CN109929756B - Ultrasonic microbubble cell stimulation device and operation method thereof - Google Patents
Ultrasonic microbubble cell stimulation device and operation method thereof Download PDFInfo
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- CN109929756B CN109929756B CN201711349155.6A CN201711349155A CN109929756B CN 109929756 B CN109929756 B CN 109929756B CN 201711349155 A CN201711349155 A CN 201711349155A CN 109929756 B CN109929756 B CN 109929756B
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Abstract
The invention is applicable to the field of medical instruments, and provides an ultrasonic microbubble cell stimulation device and an operation method thereof. The ultrasonic microbubble cell stimulation device comprises a culture dish, a positioning frame and a base, wherein a culture hole is formed in the culture dish, a sealing film is arranged on an opening end of the culture hole, and a culture sleeve is arranged in the culture hole; the base is connected with a conduction assembly which is used for conducting ultrasonic waves to stimulate cells and can adjust the distance between the base and the culture dish; the base is connected with the positioning frame through the positioning component. The operating method comprises the steps of guide rod installation, conducting assembly connection and assembly, positioning frame installation, culture sleeve installation, microbubble injection and culture dish installation. The device can effectively prevent air bubbles from being mixed in the culture dish, ensures that the ultrasound can directly act on the microbubbles, and has controllable area and strength of stimulation action, simple and convenient operation method, good universality and wide application range.
Description
Technical Field
The invention belongs to the field of medical instruments, and particularly relates to an ultrasonic microbubble cell stimulation device and an operation method thereof.
Background
With the development of science and technology, the cognition of ultrasonic diagnosis and treatment is continuously deepened, and the research of ultrasonic stimulation becomes a hot spot of the current medical treatment. Ultrasound stimulation is gaining increasing attention due to its safety, non-invasiveness, effectiveness, and real-time. In the world, many laboratories are currently conducting research on ultrasonic stimulation of cells, and ultrasonic stimulation microbubbles (or drug-loaded microbubbles) act on cells or lesions to achieve a more ideal effect. However, the microbubbles need to act on the cells at the bottom of the dish, so the microbubbles must float to the cells at the bottom of the dish by inverting the dish, and the air enters the dish by sealing the dish and inverting the sealed membrane, so that the ultrasound cannot act on the microbubbles and stimulate the cells.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides an ultrasonic microbubble cell stimulation device and an operation method thereof.
The invention is realized in the following way: an ultrasonic microbubble cell stimulation device comprises a culture dish for cell culture, a positioning frame for bearing and placing the culture dish and a base for supporting the positioning frame, wherein a culture hole is formed in the culture dish, a sealing film is arranged on an opening end of the culture hole, and a culture sleeve which is used for injecting microbubbles into the culture hole and can prevent air from being mixed with the microbubbles after the culture dish is sealed and inverted by the sealing film is arranged in the culture hole; the base is connected with a conduction assembly which is used for conducting ultrasonic waves to stimulate cells and can adjust the distance between the base and the culture dish; the base is connected with the positioning frame through a positioning component.
Optionally, the culture sleeve includes a barrel with openings at two ends, one end of the barrel is connected with a blocking head for blocking air from entering the barrel, the blocking head is provided with an injection hole for injecting the microbubbles into the barrel, and the other end of the opening of the barrel is inserted into the culture hole.
Preferably, the axial section of the blocking head is in a conical shape, and the injection hole is formed in the top of the blocking head.
Optionally, a connection hole for positioning and connecting the conduction assembly is formed in the base, and one end of the conduction assembly is arranged in the connection hole.
Furthermore, a positioning step is further arranged in the connecting hole.
Optionally, the conducting assembly includes a probe and a collimator, one end of the probe is inserted into the connecting hole and abuts against the positioning step, the collimator is sleeved on the probe and provided with a connecting structure therebetween, and one end of the collimator opposite to the culture dish is provided with a sound-transmitting membrane.
Optionally, the connecting structure includes an external thread arranged on the outer wall of the probe and an internal thread correspondingly arranged on the inner wall of the collimator, and the external thread is meshed with the internal thread.
Optionally, the positioning assembly includes a guide rod disposed on the base and a guide hole disposed on the positioning frame, one end of the guide rod is fixed on the base, and the other end of the guide rod movably penetrates through the guide hole and is disposed with a positioning structure for positioning and adjusting the position therebetween.
Optionally, the positioning structure includes a threaded hole formed in the positioning frame and a locking member for abutting against the guide rod, the threaded hole is perpendicular to the axial direction of the guide hole, and the locking member is in threaded connection with the threaded hole.
Optionally, a plurality of culture holes are formed in the culture dish in an array mode, and a plurality of connecting holes are correspondingly formed in the base.
Optionally, the locating rack includes the support body, offer the confession on the support body the culture dish holds the holding tank of placing, and can the reciprocating motion positioning, the bottom of holding tank is offered and is used for supplying the collimator pass with the opening of culture cover contact effect.
Optionally, a positioning member for positioning the adjusting position of the culture dish is arranged on the positioning frame.
The invention also provides an operation method of the ultrasonic microbubble cell stimulation device, which adopts the ultrasonic microbubble cell stimulation device and comprises the following steps:
the guide rod mounting step: one end of the guide rod is fixedly connected to the base;
the conductive component connecting and assembling step: mounting the probe on the base and connecting the collimator to the probe;
the positioning frame mounting step: the other end of the guide rod penetrates through the guide hole, and the position of the positioning frame on the guide rod is locked through the locking piece;
the culture sleeve installation and the microbubble injection step: the culture sleeve is inserted into the culture hole, the microvesicles are injected into the culture sleeve through the injection hole, and finally the open end of the culture dish is sealed through the sealing membrane.
The culture dish installation step: the sealed culture dish is inverted in the accommodating groove, the placing position is adjusted, the culture holes correspond to the connecting holes one to one, and then the position of the culture dish is fixed through the positioning piece.
Optionally, in the positioning frame mounting step, after the positioning frame is connected to the guide rod, the connection position of the positioning frame is adjusted, so that the bottom surface of the positioning frame is parallel to the upper surface of the base.
Optionally, in the step of installing the culture sleeve and injecting the microvesicles, after the culture sleeve is installed in the culture hole, the open end of the culture sleeve is in contact with the bottom of the culture dish, and the top of the blocking head is parallel to the upper surface of the culture dish.
The invention provides an ultrasonic microbubble cell stimulation device and an operation method thereof. Therefore, when the culture dish is packaged, the culture sleeve can not only meet the injection of the microbubbles, but also block mixed air outside the culture sleeve, effectively solves the problem that the microbubbles in the air and the culture sleeve are mixed, and further achieves the purpose of reliably stimulating the microbubbles by ultrasonic. And through the degree of depth of adjusting conduction subassembly and the height of locating rack, alright in order to control the area size and the intensity that the cell received even supersound stimulation in the culture dish, the commonality is good. The device for stimulating the cells by the ultrasonic microbubbles is simple in structure, simple in operation method and steps, wide in application range and capable of improving convenience of experiment operation.
