is uniformly mixed and is made the solvent by volume.
Preparation method are as follows: with embodiment 1.
Embodiment 4
A kind of probiotics coating pellet, ball pericardium include each raw material of following weight Kg in terms of dry weight:
Coating is compounded by coating auxiliary material with solvent 8:87.5 in mass ratio；
Coating auxiliary material includes each raw material of following weight:
Hydroxypropyl methyl cellulose phthalate 5Kg；
Triethyl citrate 2Kg；
Titanium dioxide 1.5Kg；
By dehydrated alcohol and water, 30:57.5 is uniformly mixed and is made the solvent by volume.
Preparation method are as follows: with embodiment 1.
Embodiment 5
A kind of probiotics coating pellet, the ball heart is in terms of dry weight including each raw material of following weight:
Coating is compounded by coating auxiliary material with solvent 8:87.5 in mass ratio；
Coating auxiliary material includes each raw material of following weight:
Hydroxypropyl methyl cellulose phthalate 5Kg；
Triethyl citrate 2Kg；
Titanium dioxide 1.5Kg；
By dehydrated alcohol and water, 30:57.5 is uniformly mixed and is made the solvent by volume.
Preparation method are as follows: with embodiment 1.
Comparative example 1
A kind of probiotics, including each raw material of following weight in terms of dry weight:
Enterococcus faecium 1Kg；
Bacillus subtilis 1Kg；
Preparation method are as follows: step 1, actication of culture: enterococcus faecium and bacillus subtilis are used into streak inoculation respectively
Mode, be inoculated on activation medium, in 37 degrees Celsius of activation culture 16h；
Step 2, bacterium mud preparation:
Enterococcus faecium after activation is inoculated in fluid nutrient medium by 2% inoculum concentration, is expanded under 37 degrees celsius
By bacterium solution to be centrifuged 10min under the revolving speed of 5000r/min after culture 16h, enterococcus faecium precipitating is collected, dung intestines ball is spray-dried to obtain
Bacterium；
Bacillus subtilis after activation is inoculated in fluid nutrient medium by 1.5% inoculum concentration, in 37 degrees celsius
After lower amplification cultivation 48h (to obtain 90% or more withered grass gemma), by bacterium solution to be centrifuged under the revolving speed of 5000r/min
10min collects bacillus subtilis precipitating, is spray-dried to obtain bacillus subtilis；
Step 3 after being weighed enterococcus faecium, bacillus subtilis in terms of dry weight, is uniformly mixed, (can be stood after mixing
Carry out every effect test).
Effect example 1: influence of the probiotics growth-promoting agent to growth of probiotics performance
One, test method:
1, the growth curve of enterococcus faecium
Take 36 triangular flasks, be divided into 3 groups, by grouping situation be packed into fluid nutrient medium, every group 12 bottles, respectively number be
1-12；The enterococcus faecium bacteria suspension after activating will be taken, is inoculated in each bottle culture solution respectively by 5% inoculum concentration, respectively at 37
After DEG C CMC model 0,1,2,4,6,8,9,10,11,12h, 14,16h, the measurement of OD (600nm) is carried out, data are shown in Table 1, then
Draw growth curve.
It is grouped situation:
Control group: basal medium；
Basal medium formulation are as follows: peptone 10.0g, sodium chloride 5.0g, beef extract powder 3.0g, distilled water 1.0L, pH7.2
± 0.2,121 DEG C of high pressure sterilization 15min are spare；
Tawny daylily root extract group: tawny daylily root extract, which is added, into basal medium makes tawny daylily root extract in culture medium
Final mass concentration reaches 20%, pH7.2 ± 0.2, and 121 DEG C of high pressure sterilization 15min are spare；
Sweet fennel extract group: sweet fennel extract, which is added, into basal medium makes medium anise extract
Final mass concentration reaches 20%.
2, the growth curve of bacillus subtilis
Take 36 triangular flasks, be divided into 3 groups, by grouping situation be packed into fluid nutrient medium, every group 12 bottles, respectively number be
1-12；The bacillus subtilis bacteria suspension after activating will be taken, be inoculated in each bottle culture solution respectively by 5% inoculum concentration, respectively
After 37 DEG C of CMC models 0,1,2,4,6,8,9,10,11,12h, 14,16h, the measurement of OD (600nm) is carried out, data are shown in Table 1,
Then growth curve is drawn.
