CN109943342B - Weissella-based cadmium-polluted soil in-situ remediation agent - Google Patents
Weissella-based cadmium-polluted soil in-situ remediation agent Download PDFInfo
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Abstract
The invention relates to the technical field of soil remediation, and discloses a soil remediation agentThe soil remediation agent comprises a Weissella microbial inoculum, a mineral mixing agent and an activating agent, wherein the mineral mixing agent comprises the following components in parts by weight: 6-12 parts of ground phosphate rock, 6-12 parts of siliceous raw materials, 6-12 parts of quicklime, 2-8 parts of bentonite, 4-10 parts of calcium carbonate, 4-10 parts of sodium thiosulfate, 6-15 parts of activated carbon and 2-6 parts of pine soil essence, wherein the activating agent comprises the following components in parts by weight: na (Na)26-10 parts of EDTA and 10-16 parts of sodium citrate. The invention overcomes the defects of the prior art, and combines physical, chemical and biological methods to carry out multiple maintenance on the cadmium-polluted soil, thereby achieving the purpose of reducing cadmium pollution, improving the soil remediation efficiency and prolonging the remediation validity period.
Description
Technical Field
The invention relates to the technical field of soil, and particularly belongs to a weissella-based cadmium-polluted soil in-situ remediation agent.
Background
The soil environment pollution in partial areas of China is serious, the soil pollution is mainly caused by a certain pollutant, and other pollutants, namely the composite pollution problem, exist at the same time. The cultivated land area polluted by Cd, As, Cr, Pb and other heavy metals in China is about 2500 kilohm, which accounts for 1/5 of the total cultivated land area. The cadmium-lead composite pollution mainly caused by cadmium pollution seriously harms the grain yield and quality safety of main grain producing areas such as Hunan and the like in China, so the cadmium pollution of soil becomes an important environmental problem and seriously threatens the human health and the national grain safety.
At present, the treatment method aiming at the cadmium polluted soil can be mainly divided into a physical method, a chemical method, a biological method and the like. The physical method needs to consume a large amount of manpower and material resources, and the removed polluted soil is easy to cause secondary pollution. Chemical methods are easy to reactivate heavy metals, and biological methods also have limitations, such as long repair cycle and no good success in field repair.
Disclosure of Invention
The invention aims to provide a Weissella-based cadmium-polluted soil in-situ restoration agent, overcomes the defects of the prior art, and combines physical, chemical and biological methods to carry out multiple maintenance on cadmium-polluted soil to achieve the aim of reducing cadmium pollution, improve the restoration efficiency of the soil and prolong the restoration period of validity.
In order to solve the problems, the technical scheme adopted by the invention is as follows:
the cadmium-contaminated soil in-situ restoration agent based on Weissella is characterized by comprising a Weissella microbial inoculum, a mineral mixing agent and an activating agent, wherein the mineral mixing agent comprises the following components in parts by weight: 6-12 parts of ground phosphate rock, 6-12 parts of siliceous raw materials, 6-12 parts of quicklime, 2-8 parts of bentonite, 4-10 parts of calcium carbonate, 4-10 parts of sodium thiosulfate, 6-15 parts of activated carbon, 2-6 parts of pine essence and 20-40 parts of deionized water, wherein the activating agent comprises the following components in parts by weight: na (Na)26-10 parts of EDTA, 10-16 parts of sodium citrate and 20-40 parts of distilled water.
Further, the soil remediation agent comprises a Weissella microbial inoculum, a mineral mixing agent and an activating agent, wherein the mineral mixing agent comprises the following components in parts by weight: 9 parts of ground phosphate rock, 9 parts of siliceous raw materials, 9 parts of quicklime, 5 parts of bentonite, 7 parts of calcium carbonate, 7 parts of sodium thiosulfate, 11 parts of activated carbon, 4 parts of scarifying agent and 30 parts of deionized water, wherein the activating agent comprises the following components in parts by weight: na (Na)28 parts of EDTA, 13 parts of sodium citrate and 30 parts of distilled water.
Further, the Weissella bacterial agent adopts a strain Weissella virescens ZY-6 with a preservation number of GDMCC NO: 60377.
further, the mass ratio of the phosphorus ore powder, the siliceous raw material and the quicklime in the mineral mixture is 1:1:1, and the siliceous raw material is any one or a mixture of any several of silicon dioxide, sodium silicate or diatomite.
Furthermore, the activated carbon is screened by a 100-mesh screen to obtain the ultrafine-grained activated carbon.
