CN109996877A - 一种用于核酸样品标识的基因标签、试剂盒及其应用 - Google Patents

一种用于核酸样品标识的基因标签、试剂盒及其应用 Download PDF

Info

Publication number
CN109996877A
CN109996877A CN201680091177.4A CN201680091177A CN109996877A CN 109996877 A CN109996877 A CN 109996877A CN 201680091177 A CN201680091177 A CN 201680091177A CN 109996877 A CN109996877 A CN 109996877A
Authority
CN
China
Prior art keywords
nucleic acid
gene label
gene
application
sequencing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201680091177.4A
Other languages
English (en)
Chinese (zh)
Inventor
张东
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BGI Shenzhen Co Ltd
BGI Genomics Co Ltd
Original Assignee
BGI Shenzhen Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BGI Shenzhen Co Ltd filed Critical BGI Shenzhen Co Ltd
Publication of CN109996877A publication Critical patent/CN109996877A/zh
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201680091177.4A 2016-12-16 2016-12-16 一种用于核酸样品标识的基因标签、试剂盒及其应用 Pending CN109996877A (zh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2016/110457 WO2018107481A1 (fr) 2016-12-16 2016-12-16 Étiquette génétique pour identification d'échantillon d'acide nucléique, kit et application de celle-ci

Publications (1)

Publication Number Publication Date
CN109996877A true CN109996877A (zh) 2019-07-09

Family

ID=62557880

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201680091177.4A Pending CN109996877A (zh) 2016-12-16 2016-12-16 一种用于核酸样品标识的基因标签、试剂盒及其应用

Country Status (2)

Country Link
CN (1) CN109996877A (fr)
WO (1) WO2018107481A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110656157B (zh) * 2019-10-16 2023-09-08 重庆市人口和计划生育科学技术研究院 用于高通量测序样本溯源的质控品及其设计和使用方法
CN111304309A (zh) * 2020-03-06 2020-06-19 上海韦翰斯生物医药科技有限公司 一种测序平台标签序列污染的检测方法
CN112251501B (zh) * 2020-10-28 2023-07-25 深圳人体密码基因科技有限公司 一种内参基因集合及其筛选方法、通用引物组、试剂盒、反应体系及应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101720359A (zh) * 2007-06-01 2010-06-02 454生命科学公司 从多重混合物中识别个别样本的系统和方法
CN102653784A (zh) * 2011-03-03 2012-09-05 深圳华大基因科技有限公司 用于多重核酸测序的标签及其使用方法
CN105349617A (zh) * 2014-08-19 2016-02-24 复旦大学 一种对高通量rna测序数据的质量控制方法及装置

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101720359A (zh) * 2007-06-01 2010-06-02 454生命科学公司 从多重混合物中识别个别样本的系统和方法
CN102653784A (zh) * 2011-03-03 2012-09-05 深圳华大基因科技有限公司 用于多重核酸测序的标签及其使用方法
CN105349617A (zh) * 2014-08-19 2016-02-24 复旦大学 一种对高通量rna测序数据的质量控制方法及装置

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
GLENN K. FU ET AL.: "Molecular indexing enables quantitative targeted RNA sequencing and reveals poor efficiencies in standard library preparations", 《PNAS》 *
黄欢 等: "一种无染料基于序列标签结合焦测序解码的多基因表达量同时测定技术及在大肠癌诊断中的应用", 《分析化学》 *

Also Published As

Publication number Publication date
WO2018107481A1 (fr) 2018-06-21

Similar Documents

Publication Publication Date Title
Krijger et al. 4C-seq from beginning to end: A detailed protocol for sample preparation and data analysis
CN106367485B (zh) 一种用于检测基因突变的多定位双标签接头组及其制备方法和应用
CN107002292B (zh) 一种核酸的双接头单链环状文库的构建方法和试剂
Sun et al. High-resolution enzymatic mapping of genomic 5-hydroxymethylcytosine in mouse embryonic stem cells
US9334532B2 (en) Complexity reduction method
US8367334B2 (en) Methods, systems and kits for detecting protein-nucleic acid interactions
US20150199474A1 (en) Methods and systems for aligning sequences in the presence of repeating elements
CN102409047B (zh) 一种构建杂交测序文库的方法
CN110129415B (zh) 一种ngs建库分子接头及其制备方法和用途
US20060281082A1 (en) Genome partitioning
US11597968B2 (en) Ribonucleic acid (RNA) interactions
CN105754995A (zh) 构建待测基因组的dna测序文库的方法及其应用
US20210262034A1 (en) Methods for identifying and using small rna predictors
CN109996877A (zh) 一种用于核酸样品标识的基因标签、试剂盒及其应用
EP3674413A1 (fr) Sonde et procédé destinés à une région cible de capture ciblée par le séquençage à haut débit utilisés pour la détection de mutations de gène ainsi que de types de fusion de gène connus et non connus
AU2016321204B2 (en) Genetic copy number determination using high throughput multiplex sequencing of smashed nucleotides
CN110656157B (zh) 用于高通量测序样本溯源的质控品及其设计和使用方法
WO2018009702A1 (fr) Marqueurs de méthylation dans le traitement de la leucémie et leurs utilisations
CN107109698A (zh) Rna stitch测序:用于直接映射细胞中rna:rna相互作用的测定
WO2017066907A1 (fr) Procédé de construction d'une bibliothèque de hi-c ayant un rapport de données utiles élevé
CN109762915A (zh) 一种细菌耐药基因的检测方法及其专用试剂盒
CN110970091B (zh) 标签质控的方法及装置
CN105803054A (zh) 试剂盒及其在检测唇腭裂相关基因中的用途
CN113046835A (zh) 检测慢病毒插入位点的测序文库构建方法和慢病毒插入位点检测方法
CN113811620A (zh) 巢式多重pcr高通量测序文库制备方法及试剂盒

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 40010182

Country of ref document: HK

RJ01 Rejection of invention patent application after publication

Application publication date: 20190709

RJ01 Rejection of invention patent application after publication