It is grouped situation:
Control group: basal medium；
Basal medium formulation are as follows: peptone 10.0g, sodium chloride 5.0g, beef extract powder 3.0g, distilled water 1.0L, pH7.2
± 0.2,121 DEG C of high pressure sterilization 15min are spare；
Tawny daylily root extract group: tawny daylily root extract, which is added, into basal medium makes tawny daylily root extract in culture medium
Final mass concentration reaches 20%, pH7.2 ± 0.2, and 121 DEG C of high pressure sterilization 15min are spare；
Sweet fennel extract group: sweet fennel extract, which is added, into basal medium makes medium anise extract
Final mass concentration reaches 20%,
The growing state of table 1 enterococcus faecium and bacillus subtilis
Influence of the 2 probiotics growth-promoting agent of effect example to harmful bacteria growth performance
1, the growth curve of Escherichia coli
Take 36 triangular flasks, be divided into 3 groups, by grouping situation be packed into fluid nutrient medium, every group 12 bottles, respectively number be
1-12；The Escherichia coli bacteria suspension after activating will be taken, is inoculated in each bottle culture solution respectively by 5% inoculum concentration, respectively at 37
After DEG C CMC model 0,1,2,4,6,8,9,10,11,12h, 14,16h, the measurement of OD (600nm) is carried out, data are shown in Table 2, then
Draw growth curve.
It is grouped situation:
Control group: basal medium；
Basal medium formulation are as follows: peptone 10.0g, sodium chloride 5.0g, beef extract powder 3.0g, distilled water 1.0L, pH7.2
± 0.2,121 DEG C of high pressure sterilization 15min are spare；
Tawny daylily root extract group: tawny daylily root extract, which is added, into basal medium makes tawny daylily root extract in culture medium
Final mass concentration reaches 20%, pH7.2 ± 0.2, and 121 DEG C of high pressure sterilization 15min are spare；
Sweet fennel extract group: sweet fennel extract, which is added, into basal medium makes medium anise extract
Final mass concentration reaches 20%；
2, the growth curve of salmonella
Take 36 triangular flasks, be divided into 3 groups, by grouping situation be packed into fluid nutrient medium, every group 12 bottles, respectively number be
1-12；The salmonella bacteria suspension after activating will be taken, is inoculated in each bottle culture solution respectively by 5% inoculum concentration, respectively at 37
After DEG C CMC model 0,1,2,4,6,8,9,10,11,12h, 14,16h, the measurement of OD (600nm) is carried out, data are shown in Table 2, then
Draw growth curve.
It is grouped situation:
Control group: basal medium；
Basal medium formulation are as follows: peptone 10.0g, sodium chloride 5.0g, beef extract powder 3.0g, distilled water 1.0L, pH7.2
± 0.2,121 DEG C of high pressure sterilization 15min are spare；
Tawny daylily root extract group: tawny daylily root extract, which is added, into basal medium makes tawny daylily root extract in culture medium
Final mass concentration reaches 20%, pH7.2 ± 0.2, and 121 DEG C of high pressure sterilization 15min are spare；
Sweet fennel extract group: sweet fennel extract, which is added, into basal medium makes medium anise extract
Final mass concentration reaches 20%.
The growing state of table 2 Escherichia coli and salmonella
Protective effect of the 3 buckwheat protein isolate of effect example to probiotics
One, enterococcus faecium
If the cholate MRS culture medium of different quality containing, respectively 0.00%, 0.05%, 0.10%, 0.5%,
1.00%, 1.5%；Adjusting bacterial strain number in each culture medium is 1.0 × 103A/ml is inoculated with respectively in above-mentioned cholate medium,
It is divided into two groups simultaneously, while is divided into two groups, 3.5% buckwheat test group: is added in the cholate MRS culture medium of different quality containing
Wheat protein isolate, control group: the cholate MRS culture medium of different quality containing；In 37 DEG C of constant-temperatureanaerobic anaerobic culture medium stationary cultures,
12h is cultivated, after taking culture solution to dilute suitable concentration, the enterococcus faecium in fermentation liquid is counted using colony counting method,
And increment multiple is calculated, each group is repeated 3 times, and is finally averaged, data are shown in Table 3.
Two, bacillus subtilis
If the cholate MRS culture medium of different quality containing, respectively 0.00%, 0.05%, 0.10%, 0.5%,
1.00%, 1.5%；Adjusting bacterial strain number in each culture medium is 1.0 × 103A/ml is inoculated with respectively in above-mentioned cholate medium,
It is divided into two groups simultaneously, test group: being added 3.5% buckwheat protein isolate in the cholate MRS culture medium of different quality containing, right
According to group: the cholate MRS culture medium of different quality containing；In 37 DEG C of constant-temperatureanaerobic anaerobic culture medium stationary cultures, 12h is cultivated, training is taken
After nutrient solution dilutes suitable concentration, the bacillus subtilis in fermentation liquid is counted using colony counting method, each group of weight
It is 3 times multiple, it is finally averaged, data are shown in Table 4.
Increment situation of 3 enterococcus faecium of table behind culture 12 hours in various concentration cholate
Increment situation of 4 bacillus subtilis of table behind culture 12 hours in the cholate of various concentration
4 clinical pharmacology of effect example test: influence of the probiotics to Stock genetics and breeding
Experimental animal: the Yorkshire weanling pig of 23 ages in days, male and female is unlimited, amounts to 125.
125 weanling pigs are randomly divided into five groups, respectively test 1 group, test 2 groups, 3 groups of test, control 1 group and yin
Property control group, every group of daily management and feed all the same, observe every group of diarrhea rate, final feedstuff-meat ratio and average weight gain feelings
Condition the results are shown in Table 5.
Grouping situation: 1 group of test: the coating pellet of probiotics made from embodiment 1 is added into feed feeding, with prebiotic
Bacterium dry weight meter, feed addition probiotics 30g per ton, continuous feeding 25 days；
It tests 2 groups: the coating pellet of probiotics made from embodiment 4 is added into feed feeding, in terms of probiotics dry weight,
Feed addition probiotics 30g per ton, continuous feeding 25 days；
It tests 3 groups: the coating pellet of probiotics made from embodiment 5 is added into feed feeding, in terms of probiotics dry weight,
Feed addition probiotics 30g per ton, continuous feeding 25 days；
It compares 1 group: probiotics made from comparative example 1 is added into feed feeding, in terms of probiotics dry weight, feed per ton
Add probiotics 30g, continuous feeding 25 days；
Negative control group: not any probiotics of feeding.
Table 5
| Feedstuff-meat ratio | Average weight gain (Kg) | Diarrhea rate (%) | |
| Test 1 group | 1.39 | 7.62 | 1.07 |
| Test 2 groups | 1.36 | 7.85 | 1.02 |
| Test 3 groups | 1.38 | 7.72 | 1.05 |
| Compare 1 group | 1.54 | 6.69 | 3.56 |
| Negative control group | 1.58 | 6.35 | 6.07 |
Embodiment described above is merely a preferred embodiment of the present invention, and the simultaneously exhaustion of the feasible implementation of non-present invention.It is right
For persons skilled in the art, any aobvious to made by it under the premise of without departing substantially from the principle of the invention and spirit and
The change being clear to should be all contemplated as falling within claims of the invention.
Claims (9)
1. a kind of probiotics is coated with pellet, which is characterized in that including each raw material of following parts by weight in terms of dry weight:
1 ~ 3 part of probiotics；
11.5 ~ 17.5 parts of growth of probiotics promotor；
2.5-5 parts of buckwheat protein isolate；
10-35 parts of disintegrating agent；
5-10 parts of adhesive；
1-15 parts of filler；
The living bacteria count of the probiotics: every gram is more than or equal to 1 × 1011It is a.
2. a kind of probiotics according to claim 1 is coated with pellet, which is characterized in that the growth of probiotics promotor packet
Include one or both of tawny daylily root extract and sweet fennel extract.
3. a kind of probiotics according to claim 1 is coated with pellet, which is characterized in that the probiotics includes enterococcus faecium
One or both of with bacillus subtilis.
4. a kind of probiotics according to claim 1 is coated with pellet, which is characterized in that the disintegrating agent includes crystallite fibre
One or both of dimension, sodium carboxymethylcellulose.
5. a kind of probiotics according to claim 1 is coated with pellet, which is characterized in that described adhesive includes that the bletilla striata is more
One or both of sugar, chitosan.
6. a kind of probiotics according to claim 1 is coated with pellet, which is characterized in that the filler include glucose,
One or both of starch.
7. a kind of preparation method for being coated with pellet such as any one of claim 1 ~ 6 probiotics, which is characterized in that specifically include
Following steps:
Step 1, actication of culture: respectively by enterococcus faecium and bacillus subtilis by the way of streak inoculation, it is inoculated in activation
On culture medium, in 37 degrees Celsius of activation culture 16h；
Step 2, bacterium mud preparation: by after activation enterococcus faecium and bacillus subtilis respectively in fluid nutrient medium expand train
It supports, then respectively by bacterium solution to be centrifuged 10min under the revolving speed of 5000r/min, after collecting precipitating, by enterococcus faecium and withered grass gemma
After the precipitating mixing of both bacillus, growth of probiotics promotor is added thereto, obtains bacterium mud, it is spare；
When amplification cultivation, the inoculum concentration of enterococcus faecium is 2%, condition of culture are as follows: amplification cultivation 16h under 37 degrees celsius；
When amplification cultivation, the inoculum concentration of bacillus subtilis is 1.5%, condition of culture are as follows: amplification cultivation under 37 degrees celsius
48h；
Step 3, the preparation for protecting liquid: material liquid volume ratio 1:2 ~ 3 are pressed, water is added into buckwheat protein isolate, is uniformly mixed, must protect
Liquid is protected, it is spare；
Step 4 prepares softwood: protection liquid made from step 3 being added in bacterium mud made from step 2, after mixing, is added
Disintegrating agent, adhesive and filler, are made softwood；
Step 5, granulation, ball blast: after the softwood prepared is squeezed granulation, 40 meshes is crossed, drug granule is obtained；Then ball blast is used
Machine with the speed of 150 turns/min carry out ball blast to the ball heart it is round as a ball after, fluidized drying 60 minutes, be naturally cooling to 35 DEG C, obtain the ball heart；
Step 6, coating: being dissolved with solvent by coating auxiliary material first, prepare coating solution, then, the ball heart is packed into multi-functional fluidisation
Bed seed-coating machine, after air outlet temperature rises to 40 DEG C, by the ball heart and coating auxiliary material dry weight than 3 ~ 9:1, by coating solution through compacted
Dynamic pump squeezes into gun spraying coating, and coating process air outlet temperature is kept for 45 DEG C or so；Coating finishes, and continues at multi-functional fluidisation
Bed seed-coating machine keeps being dried for 15 minutes.
8. a kind of probiotics according to claim 7 is coated with pellet, which is characterized in that the coating auxiliary material and the solvent
Mass ratio be 5 ~ 10:85 ~ 90；By dehydrated alcohol and water, 30:55 ~ 60 are uniformly mixed and are made the solvent by volume.
9. a kind of probiotics according to claim 8 is coated with pellet, which is characterized in that the coating auxiliary material includes hydroxypropyl
Methylcellulose phthalate ester/3-8 parts of polyacrylic resin；Triethyl citrate/1-3 parts of propylene glycol；1-2 parts of titanium dioxide.
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Citations (5)
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| CN101537020A (en) * | 2009-04-24 | 2009-09-23 | 东北制药集团公司沈阳第一制药厂 | Synbiotics of bacillus licheniformis and oligosaccharide class prebiotics and composition and formulation thereof |
| WO2014152338A1 (en) * | 2013-03-14 | 2014-09-25 | Kabadi Mohan | Targeted gastrointestinal tract delivery of probiotic organisms and/or therapeutic agents |
| WO2015121458A2 (en) * | 2014-02-14 | 2015-08-20 | Vesale Pharma Sa | Composition comprising bifidobacterium animalis ssp. lactis |
| CN109464425A (en) * | 2018-12-29 | 2019-03-15 | 上海交大昂立股份有限公司 | A kind of probiotics embedded particles and preparation method thereof |
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2019
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| CN101537020A (en) * | 2009-04-24 | 2009-09-23 | 东北制药集团公司沈阳第一制药厂 | Synbiotics of bacillus licheniformis and oligosaccharide class prebiotics and composition and formulation thereof |
| WO2014152338A1 (en) * | 2013-03-14 | 2014-09-25 | Kabadi Mohan | Targeted gastrointestinal tract delivery of probiotic organisms and/or therapeutic agents |
| WO2015121458A2 (en) * | 2014-02-14 | 2015-08-20 | Vesale Pharma Sa | Composition comprising bifidobacterium animalis ssp. lactis |
| CN109464425A (en) * | 2018-12-29 | 2019-03-15 | 上海交大昂立股份有限公司 | A kind of probiotics embedded particles and preparation method thereof |
| CN109528691A (en) * | 2019-01-15 | 2019-03-29 | 中国农业科学院油料作物研究所 | Core-shell structure cellulose base probiotic microcapsule and preparation method thereof |
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