CN109971690A - A kind of pool mouth spreads cultivation the culture medium and propagation method of lactic acid bacteria - Google Patents
A kind of pool mouth spreads cultivation the culture medium and propagation method of lactic acid bacteria Download PDFInfo
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- CN109971690A CN109971690A CN201910380208.3A CN201910380208A CN109971690A CN 109971690 A CN109971690 A CN 109971690A CN 201910380208 A CN201910380208 A CN 201910380208A CN 109971690 A CN109971690 A CN 109971690A
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Abstract
It spreads cultivation the culture medium and propagation method of lactic acid bacteria the present invention relates to a kind of pool mouth, belongs to technical field of aquaculture.Take glucose, potassium dihydrogen phosphate, wheat bran, ammonium chloride, potassium chloride, magnesium sulfate, sodium chloride；Above-mentioned raw materials are stirred at normal temperature and are spread cultivation the culture medium of lactic acid bacteria to get pool mouth.Empty barrel with cover is taken, tap water is filled, gas explosion removes chlorine residue；It takes mouth the spread cultivation culture medium of lactic acid bacteria in the pool to be added in the quick-fried water for crossing gas, adds and need the lactic acid bacteria that spreads cultivation, be uniformly mixing to obtain mixed bacteria liquid, cover bung；Mixed bacteria liquid is placed under sunlight and is fermented, rocks to obtain pool mouth in fermentation process daily and spreads cultivation lactic acid bacteria.Culture medium of the present invention is suitble to lactobacter growth demand, lactic acid bacteria can be promoted to mushroom out.Propagation method is simple, can reduce bringing into for external source miscellaneous bacteria using the tap water of gas explosion, keeps anaerobic state that can effectively inhibit varied bacteria growing during spreading cultivation, improves the success rate that spreads cultivation.
Description
Technical field
It spreads cultivation the culture medium and propagation method of lactic acid bacteria the present invention relates to a kind of pool mouth, belongs to technical field of aquaculture.
Background technique
In recent years, excessive use of broad-spectrum antibiotic drug not only increases the drug resistance of pathogen, but also to aquatic products
Safety constitute a threat to.Microorganism formulation have prevent disease, enhancing immunity of organisms, promote grow, put on weight etc. it is a variety of
Function, and pollution-free, noresidue, drug resistance is not generated, to become ideal one of the substitute of antibiotic.Lactic acid bacteria is
More common one kind in probiotics, lactic acid bacteria is chiefly used in adjusting breeding environment in the breeding productions such as prawn, fish at present,
Cultivated water environment can not only be improved, the immunity of organisms of the cultivation object such as fish, shrimp can also be improved, reduce the hair of disease
It is raw.But lactic acid bacteria presses normal dosage in use, uses 1kg per acre, works slower, if increasing dosage, raiser
Use cost be significantly increased.There is part raiser to spread cultivation using fermentor at present, but fermentor is at high cost, additionally need compared with
High technical level is not suitable for raiser and generally uses.Also part raiser is spread cultivation microorganism with easy bucket and air pump, but
In the case where ventilation, miscellaneous bacteria is easy to grow, and causes to spread cultivation unsuccessfully.
Summary of the invention
It is an object of the present invention to overcome the above deficiencies, facilitates raiser can be on the pool mouthful to lactic acid bacteria to provide
Spread cultivation, raising spreads cultivation success rate, reduce raiser's use cost a kind of pool mouth spread cultivation lactic acid bacteria culture medium and spread cultivation
Method.
According to technical solution provided by the invention, a kind of pool mouth spreads cultivation the culture medium of lactic acid bacteria, includes following component, matches
Square ratio by mass number meter: taking 40-60 parts of glucose, 5-15 parts of potassium dihydrogen phosphate, wheat bran is 1-10 parts, ammonium chloride 5-20
Part, 5-10 parts of potassium chloride, 5-10 parts of magnesium sulfate, 5-10 parts of sodium chloride；Above-mentioned raw materials are stirred through trough type mixing machine at normal temperature
Mixing 30-80 minute, 20-70 revs/min of revolving speed, be packaged into 1kg/ bag to obtain the final product the pool mouthful spread cultivation the culture medium of lactic acid bacteria.
As a further improvement of the present invention, formula rate is according to parts by weight: taking 40 parts of glucose, potassium dihydrogen phosphate
15 parts, wheat bran be 10 parts, 20 parts of ammonium chloride, 5 parts of potassium chloride, 5 parts of magnesium sulfate, 5 parts of sodium chloride；Above-mentioned raw materials are passed through at normal temperature
Trough type mixing machine is stirred 40 minutes, 30 revs/min of revolving speed, is packaged into 1kg/ bags and is spread cultivation the culture of lactic acid bacteria up to pool mouth
Base.
As a further improvement of the present invention, formula rate is according to parts by weight: taking 45 parts of glucose, potassium dihydrogen phosphate
10 parts, wheat bran be 8 parts, 17 parts of ammonium chloride, 6 parts of potassium chloride, 7 parts of magnesium sulfate, 7 parts of sodium chloride；Above-mentioned raw materials are passed through at normal temperature
Trough type mixing machine is stirred 50 minutes, 45 revs/min of revolving speed, is packaged into 1kg/ bags and is spread cultivation the culture of lactic acid bacteria up to pool mouth
Base.
As a further improvement of the present invention, formula rate is according to parts by weight: taking 50 parts of glucose, potassium dihydrogen phosphate
12 parts, wheat bran be 6 parts, 10 parts of ammonium chloride, 10 parts of potassium chloride, 8 parts of magnesium sulfate, 4 parts of sodium chloride；Above-mentioned raw materials are passed through at normal temperature
Trough type mixing machine is stirred 60 minutes, 55 revs/min of revolving speed, is packaged into 1kg/ bags and is spread cultivation the culture of lactic acid bacteria up to pool mouth
Base.
As a further improvement of the present invention, a kind of pool mouth spreads cultivation the propagation method of lactic acid bacteria, it is characterized in that: using following
Processing step:
Step 1, preparation of culture medium: its formula rate is according to parts by weight: taking 40-60 parts of glucose, potassium dihydrogen phosphate 5-15
Part, wheat bran are 1-10 parts, 5-20 parts of ammonium chloride, 5-10 parts of potassium chloride, 5-10 parts of magnesium sulfate, 5-10 parts of sodium chloride；By above-mentioned original
Material is stirred 30-80 minutes through trough type mixing machine at normal temperature, 20-70 revs/min of revolving speed, is packaged into 1kg/ bags up to the pool mouthful
Spread cultivation the culture medium of lactic acid bacteria, spare；
Step 2 removes chlorine residue: taking the empty barrel with cover of 50-100L, fills tap water, and gas explosion 24-36h removes chlorine residue；
Step 3, mixed bacteria liquid preparation: take the pool mouth spread cultivation lactic acid bacteria culture medium 1-3kg be added to it is quick-fried cross gas water in, then plus
Enter the lactic acid bacteria 1-3kg for needing to spread cultivation, is stirred 5-10 minutes with 10-20 revs/min of speed and obtain mixed bacteria liquid, cover bucket
Lid；
Fermentation: step 4 mixed bacteria liquid obtained in step 3 is placed in the sun, is issued in 15-25 DEG C of temperature condition
It ferment 4-6 days, is rocked in fermentation process daily and obtains pool mouth for 1-3 times and spread cultivation lactic acid bacteria.
As a further improvement of the present invention, mixed bacteria liquid obtained in step 3 is placed in the cool, fermentation temperature
It 26-35 DEG C, ferments 2-4 days, is rocked in fermentation process daily and obtain the pool mouthful for 1-3 times and spread cultivation lactic acid bacteria.
Compared with the prior art the present invention has the advantage that
Culture medium of the present invention is suitble to lactobacter growth demand, lactic acid bacteria can be promoted to mushroom out.Propagation method is simple, and use is quick-fried
The tap water of gas can reduce bringing into for external source miscellaneous bacteria, keep anaerobic state that can effectively inhibit varied bacteria growing during spreading cultivation,
Raising spreads cultivation success rate.
Specific embodiment
The present invention will be further described in conjunction with the embodiments below:
Embodiment 1: a kind of pool of present invention mouth spreads cultivation the culture medium and propagation method of lactic acid bacteria, and using following processing step: it is matched
Square ratio by mass number meter:
Step 1, preparation of culture medium: taking 40 parts of glucose, 15 parts of potassium dihydrogen phosphate, wheat bran is 10 parts, 20 parts of ammonium chloride, chlorination
5 parts of potassium, 5 parts of magnesium sulfate, 5 parts of sodium chloride；Above-mentioned raw materials are stirred 40 minutes through trough type mixing machine at normal temperature, revolving speed 30
Rev/min, it is packaged into 1kg/ bags and spreads cultivation the culture medium of lactic acid bacteria up to pool mouth, it is spare；
Step 2 removes chlorine residue: taking the empty barrel with cover of 50L, fill tap water, gas explosion removes chlorine residue for 24 hours.
Mixed bacteria liquid preparation: step 3 above-mentioned culture medium 1kg is added in the quick-fried water for crossing gas, needs is added and spreads cultivation
Lactic acid bacteria 1kg, stirred 10 minutes with 10 revs/min of speed and obtain mixed bacteria liquid, cover bung；
Fermentation: step 4 places in the sun, issues ferment 5 days in 20 DEG C of temperature conditions, rock daily in fermentation process 3 times；
It 30 DEG C of fermentation temperature, ferments 3 days, placement is rocked daily in the cool, in fermentation process to be obtained pool mouth for 3 times and spread cultivation lactic acid bacteria.
The function of each component is as follows in the present invention:
1, glucose: organic compound, molecular formula C6H12O6, it is a kind of most wide and mostly important monosaccharide of distributed in nature, it can
Carbon source is provided for the growth of lactic acid bacteria.
2, potassium dihydrogen phosphate: chemical formula KH2PO4, solution agglomeration easy to damp, rich in P elements and potassium element, promotion lactic acid bacteria is raw
It is long.
3, wheat bran: the byproduct after wheat processing starch can keep material after mixing with the material of other solution agglomerations easy to damp
Loosely, it prevents from agglomerating.
4, ammonium chloride: the byproduct of basic industry is rich in nitrogen, can provide nitrogen source for the growth of lactic acid bacteria.
5, potassium chloride: it is soluble easily in water, the raising of solubility with temperature in water and promptly increase, be rich in potassium element.
6, a kind of magnesium sulfate: soluble easily in water, compound containing magnesium.
7, sodium chloride: the main component of salt, stability are good.
8, the lactic acid bacteria: lactobacillus acidophilus, bacterium numbering AS1.2686.
Embodiment 2: a kind of pool of present invention mouth spreads cultivation the culture medium and propagation method of lactic acid bacteria, using following processing step:
Its formula rate is according to parts by weight:
Step 1, preparation of culture medium: taking 45 parts of glucose, 10 parts of potassium dihydrogen phosphate, wheat bran is 8 parts, 17 parts of ammonium chloride, potassium chloride
6 parts, 7 parts of magnesium sulfate, 7 parts of sodium chloride；Above-mentioned raw materials are stirred 50 minutes through trough type mixing machine at normal temperature, revolving speed 45
Rev/min, it is packaged into 1kg/ bags and spreads cultivation the culture medium of lactic acid bacteria up to pool mouth, it is spare；
Step 2 removes chlorine residue: taking the empty barrel with cover of 80L, fills tap water, and gas explosion 30h removes chlorine residue.
Mixed bacteria liquid preparation: step 3 above-mentioned culture medium 2kg is added in the quick-fried water for crossing gas, needs is added and spreads cultivation
Lactic acid bacteria 1.5kg, stirred 8 minutes with 15 revs/min of speed and obtain mixed bacteria liquid, cover bung；
Fermentation: step 4 24 DEG C of fermentation temperature, places in the sun, ferments 4 days, rock 2 times daily in fermentation process and obtain the pool
Mouth spreads cultivation lactic acid bacteria.
Embodiment 3: a kind of pool of present invention mouth spreads cultivation the culture medium and propagation method of lactic acid bacteria, using following processing step:
Its formula rate is according to parts by weight:
Step 1, preparation of culture medium: taking 50 parts of glucose, 12 parts of potassium dihydrogen phosphate, wheat bran is 6 parts, 10 parts of ammonium chloride, potassium chloride
10 parts, 8 parts of magnesium sulfate, 4 parts of sodium chloride；Above-mentioned raw materials are stirred 60 minutes through trough type mixing machine at normal temperature, revolving speed 55
Rev/min, it is packaged into 1kg/ bags and spreads cultivation the culture medium of lactic acid bacteria up to pool mouth, it is spare；
Step 2 removes chlorine residue: taking the empty barrel with cover of 100L, fills tap water, and gas explosion 32h removes chlorine residue.
Mixed bacteria liquid preparation: step 3 above-mentioned culture medium 3kg is added in the quick-fried water for crossing gas, needs is added and spreads cultivation
Lactic acid bacteria 2kg, stirred 6 minutes with 18 revs/min of speed and obtain mixed bacteria liquid, cover bung；
Fermentation: step 4 places in the sun, issues ferment 3 days in 28 DEG C of temperature conditions, rock daily in fermentation process 1 time
Pool mouth is obtained to spread cultivation lactic acid bacteria.
Using effect of the invention is as follows:
It is used by the river crab cultivation pond of Suzhou, 1kg culture medium is added in the quick-fried water for crossing gas, lactic acid bacteria 1kg is added,
It is stirred 8 minutes with 15 revs/min of speed, covers bung, placed in the sun, issue ferment 4 days in 25 DEG C of temperature conditions, expanded
50kg bacterium solution is trained out, microscopy lactobacter growth is good, and spread cultivation success, greatly reduces the use cost of raiser.
Claims (6)
- The culture medium of lactic acid bacteria 1. a kind of pool mouth spreads cultivation, it is characterized in that: formula rate is in parts by weight comprising following component Meter: taking 40-60 parts of glucose, 5-15 parts of potassium dihydrogen phosphate, wheat bran is 1-10 parts, 5-20 parts of ammonium chloride, 5-10 parts of potassium chloride, sulphur Sour magnesium 5-10 parts, 5-10 parts of sodium chloride；Above-mentioned raw materials are stirred 30-80 minutes through trough type mixing machine at normal temperature, revolving speed It 20-70 revs/min, is packaged into 1kg/ bags and spreads cultivation the culture medium of lactic acid bacteria to get pool mouth.
- The culture medium of lactic acid bacteria 2. a kind of pool mouth as described in claim 1 spreads cultivation, it is characterized in that: its formula rate is by weight Number meter: taking 40 parts of glucose, 15 parts of potassium dihydrogen phosphate, wheat bran is 10 parts, 20 parts of ammonium chloride, 5 parts of potassium chloride, 5 parts of magnesium sulfate, chlorine Change 5 parts of sodium；Above-mentioned raw materials are stirred at normal temperature, 1kg/ bags is packaged into and spreads cultivation the culture medium of lactic acid bacteria to get pool mouth.
- The culture medium of lactic acid bacteria 3. a kind of pool mouth as described in claim 1 spreads cultivation, it is characterized in that: its formula rate is by weight Number meter: taking 45 parts of glucose, 10 parts of potassium dihydrogen phosphate, wheat bran is 8 parts, 17 parts of ammonium chloride, 6 parts of potassium chloride, 7 parts of magnesium sulfate, chlorine Change 7 parts of sodium；Above-mentioned raw materials are stirred at normal temperature, 1kg/ bags is packaged into and spreads cultivation the culture medium of lactic acid bacteria to get pool mouth.
- The culture medium of lactic acid bacteria 4. a kind of pool mouth as described in claim 1 spreads cultivation, it is characterized in that: its formula rate is by weight Number meter: taking 50 parts of glucose, 12 parts of potassium dihydrogen phosphate, wheat bran is 6 parts, 10 parts of ammonium chloride, 10 parts of potassium chloride, 8 parts of magnesium sulfate, chlorine Change 4 parts of sodium；Above-mentioned raw materials are stirred at normal temperature, 1kg/ bags is packaged into and spreads cultivation the culture medium of lactic acid bacteria to get pool mouth.
- The propagation method of lactic acid bacteria 5. a kind of pool mouth spreads cultivation, it is characterized in that: using following processing step:Step 1, preparation of culture medium: its formula rate is according to parts by weight: taking 40-60 parts of glucose, potassium dihydrogen phosphate 5-15 Part, wheat bran are 1-10 parts, 5-20 parts of ammonium chloride, 5-10 parts of potassium chloride, 5-10 parts of magnesium sulfate, 5-10 parts of sodium chloride；By above-mentioned original Material is stirred 30-80 minutes through trough type mixing machine at normal temperature, 20-70 revs/min of revolving speed, is packaged into 1kg/ bags to get the pool Mouth spreads cultivation the culture medium of lactic acid bacteria, spare；Step 2 removes chlorine residue: taking the empty barrel with cover of 50-100L, fills tap water, and gas explosion 24-36h removes chlorine residue；Step 3, mixed bacteria liquid preparation: take the pool mouth spread cultivation lactic acid bacteria culture medium 1-3kg be added to it is quick-fried cross gas water in, then plus Enter the lactic acid bacteria 1-3kg for needing to spread cultivation, is stirred 5-10 minutes with 10-20 revs/min of speed and obtain mixed bacteria liquid, cover bucket Lid；Fermentation: step 4 mixed bacteria liquid obtained in step 3 is placed in the sun, is issued in 15-25 DEG C of temperature condition It ferment 4-6 days, is rocked in fermentation process daily and obtains pool mouth for 1-3 times and spread cultivation lactic acid bacteria.
- The propagation method of lactic acid bacteria 6. a kind of pool mouth as claimed in claim 5 spreads cultivation, it is characterized in that: by obtained in step 3 Mixed bacteria liquid is placed in the cool, 26-35 DEG C of fermentation temperature, is fermented 2-4 days, is rocked 1-3 times daily in fermentation process and obtain the pool Mouth spreads cultivation lactic acid bacteria.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107338206A (en) * | 2017-07-24 | 2017-11-10 | 青岛金典生化器材有限公司 | A kind of culture medium for being enriched with Bacillus acidi lactici and preparation method thereof |
| CN107581122A (en) * | 2017-09-13 | 2018-01-16 | 寿县绿园特种水产生态养殖加工有限责任公司 | It is a kind of to carry out fresh water aquaculture method for penaeus vannamei using biological flocculation technology |
| CN109456914A (en) * | 2018-11-19 | 2019-03-12 | 江苏渔多多生物科技有限公司 | One kind low temperature resistant lactic acid bacteria used for aquiculture and its application |
-
2019
- 2019-05-08 CN CN201910380208.3A patent/CN109971690A/en not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107338206A (en) * | 2017-07-24 | 2017-11-10 | 青岛金典生化器材有限公司 | A kind of culture medium for being enriched with Bacillus acidi lactici and preparation method thereof |
| CN107581122A (en) * | 2017-09-13 | 2018-01-16 | 寿县绿园特种水产生态养殖加工有限责任公司 | It is a kind of to carry out fresh water aquaculture method for penaeus vannamei using biological flocculation technology |
| CN109456914A (en) * | 2018-11-19 | 2019-03-12 | 江苏渔多多生物科技有限公司 | One kind low temperature resistant lactic acid bacteria used for aquiculture and its application |
Non-Patent Citations (1)
| Title |
|---|
| 吴立峰等: "通威乳酸菌塘口简易扩培方法", 《HTTP://WWW.SHUICHAN.CC/ARTICLE_VIEW-37549.HTML》 * |
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