CN109991063A - The paraffin section method of Paris polyphylla root tuber - Google Patents
The paraffin section method of Paris polyphylla root tuber Download PDFInfo
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- CN109991063A CN109991063A CN201910338345.0A CN201910338345A CN109991063A CN 109991063 A CN109991063 A CN 109991063A CN 201910338345 A CN201910338345 A CN 201910338345A CN 109991063 A CN109991063 A CN 109991063A
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- Prior art keywords
- paris polyphylla
- root tuber
- polyphylla root
- wax
- sample
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- 241000244987 Daiswa polyphylla Species 0.000 title claims abstract description 132
- 238000000034 method Methods 0.000 title claims abstract description 53
- 239000012188 paraffin wax Substances 0.000 title claims abstract description 50
- 230000018044 dehydration Effects 0.000 claims abstract description 29
- 238000006297 dehydration reaction Methods 0.000 claims abstract description 29
- 230000005855 radiation Effects 0.000 claims abstract description 21
- 238000004043 dyeing Methods 0.000 claims abstract description 17
- 238000012545 processing Methods 0.000 claims abstract description 6
- 239000001993 wax Substances 0.000 claims description 59
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 54
- 239000003795 chemical substances by application Substances 0.000 claims description 24
- 239000007788 liquid Substances 0.000 claims description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 210000002583 cell-derived microparticle Anatomy 0.000 claims description 14
- 229960004756 ethanol Drugs 0.000 claims description 14
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 12
- 239000003708 ampul Substances 0.000 claims description 8
- 229960000935 dehydrated alcohol Drugs 0.000 claims description 7
- 239000012153 distilled water Substances 0.000 claims description 7
- 238000002360 preparation method Methods 0.000 claims description 7
- 230000008021 deposition Effects 0.000 claims description 6
- 235000011187 glycerol Nutrition 0.000 claims description 6
- 238000009833 condensation Methods 0.000 claims description 5
- 230000005494 condensation Effects 0.000 claims description 5
- 239000004677 Nylon Substances 0.000 claims description 4
- 239000007864 aqueous solution Substances 0.000 claims description 4
- 238000005138 cryopreservation Methods 0.000 claims description 4
- 238000004090 dissolution Methods 0.000 claims description 4
- 229940057995 liquid paraffin Drugs 0.000 claims description 4
- 229920001778 nylon Polymers 0.000 claims description 4
- RZSYLLSAWYUBPE-UHFFFAOYSA-L Fast green FCF Chemical compound [Na+].[Na+].C=1C=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C(=CC(O)=CC=2)S([O-])(=O)=O)C=CC=1N(CC)CC1=CC=CC(S([O-])(=O)=O)=C1 RZSYLLSAWYUBPE-UHFFFAOYSA-L 0.000 claims description 3
- 230000004308 accommodation Effects 0.000 claims description 3
- 210000000232 gallbladder Anatomy 0.000 claims description 3
- 238000001727 in vivo Methods 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 15
- 230000008520 organization Effects 0.000 abstract description 6
- 235000019441 ethanol Nutrition 0.000 description 21
- 210000004027 cell Anatomy 0.000 description 12
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 8
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 8
- 239000000463 material Substances 0.000 description 5
- 238000005086 pumping Methods 0.000 description 5
- 241000209094 Oryza Species 0.000 description 4
- 235000007164 Oryza sativa Nutrition 0.000 description 4
- 229960000583 acetic acid Drugs 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 4
- 239000000975 dye Substances 0.000 description 4
- 239000012362 glacial acetic acid Substances 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 235000009566 rice Nutrition 0.000 description 4
- 206010010904 Convulsion Diseases 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000036461 convulsion Effects 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000002356 single layer Substances 0.000 description 2
- 206010007247 Carbuncle Diseases 0.000 description 1
- 241000234280 Liliaceae Species 0.000 description 1
- 208000004078 Snake Bites Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 206010000269 abscess Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000003211 malignant effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000009306 yunnan baiyao Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/42—Low-temperature sample treatment, e.g. cryofixation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/44—Sample treatment involving radiation, e.g. heat
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
- G01N2001/2873—Cutting or cleaving
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/305—Fixative compositions
Abstract
The invention discloses a kind of paraffin section methods of Paris polyphylla root tuber, wherein includes: that Paris polyphylla root tuber sample is successively obtained the paraffin section of the Paris polyphylla root tuber after fixation, dehydration, transparent, waxdip, embedding, slice, exhibition piece, dewaxing, dyeing and mounting；Wherein, the method for the fixation are as follows: be placed in Paris polyphylla root tuber sample in the environment of fixer, negative pressure and infrared radiation 24 hours or more.Processing is fixed to Paris polyphylla root tuber using the mode of infrared radiation combination negative pressure in the present invention, infrared radiation enables to Paris polyphylla root tuber tissue to become active, in conjunction with the effect of negative pressure, the gas of organization internal is run out of in time, fixer can more quickly enter Paris polyphylla root tuber organization internal and complete cyto-architectural fixation, improve fixed effect and efficiency.
Description
Technical field
The present invention relates to paraffin section technical fields, it is more particularly related to which a kind of paraffin of Paris polyphylla root tuber is cut
Piece method.
