CN109959619B - Method for measuring accumulation rate of blue carbon in shellfish culture sediment - Google Patents

Method for measuring accumulation rate of blue carbon in shellfish culture sediment Download PDF

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CN109959619B
CN109959619B CN201910257386.7A CN201910257386A CN109959619B CN 109959619 B CN109959619 B CN 109959619B CN 201910257386 A CN201910257386 A CN 201910257386A CN 109959619 B CN109959619 B CN 109959619B
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范萍萍
吕美蓉
李雪莹
刘杰
刘岩
张颖颖
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Oceanographic Instrumentation Research Institute Shandong Academy of Sciences
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Abstract

The invention relates to the field of ecological environment monitoring and analysis, in particular to a method for detecting the accumulation rate of blue carbon in sediments in shellfish culture. A method for measuring the accumulation rate of blue carbon in shellfish culture comprises (1) setting m and n observation points in shellfish culture area and adjacent non-shellfish culture area respectively; (2) collecting sediment samples of an observation point, and constructing a spectrum model of carbon content in sediment; (3) measuring spectral data of the observation points; substituting the spectrum model to calculate the carbon content of the sediment section of the observation point; (4) converting the carbon content of the sediment section of the observation point into the carbon reserve of the observation point through a conversion coefficient t; (5) by the formula
Figure DDA0002014173630000011
And calculating the accumulation rate of the cultivated blue carbon in the sediment in a certain period of time. The method for measuring the accumulation rate of blue carbon in the sediment in shellfish culture solves the difficulty that the increase and decrease of discharge capacity of culture are disturbed, and is convenient for scientifically evaluating the contribution of culture carbon sink.

