CN109988715B - Mutant strain for high-yield xylanase and application thereof - Google Patents

Mutant strain for high-yield xylanase and application thereof Download PDF

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CN109988715B
CN109988715B CN201711477714.1A CN201711477714A CN109988715B CN 109988715 B CN109988715 B CN 109988715B CN 201711477714 A CN201711477714 A CN 201711477714A CN 109988715 B CN109988715 B CN 109988715B
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xylanase
trichoderma reesei
fermentation
mutant strain
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CN109988715A (en
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刘士成
李�瑞
王华明
曹兴南
王贵斌
宋雅丽
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Weifang Kdn Biotech Co ltd
Qingdao Vland Biotech Group Co Ltd
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Qingdao Vland Biotech Group Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/145—Fungal isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/885—Trichoderma

Abstract

The invention belongs to the technical field of beneficial microorganism modification and screening, and particularly relates to a trichoderma reesei mutant strain for high xylanase yield and application thereof. The preservation number of the mutant strain is CCTCC NO: M2017797. After the mutant strain is subjected to shake flask fermentation for 120 hours, the xylanase enzyme activity in the fermentation supernatant reaches 1500U/mL, is improved by 80% compared with that of the original strain, and unexpected technical effects are achieved. The Trichoderma reesei mutant strain provided by the invention can be widely applied to fermentation production of xylanase, is beneficial to reducing the production cost of the xylanase and promotes the popularization and application of the xylanase.