Drawings
FIG. 1 is a schematic diagram of an assembly of an ultrasonic microbubble cell stimulation device provided by an embodiment of the present invention;
FIG. 2 is an exploded view of an ultrasound microbubble cell stimulation device provided by an embodiment of the present invention;
FIG. 3 is a schematic view of the installation of the base and the guide bar provided by the embodiment of the present invention;
FIG. 4 is a schematic view of the probe and base assembly provided by the embodiment of the present invention;
FIG. 5 is a schematic view of an installation of a collimator and a probe according to an embodiment of the present invention;
FIG. 6 is a schematic view of the positioning frame and the guide bar according to the embodiment of the present invention;
FIG. 7 is a schematic view of the assembly of the culture jacket and the culture dish according to the embodiment of the invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
The following detailed description of specific implementations of the present invention is provided in conjunction with specific embodiments:
as shown in fig. 1 to 7, an ultrasound microbubble cell stimulation device provided in the embodiment of the present invention includes a culture dish 11 for cell culture, a positioning frame for carrying and placing the culture dish 11, and a base 13 for supporting the positioning frame 12. A culture hole 14 is arranged in the culture dish 11, a sealing film 10 is arranged on the opening end, and a culture sleeve 15 which is used for injecting the micro-bubble into the culture hole 14 and can prevent the air from mixing with the micro-bubble after the culture dish 11 is sealed and inverted by the sealing film 10 is arranged in the culture hole 14; a conduction assembly 16 which is used for conducting ultrasonic waves to stimulate cells and can adjust the distance from the culture dish 11 is connected to the base 13; the base 13 and the positioning frame 12 are connected through a positioning component 17. Thus, in the experiment, the culture hole 14 is cultured with cells and filled with culture solution, after the culture sleeve 15 is placed in the culture hole 14, the culture sleeve 15 is soaked in the culture solution, and then the microbubbles are injected into the culture sleeve 15, and the arrangement of the culture sleeve 15 does not affect the culture and stimulation of the cells, and the mixed air can be blocked outside the culture sleeve 15 when the opening end of the culture dish 11 is sealed by the sealing film 10 through the blocking of the culture sleeve 15, so that when the culture dish 11 is inverted for ultrasonic stimulation, the problem that the mixed air bubbles affect the ultrasonic waves to act on the microbubbles and stimulate the cells can be avoided. This culture sleeve 15's setting has realized completely cutting apart the air bubble outside culture sleeve 15, has satisfied the user demand to the supersound stimulation cell in the middle of the experiment effectively. And through the conduction subassembly 16 that is provided with, can independently adjust the length of self, ensure to realize reliable contact with the culture cover 15 that corresponds, guarantee that the ultrasonic wave can act on the cell of culture dish 11 bottom, combine the positioning subassembly 17 that sets up simultaneously, adjust the distance between culture dish 11 and the base 13. Thus, the area and intensity of the cells in the culture dish 11 which are subjected to uniform ultrasonic stimulation can be controlled, and the universality is wider.
Alternatively, as shown in fig. 1 and 7, when the sealing of the open end of the culture dish 11 is actually performed, air bubbles are inevitably introduced into the inner surface of the sealing film 10 during the sealing process, and due to the culture sleeve 15, the air bubbles are only present on the outer side of the culture sleeve 15 after the culture dish 11 is inverted, and the air bubbles do not enter the inside of the culture sleeve 15 and mix with the microbubbles. Therefore, the culture medium bag 15 is provided to include a cylindrical body 151 having both ends opened, and a blocking head 152 for blocking air from entering the cylindrical body 151 is connected to any one end of the cylindrical body 151, and the blocking head 152 is opened with an injection hole 153 for injecting microbubbles into the cylindrical body 151, and the other open end of the cylindrical body 151 is inserted into the culture hole 14. Set up like this, separation head 152 can be sealed with the one end that self set up, and separation head 152's shape is not restricted, only need ensure to pack into in the cultivation hole 14 back, there is the clearance with the inner wall of cultivation hole 14, thereby there is the effect that can play the holding air bubble through this clearance space, and the size of setting injection hole 153 aperture on separation head 152, should be satisfying under the prerequisite that the experiment was injected into the microbubble operation demand, can reduce as far as possible, thereby can reduce the air bubble and mix into the possibility in the cultivation cover 15 from this injection hole 153. The culture sleeve 15 is simple in structure and ingenious in arrangement, and can reliably and effectively solve the problem of mixing of air bubbles and micro bubbles.
Preferably, in the present embodiment, the blocking head 152 is configured in a shape that is "tapered" in axial cross-section and opens the injection hole 153 at the top of the blocking head 152. Thus, the tapered blocking head 152 can form a large space for accommodating air bubbles with the inner wall of the culture well 14, and has a good effect of blocking the air bubbles from entering the culture sleeve 15 when the culture dish 11 is sealed and turned upside down. Of course, it is understood that the shape of the blocking head 152 is not limited to a cone, but may be other shapes such as a sphere or a semi-ellipse, which can form a gap with the sidewall of the culture well 14.
Alternatively, in the actual use process, the conducting component 16 can be directly placed on the base 13, and the position is kept stable without shaking. In the embodiment of the present invention, as shown in fig. 1 to 7, in order to ensure the stability of the position after the installation and connection between the conducting component 16 and the base 13, a connection hole 131 for positioning and connecting the conducting component 16 is provided on the base 13, and one end of the conducting component 16 is disposed in the connection hole 131. Thus, the position limiting function of the connecting hole 131 can play a role in preventing the position of the conduction assembly 16 from being changed, and also facilitates the alignment of the contact position between the culture sleeve 15 and the conduction assembly 16, thereby meeting the use requirement that the axes of the corresponding culture hole 14 and the connecting hole 131 are kept on the same straight line in the process of experiments. Moreover, in order to improve the convenience of connection between the conduction assembly 16 and the connection hole 131 and the stability of the position after connection, the connection mode between the conduction assembly 16 and the connection hole 131 may be a structure form capable of realizing quick installation and disassembly, such as a threaded connection or a snap connection.
Further, as shown in fig. 1 to 7, in order to improve the convenience of positioning and mounting the conductive member 16, a positioning step 132 is further provided in the connection hole 131. The setting of this location step 132, need guarantee with base 13 upper surface's flatness and depth of parallelism to ensure the accuracy of conduction subassembly 16 position after the installation, and then can ensure the depth of parallelism of the contact surface between conduction subassembly 16 and culture cover 15, be favorable to the conduction of ultrasonic wave.
Alternatively, as shown in fig. 1 to 7, in the embodiment of the present invention, the conducting assembly 16 includes a probe 161 and a collimator 162, one end of the probe 161 is inserted into the connecting hole 131 and abuts against the positioning step 132, so that the connection stability is good, and the parallelism between the probe 161 and the culture dish 11 is also ensured. The collimator 162 is sleeved on the probe 161, and a connecting structure 18 is arranged between the collimators. Through this connection structure 18, not only can satisfy the demand of being connected between probe 161 and the collimator 162, moreover through this connection structure 18, can realize adjusting the position of collimator 162 on probe 161, and then can satisfy the user demand of adjusting the probe 161 apart from the culture dish 11 degree of depth. And the end of the collimator 162 opposite to the culture dish 11 is provided with an acoustic membrane 163, and the couplant is encapsulated in the cavity of the collimator 162 through the acoustic membrane 163, so that the ultrasound can act on the bottom of the culture dish 11 to stimulate the cells, thereby achieving the purpose of experiment.
Preferably, the probe 161 is a plane or focusing probe 161, and the shape and size of the collimator 162 are not limited, so long as the collimator can be matched with the corresponding probe 161.
Alternatively, as shown in fig. 1-7, the connecting structure 18 includes external threads 181 disposed on the outer wall of the probe 161 and corresponding internal threads (not shown) disposed on the inner wall of the collimator 162, the external threads 181 engaging the internal threads. Like this, realize being connected between probe 161 and the collimater 162 through the mode that sets up helicitic texture, not only connect the convenience, the degree of depth of also being convenient for adjust the hookup location moreover has improved the convenience of operation in the experimentation. Of course, the connecting structure 18 may also be a combination of a gear and a rack, and the adjustment of the connecting position can be realized by adjusting the teeth at different positions on the gear and the rack to realize meshing and limiting and fixing the gear to be incapable of rotating after adjustment.
Alternatively, as shown in fig. 1 to 7, in the embodiment of the present invention, the positioning assembly 17 includes a guide rod 171 disposed on the base 13 and a guide hole 172 disposed on the positioning frame 12, one end of the guide rod 171 is fixed on the base 13, and the other end thereof movably penetrates through the guide hole 172 and a positioning structure for positioning and adjusting the position is disposed therebetween. Like this, under guide arm 171 and guide hole 172 mutually support and the guide effect, can reliably realize adjusting the operation of locating rack 12 position height on guide arm 171, and then can realize adjusting the distance between locating rack 12 and the collimater 162, and after adjusting through location structure 19 with the rigidity of locating rack 12, thereby can control the amazing area size and the intensity of ultrasonic wave that the cell received in the culture dish 11, satisfy the user demand under the different experimental conditions, the commonality is more extensive. And the guide rod 171 is used for positioning and moving, so that the position is accurate, the stress is balanced, and the operation is simple and convenient. The guide rods 171 are provided to guide the movement of the positioning frame 12, and the positioning frame 12 can be moved up and down without being shifted in the front-rear direction or rotated by the guide rods 171, thereby improving the reliability of use. The number of the guide rods 171 provided on the base 13 may be determined according to specific requirements, and the stability of the connection position of the positioning frame 12 is ensured, which is not limited herein.
Alternatively, as another implementation manner of the structure of the positioning assembly 17, the positioning assembly 17 may be configured as a combination of a screw and a threaded hole, and then the height of the connection position of the positioning frame 12 is adjusted by rotating the screw, and the manner of driving the screw to rotate may be manual rotation or motor driving. The structure of the positioning component 17 can also be a combination of a screw and a nut, namely, the screw is fixedly connected to the base 13 and screwed on the screw, the positioning frame 12 is directly sleeved on the screw, and then the height of the positioning frame 12 is adjusted by screwing the nut, so that the use requirement of position adjustment can be met.
Alternatively, as shown in fig. 1 to 7, in the embodiment of the present invention, the positioning structure 19 includes a threaded hole 191 opened on the positioning frame 12 and a locking member 192 for abutting against the guide rod 171, the threaded hole 191 is disposed perpendicular to the axial direction of the guide hole 172, and the locking member 192 is screwed in the threaded hole 191. That is, the locking member 192 is screwed to abut against the corresponding guide rod 171, so as to prevent the position of the positioning frame 12 from being changed, thereby achieving the positioning and locking of the connecting position of the positioning frame 12, and the positioning structure 19 is convenient to operate, stable and reliable. Of course, it is understood that the positioning structure 19 may be implemented as an elastic latch or a snap ring.
Optionally, a plurality of culture holes 14 are arranged in the culture dish 11 in an array, and a plurality of connecting holes 131 are correspondingly arranged on the base 13. Like this, can carry out cell culture in every culture hole 14 simultaneously to carry out the ultrasonic stimulation to the cell in every culture hole 14 simultaneously through being provided with conduction component 16 on every connecting hole 131 on base 13, can not only improve the efficiency of experiment, the experiment is more convenient and fast, and the contrast is stronger moreover. Alternatively, stimulating cells in different culture wells 14 by a single conductive element 16 may be accomplished by using a single conductive element 16 and then shifting the position of the culture dish 11 or the position of the conductive element 16.
Alternatively, as shown in FIG. 7, the culture wells 14 disposed in the culture dish 11 are disposed in an equally spaced manner, and adjacent culture wells 14 are fixedly connected to each other by a connecting plate 111 to ensure the structural stability of the culture wells 14.
Alternatively, in a specific arrangement, the culture well 14, the connection hole 131, the probe 161 and the collimator 162 are shaped to match each other, for example, to have a horizontal cross section of "quadrangle" or "pentagon", and in the embodiment of the present invention, the shape is "circle".
Optionally, as shown in fig. 1 to 7, in the embodiment of the present invention, the positioning frame 12 includes a frame body 121, a receiving groove 122 for receiving and placing the culture dish 11 and capable of moving back and forth and adjusting is formed on the frame body 121, and an opening 123 for allowing the collimator 162 to pass through and contact with the culture sleeve 15 is formed at the bottom of the receiving groove 122. Like this, place back in holding tank 122 with culture dish 11, just can remove and realize the adjustment of supersound stimulation position of acting on in holding tank 122, satisfy the demand of position adjustment in the experimentation. The bottom opening 123 of the receiving groove 122 is preferably sized to allow all the connecting holes 131 of the base 13 and all the culture holes 14 of the culture dish 11 to be connected with each other. And the width of the receiving groove 122 may be slightly larger than the width of the opening 123, so that supporting slide rails can be formed at both sides of the width direction of the opening 123 to support and guide the culture dish 11 to slide.
Alternatively, as shown in fig. 1 and 6, after the position of the culture dish 11 is adjusted, in order to ensure the stability of the position of the culture dish 11, a positioning member 121 for positioning the adjusted position of the culture dish 11 is provided on the positioning frame 12. Thus, the positioning member 124 abuts against the culture dish 11 to lock the position of the culture dish 11, and the operation is convenient.
According to the ultrasonic microbubble cell stimulation device provided by the embodiment of the invention, the culture sleeve 15 is arranged in the culture hole 14, so that when the culture dish 11 is packaged and inverted, not only can the injection of microbubbles be met, but also the mixed air can be blocked outside the culture sleeve 15 through the culture sleeve 15, the problem that the air is mixed with the microbubbles in the culture sleeve 15 is effectively solved, and the purpose of reliably ultrasonically stimulating the microbubbles is further achieved. Meanwhile, the size and the strength of the area of the cells in the culture dish 11 which are stimulated by the uniform ultrasonic can be controlled by adjusting the depth of the collimator 162 and the height of the positioning frame 12, and the universality is good. The device for stimulating the cells by the ultrasonic microbubbles is simple in structure, simple in operation method and steps, wide in application range and capable of improving convenience of experiment operation.
The embodiment of the invention also provides an operation method of the ultrasonic microbubble cell stimulation device, which adopts the ultrasonic microbubble cell stimulation device and comprises the following steps:
the guide rod 171 mounting step: one end of the guide rod 171 is fixedly coupled to the base 13.
Mounting the positioning frame 12: the other end of the guide rod 171 passes through the guide hole 172 and, after the positional adjustment of the spacer 12 is completed, the position of the spacer 12 on the guide rod 171 is locked by the locking member 192.
Alternatively, in the above steps of the operation method, the conductive member 16 may be mounted on the base 13 after the positioning frame 12 is connected to the guide rods 171.
Alternatively, in the positioning frame 12 mounting step, after the positioning frame 12 is attached to the guide rods 171, the attachment position of the positioning frame 12 needs to be adjusted so that the bottom surface of the positioning frame 12 is parallel to the upper surface of the base 13, and the position of the positioning frame 12 needs to be adjusted so that the top surface of the sound-permeable membrane 163 on the collimator 162 is parallel to the surface of the opening 123 on the positioning frame 12, thereby ensuring that the sound-permeable membrane 163 can be attached to the sealing membrane 10 on the culture dish 11.
Alternatively, in the step of installing the culture sleeve 15 and injecting the micro bubbles, after the culture sleeve 15 is installed in the culture well 14, the open 123 end of the culture sleeve 15 is contacted with the bottom of the culture dish 11, and the top of the blocking head 152 is parallel to the upper surface of the culture dish 11.
Alternatively, in the step of installing the culture dish 11, the culture holes 14 in the culture dish 11 can be in one-to-one correspondence with the connection holes 131 on the base 13 by moving the position of the culture dish 11 in the accommodating groove 122 left and right, and the bottom surface of the culture dish 11 is matched with the upper surface of the base 13, so as to ensure the coaxiality between the corresponding culture holes 14 and the connection holes 131.
The operation method of the ultrasonic microbubble cell stimulation device provided by the embodiment of the invention has the advantages of simple steps and easiness in operation, and the convenience of experiments is improved. In the specific operation process, the parallelism of the base 13, the positioning frame 12, the probe 161 and the culture dish 11 is guaranteed, the ultrasonic action field stimulated by the probe 161 can be uniformly and uniformly applied to the cells in the culture dish 11, and the contrast of the experiment is improved.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (14)
1. An ultrasonic microbubble cell stimulation device comprises a culture dish for cell culture, a positioning frame for bearing and placing the culture dish and a base for supporting the positioning frame, and is characterized in that a culture hole is formed in the culture dish, a sealing film is arranged on an opening end of the culture hole, and a culture sleeve which is used for injecting microbubbles into the culture hole and can prevent air from being mixed with the microbubbles after the culture dish is sealed and inverted by the sealing film is arranged in the culture hole; the base is connected with a conduction assembly which is used for conducting ultrasonic waves to stimulate cells and can adjust the distance between the base and the culture dish; the base is connected with the positioning frame through a positioning component;
the culture sleeve comprises a cylinder body with two open ends, one end of the cylinder body is connected with a separation head used for separating air from entering the cylinder body, the separation head is provided with an injection hole used for injecting the micro-bubbles into the cylinder body, and the other end of the cylinder body is inserted into the culture hole.
2. The ultrasound microbubble cell-stimulating device of claim 1 wherein the axial cross-section of the blocking head is "tapered" and the injection hole opens at the top of the blocking head.
3. The ultrasonic microbubble cell stimulation device according to claim 2, wherein the base is provided with a connecting hole for positioning and connecting the conducting assembly, and one end of the conducting assembly is arranged in the connecting hole.
4. The ultrasonic microbubble cell stimulation device according to claim 3, wherein a positioning step is further provided in the connecting hole.
5. The ultrasonic microbubble cell stimulation device according to claim 4, wherein the conduction assembly comprises a probe and a collimator, one end of the probe is inserted into the connecting hole and abuts against the positioning step, the collimator is sleeved on the probe and provided with a connecting structure therebetween, and one end of the collimator opposite to the culture dish is provided with a sound-transmitting membrane.
6. The ultrasonic microbubble cell stimulation device of claim 5, wherein the connection structure comprises an external thread provided on an outer wall of the probe and an internal thread correspondingly provided on an inner wall of the collimator, the external thread engaging the internal thread.
7. The apparatus according to any one of claims 1 to 6, wherein the positioning assembly comprises a guide rod disposed on the base and a guide hole disposed on the positioning frame, one end of the guide rod is fixed on the base, the other end of the guide rod movably penetrates the guide hole, and a positioning structure is disposed between the guide rod and the positioning structure for positioning and adjusting the position.
8. The ultrasonic microbubble cell stimulation device according to claim 7, wherein the positioning structure comprises a threaded hole formed in the positioning frame and a locking member for abutting against the guide rod, the threaded hole is arranged perpendicular to the axial direction of the guide hole, and the locking member is screwed in the threaded hole.
9. The ultrasonic microbubble stimulation cell device according to claim 8, wherein a plurality of culture wells are arranged in the culture dish, and a plurality of connection holes are correspondingly arranged on the base.
10. The apparatus according to claim 9, wherein the positioning frame comprises a frame body, the frame body is provided with a receiving groove for receiving and placing the culture dish and being capable of moving and positioning reciprocally, and the bottom of the receiving groove is provided with an opening for allowing the collimator to pass through and contact with the culture dish.
11. The ultrasonic microbubble cell stimulation device according to claim 10, wherein a positioning member for positioning the adjustment position of the culture dish is provided on the positioning frame.
12. An operation method of the ultrasonic microbubble cell stimulation device, which adopts the ultrasonic microbubble cell stimulation device as claimed in any one of claims 1, 7, 8 and 11, and is characterized in that the operation method comprises the following steps:
the guide rod mounting step: one end of the guide rod is fixedly connected to the base;
the conductive component connecting and assembling step: mounting the probe on the base and connecting the collimator to the probe;
the positioning frame mounting step: the other end of the guide rod penetrates through the guide hole, and the position of the positioning frame on the guide rod is locked through the locking piece;
the culture sleeve installation and the microbubble injection step: the culture sleeve is inserted into the culture hole, the microvesicles are injected into the culture sleeve through the injection hole, and finally the open end of the culture dish is sealed through the sealing membrane.
The culture dish installation step: the sealed culture dish is inverted in the accommodating groove, the placing position is adjusted, the culture holes correspond to the connecting holes one to one, and then the position of the culture dish is fixed through the positioning piece.
13. The method of claim 12, wherein in the spacer mounting step, after the spacer is coupled to the guide bar, the coupling position of the spacer is adjusted so that the bottom surface of the spacer is parallel to the upper surface of the base.
14. The method of claim 12, wherein the sheath is installed in the culture well such that the open end of the sheath is in contact with the bottom of the dish and the top of the blocking head is parallel to the upper surface of the dish after the sheath is installed in the culture well and the microbubble is injected into the culture well.
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|---|---|---|---|---|
| CN106635793A (en) * | 2017-02-07 | 2017-05-10 | 重庆科技学院 | Culture dish for ultrasonic experiment and use method |
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