CN109966285A - Application of the degreasing cinnamon polyphenol extract in preparation prevention and treatment diabetes cognition dysfunction product - Google Patents
Application of the degreasing cinnamon polyphenol extract in preparation prevention and treatment diabetes cognition dysfunction product Download PDFInfo
- Publication number
- CN109966285A CN109966285A CN201910227299.7A CN201910227299A CN109966285A CN 109966285 A CN109966285 A CN 109966285A CN 201910227299 A CN201910227299 A CN 201910227299A CN 109966285 A CN109966285 A CN 109966285A
- Authority
- CN
- China
- Prior art keywords
- degreasing
- cortex cinnamomi
- polyphenol extract
- cinnamon
- extract
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
- A61K31/35—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom
- A61K31/352—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom condensed with carbocyclic rings, e.g. methantheline
- A61K31/353—3,4-Dihydrobenzopyrans, e.g. chroman, catechin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
- A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/54—Lauraceae (Laurel family), e.g. cinnamon or sassafras
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Diabetes (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Engineering & Computer Science (AREA)
- Veterinary Medicine (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicinal Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Neurosurgery (AREA)
- Neurology (AREA)
- Biomedical Technology (AREA)
- Psychiatry (AREA)
- Hospice & Palliative Care (AREA)
- Emergency Medicine (AREA)
- Endocrinology (AREA)
- Hematology (AREA)
- Obesity (AREA)
- Epidemiology (AREA)
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention belongs to medicinal natural products technical fields, and in particular to a kind of application from the degreasing cinnamon polyphenol extract extracted in cassia in preparation prevention and treatment diabetes cognition dysfunction product.Total polyphenols weight content is 40% ~ 80% in the degreasing cinnamon polyphenol extract, and the principle active component in total polyphenols is the mixture of cortex cinnamomi tannin and cortex cinnamomi epicatechin either or both of them；The effective component can be improved diabetes cognitive disorder rat learning cognition ability, increase the Antioxidative Factors such as diabetes cognition dysfunction SOD in Brain of Rats (SOD), reduced glutathione (GSH), mitigate nervous system oxidative damage, reduces malonaldehyde (MDA), nitrogen oxides (NOX), nitricoxide synthase (iNOS) equal size.Degreasing cinnamon polyphenol extract provided by the invention has the function of improving diabetes cognition dysfunction, is expected to be used for preventing and treating the diseases such as diabetes cognition dysfunction.
Description
Technical field
The invention belongs to medicinal natural products technical field more particularly to a kind of degreasing cortex cinnamomi extracted from cassia are more
Application of the phenol extraction object in preparation prevention and treatment diabetes cognition dysfunction product, specifically degreasing cinnamon polyphenol extract are being made
In terms of standby improvement diabetes cognition dysfunction neurotrosis and prevention diabetes cognition dysfunction relevant food and drug
Purposes.
Background technique
Early in 2013, the diabetic in China was ranked the first in the world with 1.1 hundred million diabetics, wherein II type
Diabetes account for 90% or more.Diabetes cognitive disorder is the common complication of type II diabetes, and patients with NIDDM is concurrently recognized
The disease incidence for knowing dysfunction is 1.5 times of non-diabetic patients, and having 60% ~ 70% in diabetic, there are cognitive function barriers
Hinder.Type II diabetes is the independent hazard factor of cognition dysfunction, fails to be obviously improved diabetes by effectively controlling blood glucose
The cognition dysfunction of patient.The pathogenesis of type II diabetes cognition dysfunction is extremely complex, not yet illustrates completely so far,
Its clinical efficacy is unsatisfactory.Therefore, effectively prevention and control type II diabetes cognition dysfunction seem especially urgent, find
There is important clinical value with the effective component of chinese medicine for improving type II diabetes cognition dysfunction.
Cortex cinnamomi, [Classification system]Cinnamomum cassia Presl, from the dry bark of lauraceae plant cortex cinnamomi,
China be widely used in food, in terms of, main product is in Guangxi.Cortex cinnamomi is as the application in existing thousand of traditional Chinese medicine
History.Early in just there is the discussion " osmanthus, the logical mind of long term usage are made light of one's life by commiting suicide not old " to cortex cinnamomi in Shennong's Herbal.In " the typhoid fever of Zhang Zhongjing
Miscellaneous diseases opinion " in cortex cinnamomi/ramulus cinnamomi be that Zu Fang and derivative prescription reach 29 head.Cortex cinnamomi has mends fire supporing yang, let the fire back to its origin, cold dispelling
Analgesic, the effect of blood circulation.Volatile oil, diterpene and its glucosides, flavanols and its polymer, flavones are mainly contained in cortex cinnamomi
The multiple compounds such as class, Polyphenols, wherein cortex cinnamomi polyphenol is then the main active of non-volatile fraction.Currently, cortex cinnamomi in addition to
Have outside in terms for the treatment of diabetes, anti-inflammatory, antimicrobial, anticancer, lipid-loweringing and anti-angiocarpy, cortex cinnamomi makees in neuroprotection
It is also had been reported that with aspect, for example has good prevention and treatment in neurogenic diseases such as Parkinson's disease, Alzheimer's disease, senile dementias
Effect [exploration simple analysis [J] the east dietotherapy and health care that Yang Wuhua cortex cinnamomi acts on ishemic stroke excititoxic,
2018, (1): 409.].It is furthermore interesting that cortex cinnamomi and its metabolite can induce brain source nerve factor expression, and nerve
Expression of the trophic factors in neuronal cell and spongiocyte, it was demonstrated that cortex cinnamomi has as main or ancillary drug for treating
A possibility that central nervous system disease [Zheng Na, Xu Hong, Lu Changjun, wait cortex cinnamomi treatment the nervous system disease research into
Open up [J] China comparative medicine magazine, 2019,29 (1): 114-118.].
For effect of the cortex cinnamomi in terms of diabetes, following documents is retrieved:
1, [autograph] cortex cinnamomi polyphenol causes the protective effect of diabetic mice to streptozotocin；[author] Liao Zuozhuan, Xu Lingyuan, king
Jin Ni, Tan Hongdi, Wei Limei；The second affiliated hospital, [mechanism] Guangxi Medical University；[periodical name] Zhong An university of communications journal (medicine
Version), 2019,40 (01): 162-166.[digest] purpose: the protection that cortex cinnamomi polyphenol causes diabetic mice to streptozotocin is inquired into
Effect and its molecular mechanism.Method: 240 mg/kg induced diabetes mouse model of single dose streptozotocin is established, at random
It is divided into 5 groups: streptozotocin model control group, melbine positive group (0.3 g/kg) and cortex cinnamomi polyphenol low, middle and high dose groups
(0.3,0.6,1.2 g/kg), continues daily stomach-filling, totally 14 d.And Normal group is set.After the last administration, blood sugar test paper is surveyed
Determine fasting blood-glucose, biochemical method measures serum amylopsin content, and enzyme-linked immunosorbent assay measures serum insulin and pancreas is high
Blood glucose element concentration, Immunohistochemical Method detect insulin positive cells quantity in islet cells, protein immunoblot (Western
Blot) protein kinase B (AKT) and its phosphorylation level in method detection Stem Cells.As a result: with streptozotocin model control group
Mouse is compared, and the blood glucose of cortex cinnamomi polyphenol intervention group mouse is substantially reduced (P < 0.05), and serum insulin increases and glucagon
It reduces (P < 0.05), insulin positive cells dramatically increase (P < 0.05) in islet cells, protein kinase B in Stem Cells
(AKT) and its phosphorylation level increases (P < 0.05) simultaneously, and shows as certain dose dependent.Conclusion: cortex cinnamomi polyphenol pair
The damage of diabetic mice pancreas caused by streptozotocin shows effective cytoprotection, and molecular mechanism may pass through tune
It saves islet cells AKT signal path and promotes islet β cell pancreas islet, to play hypoglycemic pharmacological activity.
Document 1, which discloses cortex cinnamomi, can prevent and treat diabetes, and text promotes insulin secretion to play glycosuria by protection injury of pancreas
Sick preventive and therapeutic effect.It is cortex cinnamomi polyphenol although identical as cinnamomum cassia extract used in the present invention, the document only reports cortex cinnamomi
Application of the polyphenol in terms of diabetes, and have no the report in terms of diabetes cognition dysfunction.
2, influence of [autograph] cortex cinnamomi to Hippocampus of Diabetic Rats synaptic plasticity；[author] Yang Tan；[mechanism] Nanyang doctor
Learn higher junior college；[periodical name] Chinese medicine innovation, 2015,12 (18): 20-22.[digest] purpose: observation cortex cinnamomi is to chain urea
The influence of diabetes rat synaptic plasticity caused by rhzomorph, inquires into its mechanism.Method: 27 rats are randomly divided into control
Group, diabetes group and cortex cinnamomi group.Diabetes rat model by streptozotocin (65 mg/kg), disposably establish by injection, cortex cinnamomi group
Intervened daily by stomach-filling ground cinnamon (5 g/kg), Hippocampal CA 1 PS amplitude before and after tetanic stimulation is compared in electro physiology experiment
Variation, westernblot experiment detect the expression of NR2B in hippocampus brain tissue.As a result: in electro physiology experiment, diabetes group is tetanic
Average PS amplification is significantly lower than control group after stimulation, and cortex cinnamomi can improve diabetes rat PS amplification, and increase NR2B in hippocampus
Expression.Conclusion: cortex cinnamomi can improve the decline of diabetes rat synaptic plasticity, and mechanism and cortex cinnamomi can raise diabetes rat sea
The expression of horse NR2B is related.
2 disclosure of document and the present invention have a similarity, but what the document said is that cortex cinnamomi can be to diabetes rat
Hippocampus generates good function and effect, is not directed to application of the cortex cinnamomi polyphenol in terms of diabetes cognition dysfunction.
4, application number CN201110202737.8；Denomination of invention: one kind is for diabetes B and Complication prevention and treatment
Pharmaceutical composition；Applicant: Tianjin Juxing Kanghua Medical Technology Co., Ltd.；Abstract: the invention belongs to medical science necks
Domain discloses a kind of pharmaceutical composition and its pharmacy application for treating diabetes and preventing and treating diabetic complication.This hair
Bright pharmaceutical composition is made of a kind of chemicals and a kind of Chinese medical extract, specifically, by Metformin hydrochloride and meat
Osmanthus extract composition.Medicine composite for curing diabetes of the invention work well, and effectively control the hair of diabetic complication
Raw, adverse reaction is low, than using chemicals more secure and reliable merely, and can reduce the dosage of Metformin hydrochloride.
5, application number: CN201210502442.7；Denomination of invention: procyanidine answering in preparation treatment diabetes medicament
With；Applicant: hundred Wuhan Bioteknologisk Institut, Ao Jing biological medicine Co., Ltd of Wuhan；Abstract: the present invention relates to Chinese medicine meat
Application of the procyanidine in preparation treatment diabetes medicament in the water extract of osmanthus.Amylin occurs poly- in vivo
Collection, this accumulation process can destroy the structure of beta Cell of islet film, and inducing beta cell apoptosis damages the function of β cell, it is considered to be
One of important pathogenesis of diabetes B.Present invention discover that the procyanidine in Chinese traditional medicine cinnamon water extract can effectively inhibit 2
The aggregation of amylin in patients with type Ⅰ DM, while the toxicity generated in its accumulation process to β cell is reversed, to protect
β cell.Present invention discover that procyanidine in cassia can be using anti-islet amyloid polypeptide aggregation as target spot, in preparation treatment 2 types sugar
Urinating has great application value in medicine
6, application number: CN02829018.6；Denomination of invention: cortex cinnamomi acid dimer, their preparation and its treatment neurodegeneration
Application in disease；Applicant: Ke Ji Co., Ltd., Korean Institute of Science and Technology；Abstract: the present invention relates to cinnamic acid dimerization
Body, their preparation and its application in treatment neurodegenerative disease, in vivo to enhancing learning and memory holding capacity tool
There is an excellent effect, and even if when applying long-term, there is smaller side effect and not showing hormonal properties, therefore can
For including dull-witted neurodegenerative disease.
Patent document 4, patent document 5 and content disclosed Patent Document 6 and the contents of the present invention have similitude.Though
So to be extracted using cortex cinnamomi, patent document 4, patent document 5 and patent document 6 use respectively cinnamomum cassia extract,
Procyanidine, cortex cinnamomi acid dimer, but have larger difference with the present invention.Patent document 4, although disclosing cortex cinnamomi can be effectively controlled
The Fang Sheng of diabetic complication, but the cinnamomum cassia extract ingredient used is indefinite.Patent document 5, the procyanidine used are meat
Osmanthus carries out gained after water mentions, but the present invention is extracted using degreasing cortex cinnamomi, so extract obtained and patent document 5 of the invention
Very different.Although patent document 6 is related to application of the cortex cinnamomi in terms of neurogenic disease, cortex cinnamomi acid dimer is used,
For the liposoluble constituent of cortex cinnamomi, and it is not directed to diabetes cognition dysfunction.So patent document 4, patent document 5 and
There are larger differences with the present invention for patent document 6.
Summary of the invention
The object of the present invention is to provide the degreasing cinnamon polyphenol extracts extracted in a kind of cortex cinnamomi to prevent and treat diabetes in preparation
Application in cognition dysfunction product, specifically degreasing cinnamon polyphenol extract improve diabetes cognition dysfunction in preparation
New application in terms of neurotrosis and prevention diabetes cognition dysfunction relevant food and drug.
Further, the principle active component of heretofore described degreasing cinnamon polyphenol extract is cortex cinnamomi tannin and meat
One of which or two kinds of mixture in the epicatechin of osmanthus, the cortex cinnamomi tannin and epicatechin extracted from cortex cinnamomi its
One of or both mixture, can be improved diabetes cognitive disorder rat learning cognition ability, increase diabetes and recognize function
The Antioxidative Factors such as energy obstacle SOD in Brain of Rats (SOD), reduced glutathione (GSH) reduce malonaldehyde
(MDA), nitrogen oxides (NOX), nitricoxide synthase (iNOS) equal size.Cortex cinnamomi tannin and cortex cinnamomi epicatechin structural formula are such as
Under:
It is mixed in the mixture of the cortex cinnamomi tannin and both cortex cinnamomi epicatechins with arbitrary proportion.
Application of the above-mentioned degreasing cinnamon polyphenol extract in preparation prevention and treatment diabetes cognition dysfunction product, this hair
It is bright to additionally provide the extracting method of the degreasing cinnamon polyphenol extract, two steps are specifically divided into, respectively, the first step is overcritical de-
Rouge obtains degreasing cortex cinnamomi, second step solvent extraction cinnamon polyphenol extract.
In the extracting method of the degreasing cinnamon polyphenol extract, the overcritical degreasing of the first step obtains the tool of degreasing cortex cinnamomi
Steps are as follows for body: taking cassia to crush, is packed into supercritical CO2In extraction kettle, 25 ~ 45MPa of extracting pressure, 30 DEG C of extraction temperature ~
75 DEG C, 10 ~ 30MPa of I pressure is separated, separates 25 DEG C ~ 75 DEG C of I temperature, separates 4 ~ 10MPa of II pressure, separates II temperature 25 DEG C ~ 55
DEG C, CO2Per kilogram raw material, 2 ~ 10h of extraction take out degreasing cortex cinnamomi to 10 ~ 30kg of flow from extract per hour.Second step solvent
Extracting cinnamon polyphenol extract, specific step is as follows: volume fraction, which is added, in 1:1 ~ 1:9 times of weight of degreasing cortex cinnamomi of ratio is
45 ~ 75% ethyl alcohol extracts 1 ~ 3 time until flooding sample completely, extracts 1 ~ 10 h every time, and temperature is maintained at 30 DEG C ~ 80 DEG C,
Merging filtrate is simultaneously concentrated, and is adsorbed with DM130 macroporous resin column, is successively rinsed with pure water, the ethyl alcohol that mass concentration is 10% ~ 40%,
The ethanol elution for being again 40% ~ 80% with mass concentration collects eluent, extract is obtained after being dried under reduced pressure, wherein cortex cinnamomi is always more
Phenol weight content is 40% ~ 80%.
