CN109908060A - A kind of raspberry fermentation magma and its preparation method and application - Google Patents
A kind of raspberry fermentation magma and its preparation method and application Download PDFInfo
- Publication number
- CN109908060A CN109908060A CN201910339032.7A CN201910339032A CN109908060A CN 109908060 A CN109908060 A CN 109908060A CN 201910339032 A CN201910339032 A CN 201910339032A CN 109908060 A CN109908060 A CN 109908060A
- Authority
- CN
- China
- Prior art keywords
- raspberry
- preparation
- fermentation magma
- magma
- fermentation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Cosmetics (AREA)
Abstract
The invention belongs to field of biotechnology, and in particular to a kind of raspberry fermentation magma and its preparation method and application.Raspberry fermentation magma is by the way that raspberry, water and zymocyte liquid are mixed to get initial system, carry out fermented and cultured acquisition to initial system.In zymocyte liquid, strain is bacillus amyloliquefaciens LX-12 (Bacillus amyloliquefaciens LX-12), deposit number CCTCC NO:M 2015234.The present invention uses microbe fermentation method, and preparation method is easy, remains the whole components such as raspberry polysaccharide, resveratrol, anthocyanin and its activity；The content of the substances such as raspberry polyphenol, flavones in raspberry magma is also improved simultaneously, and secretes a variety of antibacterial materials, there is natural anti-corrosion effect, growth cycle is short, safe and harmless.Raspberry fermentation magma is applied to cosmetics, it is non-stimulated to skin safe, have effects that remove free radical, anti-oxidant, delaying cell aging and whitening and softening skin.
Description
Technical field
The invention belongs to field of biotechnology, and in particular to a kind of raspberry fermentation magma and its preparation method and application.
Background technique
Raspberry also known as raspberry, rose family rubus plant.Fruit soft and succulency, smell is fragrant and sweet, and nutritive value is abundant.
It is internal certainly with anti-oxidant, removing wherein containing polyphenol substances such as various plants chemical components such as flavones, anthocyanidin, ellagic acid
By base, inhibit the efficacies such as canceration, to cancer, diabetes, obesity, angiocarpy, macular degeneration, neurodegeneration, inflammation and oxygen
Changing the diseases such as damage has inhibiting effect.Raspberry glycosides can be used as novel whitening conditioner simultaneously, inhibits the formation of melanin, catches simultaneously
The nitric oxide in skin is caught, therefore has the effect of whitening and softening skin, anti-aging.
The functional active components of raspberry confirm by domestic and international researcher, the high efficiency extraction of functional active components and effectively
Using increasingly being paid close attention to by people.In plant material, phenolic compound (polyphenol and flavones) often with sugar and glucosides substance shape
At hydroxyl, exist in the form of conjugate, bacillus amyloliquefaciens (Bacillus amyloliquefaciens) can synthesize β-
These conjugates of glucoside enzyme hydrolysis improve the anti-oxidant of plant material to discharge more polyphenol and Flavonoid substances
Activity.Meanwhile B.amyloliquefaciens can secrete a variety of antibacterial materials such as lipopeptid, have natural anti-corrosion effect.This hair
It is bright to be intended to raspberry of fermenting by B.amyloliquefaciens, a kind of raspberry proferment pulp cosmetic and preparation method thereof is provided.
Summary of the invention
In order to overcome the above-mentioned deficiency of the prior art, the present invention provides a kind of raspberry fermentation magma and preparation method thereof and
Using the fermentation magma, using bacillus amyloliquefaciens LX-12 as fermenting microbe, remains in raspberry using raspberry as fermentation raw material
Ingredients and its activity such as anthocyanidin, flavones, raspberry glycosides, have and remove that free radical, anti-oxidant, lifting is compact, whitening and softening skin
Effect.The content that the substances such as raspberry polyphenol, flavones in raspberry magma also can be improved simultaneously, synthesizes a variety of antibacterial materials.
The first purpose of this invention is to propose a kind of preparation method of raspberry fermentation magma, include the following steps: by
Raspberry, water and zymocyte liquid are mixed to get initial system, carry out fermented and cultured to the initial system, obtain raspberry proferment
Slurry；In the zymocyte liquid, strain is bacillus amyloliquefaciens LX-12 (Bacillus amyloliquefaciens LX-
12), deposit number CCTCC NO:M 2015234.
Further, the initial system specific is centrifugated the preparation method comprises the following steps: raspberry and water are mixed with beating into juice
Afterwards, it filters；It collects filtrate to sterilize to obtain raspberry fermentation substrate, then zymocyte liquid is inoculated in raspberry fermentation substrate.
Further, the zymocyte liquid concentration is 3.5 × 108-5×108CFU/mL, the zymocyte liquid is in raspberry
Inoculum concentration in fermentation substrate is 2%-5% (v/v).
Further, in the initial system, the mass ratio of raspberry and water is 1:(1.5-3).
Further, the condition of the fermented and cultured are as follows: fermented and cultured temperature is 30-42 DEG C, fermented incubation time 72-
96h。
Further, the preparation method further include to after the fermented and cultured gained fermentation liquid successively sterilized, from
The heart, take supernatant obtain raspberry fermentation magma the step of.
Further, the condition of the sterilizing are as follows: sterilising temp is 115-121 DEG C, sterilization time 15-25min；It is described
The condition of centrifugation are as follows: centrifugal rotational speed 8000-12000r/min, centrifugation time 15-30min.
Second object of the present invention is the raspberry fermentation for proposing that preparation method described in any of the above embodiments is prepared
Magma.
Third object of the present invention is to propose above-mentioned raspberry fermentation magma directly as with following at least one
Application in the cosmetics of kind function:
(1) anti-oxidant；
(2) free radical is removed；
(3) anti-aging；
(4) whitening.
Fourth object of the present invention is to propose that above-mentioned raspberry fermentation magma has following at least one function in preparation
Application in the cosmetics of energy:
(1) anti-oxidant；
(2) free radical is removed；
(3) anti-aging；
(4) whitening.
Compared with prior art, the beneficial effects of the present invention are:
(1) present invention uses microorganism aging process, by selecting suitable strain and fermentation condition, mentions from raspberry
Get active material.On the one hand, this method does not add any other chemical substance, will not destroy functional active components knot in raspberry
Structure, to remain the whole components such as anthocyanidin in raspberry, tannic acid and its activity to the greatest extent；On the other hand, it does not need to add
Enzyme equal substances, not only save the cost, but also production stage is simplified, preparation is easy, to keep the fermentation technique industrial
Metaplasia produces, and can guarantee the stability of product quality.
(2) it is more that raspberry in raspberry magma can be improved for bacillus amyloliquefaciens LX-12 in the fermenting microbe that the present invention uses
The content of the antioxidant such as phenol, flavones, and a variety of antibacterial materials are secreted, there is natural anti-corrosion effect, growth cycle is short, peace
Completely without evil.
(3) raspberry proferment pulp cosmetic provided by the invention is rich in polyphenol and raspberry glycosides, can remove free radical, delay
Cell ageing inhibits the formation of melanocyte, has effects that lift compact, anti-aging, whitening skin lightening.And product is no added, safety
It is harmless.
Specific embodiment
It shows that example illustrates certain embodiments of the present invention, and should not be construed as limiting model of the invention
It encloses.Present disclosure can be improved from material, method and reaction condition simultaneously, all these improvement should all
It falls within spirit and scope of the invention.
Quantitative experiment in following embodiment, is respectively provided with three repeated experiments, and results are averaged.
Raspberry structure used in the present invention is from Hubei Wuhan fruit supermarket.
Bacillus amyloliquefaciens LX-12 used in the present invention (Bacillus amyloliquefaciens LX-12), preservation
Number CCTCC NO:M 2015234.DPPH structure is from sigma-Aldrich company, the U.S..
LB culture medium: peptone 10g/L, yeast powder 5g/L, sodium chloride 10g/L
Embodiment 1:
1, bacillus bacterium solution is prepared
1) the strain bacillus amyloliquefaciens LX-12 of freezing is inoculated into LB solid medium tablets, 37 DEG C of trainings
12h is supported, its activation is made；
2) by the strain transfer of activation into LB liquid seed culture medium, 37 DEG C, 180rpm cultivates 8-14h, and brood cell is made
Bacillus bacterium solution, the concentration of bacillus LX-12 bacterium solution are 3.5 × 108CFU/mL。
2, preparation raspberry fermentation magma
1) 25g raspberry and 50mL water are mixed with beating into juice, and 4000rpm/min is centrifuged 10min, then with filtered through gauze residue fruit
Slag carries out pasteurization.
2) zymocyte liquid for preparing step 1 is inoculated in raspberry fermentation substrate, 37 according to the inoculum concentration of 2% (v/v)
DEG C, 180rpm cultivates 96h.Fermentation system, as tunning are collected after fermentation.
3) tunning for taking step 2) to obtain, 115-121 DEG C of sterilizing 15-25min.
4) product of step 3) is taken, 10000rpm is centrifuged 20min, collects supernatant, as raspberry fermentation magma.
Embodiment 2:
1, bacillus bacterium solution is prepared
The method of bacillus amyloliquefaciens LX-12 bacterium solution is prepared with embodiment 1, the concentration of bacillus LX-12 bacterium solution is
4.5×108CFU/mL。
2, preparation raspberry fermentation magma
1) 20g raspberry and 30mL water are mixed with beating into juice, and 4000rpm/min is centrifuged 10min, then with filtered through gauze residue fruit
Slag carries out pasteurization.
2) zymocyte liquid for preparing step 1 is inoculated in raspberry fermentation substrate according to the inoculum concentration of 3.5% (v/v),
30 DEG C, 180rpm cultivates 84h.Fermentation system, as tunning are collected after fermentation.
3) tunning for taking step 2) to obtain, 115-121 DEG C of sterilizing 15-25min.
4) product of step 3) is taken, 8000rpm is centrifuged 30min, collects supernatant, as raspberry fermentation magma.
Embodiment 3:
1, bacillus bacterium solution is prepared
The method of bacillus amyloliquefaciens LX-12 bacterium solution is prepared with embodiment 1, the concentration of bacillus LX-12 bacterium solution is 5
×108CFU/mL。
2, preparation raspberry fermentation magma
1) 30g raspberry and 90mL water are mixed with beating into juice, and 4000rpm/min is centrifuged 10min, then with filtered through gauze residue fruit
Slag carries out pasteurization.
2) zymocyte liquid for preparing step 1 is inoculated in raspberry fermentation substrate, 42 according to the inoculum concentration of 5% (v/v)
DEG C, 180rpm cultivates 72h.Fermentation system, as tunning are collected after fermentation.
3) tunning for taking step 2) to obtain, 115-121 DEG C of sterilizing 15-25min.
4) product of step 3) is taken, 12000rpm is centrifuged 15min, collects supernatant, as raspberry fermentation magma.
Embodiment 4: application of the raspberry fermentation magma in cosmetics
One, the property of raspberry fermentation magma
By resulting fermentation magma prepared by embodiment 1-3 through active carbon decoloring, de-taste.Its appearance be thick liquid,
Color is faint yellow to brown color.PH value 6.1-6.8, viscosity 190-230cP, soluble solids content 3.0-3.5%, bacterium colony
Sum is less than 50CFU/mL, no pathogenic bacteria detection.According to hygienic standards for cosmetics GB7916-87, cosmetics total number of bacteria is not high
In 1000CFU/mL, so this fermentation magma meets cosmetics quality requirement.
Two, raspberry fermentation magma constituent analysis
Analysis of antioxidant ingredients is carried out to the raspberry fermentation magma of embodiment 1-3 preparation, wherein fermentation magma is added 10 times
The methanol (m/v) of volume 80% is diluted, and 0.2mL dilute sample is added in the forint phenol reagent (1N) of 0.2mL, reaction
0.4mL Na is added in 3min2CO3Solution (1N) reacts at room temperature 90min, and 2mL deionized water is added, and 725nm measures absorbance, with
Gallic acid is that standard items (10,20,30,40,50 μ g/mL) makes standard curve, and total phenol content is expressed equivalently as mg galla turcica
Acid/mL fermentation magma.The measurement of general flavone content is using rutin as standard items (5,10,15,20,25 μ g/mL), dilution fermentation magma
Sample, detects 258nm absorbance, and general flavone content is expressed equivalently as mg rutin/mL fermentation magma.Acquired results such as table 1.
The fermentation magma constituent analysis of 1 raspberry of table
| General flavone (mg rutin/mL) | Total phenol (mg gallic acid/mL) | |
| Embodiment 1 | 348.14 | 675.87 |
| Embodiment 2 | 375.92 | 719.52 |
| Embodiment 3 | 414.42 | 745.64 |
As shown in Table 1, rich in raspberry magma prepared by the fermenting microbe bacillus amyloliquefaciens LX-12 that the present invention uses
Containing substances such as polyphenol, flavones.
Three, raspberry fermentation magma anti-oxidation efficacy analysis
DPPH is a kind of general organic free radical, the oxidation resistance of assessment antioxidant is often used to, organic
It is more stable in solvent, it is purple, and have special absorption peak at 517nm, when encountering free radical scavenger, the orphan of DPPH is right
Electronics is paired and makes its colour fading, and the peak value of its maximum absorption wavelength is caused to become smaller.Therefore, the reduction of measurement light absorption value can be passed through
Degree evaluates sample to the elimination effect of DPPH free radical, reflects the antioxidant activity of sample.
The step of DPPH radicals scavenging is tested:
1, the raspberry fermentation magma of Example 1-3 is prepared into 10% fermentation magma sample respectively with methanol mixed diluting
Product, for several times, 10000rpm is centrifuged 5min for oscillation.
2, take each 500 μ L of 3 kinds of centrifugates prepared in step 1, respectively with 75 μm of ol/L DPPH- methanol solutions of 2.5mL
Mixing, room temperature are protected from light 90min.Control group is that 500 μ L methanol are mixed with 75 μm of ol/L DPPH- methanol solutions of 2.5mL, room
Temperature is protected from light 90min.
After reaction, absorbance is detected under 517nm.
Clearance rate (%)=(1- sample absorbance/control absorbance) × 100%
Raspberry fermentation magma removes DPPH free radical effect such as table 2.
The raspberry fermentation magma of table 2 10% is to DPPH free radical scavenging activity
| DPPH clearance rate | |
| Embodiment 1 | 82% |
| Embodiment 2 | 90% |
| Embodiment 3 | 93% |
As seen in Table 2, the raspberry fermentation magma prepared in embodiment 1-3 has very strong removing energy to DPPH free radical
Power, clearance rate all reach 80% or more, wherein 10% raspberry fermentation magma prepared by embodiment 3 is clear to DPPH free radical
Except rate has reached 93%.Illustrate that raspberry fermentation magma has the very strong ability for removing free radical, promotes cell metabolism, enhancing is thin
Born of the same parents' vigor improves body mechanism and function, improves body vitality to delay cell senescence and plays the effect of its anti-aging.
Four, raspberry fermentation magma property evaluation safe to the human body
Cosmetics patch test is mainly used for detecting the irritation of cosmetic product.The present invention passes through human closed formula spot
The safety for pasting test evaluation raspberry fermentation magma, assesses its potential skin irritation.
Subjects: 30 volunteers of the range of age at 18-60 years old
Test method: test group: 0.2mL raspberry ferments magma dropwise addition in filter paper, places into spot tester.Blank control
Group: 0.2mL distilled water is added dropwise in another filter paper, is put into spot tester.
Test position is that spot tester is fixed on the outside of volunteer's upper arm with non-stimulated adhesive tape on the outside of upper arm.When test
Between for is for 24 hours for the accuracy of test result, dduring test, volunteer cannot remove spot tester, test position as requested
Water can not be contacted.
It removes spot tester afterwards for 24 hours, stands 30min, disappear to recipient site impression, observe the reaction of skin.If test is
Feminine gender, also need observation for 24 hours, after 48h, the reaction of skin.
Patch test results are as shown in table 1.The meaning that each symbol indicates in table 1 is as follows:
"-" indicates negative reaction；
" ± ", indicates suspicious reaction: only faint erythema；
"+" indicates weakly positive reaction (erythematous response): erythema, oedema, can have papule at infiltration；
" ++ " indicates strong positive reaction (bleb reaction): erythema, infiltration, oedema, papule, bleb；Reaction can exceed tested
Area；
" +++ " indicates pole positive reaction (reaction of amalgamation bleb): obvious erythema, severe infiltration, oedema, amalgamation blister
Rash；Reaction exceeds tested area.
3 patch test results of table
The raspberry fermentation magma that embodiment 1-3 is obtained as can be seen from Table 3 does not generate suspicious reaction, illustrates prepared by the present invention
Raspberry proferment pulp cosmetic has safety, will not bring adverse reaction to human body.
Five, whitening effect is tested
1, method
Raspberry fermentation magma of the invention is actually used, test crowd: totally 60 people, age between 20 to 50 years old, are trying
Original regularity in one's life is kept during testing, test crowd is divided into four groups, and one group is the dark yellow crowd of blee, and two groups are face
There is sunburn caused by primary macule, ultraviolet injury, three groups have the pigmentation after dark sore for face, and four groups have multiple beauty for face
The rebound spot produced after white failure, pigmentation.
2, testing standard
(1) measurement of skin complexion: the skin color tester produced using Courage+Khazaka (CK) company, Germany
Colorimeter CL400 measures the measurement of target area skin complexion L value, passes through the change of skin L value before and after measurement product use
Change to assess influence of the product to the bright degree of skin.
(2) measurement of dermal melanin: using Courage+Khazaka (CK) company, Germany produce dermal melanin with
Ferroheme measuring instrument MX18 measures melanin value in skin, and target area dermal melanin measures 5 times, takes its average value, pass through survey
Dermal melanin numerical value changes to assess the skin whitening efficacy of the product before and after amount product use.
3, test result is shown in Table 4.
4 whitening effect test result of table
4. experimental analysis is summarized
The experimental results showed that when raspberry fermentation magma of the present invention is applied to cosmetics, to crowd's equalization of all kinds of skin quality
Obvious whitening effect.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (10)
1. a kind of preparation method of raspberry fermentation magma, which comprises the steps of: by raspberry, water and zymocyte liquid
It is mixed to get initial system, fermented and cultured is carried out to the initial system, obtains raspberry fermentation magma；In the zymocyte liquid,
Strain be bacillus amyloliquefaciens LX-12 (Bacillus amyloliquefaciens LX-12), deposit number CCTCC NO:
M 2015234。
2. a kind of preparation method of raspberry fermentation magma according to claim 1, which is characterized in that the initial system
Specifically the preparation method comprises the following steps: raspberry and water are mixed with beating into juice, after centrifuge separation, filtering；Filtrate is collected to sterilize to obtain raspberry hair
Ferment substrate, then zymocyte liquid is inoculated in raspberry fermentation substrate.
3. a kind of preparation method of raspberry fermentation magma according to claim 2, which is characterized in that the zymocyte liquid is dense
Degree is 3.5 × 108-5×108CFU/mL, inoculum concentration of the zymocyte liquid in raspberry fermentation substrate are 2%-5% (v/v).
4. according to claim 1 or a kind of preparation methods of 2 described in any item raspberry fermentation magma, which is characterized in that described
In initial system, the mass ratio of raspberry and water is 1:(1.5-3).
5. a kind of preparation method of raspberry fermentation magma according to claim 1, which is characterized in that the fermented and cultured
Condition are as follows: fermented and cultured temperature is 30-42 DEG C, fermented incubation time 72-96h.
6. a kind of preparation method of raspberry fermentation magma according to claim 1, which is characterized in that the preparation method is also
Including successively being sterilized, being centrifuged to gained fermentation liquid after the fermented and cultured, supernatant is taken to obtain the step of raspberry fermentation magma
Suddenly.
7. a kind of preparation method of raspberry fermentation magma according to claim 6, which is characterized in that the condition of the sterilizing
Are as follows: sterilising temp is 115-121 DEG C, sterilization time 15-25min；The condition of the centrifugation are as follows: centrifugal rotational speed 8000-
12000r/min, centrifugation time 15-30min.
8. the raspberry fermentation magma being prepared such as the described in any item preparation methods of claim 1-7.
9. raspberry fermentation magma according to any one of claims 8 is directly as answering in the cosmetics with following at least one functions
With:
(1) anti-oxidant；
(2) free radical is removed；
(3) anti-aging；
(4) whitening.
10. application of the raspberry fermentation magma according to any one of claims 8 in the cosmetics that preparation has following at least one functions:
(1) anti-oxidant；
(2) free radical is removed；
(3) anti-aging
(4) whitening.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910339032.7A CN109908060A (en) | 2019-04-25 | 2019-04-25 | A kind of raspberry fermentation magma and its preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910339032.7A CN109908060A (en) | 2019-04-25 | 2019-04-25 | A kind of raspberry fermentation magma and its preparation method and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109908060A true CN109908060A (en) | 2019-06-21 |
Family
ID=66978553
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910339032.7A Pending CN109908060A (en) | 2019-04-25 | 2019-04-25 | A kind of raspberry fermentation magma and its preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109908060A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114681383A (en) * | 2022-05-20 | 2022-07-01 | 加来(济南)生活科技有限公司 | Sophora flavescens ait fermentation liquor with cutin removing function and fermentation method |
| CN115068394A (en) * | 2022-06-28 | 2022-09-20 | 中北大学 | Whitening toner, emulsion and cream of compound natural plant extract |
| CN116064314A (en) * | 2022-11-01 | 2023-05-05 | 江苏省农业科学院 | Lactobacillus paracasei and application thereof in preparation of fermented blackberry juice with nerve regulating function |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20090132902A (en) * | 2008-06-23 | 2009-12-31 | 조선대학교산학협력단 | Method for preparing fermented liquor using seaweeds and rubus coreanum |
| CN102533576A (en) * | 2010-12-08 | 2012-07-04 | 中国科学院过程工程研究所 | Bacillus amyloliquefaciens LSSE-62 and application thereof |
| CN107348501A (en) * | 2017-09-05 | 2017-11-17 | 桂平市蒙圩镇火炎种养专业合作社 | A kind of preparation method of raspberry ferment |
| CN108740016A (en) * | 2018-04-23 | 2018-11-06 | 蚌埠市和平乳业有限责任公司 | A kind of raspberry fermented yoghourt |
-
2019
- 2019-04-25 CN CN201910339032.7A patent/CN109908060A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20090132902A (en) * | 2008-06-23 | 2009-12-31 | 조선대학교산학협력단 | Method for preparing fermented liquor using seaweeds and rubus coreanum |
| CN102533576A (en) * | 2010-12-08 | 2012-07-04 | 中国科学院过程工程研究所 | Bacillus amyloliquefaciens LSSE-62 and application thereof |
| CN107348501A (en) * | 2017-09-05 | 2017-11-17 | 桂平市蒙圩镇火炎种养专业合作社 | A kind of preparation method of raspberry ferment |
| CN108740016A (en) * | 2018-04-23 | 2018-11-06 | 蚌埠市和平乳业有限责任公司 | A kind of raspberry fermented yoghourt |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114681383A (en) * | 2022-05-20 | 2022-07-01 | 加来(济南)生活科技有限公司 | Sophora flavescens ait fermentation liquor with cutin removing function and fermentation method |
| CN114681383B (en) * | 2022-05-20 | 2023-11-24 | 加来(济南)生活科技有限公司 | Kuh-seng fermentation liquor with exfoliating function and fermentation method |
| CN115068394A (en) * | 2022-06-28 | 2022-09-20 | 中北大学 | Whitening toner, emulsion and cream of compound natural plant extract |
| CN115068394B (en) * | 2022-06-28 | 2023-09-19 | 中北大学 | Whitening toner, emulsion and face cream containing compound natural plant extracts |
| CN116064314A (en) * | 2022-11-01 | 2023-05-05 | 江苏省农业科学院 | Lactobacillus paracasei and application thereof in preparation of fermented blackberry juice with nerve regulating function |
| CN116064314B (en) * | 2022-11-01 | 2024-05-31 | 江苏省农业科学院 | Lactobacillus paracasei and application thereof in preparation of fermented blackberry juice |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109939051A (en) | A kind of mulberries fermentation magma and its preparation method and application | |
| CN105647974B (en) | A kind of saccharomyces cerevisiae plant fermentation object and its application and skin preparations for extenal use | |
| CN107184464B (en) | A kind of Compound Black plant fermentation composition with white-skinned face function and preparation method thereof and the application in facial mask class product | |
| CN113337545B (en) | Schizophyllum commune fermentation product, preparation method thereof, skin care product and schizophyllum commune culture medium | |
| CN109908060A (en) | A kind of raspberry fermentation magma and its preparation method and application | |
| KR101532005B1 (en) | Natural preservative comprising citrus junos seed extract and manufacturing method thereof | |
| CN105434256B (en) | Natural emulsion, preparation method and application thereof | |
| KR101472518B1 (en) | Composition of preservatives for cosmetic and cosmetic composition comprising the same | |
| KR101566320B1 (en) | Cosmetic composition comprising an Impatiens balsamina L, Maca, Rosa multiflora Thunberg, Eryobotrya japonica and Pachyrrhizus erosus Complex-fermented extract | |
| CN108042433B (en) | Whitening antioxidant plant composition, plant composition fermentation liquor, preparation method and application | |
| CN113855603B (en) | Combined fermentation product, skin external preparation containing same, and preparation method and application of combined fermentation product | |
| CN105919889B (en) | A kind of blueness bur proferment pulp cosmetic and the preparation method and application thereof | |
| CN106726949B (en) | Grape seed fermentation raw stock cosmetic and preparation method and application thereof | |
| CN105420132B (en) | A kind of saccharomyces cerevisiae and its preparing the application in skin preparations for extenal use | |
| CN111249218A (en) | Saussurea involucrate fermentation stock solution and preparation method and application thereof | |
| CN109694886A (en) | A kind of green tea fermentation filtrate and its preparation method and application | |
| CN105925617A (en) | Radix pueraiae fermentation protoplasm cosmetic product and preparation method and application thereof | |
| KR101995557B1 (en) | Cosmetic composition including fermented water lily extract and method for manufacturing the same | |
| CN115590800A (en) | Gynura procumbens extract and preparation and application of fermentation extract thereof | |
| KR20160121632A (en) | Manufacturing Method of Fermented Herb Complex Extract for Anti-Oxidant And Low Skin-Irritation | |
| CN110638738B (en) | Method for preparing ecological nutrient solution by treating roses in low-temperature bath and application | |
| CN113208987A (en) | Anti-aging and repairing rice ganoderma lucidum composite fermentation product and preparation method and application thereof | |
| CN109276519A (en) | A kind of paris polyphylla fermenation raw liquid and its preparation method and application | |
| KR20190125005A (en) | Cosmetic composition comprising upland rice fermented extracts | |
| CN116440034A (en) | Preparation method of QILAN tea fermented product and composition for improving skin condition |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190621 |