Background technique
Paris polyphylla Paris polyphylla Smith is Liliaceae Liliaceae Paris Paris herbaceos perennial,
It is moist to be common in 1400-3100 meters of height above sea level of shrubbery or evergreen broad-leaved hayashishita in Yunnan, Guangxi, Sichuan, Guizhou and other places for main product
Ground.It is used as medicine with dry rhizome, there is the effect of clearing heat and detoxicating, swelling and pain relieving, cool liver arresting convulsion, for malignant boil swollen carbuncle swells, abscess of throat, poison
The diseases such as snake bite, the pain of injury caused by falling and tumbling, convulsion are Yunnan Baiyao, the important original for taking the Chinese patent drugs and preparation such as life pellet, Gong Xue Ning by force
Material.The version Pharmacopoeia of the People's Republic of China in 2015 records this kind [2].Since the regeneration of Paris polyphylla wild resource is slower, for a long time
It is excessively excavated by people, and its seed has the physiological property of secondary development, natural propagation rate is low, so that wild resource is on the verge of
It is exhausted.But since the market demand is aggravated in recent years, in addition the update of wild resource is slow, the Paris polyphylla market price rises year by year, certified products
Medicinal material in the phenomenon that often mixing the spurious with the genuine, therefore, the microscopic features to Paris polyphylla medicinal material is often needed to identify, separated true
Puppet, the market of specification Paris polyphylla medicinal material.
It carries out microscopic features identification and needs for Paris polyphylla medicinal material to be made into slice, conventional paraffin method is related to fixed, de-
The techniques such as water, transparent, waxdip, embedding, slice, exhibition piece, roasting piece, dewaxing, rehydration, dyeing, mounting, though it is simple in the process,
Paris polyphylla root tuber quality is harder, air etc. is contained in internal space between cells causes fixer in fixing step to be not easily accessible；Meanwhile solid
It needs to be evacuated in fixed step so that the air in space between cells is run out of inside Paris polyphylla root tuber, traditional air-exhaust method is using artificial
Operating syringe is evacuated repeatedly, if on the one hand the set time is longer, manual operation syringe extremely consumption manpower and efficiency compared with
It is low；The method pumping effect of another aspect syringe pumping is not good enough, and the air in space between cells is run out of not enough inside building root block
Thoroughly.These problems cause the effect for manufacturing the more time-consuming and last slice of Paris polyphylla root tuber paraffin section bad.
Therefore, it needs to design a kind of stone that fixer can be promoted to enter cell and the pumping efficiency in raising fixing step
Wax dicing method.
Summary of the invention
It is an object of the invention to solve at least the above defect, and provide the advantages of at least will be described later.
It is a further object to provide a kind of Paris polyphylla root tubers that fixer can be promoted to enter Paris polyphylla root tuber cell
Paraffin section method.
In order to realize these purposes and other advantages according to the present invention, the paraffin that the present invention provides a kind of Paris polyphylla root tuber is cut
Piece method, wherein include:
By Paris polyphylla root tuber sample successively by fixation, dehydration, transparent, waxdip, embedding, slice, open up piece, dewaxing, dyeing and
The paraffin section of the Paris polyphylla root tuber is obtained after mounting；
Wherein, the method for the fixation are as follows: Paris polyphylla root tuber sample is placed in the environment of fixer, negative pressure and infrared radiation
In 24 hours or more.
Infrared radiation enables to Paris polyphylla root tuber tissue to become active, in conjunction with the effect of negative pressure, the gas of organization internal and
When run out of, fixer can more quickly enter Paris polyphylla root tuber organization internal and complete cyto-architectural fixation, therefore negative pressure
Mode in conjunction with infrared radiation promotes fixer and enters Paris polyphylla root tuber cell.
Preferably, in the paraffin section method of the Paris polyphylla root tuber, the fixer is FAA fixer, the FAA
Glycerine is mixed in fixer；
The negative pressure is -0.01~-0.09Mpa: the length, width and height of the Paris polyphylla root tuber sample are 5 centimetres；
The infrared radiation is the Infrared irradiation of 600~1000nm of wavelength.The infrared light of this wavelength is conducive to by Paris polyphylla block
Root absorbs, and Paris polyphylla root tuber temperature can be made to increase, so that Paris polyphylla tissue becomes active.
Preferably, in the paraffin section method of the Paris polyphylla root tuber, the negative pressure is in following mechanical periodicity:
It is kept for 5 minutes under -0.01Mpa；It is kept for 15 minutes under -0.03Mpa；It is kept for 20 minutes under -0.05Mpa；-
It is kept for 15 minutes under 0.07Mpa；It is kept for 5 minutes under -0.09Mpa, so as to form a cycle and is recycled until fixation terminates.
Negative pressure rises in gradient can promote the gas inside Paris polyphylla root tuber to run out of, while reduce the volatilization of fixer, and
And after negative pressure cyclically-varying runs out of gas, fixer replacement gas enters Paris polyphylla root tuber cell interior, to improve
Paris polyphylla root tuber fixed effect and efficiency.
Preferably, in the paraffin section method of the Paris polyphylla root tuber, the step of the dehydration are as follows: by Paris polyphylla root tuber sample
According to 50%, 70%, 85%, 95% concentration of alcohol, respectively dehydration is primary step by step in cryopreservation tube for product, and 1.5~2h, places into every time
95% ethanol dehydration 1h, last 100% ethanol dehydration twice, each each 30~45min.
Preferably, in the paraffin section method of the Paris polyphylla root tuber, the transparent step are as follows: first use 1/2 nothing
+ 1/2 environment friendly transparent agent of water-ethanol handles twice Paris polyphylla root tuber sample clear；
Paris polyphylla root tuber sample clear is handled twice with environment friendly transparent agent again；
The time of each transparent processing is 1~2 hour.
Preferably, in the paraffin section method of the Paris polyphylla root tuber, the step of the waxdip are as follows:
Paris polyphylla root tuber sample is put into ampoule bottle, the dipped Paris polyphylla root tuber sample of environment friendly transparent agent is added, by environment friendly transparent
Agent: rubble wax is added in the mass ratio of rubble wax=1:0.3, after rubble wax is completely dissolved, then it is added 2 by same mass ratio~
Then ampoule bottle is put into 37 DEG C of baking ovens 6~12 hours by 3 rubble waxes；
Then oven temperature is increased to 42 DEG C, divides 3-4 addition rubble wax, until saturation；
Oven temperature is increased to 60 DEG C again, then small by holding 6~12 after same mass ratio 3-4 rubble wax of addition
When；
Then Paris polyphylla root tuber sample is transferred in pure liquid paraffin, after being kept for 4 hours, subsequent embedding can be carried out；
Wherein, the rubble wax the preparation method comprises the following steps: by new wax and using old wax more than three times according to the quality of 1:1
It than mixing, is subsequently placed in 85 DEG C of baking oven and sufficiently dissolves, be cooled and shaped after the nylon net filter using 300 mesh, then cut broken obtain
To the rubble wax.
Preferably, in the paraffin section method of the Paris polyphylla root tuber, the step of the embedding are as follows:
By the rubble wax, sufficiently dissolution obtains liquid wax in 85 DEG C of baking oven, Paris polyphylla root tuber sample is put into described
It in liquid wax, after liquid wax surface condensation, is immediately placed in pre-prepd ice cold water, wax is promoted to be rapidly solidificated into type.
Preferably, in the paraffin section method of the Paris polyphylla root tuber, obtained with a thickness of 10 using microtome~
14 microns of thin slice；
It dewaxes again to the thin slice, the step of the dewaxing are as follows: environment friendly transparent agent 10min；1/2 environment friendly transparent agent and
1/2 pure dehydrated alcohol 3min；100%, 95%, 85%, 70%, 50% alcohol and distilled water each 5~10 minutes.
Preferably, in the paraffin section method of the Paris polyphylla root tuber, the step of the dyeing are as follows:
Thin slice after dewaxing is put in 15~20h of dyeing, distilled water in 0.5%-1% sarranine aqueous solution and washes away extra dye
Liquid, passes through 30%, 50%, 70%, 80%, 95% dehydration of alcohol respectively, every grade 5 minutes, then with containing 0.1% 95% fast green wine
Essence is redyed 10~40 seconds.
Preferably, in the paraffin section method of the Paris polyphylla root tuber, the fixation is carried out using special fixing device
Step, the special fixing device include:
Ectosome, internal to have accommodation space, the upper end closed of the ectosome is simultaneously reserved with insert port in center；
Liner, from the insert port vertically to it is described it is outer in vivo, insulating space, institute are formed between the liner and ectosome
State the space having in liner for accommodating fixer, the liner upper end opening；
Lid comprising cover board and vertical part, the vertical part are vertically connected to the cover board, and the cover cap is bonded to described outer
When body upper end, the vertical part is extend into the liner, and the cover board closes the upper end opening of the liner, wherein described perpendicular
The lower end in portion is provided with the basket for placing Paris polyphylla root tuber sample, and the side wall of the basket is provided with mesh and makes inside and outside basket
Connection；Sample, which is loaded, using basket has the benefit that sample is placed on ratio inside basket compared to using the fixed sample of method of clamping
Relatively flexibly, each face of sample can bear negative pressure and the gas of sample interior is run out of rapidly, and the mode clamped will lead to
The position clamped on sample unbearable negative pressure by shielding.
Aspiration pump, the upper portion side wall of the liner is connected to by tracheae, and the tracheae is in U in the insulating space
Type setting, the U-shaped bottom of the tracheae form liquid deposition portion, and the liquid deposition portion is provided with drain pipe and stretches to outer external
Portion is provided with valve body on the drain pipe；Under negative pressure state, how much fixer, which has, a little volatilizees, and the fixer of volatilization
When by U-shaped tracheae, regelation and be deposited in U-shaped tracheae, and then collected again, reduce the volatilization damage of fixer
It loses.In order to reinforce the condensation effect of the fixer to have volatilized, condenser can be provided in insulating space to be auxiliarily fixed
Liquid condensing.
Infrared lamp is arranged in the insulating space and towards the liner；
Control module comprising for measuring the baroceptor of liner air pressure inside, for incuding liner internal temperature
Temperature sensor, controller；The controller is connect with baroceptor, temperature sensor, aspiration pump and infrared lamp, institute
Controller aspiration pump according to the pressure control of baroceptor is stated, institute is controlled according to the temperature signal of the temperature sensor
State infrared lamp.
Pumping manually is completed using syringe in traditional mode, the low efficiency of pumping, process is uncontrollable, leads to fixation
Efficiency and effect are not good enough.Therefore applicant designs the fixed operation that special special fixing device carries out Paris polyphylla root tuber, wherein
It after Paris polyphylla sample is put into the basket of lid vertical part, is inserted into liner, lid simultaneously closes liner, at this point, controller
The control of internal courage pressure is completed according to the signal of baroceptor, as kept for 5 minutes under -0.01Mpa；It is protected under -0.03Mpa
It holds 15 minutes；It is kept for 20 minutes under -0.05Mpa；It is kept for 15 minutes under -0.07Mpa；It is kept for 5 minutes under -0.09Mpa, with this shape
At a cycle；Controller controls the operation of infrared lamp according to the signal of temperature sensor, interior to prevent infrared lamp irradiation from causing
Gallbladder temperature is excessively high.
Such air pressure and temperature automatic control reduce manpower consumption, improve fixed effect and efficiency.
The present invention is include at least the following beneficial effects:
Processing is fixed to Paris polyphylla root tuber using the mode of infrared radiation combination negative pressure in the present invention, and infrared radiation can make
It obtains Paris polyphylla root tuber tissue to become active, in conjunction with the effect of negative pressure, the gas of organization internal is run out of in time, and fixer can more be accelerated
The Paris polyphylla root tuber organization internal that enters of speed completes cyto-architectural fixation, therefore mode of the negative pressure in conjunction with infrared radiation promotees
Enter Paris polyphylla root tuber cell into fixer.
In fixing step of the invention, negative pressure rises in gradient can promote the gas inside Paris polyphylla root tuber to run out of, simultaneously
The volatilization of fixer is reduced, and after negative pressure cyclically-varying runs out of gas, fixer replacement gas enters Paris polyphylla block
Inside root cells, to improve Paris polyphylla root tuber fixed effect and efficiency.
The present invention devises dedicated fixed device and Paris polyphylla root tuber sample is fixed, and substitutes traditional artificial needle tubing and takes out
The method of gas, reduces manpower consumption, improves fixed effect and efficiency.
The paraffin section of Paris polyphylla root tuber made from the method for the present invention is cut compared to paraffin made from conventional paraffin section method
Piece cell is more complete, and profile is more clear, and effect is more preferable.
Further advantage, target and feature of the invention will be partially reflected by the following instructions, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of special fixing device of the present invention；
Fig. 2 is the frame diagram of control module of the present invention；
Fig. 3 is the electron microscope picture of comparative example 1 of the present invention；
Fig. 4 is the electron microscope picture of embodiment 5 of the present invention.
Specific embodiment
Present invention will be described in further detail below with reference to the accompanying drawings, to enable those skilled in the art referring to specification text
Word can be implemented accordingly.
As illustrated in fig. 1 and 2, the dedicated fixed dress in the fixing step in a kind of paraffin section method for Paris polyphylla root tuber
It sets and includes:
Ectosome 1, internal to have accommodation space, the upper end closed of the ectosome 1 is simultaneously reserved with insert port in center.
Liner 2 forms insulating space between the liner and ectosome from the insert port vertically in the ectosome 1,
There is the space for accommodating fixer, 2 upper end opening of liner in the liner 2.
Lid 3 comprising cover board 4 and vertical part 5, the vertical part 5 are vertically connected to the cover board 4, and the lid of lid 3 is bonded to
When 1 upper end of ectosome, the vertical part 5 is extend into the liner 2, and the cover board 4 seals the upper end opening of the liner 2
It closes, wherein the lower end of the vertical part 5 is provided with the basket 6 for placing Paris polyphylla root tuber sample 7, the side wall setting of the basket 6
There is mesh to make the inside and outside connection of basket 6.
Aspiration pump 8 is connected to the upper portion side wall of the liner 2 by tracheae 9, and the tracheae 9 is in the insulating space
Interior U-shaped setting, the U-shaped bottom of the tracheae form liquid deposition portion, and the liquid deposition portion is provided with the extension of drain pipe 10
To ectosome 1, valve body is provided on the drain pipe 10
Infrared lamp 11, is arranged in the insulating space and towards the liner 2, the side wall of liner be it is transparent, with
Just infrared lamp is irradiated on Paris polyphylla root tuber sample through side wall.
Control module comprising for measuring the baroceptor of liner air pressure inside, for incuding liner internal temperature
Temperature sensor, controller；The controller is connect with baroceptor, temperature sensor, aspiration pump and infrared lamp, institute
Controller aspiration pump according to the pressure control of baroceptor is stated, institute is controlled according to the temperature signal of the temperature sensor
State infrared lamp.
In use, being packed into fixer in liner, after Paris polyphylla root tuber sample is placed into basket, lid is inserted into interior
In gallbladder, lid closes liner upper end opening, then starts aspiration pump and infrared lamp handles Paris polyphylla root tuber sample, finally
Paris polyphylla root tuber sample after being fixed.
Embodiment 1
A kind of paraffin section method of Paris polyphylla root tuber, wherein include:
By Paris polyphylla root tuber sample successively by fixation, dehydration, transparent, waxdip, embedding, slice, open up piece, dewaxing, dyeing and
The paraffin section of the Paris polyphylla root tuber is obtained after mounting；
Wherein, the method for the fixation are as follows: Paris polyphylla root tuber sample is placed in the environment of fixer, negative pressure and infrared radiation
In 24 hours.
Embodiment 2
A kind of paraffin section method of Paris polyphylla root tuber, wherein include:
By Paris polyphylla root tuber sample successively by fixation, dehydration, transparent, waxdip, embedding, slice, open up piece, dewaxing, dyeing and
The paraffin section of the Paris polyphylla root tuber is obtained after mounting；
Wherein, the method for the fixation are as follows: Paris polyphylla root tuber sample is placed in the environment of fixer, negative pressure and infrared radiation
In 24 hours.
The fixer is FAA fixer, 70% ethyl alcohol in FAA fixer: formalin: glacial acetic acid=90:5:5, institute
It states and is mixed with 5 milliliters of glycerine in FAA fixer；
The negative pressure is gradually to be promoted from -0.01 to -0.09Mpa: the length, width and height of the Paris polyphylla root tuber sample are 5 lis
Rice；The infrared radiation is the Infrared irradiation of wavelength 600nm.
Embodiment 3
A kind of paraffin section method of Paris polyphylla root tuber, wherein include:
By Paris polyphylla root tuber sample successively by fixation, dehydration, transparent, waxdip, embedding, slice, open up piece, dewaxing, dyeing and
The paraffin section of the Paris polyphylla root tuber is obtained after mounting；
Wherein, the method for the fixation are as follows: Paris polyphylla root tuber sample is placed in the environment of fixer, negative pressure and infrared radiation
In 24 hours.
The fixer is FAA fixer, 70% ethyl alcohol in FAA fixer: formalin: glacial acetic acid=90:5:5, institute
It states and is mixed with 5 milliliters of glycerine in FAA fixer；
The negative pressure changes in -0.01~-0.09Mpa range: the length, width and height of the Paris polyphylla root tuber sample are 5 lis
Rice；The infrared radiation is the Infrared irradiation of wavelength 800nm.
Wherein, the negative pressure is in following mechanical periodicity: being kept for 5 minutes under -0.01Mpa；15 points are kept under -0.03Mpa
Clock；It is kept for 20 minutes under -0.05Mpa；It is kept for 15 minutes under -0.07Mpa；It is kept for 5 minutes under -0.09Mpa, so as to form one
Period simultaneously recycles until fixation terminates.
Embodiment 4
A kind of paraffin section method of Paris polyphylla root tuber, wherein include:
By Paris polyphylla root tuber sample successively by fixation, dehydration, transparent, waxdip, embedding, slice, open up piece, dewaxing, dyeing and
The paraffin section of the Paris polyphylla root tuber is obtained after mounting；
Wherein, the method for the fixation are as follows: Paris polyphylla root tuber sample is placed in the environment of fixer, negative pressure and infrared radiation
In 24 hours.
The fixer is FAA fixer, 70% ethyl alcohol in FAA fixer: formalin: glacial acetic acid=90:5:5, institute
It states and is mixed with 5 milliliters of glycerine in FAA fixer；
The negative pressure is to change in -0.01~-0.09Mpa range: the length, width and height of the Paris polyphylla root tuber sample are 5 lis
Rice；The infrared radiation is the Infrared irradiation of wavelength 1000nm.
The negative pressure is in following mechanical periodicity: being kept for 5 minutes under -0.01Mpa；It is kept for 15 minutes under -0.03Mpa；-
It is kept for 20 minutes under 0.05Mpa；It is kept for 15 minutes under -0.07Mpa；It is kept for 5 minutes under -0.09Mpa, so as to form a cycle
And it recycles until fixation terminates.
The step of dehydration are as follows: by Paris polyphylla root tuber sample according to 50%, 70%, 85%, 95% ethyl alcohol in cryopreservation tube
Respectively dehydration is primary step by step for concentration, each 1.5h, places into 95% ethanol dehydration 1h, and last 100% ethanol dehydration is twice, each every time
30min。
The transparent step are as follows: at first using+1/2 environment friendly transparent agent of 1/2 dehydrated alcohol to Paris polyphylla root tuber sample clear
Reason is twice；Paris polyphylla root tuber sample clear is handled twice with environment friendly transparent agent again；The time of each transparent processing is 1 hour.
The step of waxdip are as follows: Paris polyphylla root tuber sample is put into ampoule bottle, the dipped Paris polyphylla block of environment friendly transparent agent is added
Root sample, by environment friendly transparent agent: rubble wax is added in the mass ratio of rubble wax=1:0.3, after rubble wax is completely dissolved, then by same
2 rubble waxes are added in the mass ratio of sample, are then put into ampoule bottle in 37 DEG C of baking ovens 6 hours；Then oven temperature is increased to
42 DEG C, it is added three times rubble wax, until saturation；Oven temperature is increased to 60 DEG C again, then is added 3 times by same mass ratio
It is kept for 6 hours after rubble wax；Then Paris polyphylla root tuber sample is transferred in pure liquid paraffin, after being kept for 4 hours, after can carrying out
Continuous embedding；
Wherein, the rubble wax the preparation method comprises the following steps: by new wax and using old wax more than three times according to the quality of 1:1
It than mixing, is subsequently placed in 85 DEG C of baking oven and sufficiently dissolves, be cooled and shaped after the nylon net filter using 300 mesh, then cut broken obtain
To the rubble wax.
The step of embedding are as follows: sufficiently dissolution obtains liquid wax in 85 DEG C of baking oven by the rubble wax, by Paris polyphylla
Root tuber sample is put into the liquid wax, after liquid wax surface condensation, is immediately placed in pre-prepd ice cold water, is promoted
Wax is rapidly solidificated into type.
The thin slice with a thickness of 10 microns is obtained using microtome；It dewaxes again to the thin slice, the dewaxing
Step are as follows: environment friendly transparent agent 10min；1/2 environment friendly transparent agent and 1/2 pure dehydrated alcohol 3min；100%, 95%, 85%, 70%,
50% alcohol and distilled water each 5 minutes.
The step of dyeing are as follows: the thin slice after dewaxing is put in 0.5% sarranine aqueous solution and dyes 15h, distillation washing
Remove extra dye liquor, pass through 30%, 50%, 70%, 80%, 95% dehydration of alcohol respectively, every grade 5 minutes, then it is 0.1% fast green with containing
95% alcohol redye 10 seconds.
Embodiment 5
A kind of paraffin section method of Paris polyphylla root tuber, wherein include:
By Paris polyphylla root tuber sample successively by fixation, dehydration, transparent, waxdip, embedding, slice, open up piece, dewaxing, dyeing and
The paraffin section of the Paris polyphylla root tuber is obtained after mounting；
Wherein, the method for the fixation are as follows: be fixed using the special fixing device, liner is full of fixer
Afterwards, Paris polyphylla root tuber sample is placed in the environment of fixer, negative pressure and infrared radiation 24 hours.