In the degreasing cinnamon polyphenol extract extracted using the extracting method of above-mentioned degreasing cinnamon polyphenol extract,
Total polyphenols weight content is 40% ~ 80%；Wherein cortex cinnamomi tannin accounts for 30% ~ 55% of total polyphenols weight content in extract；Table catechu
Element accounts for 10% ~ 30% of total polyphenols weight content in extract.
The degreasing cinnamon polyphenol extract that the present invention is extracted from cortex cinnamomi is produced in preparation prevention and treatment diabetes cognition dysfunction
Application in product, including degreasing cinnamon polyphenol extract improve diabetes cognition dysfunction and assist improving diabetes in preparation
Purposes in terms of cognition dysfunction product.
Degreasing cortex cinnamomi polyphenol improves cognition dysfunction, refers to degreasing cortex cinnamomi polyphenol (comprising cortex cinnamomi tannin and cortex cinnamomi table
One of which or two kinds of mixture in catechin) improving studing ability, enhancing brain tissue antioxidant system is active, subtracts
Light brain tissue oxidizing stress damage, it is public to orientation navigation and space exploration incubation period, brain tissue SOD, GSH, MDA and modern medicine
The control for the diabetes cognitive disorder disease index of correlation recognized.
Purposes of the degreasing cinnamon polyphenol extract in terms of preparation improves cognition dysfunction product, refers to degreasing cortex cinnamomi
Polyphenol (including the one of which or two kinds of mixture in cortex cinnamomi tannin and cortex cinnamomi epicatechin), which improves diabetes in preparation, to be recognized
The ability of learning and memory of dysfunction person is demonstrate,proved, neurotrosis and treatment diabetes cognition dysfunction relevant food and drug are mitigated
The purposes of aspect.
The pharmacodynamic experiment of the present inventor shows: degreasing cortex cinnamomi polyphenol is in feeding high in fat+streptozotocin low-dose induction
Diabetes cognition dysfunction rat model discovery, learning cognition ability can be significantly improved for 8 weeks by taking degreasing cortex cinnamomi polyphenol, increase
Add the activity of brain tissue antioxidant system SOD, GSH, and reduce oxidative stress marker MDA, iNOS, NOX, shows that cortex cinnamomi is more
Phenol has protective effect to the brain of diabetes cognition dysfunction rat.
According to the studies above of inventor, the present invention provides degreasing cinnamon polyphenol extract, and there are improvement diabetes to recognize function
The effect of energy obstacle is expected to be used for preventing and treating the diseases such as diabetes cognition dysfunction.
The invention has the following advantages that degreasing cinnamon polyphenol extract extracting method provided by the invention is simple and easy, fit
Suitable industrialized production；Our experiments show that degreasing cortex cinnamomi polyphenol improves ability of learning and memory, SOD, GSH are improved, increases brain tissue and exists
Oxidation resistance in high sugar under environment alleviates brain and causes the effect of oxidativestress damage significant because of high sugar；With fine
Application prospect, and cortex cinnamomi is abundant in china natural resources, and the material residue after industrial abstract cinnamon oil can be used is as degreasing cortex cinnamomi
Raw material sources, low raw-material cost.
Specific embodiment
In order to better illustrate the present invention the characteristics of and essence provide the extraction of degreasing cortex cinnamomi polyphenol in the form of examples below
The extracting method and degreasing cortex cinnamomi polyphenol of object improve the pharmacological results of diabetes cognition dysfunction, illustrate it in pharmacy
Purposes in field.
Embodiment 1
Application of the degreasing cinnamon polyphenol extract in preparation prevention and treatment diabetes cognition dysfunction product, the degreasing cortex cinnamomi
Polyphenol extract includes cortex cinnamomi tannin and one of which or two kinds of mixture in cortex cinnamomi epicatechin, cortex cinnamomi tannin and cortex cinnamomi
The structural formula of epicatechin is as follows:
It is mixed in the mixture of the cortex cinnamomi tannin and both cortex cinnamomi epicatechins with arbitrary proportion.
The extracting method of degreasing cinnamon polyphenol extract is specifically divided into two steps, and respectively, the overcritical degreasing of the first step obtains
Degreasing cortex cinnamomi, second step solvent extraction cinnamon polyphenol extract.The overcritical degreasing of the first step obtains the specific steps of degreasing cortex cinnamomi
It is as follows: to take cassia to crush, be packed into supercritical CO2In extraction kettle, 25 ~ 45MPa of extracting pressure, 30 DEG C ~ 75 DEG C of extraction temperature, point
From I 10 ~ 30MPa of pressure, 25 DEG C ~ 75 DEG C of I temperature are separated, separates 4 ~ 10MPa of II pressure, separates 25 DEG C ~ 55 DEG C of II temperature, CO2
Per kilogram raw material, 2 ~ 10h of extraction take out degreasing cortex cinnamomi to 10 ~ 30kg of flow from extract per hour.Second step solvent extraction meat
Specific step is as follows for osmanthus polyphenol extract: it is 45 ~ 75% that volume fraction, which is added, in 1:1 ~ 1:9 times of weight of degreasing cortex cinnamomi of ratio
Ethyl alcohol extract 1 ~ 3 time until flooding sample completely, extract 1 ~ 10 h every time, temperature is maintained at 30 DEG C ~ 80 DEG C, merges filter
Liquid is simultaneously concentrated, and is adsorbed with DM130 macroporous resin column, is successively rinsed with pure water, the ethyl alcohol that mass concentration is 10% ~ 40%, then is used matter
The ethanol elution that concentration is 40% ~ 80% is measured, eluent is collected, degreasing cinnamon polyphenol extract is obtained after being dried under reduced pressure；It obtains
In degreasing cinnamon polyphenol extract, total polyphenols weight content is 40% ~ 80%.
