CN109846775A - A kind of Abelmoschus esculentus fruit Essence and preparation method thereof - Google Patents

Abstract

The present invention provides a kind of Abelmoschus esculentus fruit Essences and preparation method thereof, belong to cosmetic field, the Essence includes Abelmoschus esculentus fruit extracting solution 70%~84%, glycerol 5%~15%, propylene glycol 10%~20%, natural antibacterial agent 0.01%~0.1%, natural 0.05%~0.5%, the Essence has quickly potent moisturizing moisturizing, firm skin, anti-oxidant, anti-inflammatory efficacy, it is environmental-friendly, using pure natural plant extracts as preservative antioxidant, safety and stability, moreover it is possible to increase moisturizing moisturizing and other effects.Wherein Abelmoschus esculentus fruit extracting solution is to be extracted using Abelmoschus esculentus fruit as raw material by water at low temperature, filtering, is obtained after high speed centrifugation；Again with glycerol, propylene glycol, natural antibacterial agent, natural compounded to obtain Abelmoschus esculentus fruit Essence, simple production process, and high financial profit is suitble to industrialization production.

Description

A kind of Abelmoschus esculentus fruit Essence and preparation method thereof

Technical field

The invention belongs to moisturizing moisturizing cosmetic technical field, specifically a kind of Abelmoschus esculentus fruit Essence and preparation side Method.

Background technique

Cosmetic industry is in the rapid development stage, and product makes rapid progress, and type emerges one after another, Various Functions, this is to wide Big consumer brings certain puzzlement, generally requires to buy a variety of cosmetics and is used cooperatively, to spend mint of money and time It is long.The safety issue of cosmetics is also increasingly taken seriously simultaneously, and pure natural safely and effectively cosmetics is selected to have become A kind of trend.

Moisture is the basis of skin, and keeping the moisture of skin is the key that solve the problems, such as skin, and skin water shortage will lead to secretly It is light, the problems such as microgroove, the scales of skin that peel off.The yellow ashamed fruit of coffee is the common fruit from Perenniporia martius, rich in viscous polysaccharide and micro Element can quickly be absorbed by the skin and form special protective film on surface, effectively prevent water loss while keeping the skin wet, make Skin keeps moisture expanded state, and referred to as " plant sodium hyaluronate ", but the cosmetics of Abelmoschus esculentus berry extract are added in market It is not efficiently use its moisturizing moisturizing and other effects as a kind of antioxidant.

Natural antiseptic agent, antibacterial agent and antioxidant are the hot spots that cosmetic industry is studied at present.Natural antibacterial agent be by Perhaps the existing substance with bacteriostasis is manually extracted or is process as antibacterial agent in vivo for organism secretion, this Class antibacterial agent is natural materials, therefore harmless to the human body；Capillary extract, Magnolia cortex P.E, shrubby sophora extract, Chinese gall are extracted Object is all the known substance with outstanding antiseptic and inhibiting bacteria function effect, is the optimal selection of natural antibacterial agent.Vitamin C, dimension life Plain E, tea polyphenols and Rosmarinus officinalis extract all have stronger inoxidizability, and will not generate harm to human body, and wherein tea is more Phenol and Rosmarinus officinalis extract are all naturals, and wherein vitamin C, vitamin E and Rosmarinus officinalis extract are gone back by compounding Can reach similar TBHQ (tert-butyl hydroquinone has a kind of extremely light peat-reek for white or micro- bronzing crystalline powder, It is practically insoluble in water, is dissolved in the organic solvents such as ethyl alcohol, ethyl acetate, ether.It is a kind of powerful antioxidant, free radical can be removed) The effect of effect.

We devise using Abelmoschus esculentus fruit extracting solution as raw material thus, and natural antiseptic agent and antioxidant is added, and guarantee The pure natural characteristic and stability of product, moreover it is possible to increase moisturizing moisturizing, firm skin desalinates microgroove, anti-oxidant anti-inflammatory effect.

Summary of the invention

In view of the above shortcomings of the prior art, the technical problem to be solved in the present invention is to provide one kind have moisturizing moisturizing, Firm skin, desalination microgroove, Abelmoschus esculentus fruit Essence of anti-oxidant anti-inflammatory efficacy and preparation method thereof.

In order to solve the above technical problems, the present invention provides the following technical scheme that

A kind of Abelmoschus esculentus fruit Essence, comprises the following raw materials by weight percent:

Abelmoschus esculentus fruit extracting solution 70%~85%

Glycerol 5%~15%

Propylene glycol 8%~20%

Natural antibacterial agent 0.01%~0.1%

Natural 0.05%~0.5%.

Wherein, Abelmoschus esculentus fruit extracting solution is the water extract of Abelmoschus esculentus fruit, the preparation method comprises the following steps: weighing fresh coffee Huang Certain herbaceous plants with big flowers fruit cleans impurity, is sliced 2~4mm sheet, puts into extraction element, and 1~3 times of distillation flooding 60min is first added, and extracts Temperature is 40~50 DEG C, and with filtered through gauze, filtrate is abandoned；The distillation flooding that 2~4 times of amounts are added in filter residue continues to extract 60min, Extracting temperature are 40~50 DEG C, with filtered through gauze, slightly extruding filter residue, flow out extracting solution sufficiently；It is obtained above-mentioned Extracting solution be centrifuged, revolving speed is 8000~10000r/min, and centrifugation time is 5~10min, and obtaining supernatant is that coffee is yellow Certain herbaceous plants with big flowers fruit extracting solution；

Wherein, the natural antibacterial agent is by Magnolia cortex P.E, capillary extract, shrubby sophora extract, nutgall extractive, snake One of machine tool extract or a variety of compositions；

Wherein, the natural is by natural vitamin C, natural VE, Rosmarinus officinalis extract, in tea polyphenols One or more compositions.

For above-mentioned formula, it is preferable that comprise the following raw materials by weight percent:

Abelmoschus esculentus fruit extracting solution 74.78%

Glycerol 9.97%

Propylene glycol 14.95%

Natural antibacterial agent 0.05%

Natural 0.25%.

The present invention also provides the preparation methods of above-mentioned Abelmoschus esculentus fruit Essence, specifically comprise the following steps:

Step 1: weighing a certain amount of fresh coffee ambrette fruit, impurity is cleaned, is sliced 2~4mm sheet, investment extracts dress In setting, 1~3 times of distillation flooding 60min is first added, Extracting temperature, which is 20~60 DEG C, (in the temperature range, will not destroy and mention The activity of liquid ingredient is taken, Extracting temperature is suitably adjusted according to Abelmoschus esculentus fruit weight is added, and additional amount mostly appropriate increase mentions Take temperature), with filtered through gauze, filtrate is abandoned；The distillation flooding that 2~4 times of amounts are added in filter residue continues to extract 60min, mentions Taking temperature to be 20~60 DEG C (in the temperature range, will not destroy the activity of extracting solution ingredient, Extracting temperature is according to addition coffee Ambrette fruit weight is suitably adjusted, and additional amount is mostly appropriate to increase Extracting temperature), with filtered through gauze, slightly extruding filter residue, make to mention Liquid is taken sufficiently to flow out；

Step 2: extracting solution obtained above is centrifuged, revolving speed is 8000~10000r/min, centrifugation time 5 ~10min, supernatant are Abelmoschus esculentus fruit extracting solution；

Step 3: the preparation of A liquid: weighing the glycerol of formula ratio, Abelmoschus esculentus fruit extracting solution and glycerol are carried out sufficiently mixed It closes and uniformly obtains A liquid；

Step 4: the preparation of B liquid: propylene glycol, the natural antibacterial agent, natural of formula ratio are weighed respectively, in temperature Under the conditions of 50 DEG C of degree, three is stirred dissolution, obtains clear liquid, which is B liquid；

Step 5: A liquid and B liquid are stirred and evenly mixed by high-speed shearing machine, Abelmoschus esculentus fruit Essence is obtained.

Abelmoschus esculentus nutritive value is abundant, and a large amount of stick-slip juice is contained in Abelmoschus esculentus fruit, has special fragrance, Pectin, cow's milk glycan and my glycan etc. are mixed in juice.Its pectin is Soluble Fiber, the pole in the new idea of modern health To pay attention to, according to surveying and determination containing -2.5 grams of protein, fatty -0.1 gram, -2.7 grams of carbohydrate in every hectogram okra fruit, cellulose A-660 is international Unit, -0.2 milligram of vitamin B1, -0.06 milligram of vitamin B2, -44 milligrams of vitamin, -81 milligrams of calcium, -63 milligrams of phosphorus, iron - 0.8 milligram, be a kind of new century ideal high-grade green nourishing health-care vegetable.These viscosity being rich in Abelmoschus esculentus fruit are more Sugar, pectin, protein and microelement can quickly be absorbed by the skin and form special protective film on surface, effectively prevent water It shunts and loses while keeping the skin wet, skin is made to keep moisture expanded state, and moisture is the basis of skin, and the moisture of skin is kept to be Solve the problems, such as the key of skin, skin water shortage will lead to dim, inflammation, the problems such as generating microgroove, the scales of skin that peel off.

Glycerol is widely used in fields such as food, medicine, cosmetics, to produce various preparations, solvent, hygroscopic agent, Antifreezing agent and sweetener, ingredients externally-applied ointment or suppository etc. also have high activity, antioxygen in addition to having the function of moisturizing, humectation Change, promote the special efficacies such as alcoholization.Due to containing abundant viscous polysaccharide, pectin, protein and micro member in above-mentioned Abelmoschus esculentus fruit Element can effectively be fused using glycerol as solvent.

The weak acid environment that our skins are liked, the also exactly habitat of microbial growth, microorganism be easy Mass propagation is grown in cosmetics, can be absorbed, decomposes and be destroyed the effective component of kind of making up, lead to cosmetics from deterioration, mouldy And corruption, and it is rotten when the product that decomposes stimulation can be generated to skin, the pathogen of breeding can also cause human body diseases, institute With antibacterial agent just at essential ingredient in cosmetics.Thorn can be generated to skin by extracting the antibacterial agent produced by chemical industry Swash effect, and natural antibacterial agent is milder, it is harmless to the human body.Capillary extract, Magnolia cortex P.E, shrubby sophora extract, Nutgall extractive is all the known substance with outstanding antiseptic and inhibiting bacteria function effect, is the optimal selection of natural antibacterial agent, and It is from a wealth of sources, it is at low cost.

Oxidation is the biggest threat of skin aging, and diet is unhealthy, solarization, pressure, environmental pollution etc. can allow skin from It is spread unchecked by base, to generate the oxidative phenomenas such as complexion dimness, water shortage, anti-oxidant skin care item can make skin anti-aging that may delay Physical efficiency recovery occurs and accelerates for exercise induced fatigue.Antioxidant is added in cosmetics can also prevent the ingredient in cosmetics from generating Oxidation reaction, which generates, to go bad, and causes damages to skin.Vitamin C, vitamin E, tea polyphenols and Rosmarinus officinalis extract all have compared with Strong inoxidizability, and harm will not be generated to human body, vitamin C, vitamin E are human essential elements, tea polyphenols and fan Repeatedly fragrant extract belongs to natural plant extracts, is the known natural raw material with good antioxidant effect.

Propylene glycol is standing to be used as in cosmetics, toothpaste and perfumed soap and can be used as wetting agent with glycerol or sorbierite cooperation, is being cured It is used as the solvent of blender, preservative, ointment, ointment, pill and vitamin etc. in medicine industry, also serves as softening agent and figuration Agent；Since above-mentioned natural antibacterial agent and natural are insoluble in water, organic solvent is dissolved in, using propylene glycol as reconciliation Solvent is simple and easy to get, harmless.

Compared with prior art, the beneficial effects of the present invention are:

1, the Essence has moisturizing moisturizing, and firm skin desalinates microgroove, anti-inflammatory oxidation resistant effect；

2, the effective component in Abelmoschus esculentus fruit is extracted using low temperature, its activity can be protected to greatest extent, and be not related to It is environmental-friendly to the poisonous and hazardous organic solvent of human body, guarantee product the effect of be not affected；

3, using pure natural plant extracts as preservative antioxidant, it both can guarantee that product safety was stablized, moreover it is possible to Increase moisturizing moisturizing and other effects；

4, it uses common Abelmoschus esculentus fruit for raw material, is easy to get, it is economical and practical；

5, preparation process is simple, is suitble to industrialization production；

The Essence is liquid condition, easy to use, is applied to dry skin position, rapid-action, can be quickly by skin Skin absorbs, and achievees the effect that moisturizing moisturizing.

Detailed description of the invention

LOR expression of results is tested-is promoted in Fig. 1 Abelmoschus esculentus fruit Essence moisturizing；

IVL expression of results is tested-is promoted in Fig. 2 Abelmoschus esculentus fruit Essence moisturizing；

The anti-inflammatory experiment of Fig. 3 Abelmoschus esculentus fruit Essence-inhibition TNF-α expression of results；

The anti-inflammatory experiment of Fig. 4 Abelmoschus esculentus fruit Essence-inhibition IL-6 expression of results；

In figure: B: blank group；M: damage group；1/2/3 respectively corresponds embodiment 1/2/3；

LOR: loricrin；IVL: involucrin；TNF-α: tumor necrosis factor；IL-6: interleukin 6.

Specific embodiment

The technical solution of the patent is explained in further detail With reference to embodiment, but the present invention is not only It is only limitted to following embodiment.Within the scope of the present invention or the contents of the present invention, spirit and scope are not being departed from It is interior, improvement, the identical component of replacement effect of non-creative labour are carried out to Abelmoschus esculentus fruit Essence of the present invention, for It will be apparent from for those skilled in the art, they are considered as including within protection scope of the present invention.

A kind of preparation method of the Abelmoschus esculentus fruit Essence of the present invention of embodiment 1.

Step 1: weighing fresh coffee ambrette fruit 200g, the impurity such as silt, dirt are washed with clean water, 2~4mm is cut into Sheet；The Abelmoschus esculentus fruit being sliced is put into extraction element, the distilled water of 400g is added, 20 DEG C of temperature, impregnates 60min Afterwards, with filtered through gauze, filtrate discarding；Filter residue continues to extract；Again plus 400g distilled water is in percolating device, 20 DEG C of temperature, impregnates After 60min, with filtered through gauze, slightly squeezes, flow out extracting solution sufficiently；

Step 2: the extracting solution that previous step is obtained removes impurity, revolving speed 10000r/min, centrifugation by centrifuge Time 5min, takes supernatant, obtains Abelmoschus esculentus fruit extracting solution 326g；

Step 3: preparing A liquid: the glycerol and 300g Abelmoschus esculentus fruit extracting solution that 30g is added mix well, and obtain A liquid 330g；

Step 4: preparing B liquid: the propylene glycol of 50g is weighed, natural antibacterial agent 0.05g, natural 0.5g is added, B liquid 50.55g is obtained after temperature 50 C, stirring and dissolving；

Step 5: stirring and evenly mixing A liquid and B liquid to obtain Abelmoschus esculentus fruit Essence 380.55g by high-speed shearing machine.

Another preparation method of the Abelmoschus esculentus fruit Essence of the present invention of embodiment 2.

Step 1: weighing fresh coffee ambrette fruit 500g, the impurity such as silt, dirt are washed with clean water, 2~4mm is cut into Sheet；The Abelmoschus esculentus fruit being sliced is put into extraction element, the distilled water of 1500g is added, 40 DEG C of temperature, impregnates After 60min, with filtered through gauze, filtrate is abandoned；Filter residue continues to extract；Again plus 750g distilled water is in percolating device, and 40 DEG C of temperature, After impregnating 60min, with filtered through gauze, slightly squeezes, flow out extracting solution sufficiently；

Step 2: the extracting solution that previous step is obtained removes impurity, revolving speed 8000r/min, centrifugation by centrifuge Time 10min, takes supernatant, obtains Abelmoschus esculentus fruit extracting solution 613g；

Step 3: preparing A liquid: the glycerol and 600g Abelmoschus esculentus fruit extracting solution that 80g is added mix well, and obtain A liquid 680g；

Step 4: preparing B liquid: the propylene glycol of 120g is weighed, natural antibacterial agent 0.4g, natural 2g is added, in Temperature 50 C obtains B liquid 122.4g after stirring and dissolving；

Step 5: stirring and evenly mixing A liquid and B liquid to obtain Abelmoschus esculentus fruit Essence 802.4g by high-speed shearing machine.

Another preparation method of the Abelmoschus esculentus fruit Essence of the present invention of embodiment 3.

Step 1: weighing fresh coffee ambrette fruit 1000g, the impurity such as silt, dirt are washed with clean water, 2~4mm is cut into Sheet；The Abelmoschus esculentus fruit being sliced is put into extraction element, the distilled water of 4000g is added, temperature 60 C impregnates After 60min, with filtered through gauze, filtrate is abandoned；Filter residue continues to extract；Again plus 2000g distilled water is in percolating device, temperature 60 DEG C, after impregnating 60min, with filtered through gauze, slightly squeezes, flow out extracting solution sufficiently；

Step 2: the extracting solution that previous step is obtained removes impurity, revolving speed 8000r/min, centrifugation by centrifuge Time 8min, takes supernatant, obtains Abelmoschus esculentus fruit extracting solution 1694g；

Step 3: preparing A liquid: the glycerol and 1500g Abelmoschus esculentus fruit extracting solution that 200g is added mix well, and obtain A liquid 1700g；

Step 4: preparing B liquid: the propylene glycol of 400g is weighed, natural antibacterial agent 1.5g, natural 8.5g is added, B liquid 410g is obtained after temperature 50 C, stirring and dissolving；

Step 5: stirring and evenly mixing A liquid and B liquid to obtain Abelmoschus esculentus fruit Essence 2110g by high-speed shearing machine.

Weight percentage of each component is as shown in table 1 below in three above embodiment:

Component	Embodiment 1	Embodiment 2	Embodiment 3
				Abelmoschus esculentus fruit extracting solution	78.83%	74.78%	71.09%
Glycerol	7.88%	9.97%	9.48%
				Propylene glycol	13.15%	14.95%	18.96%
Natural antibacterial agent	0.01%	0.05%	0.07%
				Natural	0.13%	0.25%	0.40%
It is total	100%	100%	100%

Table 1

Experimental verification one:

By establishing acetone-ether drying model, any one Abelmoschus esculentus fruit essence in above-mentioned case study on implementation is verified Liquid all has moisturizing, moisturizing, firm skin, and anti-oxidant, anti-inflammatory effect, the specific operation method is as follows:

Using germfree mouse as experimental material, it is arranged 1 group of blank, damages 1 group, 3 groups of control is (each right for above-described embodiment 1-3 Answer one group), every group 5；

(1) blank group smears back of mice only with the distilled water of 100 μ L, twice a day, continuous processing one week；

(2) using acetone-diethyl ether, to back of mice processing 30s, (acetone-diethyl ether repeats to contact and can draw damage group with skin Rise dry skin and it is dry and cracked), be spaced 30min after use water process 30s, twice a day, continuous processing one week；

(3) control group first uses acetone-diethyl ether to handle 30s to back of mice, uses water process 30s after being spaced 30min, later Respectively with the Abelmoschus esculentus fruit Essence 100ul in above-described embodiment, it is applied to each control group mice skin lesion sites, one day Twice, continuous processing one week.

By above-mentioned experiment, there is the scales of skin that peel off agglomerated in damage group back, and control group smears the position of Essence, do not agglomerate Phenomenon generates, and wherein the skin of control group mice is better than blank group, illustrates that Abelmoschus esculentus fruit Essence has significant protect Wet moisturizing effect.

Experimental verification two:

In order to further analyze and verify above three Abelmoschus esculentus fruit Essence in moisturizing, anti-inflammatory, anti-oxidant aspect Effect also has detected the opposite of LOR (loricrin) relevant to skin barrier and IVL (involucrin) in above-mentioned experiment Expression quantity, the antiphlogistic effects of the inflammatory factors such as TNF-α (tumor necrosis factor) and IL-6 (interleukin 6), as a result as shown in Figs 1-4 (loricrin and involucrin are the constitutive protein of cutin coating in skin barrier, for maintaining skin moisture-keeping and barrier function It can play an important role).

Experimental result shows that Abelmoschus esculentus fruit Essence can promote the expression of LOR and IVL, and then maintain skin barrier Function, promote skin moisturizing and moisturizing；From the point of view of anti-inflammatory experimental result, the TNF-α of three Abelmoschus esculentus fruit Essences and IL-6 relative expression quantity is below damage group, keeps the coffee obtained with expression similar in blank group, i.e. three embodiments Ambrette fruit Essence can have inhibitory effect to TNF-α and IL-6, illustrate that Abelmoschus esculentus fruit Essence prepared by this patent has There are good antiphlogistic effects.

Product beta test recruitment evaluation:

(1) trial product: Abelmoschus esculentus fruit Essence obtained by embodiment 1-3, distilled water, by embodiment two modes gained A liquid, B liquid；

(2) crowd: women is tried out, the age: 20-40 years old, totally 60 people, skin health, no skin allergy history；

(3) it is grouped: being by age divided into 6 groups, every group of 10 people；

(4) test method: being tested when compared with dry weather, and trier respectively uses above-mentioned trial product one sooner or later daily It is secondary, it is used continuously ten days, does not during which use other cosmetics, to trial product from replenishing water and preserving moisture, anti-inflammatory anti-oxidant two side after trying out Evaluation is made in face of the effect of trial product；

(5) use result: evaluation result is as follows:

Table 2 is the evaluation to trial product replenishing water and preserving moisture effect.

Table 2

Table 3 is the evaluation to the anti-inflammatory anti-oxidation efficacy of trial product.

Table 3

Evaluation result is tried out as table 2 and table 3 it is found that Abelmoschus esculentus fruit Essence obtained by embodiment 1-3 obtains mostly Number user approves there is apparent moisturizing moisturizing and anti-inflammatory anti-oxidation efficacy, and the effect of Abelmoschus esculentus fruit Essence will get well In single effect of A liquid and B liquid, wherein to the degree of recognition of Abelmoschus esculentus fruit Essence made from embodiment two be slightly better than other two Kind embodiment.

The preferred embodiment of the patent is described in detail above, but this patent is not limited to above-mentioned embodiment party Formula within the knowledge of one of ordinary skill in the art can also be under the premise of not departing from this patent objective It makes a variety of changes.

Claims (7)

1. a kind of Abelmoschus esculentus fruit Essence, characterized in that comprise the following raw materials by weight percent:

Abelmoschus esculentus fruit extracting solution 70%~85%

Glycerol 5%~15%

Propylene glycol 8%~20%

Natural antibacterial agent 0.01%~0.1%

Natural 0.05%~0.5%.

2. a kind of Abelmoschus esculentus fruit Essence according to claim 1, characterized in that the Abelmoschus esculentus fruit extracting solution is The water extract of Abelmoschus esculentus fruit.

3. a kind of Abelmoschus esculentus fruit Essence according to claim 2, characterized in that the Abelmoschus esculentus fruit extracting solution The preparation method comprises the following steps: weighing fresh coffee ambrette fruit, impurity is cleaned, is sliced 2~4mm sheet, puts into extraction element, is first added 1 ~3 times of distillation flooding 60min, Extracting temperature are 40~50 DEG C, and with filtered through gauze, filtrate is abandoned；2~4 are added in filter residue The distillation flooding of amount continues to extract 60min again, and Extracting temperature is 40~50 DEG C, with filtered through gauze, slightly extruding filter residue, makes to mention Liquid is taken sufficiently to flow out；Obtained extracting solution is centrifuged, revolving speed be 8000~10000r/min, centrifugation time be 5~ 10min, obtaining supernatant is Abelmoschus esculentus fruit extracting solution.

4. a kind of Abelmoschus esculentus fruit Essence according to claim 1, characterized in that the natural antibacterial agent is mentioned by Cortex Magnoliae Officinalis Take object, capillary extract, shrubby sophora extract, nutgall extractive, one of common cnidium fruit P.E or a variety of compositions.

5. a kind of Abelmoschus esculentus fruit Essence according to claim 1, characterized in that the natural is by natural Vitamin C, natural VE, Rosmarinus officinalis extract, one of tea polyphenols or a variety of compositions.

6. a kind of Abelmoschus esculentus fruit Essence according to claim 1, characterized in that by the raw material of following weight percent Composition:

Abelmoschus esculentus fruit extracting solution 74.78%

Glycerol 9.97%

Propylene glycol 14.95%

Natural antibacterial agent 0.05%

Natural 0.25%.

7. a kind of preparation method of the Abelmoschus esculentus fruit Essence as described in claim 1-6 is any, characterized in that including walking as follows It is rapid:

Step 1: weighing fresh coffee ambrette fruit, impurity is cleaned, is sliced 2~4mm sheet, puts into extraction element, is first added 1 ~3 times of distillation flooding 60min, Extracting temperature are 20~60 DEG C, and with filtered through gauze, filtrate is abandoned；2~4 are added in filter residue The distillation flooding of amount continues to extract 60min again, and Extracting temperature is 20~60 DEG C, with filtered through gauze, slightly extruding filter residue, makes to mention Liquid is taken sufficiently to flow out；

Step 2: obtained extracting solution is centrifuged, revolving speed is 8000~10000r/min, and centrifugation time is 5~10min, Supernatant is Abelmoschus esculentus fruit extracting solution；

Step 3: the preparation of A liquid: weighing the glycerol of formula ratio, Abelmoschus esculentus fruit extracting solution and glycerol be sufficiently mixed It is even to obtain A liquid；

Step 4: the preparation of B liquid: propylene glycol, the natural antibacterial agent, natural of formula ratio are weighed respectively, in temperature 50 Under the conditions of DEG C, three is stirred dissolution, obtains clear liquid, which is B liquid；

Step 5: A liquid and B liquid are stirred and evenly mixed by high-speed shearing machine, Abelmoschus esculentus fruit Essence is obtained.