The fixer is FAA fixer, 70% ethyl alcohol in FAA fixer: formalin: glacial acetic acid=90:5:5, institute
It states and is mixed with 5 milliliters of glycerine in FAA fixer；
The negative pressure is to change in -0.01~-0.09Mpa range: the length, width and height of the Paris polyphylla root tuber sample are 5 lis
Rice；The infrared radiation is the Infrared irradiation of wavelength 1000nm.
The negative pressure is in following mechanical periodicity: being kept for 5 minutes under -0.01Mpa；It is kept for 15 minutes under -0.03Mpa；-
It is kept for 20 minutes under 0.05Mpa；It is kept for 15 minutes under -0.07Mpa；It is kept for 5 minutes under -0.09Mpa, so as to form a cycle
And it recycles until fixation terminates.
The step of dehydration are as follows: by Paris polyphylla root tuber sample according to 50%, 70%, 85%, 95% ethyl alcohol in cryopreservation tube
Respectively dehydration is primary step by step for concentration, each 2h, places into 95% ethanol dehydration 1h, and last 100% ethanol dehydration is twice, each every time
45min。
The transparent step are as follows: at first using+1/2 environment friendly transparent agent of 1/2 dehydrated alcohol to Paris polyphylla root tuber sample clear
Reason is twice；Paris polyphylla root tuber sample clear is handled twice with environment friendly transparent agent again；The time of each transparent processing is 2 hours.
The step of waxdip are as follows: Paris polyphylla root tuber sample is put into ampoule bottle, the dipped Paris polyphylla block of environment friendly transparent agent is added
Root sample, by environment friendly transparent agent: rubble wax is added in the mass ratio of rubble wax=1:0.3, after rubble wax is completely dissolved, then by same
3 rubble waxes are added in the mass ratio of sample, are then put into ampoule bottle in 37 DEG C of baking ovens 12 hours；Then oven temperature is increased to
42 DEG C, point 4 addition rubble waxes, until saturation；Oven temperature is increased to 60 DEG C again, then is added 4 times by same mass ratio
It is kept for 12 hours after rubble wax；Then Paris polyphylla root tuber sample is transferred in pure liquid paraffin, after being kept for 4 hours, can be carried out
Subsequent embedding；
Wherein, the rubble wax the preparation method comprises the following steps: by new wax and using old wax more than three times according to the quality of 1:1
It than mixing, is subsequently placed in 85 DEG C of baking oven and sufficiently dissolves, be cooled and shaped after the nylon net filter using 300 mesh, then cut broken obtain
To the rubble wax.
The step of embedding are as follows: sufficiently dissolution obtains liquid wax in 85 DEG C of baking oven by the rubble wax, by Paris polyphylla
Root tuber sample is put into the liquid wax, after liquid wax surface condensation, is immediately placed in pre-prepd ice cold water, is promoted
Wax is rapidly solidificated into type.
The thin slice with a thickness of 14 microns is obtained using microtome；It dewaxes again to the thin slice, the dewaxing
Step are as follows: environment friendly transparent agent 10min；1/2 environment friendly transparent agent and 1/2 pure dehydrated alcohol 3min；100%, 95%, 85%, 70%,
50% alcohol and distilled water each 10 minutes.
The step of dyeing are as follows: the thin slice after dewaxing is put in 15~20h of dyeing, distilled water in 1% sarranine aqueous solution
Wash away extra dye liquor, respectively pass through 30%, 50%, 70%, 80%, 95% dehydration of alcohol, every grade 5 minutes, then with containing 0.1% consolidate
95% green alcohol is redyed 40 seconds.
Comparative example 1
Paris polyphylla root tuber is made using traditional paraffin section preparation methods to be sliced, wherein conventional paraffin section method include it is fixed,
Dehydration, transparent, waxdip, embedding, slice, exhibition piece, roasting piece, dewaxing, rehydration, dyeing, mounting technique.
It is compared being sliced made from slice made from comparative example and embodiment 5, as a result as shown in Figures 3 and 4, Fig. 3 is
Comparative example 1 is the bar=100 μm of electron microscope picture for being 100 microns in scale, and Fig. 4 is at bar=100 μm for embodiment 5
The electron microscope picture that scale is 100 microns.
From figure 3, it can be seen that root skin confluent monolayer cells are sufficiently complete in the slice of Paris polyphylla root block made from traditional paraffin section preparation methods,
Profile is not clear enough；And the root skin confluent monolayer cells of the Paris polyphylla root block slice in Fig. 4 are complete, it is clear-cut, it is seen that the embodiment of the present invention 5
Dicing effect obtained is more preferable.
Although the embodiments of the present invention have been disclosed as above, but its is not only in the description and the implementation listed
With.It can be applied to various suitable the field of the invention completely.It for those skilled in the art, can be easily
Realize other modification.
Claims (10)
1. a kind of paraffin section method of Paris polyphylla root tuber, wherein include:
By Paris polyphylla root tuber sample successively by fixation, dehydration, transparent, waxdip, embedding, slice, exhibition piece, dewaxing, dyeing and mounting
The paraffin section of the Paris polyphylla root tuber is obtained afterwards；
Wherein, the method for the fixation are as follows: Paris polyphylla root tuber sample is placed in 24 in the environment of fixer, negative pressure and infrared radiation
Hour or more.
2. the paraffin section method of Paris polyphylla root tuber as described in claim 1, wherein the fixer is FAA fixer, described
Glycerine is mixed in FAA fixer；
The negative pressure is -0.01~-0.09Mpa: the length, width and height of the Paris polyphylla root tuber sample are 5 centimetres；
The infrared radiation is the Infrared irradiation of 600~1000nm of wavelength.
3. the paraffin section method of Paris polyphylla root tuber as claimed in claim 2, wherein the negative pressure is in following mechanical periodicity:
It is kept for 5 minutes under -0.01Mpa；It is kept for 15 minutes under -0.03Mpa；It is kept for 20 minutes under -0.05Mpa；Under -0.07Mpa
It is kept for 15 minutes；It is kept for 5 minutes under -0.09Mpa, so as to form a cycle and is recycled until fixation terminates.
4. the paraffin section method of Paris polyphylla root tuber as described in claim 1, wherein the step of the dehydration are as follows: by Paris polyphylla block
According to 50%, 70%, 85%, 95% concentration of alcohol, respectively dehydration is primary step by step in cryopreservation tube for root sample, every time 1.5~2h, then
Be put into 95% ethanol dehydration 1h, last 100% ethanol dehydration twice, each each 30~45min.
5. the paraffin section method of Paris polyphylla root tuber as claimed in claim 4, wherein the transparent step are as follows: first use 1/2
+ 1/2 environment friendly transparent agent of dehydrated alcohol handles twice Paris polyphylla root tuber sample clear；
Paris polyphylla root tuber sample clear is handled twice with environment friendly transparent agent again；
The time of each transparent processing is 1~2 hour.
6. the paraffin section method of Paris polyphylla root tuber as claimed in claim 5, wherein the step of the waxdip are as follows:
Paris polyphylla root tuber sample is put into ampoule bottle, the dipped Paris polyphylla root tuber sample of environment friendly transparent agent is added, by environment friendly transparent agent: broken
Rubble wax is added in the mass ratio of paraffin=1:0.3, broken after rubble wax is completely dissolved, then by the addition of same mass ratio 2~3 times
Then ampoule bottle is put into 37 DEG C of baking ovens 6~12 hours by paraffin；
Then oven temperature is increased to 42 DEG C, divides 3-4 addition rubble wax, until saturation；
Oven temperature is increased to 60 DEG C again, then is kept for 6~12 hours after 3-4 rubble wax is added by same mass ratio；
Then Paris polyphylla root tuber sample is transferred in pure liquid paraffin, after being kept for 4 hours, subsequent embedding can be carried out；
Wherein, the rubble wax the preparation method comprises the following steps: by new wax and old wax more than three times was used to mix according to the mass ratio of 1:1
It closes, is subsequently placed in 85 DEG C of baking oven and sufficiently dissolves, be cooled and shaped after the nylon net filter using 300 mesh, then cut and broken obtain institute
State rubble wax.
7. the paraffin section method of Paris polyphylla root tuber as claimed in claim 6, wherein the step of the embedding are as follows:
By the rubble wax, sufficiently dissolution obtains liquid wax in 85 DEG C of baking oven, and Paris polyphylla root tuber sample is put into the liquid
It in wax, after liquid wax surface condensation, is immediately placed in pre-prepd ice cold water, wax is promoted to be rapidly solidificated into type.
8. the paraffin section method of Paris polyphylla root tuber as claimed in claim 7, wherein obtained using microtome with a thickness of 10
~14 microns of thin slice；
It dewaxes again to the thin slice, the step of the dewaxing are as follows: environment friendly transparent agent 10min；1/2 environment friendly transparent agent and 1/2
Pure dehydrated alcohol 3min；100%, 95%, 85%, 70%, 50% alcohol and distilled water each 5~10 minutes.
9. the paraffin section method of Paris polyphylla root tuber as claimed in claim 8, wherein the step of the dyeing are as follows:
Thin slice after dewaxing is put in 15~20h of dyeing, distilled water in 0.5%-1% sarranine aqueous solution and washes away extra dye liquor, point
Not Jing Guo 30%, 50%, 70%, 80%, 95% dehydration of alcohol, every grade 5 minutes, then with multiple containing 0.1% 95% fast green alcohol
Dye 10~40 seconds.
10. the paraffin section method of Paris polyphylla root tuber as claimed in claim 3, wherein using described in special fixing device progress
Fixing step, the special fixing device include:
Ectosome, internal to have accommodation space, the upper end closed of the ectosome is simultaneously reserved with insert port in center；
Liner, from the insert port vertically to it is described it is outer form insulating space between the liner and ectosome in vivo, it is described in
There is the space for accommodating fixer, the liner upper end opening in gallbladder；
Lid comprising cover board and vertical part, the vertical part are vertically connected to the cover board, and the cover cap is bonded on the ectosome
When end, the vertical part is extend into the liner, and the cover board closes the upper end opening of the liner, wherein the vertical part
Lower end is provided with the basket for placing Paris polyphylla root tuber sample, and the side wall of the basket is provided with mesh and to connect inside and outside basket
It is logical；
Aspiration pump is connected to the upper portion side wall of the liner by tracheae, and the tracheae is U-shaped in the insulating space to be set
It sets, the U-shaped bottom of the tracheae forms liquid deposition portion, and the liquid deposition portion is provided with drain pipe and stretches to ectosome,
Valve body is provided on the drain pipe；
Infrared lamp is arranged in the insulating space and towards the liner；
Control module comprising the baroceptor for measuring liner air pressure inside, the temperature for incuding liner internal temperature
Spend sensor, controller；The controller is connect with baroceptor, temperature sensor, aspiration pump and infrared lamp, the control
Device processed aspiration pump according to the pressure control of baroceptor controls described red according to the temperature signal of the temperature sensor
Outer lamp.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110006721A (en) * | 2019-03-26 | 2019-07-12 | 广西壮族自治区药用植物园 | The slice preparation method of kadsura root |
| CN110779758A (en) * | 2019-11-01 | 2020-02-11 | 上海市农业科学院 | Preparation method of shiitake mushroom paraffin section |
| CN112945657A (en) * | 2021-01-22 | 2021-06-11 | 中国科学院东北地理与农业生态研究所 | Pretreatment device and method for preparing plant material paraffin section |
Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202075161U (en) * | 2011-03-07 | 2011-12-14 | 长沙康柏恩医疗科技有限公司 | Quick microwave biological tissue processing apparatus |
| CN102768145A (en) * | 2012-06-29 | 2012-11-07 | 长沙康柏恩医疗科技有限公司 | Method for fast flaking paraffin by microwave radiation water bath diathermy and device |
| CN105606437A (en) * | 2016-03-01 | 2016-05-25 | 青岛锴锦医疗器械有限公司 | Rapid pathological tissue dehydrating machine |
| CN105806682A (en) * | 2016-03-08 | 2016-07-27 | 中国环境科学研究院 | Method for preparing leguminous plant leaf paraffin section |
| CN106501053A (en) * | 2016-12-30 | 2017-03-15 | 广西壮族自治区药用植物园 | The paraffin section method of meadowrueleaf corydalis root blade |
| CN106501043A (en) * | 2016-10-26 | 2017-03-15 | 中国热带农业科学院椰子研究所 | A kind of paraffin section method of effective observation oil palm gynoecium anatomical structure |
| CN206705877U (en) * | 2017-02-23 | 2017-12-05 | 天津德清科技有限公司 | The retracting device of aromatic compound in a kind of organic wastewater |
| CN107702959A (en) * | 2017-10-27 | 2018-02-16 | 陕西省西安植物园 | A kind of paraffin section method for vegetable material serial dehydration |
| CN207957821U (en) * | 2018-01-09 | 2018-10-12 | 天津纳万源科技发展有限公司 | A kind of constant-pressure water supplementing vacuum degassing group |
| CN109060468A (en) * | 2018-06-11 | 2018-12-21 | 塔里木大学 | A kind of preparation method of the paraffin section of pear flower tissue |
| CN208511906U (en) * | 2018-05-31 | 2019-02-19 | 山西豪仑科化工有限公司 | One kind is for gaseous condensate aggregate device under different condition of negative pressure |
| CN109632425A (en) * | 2019-01-11 | 2019-04-16 | 北京林业大学 | A kind of paraffin section production method of Chinese herbaceous peony underground rhizome |
-
2019
- 2019-04-25 CN CN201910338345.0A patent/CN109991063A/en active Pending
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202075161U (en) * | 2011-03-07 | 2011-12-14 | 长沙康柏恩医疗科技有限公司 | Quick microwave biological tissue processing apparatus |
| CN102768145A (en) * | 2012-06-29 | 2012-11-07 | 长沙康柏恩医疗科技有限公司 | Method for fast flaking paraffin by microwave radiation water bath diathermy and device |
| CN105606437A (en) * | 2016-03-01 | 2016-05-25 | 青岛锴锦医疗器械有限公司 | Rapid pathological tissue dehydrating machine |
| CN105806682A (en) * | 2016-03-08 | 2016-07-27 | 中国环境科学研究院 | Method for preparing leguminous plant leaf paraffin section |
| CN106501043A (en) * | 2016-10-26 | 2017-03-15 | 中国热带农业科学院椰子研究所 | A kind of paraffin section method of effective observation oil palm gynoecium anatomical structure |
| CN106501053A (en) * | 2016-12-30 | 2017-03-15 | 广西壮族自治区药用植物园 | The paraffin section method of meadowrueleaf corydalis root blade |
| CN206705877U (en) * | 2017-02-23 | 2017-12-05 | 天津德清科技有限公司 | The retracting device of aromatic compound in a kind of organic wastewater |
| CN107702959A (en) * | 2017-10-27 | 2018-02-16 | 陕西省西安植物园 | A kind of paraffin section method for vegetable material serial dehydration |
| CN207957821U (en) * | 2018-01-09 | 2018-10-12 | 天津纳万源科技发展有限公司 | A kind of constant-pressure water supplementing vacuum degassing group |
| CN208511906U (en) * | 2018-05-31 | 2019-02-19 | 山西豪仑科化工有限公司 | One kind is for gaseous condensate aggregate device under different condition of negative pressure |
| CN109060468A (en) * | 2018-06-11 | 2018-12-21 | 塔里木大学 | A kind of preparation method of the paraffin section of pear flower tissue |
| CN109632425A (en) * | 2019-01-11 | 2019-04-16 | 北京林业大学 | A kind of paraffin section production method of Chinese herbaceous peony underground rhizome |
Non-Patent Citations (1)
| Title |
|---|
| 简兴 等: "三种重楼属(Paris)植物营养器官的比较解剖学研究", 《重庆文理学院学报》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110006721A (en) * | 2019-03-26 | 2019-07-12 | 广西壮族自治区药用植物园 | The slice preparation method of kadsura root |
| CN110006721B (en) * | 2019-03-26 | 2021-10-15 | 广西壮族自治区药用植物园 | Preparation method of slices of tiger's root |
| CN110779758A (en) * | 2019-11-01 | 2020-02-11 | 上海市农业科学院 | Preparation method of shiitake mushroom paraffin section |
| CN112945657A (en) * | 2021-01-22 | 2021-06-11 | 中国科学院东北地理与农业生态研究所 | Pretreatment device and method for preparing plant material paraffin section |
| CN112945657B (en) * | 2021-01-22 | 2023-08-25 | 中国科学院东北地理与农业生态研究所 | Pretreatment device and method for preparing plant material paraffin section |
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