CN109957494A - A kind of temprature control method for nucleic acid augmentative instrument - Google Patents
A kind of temprature control method for nucleic acid augmentative instrument Download PDFInfo
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- CN109957494A CN109957494A CN201910219411.2A CN201910219411A CN109957494A CN 109957494 A CN109957494 A CN 109957494A CN 201910219411 A CN201910219411 A CN 201910219411A CN 109957494 A CN109957494 A CN 109957494A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q3/00—Condition responsive control processes
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- G—PHYSICS
- G05—CONTROLLING; REGULATING
- G05D—SYSTEMS FOR CONTROLLING OR REGULATING NON-ELECTRIC VARIABLES
- G05D23/00—Control of temperature
- G05D23/19—Control of temperature characterised by the use of electric means
- G05D23/20—Control of temperature characterised by the use of electric means with sensing elements having variation of electric or magnetic properties with change of temperature
Abstract
A kind of temprature control method for nucleic acid augmentative instrument provided by the invention includes input unit, temperature sensor group, chip, level-one cooling piece, second level cooling piece, radiating module, PID control unit；The temperature sensor group includes the first temperature sensor, second temperature sensor, third temperature sensor, the 4th temperature sensor；The PID control unit includes processes temperature signal unit, fuzzy operation device, PID controller, and the fuzzy operation device is electrically connected with the PID controller；The processes temperature signal unit is electrically connected with the PID controller；The PID control unit makes a policy according to the result of fuzzy operation device and PID controller, control level-one cooling piece, second level cooling piece, radiating module work；Make temperature adjustment rapid reaction provided by the present invention for the temprature control method of nucleic acid augmentative instrument and accuracy is high.
Description
Technical field
The present invention relates to nucleic acid augmentative instrument technical fields, and in particular to a kind of temperature controlling party for nucleic acid augmentative instrument
Method.
Background technique
Nucleic acid amplification technologies, (Polymerase Chain Reaction is referred to as especially digital polymerase chain reaction technique
It PCR), is a kind of for expanding the molecular biotechnology of specific DNA molecular segment, it is using DNA molecular as template, by a pair of of people
The specific oligonucleotide primers of work synthesis, pass through archaeal dna polymerase enzymatic reaction, rapid amplifying specific DNA molecular segment, in biology
There is extremely important effect on.The basic process of PCR reaction is divided into three steps.The first step, DNA are denaturalized (94 DEG C), double-stranded DNA
Template hydrogen bond under heat effect is broken, and forms single stranded DNA；Second step is annealed (55 DEG C), and system temperature reduces, primer and DNA mould
Hardened conjunction forms local double-strand；Third step extends (72 DEG C), is raw material from 5 ends of primer using dNTP under the action of Taq enzyme
Extend to 3 ends, synthesizes the DNA chain complementary with template.PCR instrument is exactly to pass through control sample to reach different temperatures, to what is be amplified
DNA fragmentation is denaturalized, is annealed and polymerization processing, to achieve the purpose that at double to expand the amount of DNA fragmentation.Therefore, temperature control
The precision of system, the speed of heating and cooling directly affect the efficiency of DNA fragmentation amplification.
Chinese patent (CN105573368A) discloses a kind of temprature control method of PCR instrument, comprising the following steps: step
One, the temperature of PCR instrument each section is detected respectively using three temperature sensors；Step 2, processes temperature signal；Step
Three, PC machine calculates proportional control factor, integration control system with genetic algorithm according to the change rate of temperature deviation value and deviation
Several and derivative control coefficient, and temperature control command is issued to main control chip；Step 4, control command receive, and temperature is adjusted in real time
Section；Step 5, temperature control curve real-time display.Although the patent step is simple, has equally used PID adjustment parameter,
This method does not provide the specific algorithm of PID controller, although while adjustable temperature, energy consumption it is larger.
Chinese patent (CN108192997A) discloses the temprature control method and device of a kind of PCR instrument orifice plate subregion,
In, method includes: to obtain the set temperature and Current Temperatures of each subregion of orifice plate, wherein set temperature is corresponding orifice plate subregion
To be controlled the temperature reached；The first heating power of each subregion of orifice plate is obtained according to set temperature and Current Temperatures；According to hole
The heating module of the first heating power control each subregion of orifice plate of each subregion of plate is heated.By according to set temperature and currently
Temperature obtains the first heating power of each subregion of orifice plate, controls each subregion of orifice plate using corresponding first heating power of each subregion
Heating module corresponding region is heated, which enables to different subregions while reaching set temperature, thus
It ensure that temperature controlled synchronism, but the invention temperature control precision is not high, and can not achieve the skill of fast cooling heating
Art effect.
Summary of the invention
In view of the above-mentioned deficiencies in the prior art, the technical problem to be solved by the present invention is that providing a kind of for nucleic acid
The temprature control method of amplification instrument.
The present invention is that technical solution used by solving its technical problem is: on the one hand, being provided a kind of for nucleic acid amplification
The temprature control method of instrument, includes the following steps
S1: preset temperature T0, the first temperature sensor, second temperature sensor, third temperature are inputted in input unit
Sensor, the 4th temperature sensor carry out temperature signal to chip, level-one cooling piece, second level cooling piece, radiating module respectively and adopt
Collection, respectively obtains the first signal temperature, second signal temperature, third signal temperature, fourth signal temperature；
S2: by the first temperature signal collected in step S1, second temperature signal, third temperature signal, the 4th temperature
Signal is separately converted to be transferred to PID controller after digital quantity T1, T2, T3, T4 and be handled by processes temperature signal unit；
S3: obtaining preset temperature T0, obtains the chip temperature T1 of the first temperature sensor acquisition, obtains second, third temperature
The level-one cooling piece of sensor acquisition, temperature T2, T3 of second level cooling piece are spent, the radiating mould of the 4th temperature sensor acquisition is obtained
The temperature T4 of block；
S4: temperature T2, T3 of the first cooling piece and the second cooling piece is averaged；Obtain T5；
S5: temperature error e=T1-T0, temperature error change rate ec=de/dt are calculated；
S6: fuzzy operation device is calculated output and is become using temperature error e, temperature error change rate ec as Indistinct Input
Measure proportionality coefficient correction value Kp, integral coefficient correction value Ki and differential coefficient correction value Kd；
S7: final ratio corrected parameter Kp '=Kp* (T1-T5) (T4-T1), final integral corrected parameter Ki ' are calculated
=Ki* (T1-T5) (T4-T1), final differential corrected parameter Kd '=Kd* (T1-T5) (T4-T1)；
S8:PID controller is with proportionality coefficient end value Kp ', integral coefficient end value Ki ' and differential coefficient end value
Kd ' is foundation, controls the execution of level-one cooling piece, second level cooling piece and radiating module.
It preferably, further include that ratio output U is calculated separately according to Kp ', Ki ', Kd ' in the step S8p=Kp ' * e；Product
Divide output Ui=Ui-1+Ki′*(e-ec)；Differential exports Ud=Ud-1+ Kd ' * (e-ea), in formula, ea is previous error；Ud-1
For the output of previous differential, Ui-1For the output of previous integral；It calculates PID controller and exports output=Up+Ui+Ud+ M, M
To correct constant.
It preferably, further include the size relation that output and 0 is judged by judging unit in the step S8, if output
> 0, then control level-one cooling piece, second level cooling piece continues to heat, and control the output of adjustment level-one cooling piece, second level cooling piece
Power；If output < 0, control radiating module and continue to radiate, and controls the output power of adjustment radiating module.
Preferably, control adjusts level-one cooling piece, the output power of second level cooling piece includes that the first temperature that will acquire passes
The chip temperature T1 of sensor acquisition is compared with preset temperature value T0, if T0-T1 > 0, increases level-one cooling piece, two-stage system
Cold output power.
Preferably, set heating decision content as Δ T1, if T0-T1 > Δ T1, by level-one cooling piece and second level cooling piece with
100% power mode works；If 0 < T0-T1 < Δ T1, then by the output power of level-one cooling piece and second level cooling piece with α
Power works, α=T0-T1/ Δ T1.
Preferably, the output power of control adjustment radiating module includes that the first temperature sensor that will acquire that will acquire is adopted
The chip temperature T1 of collection is compared with preset temperature value T0, if T1-T0 > 0, increases the output power of radiating module.
Preferably, set refrigeration decision content as Δ T2, if T1-T0 > Δ T2, by the output power of radiating module with
100% power mode carries out, if 0 < T1-T0 < Δ T2, by the output power of radiating module with the progress of β power mode, β=T1-
ΔT2/T0。
On the other hand, a kind of temperature control system for nucleic acid augmentative instrument, including input unit, temperature sensor are provided
Group, chip, level-one cooling piece, second level cooling piece, radiating module, PID control unit；The temperature sensor group includes the first temperature
Spend sensor, second temperature sensor, third temperature sensor, the 4th temperature sensor；The PID control unit includes temperature
Signal processing unit, fuzzy operation device, PID controller, the fuzzy operation device are electrically connected with the PID controller；The temperature
Degree signal processing unit is electrically connected with the PID controller；The PID control unit is according to fuzzy operation device and PID controller
Result make a policy, control level-one cooling piece, second level cooling piece, radiating module work.
Preferably, the processes temperature signal unit includes temperature signal amplifying circuit, temperature signal filter circuit, A/D
Conversion circuit and temperature signal protect circuit；The temperature signal amplifying circuit, temperature signal filter circuit, A/D conversion circuit
It is successively connect with the PID controller with temperature signal protection circuit；The temperature signal filter circuit includes parallel with one another
Low frequency filtering subelement, High frequency filter subelement.
It compares with the prior art, the beneficial effect comprise that:
(1) fuzzy operation algorithm is added in the present invention in traditional PID temperature control algolithm, by temperature difference and temperature
It spends poor change rate progress fuzzy operation and obtains corresponding proportionality coefficient, integral coefficient and differential coefficient, and according to the reality of invention
Border situation further corrects proportionality coefficient of the invention, integral coefficient and differential coefficient, while according to ratio output, integral
Output and differential output make decisions to level-one cooling piece, second level cooling piece and radiating module, and it is quasi- to greatly improve temperature control
Exactness improves temperature-controlled precision.
(2) provided by the present invention for the PID control unit of the temperature control system offer of nucleic acid augmentative instrument according to fuzzy
The result of arithmetic unit and PID controller makes a policy, control level-one cooling piece, second level cooling piece, radiating module work.
(3) difference of the invention also according to preset temperature value and real-time chip temperature, to level-one cooling piece, second level cooling piece
Real-time adjustment is made with the power of radiating module, reduces energy consumption to the greatest extent, while the present invention improves control system
Performance realizes energy-saving and emission-reduction.
(4) pid parameter method of adjustment provided by the invention, calculation method is simple, and can scientific and simple adjusting level-one system
Cold, the output power of second level cooling piece and radiating module, can be achieved with to the good of the temperature control system of nucleic acid augmentative instrument
It adjusts.
(5) present invention uses fuzzy-adaptation PID control, and apparatus structure is simple, and parameter regulation stability is good, can accurately control
Temperature.
Detailed description of the invention
Fig. 1 is nucleic acid augmentative instrument control method flow chart provided by the invention；
Specific embodiment
With reference to the accompanying drawing, a specific embodiment of the invention is described in detail.
The present invention devises a kind of temperature control system of automation on the basis of conventional temperature control method, including
Input unit, temperature sensor group, chip, level-one cooling piece, second level cooling piece, radiating module, PID control unit；The temperature
Spending sensor group includes the first temperature sensor, second temperature sensor, third temperature sensor, the 4th temperature sensor；Institute
Stating PID control unit includes processes temperature signal unit, fuzzy operation device, PID controller, the fuzzy operation device with it is described
PID controller electrical connection；Processes temperature signal unit is electrically connected with the PID controller；The PID control unit is according to fuzzy
The result of arithmetic unit and PID controller makes a policy, control level-one cooling piece, second level cooling piece, radiating module work.
Wherein processes temperature signal unit provided by the invention includes temperature signal amplifying circuit, temperature signal filtered electrical
Road, A/D conversion circuit and temperature signal protect circuit；The temperature signal amplifying circuit, temperature signal filter circuit, A/D turn
It changes circuit and temperature signal protection circuit is successively connect with the PID controller；The temperature signal filter circuit includes mutual
Low frequency filtering subelement, the High frequency filter subelement of parallel connection.
Wherein, the first temperature sensor, second temperature sensor, the third temperature sensor, provided in the present embodiment
Four temperature sensors are a kind of high-sensitivity digital formula temperature sensor；
The radiating module provided in the present embodiment uses fan.
As shown in Figure 1, the present invention also provides a kind of temprature control methods for nucleic acid augmentative instrument, including walk as follows
It is rapid:
S1: preset temperature T0, the first temperature sensor, second temperature sensor, third temperature are inputted in input unit
Sensor, the 4th temperature sensor carry out temperature signal to chip, level-one cooling piece, second level cooling piece, radiating module respectively and adopt
Collection, respectively obtains the first signal temperature, second signal temperature, third signal temperature, fourth signal temperature；
S2: by the first temperature signal collected in step S1, second temperature signal, third temperature signal, the 4th temperature
Signal is separately converted to be transferred to PID controller after digital quantity T1, T2, T3, T4 and be handled by processes temperature signal unit；
S3: obtaining preset temperature T0, obtains the chip temperature T1 of the first temperature sensor acquisition, obtains second, third temperature
The level-one cooling piece of sensor acquisition, temperature T2, T3 of second level cooling piece are spent, the radiating mould of the 4th temperature sensor acquisition is obtained
The temperature T4 of block；
S4: temperature T2, T3 of the first cooling piece and the second cooling piece is averaged；Obtain T5；
S5: temperature error e=T1-T0, temperature error change rate ec=de/dt are calculated；
S6: fuzzy operation device is calculated output and is become using temperature error e, temperature error change rate ec as Indistinct Input
Measure proportionality coefficient correction value Kp, integral coefficient correction value Ki and differential coefficient correction value Kd；
S7: final ratio corrected parameter Kp '=Kp* (T1-T5) (T4-T1), final integral corrected parameter Ki ' are calculated
=Ki* (T1-T5) (T4-T1), final differential corrected parameter Kd '=Kd* (T1-T5) (T4-T1)；
S8:PID controller is with proportionality coefficient end value Kp ', integral coefficient end value Ki ' and differential coefficient end value
Kd ' is foundation, controls the execution of level-one cooling piece, second level cooling piece and radiating module.
It wherein, further include that ratio output U is calculated separately according to Kp ', Ki ', Kd ' in step S8p=Kp ' * e；Integral output Ui
=Ui-1+Ki′*(e-ec)；Differential exports Ud=Ud-1+ Kd ' * (e-ea), in formula, ea is previous error；Ud-1It is preceding primary
Differential output, Ui-1For the output of previous integral；It calculates PID controller and exports output=Up+Ui+Ud+ M, M are that amendment is normal
Number.
It further include that the size relation that output and 0 is judged by judging unit if output > 0 controls one in step S8
Grade cooling piece, second level cooling piece continue to heat, and control the output power of adjustment level-one cooling piece, second level cooling piece；If
Output < 0 then controls radiating module and continues to radiate, and controls the output power of adjustment radiating module.
Control adjusts level-one cooling piece, the output power of second level cooling piece includes the first temperature sensor acquisition that will acquire
Chip temperature T1 be compared with preset temperature value T0, if T0-T1 > 0, increase level-one cooling piece, second level cooling piece it is defeated
Power out.
Heating decision content is set as Δ T1, if T0-T1 > Δ T1, by level-one cooling piece and second level cooling piece with 100% function
Rate mode works；If 0 < T0-T1 < Δ T1, then by the output power of level-one cooling piece and second level cooling piece with the progress of α power
Work, α=T0-T1/ Δ T1.
The output power of control adjustment radiating module includes the core for the first temperature sensor acquisition that will acquire that will acquire
Piece temperature T1 is compared with preset temperature value T0, if T1-T0 > 0, increases the output power of radiating module.
Refrigeration decision content is set as Δ T2, if T1-T0 > Δ T2, by the output power of radiating module with 100% power mould
Formula carries out, if 0 < T1-T0 < Δ T2, by the output power of radiating module with the progress of β power mode, β=T1- Δ T2/T0.
Fuzzy operation algorithm is added in the present invention in traditional PID temperature control algolithm, by temperature difference and temperature
Poor change rate carries out fuzzy operation and obtains corresponding proportionality coefficient, integral coefficient and differential coefficient, and according to the reality of invention
Situation further corrects proportionality coefficient of the invention, integral coefficient and differential coefficient, at the same according to ratio output, integrate it is defeated
Out and differential output makes decisions to level-one cooling piece, second level cooling piece and radiating module, greatly improves temperature precise control
Degree improves temperature-controlled precision.Pid parameter method of adjustment provided by the invention simultaneously, parameter regulation stability is good, calculation method letter
It is single, and the output power of the adjusting level-one cooling piece of energy science, second level cooling piece and radiating module, it can be achieved with to nucleic acid amplification
The well-tuned of the temperature control system of instrument.Energy consumption can also be reduced to the greatest extent.
The preferred embodiment for the present invention is explained in detail above in conjunction with attached drawing, but the present invention is not limited to above-mentioned implementations
Mode within the knowledge of a person skilled in the art can also be without departing from the purpose of the present invention
It makes a variety of changes.
Many other changes and remodeling can be made by not departing from the spirit and scope of the present invention.It should be appreciated that the present invention is not
It is limited to specific embodiment, the scope of the present invention is defined by the following claims.
Claims (9)
1. a kind of temprature control method for nucleic acid augmentative instrument, which comprises the following steps:
S1: preset temperature T0, the first temperature sensor, second temperature sensor, third temperature sensing are inputted in input unit
Device, the 4th temperature sensor carry out temperature signal collection to chip, level-one cooling piece, second level cooling piece, radiating module respectively, point
The first signal temperature, second signal temperature, third signal temperature, fourth signal temperature are not obtained；
S2: by the first temperature signal collected in step S1, second temperature signal, third temperature signal, the 4th temperature signal
It is separately converted to be transferred to PID controller after digital quantity T1, T2, T3, T4 by processes temperature signal unit and be handled；
S3: obtaining preset temperature T0, obtains the chip temperature T1 of the first temperature sensor acquisition, obtains second, third temperature biography
Temperature T2, T3 of level-one cooling piece, second level cooling piece that sensor acquires obtains the radiating module of the 4th temperature sensor acquisition
Temperature T4；
S4: temperature T2, T3 of the first cooling piece and the second cooling piece is averaged；Obtain T5；
S5: temperature error e=T1-T0, temperature error change rate ec=de/dt are calculated；
S6: output variable ratio is calculated using temperature error e, temperature error change rate ec as Indistinct Input in fuzzy operation device
Example coefficient correction value Kp, integral coefficient correction value Ki and differential coefficient correction value Kd；
S7: calculating final ratio corrected parameter Kp '=Kp* (T1-T5) (T4-T1), and final integral corrected parameter Ki '=
Ki* (T1-T5) (T4-T1), final differential corrected parameter Kd '=Kd* (T1-T5) (T4-T1)；
S8:PID controller is with proportionality coefficient end value Kp ', integral coefficient end value Ki ' and differential coefficient end value Kd '
Foundation controls the execution of level-one cooling piece, second level cooling piece and radiating module.
2. being used for the temprature control method of nucleic acid augmentative instrument as described in claim 1, which is characterized in that in the step S8 also
Including calculating separately ratio output Up=Kp ' * e according to Kp ', Ki ', KD '；Integral output Ui=Ui-1+Ki′*(e-ec)；Differential
Export Ud=Ud-1+ Kd ' * (e-ea), in formula, ea is previous error；It calculates PID controller and exports output=Up+Ui+Ud
+ M, M are amendment constant.
3. being used for the temprature control method of nucleic acid augmentative instrument as claimed in claim 2, which is characterized in that in the step S8 also
Size relation including judging output and 0 by judging unit controls level-one cooling piece, second level refrigeration if output > 0
Piece continues to heat, and controls the output power of adjustment level-one cooling piece, second level cooling piece；If output < 0 controls radiating mould
Block continues to radiate, and controls the output power of adjustment radiating module.
4. being used for the temprature control method of nucleic acid augmentative instrument as claimed in claim 3, which is characterized in that control adjustment level-one system
Cold, the output power of second level cooling piece include the first temperature sensor acquisition that will acquire chip temperature T1 and preset temperature
Value T0 is compared, if T0-T1 > 0, increases the output power of level-one cooling piece, second level cooling piece.
5. being used for the temprature control method of nucleic acid augmentative instrument as claimed in claim 4, which is characterized in that setting heating decision content
Level-one cooling piece is worked with second level cooling piece with 100% power mode if T0-T1 > Δ T1 for Δ T1；If 0 < T0-
T1 < Δ T1 is then worked level-one cooling piece and the output power of second level cooling piece with α power, α=T0-T1/ Δ T1.
6. being used for the temprature control method of nucleic acid augmentative instrument as claimed in claim 3, which is characterized in that control adjustment radiating mould
The output power of block includes the chip temperature T1 and preset temperature value T0 for the first temperature sensor acquisition that will acquire that will acquire
It is compared, if T1-T0 > 0, increases the output power of radiating module.
7. being used for the temprature control method of nucleic acid augmentative instrument as claimed in claim 6, which is characterized in that setting refrigeration decision content
The output power of radiating module is carried out with 100% power mode if T1-T0>Δ T2 for Δ T2, if 0<T1-T0<Δ T2,
Then by the output power of radiating module with the progress of β power mode, β=T1- Δ T2/T0.
8. special such as the control system of the temprature control method of any of claims 1-7 for nucleic acid augmentative instrument
Sign is, including input unit, temperature sensor group, chip, level-one cooling piece, second level cooling piece, radiating module, PID control
Unit；The temperature sensor group includes the first temperature sensor, second temperature sensor, third temperature sensor, the 4th temperature
Spend sensor；The PID control unit includes processes temperature signal unit, fuzzy operation device, PID controller, the fuzzy fortune
Device is calculated to be electrically connected with the PID controller；The processes temperature signal unit is electrically connected with the PID controller；The PID control
Unit processed makes a policy according to the result of fuzzy operation device and PID controller, control level-one cooling piece, second level cooling piece, heat dissipation
Module work.
9. being used for the temprature control method of nucleic acid augmentative instrument as claimed in claim 8, which is characterized in that at the temperature signal
Reason unit includes temperature signal amplifying circuit, temperature signal filter circuit, A/D conversion circuit and temperature signal protection circuit；Institute
State temperature signal amplifying circuit, temperature signal filter circuit, A/D conversion circuit and temperature signal protection circuit successively with it is described
PID controller connection；The temperature signal filter circuit includes low frequency filtering subelement parallel with one another, High frequency filter list
Member.
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| CN114812005A (en) * | 2022-06-28 | 2022-07-29 | 合肥中科国腾生物科技有限公司 | Active rapid heat dissipation method and device of thermal cycler based on thermoelectric refrigeration piece |
| CN115197835A (en) * | 2022-09-15 | 2022-10-18 | 浙江正合谷生物科技有限公司 | Nucleic acid amplification fluorescence quantitative temperature control system based on self-learning |
| US11938485B2 (en) | 2021-12-07 | 2024-03-26 | Industrial Technology Research Institute | Heating device for convective polymerase chain reaction |
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| CN110684643B (en) * | 2019-10-30 | 2021-03-19 | 宁波胤瑞生物医学仪器有限责任公司 | Temperature conduction system for nucleic acid amplification instrument |
| CN113377140A (en) * | 2021-06-09 | 2021-09-10 | 厦门大学 | Temperature control method and device for nucleic acid detection device |
| CN113377140B (en) * | 2021-06-09 | 2022-10-04 | 厦门大学 | Temperature control method and device for nucleic acid detection device |
| CN113485478A (en) * | 2021-07-07 | 2021-10-08 | 维柯基科技(上海)有限公司 | Temperature control method, device and system of PCR amplification instrument, computer equipment and computer readable storage medium |
| US11938485B2 (en) | 2021-12-07 | 2024-03-26 | Industrial Technology Research Institute | Heating device for convective polymerase chain reaction |
| CN114812005A (en) * | 2022-06-28 | 2022-07-29 | 合肥中科国腾生物科技有限公司 | Active rapid heat dissipation method and device of thermal cycler based on thermoelectric refrigeration piece |
| CN114812005B (en) * | 2022-06-28 | 2022-10-11 | 合肥中科国腾生物科技有限公司 | Active rapid heat dissipation method and device of thermal cycler based on thermoelectric refrigeration piece |
| CN115197835A (en) * | 2022-09-15 | 2022-10-18 | 浙江正合谷生物科技有限公司 | Nucleic acid amplification fluorescence quantitative temperature control system based on self-learning |
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