CN109985681A - A kind of microlayer model generation device - Google Patents
A kind of microlayer model generation device Download PDFInfo
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- CN109985681A CN109985681A CN201910378203.7A CN201910378203A CN109985681A CN 109985681 A CN109985681 A CN 109985681A CN 201910378203 A CN201910378203 A CN 201910378203A CN 109985681 A CN109985681 A CN 109985681A
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
Abstract
The present invention proposes a kind of microlayer model generation device, including chip and droplet detachment mechanism, it is provided with liquid storage chamber and fluid channel on the chip, is equipped with the first phase liquid in the liquid storage chamber, the fluid channel arrival end is connected to the liquid storage chamber, and the fluid channel outlet end extends to the outer rim of the chip；It is opposite that the drop dropping mechanism and the fluid channel are located at one end of the chip outer rim, is provided with droplet formation gap between the drop releasing mechanism and the fluid channel outlet end；The contact angle of the fluid channel outlet end and the first phase liquid is greater than the contact angle of the drop dropping mechanism and the first phase liquid.Microlayer model generation device of the invention can quickly, low cost formation microlayer model, and formed microlayer model diameter it is uniform.
Description
Technical field
The present invention relates to biology and analytical chemistry and technical field of medical detection, are related to a kind of microlayer model generation dress
It sets.
Background technique
Microlayer model has many advantages, such as that small in size, flux is high, size is uniform, internal stability, controllable, is widely used in giving birth to
The fields such as object, chemical detection analysis, medical diagnosis, materials synthesis.Micro-fluidic chip has that size is small, reagent consumption is few, high pass
The features such as measuring, is easy of integration, the research and analysis suitable for microbody system work, and make the microlayer model technical application based on micro-fluidic chip
There are big advantage and prospect to unicellular research and analysis.
And the drop micro-fluidic chip studied at present, the formation of microlayer model depends in patent CN201710429242.6
Plane micro-fluidic chip, plane micro-fluidic chip generates drop and needs to connect more than two pumps, complicated for operation, and equipment is large-scale,
Said pump and chip contact, there is a possibility that cross contamination；Sample dispersion depends on silicon wafer in patent CN201510338390.8
The processing of upper micropore, this mode higher cost is higher to micropore coherence request, and consumptive material further comprises other than chip
Coated tool；The uniform drop of diameter is unable to get in patent CN201710855088.9.
In view of the difficulty and defect of the practice of above-mentioned drop micro-fluidic chip, it is urgent to provide a kind of drop generating devices to solve
State problem.
Summary of the invention
It is an object of the invention to propose a kind of microlayer model generation device, can quickly, low cost formation microlayer model, and
The microlayer model diameter of formation is uniform.
To achieve this purpose, the present invention adopts the following technical scheme:
A kind of microlayer model generation device, including chip and droplet detachment mechanism are provided with liquid storage chamber and micro- on the chip
Runner, the liquid storage chamber is interior to be equipped with the first phase liquid, and the fluid channel arrival end is connected to the liquid storage chamber, the fluid channel outlet
End extends to the outer rim of the chip；It is opposite that the drop dropping mechanism and the fluid channel are located at one end of the chip outer rim,
Droplet formation gap is provided between the drop releasing mechanism and the fluid channel outlet end；The fluid channel outlet end and institute
The contact angle for stating the first phase liquid is greater than the contact angle of the drop dropping mechanism and the first phase liquid.
Specifically, the first phase liquid flows into fluid channel from liquid storage chamber, then slowly flowed out from one end of chip outer rim, due to
Capillarity, the first phase liquid formed small before this in the fluid channel exit, that is, droplet formation gap of one end of chip outer rim
Drop, then droplet diameter is gradually increased, until touching drop dropping mechanism, i.e. droplet is filled in droplet formation gap
Fluid channel and droplet detachment mechanism are contacted simultaneously, since drop dropping mechanism is located at the outlet end of chip outer rim more with respect to fluid channel
Hydrophilic, then drop can be bonded in droplet detachment mechanism, be fallen off with chip, and microlayer model is formed.
Preferably, the chip and the drop dropping mechanism keep relative motion during droplet formation.
Preferably, the contact angle of the fluid channel outlet end and the first phase liquid than the drop dropping mechanism with it is described
Big 10 ° of contact angle or more of first phase liquid.
It is highly preferred that the contact angle of the fluid channel outlet end and the first phase liquid is than the drop dropping mechanism and institute
The contact angle for stating the first phase liquid is 20-60 ° big.
Preferably, the diameter of the fluid channel is 10-50 μm, and the width in the droplet formation gap is 10-30 μm.That is institute
The distance for stating droplet detachment mechanism to the chip outer rim is 10-30 μm.
Preferably, the chip is disc, and the liquid storage chamber is located at the chip center region, the droplet detachment machine
Structure is the circle concentric with the chip on the inside of the chip, the corresponding droplet detachment mechanism.
Preferably, the chip is connect with centrifugation apparatus, and the centrifugation apparatus provides centrifugal force, the core for the chip
Piece revolving speed is 200-1000rpm.
Preferably, the gas vent through the chip thickness direction is provided on the chip.
Preferably, the microlayer model generation device further includes droplet collection device, and the droplet collection device is located at described
Below droplet detachment mechanism, two phase liquid is equipped in the droplet collection device.
It preferably, is 5-20mm at a distance from chip at the liquid level of the two phase liquid.
Preferably, the microlayer model generation device further includes outer clamp, and the outer clamp is for fixing the drop
Dropping mechanism.
The beneficial effects of the present invention are:
On the one hand microlayer model generation device of the invention passes through the contact angle of setting fluid channel outlet end and the first phase liquid
Contact angle greater than drop dropping mechanism and the first phase liquid glue drop can using the difference of two contact surface hydrophilicity and hydrophobicities
It ties in droplet detachment mechanism, falls off with chip, form microlayer model, have the characteristics that structure is simple and convenient to operate；Another party
Face is immobilized by the way that droplet detachment mechanism width in i.e. droplet formation gap at a distance from chip outer rim is arranged, can be formed
The uniform microlayer model of diameter.
Detailed description of the invention
Fig. 1 is microlayer model generation device schematic diagram of the invention；
Fig. 2 is microlayer model forming process schematic diagram of the invention；
Fig. 3 is another microlayer model generation device schematic diagram of the invention；
Fig. 4 is chip schematic diagram of the invention；
Fig. 5 is another microlayer model generation device schematic diagram of the invention.
In figure, chip 100, liquid storage chamber 110, fluid channel 120, the first phase liquid 130, gas vent 140, droplet detachment mechanism
200, droplet formation gap 210, droplet collection device 300, two phase liquid 310, outer clamp 400.
Specific embodiment
The embodiment of the present invention is described below in detail, examples of the embodiments are shown in the accompanying drawings, wherein from beginning to end
Same or similar label indicates same or similar element or element with the same or similar functions.Below with reference to attached
The embodiment of figure description is exemplary, it is intended to is used to explain the present invention, and is not considered as limiting the invention.
Embodiment 1
Below with reference to the accompanying drawings it is described in detail microlayer model generation device according to an embodiment of the present invention.
As shown in Figure 1, being microlayer model generation device of the invention, including chip 100 and droplet detachment mechanism 200.
In this present embodiment, it is provided with liquid storage chamber 110 and fluid channel 120 on chip 100, is equipped with first in liquid storage chamber 110
Phase liquid 130,120 arrival end of fluid channel are connected to liquid storage chamber 110, and 120 outlet end of fluid channel extends to the outer rim of chip 100.
Specifically, the first phase liquid 130 is added in liquid storage chamber 110, the first phase liquid 130 can enter fluid channel
In 120, since 120 diameter of fluid channel is smaller, under capillary action, the first phase liquid runs down fluid channel 120 is reached outside chip 100
Edge, fluid channel 120 is close to drop 200 side of dropping mechanism and atmosphere, and due to gas pressure, liquid will not Automatic-falling.When
When the first phase liquid in chip 100 is driven power, the first phase liquid 130 can be located at 100 outer rim of chip from fluid channel 120
Outlet end outflow.
100 material of chip includes glass, silicon wafer, quartz or common polymer material.Polymer material includes
Dimethyl silicone polymer (PDMS), polyurethane, epoxy resin, polymethyl methacrylate (PMMA), polycarbonate (PC), cyclenes
Hydrocarbon copolymer (COC), polystyrene (PS), polyethylene (PE), fluoroplastics.100 material of chip can be used in above-mentioned material
It is one or several kinds of.
100 processing method of chip according to material and structure, can choose photoetching, numerical control, casting, injection molding, laser engraving, etc.
One of distinct methods such as ion etching, wet etching are several.
Fluid channel 120 can be direct by above-mentioned conventional micro Process means (photoetching, injection molding, numerical control, laser processing etc.)
It is prepared on chip 100.
Fluid channel 120 can also be prepared by the way that capillary is embedded in advance in chip 100.
In this present embodiment, the diameter of fluid channel 120 is 10-50 μm, such as 10 μm, 20 μm, 30 μm, 40 μm or 50 μm.
In this present embodiment, drop dropping mechanism is opposite with one end that fluid channel 120 is located at 100 outer rim of chip, and drop is detached from
Mechanism 200 and fluid channel are provided with droplet formation gap 210 between 120 outlet end；Fluid channel outlet end and the first phase liquid
Contact angle is greater than the contact angle of drop dropping mechanism and the first phase liquid.
Specifically, as shown in Fig. 2, being microlayer model forming process schematic diagram of the invention.When microlayer model generation device is run,
The effect that first phase liquid 130 is driven power is gradually flowed out from fluid channel 120, and one is formed at the outer rim of chip 100
Small drop, and being incrementally increased under the action of driving force, when liquid-drop diameter reach drop dropping mechanism and 100 outer rim of chip away from
When from reaching the width in droplet formation gap 210, drop touches drop dropping mechanism 200 simultaneously and fluid channel 120 is located at core
The outlet end of 100 outer rim of piece, due to 200 relative hydropathic of droplet detachment mechanism, drop is bonded in droplet detachment mechanism 200, and
Chip 100 falls off, and forms microlayer model.In the present invention, driving force is centrifugal force, the air pump that centrifugation apparatus in the prior art provides
Driving force that the mechanical force of generation or other conventional means provide or other the first phase liquid can be promoted from fluid channel entrance
To the power of outlet end motion.
During droplet formation, there are relative motions for chip 100 and drop dropping mechanism 200, can significantly improve micro- liquid
The rate formed is dripped, and is conducive to microlayer model and is rapidly collected in collection device 300.
In this present embodiment, fluid channel outlet end is with the contact angle of the first phase liquid than drop dropping mechanism and the first phase liquid
Big 10 ° of contact angle or more.For example, fluid channel outlet end and the contact angle ratio drop dropping mechanism of the first phase liquid and the first phase liquid
The contact angle of body is 10 °, 20 °, 30 °, 40 °, 50 °, 60 °, 70 °, 80 °, 90 ° or 100 ° big.
Preferably, 120 outlet end of fluid channel is with the contact angle of the first phase liquid 130 than drop dropping mechanism 200 and the first phase
The contact angle of liquid 130 is 10-80 ° big；It is furthermore preferred that 120 outlet end of fluid channel and the contact angle of the first phase liquid 130 compare drop
Dropping mechanism 200 and the contact angle of the first phase liquid 130 are 20-60 ° big.
In some specific embodiments, 120 outlet end of fluid channel has hydrophobic surface, hydrophobic surface and the first phase liquid
130 contact angle is 80-110 °, and droplet detachment mechanism 200 has water-wetted surface, and water-wetted surface connects with the first phase liquid 130
Feeler is 50-75 °.Thus, it is possible to further ensure that 120 outlet end of fluid channel and the hydrophilic and hydrophobic of droplet detachment mechanism 200 are poor
It is different, it is beneficial to the generation of drop, while there is 120 outlet end of fluid channel hydrophobic surface can also further ensure that microlayer model generates
Device first phase liquid 130 in unused state stays in fluid channel 120, without flowing out from 120 outlet end of fluid channel.
In this present embodiment, the width in droplet formation gap is 10-30 μm, i.e., outside droplet detachment mechanism 200 to chip 100
The distance of edge is 10-30 μm.Specifically, the distance of droplet detachment mechanism 200 to 100 outer rim of chip is some fixed value, it can
It is uniform to ensure to be formed by microlayer model diameter.
Wherein, the distance of droplet detachment mechanism 200 to 100 outer rim of chip depends on target microlayer model diameter, the first phase liquid
The factors such as 130 property of body, 120 outlet end property of fluid channel, 200 property of droplet detachment mechanism, 100 movement velocity of chip, with true
The droplet that guarantor's fluid channel 120 is formed at 100 outer rim outlet end of chip will not be with core before touching droplet detachment mechanism 200
Piece 100 is detached from.
As further improvement of this embodiment, as shown in figure 3, microlayer model generation device further includes droplet collection device
300, droplet collection device 300 is located at 200 lower section of droplet detachment mechanism, is equipped with two phase liquid in droplet collection device 300
310。
Specifically, working as, drop is bonded in droplet detachment mechanism 200 and chip 100 falls off, after forming microlayer model, due to
The effect of gravity continues to slide from droplet detachment mechanism 200, enters the droplet collection device of 200 lower section of droplet detachment mechanism
In 300.
As chip 100 is persistently driven power effect and relative motion with droplet detachment mechanism 200, microlayer model is de-
After falling, the first phase liquid 130 in liquid storage chamber 110 continues through fluid channel 120 and flows out to 100 outer rim of chip, shown in Fig. 2
Microlayer model generation type continue to repeat, until all first phase liquid 130 form microlayer model, and be collected in droplet collection device
In 300.After completing microlayer model collection, droplet collection device 300 can be taken out, microlayer model is subjected to subsequent operation.
For example, in the field PCR, after completing microlayer model and collecting, entire droplet collection device 300 can be placed in amplification device into
Row amplification, then detects drop, completes digital pcr process.
In this present embodiment, the first phase liquid 130 is water phase, and two phase liquid 310 is oily phase.
Specifically, water phase includes mutually various liquid common in field with oil, wherein water phase includes to be measured in the field PCR
The necessary substance of the digital pcrs such as sample, primer, enzyme.Oil mutually can be the stable oily phase of the various performances based on fluorocarbon oil.According to
Demand can add surfactant in water phase or oily phase, increase the stability of drop with this.
In this present embodiment, chip 100 is disc, and liquid storage chamber 110 is located at 100 central area of chip, droplet detachment machine
For structure 200 around chip 100, corresponding droplet detachment mechanism 200 is the circle concentric with chip 100.Specifically, chip 100 is straight
Diameter is less than 200 diameter of droplet detachment mechanism, wherein the gap of the two radius is 10-30 μm.
In this present embodiment, chip 100 connect (not shown go out) with centrifugation apparatus, centrifugation apparatus be chip 100 provide from
Mental and physical efforts, 100 revolving speed of chip are 200-1000rpm.
In this present embodiment, as shown in figure 4, being provided with the gas vent 140 through 100 thickness direction of chip on chip 100.
Disturbance is mutually generated to the oil stored in droplet collection device 300 when the gas vent 140 is to avoid chip 100 from moving.Due to row
The presence of stomata 140, the negative pressure generated in droplet collection device 300 when chip 100 moves can be by gas vent 140 and outer
Portion is air connected holding air pressure balance, not will drive the movement of oily phase.
The quantity of gas vent 140 is multiple, and 140 shape and size of gas vent are unlimited.
In this present embodiment, the area of gas vent 140 is greater than 1mm2, to avoid 140 area of gas vent is too small and due to hair
The presence of spy influences air communication, to ensure droplet collection device 300 and outside air connection.
It in this present embodiment, is 5-20mm at a distance from chip 100 at the liquid level of two phase liquid.To ensure the second phase liquid
Body 310 is not influenced by the operation of chip 100.
As further improvement of this embodiment, as shown in figure 5, microlayer model generation device further includes outer clamp 400.Outside
Portion's fixture 400 is for fixing drop dropping mechanism 200, to keep droplet detachment mechanism to fix at a distance from 100 outer rim of chip, really
The microlayer model diameter for protecting production is uniform.
Although the embodiments of the present invention has been shown and described above, it is to be understood that above-described embodiment is example
Property, it is not considered as limiting the invention, those skilled in the art within the scope of the invention can be to above-mentioned
Embodiment is changed, modifies, replacement and variant.
Claims (10)
1. a kind of microlayer model generation device, which is characterized in that including chip and droplet detachment mechanism,
It is provided with liquid storage chamber and fluid channel on the chip, is equipped with the first phase liquid, the fluid channel entrance in the liquid storage chamber
End is connected to the liquid storage chamber, and the fluid channel outlet end extends to the outer rim of the chip；
It is opposite that the drop dropping mechanism and the fluid channel are located at one end of the chip outer rim, the drop releasing mechanism and institute
It states and is provided with droplet formation gap between fluid channel outlet end；
The fluid channel outlet end and the contact angle of the first phase liquid are greater than the drop dropping mechanism and the first phase liquid
The contact angle of body.
2. microlayer model generation device according to claim 1, which is characterized in that the chip and the drop dropping mechanism exist
Relative motion is kept during droplet formation.
3. microlayer model generation device according to claim 1, which is characterized in that the fluid channel outlet end and described first
The contact angle of phase liquid is than the drop dropping mechanism and big 10 ° of contact angle or more of the first phase liquid.
4. microlayer model generation device according to claim 3, which is characterized in that the fluid channel outlet end and described first
The contact angle of phase liquid is 20-60 ° bigger than the contact angle of the drop dropping mechanism and the first phase liquid.
5. microlayer model generation device according to claim 1, which is characterized in that the diameter of the fluid channel is 10-50 μm,
The width in the droplet formation gap is 10-30 μm.
6. microlayer model generation device according to claim 1, which is characterized in that the chip is disc, the liquid storage
Chamber is located at the chip center region, and the droplet detachment mechanism is in the chip, the corresponding droplet detachment mechanism
Side is the circle concentric with the chip.
7. microlayer model generation device according to claim 1, which is characterized in that the chip is connect with centrifugation apparatus, institute
It states centrifugation apparatus and provides centrifugal force for the chip, the chip revolving speed is 200-1000rpm.
8. microlayer model generation device according to claim 1, which is characterized in that be provided on the chip through the core
The gas vent of piece thickness direction.
9. microlayer model generation device according to claim 1, which is characterized in that it further include droplet collection device, the liquid
It drips collection device to be located at below the droplet detachment mechanism, is equipped with two phase liquid in the droplet collection device, described second
It is 5-20mm at a distance from the chip at the liquid level of phase liquid.
10. microlayer model generation device according to claim 1, which is characterized in that it further include outer clamp, the external folder
Tool is for fixing the droplet detachment mechanism.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111151314A (en) * | 2020-01-15 | 2020-05-15 | 杭州电子科技大学 | Micro-droplet preparation device and method for ant mouth organ function structure |
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