CN109897774B - A device for quick sterilization of microbial cultivation base - Google Patents

A device for quick sterilization of microbial cultivation base Download PDF

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Publication number
CN109897774B
CN109897774B CN201810899187.1A CN201810899187A CN109897774B CN 109897774 B CN109897774 B CN 109897774B CN 201810899187 A CN201810899187 A CN 201810899187A CN 109897774 B CN109897774 B CN 109897774B
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culture medium
sterilization
reagent chamber
sterilizing agent
sterilizing
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CN109897774A (en
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黄二辉
梁芳芳
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Zhuhai Huikang Biotechnology Co ltd
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Zhuhai Huikang Biotechnology Co ltd
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Abstract

The invention relates to a device for quickly sterilizing a microorganism culture medium, which comprises a culture medium, a crushing device and a sterilizing reagent cavity, wherein a bottle cap is arranged at the upper end of the culture medium, the crushing device and the sterilizing reagent cavity are arranged inside the bottle cap, a sterilizing agent is arranged inside the sterilizing reagent cavity, and the sterilizing agent comprises the components of silver hydrogen peroxide ion disinfectant, sodium hypochlorite, sodium dichloroisocyanurate, chloramine, chlorine dioxide, formaldehyde, glutaraldehyde, phenolic compounds, quaternary ammonium salt compounds, ethanol, iodine, iodophor, hydrogen peroxide and peracetic acid; therefore, the invention has the advantages of small volume, convenient carrying, simple operation, wide application range and no need of professional training.

Description

A device for quick sterilization of microbial cultivation base
Technical Field
The invention belongs to the technical field of culture media and sterilization, and particularly relates to a device for quickly sterilizing a microbial culture medium.
Background
Most of the existing sterilization methods are high-pressure sterilization, r-ray sterilization and the like, and the operation is relatively complex and the time is long; at present, a simple and rapid method for directly sterilizing after microbial culture does not exist, and aiming at the defects, a microbial culture medium and a rapid sterilization method which are small in size, convenient to carry, simple to operate, wide in application range and capable of being completed without professional training are needed to be developed; therefore, the development of a microbial culture medium and a rapid sterilization method which have the advantages of small volume, convenience in carrying, simplicity in operation, wide application range and no need of professional training is of great significance.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide the device for quickly sterilizing the microorganism culture medium, which has the advantages of small volume, convenience in carrying, simplicity in operation, wide application range and no need of professional training.
The purpose of the invention is realized as follows: the utility model provides a device for microbial cultivation base rapid sterilization, it includes culture medium, crushing device and sterilization reagent chamber, the upper end of culture medium is provided with the bottle lid, the inside of bottle lid is provided with crushing device and sterilization reagent chamber, the sterilizing agent has been placed to the inside in sterilization reagent chamber, the composition of sterilizing agent is hydrogen peroxide silver ion antiseptic solution, sodium hypochlorite, dichloro isocyanuric acid sodium, chloramine, chlorine dioxide, formaldehyde, glutaraldehyde, phenol compounds, quaternary ammonium salt compounds, ethanol, iodine and iodophor, hydrogen peroxide and peracetic acid.
The quick sterilization method is characterized in that the crushing device is pressed, the lower end of the sterilization reagent cavity is punctured by the crushing device, a sterilizing agent flows out of the culture medium, and the sterilizing agent can be directly discarded after being uniformly shaken.
The culture medium is a common culture medium or a special culture medium.
The sterile reagent chamber portion is in communication with the culture medium portion via one or more conduits.
The sterile reagent chamber portion is integral with or separate from the vial cap portion.
The sterile reagent chamber portion is one or more.
The invention has the beneficial effects that: the invention has the advantages that the complex process of the post-treatment of the microorganism culture is reduced, after the needed specimen is cultured, the upper part of the bottle cap is directly pressed with force to puncture the lower part of the reagent cavity, and the sterilizing agent flows into the culture medium to kill the microorganism in the culture medium, thereby realizing the harmless treatment; the invention has small volume, convenient carrying, simple operation and wide application range, can be completed without professional training and is suitable for non-professional detection of hospitals, detection mechanisms, family self-test and the like; therefore, the invention has the advantages of small volume, convenient carrying, simple operation, wide application range and no need of professional training.
Drawings
FIG. 1 is a front view of an apparatus for rapid sterilization of a microbial culture medium according to the present invention.
FIG. 2 is a view showing the internal structure of an apparatus for rapid sterilization of a microorganism culture medium according to the present invention.
In the figure: 1. culture medium 2, bottle lid 3, crushing device 4, sterilization reagent chamber.
Detailed Description
The invention is further described below with reference to the accompanying drawings.
Example 1
As shown in fig. 1-2, a device for the rapid sterilization of a microorganism culture medium comprises a culture medium, a crushing device and a sterilization reagent chamber, wherein a bottle cap is arranged at the upper end of the culture medium, the crushing device and the sterilization reagent chamber are arranged inside the bottle cap, a sterilizing agent is arranged inside the sterilization reagent chamber, and the sterilizing agent comprises the components of a silver hydrogen peroxide ion disinfectant, sodium hypochlorite, sodium dichloroisocyanurate, chloramine, chlorine dioxide, formaldehyde, glutaraldehyde, a phenolic compound, a quaternary ammonium salt compound, ethanol, iodine, iodophor, hydrogen peroxide and peracetic acid.
In the invention, the biological culture medium is liquid or semisolid; the sterilizing agent is hydrogen peroxide silver ion disinfectant, chlorine (sodium hypochlorite), sodium dichloroisocyanurate, chloramine, chlorine dioxide, formaldehyde, glutaraldehyde, phenolic compounds, quaternary ammonium salt compounds, ethanol, iodine and iodophor, hydrogen peroxide and peroxyacetic acid; therefore, the invention has the advantages of small volume, convenient carrying, simple operation, wide application range and no need of professional training.
Example 2
As shown in fig. 1-2, a device for rapidly sterilizing a microorganism culture medium comprises a culture medium, a crushing device and a sterilization reagent chamber, wherein a bottle cap is arranged at the upper end of the culture medium, the crushing device and the sterilization reagent chamber are arranged inside the bottle cap, a sterilizing agent is arranged inside the sterilization reagent chamber, the sterilizing agent comprises a silver hydroperoxide ion disinfectant, sodium hypochlorite, sodium dichloroisocyanurate, chloramine, chlorine dioxide, formaldehyde, glutaraldehyde, phenolic compounds, quaternary ammonium salt compounds, ethanol, iodine and iodophor, hydrogen peroxide and peracetic acid, the rapid sterilization method comprises the steps of pressing the crushing device, the crushing device punctures the lower end of the sterilization reagent chamber, the sterilizing agent flows into the culture medium and can be directly discarded after shaking, the culture medium is a common culture medium or a special culture medium, and the sterilization reagent chamber is communicated with the culture medium part through one or more conduits, the sterile reagent chamber portion is integral with or separate from the vial cap portion, and the sterile reagent chamber portion is one or more.
The present invention is further described in the detailed description, rather than by limitation, and it will be apparent to those skilled in the art that numerous changes in structure may be made without departing from the spirit and scope of the invention, all of which are intended to be covered by the appended claims.

Claims (6)

1. A device for the quick sterilization of a microorganism culture medium comprises a culture medium, a crushing device and a sterilization reagent chamber, and is characterized in that: the upper end of culture medium is provided with the bottle lid, the inside of bottle lid is provided with presses brokenly device and sterilization reagent chamber, the sterilizing agent has been placed to the inside in sterilization reagent chamber, the composition of sterilizing agent is hydrogen peroxide silver ion antiseptic solution, sodium hypochlorite, dichloro isocyanuric acid sodium, chloramine, chlorine dioxide, formaldehyde, glutaraldehyde, phenolic compound, quaternary ammonium salt compound, ethanol, iodine and iodophor, hydrogen peroxide and peracetic acid.
2. An apparatus for rapid sterilization of a microbiological culture medium according to claim 1 wherein: the quick sterilization method is characterized in that the crushing device is pressed, the lower end of the sterilization reagent cavity is punctured by the crushing device, a sterilizing agent flows out of the culture medium, and the sterilizing agent can be directly discarded after being uniformly shaken.
3. An apparatus for rapid sterilization of a microbiological culture medium according to claim 1 wherein: the culture medium is a common culture medium or a special culture medium.
4. An apparatus for rapid sterilization of a microbiological culture medium according to claim 1 wherein: the sterile reagent chamber portion is in communication with the culture medium portion via one or more conduits.
5. An apparatus for rapid sterilization of a microbiological culture medium according to claim 1 wherein: the sterile reagent chamber portion is integral with or separate from the vial cap portion.
6. An apparatus for rapid sterilization of a microbiological culture medium according to claim 1 wherein: the sterile reagent chamber portion is one or more.
CN201810899187.1A 2018-08-08 2018-08-08 A device for quick sterilization of microbial cultivation base Active CN109897774B (en)

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CN201810899187.1A CN109897774B (en) 2018-08-08 2018-08-08 A device for quick sterilization of microbial cultivation base

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CN201810899187.1A CN109897774B (en) 2018-08-08 2018-08-08 A device for quick sterilization of microbial cultivation base

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CN109897774A CN109897774A (en) 2019-06-18
CN109897774B true CN109897774B (en) 2022-08-09

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* Cited by examiner, † Cited by third party
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CN114262114A (en) * 2021-11-10 2022-04-01 山东明泰环保科技有限公司 Oriented composite carbon source based on biological decomposition and preparation method thereof

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1174152A (en) * 1980-08-14 1984-09-11 Paula A. Orelski Biological indicator for sterilization processes
CN203794879U (en) * 2012-08-16 2014-08-27 刘占良 Portable microbial culture apparatus for culture before use
CN204474684U (en) * 2015-03-09 2015-07-15 香红星 Spontaneous heating liquid state cultivates the device of active anaerobic microorganism
KR101653911B1 (en) * 2015-03-03 2016-09-05 김수문 Mushroom culture medium sterilizing device using chlorine dioxide
CN107137741A (en) * 2016-03-01 2017-09-08 伊西康公司 The bio-indicator of separate sets

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1174152A (en) * 1980-08-14 1984-09-11 Paula A. Orelski Biological indicator for sterilization processes
CN203794879U (en) * 2012-08-16 2014-08-27 刘占良 Portable microbial culture apparatus for culture before use
KR101653911B1 (en) * 2015-03-03 2016-09-05 김수문 Mushroom culture medium sterilizing device using chlorine dioxide
CN204474684U (en) * 2015-03-09 2015-07-15 香红星 Spontaneous heating liquid state cultivates the device of active anaerobic microorganism
CN107137741A (en) * 2016-03-01 2017-09-08 伊西康公司 The bio-indicator of separate sets

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