CN109897774A - A kind of microbiological culture media and quick sterilization method - Google Patents
A kind of microbiological culture media and quick sterilization method Download PDFInfo
- Publication number
- CN109897774A CN109897774A CN201810899187.1A CN201810899187A CN109897774A CN 109897774 A CN109897774 A CN 109897774A CN 201810899187 A CN201810899187 A CN 201810899187A CN 109897774 A CN109897774 A CN 109897774A
- Authority
- CN
- China
- Prior art keywords
- culture medium
- culture media
- sterilizing
- sterilization method
- quick sterilization
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The invention is related to a kind of microbiological culture media and quick sterilization method, it includes culture medium, crush device and sterilizing reagent chamber, the upper end of the culture medium is provided with bottle cap, being internally provided with for the bottle cap crushes device and sterilizing reagent chamber, the inside of the sterilizing reagent chamber is placed with bactericidal agent, the constituent of the bactericidal agent is hydrogen peroxide silver ion thimerosal, chlorine (sodium hypochlorite), sodium dichloro cyanurate, chloramines, chlorine dioxide, formaldehyde, glutaraldehyde, phenolic compound, quaternary ammonium compound, ethyl alcohol, iodine and Iodophor, hydrogen peroxide and Peracetic acid；Therefore, the present invention has the advantages that small in size, easy to carry, easy to operate, the scope of application is wide, can be completed without professional training.
Description
Technical field
The invention belongs to culture medium and the technical fields of sterilizing, and in particular to a kind of microbiological culture media and quick sterilization side
Method.
Background technique
Present sterilizing methods are mostly high pressure sterilization, r ray sterilizing etc., and operation is relative complex, and the time is long；Currently without one
A kind of simple and quick method that can directly sterilize after microculture, for these shortcomings, it is necessary to develop volume
It is small, easy to carry, easy to operate, the scope of application is wide, the microbiological culture media and quick sterilization that can be completed without professional training
Method；Therefore, develop it is a kind of it is small in size, easy to carry, easy to operate, the scope of application is wide, can be completed without professional training
Microbiological culture media and quick sterilization method have a very important significance.
Summary of the invention
The purpose of the invention is to overcome the deficiencies in the prior art, and provide a kind of small in size, easy to carry, operation letter
It is single, the scope of application is wide, the microbiological culture media that can be completed without professional training and quick sterilization method.
The object of the present invention is achieved like this: a kind of microbiological culture media and quick sterilization method, it include culture medium,
Device and sterilizing reagent chamber are crushed, the upper end of the culture medium is provided with bottle cap, and being internally provided with for the bottle cap crushes device
With sterilizing reagent chamber, the inside of the sterilizing reagent chamber is placed with bactericidal agent, and the constituent of the bactericidal agent is hydrogen peroxide
Silver ion thimerosal, chlorine (sodium hypochlorite), sodium dichloro cyanurate, chloramines, chlorine dioxide, formaldehyde, glutaraldehyde, phenols chemical combination
Object, quaternary ammonium compound, ethyl alcohol, iodine and Iodophor, hydrogen peroxide and Peracetic acid.
The quick sterilization method is to crush device, crushes device and punctures the lower end for the reagent chamber that sterilizes, bactericidal agent
It flows out in culture medium, can directly be abandoned after shaking up.
The culture medium is ordinary culture medium or special culture media.
The sterilizing reagent cavity segment is connected to by one or more conduit with culture medium part.
The sterilizing reagent cavity segment integrally or is separated with covering part.
The sterilizing reagent cavity segment is one or more.
Beneficial effects of the present invention: benefit of the invention is to reduce the complex process of microculture post-processing, by institute
After needing sample culture, the pressing bottle side of covering of directly exerting oneself punctures reagent chamber lower part, and bactericidal agent flows into culture medium, kills culture
Microorganism in base, accomplishes harmless treatment；The present invention is small in size, and easy to carry, easy to operate, the scope of application is wide, without special
Industry training can be completed, and is suitble to hospital, testing agency and family to detect from laymans such as surveys and uses；Therefore, the present invention has
It is small in size, easy to carry, easy to operate, the scope of application is wide, the advantages of can be completed without professional training.
Detailed description of the invention
Fig. 1 is the main view of a kind of microbiological culture media of the present invention and quick sterilization method.
Fig. 2 is the internal structure chart of a kind of microbiological culture media of the present invention and quick sterilization method.
In figure: 1, culture medium 2, bottle cap 3, crush device 4, sterilizing reagent chamber.
Specific embodiment
Following further describes the present invention with reference to the drawings.
Embodiment 1
As shown in Figs. 1-2, a kind of microbiological culture media and quick sterilization method, it includes culture medium, crushes device and go out
Bacterium reagent chamber, the upper end of the culture medium are provided with bottle cap, and being internally provided with for the bottle cap crushes device and sterilizing reagent chamber,
The inside of the sterilizing reagent chamber is placed with bactericidal agent, the constituent of the bactericidal agent be hydrogen peroxide silver ion thimerosal,
Chlorine (sodium hypochlorite), sodium dichloro cyanurate, chloramines, chlorine dioxide, formaldehyde, glutaraldehyde, phenolic compound, quaternary ammonium salt chemical combination
Object, ethyl alcohol, iodine and Iodophor, hydrogen peroxide and Peracetic acid.
In the present invention, biological medium is liquid or semisolid；Bactericidal agent is hydrogen peroxide silver ion thimerosal, chlorine is (secondary
Sodium chlorate), sodium dichloro cyanurate, chloramines, chlorine dioxide, formaldehyde, glutaraldehyde, phenolic compound, quaternary ammonium compound, second
Alcohol, iodine and Iodophor, hydrogen peroxide and Peracetic acid；Therefore, the present invention has small in size, easy to carry, easy to operate, applicable model
The advantages of enclosing width, can be completed without professional training.
Embodiment 2
As shown in Figs. 1-2, a kind of microbiological culture media and quick sterilization method, it includes culture medium, crushes device and go out
Bacterium reagent chamber, the upper end of the culture medium are provided with bottle cap, and being internally provided with for the bottle cap crushes device and sterilizing reagent chamber,
The inside of the sterilizing reagent chamber is placed with bactericidal agent, the constituent of the bactericidal agent be hydrogen peroxide silver ion thimerosal,
Chlorine (sodium hypochlorite), sodium dichloro cyanurate, chloramines, chlorine dioxide, formaldehyde, glutaraldehyde, phenolic compound, quaternary ammonium salt chemical combination
Object, ethyl alcohol, iodine and Iodophor, hydrogen peroxide and Peracetic acid, the quick sterilization method are to crush device, and crushing device will
The lower end of sterilizing reagent chamber punctures, and bactericidal agent flows out in culture medium, can directly abandon after shaking up, and the culture medium is general
Logical culture medium or special culture media, the sterilizing reagent cavity segment are connected by one or more conduit and culture medium part
Logical, the sterilizing reagent cavity segment integrally or is separated with covering part, and the sterilizing reagent cavity segment is one or more.
Specific embodiment is to further explanation of the invention rather than limits, and is existed for those of ordinary skills
Further transformation is done to structure in the case where not departing from substantive content of the present invention, and all these transformation all should belong to institute of the present invention
Attached scope of protection of the claims.
Claims (6)
1. a kind of microbiological culture media and quick sterilization method, it includes culture medium, crushes device and sterilizing reagent chamber, feature
Be: the upper end of the culture medium is provided with bottle cap, and being internally provided with for the bottle cap crushes device and sterilizing reagent chamber, described
The inside of sterilizing reagent chamber is placed with bactericidal agent, and the constituent of the bactericidal agent is hydrogen peroxide silver ion thimerosal, chlorine is (secondary
Sodium chlorate), sodium dichloro cyanurate, chloramines, chlorine dioxide, formaldehyde, glutaraldehyde, phenolic compound, quaternary ammonium compound, second
Alcohol, iodine and Iodophor, hydrogen peroxide and Peracetic acid.
2. a kind of microbiological culture media according to claim 1 and quick sterilization method, it is characterised in that: described quickly to go out
Bacterium method is to crush device, crushes device and punctures the lower end for the reagent chamber that sterilizes, bactericidal agent flows out in culture medium, shakes up
It can directly abandon later.
3. a kind of microbiological culture media according to claim 1 and quick sterilization method, it is characterised in that: the culture medium
For ordinary culture medium or special culture media.
4. a kind of microbiological culture media according to claim 1 and quick sterilization method, it is characterised in that: the sterilizing examination
Agent cavity segment is connected to by one or more conduit with culture medium part.
5. a kind of microbiological culture media according to claim 1 and quick sterilization method, it is characterised in that: the sterilizing examination
Agent cavity segment integrally or is separated with covering part.
6. a kind of microbiological culture media according to claim 1 and quick sterilization method, it is characterised in that: the sterilizing examination
Agent cavity segment is one or more.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810899187.1A CN109897774B (en) | 2018-08-08 | 2018-08-08 | A device for quick sterilization of microbial cultivation base |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810899187.1A CN109897774B (en) | 2018-08-08 | 2018-08-08 | A device for quick sterilization of microbial cultivation base |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109897774A true CN109897774A (en) | 2019-06-18 |
| CN109897774B CN109897774B (en) | 2022-08-09 |
Family
ID=66943151
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810899187.1A Active CN109897774B (en) | 2018-08-08 | 2018-08-08 | A device for quick sterilization of microbial cultivation base |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109897774B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114262114A (en) * | 2021-11-10 | 2022-04-01 | 山东明泰环保科技有限公司 | Oriented composite carbon source based on biological decomposition and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1174152A (en) * | 1980-08-14 | 1984-09-11 | Paula A. Orelski | Biological indicator for sterilization processes |
| CN203794879U (en) * | 2012-08-16 | 2014-08-27 | 刘占良 | Portable microbial culture apparatus for culture before use |
| CN204474684U (en) * | 2015-03-09 | 2015-07-15 | 香红星 | Spontaneous heating liquid state cultivates the device of active anaerobic microorganism |
| KR101653911B1 (en) * | 2015-03-03 | 2016-09-05 | 김수문 | Mushroom culture medium sterilizing device using chlorine dioxide |
| CN107137741A (en) * | 2016-03-01 | 2017-09-08 | 伊西康公司 | The bio-indicator of separate sets |
-
2018
- 2018-08-08 CN CN201810899187.1A patent/CN109897774B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1174152A (en) * | 1980-08-14 | 1984-09-11 | Paula A. Orelski | Biological indicator for sterilization processes |
| CN203794879U (en) * | 2012-08-16 | 2014-08-27 | 刘占良 | Portable microbial culture apparatus for culture before use |
| KR101653911B1 (en) * | 2015-03-03 | 2016-09-05 | 김수문 | Mushroom culture medium sterilizing device using chlorine dioxide |
| CN204474684U (en) * | 2015-03-09 | 2015-07-15 | 香红星 | Spontaneous heating liquid state cultivates the device of active anaerobic microorganism |
| CN107137741A (en) * | 2016-03-01 | 2017-09-08 | 伊西康公司 | The bio-indicator of separate sets |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114262114A (en) * | 2021-11-10 | 2022-04-01 | 山东明泰环保科技有限公司 | Oriented composite carbon source based on biological decomposition and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109897774B (en) | 2022-08-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Akey et al. | Liquid-phase study of ozone inactivation of Venezuelan equine encephalomyelitis virus | |
| Lin et al. | The antibacterial efficacy and mechanism of plasma-activated water against salmonella enteritidis (ATCC 13076) on shell eggs | |
| CN108102947A (en) | One plant of aerobic-type efficient calcium mineralising bacillus and the application in concrete reparation | |
| CN104322554A (en) | Novel electric potential water disinfectant and preparation method thereof | |
| CN109897774A (en) | A kind of microbiological culture media and quick sterilization method | |
| CN203354943U (en) | Harmless treatment box for waste bacterial liquor of petroleum microorganisms | |
| Wan et al. | Enhanced solar inactivation of fungal spores by addition of low-dose chlorine: Efficiency and mechanism | |
| Wan et al. | Occurrence and control of fungi in water: new challenges in biological risk and safety assurance | |
| Somani et al. | Performance evaluation of natural herbs for antibacterial activity in water purification | |
| US2374805A (en) | Sterilization of water for drinking purposes | |
| Martirosyan et al. | Carbon dioxide as a microbial toxicity enhancer of some antibacterial agents: a new potential water purification tool | |
| CN101592612A (en) | Test paper for rapidly checking residual content of sterilizing agent | |
| CN103382440A (en) | Safe inoculation device of liquid fermentation tank | |
| Edmondson et al. | Simple methods of bacteriologic sampling of nebulization equipment | |
| DE2614542C3 (en) | Removal of reaction material from sterile processes and device for its implementation | |
| CN206672154U (en) | A kind of paper money counter with ozone sterilizing function | |
| CN202920674U (en) | Microwave sterilizer for nutrient solution | |
| CN209662268U (en) | A kind of microbiological culture media Quick sterilizing device | |
| CN203715633U (en) | Anti-pollution portable bacterium inoculating loop | |
| CN202834174U (en) | Manual sterile sampling valve structure | |
| Shi et al. | Inactivation effect of low-temperature plasma on pseudomonas aeruginosa for nosocomial anti-infection | |
| CN111226946A (en) | Bactericidal disinfectant and preparation method thereof | |
| CN103285958A (en) | Portable anaerobic experiment device for degrading environmental organic pollutants | |
| CN109701053B (en) | Disinfection method | |
| CN202576430U (en) | Electrode replacing device of bioreactor |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |