CN108740684A - Press the compound inactivation brood cell's method and apparatus of thermoacoustic - Google Patents
Press the compound inactivation brood cell's method and apparatus of thermoacoustic Download PDFInfo
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- CN108740684A CN108740684A CN201810421105.2A CN201810421105A CN108740684A CN 108740684 A CN108740684 A CN 108740684A CN 201810421105 A CN201810421105 A CN 201810421105A CN 108740684 A CN108740684 A CN 108740684A
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- pressure
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- thermoacoustic
- brood cell
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- 244000144987 brood Species 0.000 title claims abstract description 64
- 238000000034 method Methods 0.000 title claims abstract description 48
- 230000002779 inactivation Effects 0.000 title claims abstract description 45
- 150000001875 compounds Chemical class 0.000 title claims abstract description 33
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 81
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 73
- 239000000523 sample Substances 0.000 claims abstract description 46
- 230000001954 sterilising effect Effects 0.000 claims abstract description 46
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 27
- 238000001816 cooling Methods 0.000 claims abstract description 19
- 230000008569 process Effects 0.000 claims abstract description 15
- 229910001873 dinitrogen Inorganic materials 0.000 claims abstract description 13
- 238000009434 installation Methods 0.000 claims abstract description 6
- 238000010438 heat treatment Methods 0.000 claims description 24
- 239000007789 gas Substances 0.000 claims description 11
- 229910052757 nitrogen Inorganic materials 0.000 claims description 7
- 239000000498 cooling water Substances 0.000 claims description 5
- 239000008236 heating water Substances 0.000 claims description 3
- 230000005611 electricity Effects 0.000 claims description 2
- 238000000465 moulding Methods 0.000 claims 1
- 235000013305 food Nutrition 0.000 abstract description 16
- 230000000694 effects Effects 0.000 abstract description 15
- 238000012545 processing Methods 0.000 abstract description 13
- 238000002604 ultrasonography Methods 0.000 abstract description 8
- 241000726221 Gemma Species 0.000 abstract description 4
- 238000005265 energy consumption Methods 0.000 abstract description 2
- 230000010354 integration Effects 0.000 abstract description 2
- 239000006227 byproduct Substances 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 49
- 230000000052 comparative effect Effects 0.000 description 17
- 241000894006 Bacteria Species 0.000 description 7
- 238000010586 diagram Methods 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 102000004190 Enzymes Human genes 0.000 description 6
- 108090000790 Enzymes Proteins 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 6
- 230000006872 improvement Effects 0.000 description 6
- 239000000725 suspension Substances 0.000 description 6
- 230000000844 anti-bacterial effect Effects 0.000 description 5
- 239000008223 sterile water Substances 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- VZSRBBMJRBPUNF-UHFFFAOYSA-N 2-(2,3-dihydro-1H-inden-2-ylamino)-N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]pyrimidine-5-carboxamide Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)C(=O)NCCC(N1CC2=C(CC1)NN=N2)=O VZSRBBMJRBPUNF-UHFFFAOYSA-N 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000000796 flavoring agent Substances 0.000 description 3
- 235000019634 flavors Nutrition 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- YLZOPXRUQYQQID-UHFFFAOYSA-N 3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)-1-[4-[2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidin-5-yl]piperazin-1-yl]propan-1-one Chemical compound N1N=NC=2CN(CCC=21)CCC(=O)N1CCN(CC1)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F YLZOPXRUQYQQID-UHFFFAOYSA-N 0.000 description 2
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- MKYBYDHXWVHEJW-UHFFFAOYSA-N N-[1-oxo-1-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propan-2-yl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(C(C)NC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 MKYBYDHXWVHEJW-UHFFFAOYSA-N 0.000 description 2
- NIPNSKYNPDTRPC-UHFFFAOYSA-N N-[2-oxo-2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 NIPNSKYNPDTRPC-UHFFFAOYSA-N 0.000 description 2
- AFCARXCZXQIEQB-UHFFFAOYSA-N N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CCNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 AFCARXCZXQIEQB-UHFFFAOYSA-N 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- JNLCUVJKTXKKSG-UHFFFAOYSA-N 2-hydroxypropane-1,2,3-tricarboxylic acid;lead Chemical compound [Pb].OC(=O)CC(O)(C(O)=O)CC(O)=O JNLCUVJKTXKKSG-UHFFFAOYSA-N 0.000 description 1
- 241000193755 Bacillus cereus Species 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- ABUBSBSOTTXVPV-UHFFFAOYSA-H [U+6].CC([O-])=O.CC([O-])=O.CC([O-])=O.CC([O-])=O.CC([O-])=O.CC([O-])=O Chemical compound [U+6].CC([O-])=O.CC([O-])=O.CC([O-])=O.CC([O-])=O.CC([O-])=O.CC([O-])=O ABUBSBSOTTXVPV-UHFFFAOYSA-H 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 235000013405 beer Nutrition 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 239000012267 brine Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 230000009849 deactivation Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 230000009246 food effect Effects 0.000 description 1
- 238000009920 food preservation Methods 0.000 description 1
- 235000021393 food security Nutrition 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 235000021056 liquid food Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 235000015205 orange juice Nutrition 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000012047 saturated solution Substances 0.000 description 1
