CN109976412A - A kind of temprature control method and device of nucleic acid augmentative instrument - Google Patents
A kind of temprature control method and device of nucleic acid augmentative instrument Download PDFInfo
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- CN109976412A CN109976412A CN201910219767.6A CN201910219767A CN109976412A CN 109976412 A CN109976412 A CN 109976412A CN 201910219767 A CN201910219767 A CN 201910219767A CN 109976412 A CN109976412 A CN 109976412A
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
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- G05D—SYSTEMS FOR CONTROLLING OR REGULATING NON-ELECTRIC VARIABLES
- G05D23/00—Control of temperature
- G05D23/19—Control of temperature characterised by the use of electric means
- G05D23/30—Automatic controllers with an auxiliary heating device affecting the sensing element, e.g. for anticipating change of temperature
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Abstract
The present invention provides a kind of temprature control methods for digitizing nucleic acid augmentative instrument.It obtains the Current Temperatures and set temperature of chip the following steps are included: step 1；Step 2, the duration for presetting and heating or freezing to chip is obtained；Step 3, according to pre-set duration control cooling piece adjusting chip temperature to set temperature, and the heating for controlling cooling piece or cooling time are less than or equal to preset value.Temprature control method provided by the invention, while guaranteeing rapid temperature rise and drop, energy consumption is reduced as far as possible by two-stage cooling piece mating reaction, improve the working efficiency of cooling piece, the relationship between temperature regulating time and power is balanced, quickly the temperature of chip can be adjusted, ensure that the efficient progress of PCR reaction, efficiently solve that existing temperature control system precision is not high, the problems such as the inefficiency of DNA fragmentation amplification.
Description
Technical field
The invention belongs to nucleic acid augmentative instrument technical field more particularly to a kind of temprature control method of nucleic acid augmentative instrument and
Device.
Background technique
Nucleic acid amplification technologies namely polymerase chain reaction technology (Polymerase Chain Reaction abbreviation PCR),
It is a kind of for expanding the molecular biotechnology of specific DNA molecular segment, it is using DNA molecular as template, by a pair of artificial conjunction
At specific oligonucleotide primers, pass through archaeal dna polymerase enzymatic reaction, rapid amplifying specific DNA molecular segment, biologically
With extremely important effect.The basic process of PCR reaction is divided into three steps.The first step, DNA are denaturalized (94 DEG C), double-stranded DNA template
Hydrogen bond is broken under heat effect, forms single stranded DNA；Second step is annealed (55 DEG C), and system temperature reduces, primer and DNA profiling knot
It closes, forms local double-strand；Third step extends (72 DEG C), under the action of Taq enzyme, using dNTP be raw material from the 5 ' of primer hold to
3 ' ends extend, and synthesize the DNA chain complementary with template.PCR instrument is exactly to pass through control sample to reach different temperatures, to what is be amplified
DNA fragmentation is denaturalized, is annealed and polymerization processing, to achieve the purpose that at double to expand the amount of DNA fragmentation.Therefore, temperature control
The precision of system, the speed of heating and cooling directly affect the efficiency of DNA fragmentation amplification.
The precision that existing nucleic acid amplification temprature control method is adjusted is lower, the bad control of the power of cooling piece, and chip
Temperature acquisition it is inaccurate, affect DNA fragmentation amplification efficiency.
Summary of the invention
The present invention is directed to problem of the prior art, proposes a kind of temprature control method of fast, accurately nucleic acid augmentative instrument
And device reduces energy consumption by two-stage cooling piece mating reaction while guaranteeing rapid temperature rise and drop as far as possible, improves
The working efficiency of cooling piece balances the relationship between the time and efficiency of temperature adjusting, can quickly to the temperature of chip
It is adjusted, ensure that the efficient progress of PCR reaction, it is not high to efficiently solve existing temperature control system precision, and DNA fragmentation expands
The problems such as inefficiency of increasing.
Specifically, the present invention is implemented using following technical scheme:
A kind of temprature control method of nucleic acid augmentative instrument, the nucleic acid augmentative instrument include the chip for holding sample, for pair
The cooling piece of chip heating and refrigeration for treatment temperature signal and issues temperature for obtaining the temperature sensor of temperature signal
Spend the master control borad of control command, the temprature control method of the nucleic acid augmentative instrument the following steps are included:
Step 1, the Current Temperatures T and set temperature T of the chip are obtained0；
Step 2, the duration for presetting and heating or freezing to the chip is obtained, preset value t is obtained0；
Step 3, control cooling piece adjusts chip temperature to set temperature, and controls heating or the cooling time t of cooling piecen
Less than or equal to preset value t0；
Further, the concrete mode of chip temperature to set temperature is adjusted in the step 3 are as follows: the processing mould of master control borad
Block obtains output valve U by formula A, and output valve U is converted to the temperature adjustment signal of analog current, then exports temperature adjustment signal again
To cooling piece, cooling piece carries out the adjustment of power,
Formula A:U=Ka|T-T0|+Kb d|T-T0|/dt+Kc∫|T-T0| dt, wherein U is output valve, and T is the current of chip
Temperature, T0For the set temperature of chip, KaFor proportionality coefficient, KbFor derivative time constant, KcFor integration time constant；
Further, the cooling piece includes level-one cooling piece and second level cooling piece；
Further, cooling piece adjusts the power of cooling piece according to formula B,
Formula B:P1 or P2=CM | T-T0|/(2tnK), wherein P1 and P2 is respectively level-one cooling piece and second level cooling piece
Realtime power, C are the specific heat capacity of chip, and M is the quality of chip, and T is the Current Temperatures of chip, T0For the set temperature of chip, tn
Heating or cooling time for cooling piece, K are the heat conduction efficiency of level-one cooling piece or second level cooling piece；
Further, preset value t in the step 20For 5-30s；
Further, the level-one cooling piece and second level cooling piece are separately provided for adjusting the adjusting of voltage or electric current
Device, the regulating device and master control borad are electrically connected, and the regulating device can be used for the adjusting of voltage or electric current to reach
Required power；
Further, the present invention can pass through comparison preset temperature difference and chip Current Temperatures T with preset temperature difference
With chip set temperature T0Temperature absolute value of the difference (i.e. | T-T0|) size select level-one cooling piece and second level cooling piece
Open state；
Further, if the temperature absolute value of the difference is less than or equal to the preset temperature difference, level-one refrigeration is opened
Piece carries out temperature adjusting；If the temperature absolute value of the difference is greater than the preset temperature difference, level-one cooling piece is opened simultaneously
Temperature adjusting is carried out with second level cooling piece；
Further, the preset temperature difference can be 5-20 DEG C；
Further, the Current Temperatures T of the chip is that the first temperature sensor and second temperature sensor collect
Chip temperature collection value T1 and T2 mean value；
Further, the nucleic acid augmentative instrument further includes the chip slot for holding chip, first temperature sensor and
Two temperature sensors are arranged between the center and peripheral of chip slot, and equidistantly distributed；
The present invention also protects a kind of temperature control equipment of nucleic acid augmentative instrument, and the nucleic acid augmentative instrument includes holding sample
Chip, the cooling piece for heating and freezing to chip are believed for obtaining the temperature sensor of temperature signal for treatment temperature
Number and issue the master control borad of temperature control command, the temperature control equipment of the nucleic acid augmentative instrument includes:
First acquisition unit, for obtaining the Current Temperatures T and set temperature T of the chip0；
Second acquisition unit obtains preset value t for obtaining the duration for presetting and heating or freezing to the chip0；
Control unit adjusts chip temperature to set temperature for controlling cooling piece, and when the heating or refrigeration of cooling piece
Between tn be less than or equal to preset value t0；
Further, described control unit includes:
First computing module, for obtaining output valve U by formula A,
Formula A:U=Ka|T-T0|+Kbd|T-T0|/dt+Kc∫|T-T0| dt, wherein U is output valve, and T is the current of chip
Temperature, T0For the set temperature of chip, KaFor proportionality coefficient, KbFor derivative time constant, KcFor integration time constant；
Then first processing module exports temperature adjustment signal for output valve U to be converted to the temperature adjustment signal of analog current again
To cooling piece；
Further, the cooling piece includes level-one cooling piece and second level cooling piece, described control unit further include:
Second computing module, for obtaining the power of cooling piece by formula B,
Formula B:P1 or P2=CM | T-T0|/(2tnK), wherein P1 and P2 is respectively level-one cooling piece and second level cooling piece
Realtime power, C are the specific heat capacity of chip, and M is the quality of chip, and T is the Current Temperatures of chip, T0For the set temperature of chip, tn
Heating or cooling time for cooling piece, K are the heat conduction efficiency of level-one cooling piece or second level cooling piece；
Second processing module, the power for being calculated according to the second computing module carry out the realtime power of cooling piece
It adjusts；
Further, the nucleic acid augmentative instrument further includes LCD display, and the temperature that master control borad can be obtained according to detection is believed
Number and nucleic acid augmentative instrument operation conditions, temperature control curve is obtained by the graphic hotsopt software being internally integrated, and show in LCD
Real-time display is for reference out on device.
Compared with prior art, the beneficial effects of the present invention are: the present invention provides a kind of temperature controls of nucleic acid augmentative instrument
Method processed reduces energy consumption by two-stage cooling piece mating reaction while guaranteeing rapid temperature rise and drop as far as possible, improves
The working efficiency of cooling piece balances the relationship between the time and efficiency of temperature adjusting, can quickly to the temperature of chip
It is adjusted, ensure that the efficient progress of PCR reaction.Meanwhile it is real to use two temperature sensors to carry out the temperature of chip
When acquire, multi-ensuring is provided, acquisition it is more accurate, the temprature control method of nucleic acid augmentative instrument provided by the invention can be right
The temperature of chip is accurately detected and controlled, and efficiently solves that existing temperature control system precision is not high, DNA fragmentation amplification
The problems such as inefficiency.
Specific embodiment
Clear, complete description is carried out to technical solution of the present invention below in conjunction with embodiment, it is clear that described reality
Applying example is a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, the common skill in this field
Art personnel every other embodiment obtained without making creative work belongs to the model that the present invention protects
Enclose, it should be noted that in the description of the present invention, the orientation or positional relationship of the instructions such as term " center " " edge " be only for
Convenient for the description present invention and simplify description, rather than the position of indication or suggestion meaning or element must have specific orientation,
It is constructed and operated in a specific orientation, therefore is not considered as limiting the invention.In addition, term " first ", " second " etc.
It is used for descriptive purposes only and cannot be understood as indicating or suggesting relative importance.
Following non-limiting embodiment can make those skilled in the art that the present invention be more completely understood, but not with
Any mode limits the present invention.Following the description be only to this application claims range exemplary illustration, this field skill
Art personnel can make a variety of changes and modification to present invention according to disclosure of that, and it should also be as belonging to this Shen
It please be among claimed range.
The present invention is further illustrated in a manner of specific embodiment below.
Embodiment
Nucleic acid augmentative instrument in the present embodiment includes the chip slot for holding chip, and chip is equipped in chip slot, is put in chip
There is sample, the cooling piece for heating and freezing to chip is used for treatment temperature for obtaining the temperature sensor of temperature signal
Signal and the master control borad for issuing temperature control command；Cooling piece includes level-one cooling piece and second level cooling piece, temperature sensor packet
Include the first temperature sensor and second temperature sensor for obtaining chip temperature signal, the first temperature sensor and the second temperature
Degree sensor is arranged between the center and peripheral of chip slot, equidistantly distributed；Temperature controlling party based on the nucleic acid augmentative instrument
Method the following steps are included:
Step 1, the Current Temperatures T and set temperature T of chip are obtained0；
The temperature signal that first temperature sensor and second temperature sensor detect is passed through signal isolation circuit respectively
It is defeated by filtering and amplifying circuit；Filtering and amplifying circuit is amplified and is filtered to temperature signal, is then transferred to A/D conversion electricity
Road, A/D conversion circuit, which is converted to temperature signal, to be transferred to master control borad after digital quantity and obtains the Current Temperatures collection value T1 of chip
And T2, T1 and T2 are collected respectively by the first temperature sensor and second temperature sensor；To temperature collection value T1 and T2 into
The Current Temperatures T of chip is obtained after row mean value；Set temperature T0To need to control the temperature that chip reaches when PCR reaction.
Step 2, the duration for presetting and heating or freezing to chip is obtained, preset value t is obtained0, t0Value 10-20s it
Between；
Step 3, control cooling piece adjusts chip temperature to set temperature, and controls heating or the cooling time t of cooling piecen
Less than or equal to preset value t0；
The concrete mode of chip temperature to set temperature is adjusted in step 3 are as follows: the processing module of master control borad is obtained by formula A
To output valve U, and output valve U is converted to the temperature adjustment signal of analog current, then exports temperature adjustment signal again to cooling piece, refrigeration
Piece carries out the adjustment of power,
Formula A:U=Ka|T-T0|+Kbd|T-T0|/dt+Kc∫|T-T0| dt, wherein U is output valve, and T is the current of chip
Temperature, T0For the set temperature of chip, KaFor proportionality coefficient, KbFor derivative time constant, KcFor integration time constant；
Cooling piece adjusts the power of cooling piece according to formula B,
Formula B:P1 or P2=CM | T-T0|/(2tnK), wherein P1 and P2 is respectively level-one cooling piece and second level cooling piece
Realtime power, C are the specific heat capacity of chip, and M is the quality of chip, and T is the Current Temperatures of chip, T0For the set temperature of chip, tn
Heating or cooling time for cooling piece, K are the heat conduction efficiency of level-one cooling piece or second level cooling piece；
In the embodiment of the present invention, Ka、KbAnd KcCan be adjusted according to the actual situation, can using experience method or
Person's empirical value, there is no a determining mathematical descriptions.And it is described in this empirical method textbook.
It can be 20 with preset temperature difference, such as preset temperature difference a in step 3 in terms of the adjustment of cooling piece power
DEG C, pass through comparison preset temperature difference a and chip Current Temperatures T and chip set temperature T0Temperature absolute value of the difference (i.e. | T-
T0|) size select the open state of level-one cooling piece and second level cooling piece；
If a >=| T-T0|, then it opens level-one cooling piece and carries out temperature adjusting；If a≤| T-T0|, then one is opened simultaneously
Grade cooling piece and second level cooling piece carry out temperature adjusting；
Amplifying nucleic acid amplification instrument of the present invention can also include LCD display, and the temperature that master control borad can be obtained according to detection is believed
Number and nucleic acid augmentative instrument operation conditions, temperature control curve is obtained by the graphic hotsopt software being internally integrated, and show in LCD
Real-time display is for reference out on device.
Obviously, above-described embodiment only clearly illustrates example, and does not limit the embodiments, this hair
The bright temperature control equipment that may be arranged as nucleic acid augmentative instrument, the module of device are documented in detail in the content of present invention,
For the those of ordinary skill in the field, other various forms of variations can also be made on the basis of the above description
Or it changes.There is no necessity and possibility to exhaust all the enbodiments.And thus amplified it is obvious variation or
It changes within still in the protection scope of the application.
Claims (10)
1. a kind of temprature control method of nucleic acid augmentative instrument, the nucleic acid augmentative instrument includes the chip for holding sample, for core
The cooling piece of piece heating and refrigeration for treatment temperature signal and issues temperature for obtaining the temperature sensor of temperature signal
The master control borad of control command, which is characterized in that the temprature control method of the nucleic acid augmentative instrument the following steps are included:
Step 1, the Current Temperatures T and set temperature T of the chip are obtained0；
Step 2, the duration for presetting and heating or freezing to the chip is obtained, preset value t is obtained0；
Step 3, control cooling piece adjusts chip temperature to set temperature, and controls heating or the cooling time t of cooling piecenIt is less than
Equal to preset value t0。
2. temprature control method according to claim 1, which is characterized in that adjust chip temperature in the step 3 to setting
Determine the concrete mode of temperature are as follows: the processing module of master control borad obtains output valve U by formula A, and output valve U is converted to simulation
Then the temperature adjustment signal of electric current exports temperature adjustment signal to cooling piece again, cooling piece carries out the adjustment of power,
Formula A:U=Ka|T-T0|+Kbd|T-T0|/dt+Kc∫|T-T0| dt, wherein U is output valve, and T is the Current Temperatures of chip,
T0For the set temperature of chip, KaFor proportionality coefficient, KbFor derivative time constant, KcFor integration time constant.
3. temprature control method according to claim 2, which is characterized in that the cooling piece includes level-one cooling piece and two
Grade cooling piece.
4. temprature control method according to claim 3, which is characterized in that cooling piece adjusts cooling piece according to formula B
Power,
Formula B:P1 or P2=CM | T-T0|/(2tnK), wherein P1 and P2 is respectively the real-time of level-one cooling piece and second level cooling piece
Power, C are the specific heat capacity of chip, and M is the quality of chip, and T is the Current Temperatures of chip, T0For the set temperature of chip, tnFor system
Cold heating or cooling time, K are the heat conduction efficiency of level-one cooling piece or second level cooling piece.
5. temprature control method according to claim 1, which is characterized in that preset value t in the step 20For 5-30s.
6. temprature control method according to claim 1, which is characterized in that the Current Temperatures T of the chip is the first temperature
The mean value of chip temperature collection value T1 and T2 that degree sensor and second temperature sensor collect.
7. temprature control method according to claim 6, which is characterized in that the nucleic acid augmentative instrument further includes holding chip
Chip slot, first temperature sensor and second temperature sensor are arranged between the center and peripheral of chip slot, and wait
Spacing distribution.
8. a kind of temperature control equipment of nucleic acid augmentative instrument, the nucleic acid augmentative instrument includes the chip for holding sample, for core
The cooling piece of piece heating and refrigeration for treatment temperature signal and issues temperature for obtaining the temperature sensor of temperature signal
The master control borad of control command, which is characterized in that the temperature control equipment of the nucleic acid augmentative instrument includes:
First acquisition unit, for obtaining the Current Temperatures T and set temperature T of the chip0；
Second acquisition unit obtains preset value t for obtaining the duration for presetting and heating or freezing to the chip0；
Control unit adjusts chip temperature to set temperature for controlling cooling piece, and heating or the cooling time t of cooling piecenIt is small
In equal to preset value t0。
9. temperature control equipment according to claim 8, which is characterized in that described control unit includes:
First computing module, for obtaining output valve U by formula (1),
U=Ka|T-T0|+Kbd|T-T0|/dt+Kc∫|T-T0|dt (1)
Wherein U is output valve, and T is the Current Temperatures of chip, T0For the set temperature of chip, KaFor proportionality coefficient, KbWhen for differential
Between constant, KcFor integration time constant；
Then first processing module exports temperature adjustment signal to system for output valve U to be converted to the temperature adjustment signal of analog current again
Cold.
10. temperature control equipment according to claim 9, which is characterized in that the cooling piece include level-one cooling piece and
Second level cooling piece, described control unit further include:
Second computing module, for obtaining the power of cooling piece by formula (2),
P1 or P2=CM | T-T0|/(2tnK) (2)
Wherein P1 and P2 is respectively the realtime power of level-one cooling piece and second level cooling piece, and C is the specific heat capacity of chip, and M is chip
Quality, T be chip Current Temperatures, T0For the set temperature of chip, tnHeating or cooling time for cooling piece, K are level-one
The heat conduction efficiency of cooling piece or second level cooling piece；
Second processing module, the power for being calculated according to the second computing module adjust the realtime power of cooling piece
Section.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110684643A (en) * | 2019-10-30 | 2020-01-14 | 宁波胤瑞生物医学仪器有限责任公司 | Temperature conduction system for nucleic acid amplification instrument |
| CN110699249A (en) * | 2019-11-01 | 2020-01-17 | 宁波胤瑞生物医学仪器有限责任公司 | Nucleic acid amplification instrument |
| CN110791422A (en) * | 2019-11-08 | 2020-02-14 | 宁波胤瑞生物医学仪器有限责任公司 | Regulating and controlling method of nucleic acid amplification instrument |
| CN110804679A (en) * | 2019-11-08 | 2020-02-18 | 宁波胤瑞生物医学仪器有限责任公司 | Method for adjusting nucleic acid amplification instrument |
| CN110804649A (en) * | 2019-11-08 | 2020-02-18 | 宁波胤瑞生物医学仪器有限责任公司 | Optical excitation and detection method of nucleic acid amplification instrument |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08285216A (en) * | 1995-04-14 | 1996-11-01 | Tlv Co Ltd | Steam temperature control device |
| CN101187816A (en) * | 2007-11-28 | 2008-05-28 | 华中科技大学 | Precision temperature controller |
| CN102147626A (en) * | 2011-04-28 | 2011-08-10 | 北京七星华创电子股份有限公司 | Feedforward temperature control method for cleaning liquid |
| CN105573368A (en) * | 2014-10-16 | 2016-05-11 | 西安扩力机电科技有限公司 | Temperature control method of PCR (Polymerase Chain Reaction) instrument |
| CN107015584A (en) * | 2017-05-16 | 2017-08-04 | 杭州电子科技大学 | A kind of temperature control device and control method applied to nucleic acid amplification technologies |
-
2019
- 2019-03-21 CN CN201910219767.6A patent/CN109976412A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08285216A (en) * | 1995-04-14 | 1996-11-01 | Tlv Co Ltd | Steam temperature control device |
| CN101187816A (en) * | 2007-11-28 | 2008-05-28 | 华中科技大学 | Precision temperature controller |
| CN102147626A (en) * | 2011-04-28 | 2011-08-10 | 北京七星华创电子股份有限公司 | Feedforward temperature control method for cleaning liquid |
| CN105573368A (en) * | 2014-10-16 | 2016-05-11 | 西安扩力机电科技有限公司 | Temperature control method of PCR (Polymerase Chain Reaction) instrument |
| CN107015584A (en) * | 2017-05-16 | 2017-08-04 | 杭州电子科技大学 | A kind of temperature control device and control method applied to nucleic acid amplification technologies |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110684643A (en) * | 2019-10-30 | 2020-01-14 | 宁波胤瑞生物医学仪器有限责任公司 | Temperature conduction system for nucleic acid amplification instrument |
| CN110684643B (en) * | 2019-10-30 | 2021-03-19 | 宁波胤瑞生物医学仪器有限责任公司 | Temperature conduction system for nucleic acid amplification instrument |
| CN110699249A (en) * | 2019-11-01 | 2020-01-17 | 宁波胤瑞生物医学仪器有限责任公司 | Nucleic acid amplification instrument |
| CN110699249B (en) * | 2019-11-01 | 2021-08-06 | 宁波胤瑞生物医学仪器有限责任公司 | Nucleic acid amplification instrument |
| CN110791422A (en) * | 2019-11-08 | 2020-02-14 | 宁波胤瑞生物医学仪器有限责任公司 | Regulating and controlling method of nucleic acid amplification instrument |
| CN110804679A (en) * | 2019-11-08 | 2020-02-18 | 宁波胤瑞生物医学仪器有限责任公司 | Method for adjusting nucleic acid amplification instrument |
| CN110804649A (en) * | 2019-11-08 | 2020-02-18 | 宁波胤瑞生物医学仪器有限责任公司 | Optical excitation and detection method of nucleic acid amplification instrument |
| CN110804679B (en) * | 2019-11-08 | 2021-02-12 | 宁波胤瑞生物医学仪器有限责任公司 | Method for adjusting nucleic acid amplification instrument |
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