CN109924253A - A kind of compound continuous sterilization device of ultrasound-heat and method for disinfection for sterilization of milk - Google Patents
A kind of compound continuous sterilization device of ultrasound-heat and method for disinfection for sterilization of milk Download PDFInfo
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- CN109924253A CN109924253A CN201910276557.0A CN201910276557A CN109924253A CN 109924253 A CN109924253 A CN 109924253A CN 201910276557 A CN201910276557 A CN 201910276557A CN 109924253 A CN109924253 A CN 109924253A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/85—Food storage or conservation, e.g. cooling or drying
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Abstract
The invention discloses a kind of compound continuous sterilization devices of ultrasound-heat and method for disinfection for sterilization of milk, and device includes: several supersonic generators；Enclosed reaction chamber, has inlet port and outlet port on reaction chamber side wall, and baffle plate is arranged along feed inlet to discharge port direction in reaction chamber, separates several processing chambers being sequentially communicated by baffle plate；The ultrasonic probe being correspondingly arranged in each processing chamber, each ultrasonic probe are connected one to one by corresponding ultrasonic transducer and the supersonic generator；The collet being coated on outside the reaction chamber, the entery and delivery port on collet connect circulating water heating switch.The present invention reaches better bactericidal effect with relatively mild processing intensity, and then utmostly keeps the original quality property of food.
Description
Technical field
The invention belongs to food engineering development fields, and in particular to a kind of sterilization of milk device and method for disinfection
Background technique
Milk is most ancient one of natural drink, is known as " white blood ", the importance to human body as one can imagine.Its
Rich in multiple nutritional components such as carbohydrate, protein, fat, vitamin, minerals, have in the diet structure of people
Irreplaceable role.Because of its nutrition abundant, milk becomes the good natural medium grown for microorganism.Newly
Fresh cow's milk content of microorganisms is smaller, but since each links such as milking operation, transport storage, processing can not accomplish sterile, all can
It causes the breeding of microorganism to pollute, or even generates pathogenic bacteria.Traditional thermal sterilization technology has been widely used in dairy industry, it
Various microorganisms can be killed, and extent of killing bacteria can control, be a kind of effective sterilization mode.For example, notification number is
The Chinese utility model patent of CN207767432U discloses a kind of sterilization of milk device, including motor, L shape support rod plus
Strengthening tendons, mounting plate, supporting leg, inlet valve, feed pipe, heating rod attachment device, control panel, total control box, shaft, stirring dress
It sets, sterilize cabinet, outlet valve, discharge nozzle, conducting wire, heating rod control device, temperature sensor, heating rod, one-way exhaust valve, guarantor
Warm layer and cabinet layer.But research shows that heat treatment can largely destroy the nutritional quality of milk.
Therefore, exploitation not only efficient sterilizing but also can not cause the novel sterilizing technology of too big damage to nutrition to cause people wide
General research, wherein ultrasonic wave receives great concern because of the characteristics such as nutritive loss minimum and environmental protection caused by it.However at present
There are biggish limitations for common ultrasound processing equipment in the market, and ultrasonic power intensity is low, and the sound field density formed
It is inhomogenous, cause to be sterilized using ultrasound wave irradiation, bactericidal effect is not thorough, and the requirement of commercial sterilization is not achieved.
Summary of the invention
The present invention is by the generating device and ultrasonic probe emitter of exploitation new type ultrasonic, and by itself and thermal sterilization
Combination is provided a kind of novel sterilization of milk device and method for disinfection, enables to be reached with relatively mild processing intensity
To better bactericidal effect, and then the original quality property of food is utmostly kept, realizes continuous flow operation, it can be with other processing
Process combines, such as pasteurization, homogenizes, packs.
A kind of compound continuous sterilization device of ultrasound-heat for sterilization of milk characterized by comprising
Several supersonic generators；
Enclosed reaction chamber, has inlet port and outlet port on reaction chamber side wall, along feed inlet to discharging in reaction chamber
Baffle plate is arranged in mouth direction, separates several processing chambers being sequentially communicated by baffle plate；
The ultrasonic probe being correspondingly arranged in each processing chamber, each ultrasonic probe pass through corresponding ultrasonic transducer
It connects one to one with the supersonic generator；
The collet being coated on outside the reaction chamber, the entery and delivery port on collet connect circulating water heating switch.
Using multiple groups, high-power ultrasound generator, separated exciting oscillating circuit structure vibrate electricity compared with auto-excitation type to the present invention side by side
Line structure increases by 10% or more in output power；Ultrasonic transmitter and processing sample are all placed in closed system, can be kept away
Exempt from the aerosol generated in ultrasonic procedure or foam is diffused into environment, reduces potential source biomolecule harm and cross contamination；Using
The good double set chamber operations of soundproof effect carry out temperature control by circulating water heating exchange system therebetween, prevent sample from overheating.
The depolymerisation of ultrasonic wave can make bacterium cluster be dispersed into individual cells, be conducive to increase bacterium and react with heat
Chance；Make bacteria cell wall and cell membrane damage by the way that heat-exchange system is heated, the various shearing forces generated in ultrasonic procedure
Impaired cell is further acted on, large stretch of cell and fragment cracked, ultrasound and heat synergistic effect, enhancing sterilization effect are caused
Fruit.
It baffling channel and is arranged in a one-to-one correspondence ultrasonic probe and realizes continuous flow operation, increase ultrasonic probe and material
Total time of contact, effectively improve germicidal efficiency.
Preferably, the baffle plate interval is fixed in reaction chamber on bottom and top plate.
It is further preferred that inlet port and outlet port are close to bottom setting, close to the baffle plate of inlet port and outlet port
It is each attached to bottom, intermediate baffle plate interval is fixed on top plate and bottom plate, is formed in reaction chamber between upwelling and downwelling
Every the baffling channel of distribution.
Preferably, multinode is distributed several ultrasonic transmitters, each ultrasonic wave on the side of the ultrasonic probe
All ultrasonic transmitters on probe are controlled by the corresponding ultrasonic transducer of the ultrasonic probe.
It is further preferred that the ultrasonic transducer is fixed on alignment processing top of chamber, with corresponding ultrasonic probe
Connection.Ultrasonic transducer is fixed on alignment processing top of chamber, convenient for safeguarding；The single transmitting ultrasonic wave in top, the sound field of generation
Density unevenness one, multinode ultrasonic transmitter its sound wave can from the multiple nodes transmittings in side, rather than only from top emission,
More uniform sound field density can be generated.
Preferably, several described supersonic generators are set on movable supporting frame in regular array, described removable
The supersonic generator mounting hole of regular array is had on stabilizer blade.Supersonic generator one-to-one correspondence is installed on corresponding mounting hole
In.Movable supporting frame bottom has idler wheel.
Supersonic generator is placed on individual support frame, facilitates operation and maintenance.The system can be with other processing streams
Journey combines, such as homogenizes, packs.
Preferably, temperature sensor is arranged in the discharge outlet；The circulating water heating switch includes:
Cold rinse bank connects into chilled water circuit into, the place mouth of a river with the collet, water supply pump and first is arranged in chilled water circuit
Solenoid valve；
Hot water storgae connects into hot-water return into, the place mouth of a river with the collet, heat-exchanger pump and second is arranged on hot-water return
Solenoid valve；
Controller, the temperature sensor access the controller, the water supply pump, the first solenoid valve, heat-exchanger pump and second
Solenoid valve accesses and is controlled by the controller.
It is further preferred that the controller is PID controller or PLC controller.
Heat-exchange system contains one-30-100 DEG C of low temperature thermostat bath, is divided into cold rinse bank and hot water storgae, and system is logical
Cross valve on and off control hot water or cold water circulation.Controller determines starting circulation according to the temperature information that temperature sensor is fed back
Hot water or cooling water system, such as set temperature are 55 DEG C, and what is first opened is circulating hot water control system, if system temperature
It is excessively high, it is then turned on recirculating cooling water system, it is ensured that temperature is controlled at 55 DEG C.
The present invention also provides a kind of methods for carrying out sterilization of milk using ultrasound-compound continuous sterilization device of heat, to milk
Middle staphylococcus aureus carries out sterilization processing, includes the following steps:
Milk to be sterilized is sent into reaction chamber by feed inlet, along the baffling channel that baffle plate is formed followed by all places
Chamber is managed, is finally discharged by discharge port；Start all supersonic generator and ultrasonic transducer, convection current while being sent into milk
The milk of reacted room carries out ultrasonic sterilization；
In sterilization process, the loop temperature-control liquid temperature in collet is adjusted by circulating water cooling device, is guaranteed in reaction chamber
The temperature of milk is 55-63 DEG C.
Preferably, ultrasonic treatment frequency be 20kHz, processing power 200-400W, processing the time be 5-20min.Into one
Preferably, processing frequency 20kHz, processing power 400W, treatment temperature are 55 DEG C to step, and the processing time is 20min,
In the above method, the milk after the hot composite bactericidal technical treatment of sound is referring to GB 4789.2-2010 food safety state
Family's standard " measurement of food microbiological examination total plate count " method carries out microbial count measurement, and to the grain diameter of milk
The situation of change of milk quality after carrying out assessment processing is measured with protein content.
Compared with prior art, the experiment proves that, apparatus of the present invention and method improve microorganism in milk
Efficiency is killed, and utmostly keeps the nutritional quality of milk, is conducive to the industry application of popularization sound heat integration sterilization technology.
Detailed description of the invention
Fig. 1 is schematic structural diagram of the device of the invention.
Fig. 2 is the connection relationship diagram of heat-exchange device and collet.
Appended drawing reference as shown in the figure is as follows:
1- supersonic generator 2- ultrasonic transducer 3- ultrasonic probe
4- continous way processing chamber 5- collet 6- heat-exchange device
7- feed inlet 8- discharge port 9- loop temperature-control liquid
601- cold rinse bank 602- hot water storgae 603- water supply pump
604- the first solenoid valve 605- heat-exchanger pump 606- second solenoid valve
Specific embodiment
As shown in Figure 1, a kind of ultrasound-compound continuous sterilization device of heat, the supersonic generator 1 being arranged side by side including multiple groups
And reactor.
Multiple groups supersonic generator 1 arranged side by side is mounted on movable supporting frame by multiple supersonic generator regular arrays
On, movable supporting frame includes rack body and the idler wheel positioned at bracket local bottom, and rule setting installs lattice on rack body, often
One supersonic generator of corresponding installation in a installation lattice.
Reactor is using double set chamber closed reactors, including enclosed reaction chamber and the collet being coated on outside reaction chamber
5, feed inlet 7 is opened up on the side wall of reaction chamber side, opens up discharge port 8 on the opposite other side, in present embodiment, feed inlet and
Discharge port is close to bottom setting, and feed inlet connects feed storage tank, discharge port connection discharging storage tank by feed pump.
Baffle plate is set in reaction chamber, and baffle plate interval is fixed in reaction chamber on bottom and top plate, by feed inlet to out
Material mouth forms baffling channel, between feed inlet side baffle plate and reaction chamber wall, between adjacent baffle and close to out
It is several processing chamber housings being sequentially communicated between material mouth side baffle plate and reaction chamber wall, upper up-flow and decline in processing chamber housing
Stream is spaced apart, and Vltrasonic device is independently arranged in each processing chamber housing, and Vltrasonic device includes that ultrasonic transducer 2 and ultrasonic wave are visited
First 3, ultrasonic transducer is installed on corresponding each processing chamber, ultrasonic probe and ultrasonic waves at the top of reaction chamber by support frame
Energy device connection, correspondence protrude into each processing chamber, and multinode distributed ultrasound transmitter (is popped one's head in vertically on ultrasonic probe
Ultrasonic transmitter is arranged in uniform intervals on side), probe extends downward at reaction chamber bottom, each ultrasonic wave transducer
Device connects one to one supersonic generator of the multiple groups side by side in supersonic generator 1, all ultrasonic waves hair on each probe
Emitter ultrasonic transducer signal connection corresponding with the probe.
Temperature sensor (not shown) is arranged in discharge outlet, detects the temperature of oral fluid body, has water inlet on collet
The connection relationship of mouth and water outlet, heat-exchange device and collet is (controller part is not shown) as shown in Figure 2, including cryogenic thermostat
Slot, pump assembly, valve member and controller part, low temperature thermostat bath include a cold rinse bank 601 and a hot water storgae 602,
Hot water storgae has heating function.Cold rinse bank 601 connects the water inlet of collet by water supply pump 603, and the water outlet of collet passes through pipe
Road is back to cold rinse bank, is arranged the first solenoid valve 604 on return line, cold rinse bank 601, water supply pump 603, collet into, place's water
Mouth and return line are formed together chilled water circuit；Hot water storgae 602 connects the water inlet of collet by heat-exchanger pump 605, collet
Water outlet is back to hot water storgae by pipeline, be arranged second solenoid valve 606 on return line, hot water storgae, heat-exchanger pump, collet
Into, place the mouth of a river and return line be formed together hot-water return.Temperature sensor access controller, water supply pump 603, the first electromagnetism
Valve 604, heat-exchanger pump 605 and second solenoid valve 606 access and are controlled by controller, and controller uses PID or PLC.
It is as follows to sterilize process:
Milk to be sterilized is sent into reaction chamber by feed inlet, along the baffling channel that baffle plate is formed followed by all places
Chamber is managed, is finally discharged by discharge port；Start all supersonic generator and ultrasonic transducer, convection current while being sent into milk
The milk of reacted room carries out ultrasonic sterilization；Ultrasonic treatment frequency be 20kHz, processing power 200-400W, processing the time be
5-20min.In sterilization process, the temperature of discharge outlet milk is monitored by temperature sensor, discharge port temperature is set as 55~
63 DEG C, temperature is regulated and controled by heat-exchange device.
To set outlet temperature as 55 DEG C, what is first opened is circulating hot water control system, when temperature reaches setting temperature
Heat circulating system is closed after degree, hot water is pumped into collet by hot water in Thermal Cycling, and water outlet is returned through second solenoid valve
Hot water storgae.If system temperature is excessively high in sterilization process, be then turned on recirculating cooling water system, during circulating chilled water, cold water by
Water supply pump is pumped into collet, and collet water outlet returns to cold rinse bank through the first solenoid valve.Cold cycling alternate run, it is ensured that temperature control
At 55 DEG C.
Combined with specific embodiments below, the present invention is further illustrated.
Embodiment 1
The hot composite bactericidal technical treatment of one sound
It is inoculated with the preparation of staphylococcus aureus milk
Staphylococcus aureus reference culture ATCC 25923 is inoculated in broth medium, 37 DEG C of shaking table cultures
To logarithmic growth phase.Using 0.85% (mass percentage) sterile saline washing logarithmic growth phase thallus twice, weight
Outstanding, making cell concentration is about 109CFU/mL, is then 108CFU/mL's according to 1:9 (bacterium solution: milk) ratio preparation inoculum concentration
Milk.
(2) the hot combined processing of sound
Experimental group: inoculation milk is handled with novel sound hot composite bactericidal device, specific steps: by 300mL milk
It is fitted into closed processes chamber, multinode ultrasonic probe gos deep into liquid level, opens cooling water circulating device, opens large power supersonic
Device, adjusting processing frequency are 20kHz, and processing power 400W, treatment temperature is 55 DEG C, and the processing time is 20min, is finally obtained
Milk after processing.
Control group: staphylococcus aureus bacteria suspension only carries out pasteurize processing (63 DEG C of heating without ultrasonic treatment
30min)。
Two detections
The measuring method of total plate count
Take 1mL treated milk to take 0.1mL to distinguish with 0.85% sterile saline gradient dilution to suitable concentration
It is added in non-selective culture TSA, 37 DEG C are cultivated 24 hours, and clump count is then recorded.
Albumen partial size detection method
Milk sample is diluted 1000 times at room temperature, 0.45m micro-filtration film process is then used, utilizes Zetasizer Nano
ZS nanometer particle size potentiometric analyzer is measured the protein-colloid partial size in sample.
Protein content determination
It is measured using bradford method.
As a result as follows: control group and experimental group can make staphylococcus aureus quantity reduce 5 logarithm ranks, control
Protein partial size is 3.4nm, protein content 2.5% after group Pasteur's processing.Albumen plasmid after the hot combined processing of experimental group sound
Diameter is 2.5nm, protein content 2.9%.
It can be seen that method of the invention can not only improve germicidal efficiency, and milk protein partial size can be effectively reduced,
It may advantageously facilitate the absorption of its nutritional ingredient, and preferably keep the quality and content of original protein in cow's milk
Embodiment 2
One heat integration ultrasonic sterilization technical treatment
Bacterial suspension preparation
With in embodiment 1 one the step of the method for (1) it is identical.
Combined Treatment
With in embodiment 1 one the step of (2) method it is essentially identical, processing power is only adjusted to 200W, processing temperature
Degree is 63 DEG C, and the processing time is 7.5min.
Two detections
It is identical as two method in embodiment 1.
As a result as follows: control group and experimental group can make staphylococcus aureus quantity reduce 5 logarithm ranks, control
Protein partial size is 3.4nm, protein content 2.5% after group Pasteur's processing.Albumen plasmid after the hot combined processing of experimental group sound
Diameter is 2.7nm, protein content 3.2%.It can be seen that method of the invention has preferably kept original protein in cow's milk
Quality and content.
The present embodiment has better effect compared with embodiment 1, to milk quality maintenance.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included within the scope of the present invention.
Claims (9)
1. a kind of compound continuous sterilization device of ultrasound-heat for sterilization of milk characterized by comprising
Several supersonic generators；
Enclosed reaction chamber, has inlet port and outlet port on reaction chamber side wall, along feed inlet to discharge port side in reaction chamber
To setting baffle plate, several processing chambers being sequentially communicated are separated by baffle plate；
The ultrasonic probe being correspondingly arranged in each processing chamber, each ultrasonic probe pass through corresponding ultrasonic transducer and institute
Supersonic generator is stated to connect one to one；
The collet being coated on outside the reaction chamber, the entery and delivery port on collet connect circulating water heating switch.
2. ultrasound-compound continuous sterilization device of heat according to claim 1, which is characterized in that the baffle plate interval is fixed
In reaction chamber on bottom and top plate.
3. ultrasound-compound continuous sterilization device of heat according to claim 1, which is characterized in that the side of the ultrasonic probe
Multinode is distributed several ultrasonic transmitters on face, and all ultrasonic transmitters on each ultrasonic probe are controlled by this
The corresponding ultrasonic transducer of ultrasonic probe.
4. ultrasound-compound continuous sterilization device of heat according to claim 3, which is characterized in that the ultrasonic transducer is solid
Due to alignment processing top of chamber, connected with corresponding ultrasonic probe.
5. ultrasound-compound continuous sterilization device of heat according to claim 1, which is characterized in that several ultrasonic waves hair
Raw device is set on movable supporting frame in regular array, and the supersonic generator on the movable support leg with regular array is pacified
Fill hole.
6. ultrasound-compound continuous sterilization device of heat according to claim 1, which is characterized in that the discharge outlet setting temperature
Spend sensor；The circulating water heating switch includes:
Cold rinse bank connects into chilled water circuit into, the place mouth of a river with the collet, water supply pump and first electromagnetism is arranged in chilled water circuit
Valve；
Hot water storgae connects into hot-water return into, the place mouth of a river with the collet, heat-exchanger pump and second electromagnetism is arranged on hot-water return
Valve；
Controller, the temperature sensor access the controller, the water supply pump, the first solenoid valve, heat-exchanger pump and the second electromagnetism
Valve accesses and is controlled by the controller.
7. ultrasound-compound continuous sterilization device of heat according to claim 6, which is characterized in that the controller is PID control
Device or PLC controller.
8. a kind of method for carrying out sterilization of milk using ultrasound as described in claim 1-compound continuous sterilization device of heat, feature
It is, includes the following steps:
Milk to be sterilized is sent into reaction chamber by feed inlet, along the baffling channel that baffle plate is formed followed by all processing
Chamber is finally discharged by discharge port；Start all supersonic generator and ultrasonic transducer while being sent into milk, to flowing through
The milk of reaction chamber carries out ultrasonic sterilization；
In sterilization process, the loop temperature-control liquid temperature in collet is adjusted by circulating water cooling device, guarantees milk in reaction chamber
Temperature be 55-63 DEG C.
9. method according to claim 8, which is characterized in that ultrasonic treatment frequency is 15~25kHz, processing power 200-
400W, processing time are 5-20min.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910276557.0A CN109924253B (en) | 2019-04-08 | 2019-04-08 | Ultrasonic-thermal composite continuous sterilization device and sterilization method for milk sterilization |
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| CN201910276557.0A CN109924253B (en) | 2019-04-08 | 2019-04-08 | Ultrasonic-thermal composite continuous sterilization device and sterilization method for milk sterilization |
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| CN109924253B CN109924253B (en) | 2023-06-27 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111317029A (en) * | 2020-04-09 | 2020-06-23 | 大连九羊乳业股份有限公司 | High-efficiency sterilization method combining pasteurization and irradiation sterilization of goat milk |
| CN114027357A (en) * | 2021-12-09 | 2022-02-11 | 齐鲁工业大学 | Functional dairy product containing whey oligopeptide and preparation method thereof |
| CN114223711A (en) * | 2021-12-22 | 2022-03-25 | 华中农业大学 | Ultrasonic micro-heating synergistic sterilization device and method for liquid eggs |
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|---|---|---|---|---|
| CN101856136A (en) * | 2010-05-26 | 2010-10-13 | 华南理工大学 | Ultrasonic continuous sterilization device and method thereof |
| CN104815596A (en) * | 2015-05-21 | 2015-08-05 | 杨浩 | High-efficiency continuous ultrasonic cell wall-breaking treating device |
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2019
- 2019-04-08 CN CN201910276557.0A patent/CN109924253B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101856136A (en) * | 2010-05-26 | 2010-10-13 | 华南理工大学 | Ultrasonic continuous sterilization device and method thereof |
| CN104815596A (en) * | 2015-05-21 | 2015-08-05 | 杨浩 | High-efficiency continuous ultrasonic cell wall-breaking treating device |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111317029A (en) * | 2020-04-09 | 2020-06-23 | 大连九羊乳业股份有限公司 | High-efficiency sterilization method combining pasteurization and irradiation sterilization of goat milk |
| CN114027357A (en) * | 2021-12-09 | 2022-02-11 | 齐鲁工业大学 | Functional dairy product containing whey oligopeptide and preparation method thereof |
| CN114223711A (en) * | 2021-12-22 | 2022-03-25 | 华中农业大学 | Ultrasonic micro-heating synergistic sterilization device and method for liquid eggs |
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