CN111151314B - Micro-droplet preparation device and method for ant mouth organ function structure - Google Patents
Micro-droplet preparation device and method for ant mouth organ function structure Download PDFInfo
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- CN111151314B CN111151314B CN202010038690.5A CN202010038690A CN111151314B CN 111151314 B CN111151314 B CN 111151314B CN 202010038690 A CN202010038690 A CN 202010038690A CN 111151314 B CN111151314 B CN 111151314B
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- 238000002360 preparation method Methods 0.000 title claims abstract description 22
- 238000000034 method Methods 0.000 title claims abstract description 18
- 239000000835 fiber Substances 0.000 claims abstract description 93
- 239000007788 liquid Substances 0.000 claims abstract description 67
- 230000002209 hydrophobic effect Effects 0.000 claims abstract description 61
- 238000001179 sorption measurement Methods 0.000 claims abstract description 45
- 229920000049 Carbon (fiber) Polymers 0.000 claims description 12
- 239000004917 carbon fiber Substances 0.000 claims description 12
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 claims description 12
- 238000001514 detection method Methods 0.000 claims description 8
- 230000000007 visual effect Effects 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 238000004891 communication Methods 0.000 claims description 3
- 229910001220 stainless steel Inorganic materials 0.000 claims description 3
- 239000010935 stainless steel Substances 0.000 claims description 3
- 210000002050 maxilla Anatomy 0.000 abstract description 6
- 239000002131 composite material Substances 0.000 abstract description 3
- 238000012545 processing Methods 0.000 abstract description 2
- 210000002105 tongue Anatomy 0.000 description 9
- 239000003814 drug Substances 0.000 description 4
- 229940079593 drug Drugs 0.000 description 4
- 238000012827 research and development Methods 0.000 description 4
- 239000011521 glass Substances 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 241000257303 Hymenoptera Species 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 210000004209 hair Anatomy 0.000 description 2
- 238000011179 visual inspection Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 239000004205 dimethyl polysiloxane Substances 0.000 description 1
- 235000013870 dimethyl polysiloxane Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 description 1
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 description 1
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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Abstract
The invention discloses a micro-droplet preparation device and a method for simulating an ant mouth organ functional structure. The existing micro-droplet generation method has the problems that micro-droplets are easy to be lost in a micro-channel and the processing cost of a chip micro-channel is high. The invention relates to a micro-droplet generating device with an ant mouth imitating function structure, which comprises an electric control cabinet, a three-axis driving device, a micro-sample injector, a liquid supporting assembly and an adsorption output assembly. The liquid supporting component comprises an operation workbench, a liquid supporting electric cylinder, a hydrophobic fiber bundle and a first fiber clamp. The adsorption output assembly comprises a double-station electric sliding table, an adsorption station electric cylinder, an output adsorption electric cylinder, a hydrophilic fiber bundle, a second fiber clamp and a hydrophobic slide way. The invention is inspired by the fact that the ant mouthpart composite microstructure (hydrophobic seta + maxilla surface + hydrophilic tongue) has good hydrophobic and hydrophilic characteristics, and the generation of micro-droplets is realized through the motion control of hydrophobic fibers (simulated hydrophobic seta) and hydrophilic fibers (hydrophilic tongue).
Description
Technical Field
The invention belongs to the technical field of micro-droplet precise preparation and control, and particularly relates to a device and a method for preparing micro-droplets simulating an ant mouthpart functional structure.
Background
The micro-droplets are widely applied to the research and development stage of precise mixing of drugs, but the micro-droplets are small, and the difficulty in precise manipulation of the micro-droplets is high, so that how to prepare and manipulate the micro-droplets in a nondestructive and efficient manner becomes a hotspot of technical research. The micro-droplets are widely used in important fields of medical health, biotechnology, drug research and development and the like, particularly in the drug precise mixing research and development stage, however, the micro-droplets are small, and the difficulty in realizing precise manipulation of the micro-droplets is great, so how to prepare and manipulate the micro-droplets in a nondestructive and efficient manner becomes a hotspot of technical research.