Further, the preparation method of the soil remediation agent comprises the following specific steps:
(1) separating and screening cadmium-resistant Weissella strain from old pickle juice of Sichuan, inoculating the primarily screened cadmium-resistant strain to the original pickle juiceIn Cd2+In a solid culture medium with higher concentration, the high concentration Cd can be selected2+And (4) streaking and purifying the colonies growing on the plate to obtain the target Weissella strain. Extracting Weissella strain, and diluting to concentration of 4.0x107～8.0x107Per mL, the Weissella bacterial agent is obtained;
(2) according to the mass parts, taking 6-12 parts of ground phosphate rock, 6-12 parts of siliceous raw materials, 6-12 parts of quicklime, 2-8 parts of bentonite, 4-10 parts of calcium carbonate, 4-10 parts of sodium thiosulfate, 6-15 parts of activated carbon, 2-6 parts of pine essence and 20-40 parts of deionized water, carrying out ball milling, drying, carrying out heat preservation, and cooling to room temperature to obtain a mineral mixture;
(3) taking 6-10 parts of Na by mass2EDTA, 10-16 parts of sodium citrate and 20-40 parts of distilled water, and stirring and mixing at 37-40 ℃ for 2-3h to obtain the active agent.
Further, the using method of the soil remediation agent comprises the following steps:
(1) uniformly spreading the Weissella microbial inoculum into the cadmium-polluted soil according to the application amount of the Weissella microbial inoculum to the cadmium-polluted soil in a weight ratio of 2-12:100, turning over and maintaining for 3-5 days;
(2) spreading 1-3 cm of mineral mixture on the surface of the cadmium-polluted soil, ploughing, and maintaining for 10-15 days;
(3) uniformly spreading the active agent into the cadmium-polluted soil according to the application amount of the active agent to the cadmium-polluted soil in a weight ratio of 10-20:100, ploughing and maintaining for 3-6 days.
Furthermore, the number of times of each turn is not less than two, and the turn depth is not less than 20 cm.
Compared with the prior art, the invention has the following implementation effects:
compared with the prior art, the cadmium-polluted soil in-situ restoration agent based on Weissella has the advantages that:
1. the Weissella strain is used as a main material of the repairing agent, and is separated and screened from the old pickle juice in Sichuan, so that the Weissella strain has high tolerance and high adsorption capacity on cadmium, has high bioremediation capacity, improves the repairing efficiency of the repairing agent on cadmium soil, and has the advantages of low cost, good effect, high yield and the like.
2. The invention adopts mineral substances, can effectively reduce the content of cadmium in soil, has no harm to the environment, is green and environment-friendly, has simple manufacturing process and low manufacturing cost, and prolongs the effective period of restoration.
3. The soil restoration method adopts the activating agent for restoration, the activating agent is a main mode of detoxification of heavy metals in cells by plants, and the activating agent helps plants planted in the soil to detoxify, prolongs the effective period of soil restoration, and restores the activity of the soil.
4. The invention combines physical, chemical and biological methods to carry out multiple maintenance on the cadmium-polluted soil, thereby achieving the purpose of reducing cadmium pollution, improving the soil remediation efficiency and prolonging the remediation validity period
Detailed Description
The present invention will be further described with reference to the following examples, but the present invention is not limited to these examples, and any modification is within the scope of the present invention without departing from the spirit of the present invention.
Example 1
The invention relates to a Weissella-based cadmium-polluted soil in-situ restoration agent,
preparing a soil remediation agent:
(1) separating and screening cadmium-resistant Weissella strain from old pickle juice of Sichuan, inoculating the primarily screened cadmium-resistant strain to Cd2+In a solid culture medium with higher concentration, the high concentration Cd can be selected2+And (4) streaking and purifying the colonies growing on the plate to obtain the target Weissella strain. Extracting Weissella strain, and diluting to concentration of 4.0x107Per mL, the Weissella bacterial agent is obtained;
(2) taking 6 parts of ground phosphate rock, 6 parts of siliceous raw materials, 6 parts of quicklime, 2 parts of bentonite, 4 parts of calcium carbonate, 4 parts of sodium thiosulfate, 6 parts of active carbon, 2 parts of pine essence and 20 parts of deionized water, ball-milling, drying, preserving heat and cooling to room temperature to obtain a mineral mixture;
(3) according to the mass portionCounting, taking 6 parts of Na2EDTA, 10 parts of sodium citrate and 20 parts of distilled water are stirred and mixed for 2 hours at the temperature of 37-40 ℃ to obtain the active agent.
The soil restoration treatment method comprises the following steps:
(1) uniformly spreading the Weissella microbial inoculum into the cadmium-polluted soil according to the application amount of the Weissella microbial inoculum to the cadmium-polluted soil in a weight ratio of 2:100, turning over and maintaining for 3 days;
(2) spreading a mineral mixture of 3 cm on the surface of the cadmium-polluted soil, ploughing and maintaining for 10 days;
(3) uniformly spreading the active agent into the cadmium-polluted soil according to the application amount of the active agent to the cadmium-polluted soil in a weight ratio of 10:100, ploughing and maintaining for 3 days.
And after the maintenance is finished, collecting a soil sample for analysis, reducing the cadmium content in the soil sample to 0.8mg/kg to 0.12mg/kg, wherein the reduction amplitude reaches 85%, realizing the in-situ remediation of the cadmium-polluted soil, and the soil quality after the remediation is close to that of natural soil.
Example 2
The invention relates to a Weissella-based cadmium-polluted soil in-situ remediation agent, which is prepared by the following steps:
(1) separating and screening cadmium-resistant Weissella strain from old pickle juice of Sichuan, inoculating the primarily screened cadmium-resistant strain to Cd2+In a solid culture medium with higher concentration, the high concentration Cd can be selected2+And (4) streaking and purifying the colonies growing on the plate to obtain the target Weissella strain. Extracting Weissella strain, and diluting to concentration of 6.0x107Per mL, the Weissella bacterial agent is obtained;
(2) according to the mass parts, 9 parts of ground phosphate rock, 9 parts of siliceous raw materials, 9 parts of quicklime, 5 parts of bentonite, 7 parts of calcium carbonate, 7 parts of sodium thiosulfate, 11 parts of activated carbon, 4 parts of pine essence and 30 parts of deionized water are taken, ball-milled, dried, kept warm and cooled to room temperature to obtain a mineral mixture;
(3) taking 8 parts of Na by weight2EDTA, 13 parts of sodium citrate and 30 parts of distilled water are stirred and mixed for 2.5 hours at the temperature of 37-40 ℃ to obtain the active agent.
The soil restoration treatment method comprises the following steps:
(1) uniformly spreading the Weissella microbial inoculum into the cadmium-polluted soil according to the application amount of the Weissella microbial inoculum to the cadmium-polluted soil in a weight ratio of 7:100, turning over and maintaining for 4 days;
(2) spreading a mineral mixture of 3 cm on the surface of the cadmium-polluted soil, ploughing and maintaining for 13 days;
(3) uniformly spreading the active agent into the cadmium-polluted soil according to the application amount of the active agent to the cadmium-polluted soil in a weight ratio of 10:100, ploughing and maintaining for 4 days.
And after the maintenance is finished, collecting a soil sample for analysis, reducing the cadmium content in the soil sample to 0.8mg/kg to 0.08mg/kg, wherein the reduction amplitude reaches 90%, realizing the in-situ remediation of the cadmium-polluted soil, and the soil quality after the remediation is close to that of natural soil.
Example 3
(1) Separating and screening cadmium-resistant Weissella strain from old pickle juice of Sichuan, inoculating the primarily screened cadmium-resistant strain to Cd2+In a solid culture medium with higher concentration, the high concentration Cd can be selected2+And (4) streaking and purifying the colonies growing on the plate to obtain the target Weissella strain. Extracting Weissella strain, and diluting to 8.0x107Per mL, the Weissella bacterial agent is obtained;
(2) according to the mass parts, taking 12 parts of ground phosphate rock, 12 parts of siliceous raw materials, 12 parts of quicklime, 8 parts of bentonite, 10 parts of calcium carbonate, 10 parts of sodium thiosulfate, 15 parts of activated carbon, 6 parts of pine essence and 40 parts of deionized water, carrying out ball milling, drying, carrying out heat preservation, and cooling to room temperature to obtain a mineral mixture;
(3) taking 10 parts of Na by weight2EDTA, 16 parts of sodium citrate and 40 parts of distilled water are stirred and mixed for 3 hours at the temperature of 37-40 ℃ to obtain the active agent.
The soil restoration treatment method comprises the following steps:
(1) uniformly spreading the Weissella microbial inoculum into the cadmium-polluted soil according to the application amount of the Weissella microbial inoculum to the cadmium-polluted soil in a weight ratio of 12:100, turning over and maintaining for 5 days;
(2) spreading a mineral mixture of 3 cm on the surface of the cadmium-polluted soil, ploughing and maintaining for 15 days;
(3) uniformly spreading the active agent into the cadmium-polluted soil according to the application amount of 12:100 of the weight ratio of the active agent to the cadmium-polluted soil, ploughing and maintaining for 4 days.
After the maintenance is finished, a soil sample is collected for analysis, the cadmium content in the soil sample is reduced to 0.06mg/kg from 0.8mg/kg, the reduction amplitude reaches 92.5 percent, the in-situ remediation of the cadmium-polluted soil is realized, and the soil quality after the remediation is close to that of natural soil.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.
The foregoing is merely exemplary and illustrative of the present inventive concept and various modifications, additions and substitutions of similar embodiments may be made to the specific embodiments described by those skilled in the art without departing from the inventive concept or exceeding the scope of the claims as defined in the accompanying claims.
Claims (7)
1. The cadmium-contaminated soil in-situ restoration agent based on Weissella is characterized by comprising a Weissella microbial inoculum, a mineral mixing agent and an activating agent, wherein the mineral mixing agent comprises the following components in parts by weight: 6-12 parts of ground phosphate rock, 6-12 parts of siliceous raw material, 6-12 parts of quicklime, 2-8 parts of bentonite, 4-10 parts of calcium carbonate and sodium thiosulfate4-10 parts of activated carbon, 6-15 parts of pine soil essence and 20-40 parts of deionized water, wherein the activating agent comprises the following components in parts by weight: na (Na)26-10 parts of EDTA, 10-16 parts of sodium citrate and 20-40 parts of distilled water;
the using method of the soil remediation agent comprises the following steps:
(1) uniformly spreading the Weissella microbial inoculum into the cadmium-polluted soil according to the application amount of the Weissella microbial inoculum to the cadmium-polluted soil in a weight ratio of 2-12:100, turning over and maintaining for 3-5 days;
(2) spreading 1-3 cm of mineral mixture on the surface of the cadmium-polluted soil, ploughing, and maintaining for 10-15 days;
(3) uniformly spreading the active agent into the cadmium-polluted soil according to the application amount of the active agent to the cadmium-polluted soil in a weight ratio of 10-20:100, ploughing and maintaining for 3-6 days.
2. The weissella-based cadmium-contaminated soil in-situ remediation agent of claim 1, wherein the soil remediation agent comprises a weissella microbial inoculum, a mineral mix and an activator, wherein the mineral mix comprises the following components in parts by weight: 9 parts of ground phosphate rock, 9 parts of siliceous raw materials, 9 parts of quicklime, 5 parts of bentonite, 7 parts of calcium carbonate, 7 parts of sodium thiosulfate, 11 parts of activated carbon, 4 parts of scarifying agent and 30 parts of deionized water, wherein the activating agent comprises the following components in parts by weight: na (Na)28 parts of EDTA, 13 parts of sodium citrate and 30 parts of distilled water.
3. The Weissella-based cadmium-contaminated soil in-situ remediation agent of claim 1, wherein the Weissella inoculum adopts a strain Weissella virescens ZY-6 with a deposit number GDMCC NO: 60377.
4. the weissella-based cadmium-polluted soil in-situ restoration agent according to claim 1, wherein the mass ratio of the phosphate ore powder, the siliceous raw material and the quicklime in the mineral mixture is 1:1:1, and the siliceous raw material is any one or a mixture of any several of silicon dioxide, sodium silicate or diatomite.
5. The weissella-based cadmium-contaminated soil in-situ remediation agent as claimed in claim 1, wherein the activated carbon is ultrafine-grained activated carbon obtained after being screened by a 100-mesh screen.
6. The weissella-based cadmium-contaminated soil in-situ remediation agent of claim 1, wherein the soil remediation agent is prepared by the following specific steps:
(1) separating and screening cadmium-resistant Weissella strain from old pickle juice of Sichuan, inoculating the primarily screened cadmium-resistant strain to Cd2+In a solid culture medium with higher concentration, the high concentration Cd can be selected2+And (4) streaking and purifying the colonies growing on the plate to obtain the target Weissella strain. Extracting Weissella strain, and diluting to concentration of 4.0x107～8.0x107Per mL, the Weissella bacterial agent is obtained;
(2) according to the mass parts, taking 6-12 parts of ground phosphate rock, 6-12 parts of siliceous raw materials, 6-12 parts of quicklime, 2-8 parts of bentonite, 4-10 parts of calcium carbonate, 4-10 parts of sodium thiosulfate, 6-15 parts of activated carbon, 2-6 parts of pine essence and 20-40 parts of deionized water, carrying out ball milling, drying, carrying out heat preservation, and cooling to room temperature to obtain a mineral mixture;
(3) taking 6-10 parts of Na by mass2EDTA, 10-16 parts of sodium citrate and 20-40 parts of distilled water, and stirring and mixing at 37-40 ℃ for 2-3h to obtain the active agent.
7. The weissella-based cadmium contaminated soil in-situ remediation agent of claim 1, wherein the number of plowing is not less than two times per turn, and the depth of plowing is not less than 20 cm.
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