Description

Mutant strain for high-yield xylanase and application thereof
Technical Field
The invention belongs to the technical field of beneficial microorganism modification and screening, and particularly relates to a trichoderma reesei mutant strain for high xylanase yield and application thereof.
Background
Xylan is the major component of hemicellulose, the major polysaccharide structure of plant cells, 1/3 accounting for all renewable organic carbons. Xylans are usually present in the secondary walls of plants, in large amounts in hardwood in angiosperms (15-30% cell wall components), in softwood in gymnosperms (7-10% cell wall components) and in annual plants (< 30% cell wall components). In the cell wall, xylan is present at the interface of cellulose and lignin, which is important for the fiber cohesion and integrity of the plant cell wall.
The xylanase is a complex enzyme system capable of degrading xylan into xylo-oligosaccharide or xylose, and mainly comprises endo-beta-L, 4-D-xylanase, beta-D-xylosidase, alpha-L-arabinofuranosidase, alpha-D-glucuronidase, acetyl xylan esterase and phenolic acid esterase. Xylanases from different sources vary in the degree of polymerization of the backbone, the number, type, length of branches, and their binding sites. During the degradation process of xylan, the exonuclease and the endonuclease are mutually promoted, so that the degradation process of xylan is accelerated.
At present, there are 3 main routes for xylanase production: 1) screening microorganisms capable of secreting novel hydrolases; 2) the current industrial strain is modified by enzyme engineering means; 3) some influencing factors, such as substrate types, culture conditions, recycling of enzyme preparations and redesign of production flow, are optimized in the production process.
The microorganisms capable of secreting xylanase, which can be applied to production, are mainly fungi and bacteria, wherein the fungi mainly comprise aspergillus, penicillium and the like, and the bacteria mainly comprise streptomyces, bacillus and the like. For example, Penicillium Pol6 is characterized by acidic optimal reaction conditions for secreted extracellular xylanases and may be used in more extreme environments, such as in the feed and food fields. The xylanase produced by Aspergillus oryzae HML366 has good thermal stability and pH tolerance, and can be applied to the fields of papermaking and biological energy. Xylanase secreted by streptomycete CS624 and bacillus pumilus SS1 can be used for efficiently performing enzymolysis on wheat bran to generate xylo-oligosaccharide. The Streptomyces isothioicus LMZM is subjected to liquid fermentation by taking corncobs as substrates, and the produced xylanase can improve the brightness of paper when being used for bleaching paper pulp. The xylanase produced by the fibrobacter CKMX1 has stable activity at the pH value of 5.0-9.0 and the temperature of 50-60 ℃, and can be widely applied to the field of pulping and papermaking.
With the continuous development of genetic engineering technology, xylanase genes can be expressed in expression systems of escherichia coli, bacillus, yeast and filamentous fungi. For example, XynA gene is cloned from the schizophyllum thermophilum and expressed in pichia pastoris X-33, the recombinase protein can maintain 60 percent of enzyme activity in the environment with pH6.0-9.0 and 60-80 ℃, and the enzyme has larger application potential due to higher tolerance to pH and temperature. The xylanase gene Xyn11A cloned from bacillus encodes 366 amino acids, and the optimal reaction temperature of the recombinant protease is 55 ℃. The XynB gene of the Aspergillus niger IA-001 is optimized and cloned into Pichia pastoris GS115, the activity of the optimized recombinant protein is improved by 2.8 times compared with that of a wild enzyme, and the recombinant protein can express higher xylanase activity.
The invention obtains the production strain with improved xylanase activity by screening through a mutagenesis method, so as to adapt to industrial large-scale production and promote the further development of xylanase.
Disclosure of Invention
The invention aims to provide a Trichoderma reesei (Trichoderma reesei) mutant strain and application thereof in xylanase production. The mutant strain obtained by screening by the applicant through an ultraviolet mutagenesis method can greatly improve the expression quantity of the xylanase, can be widely applied to the production of the xylanase, reduces the cost of the xylanase and is beneficial to the wide application of the xylanase.
The invention provides a mutant strain Trichoderma reesei Mu17(Trichoderma reesei Mu17), which is preserved in China center for type culture Collection of Wuhan university in Wuhan, China at 12 and 15 months in 2017, and the preservation number is CCTCC NO: M2017797.
The invention provides an application of the trichoderma reesei in xylanase production.
The invention also provides a method for producing xylanase, which takes the trichoderma reesei as a fermentation strain.
The invention also provides xylanase obtained by fermenting the trichoderma reesei.
The invention takes the trichoderma reesei Mu as an original strain, and obtains a mutant strain trichoderma reesei Mu17 by screening through an ultraviolet mutagenesis method. After the mutant strain is subjected to shake flask fermentation for 120 hours, the xylanase enzyme activity in the fermentation supernatant reaches 1500U/mL, is improved by 80% compared with that of the original strain, and unexpected technical effects are achieved. Mutant strain Mu17 has obvious change on the bacterial colony phenotype with starting the fungus relatively, and mutant strain Mu 17's bacterial colony obviously diminishes, and the bacterial colony diameter only is for going out the 1/2 of fungus, and the bacterial colony is more closely knit, is favorable to reducing fermentation broth viscosity, improves the dissolved oxygen content in the fermentation process, and then is favorable to improving the biomass, increases the secretory expression volume of foreign protein. The Trichoderma reesei mutant strain provided by the invention can be widely applied to fermentation production of xylanase, is beneficial to reducing the production cost of the xylanase and promotes the popularization and application of the xylanase.
Drawings
FIG. 1 is a diagram showing a comparison of colony morphology between an original bacterium Mu and a mutant bacterium Mu 17.
Detailed Description
The present invention uses conventional techniques and methods used IN the fields of genetic engineering and MOLECULAR BIOLOGY, such as the methods described IN MOLECULAR CLONING, A LABORATORY MANUAL,3nd Ed. (Sambrook,2001) and CURRENT PROTOCOLS IN MOLECULAR BIOLOGY (Ausubel, 2003). These general references provide definitions and methods known to those skilled in the art. However, it is not intended that the invention be limited to any particular methodology, protocols, and reagents described, as these may vary.
The present invention will be described in detail with reference to specific embodiments.
Example 1 Shake flask fermentation and enzyme Activity detection of Trichoderma reesei Mu
The Trichoderma reesei mu (Trichoderma reesei mu) strain is a Trichoderma reesei engineering strain which is constructed by transforming xylanase genes derived from Trichoderma reesei into Trichoderma reesei host cells and is used for expressing the xylanase. The applicant has used this strain for fermentation to produce xylanase, but during use this strain was found to have a problem of reduced enzyme production activity. Therefore, the applicant conducted mutagenesis using the Trichoderma reesei Mu strain as an original strain and screened mutant strains suitable for large-scale culture and having high enzyme production levels.
The applicant firstly inoculated Trichoderma reesei Mu to a fresh PDA plate (potato 200g/L, boiled for 20-30min, filtered to remove residues; glucose 2%, agar powder 1.5%), and cultured at 30 ℃ for 7 d.
Eluting with 5ml sterile water to obtain spore liquid, inoculating 50ml MM fermentation medium (1.5% glucose, 4% liquid sugar, 2.5% corn steep liquor, 0.44% (NH)4)2SO4，0.09％MgSO4，2％KH2PO4，0.04％CaCl20.018% Tween-80, 0.018% microelement and 0.018% polypropylene glycol-2000), culturing at 28 deg.C for 120 hr, and centrifuging to obtain fermentation supernatant. And (4) carrying out enzyme activity determination on the fermentation supernatant. The result shows that the xylanase activity in the fermentation supernatant is 833U/mL.
Enzyme activity measuring method
(1) Definition of xylanase Activity Unit
The enzyme amount required for releasing 1 mu mol of reducing sugar from 5mg/ml xylan solution per minute at 37 ℃ and pH5.5 is an enzyme activity unit U.
(2) Enzyme activity measuring method
Taking 2ml of xylan substrate with the concentration of 1% (prepared by a pH5.5 acetic acid-sodium acetate buffer solution), adding the xylan substrate into a colorimetric tube, balancing for 10min at 37 ℃, adding 2ml of acidic xylanase enzyme solution which is properly diluted by the pH5.5 acetic acid-sodium acetate buffer solution and well balanced at 37 ℃, uniformly mixing the acidic xylanase enzyme solution and the acidic xylanase enzyme solution, and carrying out accurate heat preservation reaction at 37 ℃ for 30min is the same as the formula (I). After the reaction was completed, 5ml of DNS reagent was added and mixed well to terminate the reaction. Boiling in boiling water bath for 5min, cooling to room temperature with tap water, adding distilled water to constant volume to 25ml, mixing, measuring absorbance A at 540nm with standard blank as blank controlE。
The enzyme activity calculation formula is as follows:
XD＝[(AE－AB)×K+C0]×N×1000/(M×t)
in the formula: xDFor the activity of xylanase in the diluted enzyme solution, U/ml; a. theEThe absorbance of the enzyme reaction solution; a. theBThe absorbance of the enzyme blank liquid; k is the slope of the standard curve; c0Is the intercept of the standard curve; m is the molar mass of xylose, 150.2 g/mol; t is enzymolysis reaction time, min; n is the dilution multiple of enzyme solution; 1000 is conversion factor, 1mmol ═ 1000 μmol.
Example 2 UV mutagenesis screening
The applicant further screens mutant strains with improved xylanase yield by using trichoderma reesei Mu as an original strain through an ultraviolet mutagenesis method.
Determination of the lethality rate: inoculating the Trichoderma reesei Mu to a PDA plate, and culturing at 30 ℃ for 7 d. When a large amount of spores are generated on the surface of the colony, 5ml of sterile water is absorbed for elution to obtain a spore liquid, the spore liquid is resuspended by the sterile water after centrifugation, and a blood counting chamber is used for counting. A90 mm petri dish was taken and 5ml of diluted spore suspension (concentration about 1X 10) was added7one/mL) was added to the vessel and stirred on a magnetic stirrer to make the spore liquid homogeneous. Irradiating with ultraviolet lamp with power of 9w at a vertical distance of 20cm in a sterile ultra-clean bench for 30s, 60s, 90s, 120s, 150s, and 180s, respectively, diluting the irradiated spore solution for 10, 100, and 1000 times, coating 100ul PDA plate with the diluted spore solution, culturing at 30 deg.C for 2-3d, counting, and calculating lethality with unirradiated spore solution as control. Wherein the lethality is 98% when the irradiation time is 150s, and the irradiation time is selected for subsequent mutagenesis experiments.
Mutagenesis screening: a90 mm petri dish was taken and 5ml of diluted spore suspension (concentration 1X 10) was added7one/mL), add the rotor and on a magnetic stirrerStirring to make spore liquid in uniform state. Irradiating with ultraviolet lamp with power of 9w at a vertical distance of 25cm in a sterile ultra-clean workbench for 150s, closing the ultraviolet lamp tube, covering the upper cover of the culture dish, and standing in dark for 20 min. The UV-irradiated spore suspension was then diluted 100-fold, 200ul of the suspension was plated on a PDA plate, 20 PDA plates were plated in each batch, and the batch was incubated at 30 ℃ for 2 days. Firstly, morphologically observing colonies growing on a PDA plate, selecting 34 mutant strains with obviously reduced colonies, respectively inoculating the mutant strains on the PDA plate, and culturing at 30 ℃ for 7 d. Eluting each mutant bacterium colony by using 5ml of sterile water to obtain spore liquid; the cells were inoculated into 50ml of MM fermentation medium (1.5% glucose, 4% liquid sugar, 2.5% corn steep liquor, 0.44% (NH)4)2SO4，0.09％MgSO4，2％KH2PO4，0.04％CaCl20.018% Tween-80, 0.018% microelement and 0.018% polypropylene glycol-2000), and culturing at 28 deg.C for 120 hr; centrifuging to obtain fermentation supernatant.
Through xylanase enzyme activity detection on the obtained fermentation supernatant, the applicant finally screens out a mutant strain with the highest xylanase yield, namely Trichoderma reesei Mu17(Trichoderma reesei Mu17), the xylanase enzyme activity in the fermentation supernatant of the strain reaches 1500U/mL, is improved by 80% compared with that of the original strain, and unexpected technical effects are achieved.
Compared with the growth bacteria Mu, the mutant bacteria Mu17 have obvious change on the colonial phenotype (as shown in figure 1), the colonial of the mutant bacteria Mu17 is obviously reduced, the diameter of the colonial is only 1/2 of the growth bacteria, and the colonial is more compact, so that the viscosity of the fermentation liquid is reduced, the dissolved oxygen content in the fermentation process is improved, the biomass is further improved, and the secretion expression amount of the exogenous protein is increased.
The mutant strain Trichoderma reesei Mu17(Trichoderma reesei Mu17) is preserved in China center for type culture Collection, CCTCC NO: M2017797 by the applicant at 12, 15 and 12 months in 2017.

Claims (4)

1. Trichoderma reesei (II) (A. reesei)Trichoderma reesei) Characterized in thatThe preservation number of the Trichoderma reesei (Trichoderma reesei) is CCTCC NO: M2017797.
2. Use of the trichoderma reesei of claim 1 for the production of xylanase.
3. A method for producing xylanase by fermentation of Trichoderma reesei according to claim 1 as a fermentation strain.
4. The method of claim 3, wherein the fermentation medium used in the fermentation comprises the following composition: 1.5% glucose, 4% liquid sugar, 2.5% corn steep liquor, 0.44% (NH)4)2SO4，0.09％MgSO4，2％KH2PO4，0.04％CaCl20.018% of tween-80, 0.018% of trace elements and 0.018% of polypropylene glycol-2000.
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EP3995567A4 (en) * 2019-07-04 2023-08-09 Shandong Bailong Chuangyuan Bio-tech Co., Ltd Trichoderma reesei strain, culture method therefor, and application thereof
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