CN109966285B - Application of degreased cinnamon polyphenol extract in preparation of products for preventing and treating diabetes cognitive dysfunction - Google Patents

Application of degreased cinnamon polyphenol extract in preparation of products for preventing and treating diabetes cognitive dysfunction Download PDF

Info

Publication number
CN109966285B
CN109966285B CN201910227299.7A CN201910227299A CN109966285B CN 109966285 B CN109966285 B CN 109966285B CN 201910227299 A CN201910227299 A CN 201910227299A CN 109966285 B CN109966285 B CN 109966285B
Authority
CN
China
Prior art keywords
cinnamon
degreased
polyphenol
extract
cognitive dysfunction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201910227299.7A
Other languages
Chinese (zh)
Other versions
CN109966285A (en
Inventor
夏星
尹日凤
李宇清
梁明坤
刘思彤
钟静
霍丽妮
韦建华
丘芬
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guangxi University of Chinese Medicine
Original Assignee
Guangxi University of Chinese Medicine
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guangxi University of Chinese Medicine filed Critical Guangxi University of Chinese Medicine
Priority to CN201910227299.7A priority Critical patent/CN109966285B/en
Publication of CN109966285A publication Critical patent/CN109966285A/en
Application granted granted Critical
Publication of CN109966285B publication Critical patent/CN109966285B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
    • A61K31/35—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom
    • A61K31/352—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom condensed with carbocyclic rings, e.g. methantheline 
    • A61K31/353—3,4-Dihydrobenzopyrans, e.g. chroman, catechin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/54—Lauraceae (Laurel family), e.g. cinnamon or sassafras

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Diabetes (AREA)
  • Neurosurgery (AREA)
  • Organic Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biomedical Technology (AREA)
  • Neurology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Psychiatry (AREA)
  • Hospice & Palliative Care (AREA)
  • Emergency Medicine (AREA)
  • Endocrinology (AREA)
  • Hematology (AREA)
  • Obesity (AREA)
  • Epidemiology (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

The invention belongs to the technical field of medicinal natural products, and particularly relates to application of a degreased cinnamon polyphenol extract extracted from cinnamon bark in preparation of a product for preventing and treating diabetes cognitive dysfunction. The weight content of the total polyphenol in the degreased cinnamon polyphenol extract is 40% -80%, and the main effective component in the total polyphenol is one or a mixture of cinnamon tannin and cinnamon epicatechin; the effective components can improve learning and cognitive ability of diabetic cognitive disorder rats, increase antioxidant factors such as superoxide dismutase (SOD) and reduced Glutathione (GSH) in brain of diabetic cognitive disorder rats, relieve oxidation injury of nervous system, and reduce contents of Malondialdehyde (MDA), Nitrogen Oxide (NOX), and nitric oxide synthase (iNOS). The defatted cinnamon polyphenol extract provided by the invention has the effect of improving the cognitive dysfunction of diabetes, and is expected to be used for preventing and treating diseases such as the cognitive dysfunction of diabetes and the like.

Description

Application of degreased cinnamon polyphenol extract in preparation of products for preventing and treating diabetes cognitive dysfunction
Technical Field
The invention belongs to the technical field of medicinal natural products, and particularly relates to application of a degreased cinnamon polyphenol extract extracted from cinnamon bark in preparing a product for preventing and treating diabetes cognitive dysfunction, in particular to application of the degreased cinnamon polyphenol extract in preparing food and medicines related to improving the diabetes cognitive dysfunction nerve injury and preventing the diabetes cognitive dysfunction.
Background
As early as 2013, 1.1 hundred million diabetics in China are the first global diabetic patients, wherein the type II diabetes accounts for more than 90%. The cognitive impairment caused by diabetes is a common complication of type II diabetes, the incidence rate of the complication cognitive impairment of type II diabetes patients is 1.5 times that of non-diabetic patients, and 60-70% of the diabetic patients have cognitive impairment. Type ii diabetes is an independent risk factor for cognitive dysfunction and fails to significantly improve cognitive dysfunction in diabetic patients by effectively controlling blood glucose. The pathogenesis of the cognitive dysfunction of type II diabetes is very complex, and the clinical curative effect is not ideal as yet is not completely clarified. Therefore, the effective prevention and control of type II diabetes cognitive dysfunction is urgent, and the search for effective traditional Chinese medicine components for improving type II diabetes cognitive dysfunction has important clinical application value.
Cinnamon, [ Latin school name ]Cinnamomum cassia PreslThe dry bark is derived from the dry bark of the cinnamomum cassia presl, which is widely applied to food, medical treatment and other aspects in China, and is mainly produced in Guangxi. Cinnamon has been used as a traditional Chinese medicinal material for thousands of years. As early as in Shen nong Ben Cao Jing, there is a discussion on cinnamon which is used for a long time to relax the spirit and make the body light and not old. In the book Shang Zhong Jing of Shang Han za Bing Lun, 29 formulas derived from cinnamon/cinnamon twig as ancestor formula are included. Cortex Cinnamomi has effects of tonifying fire, supporting yang, guiding fire, expelling cold, relieving pain, promoting blood circulation, and dredging channels. Cinnamon mainly contains volatile oil, diterpene and glucoside thereof, flavanol and polymer thereof, flavonoid, polyphenol and other compounds, wherein cinnamon polyphenol is the main active component of the non-volatile part. At present, cinnamon has applications in the aspects of diabetes treatment, anti-inflammation, antimicrobial, anticancer, lipid reduction and cardiovascular resistance, and cinnamon has reports on the aspect of neuroprotection, for example, cinnamon has good prevention and treatment effects on Parkinson's disease, Alzheimer's disease, senile dementia and other neurological diseases [ Yangwua ] cinnamon is used for exploring shallow analysis on ischemic stroke excitotoxicity [ J]Eastern diet therapy and health, 2018, (1): 409.]. More interestingly, cinnamon and its metabolites can induce brain-derived cytokine expression, as well as neurotrophic factor expression in neuronal and glial cells, demonstrating the potential of cinnamon as a primary or auxiliary drug for the treatment of central nervous system disorders [ Zhengna, Xuhong, Luchang, et al]The journal of Chinese comparative medicine 2019, 29(1): 114-.]。
For the role of cinnamon in diabetes, the following documents were retrieved:
1. the protective effect of cinnamon polyphenol on streptozotocin-induced diabetic mice; [ authors ] Polygon villa, Xulingyuan, Wangjinni, Tanshan, Weilimei; an institution, second subsidiary hospital of Guangxi medical university; journal of zhong' an university of transportation bulletin (medical edition), 2019, 40 (01): 162-166. [ Abstract ] purpose: discussing the protective effect and molecular mechanism of cinnamon polyphenol on streptozotocin-induced diabetic mice. The method comprises the following steps: establishing a single-dose streptozotocin 240 mg/kg induced diabetes mouse model, and randomly dividing the model into 5 groups: the streptozotocin model control group, the metformin positive group (0.3 g/kg) and the cinnamon polyphenol low, medium and high dose groups (0.3, 0.6 and 1.2 g/kg) are continuously infused with stomach every day for 14 days. And a normal control group was set. After the last administration, the fasting blood glucose is measured by a blood glucose test paper, the serum amylopsin content is measured by a biochemical method, the serum insulin and glucagon concentrations are measured by an enzyme-linked immunosorbent assay method, the number of insulin positive cells in islet cells is detected by an immunohistochemical method, and the protein kinase B (AKT) and the phosphorylation level thereof in pancreatic cells are detected by a protein immunoblotting (Western blot) method. As a result: compared with mice in a streptozotocin model control group, the blood sugar of the mice in a cinnamon polyphenol intervention group is obviously reduced (P < 0.05), the serum insulin is increased, the glucagon is reduced (P < 0.05), the insulin positive cells in islet cells are obviously increased (P < 0.05), the protein kinase B (AKT) and the phosphorylation level thereof in pancreatic cells are simultaneously increased (P < 0.05), and the dosage dependence is shown. And (4) conclusion: cinnamon polyphenol has an effective cytoprotective effect on the pancreas damage of diabetic mice caused by streptozotocin, and the molecular mechanism of the cinnamon polyphenol can promote islet beta cells to secrete islets by regulating an islet cell AKT signal pathway, so that the pharmacological activity of reducing blood sugar is exerted.
Document 1 discloses that cinnamon can prevent and treat diabetes, and exerts the diabetes prevention and treatment effect by protecting pancreas damage and promoting insulin secretion. Although the same cinnamon extract as used in the present invention is cinnamon polyphenol, the document only reports the application of cinnamon polyphenol in diabetes, and does not report the cognitive dysfunction of diabetes.
2. The effect of cinnamon on hippocampal synaptic plasticity in diabetic rats; [ authors ] Yangtian; [ MEANS ] higher specialty schools in south Yang medicine; [ Canning name ] Chinese medicine Innovation 2015, 12(18) and 20-22. [ Abstract ] purpose: the influence of cinnamon on synaptotaxis plasticity of diabetic rats caused by streptozotocin is observed, and the possible mechanism of the synaptotaxis is discussed. The method comprises the following steps: the 27 rats were randomly divided into a control group, a diabetic group and a cinnamon group. A diabetes rat model is established by one-time injection of streptozotocin (65 mg/kg), a cinnamon group is intervened by gastric lavage cinnamon powder (5 g/kg) every day, electrophysiological experiments compare PS amplitude changes of a CA1 region of a hippocampus before and after tonic stimulation, and western blot experiments detect the expression of NR2B in brain tissues of the hippocampus. As a result: in electrophysiological experiments, the average PS amplification of a diabetic group after strong and direct stimulation is obviously lower than that of a control group, and cinnamon can improve the PS amplification of diabetic rats and increase the expression of NR2B in hippocampus. And (4) conclusion: cinnamon can improve the reduction of synaptic plasticity of diabetic rats, and the mechanism of cinnamon can up-regulate the expression of NR2B in hippocampus of diabetic rats.
The disclosure of the document 2 is similar to that of the present invention, but the document states that cinnamon can produce good effect on the hippocampus of diabetic rats, and does not relate to the application of cinnamon polyphenol in the aspect of diabetic cognitive dysfunction.
4. Application No. CN 201110202737.8; the invention name is as follows: a pharmaceutical composition for preventing and treating type 2 diabetes and its complications; the applicant: juxing Kanghua pharmaceutical science and technology, Inc., Tianjin; and (3) abstract: the invention belongs to the technical field of medicines, and discloses a pharmaceutical composition for treating diabetes and preventing and treating diabetic complications and a pharmaceutical application thereof. The pharmaceutical composition of the invention comprises a chemical drug and a traditional Chinese medicine extract, and specifically comprises metformin hydrochloride and a cinnamon extract. The pharmaceutical composition has good effect of treating diabetes, effectively controls the occurrence of diabetic complications, has low adverse reaction, is safer and more reliable than the single use of chemical drugs, and can reduce the dosage of metformin hydrochloride.
5. Application No.: CN 201210502442.7; the invention name is as follows: application of procyanidin in preparing medicine for treating diabetes; the applicant: wuhan Biotechnology research institute of Wuhan Baiao Jing biomedicine GmbH; and (3) abstract: the invention relates to application of procyanidine in a traditional Chinese medicine cinnamon water extract in preparation of a medicament for treating diabetes. The islet amyloid polypeptide is aggregated in vivo, and the aggregation process can destroy the structure of an islet beta cell membrane, induce beta cell apoptosis and damage the function of beta cells, and is considered to be one of important pathogenic causes of type 2 diabetes. The invention discovers that procyanidine in the traditional Chinese medicine cinnamon water extract can effectively inhibit the aggregation of islet amyloid polypeptide in type 2 diabetes, and simultaneously reverses the toxicity generated to beta cells in the aggregation process, thereby protecting the beta cells. The invention discovers that the cinnamon procyanidine can take the anti-islet amyloid polypeptide aggregation as a target spot, and has great application value in preparing the medicament for treating type 2 diabetes
6. Application No.: CN 02829018.6; the invention name is as follows: cinnamic acid dimers, their preparation and their use in the treatment of neurodegenerative diseases; the applicant: korea institute of science and technology, kyo corporation; and (3) abstract: the present invention relates to cinnamic acid dimers, their formulations and their use in the treatment of neurodegenerative diseases, which have excellent effects on enhancing learning and memory retention in vivo and have less side effects by not showing hormonal properties even when administered for a long period of time, and thus can be used for neurodegenerative diseases including dementia.
The contents disclosed in patent document 4, patent document 5, and patent document 6 are similar to the contents of the present invention. Although cinnamon is used for extraction, patent document 4, patent document 5, and patent document 6 use cinnamon extract, procyanidin, and cinnamic acid dimer, respectively, but are different from the present invention. Patent document 4 discloses that cinnamon is effective in controlling diabetic complications, but the ingredients of cinnamon extract used are not clear. Patent document 5 discloses that procyanidin is obtained by extracting cinnamon with water, but the extract obtained by the present invention is very different from patent document 5 because defatted cinnamon is used for extraction. Patent document 6 relates to application of cinnamon to neurological diseases, but cinnamic acid dimers are used as fat-soluble components of cinnamon, and diabetic cognitive dysfunction is not involved. Therefore, patent documents 4, 5, and 6 are greatly different from the present invention.
Disclosure of Invention
The invention aims to provide application of a degreased cinnamon polyphenol extract extracted from cinnamon in preparation of a product for preventing and treating diabetes cognitive dysfunction, and particularly relates to new application of the degreased cinnamon polyphenol extract in preparation of foods and medicines related to improvement of diabetic cognitive dysfunction nerve injury and prevention of diabetes cognitive dysfunction.
Furthermore, the main effective component of the defatted cinnamon polyphenol extract is one or a mixture of two of cinnamon tannin and cinnamon epicatechin, one or a mixture of two of cinnamon tannin and epicatechin extracted from cinnamon can improve the learning cognitive ability of rats with cognitive impairment of diabetes, increase antioxidant factors such as superoxide dismutase (SOD), reduced Glutathione (GSH) and the like in brains of rats with cognitive impairment of diabetes, and reduce the contents of Malondialdehyde (MDA), Nitric Oxide (NOX), nitric oxide synthase (iNOS) and the like. Cinnamon tannin and cinnamon epicatechin have the following structural formulae:
Figure 929300DEST_PATH_IMAGE001
the cinnamon tannin and the cinnamon epicatechin are mixed in any proportion.
The invention also provides an extraction method of the degreased cinnamon polyphenol extract, which is specifically divided into two steps, namely, the first step of supercritical degreasing to obtain degreased cinnamon and the second step of solvent extraction to obtain the cinnamon polyphenol extract.
In the extraction method of the defatted cinnamon polyphenol extract, the specific steps of the first step of supercritical degreasing to obtain defatted cinnamon are as follows: pulverizing cortex Cinnamomi, and adding supercritical CO2In an extraction kettle, the extraction pressure is 25-45 MPa, the extraction temperature is 30-75 ℃, the separation I pressure is 10-30 MPa, the separation I temperature is 25-75 ℃, the separation II pressure is 4-10 MPa, the separation II temperature is 25-55 ℃, and CO is added2Extracting the raw materials with the flow of 10-30 kg per kilogram of raw materials per hour for 2-10 hours, and taking out the degreased cinnamon from the extract. The second step of extracting cinnamon polyphenol extract by using solvent comprises the following specific steps: adding 45-75% by volume of ethanol according to the proportion of 1: 1-1: 9 times of the weight of the degreased cinnamon until the degreased cinnamon completely submerges a sample, extracting for 1-3 times, extracting for 1-10 hours each time, keeping the temperature at 30-80 ℃, merging filtrate, concentrating, adsorbing by using a DM130 macroporous resin column, sequentially washing by using pure water and 10-40% by mass of ethanol, eluting by using 40-80% by mass of ethanol, collecting eluent, and drying under reduced pressure to obtain an extract, wherein the total polyphenol content of the cinnamon is 40-80% by weight.
In the degreased cinnamon polyphenol extract extracted by the extracting method of the degreased cinnamon polyphenol extract, the weight content of total polyphenol is 40-80%; wherein the cinnamon tannin accounts for 30-55% of the total polyphenol weight content in the extract; the epicatechin accounts for 10-30% of the total polyphenol weight content in the extract.
The invention relates to application of a degreased cinnamon polyphenol extract extracted from cinnamon in preparation of a product for preventing and treating diabetes cognitive dysfunction, and the application of the degreased cinnamon polyphenol extract in improvement of the diabetes cognitive dysfunction and preparation of the product for assisting in improvement of the diabetes cognitive dysfunction.
The defatted cinnamon polyphenol improves cognitive dysfunction, and refers to the control of the defatted cinnamon polyphenol (comprising one or a mixture of two of cinnamon tannin and cinnamon epicatechin) for improving learning and memory ability, enhancing the activity of a brain tissue antioxidant system, reducing brain tissue oxidative stress injury, positioning aviation and space exploration latency, brain tissue SOD, GSH, MDA and related indexes of diabetes cognitive dysfunction diseases acknowledged by modern medicine.
The application of the defatted cinnamon polyphenol extract in preparing the product for improving cognitive dysfunction refers to the application of defatted cinnamon polyphenol (containing one or a mixture of two of cinnamon tannin and cinnamon epicatechin) in preparing food and medicines for improving the learning and memory ability of people with diabetes authentication dysfunction, relieving nerve injury and treating diabetes cognitive dysfunction.
Pharmacodynamic experiments of the inventor show that: the discovery of a rat model with diabetes cognitive dysfunction induced by high-fat feeding and small-dose streptozotocin of defatted cinnamon polyphenol shows that the study cognitive ability can be obviously improved, the activities of SOD and GSH of a brain tissue antioxidant system can be increased, and oxidation stress markers MDA, iNOS and NOX can be reduced after the administration of the defatted cinnamon polyphenol for 8 weeks, which indicates that the cinnamon polyphenol has a protective effect on the brain of the rat with diabetes cognitive dysfunction.
According to the above studies of the present inventors, the present invention provides a defatted cinnamon polyphenol extract having an effect of improving cognitive dysfunction in diabetes, which is expected to be used for the prevention and treatment of diseases such as cognitive dysfunction in diabetes.
The invention has the following advantages: the extraction method of the degreased cinnamon polyphenol extract provided by the invention is simple and feasible, and is suitable for industrial production; experiments show that the degreased cinnamon polyphenol improves the learning and memory ability, improves SOD and GSH, increases the oxidation resistance of brain tissues in a high-sugar environment, and has obvious effect of relieving oxidative stress damage of the brain caused by high sugar; the cinnamon oil extraction method has a good application prospect, cinnamon is rich in resources in China, material residues obtained after cinnamon oil is extracted industrially can be used as a raw material source of degreased cinnamon, and the raw material cost is low.
Detailed Description
To better illustrate the features and essence of the present invention, the following examples provide the extraction method of defatted cinnamon polyphenol extract and the results of pharmacological experiments of defatted cinnamon polyphenol to improve cognitive dysfunction of diabetes, illustrating its use in the pharmaceutical field.
Example 1
The application of the degreased cinnamon polyphenol extract in preparing the product for preventing and treating the cognitive dysfunction of the diabetes comprises one or a mixture of two of cinnamon tannin and cinnamon epicatechin, wherein the structural formulas of the cinnamon tannin and the cinnamon epicatechin are as follows:
Figure 687041DEST_PATH_IMAGE002
the cinnamon tannin and the cinnamon epicatechin are mixed in any proportion.
The extraction method of the degreased cinnamon polyphenol extract comprises two steps, wherein the two steps are respectively, firstly, supercritical degreasing is carried out to obtain degreased cinnamon, and secondly, the cinnamon polyphenol extract is extracted by a solvent. The first step of supercritical degreasing to obtain degreased cinnamon comprises the following specific steps: pulverizing cortex Cinnamomi, and adding supercritical CO2In an extraction kettle, the extraction pressure is 25-45 MPa, the extraction temperature is 30-75 ℃, the separation I pressure is 10-30 MPa, the separation I temperature is 25-75 ℃, the separation II pressure is 4-10 MPa, the separation II temperature is 25-55 ℃, and CO is added2Extracting the raw materials with the flow of 10-30 kg per kilogram of raw materials per hour for 2-10 hours, and taking out the degreased cinnamon from the extract. The second step of extracting cinnamon polyphenol extract by using solvent comprises the following specific steps: adding 45-75% by volume of ethanol according to the proportion of 1: 1-1: 9 times of the weight of the degreased cinnamon until the degreased cinnamon completely submerges a sample, extracting for 1-3 times, extracting for 1-10 hours each time, keeping the temperature at 30-80 ℃, merging filtrate, concentrating, adsorbing by using a DM130 macroporous resin column, sequentially washing by using pure water and 10-40% by mass of ethanol, eluting by using 40-80% by mass of ethanol, collecting eluent, and drying under reduced pressure to obtain the degreased cinnamon polyphenol extract; the weight content of the total polyphenol in the obtained degreased cinnamon polyphenol extract is 40-80%.
Example 2
A method for extracting defatted cortex Cinnamomi polyphenol extract comprisesTwo steps, namely, a first step of supercritical degreasing to obtain degreased cinnamon and a second step of solvent extraction of cinnamon polyphenol extract. The first step of supercritical degreasing to obtain degreased cinnamon comprises the following specific steps: pulverizing cortex Cinnamomi, and adding supercritical CO2In an extraction kettle, the extraction pressure is 25MPa, the extraction temperature is 30-75 ℃, the separation I pressure is 10MPa, the separation I temperature is 25-75 ℃, the separation II pressure is 4MPa, the separation II temperature is 25-55 ℃, and CO is in the extraction kettle2The flow rate was 10kg per hour per kg of starting material, the extraction was carried out for 10h, and the delipidated cinnamon was removed from the extract. The second step of extracting cinnamon polyphenol extract by using solvent comprises the following specific steps: adding 75% ethanol by volume according to the proportion of 1-9 times of the weight of the degreased cinnamon until the sample is completely submerged, extracting for 1 time, extracting for 10 hours each time, keeping the temperature at 30-80 ℃, combining the filtrate, concentrating, adsorbing by using a DM130 macroporous resin column, sequentially washing by using pure water and 10% ethanol by mass concentration, eluting by using 40% ethanol by mass concentration, collecting the eluent, and drying under reduced pressure to obtain the degreased cinnamon polyphenol extract.
Example 3
A method for extracting defatted cortex Cinnamomi polyphenol extract comprises two steps, respectively, first step supercritical defatting to obtain defatted cortex Cinnamomi, and second step solvent extracting cortex Cinnamomi polyphenol extract. The first step of supercritical degreasing to obtain degreased cinnamon comprises the following specific steps: pulverizing cortex Cinnamomi, and adding supercritical CO2In an extraction kettle, the extraction pressure is 35MPa, the extraction temperature is 30-75 ℃, the separation I pressure is 20MPa, the separation I temperature is 25-75 ℃, the separation II pressure is 6MPa, the separation II temperature is 25-55 ℃, and CO is in the extraction kettle2The flow rate was 20kg per hour per kg of starting material, the extraction was carried out for 8h, and the delipidated cinnamon was removed from the extract. The second step of extracting cinnamon polyphenol extract by using solvent comprises the following specific steps: adding 60% ethanol by volume according to the proportion of 6 times of the weight of the degreased cinnamon, extracting for 2 times, extracting for 7h each time, keeping the temperature at 30-80 ℃, merging filtrate, concentrating, adsorbing by using a DM130 macroporous resin column, sequentially washing by using pure water and 30% ethanol by mass, eluting by using 60% ethanol by mass, collecting eluent, and drying under reduced pressure to obtain the degreased cinnamon polyphenol extract.
Example 4
A method for extracting defatted cortex Cinnamomi polyphenol extract comprises two steps, respectively, first step supercritical defatting to obtain defatted cortex Cinnamomi, and second step solvent extracting cortex Cinnamomi polyphenol extract. The first step of supercritical degreasing to obtain degreased cinnamon comprises the following specific steps: pulverizing cortex Cinnamomi, and adding supercritical CO2In an extraction kettle, the extraction pressure is 45MPa, the extraction temperature is 30-75 ℃, the separation I pressure is 30MPa, the separation I temperature is 25-75 ℃, the separation II pressure is 10MPa, the separation II temperature is 25-55 ℃, and CO is in the extraction kettle2The flow rate was 30kg per hour per kg of starting material, the extraction was carried out for 2h, and the delipidated cinnamon was removed from the extract. The second step of extracting cinnamon polyphenol extract by using solvent comprises the following specific steps: adding 45% ethanol by volume according to a proportion of 9 times the weight of the degreased cinnamon, extracting for 3 times, extracting for 1 h each time, keeping the temperature at 30-80 ℃, merging filtrate, concentrating, adsorbing by using a DM130 macroporous resin column, sequentially washing by using pure water and 40% ethanol by mass, eluting by using 80% ethanol by mass, collecting eluent, and drying under reduced pressure to obtain the degreased cinnamon polyphenol extract.
Example 5
Cognitive function protection effect of defatted cinnamon polyphenol on diabetic cognitive dysfunction rats
1.1 reagent: defatted cinnamon polyphenol: the defatted cinnamon polyphenol extract obtained by extracting cinnamon as a raw material according to the extraction method of the defatted cinnamon polyphenol extract in example 3. Streptozotocin (STZ), a product of SIGMA corporation; donepezil tablets, Jiangsu Haofen pharmaceutical group, Inc.; blood glucose test paper, manufactured by roche diagnostic products (shanghai) limited; and (3) high-fat feed formula: 74.5 percent of basal feed, 1 percent of cholesterol, 5 percent of yolk powder, 10 percent of cane sugar, 9 percent of lard and 0.5 percent of sodium cholate; a superoxide dismutase (SOD) testing kit, a trace Malondialdehyde (MDA) testing kit, a trace reduced glutathione testing kit, an NADH oxidase kit, a Nitric Oxide Synthase (NOS) testing kit, and Nanjing is produced by a bioengineering research institute.
1.2 Experimental animals: SD rats, all male, weighing 180-.
1.3 Experimental methods: taking a plurality of SD male rats, feeding high-fat feed, arranging a blank group, and feeding common feed. After 6 weeks, all rats out of the blank group are injected with 35 mg/kg streptozotocin in the abdominal cavity, the blank group is injected with an equal amount of citrate buffer solution, blood sugar is measured for 1 time 72 hours after the injection of the streptozotocin, and the molding is considered to be successful if the blood sugar is more than 11.1 mmol/L. The rats successfully molded are randomly and uniformly divided into 5 groups according to blood sugar values: model group, high (400 mg/kg), medium (200 mg/kg), low (100 mg/kg) dose group of defatted cinnamon polyphenol, donepezil group (0.07 mg/kg). Administration group ig is administrated for 1 time/day, the blank group and the model group are filled with pure water with the same amount, the stomach is continuously drenched for 8 weeks, the blank group is fed with common feed during the experiment period, and the model group and each drug treatment group are continuously fed with high fat feed.
During the administration, fasting blood glucose levels were measured at 2, 4, 6, and 8 weeks after administration. The Morris water maze experiment was performed at week 8 of dosing to investigate the learning cognitive ability of the animals. After 8 weeks of administration, 7% chloral hydrate was anesthetized, and after exsanguination, brain tissue was decapitated and harvested for SOD, MDA, GSH, iNOS, and NOX.
1.4 statistical analysis: the results are all expressed as mean ± standard deviation. Differences between groups were analyzed using the t-test.
1.5 Experimental results: the fasting blood glucose levels in the model group were maintained at higher levels throughout the treatment period. 4 weeks after treatment, a significant hypoglycemic effect (P < 0.05) was observed at the high dose of defatted cinnamon polyphenol compared to the model group and continued until week 8 (P <0.05 compared to the model group); after 6 weeks of administration, significant hypoglycemic effects (P < 0.05) were observed in both the medium and low doses of defatted cinnamon polyphenol, and continued for 8 weeks after administration. The result shows that the degreased cinnamon polyphenol has a stable blood sugar reducing effect on diabetic nephropathy rats. The data are shown in table one.
Figure 23606DEST_PATH_IMAGE003
In the Morris water maze test of rats with cognitive dysfunction caused by diabetes, the study ability levels of donepezil and degreased cinnamon polyphenol are obviously improved in 400mg/kg, 200mg/kg and 100mg/kg compared with the model group (P <0.01 or P < 0.05). In the 5-day experiment of Morris water maze learning, the escape latency of rats in the donepezil and degreased cinnamon polyphenol administration groups is shortened along with the increase of the training days in training, and the escape latency of the donepezil and degreased cinnamon polyphenol groups of 400, 200 and 100mg/kg is remarkably shortened by the fifth day (compared with the model group, P is less than 0.01). The defatted cinnamon polyphenol has the function of improving the learning and memory capacity of diabetic cognitive dysfunction rats. The data are shown in table two.
Figure 501992DEST_PATH_IMAGE004
The activity percentage of animals in the defatted cinnamon polyphenol 200, 100mg/kg group on the platform quadrant was already significantly higher than that in the model group (P < 0.01) on the third day of the Morris water maze experiment, and the activity percentage of animals in the donepezil group and the defatted cinnamon polyphenol 400, 200, 100mg/kg group on the fourth and fifth days was significantly higher than that in the model group (P < 0.01). The defatted cinnamon polyphenol is proved to be beneficial to the improvement of the learning and memory capacity of the diabetic cognitive dysfunction rat. The data are shown in table three.
Figure 738939DEST_PATH_IMAGE005
And removing the platform from the last day of the Morris water maze experiment to carry out a space exploration experiment. Compared with the model group, the donepezil group and the degreased cinnamon polyphenol group have the obviously increased times of crossing the platform (P < 0.01) in 400mg/kg, 200mg/kg and 100 mg/kg. The application shows that the treatment of the defatted cinnamon polyphenol can effectively improve the cognitive dysfunction of diabetic rats, and has the effects of enhancing the space exploration capacity and relieving the decline of the cognitive function. See table four.
Figure 402001DEST_PATH_IMAGE006
In order to further verify the protection effect of the defatted cinnamon polyphenol on brain tissues, the brain tissue oxidative stress related indexes of various groups of animals are detected. Compared with the model group, the rat brain tissue SOD activity and GSH content of the degreased cinnamon polyphenol 100mg/kg group and the rat brain tissue SOD activity and GSH content of the degreased cinnamon polyphenol 400mg/kg group are obviously improved (P <0.05 or P < 0.01), and simultaneously the MDA content is obviously reduced (P < 0.01). In addition, the SOD activity and the GSH content of brain tissues of a 200mg/kg group of degreased cinnamon polyphenol also show a remarkable increasing trend. The application shows that after the degreased cinnamon polyphenol is treated, the antioxidant capacity of brain tissues of diabetic rats can be effectively improved, and the body is protected from nerve damage caused by oxidative stress. The data are shown in table five.
Figure 599764DEST_PATH_IMAGE007
In the brain tissues of rats with cognitive dysfunction caused by diabetes, iNOS activity and NOX content of degreased cinnamon polyphenol 400, 200 and 100mg/kg groups are obviously reduced (P is less than 0.01). The application of the defatted cinnamon polyphenol to the diabetic cognitive dysfunction rat can effectively reduce the content of nitric oxide and reduce the damage of the nitric oxide to brain tissues. The data are shown in Table six.
Figure 678579DEST_PATH_IMAGE008
1.6 conclusion: experiments of the inventor show that the defatted cinnamon polyphenol can obviously relieve the fasting blood glucose rise of rats with diabetic cognitive dysfunction, protect the space exploration capacity and learning and memory capacity of the rats with diabetes, obviously increase the contents of SOD and GSH antioxidants, and reduce the levels of MDA, iNOS, NOX and other stress markers, so that the defatted cinnamon polyphenol can relieve the cognitive dysfunction and nerve injury of the rats with diabetic cognitive dysfunction and improve the diabetic cognitive dysfunction.

Claims (3)

1. The method for extracting the defatted cinnamon polyphenol extract comprises two steps, namely, the first step of supercritical degreasing to obtain defatted cinnamon and the second step of solvent extraction of the cinnamon polyphenol extract;
the first step of supercritical degreasing to obtain degreased cinnamon comprises the following specific steps: pulverizing cortex Cinnamomi, and adding supercritical CO2In an extraction kettle, the extraction pressure is 25-45 MPa, the extraction temperature is 30-75 ℃, the separation I pressure is 10-30 MPa, the separation I temperature is 25-75 ℃, the separation II pressure is 4-10 MPa, the separation II temperature is 25-55 ℃, and CO is added2Extracting the raw materials with the flow of 10-30 kg per kilogram of raw materials per hour for 2-10 hours, and taking out the degreased cinnamon from the extract;
the second step of extracting cinnamon polyphenol extract by using solvent comprises the following specific steps: adding 45-75% by volume of ethanol according to the proportion of 1: 1-1: 9 times of the weight of the degreased cinnamon until the degreased cinnamon completely submerges a sample, extracting for 1-3 times, extracting for 1-10 hours each time, keeping the temperature at 30-80 ℃, merging filtrate, concentrating, adsorbing by using a DM130 macroporous resin column, sequentially washing by using pure water and 10-40% by mass of ethanol, eluting by using 40-80% by mass of ethanol, collecting eluent, and drying under reduced pressure to obtain an extract, wherein the total polyphenol content of the cinnamon is 40-80% by weight.
2. The use of the defatted cinnamon polyphenol extract of claim 1 in the preparation of a product for the prevention and treatment of diabetic cognitive dysfunction, wherein: the degreased cinnamon polyphenol extract is used for preparing medicines for improving diabetic cognitive dysfunction nerve injury and preventing diabetic cognitive dysfunction.
3. The use of the defatted cinnamon polyphenol extract of claim 1 in the preparation of a product for the prevention and treatment of diabetic cognitive dysfunction, wherein: in the degreased cinnamon polyphenol extract extracted by the extracting method of the degreased cinnamon polyphenol extract, the weight content of total polyphenol is 40% -80%; wherein the cinnamon tannin accounts for 30-55% of the total polyphenol weight content in the extract; the cinnamon epicatechin accounts for 10% -30% of the total polyphenol weight content in the extract; the structural formulas of the cinnamon tannin and the cinnamon epicatechin are as follows:
Figure 711189DEST_PATH_IMAGE001
。
CN201910227299.7A 2019-03-25 2019-03-25 Application of degreased cinnamon polyphenol extract in preparation of products for preventing and treating diabetes cognitive dysfunction Active CN109966285B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201910227299.7A CN109966285B (en) 2019-03-25 2019-03-25 Application of degreased cinnamon polyphenol extract in preparation of products for preventing and treating diabetes cognitive dysfunction

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201910227299.7A CN109966285B (en) 2019-03-25 2019-03-25 Application of degreased cinnamon polyphenol extract in preparation of products for preventing and treating diabetes cognitive dysfunction

Publications (2)

Publication Number Publication Date
CN109966285A CN109966285A (en) 2019-07-05
CN109966285B true CN109966285B (en) 2021-09-21

Family

ID=67080295

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201910227299.7A Active CN109966285B (en) 2019-03-25 2019-03-25 Application of degreased cinnamon polyphenol extract in preparation of products for preventing and treating diabetes cognitive dysfunction

Country Status (1)

Country Link
CN (1) CN109966285B (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112891394A (en) * 2021-03-02 2021-06-04 广西壮族自治区中医药研究院 Application of cinnamon extract in preparation of hypoglycemic drugs

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8895083B2 (en) * 2008-10-28 2014-11-25 Allegiance Equity Corporation Composition for the maintenance of blood sugar levels comprising cinnamon and ginseng

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8895083B2 (en) * 2008-10-28 2014-11-25 Allegiance Equity Corporation Composition for the maintenance of blood sugar levels comprising cinnamon and ginseng

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
The effect of Cinnamomum zeylanicum bark water extract on memory performance in alloxan-induced diabetic mice;Azadeh Mesripour等;《Research in Pharmaceutical Sciences》;20160731;第11卷(第4期);第318-323页 *
脱脂肉桂粉提取肉桂多酚的研究;李英奇等;《中草药》;20171130;第48卷(第21期);第4448-4452页 *

Also Published As

Publication number Publication date
CN109966285A (en) 2019-07-05

Similar Documents

Publication Publication Date Title
Liu et al. Nitric oxide synthase in hypoxic or ischemic brain injury
Yin et al. Berberine improves glucose metabolism through induction of glycolysis
Yamamoto et al. Amla enhances mitochondrial spare respiratory capacity by increasing mitochondrial biogenesis and antioxidant systems in a murine skeletal muscle cell line
CN104491350B (en) A kind of health products for adjusting cardiovascular and cerebrovascular health and reducing cardiovascular and cerebrovascular disease risk
EP3199171B1 (en) Pharmaceutical composition for treating and preventing degenerative neurological disorders, containing, as active ingredient, mixture extract of moutan root bark, angelica dahurica root and bupleurum root or fraction thereof
Kumar et al. Evaluation of antidiabetic activity of Gymnema sylvestre and Andrographis paniculata in streptozotocin induced diabetic rats
JP6045845B2 (en) GLP-1 secretion promoter
CN109966285B (en) Application of degreased cinnamon polyphenol extract in preparation of products for preventing and treating diabetes cognitive dysfunction
Choi et al. Anti‐aging effects of Schisandrae chinensis Fructus extract: improvement of insulin sensitivity and muscle function in aged mice
KR20140120667A (en) A composition comprising the extract of Salix genus plant for treating and preventing neuro-degenerative disease
CN106822166B (en) A kind of drug for preventing and treating diabetes and hyperlipidemia and its application in pharmacy
KR20180130268A (en) Composition for preventing, alleviating and treating neurodegenerative diseases comprising Sargassum honeri extracts
CN106822338B (en) Compound composition for reducing blood sugar and blood fat and preventing and/or treating diabetes and complications thereof and application thereof
KR100979459B1 (en) Tetracera scandens extracts and 4H-chromen-4-one derivatives isolated therefrom increasing glucose uptake in differentiated L6 muscle cells
CN109966283B (en) Application of degreased cinnamon polyphenol extract in preparation of product for preventing and treating diabetic nephropathy
US20080311228A1 (en) Pharmaceutical composition for protecting neurons comprising extract of lithospermum erythrothizon sieb. et. zucc or acetylshikonin isolated therefrom as an effective ingredient
Umar et al. In vivo antihyperglycaemic activity of crude and partitioned fractions of selected medicinal plants
Deng et al. Panax ginseng abuse exhibits a pro‐inflammatory effect by activating the NF‐κB pathway
CN109806287B (en) General flavone glycoside of folium Microcoris paniculatae, and preparation method and application thereof
CN106822095A (en) A kind of medicine and its application in pharmacy for preventing and treating fatty liver and obesity
Tian et al. Daidzin decreases blood glucose and lipid in streptozotocin-induced diabetic mice
CN103006634A (en) Use of hypericum sampsonii hance xanthone compounds in antidepressant drug preparation
KR101907179B1 (en) Method for production of sulforaphene-enriched raphanus sativus seeds extracts and Food composition, pharmaceutical composition, animal medicines for weight and blood glucose control, fatty liver prevention with the raphanus sativus seeds extracts therefrom
CN101407531B (en) Use of dimer hydrolysable tannins derivative in preparing medicament and diet additive for treating obesity
RU2582282C1 (en) Method of producing agent having stress protective and anti-hypoxic activity

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant