CN109964936A - For reducing the regulator and its method of Nitrate Accumulation in burley tobaccos - Google Patents

For reducing the regulator and its method of Nitrate Accumulation in burley tobaccos Download PDF

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Publication number
CN109964936A
CN109964936A CN201910384346.9A CN201910384346A CN109964936A CN 109964936 A CN109964936 A CN 109964936A CN 201910384346 A CN201910384346 A CN 201910384346A CN 109964936 A CN109964936 A CN 109964936A
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burley tobaccos
regulator
tobacco leaf
auxin
glycerine
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Chinese (zh)
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史宏志
冯雨晴
杨惠娟
段卫东
王景
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Henan Agricultural University
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Henan Agricultural University
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00—Cultivation of specific crops or plants not otherwise provided for
    • A01G22/45—Tobacco
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/02—Acyclic compounds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
    • A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Zoology (AREA)
  • Agronomy & Crop Science (AREA)
  • Botany (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Chemical & Material Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The present invention relates to a kind of regulator and its method for reducing Nitrate Accumulation in burley tobaccos, the weight percent of each component are as follows: glycerine 0.1 ~ 2%；Auxin 0.0005 ~ 0.002%；Sodium molybdate 0.1 ~ 2%；Chitosan oligosaccharide 0.01 ~ 0.02%, surplus are water.The present invention is to spray regulator to cigarette strain respectively in group phase, prosperous growth phase and the maturity period of burley tobaccos growth, the regulator enhances the carbon fixation ability of burley tobaccos by glycerine and chitosan oligosaccharide, promote carbon skeleton synthesis, improves the activity of leaf photosynthesis and nitrate reductase；Enhance burley tobaccos nitrogen by sodium molybdate and restores assimilative capacicy；Promote the increase of burley tobaccos cell division and blade cell quantity by auxin, thus achieve the purpose that reduce Nitrate Accumulation in burley tobaccos, it is final to realize the purpose for reducing content of nitrosamines in tobacco leaf.

Description

For reducing the regulator and its method of Nitrate Accumulation in burley tobaccos
Technical field:
Tobacco is reduced the invention belongs to improving cigarette quality, technical field is damaged to human health, and in particular to it is a kind of for reducing The regulator and its method of Nitrate Accumulation in burley tobaccos.
Background technique:
Tobacco-specific nitrosamine (TSNA) mainly includes N- nitrosonornicotine (NNN), 4- (N- methyl-nitrosamine) -1- 4 kinds of (3- pyridyl group) -1- butanone (NNK), N- nitrosoanatabine (NAT) and N- nitroso anabasine (NAB), by Confirm related with generations of region tumors such as lung, oral cavity, esophagus, pancreas, livers.
Many studies have shown that there's almost no TSNA in fresh tobacco leaves, being formed and accumulating mainly in the modulation of tobacco In storage.Nitrate is the precursor that tobacco-specific nitrosamine is formed, and contents level is the important finger of TSNAs accumulation Show object.Nitrate nitrogen is the Main Morphology of plant absorption nitrogen, restores assimilation by nitrogen and is absorbed and utilized by plant, and cigarette Leaf is housed in library after harvesting, then the nitrate nitrogen in tobacco leaf accumulates in tobacco leaf cells, it is difficult to which assimilate reduction, to lead Cause tobacco after modulation and storage, the content of nitrosamines in tobacco significantly improves.
Burley tobaccos are the important source materials of blended type cigarette, studies have shown that burley tobaccos are a kind of minus green mutant, having needs nitrogen The high feature low with nitrogen utilization efficiency of amount, and the study found that burley tobaccos nitrogen utilization efficiency is low, it is straight to be that its nitrate largely accumulates Reason is connect, and the pigment content of burley tobaccos is low, photosynthesis and carbon fixation ability are weak so as to cause cannot be its nitrogen metabolism process Sufficient energy is provided, is the main reason for its nitrate largely accumulates.That is Nitrate content is very in burley tobaccos Greatly.
And burley tobaccos category air-curing of tobacco leaves, the modulation of burley tobaccos must carry out in room drying in the air, and make tobacco leaf dehydration using cross-ventilation, become Color, drying, internal substance convert simultaneously.Modulator approach is until hanging over Mature Tobacco Leaves and drying in dry in the air room or the canopy that dries in the air.And white rib During drying in the air processed, tobacco leaf cells dehydration rupture causes nitrate species constantly to leak, forms nitrous by microbial action cigarette Amine, therefore, the study found that the content of the nitrate after modulation in burley tobaccos is ten to the hundreds times of Nitrate in Flue-cured Tobacco content, This is also to cause the reason of content of nitrosamines is significantly larger than content of nitrosamines in flue-cured tobacco in burley tobaccos.
It therefore, can be effective the study found that reducing accumulation of the nitrate in tobacco cell in burley tobaccos growth course The formation of nitrosamine in tobacco leaf after modulating is reduced, to achieve the purpose that reduce content of nitrosamines in tobacco leaf.Currently, China part Tobacco seed growing area is by adjusting the dosage either forms of nitrogen of nitrogenous fertilizer, and by increasing the p application rate, applying the measures such as hormone reduces cigarette Leaf Nitrate Accumulation, still, for burley tobaccos, burley tobaccos have the characteristics that nitrogen requirement height and nitrogen utilization efficiency are low, therefore, The method of existing adjustment nitrogen fertilizer amount is not appropriate for burley tobaccos.
Summary of the invention:
In conclusion the present invention provides one kind in order to overcome the shortcomings of prior art problem for reducing nitric acid in burley tobaccos The regulator and its method of salt accumulation, it was sprayed respectively to cigarette strain in group phase, prosperous growth phase and the maturity period of burley tobaccos growth Regulator, the regulator enhance the carbon fixation ability of burley tobaccos by glycerine and chitosan oligosaccharide, promote carbon skeleton synthesis, improve leaf The activity of piece photosynthesis and nitrate reductase；Enhance burley tobaccos nitrogen by sodium molybdate and restores assimilative capacicy；Pass through auxin Promote the increase of burley tobaccos cell division and blade cell quantity, thus achieve the purpose that reduce Nitrate Accumulation in burley tobaccos, It is final to realize the purpose for reducing content of nitrosamines in tobacco leaf.
In order to solve the above technical problems, the technical scheme of the present invention is realized as follows:
A kind of regulator for reducing Nitrate Accumulation in burley tobaccos, in which: the weight percent of each component are as follows: glycerine 0.1~2%；Auxin 0.0005 ~ 0.002%；Sodium molybdate 0.1 ~ 2%；Chitosan oligosaccharide 0.01 ~ 0.02%, surplus are water.
Technical solution of the present invention may also is that realization: the weight percent of each component are as follows: glycerine 1%；Growth Element 0.001%；Sodium molybdate 1%；Chitosan oligosaccharide 0.015%, surplus are water.
Technical solution of the present invention may also is that realization: the auxin is heteroauxin.
A method of Nitrate Accumulation in burley tobaccos is reduced using above-mentioned regulator, in which: is comprised the following steps that:
A, configuration adjustment agent:
Glycerine, auxin, sodium molybdate and chitosan oligosaccharide are weighed by weight percentage, and each component of weighing is mixed with water It is even；
B, in the group phase of tobacco leaf growth, configured regulator is sprayed to cigarette strain；
C, in the prosperous growth phase of tobacco leaf growth, configured regulator is sprayed to cigarette strain；
D, in the tobacco leaf maturity period, configured regulator is sprayed to cigarette strain again.
Technical solution of the present invention may also is that realization: in step a, the weight percent of each component of weighing Are as follows: glycerine 0.1 ~ 2%；Auxin 0.0005 ~ 0.002%；Sodium molybdate 0.1 ~ 2%；Chitosan oligosaccharide 0.01 ~ 0.02%, surplus are water.
Technical solution of the present invention may also is that realization: in step a, the weight percent of each component of weighing Are as follows: glycerine 1%；Auxin 0.001%；Sodium molybdate 1%；Chitosan oligosaccharide 0.0105%, surplus are water.
Technical solution of the present invention may also is that realization: in step b, the group phase of the tobacco leaf growth is cigarette 25-30 days behind transplantation of seedlings crop field, when group's phase sprays regulator to cigarette strain, sprayed in fine day 4-6 in afternoon point, tobacco leaf is positive and negative Face uniformly sprays, until the solution on tobacco leaf forms uniform drop.
Technical solution of the present invention may also is that realization: in step c, the prosperous growth phase of the tobacco leaf growth is cigarette 35-45 days behind transplantation of seedlings crop field.
Technical solution of the present invention may also is that realization: in step d, the tobacco leaf maturity period is cigarette strain topping 10 ~ 15 days afterwards.
Technical solution of the present invention may also is that realization: in step c and step d, the regulator is sprayed Amount is 40-50Kg/ mus.
The invention has the benefit that
1, the present invention is to spray regulator to cigarette strain respectively in group phase, prosperous growth phase and the maturity period of burley tobaccos plantation, the adjusting Agent enhances the carbon fixation ability of burley tobaccos by glycerine and chitosan oligosaccharide, promotes carbon skeleton synthesis, improve leaf photosynthesis and The activity of nitrate reductase；Enhance burley tobaccos nitrogen by sodium molybdate and restores assimilative capacicy；Promote burley tobaccos thin by auxin The increase of born of the same parents division and blade cell quantity, to achieve the purpose that reduce Nitrate Accumulation in burley tobaccos, final realize is reduced The purpose of content of nitrosamines in tobacco leaf.
2, containing glycerine and chitosan oligosaccharide in regulator of the invention, glycerine and chitosan oligosaccharide itself are used as a kind of carbon source, Carbon skeleton can be provided for tobacco leaf nitrogen metabolism process, can be improved pigment in plant leaf to the capture energy of II luminous energy of light and system PS Power, improves photosynthesis and increase carbohydrate is formed, and provides enough energy and energy substance for N in Tobacco metabolic process, because This, glycerine and chitosan oligosaccharide can promote carbon skeleton synthesis aspect, reduce nitrate in tobacco leaf from enhancing tobacco carbon fixation ability Content, improve quality of tobacco.
3, contain sodium molybdate in regulator of the invention, molybdenum is the important component of nitrate reductase, and nitrate reductase is Key enzyme in nitrate reduction assimilation process, nitrate reductase play key effect during tobacco nitrogen absorption and utilization, Therefore, the content of molybdenum directly affects N in Tobacco metabolic process in tobacco leaf, and sodium molybdate is contained in regulator of the invention, sodium molybdate The content that can effectively improve molybdenum element in tobacco leaf is added, so that the activity of nitrate reductase is effectively improved, to accelerate The nitrogen metabolism of tobacco improves utilization rate of nitrogen fertilizer, to reduce the content of nitrate in tobacco leaf.The present invention select sodium molybdate rather than Ammonium molybdate, that is because ammonium molybdate itself contains NH4 +, NH4 +It is the product of nitrate assimilation, external source NH4 +Addition can reduce paddy Glutamine synzyme (GS) activity reduces, to block nitrogen assimilation process, reduces organic nitrogen content, it is same to be unfavorable for nitrate reduction The progress of change process.In addition, the study found that sodium molybdate can also improve pigment content in tobacco leaf, photosynthetic rate, two sugared contents, Nitrate reductase activity and content of soluble protein, tobacco leaf biomass are significantly increased.
4, auxin of the invention uses heteroauxin, and tobacco cell can be promoted to carry out division and blade cell quantity Increase, as important signaling molecule, auxin participates in many processes of plant growth, development, promote tobacco metabolism, into And the leaf area of tobacco leaf is promoted to expand, achieve the effect that promote cigarette strain growth, improves yield of tobacco, therefore, the addition of auxin It can speed up C N metabolism when tobacco growing, to reduce the accumulation of nitrate in tobacco leaf, tobacco C N metabolism activity is main Carry out Comprehensive Assessment by Leaf biomass.
5, the method and step for reducing Nitrate Accumulation in burley tobaccos of the invention is simple, and operation is easy, easy to promote and utilize, Low in cost, effect is good, quick, can effectively reduce the content of the nitrosamine after burley tobacco leaf is modulated in tobacco leaf, be promoted Quality of tobacco reduces harmfulness of smoking.
Specific embodiment
Below with reference to embodiment, the present invention is described in further detail.
Embodiment one:
A kind of regulator for reducing Nitrate Accumulation in burley tobaccos, the weight percent of each component are as follows: glycerine 0.1%；It is raw Long element 0.0005%；Sodium molybdate 0.1%；Chitosan oligosaccharide 0.01%, surplus are water.The auxin is heteroauxin.
A method of Nitrate Accumulation in burley tobaccos is reduced using above-mentioned regulator, in which: is comprised the following steps that:
A, configuration adjustment agent:
Glycerine 0.1% is weighed by weight percentage；Auxin 0.0005%；Sodium molybdate 0.1%；Chitosan oligosaccharide 0.01%, and will weigh Each component be uniformly mixed with water；
B, in the group phase of tobacco leaf growth, configured regulator is sprayed to cigarette strain；
The group phase of the tobacco leaf growth is that tobacco seedlings are transplanted behind crop field 25-30 days, when group's phase sprays regulator to cigarette strain, Spray in the at 5 points in the afternoon of fine day, and tobacco leaf front and back sides uniformly spray, and is up to the solution on tobacco leaf forms uniform drop Only.It sprays the evaporation that can effectively reduce regulator at 5 points in the afternoon of fine day, guarantees that regulator is absorbed and utilized by blade.
C, in the prosperous growth phase of tobacco leaf growth, configured regulator is sprayed to cigarette strain；
The prosperous growth phase of the tobacco leaf growth is that tobacco seedlings are transplanted behind crop field 35-45 days, and the amount of spraying of the regulator is 45Kg/ Mu
D, in the tobacco leaf maturity period, configured regulator is sprayed to cigarette strain again.
The tobacco leaf maturity period is 10 ~ 15 days after cigarette strain topping.The amount of spraying of the regulator is 45Kg/ mus.
Embodiment two:
Embodiment one is repeated, there is following difference: a kind of regulator for reducing Nitrate Accumulation in burley tobaccos, each component Weight percent are as follows: glycerine 1%；Auxin 0.001%；Sodium molybdate 1%；Chitosan oligosaccharide 0.015%, surplus are water.The growth Element is heteroauxin.
Embodiment three:
Embodiment one is repeated, there is following difference: a kind of regulator for reducing Nitrate Accumulation in burley tobaccos, each component Weight percent are as follows: glycerine 2%；Auxin 0.002%；Sodium molybdate 2%；Chitosan oligosaccharide 0.02%, surplus are water.The auxin For heteroauxin.
Comparative example one:
Repeat embodiment one, there is following difference: the regulator selection clear water divides according to the spraying method of embodiment one Clear water is not sprayed in group phase, prosperous growth phase and the maturity period of tobacco leaf growth.
Comparative example two:
Repeat embodiment one, there is following difference: the weight ratio of the regulator selection glycerine, glycerine and water is 1: 100, spraying method is identical as the spraying method of embodiment one.
Comparative example three:
Repeat embodiment one, there is following difference: the regulator selection glycerine and auxin, auxin are indoles second Acid, weight percentage of each component are glycerine 1%, auxin 0.001%, the spraying method phase of spraying method and embodiment one Together.
Comparative example four:
Repeat embodiment one, there is following difference: the regulator is sodium molybdate, and the weight ratio of sodium molybdate and water is 1:100, Its spraying method is identical as the spraying method of embodiment one.
After tobacco leaf is mature, embodiment one, embodiment two, embodiment three, comparative example one, comparative example are picked respectively Two, the burley tobaccos blade of comparative example three-level comparative example four carries out net photosynthesis speed to the burley tobaccos blade of picking respectively Rate, nitrate reductase activity, Leaf biomass and nitrate content analysis, the results are shown in Table 1 for analysis
Table 1
As can be seen from Table 1, comparative example two, three, four can be improved burley tobaccos growth course relative to comparative example one In photosynthetic efficiency, improve the activity of nitrate reductase, improve the biomass of burley tobaccos blade, white rib can be effectively reduced The content of nitrate in Tobacco Leaves, and use the embodiment one, two, three after regulator of the invention and implement relative to comparison Example, can significantly improve the photosynthetic photosynthetic efficiency in burley tobaccos growth course, hence it is evident that the activity of nitrate reductase is improved, it is bright The aobvious biomass for improving burley tobaccos blade, can effectively reduce the content of nitrate in burley tobaccos blade, especially embodiment Regulator used in two, embodiment two is compared with comparative example one, and Net Photosynthetic Rate improves 68.91%, nitrate reductase Activity improve 53.09%, the biomass of burley tobaccos blade improves 44.63%, and the content of nitrate reduces in burley tobaccos blade 40.62%。

Claims (10)

1. a kind of regulator for reducing Nitrate Accumulation in burley tobaccos, in which: the weight percent of each component are as follows: glycerine 0.1~2%；Auxin 0.0005 ~ 0.002%；Sodium molybdate 0.1 ~ 2%；Chitosan oligosaccharide 0.01 ~ 0.02%, surplus are water.
2. the regulator according to claim 1 for reducing Nitrate Accumulation in burley tobaccos, feature with each component Weight percent are as follows: glycerine 1%；Auxin 0.001%；Sodium molybdate 1%；Chitosan oligosaccharide 0.015%, surplus are water.
3. the regulator according to claim 1 for reducing Nitrate Accumulation in burley tobaccos, feature with: it is described Auxin is heteroauxin.
4. utilizing the method for reducing Nitrate Accumulation in burley tobaccos such as the described in any item regulators of claim 1 ~ 3, in which: packet Include following processing step:
A, configuration adjustment agent:
Glycerine, auxin, sodium molybdate and chitosan oligosaccharide are weighed by weight percentage, and each component of weighing is mixed with water It is even；
B, in the group phase of tobacco leaf growth, configured regulator is sprayed to cigarette strain；
C, in the prosperous growth phase of tobacco leaf growth, configured regulator is sprayed to cigarette strain；
D, in the tobacco leaf maturity period, configured regulator is sprayed to cigarette strain again.
5. the method according to claim 4 for reducing Nitrate Accumulation in burley tobaccos, it is characterised in that: in step a, weigh Each component weight percent are as follows: glycerine 0.1 ~ 2%；Auxin 0.0005 ~ 0.002%；Sodium molybdate 0.1 ~ 2%；Chitosan oligosaccharide 0.01 ~ 0.02%, surplus is water.
6. the method according to claim 4 for reducing Nitrate Accumulation in burley tobaccos, it is characterised in that: in step a, weigh Each component weight percent are as follows: glycerine 1%；Auxin 0.001%；Sodium molybdate 1%；Chitosan oligosaccharide 0.0105%, surplus are water.
7. the method according to claim 4 for reducing Nitrate Accumulation in burley tobaccos, it is characterised in that: described in step b Group phase of tobacco leaf growth be that tobacco seedlings are transplanted behind crop field 25-30 days, a phase is rolled into a ball when spraying regulator to cigarette strain, in fine day afternoon 4 ~ 6 points spray, and tobacco leaf front and back sides uniformly spray, until the solution on tobacco leaf forms uniform drop.
8. the method according to claim 4 for reducing Nitrate Accumulation in burley tobaccos, it is characterised in that: described in step c Tobacco leaf growth the prosperous growth phase be tobacco seedlings transplant crop field after 35-45 days.
9. the method according to claim 4 for reducing Nitrate Accumulation in burley tobaccos, it is characterised in that: described in step d The tobacco leaf maturity period be cigarette strain topping after 10 ~ 15 days.
10. the method according to claim 4 for reducing Nitrate Accumulation in burley tobaccos, it is characterised in that: step c and step In d, the amount of spraying of the regulator is 40-50Kg/ mus.
CN201910384346.9A 2019-05-09 2019-05-09 For reducing the regulator and its method of Nitrate Accumulation in burley tobaccos Pending CN109964936A (en)

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CN114451165A (en) * 2022-02-24 2022-05-10 河南农业大学 Nitrogen synergist for regulating and controlling tobacco growth and use method and application thereof

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114451165A (en) * 2022-02-24 2022-05-10 河南农业大学 Nitrogen synergist for regulating and controlling tobacco growth and use method and application thereof
CN114451165B (en) * 2022-02-24 2022-12-13 河南农业大学 Nitrogen synergist for regulating and controlling tobacco growth and use method and application thereof

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Application publication date: 20190705