At present, the research on the nondestructive and efficient preparation of micro-droplets is very limited, for example, patent publication No. CN110252433A discloses a micro-droplet preparation chip and a manufacturing process method thereof, the chip is composed of a dispersed phase glass capillary, a continuous phase glass capillary, a micro-droplet outlet glass capillary and a PDMS chip, the internal micro-channel structure is a circular T-channel, a continuous phase fluid forms a shear flow in the circular channel, and the shear phase fluid forms micro-droplets, but the preparation method has the problems that the micro-droplets are easily lost in the micro-channel, and the processing cost of the micro-channel of the chip is high.
The ant mouth device structure ants can draw water drops which are several times larger than the body and cannot be submerged by the water drops, and the ants depend on the hydrophobic setae on the surface of the maxilla and the hydrophilic hairs on the surface of the mouth and tongue. As shown in fig. 1 and 2, in fig. 2, a is hydrophobic bristles on the surface of a maxilla, B is a hydrophilic tongue, the hydrophobic bristles fix a liquid drop, and the hydrophilic tongue penetrates into the liquid drop to draw water. The method provides a new idea for realizing the preparation of micro-droplets in a bionic mode.
Disclosure of Invention
The invention aims to provide a micro-droplet preparation device and a method for simulating an ant mouthpart functional structure by simulating a composite micro-structure (hydrophobic seta + maxilla surface + hydrophilic tongue, good hydrophobic and hydrophilic characteristics) of the ant mouthpart aiming at the phenomenon that micro-droplets stick to needles in the existing micro-droplet preparation process of micro-needles. The invention relates to a method for realizing micro-droplet preparation by controlling the motion of hydrophobic fibers (imitating hydrophobic seta) and hydrophilic fibers (hydrophilic tongue); the method is a method for simultaneously clamping and fixing micro-droplets from four directions of a plane and right below the plane through four bundles of hydrophobic fibers and one bundle of hydrophilic fibers; the method is a method for realizing micro-droplet dragging separation and real-time volume metering by real-time precise driving so as to realize the preparation of micro-droplets with customized volumes; the intelligent micro-droplet preparation device integrates micro-needle injection, multi-axis micron-scale precision driving, micro-machine vision and automatic conveying.
The invention relates to a micro-droplet preparation device with an ant mouth imitating function structure, which comprises an electric control cabinet, a three-axis driving device, a micro-sample injector, a liquid supporting assembly and an adsorption output assembly. The three-axis driving device comprises a first electric sliding table, a second electric sliding table and a third electric sliding table. The first electric sliding table is horizontally arranged and is arranged at the top of the electric control cabinet. The second electric sliding table is vertically arranged and is arranged on the sliding block of the first electric sliding table; the third electric sliding table is arranged on the sliding block of the second electric sliding table and is arranged in an inclined mode. The needle cylinder of the microsyringe is arranged on the third electric sliding table, and the push rod head of the microsyringe is driven by the sliding block on the third electric sliding table.
The liquid supporting component comprises an operation workbench, a liquid supporting electric cylinder, a hydrophobic fiber bundle and a first fiber clamp. The control workbench is fixed on the electric control cabinet. The control workbench is provided with a central hole. And the plurality of liquid supporting electric cylinders are all fixed on the control workbench and are arranged along the circumferential direction of the central hole on the control workbench. The push-out rod of each liquid supporting electric cylinder faces to the axis of the central hole. And the pushing rod of each liquid supporting electric cylinder is provided with a hydrophobic fiber bundle. The outer end of each hydrophobic fiber bundle faces to the upper part of the central hole.
The adsorption output assembly comprises a double-station electric sliding table, an adsorption station electric cylinder, an output adsorption electric cylinder, a hydrophilic fiber bundle, a second fiber clamp and a hydrophobic slide way. The double-station electric sliding table is arranged at the top of the electric control cabinet. And an adsorption station electric cylinder and an output adsorption electric cylinder are fixed on a sliding block of the double-station electric sliding table. The ejection rods of the adsorption station electric cylinder and the output adsorption electric cylinder are vertically arranged upwards. And a pushing rod of the electric cylinder of the adsorption station is provided with a hydrophilic fiber bundle. A water drainage slideway is fixed on a push-out rod of the output adsorption electric cylinder. The drainage slideway is obliquely arranged. The top inlet of the hydrophobic slide way and the moving track of the hydrophilic fiber bundle pass through the lower part of the central hole.
Preferably, the hydrophilic fiber bundle has a circumference
F c2 π r γ sin θ - Δ p π r; wherein L isfIs the circumference of the hydrophilic fiber bundle; thetadIs the dynamic contact angle of the hydrophilic fiber bundle and the micro-droplet; gamma rayLVIs a first constant dependent on the type of liquid; γ is a second constant depending on the type of liquid; r is half of liquid bridgeDiameter; θ is the contact angle of the micro-droplet with the tip of the microsyringe; and delta p is the pressure difference between the inside and the outside of the surface of the micro-droplet.
Preferably, the first electric sliding table, the second electric sliding table, the third electric sliding table and the double-station electric sliding table all comprise sliding frames, driving motors, lead screws and sliding blocks. The spindle is mounted on the carriage. The sliding block and the sliding frame form a sliding pair; the nut fixed on the sliding block and the screw rod form a screw pair. The driving motor is fixed on the sliding frame, and the output shaft is fixed with one end of the screw rod.
Preferably, the invention also comprises a visual detection camera and a control system. The visual detection camera is installed on the electric control cabinet and faces the control workbench. The control system is arranged in the electric control cabinet and comprises a touch screen, a motor driver and a controller. The motors in each electric sliding table and each electric cylinder are connected with the controller through motor drivers. And a signal output line of the visual detection camera is connected with a video signal input interface of the controller. The controller adopts PLC. The touch screen is installed on one side lateral wall of automatically controlled cabinet, and communication interface is connected with the controller.
Preferably, the central axis of each hydrophobic fiber bundle is perpendicularly intersected with the axis of the central hole on the operating platform at the same point.
Preferably, the pushing rod of the liquid supporting electric cylinder is fixedly connected with the hydrophobic fiber bundle through a first fiber clamp. And a pushing rod of the adsorption station electric cylinder is fixedly connected with the hydrophilic fiber bundle through a second fiber clamp. The first fiber clamp and the second fiber clamp are both made of 350-mesh stainless steel mesh sheets.
Preferably, the diameter of each of the hydrophobic fiber bundle and the hydrophilic fiber bundle is 10 μm, and the length thereof is 10 mm.
The method for preparing the micro-droplets by using the micro-droplet preparation device with the ant mouth organ simulating function structure comprises the following steps:
step one, filling a volume of target liquid into a microsyringe.
And step two, driving the micro-droplet sample injector to move by the three-axis driving device, so that the needle point of the micro-droplet sample injector reaches a distance d above the intersection point of the central axes of the four hydrophobic carbon fiber bundles.d is the diameter of the microdroplet to be prepared. The volume of the micro-droplets is V1。
Step three, the sliding block of the third electric sliding table pushes the push rod head of the microsyringe to move, so that the needle point of the microsyringe is extruded to be V in volume1The micro-droplets of (a); at this time, the micro-droplet is adhered to the tip of the micro-injector by the liquid bridge force. Meanwhile, the double-station electric sliding table drives the adsorption station electric cylinder to move, so that the hydrophilic fiber bundle reaches the position right below the micro-droplets.
And step four, pushing out each liquid supporting electric cylinder to enable each bundle of hydrophobic carbon fiber bundles to be in contact with the bottom of the micro liquid drop. The hydrophobic carbon fiber bundles generate upward supporting force to the micro-droplets.
Meanwhile, the adsorption station is pushed out by an electric cylinder, so that the top end of the hydrophilic fiber bundle is inserted into the bottom of the micro-droplet; the hydrophilic fiber bundle generates a vertically downward capillary force on the micro-droplets.
And step five, driving the micro sample injector to ascend by the three-axis driving device, so that the micro liquid drops are separated from the needle point of the micro sample injector under the action of the capillary force of the hydrophilic fiber bundle.
And step six, retracting the electric cylinder of the adsorption station to separate the hydrophilic fiber bundles from the micro-droplets.
And step seven, driving the corresponding sliding block to move by the double-station electric sliding table, so that the top inlet of the drainage slideway reaches the position right below the micro liquid drops. And the electric cylinder at the output station is pushed out, so that the distance between the top inlet of the drainage slide way and the micro liquid drops reaches 0.5-3 mm.
And step eight, retracting each liquid supporting electric cylinder to separate each bundle of hydrophobic carbon fiber bundles from the micro liquid drops, and enabling the micro liquid drops to fall to the top inlet of the hydrophobic slide rail and be output along the hydrophobic slide rail.
Preferably, in step four, the hydrophilic fiber bundle is inserted into the depth of the micro-droplet
The invention has the beneficial effects that:
the invention is inspired by the fact that the ant mouthpart composite microstructure (hydrophobic seta + maxilla surface + hydrophilic tongue) has good hydrophobic and hydrophilic characteristics, the micro-droplet preparation is realized through the motion control of hydrophobic fibers (simulated hydrophobic seta) and hydrophilic fibers (hydrophilic tongue), and the micro-droplet is supported and pulled downwards from four directions of a plane and right below the plane through four bundles of hydrophobic fibers and one bundle of hydrophilic fibers, so that the needle sticking and the loss in the micro-droplet preparation process are avoided. In the fields of medical health, biotechnology and drug research and development, the technology plays an important role in precise preparation and transfer of micro-droplets.
Drawings
Fig. 1 is a real image of two maxilla bristles of an ant and their mouth and tongue hairs;
fig. 2 is a diagram of two palatal bristles of an ant and their lips and tongues;
FIG. 3 is a schematic view of the overall structure of the present invention;
FIG. 4 is a schematic view of the combination of a liquid holding assembly and an adsorption output assembly according to the present invention;
FIG. 5 is a schematic diagram of the present invention for preparing microdroplets.
Detailed Description
The invention is further described below with reference to the accompanying drawings.
As shown in fig. 3 and 4, the device for preparing micro-droplets with an ant mouth imitating function structure comprises an electric control cabinet 2, a three-axis driving device, a micro-sampler 8, a liquid holding assembly, an adsorption output assembly, a visual detection camera 12 and a control system. The three-axis driving device comprises a first electric sliding table 3, a second electric sliding table 4 and a third electric sliding table 5. The first electric sliding table 3 is horizontally arranged and is arranged at the top of the electric control cabinet 2. The second electric sliding table 4 is vertically arranged and is arranged on the sliding block of the first electric sliding table 3; the third electric sliding table 5 is installed on the sliding block of the second electric sliding table 4 and is arranged obliquely. The included angle between the guide rail of the third electric sliding table 5 and the horizontal plane can be adjusted. The sample injector push plate 6 is fixed with a sliding block on the third electric sliding table 5; the injector support 7 is fixed to the carriage on the third electric slide table 5. The needle cylinder of the microsyringe 8 is fixed with the injector bracket 7 through an anchor ear, and the push rod head is fixed with the injector push plate 6. When the sliding block of the third electric sliding table 5 slides to the needle cylinder of the microsyringe 8, the sliding block can slideThe micro-sampler 8 can be driven to output micro-droplets. However, the tip of the microsyringe 8 will exert an upward liquid bridge force F on the microdropletscSo that the micro-droplets will be adhered to the tip of the micro-injector 8.
As shown in fig. 4, the pad liquid assembly includes a manipulation stage 17, a pad liquid electric cylinder 9, a hydrophobic fiber bundle 10, and a first fiber holder 18. The operation and control workbench 17 is fixed on the electric control cabinet 2. The operation and control workbench 17 is door-shaped, and the top is provided with a central hole. The four liquid supporting electric cylinders 9 are all fixed on the operation and control workbench 17 and are uniformly distributed along the circumferential direction of a central hole on the operation and control workbench 17. The push-out rods of the four liquid supporting electric cylinders 9 face to the axis of the central hole. First fiber clamps 18 are fixed on the push-out rods of the four liquid supporting electric cylinders 9. The hydrophobic fiber bundle 10 is clamped on the outer side surface of the first fiber clamp 18. The central axes of the four hydrophobic fiber bundles 10 are perpendicularly intersected with the axis of the central hole on the operation and control workbench 17 at the same point. The diameter of the hydrophobic fiber bundle 10 is 10 μm, and the length is 10 mm;
the adsorption output assembly comprises a double-station electric sliding table 16, an adsorption station electric cylinder 15, an output adsorption electric cylinder 14, a hydrophilic fiber bundle 11, a second fiber clamp 19 and a hydrophobic slide rail 13. The double-station electric sliding table 16 is arranged at the top of the electric control cabinet 2; and is located below the top of the manipulation stage 17. And an adsorption station electric cylinder 15 and an output adsorption electric cylinder 14 are fixed on a sliding block of the double-station electric sliding table 16. The ejection rods of the adsorption station electric cylinder 15 and the output adsorption electric cylinder 14 are vertically arranged upwards. A second fiber clamp 19 is fixed on the push-out rod of the adsorption station electric cylinder 15. The top surface of the second fiber holder 19 holds the hydrophilic fiber bundle 11 arranged vertically. The diameter of the hydrophilic fiber bundle 11 is 10 μm, and the length is 10 mm; a water drainage slideway 13 is fixed on a push-out rod of the output adsorption electric cylinder 14. The water drainage slide way 13 is obliquely arranged, and the outlet at the bottom end is connected with subsequent micro-droplet using equipment. The top inlet of the hydrophobic slideway 13 and the moving track of the hydrophilic fiber bundle 11 pass below the intersection point of the central axes of the four hydrophobic fiber bundles 10.
The capillary force F ═ L of the hydrophilic fiber bundle 11 to the micro-dropletsf·γLV·cosθd(ii) a Liquid bridge force F generated by needle tip of microsyringe 8 on micro-droplet c2 π r γ sin θ - Δ p π r; wherein L isfIs the circumference of the hydrophilic fiber bundle; thetadIs the dynamic contact angle of the hydrophilic fiber bundle and the micro-droplet; gamma rayLVIs a first constant dependent on the type of liquid; γ is a second constant depending on the type of liquid; r is the liquid bridge radius; θ is the contact angle of the micro-droplet with the tip of the microsyringe; and delta p is the pressure difference between the inside and the outside of the surface of the micro-droplet. When the capillary force is greater than the liquid bridge force, the hydrophilic fiber bundle 11 can cause the micro-droplets to detach from the micro-injector tip. Therefore, F ≧ FcDetermining the circumference of the hydrophilic fiber bundle 11
The first electric sliding table 3, the second electric sliding table 4, the third electric sliding table 5 and the double-station electric sliding table 16 all comprise a sliding frame, a driving motor, a lead screw and a sliding block. The spindle is mounted on the carriage. The sliding block and the sliding frame form a sliding pair; the nut fixed on the sliding block and the screw rod form a screw pair. The driving motor is fixed on the sliding frame, and the output shaft is fixed with one end of the screw rod.
The control system is arranged in the electric control cabinet and comprises a touch screen 1, a motor driver and a controller. The motors in each electric sliding table and each electric cylinder are connected with the controller through motor drivers. The signal output line of the visual inspection camera 12 is connected with the video signal input interface of the controller. The controller adopts PLC. The touch screen 1 is installed on one side lateral wall of automatically controlled cabinet, and communication interface is connected with the controller.
The method for preparing the micro-droplets by using the micro-droplet preparation device with the ant mouth organ simulating function structure comprises the following steps:
step one, filling a volume V into the micro sample injector 805 mul of target liquid; deionized water is adopted as the target liquid; fixing a push rod head of the microsyringe on the injector push plate 6; the syringe of the microsyringe is fixed to the injector holder 7 by a hoop.
Step (ii) ofAnd secondly, starting a micro-droplet preparation program through the touch screen 1. The first electric sliding table 3 drives the second electric sliding table to move transversely, and the second electric sliding table 4 drives the microsyringe 8 to descend, so that the needle point of the microsyringe 8 reaches a distance d above the intersection point of the central axes of the four bundles of hydrophobic carbon fiber bundles. d is the diameter of the microdroplet to be prepared. The volume of the micro-droplets is V1；V 11 μ L; the diameter d is 0.629 mm.
Step three, the sliding block of the third electric sliding table 5 pushes the push rod head of the microsyringe 8 to move for a preset distance, so that the needle point of the microsyringe is extruded to form a V shape1Micro-droplets of volume, V 11 μ L; at this time, the micro-droplets are adhered to the tip of the micro-injector 8 by the liquid bridge force. Meanwhile, the double-station electric sliding table 16 drives the corresponding sliding block to move, so that the hydrophilic fiber bundle 11 reaches the position right below the micro-droplets 20.
And step four, pushing out the four liquid supporting electric cylinders 9, so that the four hydrophobic carbon fiber bundles 10 are in contact with the bottoms of the micro liquid drops 20. The hydrophobic carbon fiber bundles 10 generate upward supporting force to the micro-droplets 20.
Simultaneously, the adsorption station electric cylinder 15 is pushed out, so that the top end of the hydrophilic fiber bundle 11 is inserted into the bottom of the micro-droplet 20; the hydrophilic fiber bundle 11 is inserted into the micro-droplet 20 to a depth
The hydrophilic fiber bundle 11 generates a vertically downward capillary force on the micro-droplets 20. At this time, the state of the micro-droplets is as shown in fig. 5.
And step five, the second electric sliding table 4 drives the microsyringe 8 to ascend, so that the micro liquid drops 20 are separated from the needle point of the microsyringe under the action of the capillary force of the hydrophilic fiber bundle 11.
And step six, retracting the adsorption station electric cylinder 15 to separate the hydrophilic fiber bundles 11 from the micro-droplets 20.
And step seven, driving the corresponding sliding block to move by the double-station electric sliding table 16, so that the top inlet of the drainage slideway 13 reaches the position right below the micro-droplets 20. And the electric cylinder at the output station is pushed out, so that the distance between the top inlet of the drainage slideway 13 and the micro liquid drops reaches 0.5-3 mm.
And step eight, retracting the four liquid supporting electric cylinders 9 to separate the four hydrophobic carbon fiber bundles 10 from the micro liquid drops 20, and automatically conveying the micro liquid drops to subsequent micro liquid drop using equipment along the hydrophobic slide rail 13 after the micro liquid drops fall to the top inlet of the hydrophobic slide rail 13.
In the first to eighth steps, the visual inspection camera 12 shoots the micro-droplets in real time and transmits the micro-droplets to the controller, so that the controller can monitor the volume and shape changes of the micro-droplets in real time.
Claims (8)
1. A micro-droplet preparation device with an ant mouth-piece simulating functional structure comprises an electric control cabinet, a three-axis driving device, a micro-sample injector, a liquid supporting assembly and an adsorption output assembly; the method is characterized in that: the three-axis driving device comprises a first electric sliding table, a second electric sliding table and a third electric sliding table; the first electric sliding table is horizontally arranged and is arranged at the top of the electric control cabinet; the second electric sliding table is vertically arranged and is arranged on the sliding block of the first electric sliding table; the third electric sliding table is arranged on the sliding block of the second electric sliding table and is obliquely arranged; a needle cylinder of the microsyringe is arranged on the third electric sliding table, and a push rod head of the microsyringe is driven by a sliding block on the third electric sliding table;
the liquid supporting component comprises an operation workbench, a liquid supporting electric cylinder, a hydrophobic fiber bundle and a first fiber clamp; the control workbench is fixed on the electric control cabinet; a central hole is formed in the control workbench; the plurality of liquid supporting electric cylinders are all fixed on the control workbench and are arranged along the circumferential direction of a central hole on the control workbench; the pushing rod of each liquid supporting electric cylinder faces to the axis of the central hole; the pushing rod of each liquid supporting electric cylinder is provided with a hydrophobic fiber bundle; the outer end of each hydrophobic fiber bundle faces the upper part of the central hole;
the adsorption output assembly comprises a double-station electric sliding table, an adsorption station electric cylinder, an output adsorption electric cylinder, a hydrophilic fiber bundle, a second fiber clamp and a hydrophobic slideway; the double-station electric sliding table is arranged at the top of the electric control cabinet; an adsorption station electric cylinder and an output adsorption electric cylinder are fixed on a sliding block of the double-station electric sliding table; the adsorption station electric cylinder and the push-out rod of the output adsorption electric cylinder are vertically arranged upwards; a pushing rod of the adsorption station electric cylinder is provided with a hydrophilic fiber bundle; a water drainage slideway is fixed on a push-out rod of the output adsorption electric cylinder; the drainage slideway is obliquely arranged; the top inlet of the hydrophobic slide way and the moving track of the hydrophilic fiber bundle pass through the lower part of the central hole.
2. The device for preparing micro-droplets simulating the functional structure of an ant mouth organ as claimed in claim 1, wherein: the first electric sliding table, the second electric sliding table, the third electric sliding table and the double-station electric sliding table respectively comprise a sliding frame, a driving motor, a lead screw and a sliding block; the lead screw is supported on the sliding frame; the sliding block and the sliding frame form a sliding pair; the nut fixed on the sliding block and the screw rod form a screw pair; the driving motor is fixed on the sliding frame, and the output shaft is fixed with one end of the screw rod.
3. The device for preparing micro-droplets simulating the functional structure of an ant mouth organ as claimed in claim 1, wherein: the system also comprises a visual detection camera and a control system; the visual detection camera is arranged on the electric control cabinet and faces the control workbench; the control system is arranged in the electric control cabinet and comprises a touch screen, a motor driver and a controller; the motors in the electric sliding tables and the electric cylinders are connected with a controller through motor drivers; a signal output line of the visual detection camera is connected with a video signal input interface of the controller; the controller adopts a PLC; the touch screen is installed on one side lateral wall of automatically controlled cabinet, and communication interface is connected with the controller.
4. The device for preparing micro-droplets simulating the functional structure of an ant mouth organ as claimed in claim 1, wherein: the central axis of each hydrophobic fiber bundle is vertically intersected with the axis of the central hole on the control workbench at the same point.
5. The device for preparing micro-droplets simulating the functional structure of an ant mouth organ as claimed in claim 1, wherein: the pushing rod of the liquid supporting electric cylinder is fixedly connected with the hydrophobic fiber bundle through a first fiber clamp; a pushing rod of the adsorption station electric cylinder is fixedly connected with the hydrophilic fiber bundle through a second fiber clamp; the first fiber clamp and the second fiber clamp are both made of 350-mesh stainless steel mesh sheets.
6. The device for preparing micro-droplets simulating the functional structure of an ant mouth organ as claimed in claim 1, wherein: the diameter of the hydrophobic fiber bundle and the length of the hydrophilic fiber bundle are both 10 micrometers, and the length of the hydrophobic fiber bundle and the length of the hydrophilic fiber bundle are both 10 mm.
7. The method for preparing micro-droplets by using the micro-droplet preparation device with the ant mouth organ imitating function structure as claimed in claim 1, is characterized in that: step one, pouring target liquid into a micro sample injector;
driving the micro-droplet sample injector to move by the three-axis driving device, so that the needle point of the micro-droplet sample injector reaches a distance d above the intersection point of the central axes of the four hydrophobic carbon fiber bundles; d is the diameter of the micro-droplet to be prepared; the volume of the micro-droplets is V1；
Step three, the sliding block of the third electric sliding table pushes the push rod head of the microsyringe to move, so that the needle point of the microsyringe is extruded to be V in volume1The micro-droplets of (a); at the moment, the micro-liquid drop is adhered to the needle tip of the microsyringe under the action of the liquid bridge force; meanwhile, the double-station electric sliding table drives the adsorption station electric cylinder to move, so that the hydrophilic fiber bundle reaches the position right below the micro-droplets;
pushing out each liquid supporting electric cylinder to enable each bundle of hydrophobic carbon fiber bundles to be in contact with the bottom of the micro-droplet; the hydrophobic carbon fiber bundle generates upward supporting force on the micro-droplets;
meanwhile, the adsorption station is pushed out by an electric cylinder, so that the top end of the hydrophilic fiber bundle is inserted into the bottom of the micro-droplet; the hydrophilic fiber bundle generates vertically downward capillary force on the micro-droplets;
fifthly, the triaxial driving device drives the microsyringe to rise, so that the micro liquid drops are separated from the needle tip of the microsyringe under the action of the capillary force of the hydrophilic fiber bundle;
step six, retracting the electric cylinder of the adsorption station to separate the hydrophilic fiber bundles from the micro-droplets;
step seven, the double-station electric sliding table drives the corresponding sliding block to move, so that the top inlet of the drainage slideway reaches the position right below the micro liquid drops; and the electric cylinder at the output station is pushed out, so that the distance between the top inlet of the drainage slide way and the micro liquid drops reaches 0.5-3 mm;
and step eight, retracting each liquid supporting electric cylinder to separate each bundle of hydrophobic carbon fiber bundles from the micro liquid drops, and enabling the micro liquid drops to fall to the top inlet of the hydrophobic slide rail and be output along the hydrophobic slide rail.
8. The method for preparing micro-droplets by the micro-droplet preparation device with the ant mouth imitating functional structure as claimed in claim 7, wherein: in the fourth step, the hydrophilic fiber bundle is inserted into the depth of the micro-droplet
Priority Applications (1)
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