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 description 1
- 235000013555 soy sauce Nutrition 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/015—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with pressure variation, shock, acceleration or shear stress or cavitation
- A23L3/0155—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with pressure variation, shock, acceleration or shear stress or cavitation using sub- or super-atmospheric pressures, or pressure variations transmitted by a liquid or gas
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/02—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by heating materials in packages which are progressively transported, continuously or stepwise, through the apparatus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/10—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by heating materials in packages which are not progressively transported through the apparatus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/16—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by heating loose unpacked materials
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/26—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by irradiation without heating
- A23L3/30—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by irradiation without heating by treatment with ultrasonic waves
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/85—Food storage or conservation, e.g. cooling or drying
Abstract
The invention discloses a kind of compound inactivation brood cell's methods of pressure thermoacoustic, and by product subject to sterilization in 75~85 DEG C, 450~550kPa of pressure of temperature, amplitude 100%, frequency is that 20khz carries out 25~35min of supersound process, and gemma Inactivation Effect is notable.The present invention handles processing subject to sterilization using the compound method of pressure thermoacoustic, there is higher brood cell to inactivate efficiency under conditions of temperature is no more than 85 DEG C can not only reduce energy consumption compared with prior art, the influence to food quality can also be reduced, is ensured food safety.Invention additionally discloses a kind of compound inactivation brood cell's equipment of pressure thermoacoustic, including disinfection tank, nitrogen gas tank, water cooling pond, hot-tub, temperature relay, ultrasonic vibration installation, temp probe, pump group part and electromagnetic valve component, meet the equipment requirement that above method ultrasound, pressure and heat integration are sterilized.
Description
Technical field
The present invention relates to method for disinfection and equipment, and in particular to a kind of compound inactivation brood cell's method and apparatus of pressure thermoacoustic.
Background technology
With economic development and improvement of living standards, food becomes a more and more active stage, consumer couple
The food oneself eaten has a higher requirement, and some the important attributes for influencing food such as flavor, quality, appearance and nutrition
There is inextricable relationship Deng with processing method.Heat treatment is the most traditional sterilization mode of most original, and is industrially answered at present
With widest, there are many researchs to be dedicated to thermally-sterilized mechanism and the influence to food quality.In recent years, in order to meet
The demand of personalized food, all kinds of novel foodstuff processing technologies and method are come into being, but due to various reasons also not in work
It is popularized in industry, therefore so far, thermal sterilization is still the main and most efficient method of food sterilization.
Ultrasonic wave is a kind of effective assisted sterilization method, has been used successfully to the fields such as wastewater treatment, drinking water disinfection,
Application in liquid food sterilizing also has more research, such as beer, orange juice, soy sauce.Sterilization effect possessed by ultrasonic wave
Power reaches the rate for accelerating food sterilizing mainly due to cavitation caused by ultrasonic wave.But individual ul-trasonic irradiation
The effect being extremely difficult to required by practical application.
Under condition of high voltage, certain change all occurs for the inhereditary material of microorganism, protein structure etc., cell wall and thin
Some or all of forfeiture is even resulted in microbial inactivation by after birth by a degree of damage, the physiological function of microorganism
It is even dead, but high pressure technique is single use with certain limitation, all bacteriums cannot be killed completely, and high pressure is killed
Bacterium becomes apparent in conjunction with other methods bactericidal effect.
Non-thermal sterilization maintains the flavor and nutriment of product to the full extent while effectively killing microorganism, keeps away
Exempt from thermal sterilization and destroys the original structure of food and flavor.But non-thermal sterilization has certain limitation, independent role is often
Effect is poor, and therefore, can combine heat with every non-thermal sterilization technology improves self shortcoming, so that non-thermal technologies is had and preferably answers
Use foreground.
Hypopus of the brood cell as production brood cell bacterium, when growing environment lacks nutrition or the accumulation of detrimental metabolic substance is excessive
It is formed.Brood cell is the entire strongest life entity of living nature resistance, in heat resistanceheat resistant, anti-chemicals, radioresistance, anti-high pressure etc.