Description

Method for measuring accumulation rate of blue carbon in shellfish culture sediment
Technical Field
The invention relates to the field of ecological environment monitoring and analysis, in particular to a method for detecting the accumulation rate of blue carbon in sediments in shellfish culture.
Background
In the face of increasingly severe global climate change in carbon emission chain, biosolidation is a safe, efficient and economically viable sink-increasing approach. The mariculture can utilize a biological carbon sequestration strategy to the maximum extent, increases ocean carbon sink, and is a win-win way with sink increase and low carbon development explored in China in recent years.
China is the world with the largest mariculture scale, and the culture yield accounts for more than 60% of the world. Wherein, the shellfish and algae cultivation is taken as the main industry, and the yield of the shellfish and algae cultivation accounts for more than 85 percent of the marine cultivation yield of China. Shellfish and algae absorb CO2 in seawater through feeding activity and photosynthesis respectively to form huge biomass, and form removable blue carbon after being harvested. It is estimated that the blue carbon removed by the shellfish increases from 1400 to 1900 ten thousand tons per year with the blue carbon removed by the algae increasing from 1000 to 1200 ten thousand tons per year and the blue carbon removed by the algae increasing from 100 to 200 ten thousand tons per year during 2006-2016. Therefore, the cultivated blue carbon in China plays an important role in buffering global climate change.
However, apart from the biomass carbon sink that can be removed, the amount of invisible carbon sink that settles into the sediment is not negligible. However, due to the limitations of monitoring techniques, the amount of blue carbon in this deposit has not been quantified.
Disclosure of Invention
In order to make up for the defects in the prior art, the invention provides a method for measuring the accumulation rate of blue carbon in the shellfish culture sediment, and provides a necessary technical means for the research and analysis of the blue carbon culture.
The technical scheme adopted by the invention for solving the technical problems is as follows: a method for determining the accumulation rate of blue carbon in shellfish culture sediment, comprising the steps of:
(1) respectively arranging m observation points and n observation points in the shellfish culture area and the adjacent non-shellfish culture area, wherein m is more than or equal to 1, and n is more than or equal to 1;
(2) collecting sediment samples near the observation point, and constructing a spectrum model of the carbon content in the sediment;
(3) measuring spectral data of the observation points; substituting the spectrum model to calculate the carbon content of the sediment section of the observation point;
(4) converting the carbon content of the sediment section of the observation point into the carbon reserve of the observation point by a conversion coefficient t: t is the density of the deposit, the volume of the deposit at the observation point, the concentration conversion coefficient and the area of the deposit section corresponding to the carbon content;
(5) by the formula
Figure BDA0002014173610000021
Calculating the accumulation rate of the cultivated blue carbon in the sediment within a certain period of time; wherein: the delta Y is the difference value of the carbon reserves of the observation points of the culture area in a certain period of time delta t; and delta N is the difference value of the carbon reserves of the observation points of the non-culture area in a certain period of time delta t, delta t is a time period, and m and N are the numbers of the observation points of the corresponding culture area and the observation points of the non-culture area.
Further, the method for selecting observation points of the culture area and the non-culture area comprises the following steps: measuring various parameters of seawater and sediments at each observation point before shellfish cultivation and in non-cultivation areas in the cultivation areas, including pH, salinity, dissolved oxygen, nutritive salt, total organic carbon, biological oxygen demand of seawater, and depth, granularity, salinity, organic carbon, nitrogen and phosphorus content of sediments; if the parameters are not obviously different between the culture area and the non-culture area, setting the parameters as observation points; the non-culture area is close to the culture area, surrounds or semi-surrounds the culture area, and is about 100-200m away from the culture area.
Further, the method for constructing the spectrum model of the carbon content in the sediment comprises the following steps:
(1) collecting a sediment wet sample near an observation point, and measuring the visible-near infrared spectrum of the wet sample by a high-precision spectrometer;
(2) gradually drying the wet samples to form sediment samples with different moisture gradients, and then measuring the visible-near infrared spectrums of the sediment samples;
(3) a carbon content spectral model was established for the spectral data of the dried sediment samples:
Y=11.822X1+0.258X2+4.928X3+50.56
wherein: y is the carbon content of the deposit, X1-X3The reflectivities of the wavelengths corresponding to 440nm, 520nm and 620nm respectively;
(4) respectively carrying out high-order wavelet transformation on the sediment samples in the steps (1) and (2), eliminating the influence of moisture, extracting effective information of a carbon spectrum, and establishing a transfer relation between spectrum models of the carbon content of a wet sample and a dry sample: and y is ax + b.
The method for measuring the accumulation rate of the blue carbon in the shellfish culture sediment has the beneficial effects that: by deducting the carbon reserves in a certain period of time in the non-culture area, the invisible carbon sink generated by shellfish culture is well locked, the difficulty that the culture increases the sink and reduces the discharge capacity is solved, the test efficiency is greatly improved, and the contribution of the culture carbon sink is conveniently and rapidly evaluated scientifically.
Drawings
FIG. 1 is a visible-near infrared spectrum of a wet sample of a point-of-observation deposit;
FIG. 2 is a spectral model of carbon content in a dried sample;
FIG. 3 is a model transfer diagram of a high order wavelet analysis;
FIG. 4 shows the carbon content of the mactra veneriformis culture region at 9 months and 1 day;
FIG. 5 carbon content of 11 months and 30 days in each observation point of the bay clam culture area.
Detailed Description
The method for measuring the accumulation rate of blue carbon in the sediment of shellfish culture according to the invention is explained and illustrated in detail in the following with reference to the attached drawings and examples.
Example 1 the method of the present invention was used to detect the rate of accumulation of carbon sink in the sediment formed by mactra veneriformis breeding, and the specific steps were as follows:
firstly, selecting observation points: 1 observation point to be selected is respectively selected in a mactra breeding area (36 degrees 08 to 36 degrees 24 ' N,120 degrees 07 to 120 degrees 20 ' E) and an adjacent non-shellfish breeding area, the statistical difference of the parameters between the breeding area and the non-breeding area is calculated by measuring the parameters of the pH, the salinity, the dissolved oxygen, the nutritive salt, the total organic carbon, the biological oxygen demand, the depth, the granularity, the salinity, the organic carbon, the nitrogen, the phosphorus content and the like of the sediment of seawater, the observation points without significant difference are finally determined to be respectively marked as Y1(36 degrees 12 ' 21 ' N,120 degrees 16 ' E) and N1(36 degrees 12 ' 23 ' N,120 degrees 16 ' 18 ' E), and the observation points can represent the ecological environment before and after shellfish breeding in the breeding area.
Secondly, collecting sediment samples near the observation points, and constructing a spectrum model of the carbon content in the sediment
1. First, using wet samples of the deposit collected at the time of determination of the observation point, the visible-near infrared spectrum of these wet samples was measured by a high-precision spectrometer, as shown in fig. 1. However, because of the interference of moisture in the wet sample, a spectrum model of the carbon content needs to be established after the influence of the moisture in the wet sample is removed.
2. Gradually drying the wet sample through a moisture simulation experiment, respectively drying for 1h, 6h, 12h, 24h, 36h and 48h, and grinding to form sediment samples with different moisture gradients from 0-a%, wherein (a is the moisture content of fresh sediment samples without any drying treatment);
3. the carbon content (%) of the dried sediment sample with a water content of 0 was measured using an elemental analyzer, the visible-near infrared reflectance spectra of the sediment samples with different water gradients were measured using a spectrometer, and a spectrum model of the carbon content of the dried sediment was established by a spectral interpretation process, as shown in fig. 2:
Y=11.822X1+0.258X2+4.928X3+50.56
wherein: y is the carbon content of the deposit, X1-X3The reflectance is respectively corresponding to the wavelengths of 440nm, 520nm and 620 nm.
4. Respectively carrying out high-order wavelet transformation on the sediment samples in the steps 1 and 2, eliminating the influence of moisture, extracting effective information (high-order low-frequency part) of a carbon spectrum, and establishing a transfer relation between spectrum models of the carbon contents of a fresh wet sample and a dry sample: and performing correlation analysis on the low-frequency parts of the two samples to establish a transfer relationship of y being 0.81x +0.11, wherein x is the carbon content in the dry sample, and y is the carbon content in the corresponding wet sample.
The influence of moisture is eliminated by high-order wavelet analysis, as shown in fig. 3, fig. 3a is a low-frequency part (baseline) of wavelet analysis of a water-containing sample and a dry sample, which are basically overlapped, and fig. 3b is a model transmission process of the water-containing sample, namely, after moisture information of the water-containing sample is eliminated, a result (vertical axis) of prediction by a spectrum model of the dry sample is high in goodness of fit with an actually measured result (horizontal axis).
Thirdly, collecting samples of observation points respectively at 1 day of 9 months and 30 days of 11 months, measuring the spectrum data of each sample, and calculating the carbon content of each observation point in the gulf clam culture area at 1 day of 9 months and 30 days of 11 months by using the model transfer relationship, as shown in fig. 4 and 5.
Fourthly, converting the carbon content (%) of the sediment section at the observation point into the carbon reserve (g C/m) at the observation point by a conversion coefficient t2): t is deposit density (kg/m)3) X volume of deposit at observation point (m)3) X concentration conversion coefficient (g/kg)/area (m) of deposit cross section corresponding to the carbon content2) (ii) a Where t is 1g C/m2=1.5kg/m3×5.0×10-5m3×10g/kg/(0.75×10-4m2)。
Fifthly, according to the measured carbon reserves, the formula is adopted
Figure BDA0002014173610000061
The carbon accumulation rate in autumn in the gulf clam culture area is calculated and shown in table 1.
TABLE 1 autumn carbon accumulation rate in Bay clam culture area
Figure BDA0002014173610000062
The foregoing is considered as illustrative only of the principles of the invention and is not to be taken in any way limiting, since all equivalent changes and modifications are intended to be included within the scope of the appended claims.

Claims (3)

1. A method for determining the accumulation rate of blue carbon in shellfish culture sediment, comprising the steps of:
(1) respectively arranging m observation points and n observation points in the shellfish culture area and the adjacent non-shellfish culture area, wherein m is more than or equal to 1, and n is more than or equal to 1;
(2) collecting sediment samples near the observation point, and constructing a spectrum model of the carbon content in the sediment;
(3) measuring spectral data of the observation points; substituting the spectrum model to calculate the carbon content of the sediment section of the observation point;
(4) converting the carbon content of the sediment section of the observation point into the carbon reserve of the observation point by a conversion coefficient t: t is the deposit density x the volume of the deposit x the concentration conversion factor/area of the deposit cross section corresponding to the carbon content;
(5) by the formula
Figure FDA0003068066950000011
Calculating the accumulation rate of the cultivated blue carbon in the sediment within a certain period of time; wherein: the delta Y is the difference value of the carbon reserves of the observation points of the culture area in a certain period of time delta t; delta N is the difference value of carbon reserves of observation points of a non-cultivation area within a certain period of time delta t, delta t is a time period, and m and N are the number of observation points of the corresponding cultivation area and observation points of the non-cultivation area;
the method for constructing the spectrum model of the carbon content in the sediment comprises the following steps:
(a) collecting a sediment wet sample near an observation point, and measuring the visible-near infrared spectrum of the wet sample by a high-precision spectrometer;
(b) gradually drying the wet samples to form sediment samples with different moisture gradients, and then measuring the visible-near infrared spectrums of the sediment samples;
(c) a carbon content spectral model was established for the spectral data of the dried sediment samples:
Y=11.822X1+0.258X2+4.928X3+50.56
wherein: y is the carbon content of the deposit, and X1-X3 are respectively the reflectivities of the corresponding wavelengths of 440nm, 520nm and 620 nm;
(d) respectively carrying out high-order wavelet transformation on the sediment samples in the step (a) and the step (b), eliminating the influence of moisture, extracting effective information of a carbon spectrum, and establishing a transfer relation between spectrum models of the carbon content of a wet sample and a dry sample: y is ax + b; where a is the slope of the line and b is the intercept.
2. The method for determining the accumulation rate of blue carbon in the sediments in shellfish farming according to claim 1, wherein the observation points of the farming area and the non-farming area are selected by the following method: measuring various parameters of seawater and sediments at each observation point of the culture area and the non-culture area before shellfish culture in the culture area, and setting the parameters as the observation points if the measured parameters have no significant difference between the culture area and the non-culture area; the non-culture area is close to the culture area and surrounds or semi-surrounds the culture area, and the distance between the non-culture area and the culture area is 100-200 m.
3. The method for determining the accumulation rate of blue carbon in shellfish culture according to claim 2, wherein said parameters comprise: the pH, salinity, dissolved oxygen, nutrient salts, total organic carbon and biological oxygen demand of the seawater, and the depth, particle size, salinity, organic carbon, nitrogen and phosphorus content of the sediment.
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