CN109971830B - Simple experimental device for DNA spot hybridization - Google Patents

Abstract

The invention discloses a simple experimental device for DNA spot hybridization, which comprises a bearing chassis, wherein one side of the upper end of the bearing chassis is vertically and fixedly connected with a vertical rod, the upper end of the vertical rod is sequentially and movably connected with a first telescopic bracket, a second telescopic bracket and a third telescopic bracket from bottom to top, one end of the third telescopic bracket is connected with a microsyringe needle fixing bracket, two sides of one end surface of the microsyringe needle fixing bracket are uniformly provided with a plurality of mutually corresponding elastic limiting mechanisms, microsyringes needles are arranged between the elastic limiting mechanisms on two sides, one end of the second bracket is connected with an operation table below the microsyringe needle fixing bracket, the upper end surface of the operation table is provided with a nylon membrane, and one side above the bearing chassis is provided with a negative pressure aspirator, and the experimental device has the beneficial effects that: the device has no corresponding limitation on the loading quantity, is simple and easy to operate according to the determination required by experiments, can improve the uncertainty of sample combination caused in the process of natural air drying in manual loading by combining the negative pressure suction device, and saves the time cost.

Description

A simple experimental device for DNA dot hybridization

technical field

The invention belongs to the technical field of DNA dot hybridization, in particular to a simple experimental device for DNA dot hybridization.

Background technique

DNA dot hybridization technology is a simple experimental technique to combine the DNA samples to be tested on nitrocellulose or nylon membranes, prepare for subsequent hybridization experiments, and judge whether there is hybridization and its hybridization intensity through autoradiography. It can be done by manual spotting or dot hybridization: manual spotting means directly pointing the sample on the membrane with a pipette gun and waiting for the sample to dry naturally, so natural air drying in manual spotting will cause instability of the experimental results and increase the experimental time . The number of sample wells in a commercial dot blot hybridization instrument is limited by the instrument to 48 or 96 wells. When the number of samples to be loaded in the experiment does not meet the number of sample spaces, the cost of the experiment will be greatly increased. At the same time, commercial dot hybridization instruments are expensive and costly.

Contents of the invention

The object of the present invention is to provide a simple experimental device for DNA dot hybridization in order to solve the above problems, which solves the current existing problems.

In order to solve the above problems, the present invention provides a technical solution:

A simple experimental device for DNA dot hybridization, comprising a load-bearing chassis, one side of the upper end of the load-bearing chassis is vertically fixedly connected with a vertical rod, and the upper end of the vertical rod is connected to the No. support and the No. 3 telescopic support, bearings are connected between the No. 3 telescopic support and the No. 1 telescopic support and the vertical bar, and a micro-injection needle fixing frame is connected to one end of the No. 3 telescopic support, and the micro-injection needle On both sides of one end face of the fixed frame, a number of elastic limit mechanisms corresponding to each other are evenly arranged, and a micro-injection needle is arranged between the elastic limit mechanisms on both sides, and one end of the No. 2 bracket is fixed on the micro-injection needle. The bottom of the frame is connected with an operation table, the upper end of the operation table is provided with a nylon film, and a negative pressure suction device is provided on the upper side of the load-bearing chassis, and a negative pressure controller is provided on the negative pressure suction device. The output end of the suction device is connected with the suction head of the negative pressure suction device through the No. 1 telescopic bracket, and the suction head of the negative pressure suction device is movably connected with the nylon membrane through the No. 1 telescopic bracket.

As preferably, the elastic limit mechanism is composed of a fixed block, a limit rod, an abutment plate, a rubber pad, a slot, a slider, a chute, a spring pad and a telescopic spring, and the fixed block and the micro-sampling needle holder It is fixedly connected and located on both sides of the micro-sampling needle. There are two parallel slots in the fixed block. A number of sliders are evenly connected to the upper and lower walls of the slot. The movable insertion in the slot is limited. Position rod, the upper and lower ends of the limit rod are provided with chute, the chute is connected with the slider, the limit rod is slidably connected with the chute through the slider, the bottom end of the slot is connected with the limit A spring pad is provided at the end of the positioning rod away from the micro-injection needle, and a telescopic spring is connected between the two spring pads.

Preferably, filter paper is laid on the bottom surface of the nylon membrane.

Preferably, the top end of the suction head of the negative pressure suction device is under the nylon membrane.

Beneficial effects of the present invention:

1. The number of loading holes of the dot hybridization instrument is limited by the instrument to 48 or 96 holes. When the actual number of samples in the experiment is not satisfied, the cost of the experiment will be increased. The number of samples loaded by this device has no corresponding limit. According to the experimental results It needs to be decided, and the operation is simple and easy.

2. The negative pressure suction device used in this device can improve the uncertainty of sample combination caused by the natural air-drying process in manual sample loading, and save time and cost at the same time.

3. Compared with multi-tube blotting instruments and hybridization instruments sold in the market, the cost of this device is lower.

Description of drawings:

For ease of illustration, the present invention is described in detail by the following specific implementations and accompanying drawings.

Fig. 1 is a structural representation of the present invention;

Fig. 2 is a structural schematic diagram of the elastic limit mechanism of the present invention;

Fig. 3 is a schematic diagram of the enlarged structure at A of the present invention.

In the figure: 1. Load-bearing chassis; 2. Vertical rod; 3. No. 1 telescopic support; 4. No. 2 support; 5. No. 3 telescopic support; 6. Bearing; Position mechanism; 81, fixed block; 82, limit rod; 83, arrival plate; 84, rubber pad; 85, slot; 86, slider; 87, chute; 88, spring pad; 89, telescopic spring; 9 10. Operating table; 11. Nylon membrane; 12. Negative pressure aspirator; 13. Negative pressure controller; 14. Negative pressure aspirator tip.

Detailed ways:

As shown in Figures 1-3, the present embodiment adopts the following technical scheme: a simple experimental device for DNA dot hybridization, comprising a load-bearing chassis 1, the upper end of the load-bearing chassis 1 is vertically fixedly connected with a vertical rod 2 , the upper end of the vertical rod 2 is movably connected to No. 1 telescopic bracket 3, No. 2 bracket 4 and No. 3 telescopic bracket 5 from bottom to top, and one end of the No. 3 telescopic bracket 5 is connected to a micro-injection needle holder 7, so On both sides of the end surface of the micro-sampling needle holder 7, a number of corresponding elastic stoppers 8 are evenly arranged, and a micro-sampling needle 9 is arranged between the elastic stoppers 8 on both sides. One end of the support 4 is connected with an operation table 10 below the micro-sampling needle holder 7, the upper end of the operation table 10 is provided with a nylon membrane 11, and the upper side of the load-bearing chassis 1 is provided with a negative pressure suction device 12. The negative pressure suction device 12 is provided with a negative pressure controller 13, and the output end of the negative pressure suction device 12 passes through the No. 1 telescopic support 3 and is connected with a negative pressure suction device suction head 14, and the negative pressure suction device suction head 14 Via the No. 1 telescopic bracket 3, it is flexibly connected with the nylon membrane 11.

Wherein, the elastic limit mechanism 8 is composed of a fixed block 81, a limit rod 82, an abutment plate 83, a rubber pad 84, a slot 85, a slider 86, a chute 87, a spring pad 88 and a telescopic spring 89. The fixed block 81 is fixedly connected with the micro-sampling needle holder 7 and is located on both sides of the micro-sampling needle 9. The fixed block 81 is provided with two parallel slots 85, and the upper and lower walls of the slots 85 are evenly spaced. A plurality of slide blocks 86 are connected, and a limit rod 82 is movably inserted in the slot 85. A chute 87 is arranged on the upper and lower ends of the limit rod 82, and the chute 87 is connected with the slide block 86. The stop bar 82 is slidably connected with the chute 87 through the slider 86, and the inner bottom end of the slot 85 and the end of the stop bar 82 away from the micro-sampling needle 9 are all provided with a spring pad 88, and the two springs A telescopic spring 89 is connected between the pads 88 .

Wherein, the bottom surface of the nylon membrane 11 is covered with filter paper to increase the filtration efficiency of the solvent.

Wherein, the top of the suction head 14 of the negative pressure suction device is located below the nylon membrane 11. With the cooperation of the No. 1 telescopic bracket 3 and the bearing 6, the suction head of the negative pressure suction device will move position along with the change of the spotting position. The negative pressure aspirator 12 simulates a vacuum environment, so that the DNA in the sample can be combined with the nylon membrane 11 faster and better.

Wherein, a bearing 6 is connected between the No. 3 telescopic support 5 and the No. 1 telescopic support 3 and the vertical bar 2. Cooperating with the No. 1 telescopic support 3, the injection angle of the micro-sampling needle 9 can be adjusted in all directions, and the direct simulation The micro-sampling needle 9 manually adds samples.

Specifically: when the present invention is in use, select a micro-injection needle 9 with a suitable volume (such as 10 μl, 20 μl, 100 μl) according to the volume of the sample, and place the micro-injection needle 9 on the elastic limit mechanism on both sides after sucking the sample 8, the micro-sampling needle 9 is clamped and fixed on the micro-sampling needle holder 7, and the suction head 14 of the micro-sampling needle holder 7, the nylon membrane 11, and the negative pressure suction device 12 are adjusted to determine In the area of the sample on the nylon membrane 11, the volume of the sample is manually controlled, and the suction strength of the negative pressure aspirator 12 is adjusted through the negative pressure controller 13 to simulate a vacuum environment, so that the DNA in the sample can be removed faster and better. Combined with the nylon membrane 11, the solvent is taken away by the negative pressure suction device 12.

The control mode of the present invention is automatically controlled by a negative pressure controller, and the control circuit of the negative pressure controller can be realized through simple programming by those skilled in the art. The supply of power also belongs to the common knowledge in this field, and the present invention mainly uses To protect the mechanical device, so the present invention no longer explains the control mode and circuit connection in detail.

Above shows and described basic principle of the present invention and main feature and the advantage of the present invention, those skilled in the art should understand that, the present invention is not limited by above-mentioned embodiment, and what described in above-mentioned embodiment and description just illustrates the present invention Principle, under the premise of not departing from the spirit and scope of the present invention, the present invention also has various changes and improvements, and these changes and improvements all fall within the claimed scope of the present invention, and the claimed scope of the present invention is defined by the appended claims Requirements and their equivalents are defined.

Claims (3)

1. a kind of simple and easy experiment device that is used for DNA spot hybridization, comprises load-bearing chassis (1), it is characterized in that: described load-bearing chassis (1) upper end one side is vertically fixedly connected with vertical bar (2), and described vertical bar (2) The upper end is movably connected to No. 1 telescopic bracket (3), No. 2 bracket (4) and No. 3 telescopic bracket (5) sequentially from bottom to top. The No. 3 telescopic bracket (5) and No. 1 telescopic bracket (3) ) and the vertical bar (2) are connected with a bearing (6), and one end of the No. A number of corresponding elastic limit mechanisms (8) are evenly arranged on both sides of one end surface, and micro-sampling needles (9) are arranged between the elastic limit mechanisms (8) on both sides, and the No. 2 bracket ( 4) One end is connected with an operation platform (10) under the micro-sampling needle fixing frame (7). The upper surface of the operation platform (10) is provided with a nylon membrane (11), and the upper side of the load-bearing chassis (1) is A negative pressure suction device (12) is provided, and a negative pressure controller (13) is provided on the negative pressure suction device (12), and the output end of the negative pressure suction device (12) passes through the No. 1 telescopic bracket (3 ) is connected with the vacuum suction device tip (14), and the negative pressure suction device suction tip (14) is flexibly connected with the nylon membrane (11) through the No. 1 telescopic bracket (3), and the negative pressure suction device suction tip ( 14) The top is under the nylon membrane (11). 2. A simple experimental device for DNA dot hybridization according to claim 1, characterized in that: the elastic limit mechanism (8) consists of a fixed block (81), a limit rod (82), a stop plate (83), rubber pad (84), slot (85), slide block (86), chute (87), spring pad (88) and telescopic spring (89), the fixed block (81) and The micro-sampling needle holder (7) is fixedly connected and is on both sides of the micro-sampling needle (9), and the fixed block (81) is provided with two parallel slots (85), and the slots (85 ) are evenly connected with some sliders (86) on the upper and lower walls, and a limit rod (82) is movably inserted in the slot (85), and a chute (82) is provided at the upper and lower ends of the limit rod (82) 87), the chute (87) is connected with the slide block (86), the limit rod (82) is slidably connected with the chute (87) through the slide block (86), and the inside of the slot (85) A spring pad (88) is provided at the bottom end and the end of the limit rod (82) away from the micro-sampling needle (9), and a telescopic spring (89) is connected between the two spring pads (88). 3. A simple experimental device for DNA dot hybridization according to claim 1, characterized in that: the bottom surface of the nylon membrane (11) is covered with filter paper.