Embodiment 2
A kind of extracting method of degreasing cinnamon polyphenol extract, is specifically divided into two steps, and respectively, the overcritical degreasing of the first step obtains
Degreasing cortex cinnamomi, second step solvent extraction cinnamon polyphenol extract.The overcritical degreasing of the first step obtains the specific steps of degreasing cortex cinnamomi
It is as follows: to take cassia to crush, be packed into supercritical CO2In extraction kettle, extracting pressure 25MPa, separates I by 30 DEG C ~ 75 DEG C of extraction temperature
Pressure 10MPa separates 25 DEG C ~ 75 DEG C of I temperature, separates II pressure 4MPa, separates 25 DEG C ~ 55 DEG C of II temperature, CO2Flow 10kg is every
Hour per kilogram raw material, extracts 10h, and degreasing cortex cinnamomi is taken out from extract.Second step solvent extraction cinnamon polyphenol extract
Specific step is as follows: the ethyl alcohol that volume fraction is 75% is added in the ratio measured in 1 ~ 9 times of degreasing cortex cinnamomi weight, until flooding sample completely
It until product, extracts 1 time, extracts 10 h every time, temperature is maintained at 30 DEG C ~ 80 DEG C, and merging filtrate is simultaneously concentrated, with DM130 macropore tree
The absorption of rouge column is successively rinsed with pure water, the ethyl alcohol that mass concentration is 10%, then the ethanol elution for being 40% with mass concentration, is collected
Eluent obtains degreasing cinnamon polyphenol extract after being dried under reduced pressure.
Embodiment 3
A kind of extracting method of degreasing cinnamon polyphenol extract, is specifically divided into two steps, and respectively, the overcritical degreasing of the first step obtains
Degreasing cortex cinnamomi, second step solvent extraction cinnamon polyphenol extract.The overcritical degreasing of the first step obtains the specific steps of degreasing cortex cinnamomi
It is as follows: to take cassia to crush, be packed into supercritical CO2In extraction kettle, extracting pressure 35MPa, separates I by 30 DEG C ~ 75 DEG C of extraction temperature
Pressure 20MPa separates 25 DEG C ~ 75 DEG C of I temperature, separates II pressure 6MPa, separates 25 DEG C ~ 55 DEG C of II temperature, CO2Flow 20kg is every
Hour per kilogram raw material, extracts 8h, and degreasing cortex cinnamomi is taken out from extract.The tool of second step solvent extraction cinnamon polyphenol extract
Steps are as follows for body: the ethyl alcohol that volume fraction is 60% is added in the ratio measured in 6 times of degreasing cortex cinnamomi weight, extracts 2 times, extracts every time
7h, temperature are maintained at 30 DEG C ~ 80 DEG C, and merging filtrate is simultaneously concentrated, and are adsorbed with DM130 macroporous resin column, successively dense with pure water, quality
Degree rinses for 30% ethyl alcohol, then the ethanol elution for being 60% with mass concentration, collects eluent, obtains defatted meat after being dried under reduced pressure
Osmanthus polyphenol extract.
Embodiment 4
A kind of extracting method of degreasing cinnamon polyphenol extract, is specifically divided into two steps, and respectively, the overcritical degreasing of the first step obtains
Degreasing cortex cinnamomi, second step solvent extraction cinnamon polyphenol extract.The overcritical degreasing of the first step obtains the specific steps of degreasing cortex cinnamomi
It is as follows: to take cassia to crush, be packed into supercritical CO2In extraction kettle, extracting pressure 45MPa, separates I by 30 DEG C ~ 75 DEG C of extraction temperature
Pressure 30MPa separates 25 DEG C ~ 75 DEG C of I temperature, separates II pressure 10MPa, separates 25 DEG C ~ 55 DEG C of II temperature, CO2Flow 30kg
Per kilogram raw material per hour extracts 2h, and degreasing cortex cinnamomi is taken out from extract.Second step solvent extraction cinnamon polyphenol extract
Specific step is as follows: the ethyl alcohol that volume fraction is 45% is added in the ratio measured in 9 times of degreasing cortex cinnamomi weight, extracts 3 times, mentions every time
1 h is taken, temperature is maintained at 30 DEG C ~ 80 DEG C, and merging filtrate is simultaneously concentrated, and is adsorbed with DM130 macroporous resin column, successively uses pure water, matter
It measures the ethyl alcohol that concentration is 40% to rinse, then the ethanol elution for being 80% with mass concentration, collects eluent, taken off after being dried under reduced pressure
Rouge cinnamon polyphenol extract.
