CN109874855A - A kind of method of feruloylated oligosaccharides keeping fresh aquatic products - Google Patents
A kind of method of feruloylated oligosaccharides keeping fresh aquatic products Download PDFInfo
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- CN109874855A CN109874855A CN201910187413.8A CN201910187413A CN109874855A CN 109874855 A CN109874855 A CN 109874855A CN 201910187413 A CN201910187413 A CN 201910187413A CN 109874855 A CN109874855 A CN 109874855A
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- feruloylated oligosaccharides
- aquatic products
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- oligosaccharides
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Abstract
The invention belongs to feruloylated oligosaccharides application technology as the second resource fields, and in particular to a kind of method of feruloylated oligosaccharides keeping fresh aquatic products.The specific steps of the method are as follows: the pretreatment of raw material: fresh grass carp decaptitating, tail, squama, fin and internal organ rinse residual clot and spot and drain naturally, and stripping and slicing is spare；Preservation Treatment: preparing feruloylated oligosaccharides solution, and by treated, grass carp is soaked in feruloylated oligosaccharides solution, refrigeration or cold storage after immersion treatment.This method can effectively extend the shelf life of grass carp, improve the quality of the flesh of fish.
Description
Technical field
The invention belongs to feruloylated oligosaccharides application technology as the second resource fields, and in particular to a kind of feruloylated oligosaccharides are fresh-keeping
The method of aquatic products.
Background technique
Grass carp (Grass carp) is commonly called as sea otter, black black carp etc. in south, and full of nutrition, consumption figure and cultured output are equal
It occupies first of fresh-water fishes.Grass carp lacks the semi-finished product or converted products of mature sizing mainly based on fresh and alive sale in the market at present.
With increasing sharply for cultured output and the market demand, single fresh and alive marketing method has been difficult to meet the market demand, therefore,
Exploitation has important realistic meaning without voluntarily cuing open the prefinished products killed.But since in storage and transportation process, the flesh of fish is in self-dissolving
Easily cause corruption under the action of enzyme and bacterium, therefore preservation technology just seems and is even more important.Method for preserving aquatic products master at present
If preservation by low temperature, superhigh pressure fresh-keeping and fresh chemically etc..Under the conditions of preservation by low temperature, the intracorporal microorganism of fish and enzymatic activity are not
It is totally constrained, corruption is still continuing；Superhigh pressure fresh-keeping need to provide special installation, fresh-keeping higher cost；Fresh chemically is to people
There are potential dangers for health, and therefore, the exploitation of bio-preservative has been increasingly becoming the hot spot of preservation of fishery research with application.
Currently, multi-purpose greatly tea polyphenols, phytic acid etc. are used as crude antistaling agent in industry.And water-retaining agent mostly uses sucrose, compound
Phosphate etc., though having preferable cryoprotective effects to the flesh of fish, the characteristics of its high fever high sweet tea, does not meet the consumption requirement of low sweet tea low-heat,
Taking in excessive phosphate will lead to osteoporosis diseases.Feruloylated oligosaccharides (Feruloyl oligosaccharides,
FOs) it is a kind of functional oligose that the carboxyl of ferulic acid and the hydroxyl of oligosaccharide are formed by connecting by ester bond.FOs is due to it
Special ester bond in structure, so that certain physiological activity are enhanced.Research shows that FOs is in addition to can lowering blood pressure and blood fat, anti-swollen
Tumor, inhibits outside the intracorporal glycosylation of people anti-aging, also has stronger antioxidant activity and biocidal property.In addition, China is also
It is a Beer Brewage big country, can all generates every year a large amount of brewex's grains (Brewer ' s spent grain, BSG), and beer
Main component is protein and dietary fiber in grain, and is to prepare one of raw material of FOs containing 0.4% or so ferulic acid.But
Nowadays brewex's grains directly are used to raise animal or drain into river by many enterprises, so that ecological environment becomes more severe, simultaneously
Also result in a large amount of wastes of brewex's grains.Therefore, how brewex's grains rationally effectively to be utilized, it has also become the industry institute such as brewery face
The great difficult problem faced.
Structure etc. is more concentrated on for the research of feruloylated oligosaccharides at present, the fresh-keeping water conservation of grass carp is applied also not
It appears in the newspapers.Therefore, by the FOs of brewex's grains fermentation preparation, its fresh-keeping water retention to grass carp fish block is probed into, this is using useless
The brewex's grains exploitation natural fresh-keeping water-retaining agent of abandoning provides feasibility.
Summary of the invention
In order to overcome the shortcomings in the prior art, the object of the present invention is to provide a kind of feruloylated oligosaccharides for present invention offer
The method of keeping fresh aquatic products；This method can effectively extend the shelf life of grass carp, improve the quality of the flesh of fish.
In order to achieve the above object, the present invention is achieved by the following technical programs:
A kind of method of feruloylated oligosaccharides keeping fresh aquatic products, comprising the following steps:
(1) pretreatment of raw material: fresh grass carp decaptitating, tail, squama, fin and internal organ rinse residual clot and spot and drain naturally,
Stripping and slicing is spare；
(2) Preservation Treatment: preparing feruloylated oligosaccharides solution, will that grass carp is soaked in asafoetide acyl is low by step (1) treated
In glycan solution, refrigeration or cold storage after immersion treatment.
As a preferred solution of the present invention, the mass-volume concentration of the feruloylated oligosaccharides solution is 3-9 g/L.
As a preferred solution of the present invention, the refrigerated storage temperature is 4 DEG C, and cold preservation time is 12 d；The storage temperature be-
20 DEG C, the refrigeration time is 30-60 d.
As a preferred solution of the present invention, the feruloylated oligosaccharides are made after isolating and purifying by solid state fermentation brewex's grains
It is standby to obtain.
As a preferred solution of the present invention, the mass-volume concentration of the feruloylated oligosaccharides solution is 6 g/L.
As a preferred solution of the present invention, the feruloylated oligosaccharides specific the preparation method is as follows:
(1) pretreatment of brewex's grains: brewex's grains are dried in 60 DEG C, crushed 60 meshes:
(2) preparation of solid medium: weighing through step (1) treated 20 g of brewex's grains, be separately added into 1.0 g of xylan,
0.6 g of urea, 0.1 g of potassium dihydrogen phosphate, distilled water 40 mL, pH are naturally, obtain solid medium；
(3) the resulting culture of step (2) is based on 121 DEG C of 20 min of high-temperature sterilization；The Aureobasidium pullulans kind of inoculation 12% after cooling
Sub- liquid cultivates 4 d in 30 DEG C of constant incubators, obtains tunning:
(4) tunning extracts 60 under 28 DEG C, 250 r/min with the citrate-phosphate buffer of 10 times of 50 mM
Min, centrifugation, supernatant cross miillpore filter, filtrate concentration；Elution, collects 50% methanol elution fraction, and concentration is freeze-dried to obtain
To feruloylated oligosaccharides.
As a preferred solution of the present invention, the pH of citrate-phosphate buffer is 5.0 in the step (4).
Wherein, zytase, amylase, protease, cellulase etc. are produced in Aureobasidium pullulans growth course.Wood is poly-
Carbohydrase is the key enzyme for producing FOs, amylase and the starch in protease hydrolytic raw material, the growth that protein is Aureobasidium pullulans
Nutrition is provided, meanwhile, the microbe application for producing zytase directly can be made into enzymatic production process and enzyme in FOs preparation
Solving xylan and obtaining the process two of FOs is one, can save purification procedures and insoluble diedairy fiber in enzyme preparation production
Preparation step, reduce cost.In addition, solid state fermentation mode can reduce a large amount of discharges of waste water, and its low water environment has
Conducive to purebred culture, living contaminants are avoided.
Compared with prior art, the invention has the following advantages:
(1) feruloylated oligosaccharides in the present invention have the bioactivity and antioxidant activity of multiplicity；Storage period pass through Ah
The film that Wei's acyl oligosaccharide is formed on flesh of fish surface realizes feruloylated oligosaccharides to the fresh-keeping water retention of the grass carp flesh of fish；
(2) feruloylated oligosaccharides in the present invention are when concentration is 0.60%, i.e. the mass-volume concentration of feruloylated oligosaccharides solution
It is best to the fresh-keeping Water-saving effect of the grass carp flesh of fish when for 6 g/L；Defrosting loss late is lower than 4.11 percentage points of distilled water group, steams
Loss late is boiled lower than 12.19 percentage points of distilled water group.
(3) the features such as present invention has process simple, significant effect can mention for the higher value application of feruloylated oligosaccharides
For theoretical foundation.
Detailed description of the invention
Fig. 1 is the variation of fish block total plate count within the refrigeration phase during the fresh-keeping grass carp of feruloylated oligosaccharides；
Fig. 2 is the variation of fish block pH value within the refrigeration phase during the fresh-keeping grass carp of feruloylated oligosaccharides.
Specific embodiment
Below by specific embodiment, invention is further described in detail.But those skilled in the art will manage
Solution, the following example is merely to illustrate the present invention, and should not be taken as limiting the scope of the invention.Specific skill is not specified in embodiment
Art or condition person, described technology or conditions carry out to specifications according to the literature in the art.Agents useful for same or instrument
Production firm person is not specified, being can be with conventional products that are commercially available.
Embodiment 1
A kind of method of feruloylated oligosaccharides keeping fresh aquatic products, comprising the following steps:
(1) sample treatment
Fresh grass carp decaptitating, tail, squama, fin, internal organ, are placed in refrigerating box and transport laboratory back rapidly, and aseptic water washing remains clot
And spot, it is drained naturally in aseptic operating platform.It is cut into the fish block of 30 g or so, it is spare；
(2) feruloylated oligosaccharides solution and sample impregnate
The feruloylated oligosaccharides of 0.30 g, 0.60 g and 0.90 g are taken to be settled to 100 mL respectively, obtaining mass volume ratio is respectively
0.30%, 0.60%, 0.90% feruloylated oligosaccharides solution；4 equal portions of fish block sample for weighing phase homogenous quantities, are distilled respectively
Water immersion treatment, 0.30% FOs, 0.60% FOs and 0.90% FOs immersion treatment, soaking temperature maintain 4 DEG C, impregnate 30
Sample is taken out after min, in draining on sterile gauze, every equal portions are divided into 2 equal portions again and are packed into valve bag, be subsequently placed into 4 DEG C and-
It is stored at a temperature of 20 DEG C.
Wherein, the feruloylated oligosaccharides the preparation method comprises the following steps: brewex's grains in 60 DEG C dry, crushed 60 meshes.Solid-state
Culture medium: weighing 20 g of brewex's grains, is separately added into 1.0 g of xylan, 0.6 g of urea, 0.1 g of potassium dihydrogen phosphate, distilled water 40
ML, pH are natural.Culture is based on 121 DEG C of 20 min of high-temperature sterilization.After culture medium is cooling, it is inoculated with 12% Aureobasidium pullulans seed
Liquid cultivates 4 d in 30 DEG C of constant incubators.The citrate-phosphate buffer of 10 times of 50 mM of tunning
(pH5.0) 60 min are extracted under 28 DEG C, 250 r/min, are centrifuged, and supernatant crosses 0.45 μm of miillpore filter, filtrate concentration.It is dense
Amberlite XD-2 column (40 × 2.5 cm) on contracting liquid, successively with 50% first of the distilled water of 2 times of column volumes, 3 times of column volumes
The elution of the anhydrous methanol of alcohol solution and times column volume, collects 50% methanol elution fraction, and concentration is freeze-dried to obtain FOs.
Sample of the embodiment 1 after fresh-keeping is measured into indices, all indexs carry out 3 parallel tests.
The measuring method of fresh-keeping index are as follows: the fish block stored at a temperature of 4 DEG C is rubbed, after homogenate, measures total plate count, pH
Value, TVB-N content and TBARS value；
It is retained the measuring method of index are as follows: the fish block stored at a temperature of -20 DEG C is taken out, after 4 h that thaw in 4 DEG C of environment,
Measure defrosting loss late and cooking loss rate, wherein 3 min of water proof boiling is needed when boiling.
The measurement of total plate count and pH:
(1) flesh of fish for taking 5.0 g to rub, is placed in 45 ml sterile salines, will with 10 times of amount dilution methods sufficiently after oscillation
Sample liquid continues to dilute, and selects 3 suitable dilutions, respectively takes 1 ml dilution that nutrient agar panel is added, is coated, 3
Duplicate Samples after cultivating 48 h in 36 DEG C of constant incubators, measure clump count with colony counting method.It is with the physiological saline of sterilizing
Blank does control experiment.
(2) 5.0 g of grass carp sample after rubbing is weighed in beaker, and 45 mL of addition boil cooling distilled water and uniformly stir
30 min are impregnated after mixing, are filtered, and filtrate is measured using pH meter, is repeated 3 times and is averaged.
(3) it statisticallys analyze
Referring to Fig.1, in the present invention, four groups of total plate count with the extension of storage time it is in rising trend, but FOs handle
The total plate count of group is significantly less than control group.When 6 d, the total plate count of control group is up to 6.43 lg CFU/g, has exceeded food
Just exceed 6 lg CFU/g in 9 d with the total plate count of standard, and FOs processing group, illustrates that FOs has apparent antibacterial work
With.Wherein the total plate count of 0.60% FOs processing group is substantially less than 0.30% and 0.90% FOs group, illustrates that 0.60% FOs is handled
The shelf life of group extends 3 d compared to control group.
Referring to Fig. 2, in the present invention, the pH of fish block is presented in storage fall before after the trend that rises.At FOs
After reason, the variation of the pH value of fish block is slower, substantially less than control group.Wherein, particularly evident with 0.60% FOs processing group, and
Its pH value ascendant trend is gentle.In conjunction with Fig. 1 analysis it is found that the total plate count of 0.60% FOs processing group is less than other groups, cause thin
Bacterium decomposes the flesh of fish and generates the degree of alkaline nitrogen substance much smaller than other processing groups.
Conclusion: the present embodiment is measured the flesh of fish using pH method using the variation of colony counting method measurement total plate count
PH value.The result shows that the flesh of fish through feruloylated oligosaccharides immersion treatment is significantly mentioned compared with the flesh of fish fresh-keeping effect that distilled water immersion is handled
It is high.
The above is only preferred embodiments of the present invention, is not intended to limit the scope of the present invention,
Therefore any trickle amendment, equivalent variations and modification made to the above embodiment according to the technical essence of the invention, belong to
In the range of technical solution of the present invention.
Claims (7)
1. a kind of method of feruloylated oligosaccharides keeping fresh aquatic products, which comprises the following steps:
(1) pretreatment of raw material: fresh grass carp decaptitating, tail, squama, fin and internal organ rinse residual clot and spot and drain naturally,
Stripping and slicing is spare；
(2) Preservation Treatment: preparing feruloylated oligosaccharides solution, will that grass carp is soaked in asafoetide acyl is low by step (1) treated
In glycan solution, refrigeration or cold storage after immersion treatment.
2. a kind of method of feruloylated oligosaccharides keeping fresh aquatic products according to claim 1, which is characterized in that Ah
The mass-volume concentration of Wei's acyl oligosaccharide solution is 3-9 g/L.
3. a kind of method of feruloylated oligosaccharides keeping fresh aquatic products according to claim 1, which is characterized in that described cold
Hiding temperature is 4 DEG C, cold preservation time 12d；The storage temperature is -20 DEG C, and the refrigeration time is 30-60 d.
4. a kind of method of feruloylated oligosaccharides keeping fresh aquatic products according to claim 1, which is characterized in that the asafoetide
Acyl oligosaccharide is prepared after isolating and purifying by solid state fermentation brewex's grains.
5. a kind of method of feruloylated oligosaccharides keeping fresh aquatic products according to claim 2, which is characterized in that Ah
The mass-volume concentration of Wei's acyl oligosaccharide solution is 6 g/L.
6. a kind of method of feruloylated oligosaccharides keeping fresh aquatic products according to claim 4, which is characterized in that the asafoetide
Acyl oligosaccharide specific the preparation method is as follows:
(1) pretreatment of brewex's grains: brewex's grains are dried in 60 DEG C, crushed 60 meshes:
(2) preparation of solid medium: weighing through step (1) treated 20 g of brewex's grains, be separately added into 1.0 g of xylan,
0.6 g of urea, 0.1 g of potassium dihydrogen phosphate, distilled water 40 mL, pH are naturally, obtain solid medium；
(3) the resulting culture of step (2) is based on 121 DEG C of 20 min of high-temperature sterilization；The Aureobasidium pullulans kind of inoculation 12% after cooling
Sub- liquid cultivates 4 d in 30 DEG C of constant incubators, obtains tunning:
(4) tunning extracts 60 under 28 DEG C, 250 r/min with the citrate-phosphate buffer of 10 times of 50 mM
Min, centrifugation, supernatant cross miillpore filter, filtrate concentration；Elution, collects 50% methanol elution fraction, and concentration is freeze-dried to obtain
To feruloylated oligosaccharides.
7. a kind of method of feruloylated oligosaccharides keeping fresh aquatic products according to claim 6, which is characterized in that the step
(4) pH of citrate-phosphate buffer is 5.0 in.
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Cited By (3)
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| CN112493428A (en) * | 2020-11-30 | 2021-03-16 | 浙江海洋大学 | Pretreatment method of canned portunus trituberculatus crab meat |
| CN113925160A (en) * | 2021-09-24 | 2022-01-14 | 盐城工学院 | Microcapsule with oxidation resistance and preparation method thereof |
| CN115428830A (en) * | 2022-08-18 | 2022-12-06 | 盐城工学院 | Method for promoting self-healing of mechanically damaged fruits based on functional sugar self-assembly membrane and application of method |
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Application publication date: 20190614 |