CN109833378A - A kind of injection of wind-expelling pain-stopping and its preparation method and application - Google Patents
A kind of injection of wind-expelling pain-stopping and its preparation method and application Download PDFInfo
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- CN109833378A CN109833378A CN201910243510.4A CN201910243510A CN109833378A CN 109833378 A CN109833378 A CN 109833378A CN 201910243510 A CN201910243510 A CN 201910243510A CN 109833378 A CN109833378 A CN 109833378A
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Abstract
The present invention provides a kind of injection and its preparation method and application of wind-expelling pain-stopping, belong to technical field of extraction of Chinese traditional medicine, the injection of the wind-expelling pain-stopping includes Daphne giraldii Nitsche extract, the Daphne giraldii Nitsche extract is obtained by carrying out alcohol extracting after first distilling girald daphne bark again, and the Daphne giraldii Nitsche extract is made especially by following steps: the Daphne giraldii Nitsche extract being made especially by following steps: the girald daphne bark after drying being ground into so cotton-shaped that expect only；Obtained net material plus water for injection are repeatedly distilled again, distillate is collected and refrigerates；Then the material residue after distillation is subjected to alcohol and raises extracting solution refrigeration；Finally distillate and extracting solution are mixed and obtain Daphne giraldii Nitsche extract.The present invention can significantly improve the recovery rate of 50-80%, to save crude drug, auxiliary material, reduce production cost, and injection of the invention has preferable anti-inflammatory and antalgic, blood circulation invigorating efficacies by the way that girald daphne bark first to be distilled to the technique of alcohol extracting again.
Description
Technical field
The present invention relates to technical field of extraction of Chinese traditional medicine, and in particular to a kind of injection of wind-expelling pain-stopping, in addition the present invention is gone back
It is related to the preparation method and application of the injection of above-mentioned wind-expelling pain-stopping.
Background technique
Girald daphne bark is Thymelaeceae Thymelaeaceae daphne plant D.giraldii Nitsche Doalphne giraidii Nitsche
Root skin and stem skin, congener Tang Gute winter daphne Daphne.tanautica Maxim, retuseleaf daphne bark D.retusaHemsl,
Korean daphne root and stem D.aphne raveane Naka can be used as medicine.Girald daphne bark has the effect of dispelling wind and eliminating dampness, analgesic scattered silt, therefore wide
It is general to be used to treat pain, traumatic injury, rheumatic arthritis and bronchitis, divide studies have shown that being extracted mainly in girald daphne bark
Has the effects that analgesia, anti-inflammatory, antibacterial, antitumor from obtained dephnetin ingredient.
Extraction for effective component in girald daphne bark, as disclosed in granted patent CN1785314B with dehydrated alcohol or
The method that the alcohol of 30-90% is impregnated to obtain filtrate is mentioned in granted patent CN 102008599B and measures 60- using 5-20 times
90% alcohol reflux extracts the method for obtaining extracting solution three times, is equally mentioned with alcohol reflux in publication CN108721439A
The method processing girald daphne bark taken, i.e., the extraction of current girald daphne bark's effective component carry out refluxing extraction simply by ethyl alcohol, in this way
Cause extraction efficiency lower.
Summary of the invention
Problem based on the above background, the present invention provide a kind of injection of wind-expelling pain-stopping comprising Daphne giraldii Nitsche extract,
Middle Daphne giraldii Nitsche extract is obtained by carrying out alcohol extracting after first distilling girald daphne bark again, and recovery rate is high；Another mesh of the invention
The injection for being to provide above-mentioned wind-expelling pain-stopping preparation method and application.
In order to achieve the above objectives, technical scheme is as follows:
A kind of injection of wind-expelling pain-stopping, the injection of the wind-expelling pain-stopping include Daphne giraldii Nitsche extract, the girald daphne bark
Extract is obtained by carrying out alcohol extracting after first distilling girald daphne bark again.
Wherein, the content of daphnetin is 0.2-0.55mg/ml in the Daphne giraldii Nitsche extract.
Wherein, the Daphne giraldii Nitsche extract is made especially by following steps: the girald daphne bark after drying being ground into cotton-shaped
It obtains and expects only；Obtained net material plus water for injection are repeatedly distilled again, distillate is collected and refrigerates；Then by the material after distillation
Slag carries out alcohol and raises extracting solution refrigeration；Finally distillate and extracting solution are mixed and obtain Daphne giraldii Nitsche extract.
Preferably, the proportion of the net material and water for injection is 1g:8-15ml, the proportion of the distillate and water for injection
For 0.2-0.7:1.
Preferably, the alcohol extracting is that material residue is obtained extracting solution, the material residue in alcohol solution for soaking 12-48h at normal temperature
Proportion with alcoholic solution is 1g:5-15ml.
It is highly preferred that the alcoholic solution is the ethanol solution that concentration is 30-85%.
Preferably, the extracting solution that the alcohol extracting obtains is subjected to thickening filtration and obtains filtered fluid, by filtered fluid tune pH to 3.0-
It filters to boil after secondary filter liquid, secondary filter liquid tune pH to 1.0-2.0 after 4.0 refrigeration 24-72h and flow back at 96-100 DEG C
45-60min obtains phegma, and by phegma tune pH to 6.8-7.2, pH to 7-8 is adjusted in filtering after refrigeration.
Wherein, the injection of the wind-expelling pain-stopping is suitable for intravenous drip, acupuncture point, intramuscular injection.
The present invention also provides the preparation methods of the injection of above-mentioned wind-expelling pain-stopping, comprising the following steps: after drying
Girald daphne bark is ground into so cotton-shaped that expect only；Obtained net material plus water for injection are repeatedly distilled again, distillate is collected and refrigerates；
Then the material residue after distillation is subjected to alcohol and raises extracting solution refrigeration；Finally mixing distillate and extracting solution is to obtain girald daphne bark to mention
Object is taken, obtained Daphne giraldii Nitsche extract is diluted to the injection that wind-expelling pain-stopping can be obtained.
The present invention also provides the injections of above-mentioned wind-expelling pain-stopping in treatment rheumatic Bi syndrome, traumatic injury, apoplexy and cancer etc.
Application in pain.
Compared with prior art, the invention has the following advantages:
1, the technique of the invention by the way that girald daphne bark first to be distilled to alcohol extracting again, can significantly improve the recovery rate of 50-80%, from
And crude drug, auxiliary material have been saved, production cost is reduced, and injection of the invention has preferable anti-inflammatory and antalgic, promoting blood circulationization
The stasis of blood, wind-dispelling are reduced swelling effect；2, the injection of wind-expelling pain-stopping of the invention can not add auxiliary material, thus can be possible to avoid auxiliary material
The curative effect and caused side effect influenced.
Specific embodiment
The invention belongs to technical field of extraction of Chinese traditional medicine, primarily to a kind of injection of wind-expelling pain-stopping is provided, it is described to dispel
The injection of wind analgesic includes Daphne giraldii Nitsche extract, and the Daphne giraldii Nitsche extract is by carrying out alcohol after first distilling girald daphne bark again
It raises.Technique by first distilling alcohol extracting again can significantly improve the extraction efficiency of Daphne giraldii Nitsche extract, and saving is produced into
This.
The present invention will be further described in detail below with reference to specific embodiments, Recipe used in embodiment
Only some optimization formulas and technique in the present invention, but protection scope of the present invention is not restricted to this.Also need to illustrate
It is that following solution for adjusting pH are the sodium hydroxide solution of the hydrochloric acid solution that mass fraction is 10%-20% and 40%.
Embodiment 1
It after examining qualified girald daphne bark's clean dry, is ground into cotton-shaped obtain and expects only, it is spare to enter net material library；It obtains again
Net material 10g adds water for injection 150ml successively to be distilled twice, collects distillate 105ml and refrigerates；Then it is added into material residue
The alcohol solution dipping that 150ml concentration is 75% impregnates for 24 hours, impregnates diafiltration thickening filtration recycling alcohol and obtains filtered fluid；Incited somebody to action
Filtrate tune pH to 4.0 filters to boil after secondary filter liquid, secondary filter liquid tune pH to 2.0 and at 96-100 DEG C after refrigerating 72h
Reflux 60min obtains phegma, and phegma tune pH to 7.2 is refrigerated.Finally distillate and extracting solution are mixed and obtain girald daphne bark
Extract, the content of daphnetin is 0.32mg/ml in obtained Daphne giraldii Nitsche extract.
The measuring method of daphnetin content is: the 1. preparation of reference substance solution: precision weighs daphnetin control
Appropriate product add methanol that the solution in every lml containing 0.2mg is made；2. accurate respectively draw reference substance solution and the present embodiment solution
Each 20 μ l, inject liquid chromatograph, be filler with octadecylsilane chemically bonded silica, with -0.5% acetum of methanol (30:
It 70) is mobile phase, Detection wavelength 327nm, number of theoretical plate is calculated by daphnetin peak should be not less than 2000.
Embodiment 2
It after examining qualified girald daphne bark's clean dry, is ground into cotton-shaped obtain and expects only, it is spare to enter net material library；It obtains again
Net material 10g adds water for injection 80ml successively to be distilled twice, collects distillate 16ml and refrigerates；Then it is added into material residue
The alcohol solution dipping that 100ml concentration is 85% impregnates 12h, impregnates diafiltration thickening filtration recycling alcohol and obtains filtered fluid；Incited somebody to action
The refrigeration of filtrate tune pH to 3.0 filters to boil after secondary filter liquid, secondary filter liquid tune pH to 1.0 and at 96-100 DEG C for 24 hours afterwards
Reflux 45min obtains phegma, and phegma tune pH to 6.8 is refrigerated, and finally adjusts pH to 7；Finally distillate and extracting solution are mixed
It closes and obtains Daphne giraldii Nitsche extract, the content of daphnetin is 0.2mg/ml in obtained Daphne giraldii Nitsche extract.
Embodiment 3
It after examining qualified girald daphne bark's clean dry, is ground into cotton-shaped obtain and expects only, it is spare to enter net material library；It obtains again
Net material 120g adds water for injection 1100ml successively to be distilled twice, collects distillate 600ml and refrigerates；Then add into material residue
Enter the alcohol solution dipping that 600ml concentration is 30%, impregnate 18 hours, impregnate diafiltration thickening filtration recycling alcohol and is filtered
Liquid；Will filtered fluid tune pH to 3.5 refrigerate 48h after filter to boil after secondary filter liquid, secondary filter liquid tune pH to 1.5 and
96-100 DEG C of reflux 50min obtains phegma, and phegma tune pH to 7.0 is refrigerated, girald daphne bark's first in obtained Daphne giraldii Nitsche extract
The content of element is 0.4mg/ml.
Embodiment 4
It after examining qualified girald daphne bark's clean dry, is ground into cotton-shaped obtain and expects only, it is spare to enter net material library；It obtains again
Net material 120g adds water for injection 1100ml successively to be distilled twice, collects distillate 600ml and refrigerates；Then add into material residue
Enter the alcohol solution dipping that 600ml concentration is 75%, impregnate 48 hours, impregnate diafiltration thickening filtration recycling alcohol and is filtered
Liquid；Will filtered fluid tune pH to 3.5 refrigerate 48h after filter to boil after secondary filter liquid, secondary filter liquid tune pH to 1.5 and
96-100 DEG C of reflux 50min obtains phegma, and phegma tune pH to 7.0 is refrigerated, girald daphne bark's first in obtained Daphne giraldii Nitsche extract
The content of element is 0.55mg/ml.
Comparative example
It after examining qualified girald daphne bark's clean dry, is ground into cotton-shaped obtain and expects only, it is spare to enter net material library；It obtains again
The alcohol solution dipping that 600ml concentration is 75% is added in net material 120g, impregnates 48 hours, impregnates diafiltration thickening filtration recycling alcohol simultaneously
Obtain filtered fluid；Will filtered fluid tune pH to 3.5 refrigerate 48h after filter to boil after secondary filter liquid, secondary filter liquid tune pH to 1.5
It boils and obtains phegma in 96-100 DEG C of reflux 50min, phegma tune pH to 7.0 is refrigerated, ancestral in obtained Daphne giraldii Nitsche extract
The content of teacher's fiber crops A prime is 0.15mg/ml.
The toxicity of the obtained injection of the present invention is tested first, mainly observation intravenous administration and abdominal cavity note
To acute toxic reaction caused by mouse and death condition after penetrating, and observe its local irritation and the influence to red blood cell.
Specific test process is as follows:
1, test material
A, animal: Kunming mouse, half male and half female, weight 18-22g；Rabbit, New Zealand's large ear rabbit, weight 2.5-
3.0kg is provided by Shanxi provincial institute for drug control animal housing.
B, drug: injection prepared by embodiment 3, experiment are configured to sterile saline before use with for concentrated wiring liquid
Required concentration.
2, method and result
A, LD of the intravenous injection administration to mouse50
Mouse is taken to be divided into 5 groups at random by weight, by intravenous injection administration, dosage is by 1:0.8 agent away from sterile physiological salt
Water proportional diluted, fasting 12h before mouse is administered, but can't help water, and administration capacity is 0.4ml/20g, it is then observed continuously seven days,
Day by day the mouse toxicity reaction recorded and death toll, calculate the LD of mouse with Bliss method with NDST program50And 95% fiducial limit,
The results are shown in Table 1.
The LD of the intravenous injection administration of table 150Experimental result
Significant sex index: G=0.1989 X50=2.7507 Sx=0.0237 G are smaller, have been omitted from
Heterogeneity checks: Chi2=1.55 Chi2.05=7.82 Sb=2.2271 are without heterogeneity
Regression equation are as follows: Y (Probit) %=-21.0197+9.788*1og (D) r=0.9621
LD50=563.2129 ± 85.6717 (484.0198-655.3632) mg/kg
Felller heterogeneity correction: Sx=0.0190 Sb=1.6006
LD50=563.2129 ± 69.2204 (498.2223-638.6811) mg/kg
It is available by table 1, LD of the injection intravenous injection administration of the present invention to mouse50For 563.2 ± 69.2mg/kg,
After being injected intravenously injection of the present invention, mouse is had a convulsion at once, writhing phenomenon, then quadruped locomotion obstacle, shallow breathing, most
Stop death to breathing afterwards, is dissected, visually observed, main organs pathological anatomy inspection is showed no obvious abnormalities change.
B, the acute toxicity test of intraperitoneal injection
Take this heavy 20 ± 2g mouse 20, half male and half female.Disposable abdomen when mouse fasting 12h, but can't help water, Yu Shangwu 8
Chamber drug administration by injection, administration capacity is 0.5ml/10g, is then observed continuously 7 days, records mouse toxicity reaction day by day.As a result it gives
After medicine in 7 days, no abnormality seen toxic reaction, hair color, activity, diet, the excrement of mouse is normal.To eye, mucous membrane and maincenter mind
Through system also without influence.After 7 days, to weigh, male mouse increases to 27.02 ± 2.24g by preceding 19.65 ± 1.23g (X ± SD) is administered,
Growth rate is 37.51%；Female mice is increased to by preceding 19.52 ± 1.25g is administered as 25.87 ± 1.91g, growth rate 32.5%, corpse
Inspection visually observes the main organs heart, liver, board, the phenomenon without exception such as lung, kidney, thymus gland, adrenal gland.
C, exempt from the local stimulation test of quadriceps muscle of thigh to family
Rabbit 2 are taken, respectively injects injection 1ml of the present invention in its two leg quadriceps muscle of thigh of left and right respectively, 48 is small after injection
When put to death animal, quadriceps muscle of thigh is taken out in dissection, and longitudinally slit, observation injection site benefit swashs reaction, and records corresponding thorn by table 2
Swash intensity.The result shows that the congested range mean value of two rabbit is 0.5cm, stimulus intensity is 1 grade of (±) reaction, illustrates that this product is pierced
Sharp property is smaller.
The degree table of grading of 2 rabbit quadriceps muscle of thigh stimulate the reaction of table
D, red blood cell shape is rung and is tested
6, test tube are taken, 2% red blood cell suspension and physiological saline are sequentially added by 3 proportional quantity of table, after mixing, in 37 DEG C
Insulating box places half an hour, is then respectively adding different amounts of medical fluid (the 6th pipe is control tube).After shaking up, it is placed in 37 DEG C of constant temperature
In case.Start every 15min observation once, it is primary every observation in 1 hour after 1h, it observes 4 hours.Such as the transparent red of solution,
Indicate haemolysis；As having brownish red or rufous wadding dress precipitating in solution, erythroagglutination is indicated.As a result 1-5 pipe is equal
Haemolysis and coacervation are not had.
Influence of the table 3 to red blood cell
Next injection the present embodiment 4 being prepared carries out following pharmacodynamics test:
One, anti-inflammatory effect
1, test material
Injection prepared by embodiment 4, hydrocortisone injection, the second pharmaceutical factory, Datong District 931119 batches；Big white mouse
Wistar kind, small white mouse Kunming kind are provided by Mountain Western Medicine S University's Experimental Animal Center.
2, experimental method and result
A, to the influence of rat albumen arthritis.
Rat 40 are taken, test group high and low dose, hydrocortisone, 4 groups of control group, single administration are randomly divided into.Wherein
Test group high dose is that rat intramuscular injection is given with the dosage of 1ml/kg, and test group low dosage is to give rat flesh with the dosage of 0.5ml/kg
Note.It is administered after 15min and every mouse right hind foot plantar is injected with 10% egg white physiological salt liquid 0.05ml, measurement note egg white front and back is not
With or so time ankle-joint perimeter, difference is swelling, the results are shown in Table 4.0.5-2 hours after egg white causes inflammation, test group is high
Low dosage plays the role of obviously inhibiting scorching swollen, and effect time of occurrence can be similar with hydrogen.
The influence that table 4 swells to rat egg white vola pedis
Note: compared with the control group: P<0.01 * P>0.05, * * P<0.05, * * *
B, the effect swollen to rat carrageenan vola pedis
Big white mouse 40 are taken, test group high and low dose, hydrocortisone (intramuscular injection 50mg/kg) and control group 4 are randomly divided into
Group, wherein test group high dose is that rat intramuscular injection is given with the dosage of 1ml/kg, and test group low dosage is with the dosage of 0.5ml/kg
Give rat intramuscular injection.15min after administration, with 1% carrageenan physiological salt liquid 0.05mI note, foot plantar is subcutaneous after every mouse is right, this
Different time measures left and right hind leg ankle-joint perimeter afterwards, and difference is swelling, the results are shown in Table 5.Test group high dose Yu Zhiyan
There is the inhibiting effect of highly significant to 5h afterwards, the action effect of low dosage is approximate with hydrocortisone.
The influence that table 5 swells to rat carrageenan vola pedis
Note: compared with the control group: P<0.01 * P>0.05, * * P<0.05, * * *
C, to the effect of rat formaldehyde swollen joint
Big white mouse 40 are taken, is randomly divided into 4 groups of test high and low dose, aspirin and control group etc., wherein test group is high
Dosage is that rat intramuscular injection is given with the dosage of 1ml/kg, and test group low dosage is to give rat intramuscular injection with the dosage of 0.5ml/kg.Intramuscular injection
After 15min is administered, foot plantar is subcutaneously injected into 2.5% formalin 0.05ml and causes scorching, different time measurement thereafter after every mouse is right
Left and right hind leg ankle-joint perimeter, difference are right side swelling.Continue to be administered within the 2nd day after to formaldehyde, and 0.5h after administration
Measurement left and right hind leg ankle-joint perimeter finds out from 6 result of table, compared with the control group, test group high dose in formaldehyde cause it is scorching after 2h extremely
7d has the scorching swollen effect of apparent mitigation, and effect is better than Ah woods, and the effect of low dosage is more obvious in 2-8h, thereafter
Drug effect has then weakened.
Influence of the table 6 to rat formaldehyde swollen joint
Note: compared with the control group: P<0.01 * P>0.05, * * P<0.05, * * *
Two, analgesic activity
1, hot plate method
Take female mice, with hot plate method select the normal threshold of pain 30 seconds or less person 40, be randomly divided into high and low dose of test group
Amount, strong pain, 4 groups of control group.Intramuscular administration 1 time, 0.5 and 1h, surveys its pain threshold with hot plate method after administration.From 7 result of table
As it can be seen that test group high and low dose can make the threshold of pain extend 50% or more, compared with the control group, difference has the meaning of highly significant
Justice.
Influence of the table 7 to hot plate method in mice pain valve
2, mouse writhing method
Mouse 40,18-22g are taken, half male and half female is randomly divided into test group high and low dose, strong pain, 4 groups of control group.
15min after intramuscular administration injects 0.6%HAC 10ml/kg, observes the writhing number in 15min immediately.It the results are shown in Table 8.As a result
Show that test group high and low dose group can be reduced writhing number caused by acetic acid.
8 Dichlorodiphenyl Acetate of table causes the influence of pain mouse effect
Note: compared with the control group: P<0.01 * P>0.05, * * P<0.05, * * *
Finally, clinical treatment is used for injection of the invention, through Yongji the People's Hospital, hospital, Yuncheng Prefecture, Shanxi Province
The clinical observation of the units such as the People's Hospital, Fu Feng hospital, Shanxi Medical College, 421 analgesias are efficient up to 89.5%, wherein wound
Property pain 89.7%, rheumatalgia 88.9%, cancer pain 83.5%, Algopsychalia 93%, abdominal pain 91.9%.Concrete outcome such as table 9-13
It is shown.
The analgesic activity of 9 421 pain cases of table is analyzed
Efficacy analysis of the table 10 to abdominal pain
Table 11 is to neuralgic efficacy analysis
Efficacy analysis of the table 12 to rheumatic pain
Efficacy analysis of the table 13 to Cancerous pain
It should be noted that the administrated method of above-mentioned abdominal pain, neuralgia, cancer pain is a 2-4ml, two times a day.
Above-mentioned abdominal pain, neuralgia, cancer pain efficacy assessment standard are: effective, pain wholly or substantially disappears in 5-15min after medication
It loses；Effectively, pain relief or there is mitigation in 15-30min after medication；In vain, after medication after 35min without alleviate and medication before without change
Change.
The administrated method of rheumatic pain: a 2ml, 2 times a day, treatment time are 4 weeks；Rheumatic pain efficacy evaluation mark
Quasi-: effective: the cardinal symptoms such as pain, swelling are eliminated, and function of joint is restored substantially, can participate in normal work and labour, physics and chemistry inspection
It is normal to look into index；Effective: the cardinal symptoms such as pain, swelling substantially eliminate, and major joint function is restored substantially or had obvious
Progress, physiology, which cannot take care of oneself, switchs to can take care of oneself or lose labour and ability to work is effectively restored, main physical and chemical inspection indicators
It is effectively improved；It is invalid: and make comparisons before treatment, without progress.
Typical case:
Cao XX, female, 35 years old, group, the city Yongji County Xia Gao member.Sciatica 6 years, usually difficulty in walking,
It cannot do manual work, through traditional Chinese and western medicine multi-treatment, without being clearly better, use 4 injection of the present embodiment, the injection of HUANTIAO acupoint deep later
2-4ml, 3min pain relief can walk freely, 15-30min analgesic, duration 8h.Once a day, continuous 12 caves
It injects and is cured in position.Follow-up 2 years without recurrence.
Old XX, male, 40 years old, xx army.Right side paroxysmal nervous headache three days feels fidgety, through injecting after attaching the names of pre-determined candidates
Pain relief after this injection 4ml, 5min, 9min pain disappear, work as usual.
Wei XX, female, 58 years old, Yongji County Zhang Ying group member was advanced gastric cancer, Huan Zheshang through Yongji County hospital diagnosis
Abdomen is in great pain, feels restless, and through with calm ineffective with general upper pain medicine, with this injection 2ml, abdominal pain disappears after 5min
It loses, maintains 8h.
Go into business XX, male, and 28 years old, Xi Cun group member.Because working accidentally, iron milling shovel is hurt on right leg at one third,
Wound is about two centimeters, and 1 point 5 centimeter deep, the conscious lower limb pain of patient cannot acutely walk, and carries out Local treatment, injection is originally
After injection 4ml, 3min mitigated, and 5min pain is alleviated substantially, energy Go to ground activity, later daily injection 4ml, in the 8th day redness
Pain completely disappears, wound healing, participates in productive labor within the 10th day.
Bandit XX, male, 48 years old, Xi Cun group member.Suffer from gastric ulcer 3 years, when it is good when violate, when criminal abdominal pain unbearably, through with
The antispasmodics such as antondin and atropine, belladonna are ineffective, after use this injection 2ml instead, intramuscular injection, pain disappears after 3min
It loses.
Claims (10)
1. a kind of injection of wind-expelling pain-stopping, which is characterized in that the injection of the wind-expelling pain-stopping includes Daphne giraldii Nitsche extract, institute
Stating Daphne giraldii Nitsche extract is obtained by carrying out alcohol extracting after first distilling girald daphne bark again.
2. the injection of wind-expelling pain-stopping according to claim 1, which is characterized in that girald daphne bark in the Daphne giraldii Nitsche extract
The content of A prime is 0.2-0.55mg/ml.
3. the injection of wind-expelling pain-stopping according to claim 1, which is characterized in that the Daphne giraldii Nitsche extract especially by
Following steps are made: the girald daphne bark after drying being ground into so cotton-shaped that expect only；Obtained net material plus water for injection are carried out again more
Secondary distillation is collected distillate and is refrigerated；Then the material residue after distillation is subjected to alcohol and raises extracting solution refrigeration；Finally by distillate and
Extracting solution mixing obtains Daphne giraldii Nitsche extract.
4. the injection of wind-expelling pain-stopping according to claim 3, which is characterized in that the proportion of the net material and water for injection
For 1g:8-15ml, the proportion of the distillate and water for injection is 0.2-0.7:1.
5. the injection of wind-expelling pain-stopping according to claim 3, which is characterized in that the alcohol extracting is at normal temperature by material residue
Extracting solution is obtained in alcohol solution for soaking 12-48h, and the proportion of the material residue and alcoholic solution is 1g:5-15ml.
6. the injection of wind-expelling pain-stopping according to claim 5, which is characterized in that the alcoholic solution is that concentration is 30-
85% ethanol solution.
7. the injection of wind-expelling pain-stopping according to claim 3, which is characterized in that the extracting solution for obtaining the alcohol extracting into
Row thickening filtration obtains filtered fluid, filters to obtain secondary filter liquid, secondary filter after filtered fluid tune pH to 3.0-4.0 is refrigerated 24-72h
It is boiled after liquid tune pH to 1.0-2.0 and obtains phegma in 96-100 DEG C of reflux 45-60min, by phegma tune pH to 6.8-7.2,
PH to 7-8 is adjusted in filtering after refrigeration.
8. the injection of -7 any wind-expelling pain-stoppings according to claim 1, which is characterized in that the injection of the wind-expelling pain-stopping
Agent is suitable for intravenous drip, acupuncture point, intramuscular injection.
9. a kind of preparation method of the injection of wind-expelling pain-stopping as claimed in claim 1, which is characterized in that including with
Lower step: the girald daphne bark after drying is ground into so cotton-shaped that expect only；Obtained net material plus water for injection are repeatedly distilled again,
It collects distillate and refrigerates；Then the material residue after distillation is subjected to alcohol and raises extracting solution refrigeration；Finally by distillate and extracting solution
Mixing obtains Daphne giraldii Nitsche extract, and obtained Daphne giraldii Nitsche extract is diluted to the injection that wind-expelling pain-stopping can be obtained.
10. a kind of injection of wind-expelling pain-stopping as claimed in claim 1 treatment rheumatic Bi syndrome, traumatic injury, in
Application in the pain such as wind and cancer.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311236963.7A CN117717584A (en) | 2019-03-28 | 2019-03-28 | Application of girald daphne bark extract in preparing medicines for treating swelling and pain |
| CN201910243510.4A CN109833378A (en) | 2019-03-28 | 2019-03-28 | A kind of injection of wind-expelling pain-stopping and its preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910243510.4A CN109833378A (en) | 2019-03-28 | 2019-03-28 | A kind of injection of wind-expelling pain-stopping and its preparation method and application |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311236963.7A Division CN117717584A (en) | 2019-03-28 | 2019-03-28 | Application of girald daphne bark extract in preparing medicines for treating swelling and pain |
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| Publication Number | Publication Date |
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| CN109833378A true CN109833378A (en) | 2019-06-04 |
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|---|---|---|---|
| CN202311236963.7A Pending CN117717584A (en) | 2019-03-28 | 2019-03-28 | Application of girald daphne bark extract in preparing medicines for treating swelling and pain |
| CN201910243510.4A Pending CN109833378A (en) | 2019-03-28 | 2019-03-28 | A kind of injection of wind-expelling pain-stopping and its preparation method and application |
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| CN202311236963.7A Pending CN117717584A (en) | 2019-03-28 | 2019-03-28 | Application of girald daphne bark extract in preparing medicines for treating swelling and pain |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1857702A (en) * | 2006-03-20 | 2006-11-08 | 甘肃省药物研究所 | Detoxicated girald daphne and its externally applied transdermal absorption preparation and preparing process |
| CN1883618A (en) * | 2005-06-21 | 2006-12-27 | 天津药物研究院 | Effective parts of cirald daphne bark, preparation method and application thereof |
| CN101474315A (en) * | 2008-12-26 | 2009-07-08 | 南京中医药大学 | Effective component group of stem-bark or root-bark of Daphne giraldii as well as preparation method and use thereof |
-
2019
- 2019-03-28 CN CN202311236963.7A patent/CN117717584A/en active Pending
- 2019-03-28 CN CN201910243510.4A patent/CN109833378A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1883618A (en) * | 2005-06-21 | 2006-12-27 | 天津药物研究院 | Effective parts of cirald daphne bark, preparation method and application thereof |
| CN1857702A (en) * | 2006-03-20 | 2006-11-08 | 甘肃省药物研究所 | Detoxicated girald daphne and its externally applied transdermal absorption preparation and preparing process |
| CN101474315A (en) * | 2008-12-26 | 2009-07-08 | 南京中医药大学 | Effective component group of stem-bark or root-bark of Daphne giraldii as well as preparation method and use thereof |
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| Title |
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| 中华人民共和国卫生部药典委员会: "《中华人民共和国卫生部药品标准 中药成方制剂 第18册 保护品种分册二》", 31 December 1998 * |
| 唐得时: "《中药化学》", 30 November 1986, 人民卫生出版社 * |
| 巢建国: "《中药资源学》", 31 August 2014, 中国医药出版社 * |
| 董培智: "祖师麻注射液的抗炎作用研究", 《山西医科大学学报》 * |
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