It is even more leading.The extremely strong resistance of brood cell is a huge challenge to sterilizing link in food processing, if cannot effectively kill
It goes out or controls brood cell, food spoilage, the toxin of generation can be caused also to constitute potential threat to health after germination.Existing work
Common sterilization means are heat treatment in industry, and the heat resistance of brood cell is high, such as D value of the Bacillus subtillis brood cell at 88 DEG C
For 30.2min, therefore to ensure food security, it has to excessively heat treatment.Therefore, a kind of effective brood cell's inactivation means tool is found
It is of great importance.
It presses thermoacoustic as a kind of New Processing, is chiefly used in improving effect of food preservation, such as non-patent literature《Press thermoacoustic
Handle the commentary influenced on enzymatic activity》Inform that manothermosonication can make enzyme inactivation reach fresh-keeping effect.Enzyme is as a kind of common
Protein, some simple processing can be such that its activity reduces even to inactivate, such as individually heat treatment or supersound process,
And special hypopus structure of the brood cell as bacterium, can not processing work corresponding with reference enzyme progress, and not yet inquire pressure
Thermo-acoustic technology is applied to the inactivation pertinent literature of brood cell.
Invention content
The technical problem to be solved by the present invention is to propose a kind of compound inactivation brood cell's method and apparatus of pressure thermoacoustic.
To solve the above-mentioned problems, the present invention proposes a kind of compound inactivation brood cell's equipment of pressure thermoacoustic：
The compound inactivation brood cell's equipment of pressure thermoacoustic includes disinfection tank, chuck, nitrogen gas tank, water cooling pond, hot-tub and ultrasound
Vibrating device；
The ultrasonic vibration installation includes the ultrasonic generator, ultrasonic transducer and ultrasonic probe being sequentially connected；
The disinfection tank is connected with nitrogen gas tank；
The chuck is sleeved on disinfection tank outer surface, and chuck is connected with water cooling pond and hot-tub respectively；
The ultrasonic transducer is mounted at the top of disinfection tank, and ultrasonic probe is mounted on ultrasonic transducer, and ultrasonic probe
End part seal insert into the inner in chamber I after the disinfection tank.
Improvement as the compound inactivation brood cell's equipment of present invention pressure thermoacoustic：
The compound inactivation brood cell's equipment of pressure thermoacoustic further includes that pump group part, electromagnetic valve component, temperature relay and temperature are visited
Head；
The temperature relay is connected with pump group part, electromagnetic valve component and temp probe respectively；
The end of the temp probe is stretched into inner cavity I.
As being further improved for the present invention pressure compound inactivation brood cell's equipment of thermoacoustic：
The pump group part includes water supply pump and heat-exchanger pump；Electromagnetic valve component includes solenoid valve I and solenoid valve II；
The water supply pump, heat-exchanger pump, solenoid valve I and solenoid valve II are connected with temperature relay respectively.
It is equipped with water outlet and water inlet on the chuck successively from top to bottom；
The water inlet is connected by water supply pump with water cooling pond, is also connected with hot-tub by heat-exchanger pump；
The water outlet is connected by solenoid valve I with water cooling pond, is also connected with hot-tub by solenoid valve II；
As being further improved for the present invention pressure compound inactivation brood cell's equipment of thermoacoustic：
The disinfection tank is equipped with air inlet and gas outlet；
Pressure reducing valve is equipped at the air inlet, air inlet is connected by pressure reducing valve with nitrogen gas tank；
Relief valve is equipped at the gas outlet；
The chuck is below air inlet and gas outlet.
To solve the above-mentioned problems, the present invention also proposes a kind of compound inactivation brood cell method of pressure thermoacoustic (this method is using upper
State equipment realization), include the following steps：
Product subject to sterilization is put into the inner cavity I of disinfection tank, in 75~85 DEG C of heating temperature, 450~550kPa's adds
Pressure pressure, is 100% (114 μm) into row amplitude, and frequency is 25~35min of supersound process of 20khz.
Improvement as the compound inactivation brood cell's method of present invention pressure thermoacoustic：
Product subject to sterilization is put into the inner cavity I of disinfection tank, in 80 DEG C of heating temperature of temperature, in the pressurization pressure of 500kPa
Power, is 100% (114 μm) into row amplitude, and frequency is the supersound process 30min of 20khz.
The compound inactivation brood cell's further improvements in methods of thermoacoustic are pressed as the present invention：
The method of heating is：
Heating temperature is set by temperature relay first；
Temp probe detects the temperature of product subject to sterilization in real time, and temperature signal is sent to temperature relay, temperature after
Electric appliance works according to the temperature signal control electromagnetic valve component and pump group part that are received；
When the temperature of product subject to sterilization is less than set heating temperature, temperature relay controls hot water pump work, hot-tub
In (85~100 DEG C) of heating water enter in the inner cavity II of chuck；Meanwhile temperature relay control solenoid valve II is opened, chuck
Inner cavity II in water returned in hot-tub by solenoid valve II；
Conversely, then temperature relay controls cold water pump work, the cooling water (normal-temperature water, 15~35 DEG C) in water cooling pond enters
In chuck inner cavity II；Meanwhile temperature relay control solenoid valve I is opened, the water in chuck inner cavity II returns to cold water by solenoid valve I
Chi Zhong.
The compound inactivation brood cell's further improvements in methods of thermoacoustic are pressed as the present invention：
The method of the supersound process is：
Ultrasonic signal is sent out using ultrasonic generator, which is converted to electric signal and transmitted by ultrasonic transducer
Onto ultrasonic probe, the vibration of particular amplitude occurs in the inner cavity of disinfection tank I according to the signal for ultrasonic probe；
I 1 ± 0.05cm of bottom of inner cavity of the threshold value disinfection tank of the ultrasonic probe.
The compound inactivation brood cell's further improvements in methods of thermoacoustic are pressed as the present invention：
The method of pressurization is：
High-purity nitrogen is injected into the inner cavity I of disinfection tank using nitrogen gas tank, and by depressurizing pressure regulating valve, until killing
Relief valve is opened after the completion of bacterium releases nitrogen.
Compared with prior art, the technical advantages of the present invention are that：
The present invention inactivates brood cell using ultrasonic wave and method associated with high pressure and heat；At individual heat treatment, ultrasound
Reason is compared, and pressure thermoacoustic can largely inactivate brood cell.On the other hand, energy loss can be reduced by reducing sterilizing time, be risen
Energy saving, consumption reduction, environmentally friendly important function are arrived.
Description of the drawings
The specific implementation mode of the present invention is described in further detail below in conjunction with the accompanying drawings.
Fig. 1 is the present invention pressure compound inactivation brood cell's device structure schematic diagram of thermoacoustic；
Fig. 2 is brood cell's ultra microstructure schematic diagram in blank control of the present invention；
Fig. 3 is brood cell's ultra microstructure schematic diagram in the embodiment of the present invention 1；
Fig. 4 is brood cell's ultra microstructure schematic diagram in comparative example 1-1 of the present invention；
Fig. 5 is brood cell's ultra microstructure schematic diagram in comparative example 1-2 of the present invention；
Fig. 6 is brood cell's ultra microstructure schematic diagram in comparative example 1-3 of the present invention；
Fig. 7 is brood cell's ultra microstructure schematic diagram in comparative example 1-4 of the present invention.
Specific implementation mode
With reference to specific embodiment, the present invention is described further, but protection scope of the present invention is not limited in
This.
Embodiment 1, pressure the compound inactivation brood cell's equipment of thermoacoustic, as shown in Figure 1, include disinfection tank 1, nitrogen gas tank 3, water cooling pond 4,
Hot-tub 5, temperature relay 6, ultrasonic vibration installation, temp probe 8, pump group part and electromagnetic valve component.
Pump group part includes water supply pump 41 and heat-exchanger pump 51；Electromagnetic valve component includes solenoid valve I 42 and solenoid valve II 52.
Ultrasonic vibration installation includes the ultrasonic generator 72, ultrasonic transducer 70 and ultrasonic probe 71 being sequentially connected；Ultrasound
Generator 72 is connected as driving power with ultrasonic transducer 70 and ultrasonic probe 71.When ultrasonic generator 72 sends out ultrasonic signal
When, which is converted to electric signal and is transmitted on ultrasonic probe 71 by ultrasonic transducer 70, and ultrasonic probe 71 occurs specific
The vibration of amplitude.
The inner cavity I 100 of disinfection tank 1 is for placing product subject to sterilization；Ultrasonic transducer 70 is mounted on 1 top of disinfection tank,
Ultrasonic probe 71 is mounted on ultrasonic transducer 70, and the end part seal of ultrasonic probe 71 inserts into the inner after the disinfection tank 1
In chamber I 100, in the present embodiment, I 100 bottom of the inner cavity about 1cm of the threshold value disinfection tank 1 of ultrasonic probe 71.
Disinfection tank 1 is equipped with air inlet 11 and gas outlet 12, and air inlet 11 is connected by pressure reducing valve 31 with nitrogen gas tank 3,
Relief valve 32 is equipped at gas outlet 12, gas outlet 12 is connected by relief valve 32 with external environment.
In actual use, staff injects high-purity by opening nitrogen gas tank 3 into 1 inner cavity I 100 of disinfection tank
Nitrogen, and required pressure is adjusted to by pressure reducing valve 31, the releasing nitrogen of relief valve 32 is opened after the completion of sterilization (to be gone out in such as Fig. 1
At gas port 12 shown in arrow, nitrogen is discharged).
Chuck 2 is additionally provided on disinfection tank 1, chuck 2 is sleeved on 1 outer surface of disinfection tank, and is located at air inlet 11 and gas outlet
12 lower sections.It is equipped with water outlet 22 and water inlet 21 on chuck 2 successively from top to bottom；Such as heavy line in Fig. 1 and the arrow on heavy line
Shown in head, water inlet 21 is connected by water supply pump 41 with water cooling pond 4, is also connected with hot-tub 5 by heat-exchanger pump 51, that is,
Water cooling pond 4 passes sequentially through water supply pump 41 and water inlet 21 and is connected with 2 inner cavity II 101 of chuck, and hot-tub 5 passes sequentially through heat-exchanger pump
51 and water inlet 21 be connected with 2 inner cavity II 101 of chuck.Water outlet 22 is connected by solenoid valve I 42 with water cooling pond 4, also logical
Solenoid valve II 52 is crossed with hot-tub 5 to be connected.
The end of temp probe 8 is stretched into the inner cavity I 100 of disinfection tank 1, is immersed in sample subject to sterilization, and detection in real time waits for
The temperature of sterilizing sample.Temp probe 8 is connected with 6 signal of temperature relay, temperature relay 6 also with water supply pump 41, heat-exchanger pump
51, solenoid valve I 42 and solenoid valve II 52 are electrically connected (clean and tidy for drawing, therefore temperature relay 6 and heat are omitted in Fig. 1
II 52 line of water pump 51 and solenoid valve).
In practical work process, when temp probe detects sample temperature higher than setting temperature, temperature relay 6 is sent out
Go out signal and open water supply pump 41 (opening simultaneously solenoid valve I 42), cooling water enters 2 inner cavity of chuck, II 101 (2 inner cavity II 101 of chuck
In water returned in water cooling pond 4 by solenoid valve I 42)；When sample temperature is less than setting temperature, temperature relay 6 opens hot water
51 (opening simultaneously solenoid valve II 52) are pumped, heating water enters 2 inner cavity II 101 of chuck, and (water in 2 inner cavity II 101 of chuck is by electromagnetism
Valve II 52 returns in hot-tub 5).
Note：Water is heated in the present embodiment uses 95 DEG C of hot water, in actual operation, it can select 85 according to heating temperature~
100 DEG C of hot water.Cooling water uses normal-temperature water (25 DEG C) in the present embodiment.
Brood cell is handled using above-mentioned pressure thermoacoustic compound inactivation brood cell's equipment, specific processing step is as follows：
The brood cell that the present invention uses is the ATCC14579 Bacillus cereus of the Qingdao bio tech ltd Hai Bo.
1, heat：It is put into sample subject to sterilization into the inner cavity I 100 of disinfection tank 1.
25mL sterile waters are put into the inner cavity I 100 of disinfection tank 1 in the present embodiment, setting preheating temperature is 80 DEG C.
Temperature relay 6 opens heat-exchanger pump 51 (opening simultaneously solenoid valve II 52) at this time, and the hot water in hot-tub 5 passes through heat
Water pump 51 is heated into 2 inner cavity II 101 of chuck, and (water in 2 inner cavity II 101 of chuck returns to hot water by solenoid valve II 52
In pond 5).
Temp probe 8 in 1 inner cavity I 100 of disinfection tank can detect the temperature of sample subject to sterilization, temperature relay 6 in real time
Corresponding electromagnetic valve component and pump group part can be opened in real time when temperature is higher or lower than set temperature.Therefore entire sterilized
In journey the temperature of 1 inner cavity I 100 of disinfection tank be held in 80 ± 2 DEG C (signal was transmitted with water-bath there is still a need for the regular hour, because
This temperature still has certain fluctuation).
Note：In practical work process, product subject to sterilization can be directly placed into 1 inner cavity I 100 of disinfection tank and directly carry out heating killing
Bacterium.Spore suspension is directly heated to 80 DEG C in the present embodiment, also has deactivation to brood cell during heating, it is more difficult to be quantified.Cause
This is using the method preheated, you can its effect of more accurate quantityization, consequently facilitating shadow of the analysis different parameters to gemma Inactivation Effect
It rings.
2, it pressurizes and is ultrasonically treated：It is added when preheating temperature reaches 80 DEG C into sterile water and is added 2.0 × 108CFU/mL
Spore suspension 5mL.Disinfection tank 1 is closed, nitrogen gas tank 3 is opened and the pressure in the inner cavity I 100 of disinfection tank 1 is increased into 500kPa
Amplitude 100% (amplitude is 114 μm in the present embodiment) is used in combination in (atmospheric pressure 100kPa, additional to increase 400kPa pressure) pressure,
The supersound process 30min of frequency 20khz.
Note：Above-mentioned heating, ultrasound occur simultaneously with pressurized operation.
The bigger effect of pressure is better, but in real process, the higher requirement to container (disinfection tank 1) of pressure is also got over
Height when pressure is 450~550kPa, that is, meets the requirement of generic container, and have preferable bactericidal effect.
The higher bactericidal effect of temperature is better, but excessively high temperature is larger to sample subject to sterilization and apparatus damage degree,
When sterilising temp is 75~85 DEG C, i.e., sample quality subject to sterilization will not be affected greatly, and there is preferable bactericidal effect,
Compared with prior art, energy loss is also reduced.
In supersound process and non-temporal longer sterilization effect is better, considers three kinds of temperature, pressure and ultrasound processing
Mode is found in 75~85 DEG C, 450~550kPa of pressure of temperature, is 100% (114 μm) into row amplitude, and frequency is 20khz's
It is notable to be ultrasonically treated 25~35min bactericidal effects.Realize that gemma inactivation first compares with existing excessive heat treatment, the present invention is without increasing
Sterilization temperature extends sterilizing time the inactivation to gemma can be realized, and can ensure food safety and can guarantee food quality,
It can also make that sterilization temperature is low, the time is short, reduce energy consumption.
3, after the completion of step 2, stop being ultrasonically treated, and open 32 disinfection tank of relief valve, 1 inner cavity I 100 and restore normal pressure
(100kPa).Temperature is set as room temperature (25 DEG C) at this time, temperature relay transmits signal opening water supply pump 41 and (opens simultaneously electricity
42), cooling water enters 2 inner cavity II 101 of chuck, and (water in 2 inner cavity II 101 of chuck returns to water cooling pond 4 to magnet valve I by solenoid valve I 42
In), carry out cooling treatment.Wait for that temperature is cooled to room temperature (25 DEG C) taking-ups.
Comparative example 1-1, the pressure of step 2 in embodiment 1 is changed to 100kPa (normal pressure), that is, it is multiple only to carry out heating ultrasound
Close sterilization；Remaining is equal to embodiment 1.
Preheating temperature is changed to 25 DEG C (room temperatures) in comparative example 1-2,1 step 1 of embodiment, that is, only carries out pressurization ULTRASONIC COMPLEX
Sterilization；Remaining is equal to embodiment 1.
Comparative example 1-3,25mL sterile water is added 2.0 × 10 after being preheated to 80 DEG C8The spore suspension 5mL of CFU/mL, mixing
80 DEG C of processing 30min afterwards, that is, only carry out heating sterilization.
Note：Carrying out above-mentioned heat treatment can be such that enzyme inactivates.
Comparative example 1-4,25mL sterile water is preheated to 25 DEG C and is added 2.0 × 108The spore suspension 5mL of CFU/mL, uses amplitude
The supersound process 30min of 100%, frequency 20khz, that is, only carry out ultrasonic sterilization.
Note：The amount of activated inactivation of enzyme can be made by carrying out above-mentioned supersound process.
Comparative example 2-1, treatment temperature in 1 step 1 of embodiment and 2 is changed to 60 DEG C, remaining is equal to embodiment 1.
Comparative example 2-2, ultrasonic duration in 1 step 2 of embodiment is changed to 10min, remaining is equal to embodiment 1.
Experiment 1, brood cell's inactivation measure：
The solution of gained takes 0.1mL even spreads to be trained with 20mLNA+01% soluble starches after above-described embodiment 1 is sterilized
Base is supported, 37 DEG C of constant temperature incubations count after for 24 hours, and blank control are arranged (25mL sterile water mixing 5mL spore suspensions are as blank
Control) identical experiment is carried out, specific data are as shown in table 1.
The step of solution obtained by above-mentioned all comparative examples is tested in accordance with the above-mentioned embodiment 1 is tested successively, specifically
Data are as shown in table 1.
1 different disposal rear plate technology clump count unit of table：log(CFU/mL)
| Clump count/log (CFU/mL) | |
| Blank control | 7.30±0.02 |
| Embodiment 1 | 4.18±0.03 |
| Comparative example 1-1 | 6.78±0.08 |
| Comparative example 1-2 | 7.00±0.02 |
| Comparative example 1-3 | 6.92±0.12 |
| Comparative example 1-4 | 7.18±0.03 |
| Comparative example 2-1 | 6.25±0.08 |
| Comparative example 2-2 | 5.71±0.01 |
By the data of table 1 as it can be seen that can lead to that enzyme inactivates individual be ultrasonically treated, be heat-treated to the Inactivation Effect of brood cell not
Good, the effect that the processing of ultrasonic in combination pressure inactivates brood cell is not also notable, though ultrasonic in combination heat treatment has brood cell's Inactivation Effect
Collaboration but effect still not significantly, but ultrasound with have to brood cell's inactivation after pressure and heat integration effect and preferably cooperate with work
With the quantity for inactivating brood cell after processing greatly increases.
Experiment 2：Brood cell's ultra microstructure measures：
Processing group and control sample (25mL sterile water mixing 5mL spore suspensions are as blank control) solution are existed
8000r/min centrifuges 10min, and precipitation, gained is taken to precipitate with 0.85% brine 3 times (centrifugation is same as above).Secondly it uses
2.5% 4 DEG C of fixations of glutaraldehyde solution overnight, outwell fixer later, are floated with the phosphate buffer of 0.1mol/L, pH7.0
Wash sample three times, each 15min；After fixing sample 1-2h with 1% osmic acid solution again, with the phosphate of 0.1mol/L, pH7.0
Buffer solution rinses sample three times, each 15min；Gradient concentration (including 30%, 50%, 70%, 80%, 90%, 95% is used later
Six kinds of concentration) ethanol solution sample is carried out dehydrating, each concentration handles 15min, then with 100% alcohol treatment
20min is transitioned into pure acetone processing 20min later；Sample embedding is got up with embedding medium, 70 DEG C are heated overnight to get to packet
The sample buried.Sample is sliced in LEICA EMUC7 type ultramicrotome, obtains the slice of 70-90nm, is sliced through citric acid
Lead solution and 50% alcohol saturated solution of uranium acetate respectively dye 5-10min, you can it is observed in JEM-1230 transmission electron microscopes,
To compare the variation of brood cell's ultra microstructure after different disposal.
Shown in Fig. 2~7, brood cell's ultra microstructure can be destroyed by being ultrasonically treated mouth, but after manothermosonication of the present invention
Its content and morphological disruption are become apparent from, brood cell's content leakage as shown in Figure 3.
Compared with prior art, the present invention handles product subject to sterilization using the compound method of pressure thermoacoustic, can greatly improve
The inactivation efficiency of brood cell, reduces the influence to food quality, ensures food safety compared with prior art.The present invention is without improving
Sterilization temperature or extension sterilizing time can effectively kill bacteria, and to reduce cost, reduce the consumption of the energy.
Finally, it should also be noted that it is listed above be only the present invention several specific embodiments.Obviously, this hair
Bright to be not limited to above example, acceptable there are many deformations.Those skilled in the art can be from present disclosure
All deformations for directly exporting or associating, are considered as protection scope of the present invention.
Claims (9)
1. pressing the compound inactivation brood cell's equipment of thermoacoustic, it is characterised in that：
The compound inactivation brood cell's equipment of pressure thermoacoustic includes disinfection tank (1), chuck (2), nitrogen gas tank (3), water cooling pond (4), hot water
Pond (5) and ultrasonic vibration installation；
The ultrasonic vibration installation includes the ultrasonic generator (72), ultrasonic transducer (70) and ultrasonic probe being sequentially connected
(71)；
The disinfection tank (1) is connected with nitrogen gas tank (3)；
The chuck (2) is sleeved on disinfection tank (1) outer surface, and chuck (2) is connected with water cooling pond (4) and hot-tub (5) respectively
It is logical；
The ultrasonic transducer (70) is mounted at the top of disinfection tank (1), and ultrasonic probe (71) is mounted on ultrasonic transducer (70),
And the end part seal on the ultrasonic transducer inserts into the inner in chamber I (100) after disinfection tank (1).
2. the compound inactivation brood cell's equipment of pressure thermoacoustic according to claim 1, it is characterised in that：
The compound inactivation brood cell's equipment of pressure thermoacoustic further includes pump group part, electromagnetic valve component, temperature relay (6) and temp probe
(8)；
The temperature relay (6) is connected with pump group part, electromagnetic valve component and temp probe (8) respectively；
The end of the temp probe (8) is stretched into inner cavity I (100).
3. the compound inactivation brood cell's equipment of pressure thermoacoustic according to claim 2, it is characterised in that：
The pump group part includes water supply pump (41) and heat-exchanger pump (51)；Electromagnetic valve component includes solenoid valve I (42) and solenoid valve II
(52)；
The water supply pump (41), heat-exchanger pump (51), solenoid valve I (42) and solenoid valve II (52) respectively with temperature relay (6) phase
Even.
It is equipped with water outlet (22) and water inlet (21) on the chuck (2) successively from top to bottom；
The water inlet (21) is connected by water supply pump (41) with water cooling pond (4), and heat-exchanger pump (51) and hot-tub (5) are also passed through
It is connected；
The water outlet (22) is connected by solenoid valve I (42) with water cooling pond (4), and solenoid valve II (52) and hot-tub are also passed through
(5) it is connected.
4. according to any compound inactivation brood cell's equipment of pressure thermoacoustic of claim 1-3, it is characterised in that：
The disinfection tank (1) is equipped with air inlet (11) and gas outlet (12)；
Pressure reducing valve (31) is equipped at the air inlet (11), air inlet (11) is connected by pressure reducing valve (31) with nitrogen gas tank (3)；
Relief valve (32) is equipped at the gas outlet (12)；
The chuck (2) is below air inlet (11) and gas outlet (12).
5. pressing the compound inactivation brood cell's method of thermoacoustic, it is characterised in that following steps：
Product subject to sterilization is put into the inner cavity I (100) of disinfection tank (1), in 75~85 DEG C of heating temperature, 450~550kPa
Moulding pressure, into row amplitude be 100%, frequency be 20khz 25~35min of supersound process.
6. the compound inactivation brood cell's method of pressure thermoacoustic according to claim 5, it is characterised in that：
Product subject to sterilization is put into the inner cavity I (100) of disinfection tank (1), in 80 DEG C of heating temperature of temperature, in adding for 500kPa
Pressure pressure is 100% into row amplitude, and frequency is the supersound process 30min of 20khz.
7. the compound inactivation brood cell's method of pressure thermoacoustic according to claim 5 or 6, it is characterised in that：
The method of heating is：
Heating temperature is set by temperature relay (6) first；
Temp probe (8) detects the temperature of product subject to sterilization in real time, and temperature signal is sent to temperature relay (6), temperature
Relay (6) works according to the temperature signal control electromagnetic valve component and pump group part that are received；
When the temperature of product subject to sterilization is less than set heating temperature, temperature relay (6) controls heat-exchanger pump (51) and works, hot water
Heating water in pond (5) enters in the inner cavity II (101) of chuck (2)；Meanwhile temperature relay (6) control solenoid valve II (52)
It opens, the water in the inner cavity II (101) of chuck (2) is returned to by solenoid valve II (52) in hot-tub (5)；
Conversely, then temperature relay (6) control water supply pump (41) works, the cooling water in water cooling pond (4) enters chuck (2) inner cavity
In II (101)；Meanwhile temperature relay (6) control solenoid valve I (42) is opened, the water in chuck (2) inner cavity II (101) is by electricity
Magnet valve I (42) returns in water cooling pond (4).
8. the compound inactivation brood cell's method of pressure thermoacoustic according to claim 7, it is characterised in that：
The method of the supersound process is：
Ultrasonic signal is sent out using ultrasonic generator (72), which is converted to electric signal by ultrasonic transducer (70)
It is transmitted on ultrasonic probe (71), ultrasonic probe (71) occurs in the inner cavity I (100) of disinfection tank (1) according to the signal specific
The vibration of amplitude；
Inner cavity I (100) 1 ± 0.05cm of bottom of the threshold value disinfection tank (1) of the ultrasonic probe (71).
9. the compound inactivation brood cell's method of pressure thermoacoustic according to claim 8, it is characterised in that：
The method of pressurization is：
High-purity nitrogen is injected into the inner cavity I (100) of disinfection tank (1) using nitrogen gas tank (3), and is adjusted by pressure reducing valve (31)
Pressure, until opening relief valve (32) after the completion of sterilizing releases nitrogen.
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| CN109757645A (en) * | 2018-12-14 | 2019-05-17 | 浙江大学 | The method for inducing gemma release DPA to reduce heat resistance |
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