CN109855529A - The acquisition methods of digital hologram three-dimensional microscopic system and photo chromic microimage texture - Google Patents
The acquisition methods of digital hologram three-dimensional microscopic system and photo chromic microimage texture Download PDFInfo
- Publication number
- CN109855529A CN109855529A CN201711237135.XA CN201711237135A CN109855529A CN 109855529 A CN109855529 A CN 109855529A CN 201711237135 A CN201711237135 A CN 201711237135A CN 109855529 A CN109855529 A CN 109855529A
- Authority
- CN
- China
- Prior art keywords
- light
- dimensional
- digital hologram
- light source
- source
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The present invention proposes the acquisition methods of a kind of digital hologram three-dimensional microscopic system and photo chromic microimage texture, the digital hologram three-dimensional microscopic system includes that can emit the monochromatic source of linearly polarized light, the monochromatic source can be emitted beam and set up the spectrophotometric unit for being formed as reference light Yu object light two-beam separately, and the light combination unit that reference light can be merged with object light, the other side of the light combination unit is provided with the digital camera sensor that can acquire interference fringe or color texture image, it is formed between the spectrophotometric unit and the light combination unit referring to optical transport optical path and object optical transport optical path, the starting point of the object optical transport optical path is provided with the color picture light source that can start when acquiring color texture image；The acquisition methods of the photo chromic microimage texture are based on above-mentioned digital hologram three-dimensional microscopic system.The ultraprecise digital hologram three-dimensional measurement for having photo chromic microimage texture of high speed is realized in the invention.
Description
Technical field
The invention belongs to Digital Holography field, more particularly to a kind of number that can obtain photo chromic microimage are complete
Cease three-dimensional microscopic system.
Background technique
Digital hologram is using the method for the digitlization light field reconstruction of optical interference and diffraction principle, it is by reflected light
Opaque article surface is regarded as through the point in each three-dimensional space in the transparent of light, translucent object internal structure
These point light source light-emittings time is calculated using computer by the mathematical formulae of optical interference and diffraction for a point light source
Wave phase realizes that carrying out nanoscale to opaque article surface or transparent, translucency shape surpasses according to interferometry
Accurate three-dimensional measurement.
When carrying out digital hologram ultraprecise three-dimensional measurement, the record of interference fringe and object light is needed to rebuild two steps.
Firstly, use can the longer laser of interference distance as light source, beam of laser is divided into two bundles using optical splitter, due to light splitting
The physical optics characteristic of device, two separated beam laser phase of light wave are believed that identical or phase difference is very small.Secondly, its
Middle beam of laser is not through any object or reflects without object, propagates in space, referred to as reference light；And another beam
Laser light transparent substance is propagated in space after the reflection of opaque article, referred to as object light；Reference light and object
It in body light propagation to another optical splitter and is synthesized, digital camera is irradiated to identical optical axis same propagation direction and is passed
In the same position of sensor.Reference light due to unhinderedly being propagated in space, when being irradiated on digital camera sensor
Phase be believed that with laser issue laser initial phase it is identical, it is believed that reference light phase value be 0；Object light due to
The change of phase occurs by opaque article surface reflection or transparent substance transmission.According to optical interference condition, two column
The direction of propagation is identical, path is identical, wavelength is identical, constant phase difference, the identical light wave in polarization direction can be formed in space it is bright
Interference fringe between dark phase, therefore reference light and object light form interference fringe, light and shade on digital camera sensor surface
Amplitude is in cosine wave (cos), and phase depends on the size of object light and reference light phase difference.Further, digital camera passes
Sensor digitizes interference fringe, and the interference fringe of object light and reference light is recorded in the form of digital picture.Due to number
Word camera sensor only can the strong information of recording light, therefore for the interference fringe of cosine wave (cos) amplitude variations, Wu Fafen
Distinguish object light and reference light phase difference just with it is negative.And the phase difference positive in three-dimensional reconstruction indicates opposite reference plane protrusion
Shape, negative phase difference indicates the shape of opposite reference plane recess, thus in order to accurately calculate phase difference just with it is negative,
Digital Fresnel Diffraction Transform must be used to carry out mathematical computations to interference fringe, then filtered by the band logical in fresnel transform domain
Wave obtains the phase difference for correctly having symbol.Finally, phase difference variation 2pi (i.e. 360 °) expression is produced with reference plane
The range difference (optical path difference) of one optical wavelength, here it is interferometries, using interferometric method, when phase difference variation is more than
It is then needed when 2pi using phase connection method, this makes it possible to realize opaque article surface shape or transparent substance shape
Three-dimensional reconstruction.Since the wavelength unit of visible light is nanometer, it is super that digital hologram three-dimensional measurement can be realized nanoscale
Precision three-dimensional survey.
Currently, digital hologram three-dimensional microscope is as state-of-the-art ultraprecise three-dimensional measurement microscopy apparatus in the world, it is more next
It is utilized in the fields such as preclinical medicine, life science and accurate device detection more.But existing digital hologram three
Dimension microscope uses one-wavelength laser as light source, but cannot achieve and acquires photo chromic microimage when carrying out three-dimensional measurement, and will
Texture of the photo chromic microimage as three-dimensional reconstruction mathematical model, shows together with three-dimensional information.Due to being only able to display artwork master
Picture or the pseudo color image generated according to elevation information, digital hologram three-dimensional microscope is without light such as image of Buddha phase contrast microscopes
Microscope provides intuitive observation like that for observer and looks for multimedia message, so that it is microscopical to limit digital hologram three-dimensional
Application range.
Summary of the invention
To solve the above-mentioned problems, the present invention provides a kind of digital hologram three-dimensional microscopic system and colored micrographs
As the acquisition methods of texture, which is provided with color picture light source, and digital hologram three-dimensional measurement uses monochromatic source, color texture
Monochromatic source is closed when Image Acquisition, using color picture light source, two light sources are switched at high speed, and digital camera high speed acquisition interferes item
Line and color texture image realize the ultraprecise digital hologram three-dimensional measurement for having photo chromic microimage texture of high speed.
In order to achieve the above object, the technical solution adopted by the present invention are as follows:
A kind of digital hologram three-dimensional microscopic system, the monochromatic source including that can emit linearly polarized light can be by the monochromatic light
Source emits beam the spectrophotometric unit for setting up separately and being formed as reference light Yu object light two-beam, and can be photosynthetic with object by reference light
And light combination unit, the other side of the light combination unit is provided with the digital phase that can acquire interference fringe or color texture image
Machine sensor is formed with referring to optical transport optical path and object optical transport light between the spectrophotometric unit and the light combination unit
Road, the starting point of the object optical transport optical path are provided with the color picture light source that can start when acquiring color texture image；Starting
When monochromatic source, color picture light source is closed, so that digital camera sensor acquires interference fringe image；When starting color picture light source, close
Monochromatic source is closed, so that digital camera sensor acquires color texture image.
It is advanced optimized as of the invention, the object optical transport optical path includes first unpolarized point be sequentially arranged
Light device, condenser, transparent objects object, micro objective, the second unpolarized optical splitter and imaging len, described first is non-inclined
Vibration optical splitter and the described second unpolarized optical splitter are symmetrically set in transparent objects object two sides, the first unpolarized light splitting
Device is respectively arranged at two vertical beam projecting ends of the spectrophotometric unit with the light combination unit.
It is advanced optimized as of the invention, the object optical transport optical path includes opaque object, the object light
It is reflected after opaque object.
It is advanced optimized as of the invention, the monochromatic source and the color picture light source are electrically connected with controller, institute
Stating controller includes the acquisition module that can acquire input instruction, and can convert acquisition signal to the control mould of control instruction
Block, the acquisition module are electrically connected the control module, and the control module is connected to the monochromatic source and described
Color picture light source, to control the switch of monochromatic source and color picture light source.
It is advanced optimized as of the invention, the spectrophotometric unit and the light combination unit are polarizing beam splitter.
It is advanced optimized as of the invention, 1/2 wavelength is provided between the monochromatic source and the spectrophotometric unit
Plate, to issue the linearly polarized light with certain angle of polarization.
It is advanced optimized as of the invention, the color picture light source is LED microscope light source.
A kind of acquisition methods of photo chromic microimage texture, utilize digital hologram three-dimensional described in any of the above-described embodiment
The monochromatic source of microscopic system, control digital hologram three-dimensional microscopic system mutually switches with color picture light source, senses digital camera
Device acquires interference fringe and color texture image, to realize the digital hologram three-dimensional measurement for having photo chromic microimage texture.
Advanced optimize as of the invention, comprising the following steps: S0: controller controls monochromatic source and opens, and closes
Color picture light source, the interference fringe of digital camera sensor shooting at this time object light and reference light, and start the phase shift of single exposure
Digital hologram three-dimensional survey calculation；S1: controller control monochromatic source closes and opens color picture light source, digital camera sensor
Photo chromic microimage is shot, digital hologram three-dimensional survey calculation is waited to complete；S2: digital hologram three-dimensional survey calculation is completed, and
The mathematical model for generating three-dimensional reconstruction is added three-dimensional digital model for collected photo chromic microimage as texture, shows simultaneously
It is shown on controller, then observer sees the three-dimensional shape of testee, while observing that the colour of testee is micro-
Information.
It is advanced optimized as of the invention, in step sl, specifically: controller controls monochromatic source and closes, and beats
Open color picture light source, the light of color picture light source transmitting by the first unpolarized optical splitter and collector lens, through transparent objects object or
Opaque object is irradiated into micro objective, and switching digital camera sensor is color sensor, and object light passes through imaging
Lens are imaged on digital camera sensor, to shoot color 2 D micro-image.
Compared with prior art, the advantages and positive effects of the present invention are: the present invention is provided with color picture light source, number
Hologram three-dimensional measurement uses monochromatic source, and color texture image closes monochromatic source when acquiring, uses color picture light source, two light
Source switches at high speed, and digital camera high speed acquisition interference fringe and color texture image, realize high speed has photo chromic microimage
The ultraprecise digital hologram three-dimensional of texture measures.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, below will to embodiment or
Attached drawing needed to be used in the description of the prior art is briefly described, it should be apparent that, the accompanying drawings in the following description is only
Some embodiments of the present invention, for those of ordinary skill in the art, without creative efforts, also
Other drawings may be obtained according to these drawings without any creative labor.
Fig. 1 is the schematic diagram that monochromatic source is opened under the first embodiment of digital hologram three-dimensional microscopic system of the present invention；
Fig. 2 is the schematic diagram that color picture light source is opened under the first embodiment of digital hologram three-dimensional microscopic system of the present invention；
Fig. 3 is the schematic diagram that monochromatic source is opened under second of embodiment of digital hologram three-dimensional microscopic system of the present invention；
Fig. 4 is the schematic diagram that color picture light source is opened under second of embodiment of digital hologram three-dimensional microscopic system of the present invention；
Fig. 5 is the internal module connection figure of controller in digital hologram three-dimensional microscopic system of the present invention.
In above each figure: 1, monochromatic source；2, spectrophotometric unit；3, light combination unit；4, digital camera sensor；41, it dims
Unit；42, polarization plates；5, referring to optical transport optical path；51, the first unpolarized optical splitter；52, condenser；53, transparent objects object；
54, micro objective；55, the second unpolarized optical splitter；56, imaging len；57, opaque object；6, object optical transport light
Road；7, color picture light source；8, controller；81, acquisition module；82, control module；9,1/2 wavelength plate；10, plane mirror；11,
1/4 wavelength plate.
Specific embodiment
In the following, the present invention is specifically described by illustrative embodiment.It should be appreciated, however, that not into
In the case that one step describes, element, structure and features in an embodiment can also be advantageously incorporated into other embodiment party
In formula.
In the description of the present invention, it is to be understood that, term " center ", "upper", "lower", "front", "rear", " left side ",
The orientation or positional relationship of the instructions such as " right side ", "vertical", "horizontal", "top", "bottom", "inner", "outside" is based on the figure
Orientation or positional relationship is merely for convenience of description of the present invention and simplification of the description, rather than the device of indication or suggestion meaning or
Element must have a particular orientation, be constructed and operated in a specific orientation, therefore be not considered as limiting the invention.
Term " first ", " second " be used for descriptive purposes only and cannot be understood as indicating or suggesting relative importance or
Implicitly indicate the quantity of indicated technical characteristic." first " is defined as a result, the feature of " second " can be expressed or hidden
It include one or more of the features containing ground.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " installation ",
" connected ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or integrally connect
It connects；It can be directly connected, the connection inside two elements can also be can be indirectly connected through an intermediary.For this
For the those of ordinary skill in field, the concrete meaning of above-mentioned term in the present invention can be understood with concrete condition.
Shown in referring to figures 1-4, the present invention provides a kind of digital hologram three-dimensional microscopic system, the digital hologram three-dimensionals
Microscopic system includes that can emit the monochromatic source 1 of linearly polarized light, and the monochromatic source 1, which can emit beam to set up separately, is formed as joining
The spectrophotometric unit 2 of irradiation and object light two-beam, and the light combination unit 3 that reference light can be merged with object light, the light combination
The other side of unit 3 is provided with the digital camera sensor 4 that can acquire interference fringe or color texture image, and the light splitting is single
It is formed between member 2 and the light combination unit 3 referring to optical transport optical path 5 and object optical transport optical path 6, the object light passes
The starting point for losing road 5 is provided with the color picture light source 7 that can start when acquiring color texture image；When starting monochromatic source, close
Color picture light source is closed, so that digital camera sensor acquires interference fringe image；When starting color picture light source, monochromatic source is closed, with
Digital camera sensor is set to acquire color texture image.
Technical solution of the present invention uses monochromatic source in digital hologram three-dimensional measurement by adding color picture light source,
Color texture image closes monochromatic source when acquiring, and using color picture light source, two light sources are switched at high speed, and digital camera high speed is adopted
Collect interference fringe and color texture image, and then the ultraprecise number for having photo chromic microimage texture for realizing high speed is complete
Cease three-dimensional measurement.
As shown in Figure 1, the object optical transport optical path 5 includes the first unpolarized optical splitter 51, the condenser being sequentially arranged
52, transparent objects object 53, micro objective 54, the second unpolarized optical splitter 55 and imaging len 56, described first is unpolarized
Optical splitter 51 and the described second unpolarized optical splitter 55 are symmetrically set in 53 two sides of transparent objects object, and described first is unpolarized
Optical splitter 51 is respectively arranged at two vertical beam projecting ends of the spectrophotometric unit 2 with the light combination unit 3.
Under this kind of embodiment, when carrying out three-dimensional measurement, color picture light source is closed, opens monochromatic source, monochromatic source hair
Light out carries out the adjustment of polarization direction by 1/2 wavelength plate, is divided into two bundles by spectrophotometric unit, a branch of horizontal polarization light
It is reflected by the first unpolarized optical splitter, it is poly- by condenser, through the transparent objects object such as cell, focus on microscope object
In the focus of mirror, because object lens multiplying power more big imaging light intensity is weaker, so can guarantee high magnification object lens so also and can have and is brighter
Imaging.The light of micro objective output is reflected by the first unpolarized optical splitter, forms horizontal polarization object light, and
By imaging len, it is imaged on digital camera sensor；Another beam orthogonal polarized light is directly propagated in space, at
It for vertical polarization reference light, and is synthesized with horizontal polarization object light by light combination unit, becomes propagation side in same optical axis
To identical, wavelength is identical, the light of constant phase difference, but at this time since polarization direction is orthogonal thereto, reference light and object light
It does not interfere.Reference light and object light become the opposite circularly polarized light in direction of rotation by 1/4 wavelength plate, pass through polarization plates
It is identical to become the direction of propagation, optical axis is identical, and wavelength is identical, and polarization direction is identical, the light of constant phase difference, and in digital camera
It is interfered on sensor.Digital camera shoots interference fringe, is carried out using single exposure digital hologram three-dimensional mensuration real-time
Three-dimensional measurement.
When carrying out two-dimensional color micro-image texture collection, monochromatic source is closed, opens color picture light source, color picture light source
The light of sending is irradiated into microscope object through the transparent objects object such as cell by the first unpolarized optical splitter and collector lens
Mirror, and be imaged on digital camera sensor by imaging len.At this time digital camera sensor need to use color sensor with
Shoot color 2 D micro-image.Since color picture light source has certain light intensity attenuation through multiple optical devices, preferably
The highlighted LED using 3-10W power as microscope light source.
Among the above, it is preferred that the color picture light source 7 is LED microscope light source.
As shown in figure 3, the object optical transport optical path includes opaque object 57, the object light is by opaque
It is reflected after object.In addition, successively being set after spectrophotometric unit 2 on the reference light pipeline 6 under this kind of embodiment
It is equipped with 1/4 wavelength plate 11 and plane mirror 10.
Under this kind of embodiment, light path principle under the embodiment of transparent objects object principle it is consistent, it is no longer superfluous herein
It states.Only need micro-adjustment, under embodiment shown in Fig. 3, the light in reference light optical path passes through 1/4 wavelength plate 11
Afterwards by being reflected again after plane mirror 10.
Above scheme shown in Figure 5, of the invention, monochromatic source 1 described further and the color picture light source 7 are electrical
It is connected with controller 8, the controller 8 includes the acquisition module 81 that can acquire input instruction, and can be by acquisition signal conversion
For the control module 82 of control instruction, the acquisition module 81 is electrically connected the control module 82, and the control module 82 is divided
It is not connected to the monochromatic source 1 and the color picture light source 7, to control the switch of monochromatic source and color picture light source.
Preferably, among the above, the spectrophotometric unit 2 and the light combination unit 3 are polarizing beam splitter.And the monochrome
1/2 wavelength plate 9 is provided between light source 1 and the spectrophotometric unit 2, to issue the linearly polarized light with certain angle of polarization.
In addition, it is provided with polarized geophone between light combination unit 3 and digital camera sensor 4 in the present invention, the polarization
Wave detector is made of dimming unit 41 and polarization plates 42, the vertical polarization reference light and horizontal polarization object projected from light combination unit 4
Body light passes through dimming unit 41 first, becomes circularly polarized light from linearly polarized light, at this point, when entering dimming unit due to the two
Polarization direction is different, and the initial rotation of circular polarization is contrary when through dimming unit 41, therefore circle is inclined when propagating in space
The Sense of polarization of reference light of shaking and circular polarization object light is not on the contrary, simultaneously as therefore the two polarization direction is on the contrary, send out
Raw interference phenomenon.Circular polarization reference light and circular polarization object light, which penetrate polarization plates 42, becomes linear polarization reference light and linear polarization
Object light is traveled to identical polarization direction in the plane of digital camera sensor 4, at this point, due to the two polarization direction phase
Together, constant phase difference, wavelength is identical, and the direction of propagation is identical, therefore interferes phenomenon.Because of surface optical device multiple reflections
Etc. optical noises polarization direction it is not identical as incident reference light and object light, therefore can not be in digital camera sensor 4
It is upper to interfere phenomenon together with reference light and object light, it is small with high accuracy that noise can be obtained by optical method with this
Clear interference fringe solves the problems, such as that signal-to-noise ratio is small in ordinary optical interference, provides for precise figures hologram three-dimensional measurement
High-quality basis.
On the other hand, the polarization plates 42 in polarized geophone have locking circular polarization reference light and circular polarization object light phase
The ability of potential difference increases additional setting phase except the object light and reference light phase difference generated due to object itself
Difference realizes the generation of phase shift interference striped.
In addition, being implemented the invention also provides a kind of acquisition methods of photo chromic microimage texture using any of the above-described kind
Example described in digital hologram three-dimensional microscopic system, method particularly includes: control digital hologram three-dimensional microscopic system monochromatic source with
Color picture light source mutually switches, and makes digital camera sensor acquisition interference fringe and color texture image, has colored show to realize
The digital hologram three-dimensional of micro- image texture measures.
Further refinement, the acquisition methods of photo chromic microimage texture of the present invention, specifically includes the following steps:
S0: controller controls monochromatic source and opens, and closes color picture light source, and digital camera sensor shoots object at this time
The interference fringe of light and reference light, and start the phase-shifted digital hologram three-dimensional survey calculation of single exposure；
S1: color picture light source is closed and opened to controller control monochromatic source, and digital camera sensor shoots colored micrograph
Picture waits digital hologram three-dimensional survey calculation to complete；
S2: digital hologram three-dimensional survey calculation is completed, and generates the mathematical model of three-dimensional reconstruction, by collected colour
Three-dimensional digital model is added as texture in micro-image, while being shown on controller, then observer sees the three of testee
Three-dimensional shape is tieed up, while observing the colored microscopy information of testee.
In addition, in step sl, specifically: controller controls monochromatic source and closes, and opens color picture light source, color picture light
The light of source transmitting is by the first unpolarized optical splitter and collector lens, through transparent objects object or opaque object, irradiation
Enter micro objective, switching digital camera sensor is color sensor, and object light is sensed by imaging len in digital camera
It is imaged on device, to shoot color 2 D micro-image.
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, appoints
What those familiar with the art in the technical scope disclosed by the present invention, can easily think of the change or the replacement, answer
It is included within the scope of the present invention.Therefore, protection scope of the present invention should be with the scope of protection of the claims
It is quasi-.
Claims (10)
1. a kind of digital hologram three-dimensional microscopic system, the monochromatic source including that can emit linearly polarized light can be by the monochromatic source
Emit beam the spectrophotometric unit for setting up separately and being formed as reference light Yu object light two-beam, and reference light can be merged with object light
Light combination unit, the other side of the light combination unit are provided with the digital camera sensing that can acquire interference fringe or color texture image
Device, it is characterised in that: be formed between the spectrophotometric unit and the light combination unit and passed referring to optical transport optical path and object light
Road is lost, the starting point of the object optical transport optical path is provided with the color picture light source that can start when acquiring color texture image；
When starting monochromatic source, color picture light source is closed, so that digital camera sensor acquires interference fringe image；Start color picture light source
When, monochromatic source is closed, so that digital camera sensor acquires color texture image.
2. digital hologram three-dimensional microscopic system according to claim 1, it is characterised in that: the object optical transport optical path includes
The unpolarized optical splitter of first be sequentially arranged, condenser, transparent objects object, micro objective, the second unpolarized optical splitter and
Imaging len, the first unpolarized optical splitter and the described second unpolarized optical splitter are symmetrically set in the transparent objects object two
Side, the first unpolarized optical splitter go out with two vertical light that the light combination unit is respectively arranged at the spectrophotometric unit
Penetrate end.
3. digital hologram three-dimensional microscopic system according to claim 1, it is characterised in that: the object optical transport optical path includes
Opaque object, the object light reflect after opaque object.
4. digital hologram three-dimensional microscopic system described in any one of -3 according to claim 1, it is characterised in that: the monochromatic source
It is electrically connected with controller with the color picture light source, the controller includes the acquisition module that can acquire input instruction, Yi Jike
Convert acquisition signal to the control module of control instruction, the acquisition module is electrically connected the control module, the control
Module is connected to the monochromatic source and the color picture light source, to control the switch of monochromatic source and color picture light source.
5. digital hologram three-dimensional microscopic system described in any one of -3 according to claim 1, it is characterised in that: the spectrophotometric unit
It is polarizing beam splitter with the light combination unit.
6. digital hologram three-dimensional microscopic system described in any one of -3 according to claim 1, it is characterised in that: the monochromatic source
It is provided with 1/2 wavelength plate, between the spectrophotometric unit to issue the linearly polarized light with certain angle of polarization.
7. digital hologram three-dimensional microscopic system according to claim 1, it is characterised in that: the color picture light source is that LED is micro-
Mirror light source.
8. a kind of acquisition methods of photo chromic microimage texture, utilize digital hologram three-dimensional described in any one of claim 1-7
Microscopic system, it is characterised in that: the monochromatic source of control digital hologram three-dimensional microscopic system mutually switches with color picture light source, makes to count
Word camera sensor acquires interference fringe and color texture image, to realize the digital hologram three for having photo chromic microimage texture
Dimension measurement.
9. the acquisition methods of photo chromic microimage texture according to claim 8, it is characterised in that: the following steps are included:
S0: controller controls monochromatic source and opens, and closes color picture light source, at this time digital camera sensor shooting object light and ginseng
The interference fringe of irradiation, and start the phase-shifted digital hologram three-dimensional survey calculation of single exposure；
S1: color picture light source is closed and opened to controller control monochromatic source, and digital camera sensor shoots photo chromic microimage, etc.
It is completed to digital hologram three-dimensional survey calculation；
S2: digital hologram three-dimensional survey calculation is completed, and generates the mathematical model of three-dimensional reconstruction, by collected colored micrograph
It as three-dimensional digital model is added as texture, while being shown on controller, then observer sees the 3 D stereo of testee
Shape, while observing the colored microscopy information of testee.
10. the acquisition methods of photo chromic microimage texture according to claim 9, it is characterised in that: in step sl, specifically
Are as follows: controller controls monochromatic source and closes, and opens color picture light source, and the light of color picture light source transmitting passes through the first unpolarized light splitting
Device and collector lens are irradiated into micro objective through transparent objects object or opaque object, switch digital camera sensor
For color sensor, object light is imaged on digital camera sensor by imaging len, to shoot color 2 D micro-image.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711237135.XA CN109855529A (en) | 2017-11-30 | 2017-11-30 | The acquisition methods of digital hologram three-dimensional microscopic system and photo chromic microimage texture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711237135.XA CN109855529A (en) | 2017-11-30 | 2017-11-30 | The acquisition methods of digital hologram three-dimensional microscopic system and photo chromic microimage texture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109855529A true CN109855529A (en) | 2019-06-07 |
Family
ID=66887923
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711237135.XA Pending CN109855529A (en) | 2017-11-30 | 2017-11-30 | The acquisition methods of digital hologram three-dimensional microscopic system and photo chromic microimage texture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109855529A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112013791A (en) * | 2020-09-02 | 2020-12-01 | 常州机电职业技术学院 | Three-wavelength variable-scale interference microscopic imaging system, imaging method and three-phase restoration method |
| CN112596362A (en) * | 2020-12-18 | 2021-04-02 | 昆明理工大学 | Full-field super-resolution digital holographic device and imaging method |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101087991A (en) * | 2004-12-22 | 2007-12-12 | 国立大学法人电气通信大学 | Three-dimensional shape measuring instrument |
| JP2011034023A (en) * | 2009-08-06 | 2011-02-17 | Sony Corp | Three-dimensional image reproducing display device, three-dimensional image reproducing display method, three-dimensional image information acquisition reproducing system, and three-dimensional image information acquisition reproducing method |
| CN204388780U (en) * | 2014-12-10 | 2015-06-10 | 佛山市南海区欧谱曼迪科技有限责任公司 | A kind of multi-wavelength phase shift micro imaging system |
| CN105241374A (en) * | 2015-10-16 | 2016-01-13 | 哈尔滨工程大学 | Dual wavelength common-channel quadrature carrier frequency digital holographic detection apparatus and detection method |
| KR20160029606A (en) * | 2014-09-05 | 2016-03-15 | 광운대학교 산학협력단 | Digital holographic microscopy and method for generating digital holographic image |
| CN207675118U (en) * | 2017-11-30 | 2018-07-31 | 青岛全维医疗科技有限公司 | Digital hologram three-dimensional microscopic system |
-
2017
- 2017-11-30 CN CN201711237135.XA patent/CN109855529A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101087991A (en) * | 2004-12-22 | 2007-12-12 | 国立大学法人电气通信大学 | Three-dimensional shape measuring instrument |
| JP2011034023A (en) * | 2009-08-06 | 2011-02-17 | Sony Corp | Three-dimensional image reproducing display device, three-dimensional image reproducing display method, three-dimensional image information acquisition reproducing system, and three-dimensional image information acquisition reproducing method |
| KR20160029606A (en) * | 2014-09-05 | 2016-03-15 | 광운대학교 산학협력단 | Digital holographic microscopy and method for generating digital holographic image |
| CN204388780U (en) * | 2014-12-10 | 2015-06-10 | 佛山市南海区欧谱曼迪科技有限责任公司 | A kind of multi-wavelength phase shift micro imaging system |
| CN105241374A (en) * | 2015-10-16 | 2016-01-13 | 哈尔滨工程大学 | Dual wavelength common-channel quadrature carrier frequency digital holographic detection apparatus and detection method |
| CN207675118U (en) * | 2017-11-30 | 2018-07-31 | 青岛全维医疗科技有限公司 | Digital hologram three-dimensional microscopic system |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112013791A (en) * | 2020-09-02 | 2020-12-01 | 常州机电职业技术学院 | Three-wavelength variable-scale interference microscopic imaging system, imaging method and three-phase restoration method |
| CN112596362A (en) * | 2020-12-18 | 2021-04-02 | 昆明理工大学 | Full-field super-resolution digital holographic device and imaging method |
| CN112596362B (en) * | 2020-12-18 | 2022-07-26 | 昆明理工大学 | Digital holographic device and imaging method for full-field super-resolution |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN207675118U (en) | Digital hologram three-dimensional microscopic system | |
| US11680790B2 (en) | Multiple channel locating | |
| KR101441245B1 (en) | Digital Holographic Microscope Apparatus | |
| JP6716121B2 (en) | Digital holographic microscope | |
| US10712270B2 (en) | Digital holographic microscope | |
| CN107490562B (en) | Ultra-high-speed three-dimensional refractive index image shooting and fluorescent structured light illuminating microscope system using wave surface shaper and using method thereof | |
| CN106292238B (en) | A kind of reflective off-axis digital holography micro-measurement apparatus | |
| JP2020516949A (en) | Skew mirror auxiliary imaging | |
| CN108415230A (en) | A kind of novel changable coke digital holographic microscope | |
| CN103733144A (en) | Method for optical tomography | |
| US9256204B2 (en) | Device and method for holographic reflection imaging | |
| CN106767534B (en) | Stereomicroscopy system and mating 3 d shape high score reconstructing method based on FPM | |
| CN108168425A (en) | A kind of Digital holographic microscopy system with novel time-lapse system | |
| KR20190099859A (en) | Self-Interference Digital Holographic System | |
| Yang et al. | Optical MEMS devices for compact 3D surface imaging cameras | |
| CN109855529A (en) | The acquisition methods of digital hologram three-dimensional microscopic system and photo chromic microimage texture | |
| CN208297941U (en) | A kind of novel changable coke digital holographic microscope | |
| CN111610150A (en) | Full-field structured light coherent coding tomography device and method | |
| CN207676126U (en) | Phase shift interference striped generates system | |
| CN104793475B (en) | A kind of incoherent digital hologram imaging method and corollary apparatus of looking in the distance | |
| CN108088368A (en) | Reflective off-axis digital holography apparatus and method based on light splitting pupil | |
| CN109496262A (en) | Whole audience interference imaging system and whole audience interference imaging method | |
| CN109856943B (en) | Phase shift interference fringe generation system and generation method thereof | |
| KR101078197B1 (en) | Polarized point-diffraction interferometer for aligning optical system | |
| CN112945083A (en) | Parallel phase shift digital holographic microscopic imaging system with optical fiber interconnection |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |