CN109997873A - A kind of agricultural chemicals suspension agent and preparation method thereof for preventing and treating prodenia litura - Google Patents
A kind of agricultural chemicals suspension agent and preparation method thereof for preventing and treating prodenia litura Download PDFInfo
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- CN109997873A CN109997873A CN201910217193.9A CN201910217193A CN109997873A CN 109997873 A CN109997873 A CN 109997873A CN 201910217193 A CN201910217193 A CN 201910217193A CN 109997873 A CN109997873 A CN 109997873A
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- prodenia litura
- agricultural chemicals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N53/00—Biocides, pest repellants or attractants, or plant growth regulators containing cyclopropane carboxylic acids or derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N55/00—Biocides, pest repellants or attractants, or plant growth regulators, containing organic compounds containing elements other than carbon, hydrogen, halogen, oxygen, nitrogen and sulfur
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
Abstract
The present invention provides a kind of agricultural chemicals suspension agents for preventing and treating prodenia litura, including following component by mass percentage: prodenia litura nuclear polyhedrosis virus 0.01~3,000,000,000 PIB/g, pyrethrin pesticide 0.1~30%, adjuvant 2.5~28.5%, surplus are deionized water.In addition, the present invention also provides the preparation methods of the agricultural chemicals suspension agent.The agricultural chemicals suspension agent of the invention compounds the principal component as pesticide using the reagent of two kinds of different role mechanism of pyrethrin pesticide and prodenia litura nuclear polyhedrosis virus, significant synergistic effect is shown to a certain extent, dosage can be reduced, be conducive to delay pest to the resistance of single medicament, to improve agricultural insecticide quick-acting, delaying drug resistance, extend the service life of pesticide, and environmentally friendly, it is highly-safe.
Description
Technical field
The invention belongs to technical field of pesticide, and in particular to it is a kind of prevent and treat prodenia litura agricultural chemicals suspension agent and its preparation side
Method.
Background technique
Prodenia litura is commonly called as lotus Autographa spp, agrotis, belongs to Lepidoptera, Noctuidae, is a kind of worldwide distribution, endangers host
Quite extensive omnivorousness and binge pest.In addition to brassicaceous vegetable, can also it endanger including melon, eggplant, beans, green onion, leek, spinach
Dish and grain, 100 section of industrial crops Deng Jin, 300 various plants.There are generation in various regions to prodenia litura at home, main to occur
In the Jiangxi of the Yangtze river basin, Jiangsu, Hunan, Hubei, Zhejiang, Anhui, Henan, Hebei, Shandong of Yellow River basin etc. are saved, the world
Property distribution, China in addition to Qinghai, Xinjiang are unknown, each province (autonomous region) is found.Its harmful characteristics: larva bites blade, flower
Flower bud, flower and fruit, first instar larvae nibble food blade lower epidermis and mesophyll, only stay the transparent spot of epicuticle；Enter gluttony after 4 ages,
Blade is bitten, only stays master pulse, feeding habits are not only miscellaneous but also each organ of causing harm, and form gluttony when aged, are a kind of highly harmful evils
Worm.
Method currently used for preventing and treating lepidoptera pest is mainly chemical insecticide, due to the worm generable generation every year
Number is more, and occurrence quantity is big, easy population outbreak, and many a large amount of Reusability chemical insecticides in area, control efficiency is poor, and resistance is promoted
Obviously, and to environment very big influence is brought.Using prevention and cure of viruses pest, not only to safety of human and livestock, be conducive to protect day
Enemy and ecological environment, and pest is not easy to produce resistance.In addition, virus in pest population can form epidemic disease and long-term
Insect density is controlled, there are obvious economy and ecological benefits.But prevention and cure of viruses in practical applications by quick-acting not
By force, the influence for the defects of preparation is unstable.
Summary of the invention
The purpose of the present invention is overcome existing prevention and treatment prodenia litura chemical pesticide ingredient is single, resistance is high, and biology disease
Toxin preparation is unstable, quick-acting is not strong, leads to the problem of control efficiency difference.
For this purpose, the present invention provides a kind of agricultural chemicals suspension agent for preventing and treating prodenia litura, including by mass percentage as
Lower component: prodenia litura nuclear polyhedrosis virus 0.01~3,000,000,000 PIB/g, pyrethrin pesticide 0.1~30%, adjuvant 2.5~
28.5%, surplus is deionized water.
Further, the pyrethrin pesticide includes lambda-cyhalothrin, gamma cyhalothrin, cypermethrin, efficient chlorine
At least one of Cyano chrysanthemate, Fenpropathrin, Biphenthrin, decis, silafluofene, prallethrin and etrafluorine ethofenprox.
Further, the pyrethrin pesticide include gamma cyhalothrin, effective cypermethrin, in Biphenthrin extremely
Few one kind.
Further, the adjuvant includes dispersing agent, wetting agent, thickener, antifreezing agent, defoaming agent and preservative, and
The degree that each component accounts for agricultural chemicals suspension agent gross mass is as follows: dispersing agent 1.0~8.0%, wetting agent 0.5~8.0%,
Thickener 0.05~2.0%, antifreezing agent 1.0~10.0%, defoaming agent 0.1~0.5%, preservative 0.1~2.0%.
As one of embodiment, the agricultural chemicals suspension agent of the prevention and treatment prodenia litura includes by mass percentage as follows
Component: prodenia litura nuclear polyhedrosis virus 0.01~2,000,000,000 PIB/g, pyrethrin pesticide 0.2~25%, sodium benzoate 0.2~
1.0%, alkyl phenol polyethenoxy ether sodium sulfate salt 1.0~5.0%, polyoxyethylene polyoxypropylene block copolymer 1.0~
5.0%, xanthan gum 0.05~2.0%, ethylene glycol 3.0~10.0%, organic silicon defoamer 0.1~0.5%, surplus be go from
Sub- water.
As one of embodiment, the agricultural chemicals suspension agent of the prevention and treatment prodenia litura includes by mass percentage as follows
Component: prodenia litura nuclear polyhedrosis virus 0.02~1,000,000,000 PIB/g, pyrethrin pesticide 0.5~20%, sodium benzoate 0.2~
0.8%, polycarboxylate 1.0~5.0%, the polyether-modified object 0.5~4.0% of modified rosin, xanthan gum 0.05~2.0%, the third two
Alcohol 3.0~10.0%, organic silicon defoamer 0.1~0.5%, surplus are deionized water.
As one of embodiment, the agricultural chemicals suspension agent of the prevention and treatment prodenia litura includes by mass percentage as follows
Component: prodenia litura nuclear polyhedrosis virus 0.02~500,000,000 PIB/g, pyrethrin pesticide 0.5~15%, sodium benzoate 0.2~
0.8%, phosphate 1.0~3.0%, polycarboxylate 0.5~3.0%, polyoxyethylene polyoxypropylene block copolymer 0.5~
4.0%, xanthan gum 0.05~2.0%, glycerol 3.0~10.0%, organic silicon defoamer 0.1~0.5%, surplus is deionization
Water.
As one of embodiment, the agricultural chemicals suspension agent of the prevention and treatment prodenia litura includes by mass percentage as follows
Component: prodenia litura nuclear polyhedrosis virus 0.02~400,000,000 PIB/g, pyrethrin pesticide 0.8~12%, alkyl ethylsulfonic acid sodium
Salt 1.0~3.0%, polycarboxylate 1.0~4.0%, fatty alcohol polyoxyethylene ether 0.5~4.0%, xanthan gum 0.05~2.0%,
Propylene glycol 3.0~10.0%, organic silicon defoamer 0.1~0.5%, surplus are deionized water.
As one of embodiment, the agricultural chemicals suspension agent of the prevention and treatment prodenia litura includes by mass percentage as follows
Component: prodenia litura nuclear polyhedrosis virus 0.02~200,000,000 PIB/g, pyrethrin pesticide 1.0~10%, acrylic graft copolymer
Object 1.0~4.0%, polyoxyethylene polyoxypropylene block copolymer 0.5~4.0%, xanthan gum 0.05~2.0%, ethylene glycol
3.0~10.0%, organic silicon defoamer 0.1~0.5%, surplus is deionized water.
In addition, the present invention also provides the preparation methods of the agricultural chemicals suspension agent of above-mentioned prevention and treatment prodenia litura, including walk as follows
It is rapid:
1) prodenia litura nuclear polyhedrosis virus, pyrethrin pesticide, adjuvant are put into deionized water by design quantity
In, and 1h is sheared in high-speed shearing machine, obtain mixed liquor；
2) mixed liquor obtained in step 1) is sanded by the series connection of two sand mills, makes in mixed liquor diameter of particle in 5 μ
M hereinafter, by the suspension being sanded by pneumatic diaphragm be pumped into shearing homogeneous kettle in homogeneous 1h to get the agricultural-chemical suspension
Agent.
In the present invention pyrethrin pesticide have stomach toxicity and action of contace poison, can by with pest (prodenia litura) sodium channel
Interact and destroy the function of its nervous system, have the characteristics that efficiently, wide spectrum, low toxicity, low-residual, but pyrethrin pesticide
A large amount of uses can make pest develop drug resistance.And prodenia litura nuclear polyhedrosis virus is oral or wound infection enters pest body quilt
Gastric juice digestion, dissociate baculovirus particle, enters body cavity by midgut epithelial cell, invades cell, be proliferated in nucleus,
Invade healthy cell again later, until pest is lethal, and disease pest excrement and dead worm can infect other healthy pests again, make virus
Disease is popular in pest population, while virus can also pass to pest filial generation by ovum, so that pest damage is controlled, but its desinsection
It composes that narrow, quick-acting is bad, preparation is unstable, is easy to be influenced and inactivate by ultraviolet light.The present invention is by pyrethrin pesticide and twill
Californica nuclear polyhedrosis virus is compounded, and the mechanism of action of two ingredients for pest control is different, can be mutually after mixture
Significant synergistic effect is shown to a certain extent, can reduce dosage, is conducive to delay pest to the resistance of single medicament,
To improve agricultural insecticide quick-acting, delaying drug resistance, extend the service life of pesticide, and environmentally friendly, it is highly-safe.
Compared with prior art, beneficial effects of the present invention:
(1) agricultural chemicals suspension agent of this prevention and treatment prodenia litura provided by the invention is using chemistry and biological two kinds of different roles
The reagent of mechanism compounds the principal component as pesticide, is conducive to delay pest to the resistance of single medicament, to improve pesticide
Drug effect, extend the service life of pesticide.
(2) agricultural chemicals suspension agent of this prevention and treatment prodenia litura provided by the invention uses the aqueous suspension dosage form of environmentally friendly dosage form
Formula, production process without dust pollution, aqueous pesticide is opposite small with effect on environment for pulvis and granule production process, makes
With conveniently.
Specific embodiment
The following is a clear and complete description of the technical scheme in the embodiments of the invention, it is clear that described embodiment
Only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, the common skill in this field
Art personnel all other embodiment obtained without making creative work belongs to the model that the present invention protects
It encloses.
Embodiment 1:
A kind of agricultural chemicals suspension agent for preventing and treating prodenia litura is present embodiments provided, including by mass percentage such as the following group
Point: 1,500,000,000 PIB/g of prodenia litura nuclear polyhedrosis virus, effective cypermethrin 10%, gamma cyhalothrin 10%, benzoic acid
Sodium 0.8%, alkyl phenol polyethenoxy ether sodium sulfate salt 3.0%, polyoxyethylene polyoxypropylene block copolymer 3.0%, xanthan gum
0.2%, ethylene glycol 5.0%, organic silicon defoamer 0.2%, surplus is deionized water.
The preparation method of the agricultural chemicals suspension agent specifically comprises the following steps:
(1) prodenia litura nuclear polyhedrosis virus, pyrethrin pesticide, adjuvant are put into deionized water by design quantity
In, and 1h is sheared in high-speed shearing machine, obtain mixed liquor；
(2) mixed liquor obtained in step (1) is sanded by two sand mill series connection, diameter of particle in mixed liquor is made to exist
5 μm hereinafter, by the suspension being sanded by pneumatic diaphragm be pumped into shearing homogeneous kettle in homogeneous 1h to get the agricultural-chemical suspension
Agent.
Embodiment 2:
A kind of agricultural chemicals suspension agent for preventing and treating prodenia litura is present embodiments provided, including by mass percentage such as the following group
Point: 0.01 hundred million PIB/g of prodenia litura nuclear polyhedrosis virus, effective cypermethrin 10%, gamma cyhalothrin 15%, benzene first
Sour sodium 1.0%, alkyl phenol polyethenoxy ether sodium sulfate salt 1.0%, polyoxyethylene polyoxypropylene block copolymer 5.0%, xanthan
Glue 2%, ethylene glycol 3.0%, organic silicon defoamer 0.1%, surplus are deionized water.
The preparation method of the agricultural chemicals suspension agent is same as Example 1.
Embodiment 3:
A kind of agricultural chemicals suspension agent for preventing and treating prodenia litura is present embodiments provided, including by mass percentage such as the following group
Point: 2,000,000,000 PIB/g of prodenia litura nuclear polyhedrosis virus, gamma cyhalothrin 0.2%, sodium benzoate 0.2%, alkyl phenol
Polyethenoxy ether sodium sulfate salt 5.0%, polyoxyethylene polyoxypropylene block copolymer 1.0%, xanthan gum 0.05%, ethylene glycol
10.0%, organic silicon defoamer 0.5%, surplus is deionized water.
The preparation method of the agricultural chemicals suspension agent is same as Example 1.
Embodiment 4:
A kind of agricultural chemicals suspension agent for preventing and treating prodenia litura is present embodiments provided, including by mass percentage such as the following group
Point: 100,000,000 PIB/g of prodenia litura nuclear polyhedrosis virus, effective cypermethrin 10%, Biphenthrin 5%, sodium benzoate 0.8%,
Polycarboxylate 3.0%, the polyether-modified object 2.0% of modified rosin, xanthan gum 0.2%, propylene glycol 8.0%, organic silicon defoamer
0.2%, surplus is deionized water.
The preparation method of the agricultural chemicals suspension agent is same as Example 1.
Embodiment 5:
A kind of agricultural chemicals suspension agent for preventing and treating prodenia litura is present embodiments provided, including by mass percentage such as the following group
Point: 1,000,000,000 PIB/g of prodenia litura nuclear polyhedrosis virus, effective cypermethrin 0.5%, sodium benzoate 0.8%, polycarboxylate
5.0%, the polyether-modified object 4.0% of modified rosin, xanthan gum 0.05%, propylene glycol 10.0%, organic silicon defoamer 0.1% is remaining
Amount is deionized water.
The preparation method of the agricultural chemicals suspension agent is same as Example 1.
Embodiment 6:
A kind of agricultural chemicals suspension agent for preventing and treating prodenia litura is present embodiments provided, including by mass percentage such as the following group
Point: 0.02 hundred million PIB/g of prodenia litura nuclear polyhedrosis virus, effective cypermethrin 5%, lambda-cyhalothrin 10%, prallethrin
5%, sodium benzoate 0.2%, polycarboxylate 1.0%, the polyether-modified object 0.5% of modified rosin, xanthan gum 2.0%, propylene glycol
3.0%, organic silicon defoamer 0.5%, surplus is deionized water.
The preparation method of the agricultural chemicals suspension agent is same as Example 1.
Embodiment 7:
A kind of agricultural chemicals suspension agent for preventing and treating prodenia litura is present embodiments provided, including by mass percentage such as the following group
Point: 500,000,000 PIB/g of prodenia litura nuclear polyhedrosis virus, gamma cyhalothrin 5%, Biphenthrin 5%, sodium benzoate
0.6%, phosphate 2.0%, polycarboxylate 2.5%, polyoxyethylene polyoxypropylene block copolymer 1.5%, xanthan gum 0.12%,
Glycerol 8.0%, organic silicon defoamer 0.2%, surplus are deionized water.
The preparation method of the agricultural chemicals suspension agent is same as Example 1.
Embodiment 8:
A kind of agricultural chemicals suspension agent for preventing and treating prodenia litura is present embodiments provided, including by mass percentage such as the following group
Point: 500,000,000 PIB/g of prodenia litura nuclear polyhedrosis virus, effective cypermethrin 10%, Fenpropathrin 5%, sodium benzoate 0.8%,
Phosphate 3.0%, polycarboxylate 3.0%, polyoxyethylene polyoxypropylene block copolymer 0.5%, xanthan gum 0.05%, glycerol
10.0%, organic silicon defoamer 0.5%, surplus is deionized water.
The preparation method of the agricultural chemicals suspension agent is same as Example 1.
Embodiment 9:
A kind of agricultural chemicals suspension agent for preventing and treating prodenia litura is present embodiments provided, including by mass percentage such as the following group
Point: 0.02 hundred million PIB/g of prodenia litura nuclear polyhedrosis virus, gamma cyhalothrin 0.5%, sodium benzoate 0.2%, phosphoric acid
Ester 1.0%, polycarboxylate 0.5%, polyoxyethylene polyoxypropylene block copolymer 15%, xanthan gum 2.0%, glycerol 3.0% have
Machine silicon defoaming agent 0.1%, surplus are deionized water.
The preparation method of the agricultural chemicals suspension agent is same as Example 1.
Embodiment 10:
A kind of agricultural chemicals suspension agent for preventing and treating prodenia litura is present embodiments provided, including by mass percentage such as the following group
Point: 0.5 hundred million PIB/g of prodenia litura nuclear polyhedrosis virus, Biphenthrin 8%, alkyl ethylsulfonic acid sodium salt 2.0%, polycarboxylic acids
Salt 3.0%, fatty alcohol polyoxyethylene ether 2.0%, xanthan gum 0.1%, propylene glycol 8.0%, organic silicon defoamer 0.2%, surplus
For deionized water.
The preparation method of the agricultural chemicals suspension agent is same as Example 1.
Embodiment 11:
A kind of agricultural chemicals suspension agent for preventing and treating prodenia litura is present embodiments provided, including by mass percentage such as the following group
Point: 400,000,000 PIB/g of prodenia litura nuclear polyhedrosis virus, gamma cyhalothrin 0.3%, decis 0.5%, alkyl ethyl
Sulfonate sodium 3.0%, polycarboxylate 1.0%, fatty alcohol polyoxyethylene ether 4.0%, xanthan gum 0.05%, propylene glycol 10.0%,
Organic silicon defoamer 0.1%, surplus are deionized water.
The preparation method of the agricultural chemicals suspension agent is same as Example 1.
Embodiment 12:
A kind of agricultural chemicals suspension agent for preventing and treating prodenia litura is present embodiments provided, including by mass percentage such as the following group
Point: 0.02 hundred million PIB/g of prodenia litura nuclear polyhedrosis virus, lambda-cyhalothrin 6%, effective cypermethrin 6%, alkyl ethyl sulphur
Acid sodium-salt 1.0%, polycarboxylate 4.0%, fatty alcohol polyoxyethylene ether 0.5%, xanthan gum 2.0%, propylene glycol 3.0% are organic
Silicon defoaming agent 0.5%, surplus are deionized water.
The preparation method of the agricultural chemicals suspension agent is same as Example 1.
Embodiment 13:
A kind of agricultural chemicals suspension agent for preventing and treating prodenia litura is present embodiments provided, including by mass percentage such as the following group
Point: 100,000,000 PIB/g of prodenia litura nuclear polyhedrosis virus, gamma cyhalothrin 5%, acrylic acid graft copolymer 3.0% gather
Ethylene oxide polyoxypropylene block copolymers 1.5%, xanthan gum 0.15%, ethylene glycol 4.0%, organic silicon defoamer 0.2%, surplus
For deionized water.
The preparation method of the agricultural chemicals suspension agent is same as Example 1.
Embodiment 14:
A kind of agricultural chemicals suspension agent for preventing and treating prodenia litura is present embodiments provided, including by mass percentage such as the following group
Point: 200,000,000 PIB/g of prodenia litura nuclear polyhedrosis virus, Biphenthrin 0.8%, etrafluorine ethofenprox 0.2% are acrylic acid-grafted total
Polymers 4.0%, polyoxyethylene polyoxypropylene block copolymer 0.5%, xanthan gum 0.05%, ethylene glycol 3.0%, organosilicon disappears
Infusion 0.5%, surplus are deionized water.
The preparation method of the agricultural chemicals suspension agent is same as Example 1.
Embodiment 15:
A kind of agricultural chemicals suspension agent for preventing and treating prodenia litura is present embodiments provided, including by mass percentage such as the following group
Point: 0.02 hundred million PIB/g of prodenia litura nuclear polyhedrosis virus, effective cypermethrin 5%, silafluofene 5% are acrylic acid-grafted total
Polymers 1.0%, polyoxyethylene polyoxypropylene block copolymer 4.0%, xanthan gum 2.0%, ethylene glycol 10.0%, organosilicon defoaming
Agent 0.1%, surplus are deionized water.
The preparation method of the agricultural chemicals suspension agent is same as Example 1.
Embodiment 16:
The present embodiment has investigated the gamma cyhalothrin, effective cypermethrin, Biphenthrin, prodenia litura of different ratio
Nuclear polyhedrosis virus carries out co-toxicity testing experiment to prodenia litura, and detailed process is as follows:
1. instrument: assay balance (a ten thousandth), illumination box, liquid-transfering gun, graduated cylinder, tweezers, marking pen, beaker, appearance
Measuring bottle, culture dish, raising bottle etc..
2. equipment: constant temperature insectary.
3. experimental condition and material
3.1 biological test materials
3 instar larvae of prodenia litura, Wuhan Chu Qiang Biotechnology Co., Ltd provide, and select age consistent healthy 3 age children
Worm carries out toxicity test.Rearing conditions: temperature is (26 ± 2 DEG C), illumination 14h/d, humidity 85%.
3.2 test medicine
10000000000 PIB/g prodenia litura nuclear polyhedrosis virus raw medicines and 97% gamma cyhalothrin, 98% efficient chlorine cyanogen
Pyrethroids raw medicine, 97% Biphenthrin.
4. test procedure is using leaf dipping method measurement drug combination virulence activity
4.1 test materials prepare
Fresh cabbage leaves are selected, cleans, dries in the air to surface in shady place without washmarking, it is spare.
The configuration of 4.2 medical fluids
Prodenia litura nuclear polyhedrosis virus raw medicine is diluted to 10,000,000 with the sterile solution of 2% methylcellulose
The mother liquor of PIB/g.
97% gamma cyhalothrin, 98% effective cypermethrin raw medicine, 97% Biphenthrin are used into a small amount of acetone respectively
After dissolution, then with containing 0.1%T-80 aqueous solution dilute, be made into 1% mother liquor.Four single doses are made into such as table 1 with sterile water
Shown in the mixture mother liquor of different ratio A, B, C, D, E, F, G7 processing are diluted with sterile water according to pre-made experimental result
For 5 mass concentration gradients: 20,40,80,160,320mg/L measure compound drug co-toxicity according to leaf dipping method.
Table 1: pyrethroids class raw medicine and the processing of the proportion of prodenia litura nuclear polyhedrosis virus
4.3 chemicals treatment
Fresh cabbage leaves are put into the medical fluid of each concentration for the treatment of and soak 30s, dried after taking-up, encased with the absorbent cotton soaked
Hypophyll is put into glass (culture cup), every glass of 6 leaves, accesses 3 instar larvae of prodenia litura 20, and every concentration is repeated 3 times, altogether
60, notice that the humidity of the absorbent cotton of protection package dish leaf, anti-dish leaf are withered after processing.
4.4 raisings and observation
Prodenia litura test worm that treated is placed in the intelligent illumination box of (26 ± 1 DEG C), periodicity of illumination L:D=16:8
Middle raising and observation.
4.5 checking
Checkout facility after 48h is as a result, touch polypide with writing brush, it is that death standard compares the death rate that larva, which is unable to the coordinated movement of various economic factors,
It is efficiency test less than 10%.
5 data statistics and analysis
5.1 calculation method
According to survey data, the corrected mortality of each processing is calculated；It is calculated by formula (1), (2), calculated result retains
To 2 significant digits:
The dead total borer population of borer population/processing (1) of the death rate (%)=100*,
Corrected mortality (%)=* 100 (2) of (the processing death rate-control death rate)/(1- compares the death rate)；
Co-toxicity coefficient measuring method is carried out by Sun Yunpei (1960) method, and the specific method is as follows:
Using A as standard agent, then the theoretical toxicity index of A is 100,
Theoretical toxicity index (TI)=(LC of A medicament of B50The LC of ÷ B medicament50) * 100,
(A+B) actual toxicity index (ATI)=(LC of A medicament of mixture50The LC of ÷ mixture50) * 100,
(A+B) theoretical toxicity index (TTI)=A medicament TI*A medicament of mixture accounts for the TI*B medicine of mixture %+B medicament
Agent accounts for mixture %,
(A+B) co-toxicity coefficient of mixture=(ATI ÷ TTI) * 100；
Co-toxicity coefficient indicates synergistic effect when being greater than 120, and summation action is indicated between 80~120, is when less than 80
Antagonism.
5.2 statistical analysis
Test data is all made of DPS and carries out processing analysis, calculate the virulence regression equation of each medicament, the lethal concentration of 50,
The results are shown in Table 2 for coefficient R.
Table 2: each reagent treatment statisticallys analyze 3 instar larvae life Activity Results of prodenia litura
From table 2 it can be seen that 1% gamma cyhalothrin, 1% effective cypermethrin, 1% Biphenthrin are respectively with 0.1
Hundred million PIB/g prodenia litura nuclear polyhedrosis virus are compounded, three kind pyrethroids classes originals different to prodenia litura virulence by different ratio
Co-toxicity coefficient after medicine is individually compounded with prodenia litura nuclear polyhedrosis virus by 2:1 is all larger than 120, shows as synergy；
The co-toxicity coefficient that processing two kinds of pyrethroids class raw medicines of H, I, J are compounded with prodenia litura nuclear polyhedrosis virus table between 80~120
It is now that summation action, wherein effective cypermethrin and prodenia litura nuclear polyhedrosis virus press 2:1 co-toxicity coefficient highest, synergy effect
Fruit is best.
Embodiment 17:
0.1 hundred million PIB/g prodenia litura nuclear polyhedrosis virus, 2% effective cypermethrin suspending agent is used for by the present embodiment
The field trial of prodenia litura, detailed process is as follows:
1, crop and object: wild cabbage prodenia litura are tested.
2, experimental pharmacy: the efficient chlorine cyanogen of 0.1 hundred million PIB/g prodenia litura nuclear polyhedrosis virus 2% in embodiment 11
Pyrethroids suspending agent (Wuhan Chu Qiang Biotechnology Co., Ltd), 10% effective cypermethrin suspending agent (Wuhan Chu Qiang biotechnology
Co., Ltd), 100,000,000 PIB/g prodenia litura nuclear polyhedrosis virus are (by 2% first of prodenia litura nuclear polyhedrosis virus raw medicine
The sterile solution of base cellulose is diluted to the mother liquor of 100,000,000 PIB/g), it is specific as shown in table 3.
3, experimental design: 6 processing, 4 repetitions of every processing are arranged in experimental pharmacy altogether, and cell processing uses random alignment,
20 ㎡ of plot area, application method be it is spraying, start to be administered in wild cabbage prodenia litura early period of origination, entire breeding time is administered 2 times,
Application interval 7~10 days.
4, investigation and statistical method: counting the work larva number that every cell is no less than different larval instar on 10 plants of wild cabbages, investigates whole
A plant；Radix investigation is carried out before treatment, is carried out second within 1~3 day after processing and is investigated, handles 7~14 days progress thirds
Secondary investigation.
5, drug effect calculation method
Revision insect recluced rate (%)=[borer population living before (borer population living after borer population-medicine of living before medicine)/medicine] × 100%；
Preventive effect (%)=[(processing group Revision insect recluced rate-control group Revision insect recluced rate)/(100- control group insect population decline
Rate)] × 100%.
6, investigation result is as shown in table 4.
Table 3: experimental pharmacy design
Table 4: to the field test results of prodenia litura
As shown in Table 4,1 day after medicine, processing 1~3 that effective cypermethrin is compounded with prodenia litura nuclear polyhedrosis virus
Preventive effect be 77.85%~91.61%, hence it is evident that higher than control single dose processing 4 66.47% and processing 5 21.93%, performance
Preferable quick-acting out.3 days after medicine, preventive effect increases, wherein effective cypermethrin and prodenia litura nucleopolyhedrosis
The preventive effect of poison compounding is 81.56%~91.98%, higher than the preventive effect of control single dose；7 days after medicine, effective cypermethrin and twill
The preventive effect of californica nuclear polyhedrosis virus compounding is 83.61%~92.84%, and the preventive effect of 10% effective cypermethrin is
72.92%, the preventive effect of 100,000,000 prodenia litura nuclear polyhedrosis virus is 71.92%, and the preventive effect of compounding is apparently higher than single dose；And
During application, phytotoxicity is not found, to crop safety.Therefore, effective cypermethrin and prodenia litura nuclear polyhedrosis virus are pressed
Ratio compounding has apparent synergistic effect, quick-acting and preventive effect not only can be improved, but also to crop safety.
The foregoing examples are only illustrative of the present invention, does not constitute the limitation to protection scope of the present invention, all
It is within being all belonged to the scope of protection of the present invention with the same or similar design of the present invention.
Claims (10)
1. a kind of agricultural chemicals suspension agent for preventing and treating prodenia litura, it is characterised in that: including following component by mass percentage: tiltedly
Autographa spp nuclear polyhedrosis virus 0.01~3,000,000,000 PIB/g, pyrethrin pesticide 0.1~30%, adjuvant 2.5~28.5% are remaining
Amount is deionized water.
2. the agricultural chemicals suspension agent of prevention and treatment prodenia litura as described in claim 1, it is characterised in that: the pyrethrin pesticide includes
Lambda-cyhalothrin, gamma cyhalothrin, cypermethrin, effective cypermethrin, Fenpropathrin, Biphenthrin, decis, fluorine
At least one of silicon pyrethroids, prallethrin and etrafluorine ethofenprox.
3. the agricultural chemicals suspension agent of prevention and treatment prodenia litura as described in claim 1, it is characterised in that: the pyrethrin pesticide includes
At least one of gamma cyhalothrin, effective cypermethrin, Biphenthrin.
4. the agricultural chemicals suspension agent of prevention and treatment prodenia litura as described in claim 1, it is characterised in that: the adjuvant includes dispersion
Agent, wetting agent, thickener, antifreezing agent, defoaming agent and preservative, and each component accounts for the degree of agricultural chemicals suspension agent gross mass
As follows: dispersing agent 1.0~8.0%, wetting agent 0.5~8.0%, thickener 0.05~2.0%, antifreezing agent 1.0~10.0% disappear
Infusion 0.1~0.5%, preservative 0.1~2.0%.
5. the agricultural chemicals suspension agent of prevention and treatment prodenia litura as claimed in claim 4, it is characterised in that: including by mass percentage
Following component: prodenia litura nuclear polyhedrosis virus 0.01~2,000,000,000 PIB/g, pyrethrin pesticide 0.2~25%, sodium benzoate
0.2~1.0%, alkyl phenol polyethenoxy ether sodium sulfate salt 1.0~5.0%, polyoxyethylene polyoxypropylene block copolymer 1.0~
5.0%, xanthan gum 0.05~2.0%, ethylene glycol 3.0~10.0%, organic silicon defoamer 0.1~0.5%, surplus is deionization
Water.
6. the agricultural chemicals suspension agent of prevention and treatment prodenia litura as claimed in claim 4, it is characterised in that: including by mass percentage
Following component: prodenia litura nuclear polyhedrosis virus 0.02~1,000,000,000 PIB/g, pyrethrin pesticide 0.5~20%, sodium benzoate
0.2~0.8%, polycarboxylate 1.0~5.0%, the polyether-modified object 0.5~4.0% of modified rosin, xanthan gum 0.05~2.0%,
Propylene glycol 3.0~10.0%, organic silicon defoamer 0.1~0.5%, surplus are deionized water.
7. the agricultural chemicals suspension agent of prevention and treatment prodenia litura as claimed in claim 4, it is characterised in that: including by mass percentage
Following component: prodenia litura nuclear polyhedrosis virus 0.02~500,000,000 PIB/g, pyrethrin pesticide 0.5~15%, sodium benzoate
0.2~0.8%, phosphate 1.0~3.0%, polycarboxylate 0.5~3.0%, polyoxyethylene polyoxypropylene block copolymer 0.5
~4.0%, xanthan gum 0.05~2.0%, glycerol 3.0~10.0%, organic silicon defoamer 0.1~0.5%, surplus is deionization
Water.
8. the agricultural chemicals suspension agent of prevention and treatment prodenia litura as claimed in claim 4, it is characterised in that: including by mass percentage
Following component: prodenia litura nuclear polyhedrosis virus 0.02~400,000,000 PIB/g, pyrethrin pesticide 0.8~12%, alkyl ethyl
Sulfonate sodium 1.0~3.0%, polycarboxylate 1.0~4.0%, fatty alcohol polyoxyethylene ether 0.5~4.0%, xanthan gum 0.05~
2.0%, propylene glycol 3.0~10.0%, organic silicon defoamer 0.1~0.5%, surplus is deionized water.
9. the agricultural chemicals suspension agent of prevention and treatment prodenia litura as claimed in claim 4, it is characterised in that: including by mass percentage
Following component: prodenia litura nuclear polyhedrosis virus 0.02~200,000,000 PIB/g, pyrethrin pesticide 1.0~10%, acrylic acid connects
Graft copolymer 1.0~4.0%, polyoxyethylene polyoxypropylene block copolymer 0.5~4.0%, xanthan gum 0.05~2.0%, second
Glycol 3.0~10.0%, organic silicon defoamer 0.1~0.5%, surplus are deionized water.
10. the preparation method of the agricultural chemicals suspension agent of prevention and treatment prodenia litura as described in any one of claims 1 to 9, feature exist
In: include the following steps:
1) prodenia litura nuclear polyhedrosis virus, pyrethrin pesticide, adjuvant are put into deionized water by design quantity, and
1h is sheared in high-speed shearing machine, obtains mixed liquor；
2) mixed liquor obtained in step 1) is sanded by the series connection of two sand mills, make in mixed liquor diameter of particle 5 μm with
Under, by the suspension being sanded by pneumatic diaphragm be pumped into shearing homogeneous kettle in homogeneous 1h to get the agricultural chemicals suspension agent.
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