Embodiment 5
Cognitive function protective effect of the degreasing cortex cinnamomi polyphenol to diabetes cognition dysfunction rat
1.1 reagents: degreasing cortex cinnamomi polyphenol: using cortex cinnamomi as raw material, according to the extraction side of 3 degreasing cinnamon polyphenol extract of embodiment
Method extracts resulting degreasing cinnamon polyphenol extract.Streptozotocin (STZ), SIGMA Products；Donepezil piece, Jiangsu
Hao Sen medicine company Group Co., Ltd；Blood sugar test paper, the production of product (Shanghai) Co., Ltd., Roche Diagnistics；High lipid food formula: base
Plinth feed 74.5%, cholesterol 1%, yolk powder 5%, sucrose 10%, lard 9%, sodium taurocholate 0.5%；Superoxide dismutase (SOD) is surveyed
Determine kit, micro malonaldehyde (MDA) testing cassete, micro reduced glutathione testing cassete, nadh oxidase kit, an oxygen
Change nitrogen synthase (NOS) assay kit, Bioengineering Research Institute's production is built up in Nanjing.
1.2 experimental animals: it is limited up to experimental animal to be purchased from Hunan Si Laike scape by SD rat, all-male, weight 180-220 g
Company.
1.3 experimental methods: taking SD male rat several, feeding high lipid food, separately sets blank group, feeds normal diet.6
Go out all 35 mg/kg modelings of rats by intraperitoneal injection streptozotocin outside blank group after week, blank group injects the citrate of equivalent
Buffer, 72 h survey 1 blood glucose after injecting streptozotocin, and blood glucose > 11.1 mmol/L then think modeling success.By modeling at
The rat of function is uniformly divided into 5 groups according to blood glucose value at random: model group, degreasing cortex cinnamomi polyphenol high (400 mg/kg), in (200 mg/
Kg), low (100 mg/kg) three dosage groups, donepezil group (0.07 mg/kg).Administration group ig administration, 1 times/day, blank group
It fills with model group with equivalent pure water, continuous gavage 8 weeks, blank group was fed and controlled with normal diet, model group and each drug during experiment
Treatment group continues to give high lipid food nursing.
During administration, in 2,4,6,8 weeks measurement fasting blood sugars of administration.The 8th week progress Morris water maze laboratory is administered,
Investigate animal learning cognitive ability.After being administered 8 weeks, after 7% chloral hydrate anesthesia, broken end takes brain tissue after bloodletting, for detecting
SOD, MDA, GSH, iNOS and NOX.
1.4 statistical analysis: experimental result is indicated with mean+SD.Using each group difference of t check analysis.
1.5 experimental results: during drug treatment, model group fasting blood sugar maintains always higher level.Drug treatment
From 4 weeks, compared with model group, there is significant hypoglycemic effect (P < 0.05), and the effect in degreasing cortex cinnamomi polyphenol high dose
Continue to the 8th week (P < 0.05, compared with model group)；After being administered 6 weeks, degreasing cortex cinnamomi polyphenol middle dosage and low dosage occur
Significant hypoglycemic effect (P < 0.05), after also continueing to administration 8 weeks.Show that degreasing cortex cinnamomi polyphenol is big to diabetic nephropathy
Mouse has stable hypoglycemic effect.Data are shown in Table one.
In diabetes cognition dysfunction rat in Morris water maze test, compared with model group, donepezil and
Degreasing cortex cinnamomi polyphenol 400mg/kg, 200mg/kg, 100mg/kg group learning ability level is obviously improved (P < 0.01 or P < 0.05).
In tests in 5 days of Morris water maze study, the escape latency of donepezil and degreasing cortex cinnamomi polyphenol administration group rat exists
Shorten with the increase of training number of days in training, until at the 5th day, donepezil and degreasing cortex cinnamomi polyphenol 400,200,
The equal escape latency of 100mg/kg group significantly shortens (compared with model group, P < 0.01).Show degreasing cortex cinnamomi polyphenol to sugar
Urinating sick cognition dysfunction rat plays the role of improving studing ability.Data are shown in Table two.
At the water maze laboratory third day Morris, degreasing cortex cinnamomi polyphenol 200,100mg/kg group animal are in platform quadrant
Movable percentage has been significantly higher than model group (P < 0.01), donepezil group and degreasing cortex cinnamomi polyphenol at the 4th day and the 5th day
400,200,100mg/kg group is all remarkably higher than model group (P < 0.01) in platform quadrant activity percentage.Show that degreasing cortex cinnamomi is more
Phenol facilitates the promotion of diabetes cognition dysfunction learning and memory in rats ability.Data are shown in Table three.
Morris water maze laboratory last day removes platform and carries out space exploration experiment.Compared with model group, mostly how piperazine
Neat group and degreasing cortex cinnamomi polyphenol 400mg/kg, 200mg/kg, 100mg/kg group spanning platform number significantly increase (P < 0.01).Table
The treatment of bright degreasing cortex cinnamomi polyphenol, can be effectively improved diabetes rat cognition dysfunction, have enhancing space exploration ability, mitigate
The effect of cognitive function decline.It is shown in Table four.
In order to further verify the protective effect to brain tissue of degreasing cortex cinnamomi polyphenol, we have detected the brain of groups of animals
Tissue oxidizing stress index of correlation.Compared with model group, degreasing cortex cinnamomi polyphenol 100mg/kg group and 400mg/kg group rat brain group
Knit SOD vigor, GSH content significantly improves (P < 0.05 or P < 0.01), while MDA content significantly reduce (P < 0.01).Furthermore it takes off
Rouge cortex cinnamomi polyphenol 200mg/kg group Brain Tissue SOD Vigor, GSH content are also in obviously increase trend.Show in degreasing cortex cinnamomi polyphenol
After treatment, diabetes rat brain tissue oxidation resistance can be effectively improved, protection body is from because of the nerve caused by oxidative stress
Damage.Data are shown in Table five.
In the brain tissue of diabetes cognition dysfunction rat, degreasing cortex cinnamomi polyphenol 400,200, the iNOS of 100mg/kg group it is living
Property and amount of NOx significantly reduce (P < 0.01).Show to control diabetes cognition dysfunction rat using degreasing cortex cinnamomi polyphenol
Amount of nitrogen oxides can be effectively reduced in treatment, mitigates nitrogen oxides and damages caused by brain tissue.Data are shown in Table six.
1.6 conclusions: the experiment discovery of people, degreasing cortex cinnamomi polyphenol can significantly alleviate diabetes cognitive function through the invention
The fasting blood-glucose of obstacle rat increases, while protecting the space exploration ability and ability of learning and memory of diabetes rat, and significant
Increase the content of SOD, GSH polyphenoils, at the same reduce MDA, iNOS, NOX etc. stress marker level, show degreasing cortex cinnamomi
Polyphenol can alleviate cognitive disorder and the neurotrosis of diabetes cognition dysfunction rat, and having improves diabetes cognition dysfunction
Effect.
Claims (7)
1. application of the degreasing cinnamon polyphenol extract in preparation prevention and treatment diabetes cognition dysfunction product.
2. degreasing cinnamon polyphenol extract according to claim 1 is in preparation prevention and treatment diabetes cognition dysfunction product
Application, it is characterised in that: the degreasing cinnamon polyphenol extract preparation improve diabetes cognition dysfunction nerve damage
Injure prevention diabetes cognition dysfunction relevant food and drug in terms of purposes.
3. degreasing cinnamon polyphenol extract according to claim 1 or 2 is produced in preparation prevention and treatment diabetes cognition dysfunction
Application in product, it is characterised in that: the degreasing cinnamon polyphenol extract includes in cortex cinnamomi tannin and cortex cinnamomi epicatechin
The structural formula of one of which or two kinds of mixture, cortex cinnamomi tannin and cortex cinnamomi epicatechin is as follows:
It is mixed in the mixture of the cortex cinnamomi tannin and both cortex cinnamomi epicatechins with arbitrary proportion.
4. degreasing cinnamon polyphenol extract according to claim 3 is in preparation prevention and treatment diabetes cognition dysfunction product
Application, it is characterised in that: the extracting method of the degreasing cinnamon polyphenol extract is divided into two steps, and respectively, the first step is super
Critical degreasing obtains degreasing cortex cinnamomi, second step solvent extraction cinnamon polyphenol extract.
5. degreasing cinnamon polyphenol extract according to claim 4 is in preparation prevention and treatment diabetes cognition dysfunction product
Application, it is characterised in that: in the extracting method of the degreasing cinnamon polyphenol extract, the overcritical degreasing of the first step is taken off
Specific step is as follows for rouge cortex cinnamomi: taking cassia to crush, is packed into supercritical CO2In extraction kettle, 25 ~ 45MPa of extracting pressure, extraction
30 DEG C ~ 75 DEG C of temperature, 10 ~ 30MPa of I pressure is separated, separates 25 DEG C ~ 75 DEG C of I temperature, separates 4 ~ 10MPa of II pressure, separates II temperature
25 DEG C ~ 55 DEG C of degree, CO2Per kilogram raw material, 2 ~ 10h of extraction take out degreasing cortex cinnamomi to 10 ~ 30kg of flow from extract per hour.
6. degreasing cinnamon polyphenol extract according to claim 4 is in preparation prevention and treatment diabetes cognition dysfunction product
Application, it is characterised in that: in the extracting method of the degreasing cinnamon polyphenol extract, second step solvent extraction cortex cinnamomi polyphenol
Specific step is as follows for extract: the ethyl alcohol that volume fraction is 45 ~ 75% is added in 1:1 ~ 1:9 times of weight of degreasing cortex cinnamomi of ratio,
Until flooding sample completely, extracts 1 ~ 3 time, extract 1 ~ 10 h every time, temperature is maintained at 30 DEG C ~ 80 DEG C, and merging filtrate is simultaneously dense
Contracting is adsorbed with DM130 macroporous resin column, is successively rinsed with pure water, the ethyl alcohol that mass concentration is 10% ~ 40%, then is used mass concentration
For 40% ~ 80% ethanol elution, eluent is collected, extract is obtained after being dried under reduced pressure, wherein cinnamon total polyphenol weight content is
40%~80%。
7. degreasing cinnamon polyphenol extract according to claim 4 is in preparation prevention and treatment diabetes cognition dysfunction product
Application, it is characterised in that: it is more using the degreasing cortex cinnamomi extracted of extracting method of the degreasing cinnamon polyphenol extract
In phenol extraction object, total polyphenols weight content is 40% ~ 80%；Wherein cortex cinnamomi tannin account for total polyphenols weight content in extract 30% ~
55%；Epicatechin accounts for 10% ~ 30% of total polyphenols weight content in extract.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910227299.7A CN109966285B (en) | 2019-03-25 | 2019-03-25 | Application of degreased cinnamon polyphenol extract in preparation of products for preventing and treating diabetes cognitive dysfunction |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910227299.7A CN109966285B (en) | 2019-03-25 | 2019-03-25 | Application of degreased cinnamon polyphenol extract in preparation of products for preventing and treating diabetes cognitive dysfunction |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109966285A true CN109966285A (en) | 2019-07-05 |
| CN109966285B CN109966285B (en) | 2021-09-21 |
Family
ID=67080295
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910227299.7A Active CN109966285B (en) | 2019-03-25 | 2019-03-25 | Application of degreased cinnamon polyphenol extract in preparation of products for preventing and treating diabetes cognitive dysfunction |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109966285B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112891394A (en) * | 2021-03-02 | 2021-06-04 | 广西壮族自治区中医药研究院 | Application of cinnamon extract in preparation of hypoglycemic drugs |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8895083B2 (en) * | 2008-10-28 | 2014-11-25 | Allegiance Equity Corporation | Composition for the maintenance of blood sugar levels comprising cinnamon and ginseng |
-
2019
- 2019-03-25 CN CN201910227299.7A patent/CN109966285B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8895083B2 (en) * | 2008-10-28 | 2014-11-25 | Allegiance Equity Corporation | Composition for the maintenance of blood sugar levels comprising cinnamon and ginseng |
Non-Patent Citations (2)
| Title |
|---|
| AZADEH MESRIPOUR等: "The effect of Cinnamomum zeylanicum bark water extract on memory performance in alloxan-induced diabetic mice", 《RESEARCH IN PHARMACEUTICAL SCIENCES》 * |
| 李英奇等: "脱脂肉桂粉提取肉桂多酚的研究", 《中草药》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112891394A (en) * | 2021-03-02 | 2021-06-04 | 广西壮族自治区中医药研究院 | Application of cinnamon extract in preparation of hypoglycemic drugs |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109966285B (en) | 2021-09-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Luo et al. | [Retracted] Ginseng on Hyperglycemia: Effects and Mechanisms | |
| Adzu et al. | Evaluation of the antidiarrhoeal effects of Zizyphus spina-christi stem bark in rats | |
| Moradabadi et al. | Hypoglycemic effects of three medicinal plants in experimental diabetes: Inhibition of rat intestinal α-glucosidase and enhanced pancreatic Insulin and cardiac Glut-4 mRNAs expression | |
| Mohammadi et al. | The effect of hydro alcoholic extract of Juglans regia leaves in streptozotocin-nicotinamide induced diabetic rats | |
| Aja et al. | Evaluation of anti-diabetic effect and liver enzymes activity of ethanol extract of Pterocarpus santalinoides in alloxan induced diabetic albino rats | |
| Akter et al. | Comparative studies on antidiabetic effect with phytochemical screening of Azadirachta indicia and Andrographis paniculata | |
| Zaid et al. | State of the art of diabetes treatment in Greco-Arab and islamic medicine | |
| Shrestha et al. | A review on Nepalese medicinal plants used traditionally as alpha-amylase and alpha-glucosidase inhibitors against diabetes mellitus | |
| Egua et al. | Antidiabetic potential of liquid-liquid partition fractions of ethanolic seed extract of Corchorus olitorious | |
| Ko et al. | Ameliorative effect of Ruellia tuberosa L. on hyperglycemia in type 2 diabetes mellitus and glucose uptake in mouse C2C12 myoblasts | |
| Nnamdi et al. | Hypoglycemic effects of aqueous and ethanolic extracts of dandelion (Taraxacum officinale FH Wigg.) leaves and roots on streptozotocin-induced albino rats | |
| CN109966285A (en) | Application of the degreasing cinnamon polyphenol extract in preparation prevention and treatment diabetes cognition dysfunction product | |
| Nayak et al. | Antidiabetic activity of aerial parts of Argemone mexicana Linn. in alloxan induced hyperglycaemic rats | |
| SIa et al. | Antidiabetic and hepatoprotective effects of combined aqueous leaf extract of Azadirachta indica and Mangifera indica in alloxan monohydrate-induced diabetic Wistar Rats | |
| Widayanti et al. | Antidiabetics activity of salam koja (Murraya koenigii) leaves tea bag | |
| Pokale et al. | Piper longum (Linn.) restores ovarian function in Letrozole induced PCOS in Rats: Comparison with Metformin and Clomiphene citrate | |
| CN106822166A (en) | A kind of medicine and its application in pharmacy for preventing and treating diabetes and hyperlipidemia | |
| Lee et al. | 2-Hydroxyquinoline and its structural analogs show antidiabetic effects against α-amylase and α-glucosidase | |
| Elrherabi et al. | Antihyperglycemic potential of the Lavandula stoechas aqueous extract via inhibition of digestive enzymes and reduction of intestinal glucose absorption | |
| Tsegaye et al. | Antidiabetic activity of samma (Urtica simensis hochst. Ex. A. Rich.) in streptozotocin-induced diabetic mice | |
| Rout et al. | Hypoglycaemic activity of aerial parts of Argemone mexicana L. in experimental rat models | |
| Umar et al. | In vivo antihyperglycaemic activity of crude and partitioned fractions of selected medicinal plants | |
| KR20090091615A (en) | Tetracera scandens extracts and 4h-chromen-4-one derivatives isolated therefrom increasing glucose uptake in differentiated l6 muscle cells | |
| Chinedu et al. | Acute administration of aqueous extract of Garcinia kola on daily blood glucose level and selected biochemical indices in longevity wistar albino rats | |
| Nayak et al. | Evaluation of antidiabetic and antioxidant activity of aerial parts of Hyptis suaveolens Poit |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |