CN109832126A - A kind of paddy rice dual-purpose line with genic sterile propagation method - Google Patents
A kind of paddy rice dual-purpose line with genic sterile propagation method Download PDFInfo
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Abstract
The invention belongs to the planting technology fields of rice, disclose a kind of paddy rice dual-purpose line with genic sterile propagation method；It include: to establish two-line sterile line fertility sensitive safe period model；Establish two-line sterile line safe spiking and flowering period model；It determines best meteorological site: screening the meteorological data for the multiple meteorological sites in the whole nation being collected into, select the meteorological site for meeting two security models；It determines the best sowing time: according to the meteorological site filtered out and most safe heading period, in conjunction with the duration from seeding to heading of two-line sterile line, calculating the best sowing time of the meteorological site under different origin temps；Dual purpose genic male sterile line breeding: the best place filtered out carries out the sowing plantation of paddy rice dual-purpose line with genic sterile as paddy rice dual-purpose line with genic sterile breeding base, according to the sowing time filtered out.Present invention understands that the problem of certainly Hainan in Winter breeding risk is big, yield is not high, seed quality is poor and cold water string irrigating reproductive output not high unstable and inefficiencies.
Description
Technical field
The invention belongs to the planting technology field of rice more particularly to a kind of paddy rice dual-purpose line with genic sterile propagation methods.
Background technique
Currently, the prior art commonly used in the trade is such thatThe main method of paddy rice dual-purpose line with genic sterile breeding at present has
Two kinds: first method is cold water string irrigating method, when sterile line growth and development enters or so 3 phase of fertility-sensitive period~4 phases
It waits, cold water irrigation is carried out to breeding field by way of groundwater abstraction or reservoir water flooding, irrigation time is according to sterile line
Developmental state it is different, substantially irrigate 14 days or so, sterile line growth and development terminates cold when entering 7 phase of fertility-sensitive period
It fills；Second method is the method taken in Hainan Island winter natural propagation, and annual winter is found in Hainan and suitably clicks through
Row plantation, the origin temp of each sterile line is different, and the requirement to natural weather is also different, the weather in annual Hainan rise and fall compared with
Greatly.But there are water resource limitations for the method for cold water string irrigating, often exist with Production of Large Fields and use water contradiction, the requirement to water temperature
Very high with irrigation conditions requirement, cost is big, and reproductive output is low, and the excessively high then reproductive failure of temperature, temperature is too low, meets fertility
Obstacle, yield are very low；Hainan in Winter breeding is because of Hainan weather ups and downs, and the origin temp of different sterile lines is different, to certainly
The requirement of right weather is different, and the success rate of breeding is lower, depends entirely on weather conditions and sowing time and the place of current year, no
Controllable factor is too many, and thus there are not high unstable, the seed matter of yield for the paddy rice dual-purpose line with genic sterile propagation method of current main-stream
Measure the problem poor, benefit is low.
In conclusion problem of the existing technology is:
(1) method of cold water string irrigating is limited by water resource, very high to water temperature and irrigation conditions requirement, and cost is big, numerous
Grow low output, the excessively high reproductive failure of temperature, the too low chance growing barrier of temperature, low output.
(2) Hainan in Winter is bred, weather ups and downs, and the origin temp of different sterile lines is different, is wanted to natural weather
Ask different, the success rate of breeding is lower.
So the paddy rice dual-purpose line with genic sterile propagation method of current main-stream is there are the not high shakiness of yield, seed quality is poor, imitates
The low problem of benefit.
Solve the difficulty and meaning of above-mentioned technical problem:The breeding safety issue of paddy rice dual-purpose line with genic sterile is limitation two
The significant obstacle of linear hybrid rice development is the hang-up encountered during double-linear hybrid rice is promoted, and cold water string irrigating and Hainan winter are numerous
Ideally average product 150kg/ mus or so, there are huge risk of failure when more, solve this significant problem
For pushing the development of double-linear hybrid rice to have positive important meaning.
Summary of the invention
In view of the problems of the existing technology, the present invention provides a kind of paddy rice dual-purpose line with genic sterile propagation methods.
The present invention, by the meteorological data that screening is in all parts of the country, is sought by establishing paddy rice dual-purpose line with genic sterile fertility model
It finds and meets fertility model needs, the solution space of Weather-stable carries out the breeding of paddy rice dual-purpose line with genic sterile, on the ground filtered out
Point can carry out safe high yield breeding, push the development and growth of double-linear hybrid rice.
The invention is realized in this way a kind of paddy rice dual-purpose line with genic sterile propagation method, the paddy rice dual-purpose line with genic sterile
Propagation method includes: to establish sterile line fertility sensitive safe period model；Establish sterile line safe spiking and flowering period model；Determine gas
As website: screening the meteorological data for 740 meteorological sites in the whole nation being collected into, select the weather station for meeting two security models
Point；Determine the sowing time: according to the duration from seeding to heading of sterile line, when calculating the best sowing of the meteorological site under different origin temps
Between；Dual purpose genic male sterile line breeding: it is planted according to optimum paddy rice dual-purpose line with genic sterile breeding base and sowing time is filtered out
Dual purpose genic male sterile line is planted according to conventional rice implantation methods.
Further, during fertility sensitive in 16 days without daily mean temperature >=critical temperature of sterility for three days on end or≤16 DEG C, it is defeated
The time started gathers out.
Further, whether the anywhere for filtering out weather information has the safety for allowing two-line sterile line to carry out Fertility al-teration
Timing node retrodicts out the sowing time according to sterile line breeding time.
Further, between heading flowering period in 8 days without daily mean temperature >=30 DEG C for three days on end or≤21 DEG C, export meteorological point
Set.
Further, in conjunction with fertility sensitive safe period model, screening meets the place of fertility sensitive safe period requirement, assessment ground
Whether point can allow sterile line to be capable of safe heading flowering.
Further, the meteorological data for 740 meteorological sites in the whole nation being collected by model discrimination, searches out and meets two
The place of security model.
Further, according to the duration from seeding to heading of sterile line, the sowing time of meteorological site under different origin temps is calculated.
Further, dual purpose genic male sterile line is carried out according to filtering out paddy rice dual-purpose line with genic sterile breeding base and sowing time
Plantation.
Further, the paddy rice dual-purpose line with genic sterile propagation method specifically includes:
Step 1, data preparation, screening meet the meteorological site data of rice growth, incongruent weather station points
According to whole deletions；Condition 1: being based on rice growth feature, and place of 2000 meters greater than height above sea level is to be not suitable for carrying out rice not
Educating is breeding, and the breeding base of screening only selects meteorological point of the height above sea level lower than 2000 meters of suitable paddy growths；Condition 2:
Data needs 10 years or more of all meteorological points, otherwise data do not have representativeness；Meet the meteorological site of 2 conditions above
It sorts in alphabetical order, the data to all meteorological sites is needed to screen；
Step 2, sterile line fertility sensitive safe period model discrimination, two-line sterile line enter the fertility-sensitive period fourth phase and open
Begin to need in 16 days without daily mean temperature >=critical temperature of sterility for three days on end or≤16 DEG C；Detailed process is as follows:
(1) settling time set SS, preliminary examination value D0For the website the earliest date in database, the mean daily temperature on the same day is taken
DT；
(2) judge mean daily temperature DT and origin temp QT, different sterile line origin temps is different, generally 22 DEG C~
Between 24 DEG C, select to determine by user before calculating: if DT > QT, dangerous day, initial value 0) add up D=D+1, if dangerous
Day >=3, then mean that the temperature for three days on end since some date does not meet sterile line propagation requirement, which does not enter
Safety time set SS gathers initial value D0=D0+ 1, mean daily temperature DT, 1 He of repetitive process are taken since next date
2；If DT≤QT, safe day S (initial value 0)=S+1, dangerous day D clear 0；
(3) judge whether safe day S reaches 16 days, if S < 16, the mean daily temperature for removing one day is repeated as DT
Process 1,2,3；Each date can judge whether continuous 16 days thereafter meet the requirements, if the date in S=16, D value is just
In formula entry time set SS, shows all to meet sterile line propagation condition in the date backward 16 days, be the safe date；
Meet, be inserted into time set, marks the fertility sensitive safe period safety of the time；
(4) after having recycled all data, each place can set up a time set, and the inside includes multiple dates,
Date is exactly the safe date for meeting two-line sterile line breeding, some date, fertility-sensitive period was more if the duplicate time limit is more
Safety is the important evidence for judging probability below；
Step 3, sterile line safe spiking and flowering period model discrimination；Sterile line started after heading in 8 days without day for three days on end
Temperature on average >=30 DEG C or≤21 DEG C guarantee optimum weather condition during two-line sterile line heading poplar bloassom, self-fruitful rate
It is obviously improved；
(1) date value is taken in safety time set SS before in order, fertility has screened safely what completion obtained
Date set, preliminary examination value D0Mean daily temperature DT for the earliest date in data set, after taking 20 days dates；
(2) judge whether mean daily temperature DT is greater than 30 DEG C: if 30 DEG C of DT >, add up dangerous day D=D+1, if danger
Dangerous day >=3 then mean that the temperature for three days on end since some date does not meet sterile line heading poplar bloassom and requires, data
Concentrate the safe spiking and flowering period on the date labeled as dangerous, set initial value D0Per day temperature is taken since next date
Spend DT, repetitive process 1 and 2；If DT≤21 DEG C, add up low temperature danger day LD=LD+1, the pumping on the date if LD=3
Fringe flowering safe period is labeled as dangerous；If DT≤30 DEG C, safe day S (initial value 0)=S+1, High Temperature day HD clear 0,
Low temperature danger day LD clear 0；
(3) judge whether safe day S reaches 8 days；If S < 8, the mean daily temperature for removing one day was repeated as DT
Journey 1,2,3；If in the date formal entry time set SS in S=8, D value, shown all full in the date backward 8 days
Sufficient sterile line heading poplar bloassom condition, is the safe date, and the heading flowering period safety on the date is marked in time set SS.
To sum up, after having recycled all data, each place can set up a time set, and the inside includes multiple days
Phase, date are exactly the safe date for meeting two-line sterile line heading poplar bloassom, some date ears if the duplicate time limit is more
Phase is safer；
Step 4, determines the safe probability in place: passing through 740, the whole nation gas that is collected into of Cycle Screening Step 2: three
As the meteorological data of website, the place for meeting two security models and safe probability are selected, the place of data set does not indicate to be somebody's turn to do
Place is not suitable for carrying out two-line sterile line breeding；
Calculate fertility safe probability: the number that some date occurs in data acquisition system carries out probability calculation；Calculate heading poplar
Flower safe probability: the number that some date occurs in data acquisition system carries out probability calculation；
Calculate whole safe probability: whole safe probability=fertility safe probability * heading poplar bloassom safe probability；
Later by simple ranking function, the highest when and where of safe probability can be filtered out；
Step 5 determines the best sowing time: according to the duration from seeding to heading of some sterile line, calculating should under different origin temps
The best sowing time of meteorological site；Best=calculated best the fertility sensitive time started+20 days-sowing time broadcasts the beginning and goes through
- 80 days on the 26th phase=January=best sowing time；
Step 6, dual purpose genic male sterile line breeding: according to filter out optimum paddy rice dual-purpose line with genic sterile breeding base and
Sowing time plants dual purpose genic male sterile line, plants according to conventional rice implantation methods, carries out the breeding of safe high yield.
In conclusion advantages of the present invention and good effect are as follows:The place that the present invention screens carries out paddy rice dual-purpose Genetic Sterility
The general planting of system can carry out the breeding of safe high yield, do not need the water source of cold water string irrigating and avoid Hainan winter numerous risk.
Solution Hainan in Winter breeding risk is big, yield is not high, seed quality is poor and the not high unstable and benefit of cold water string irrigating reproductive output
Low problem, is worthy of popularization.
Detailed description of the invention
Fig. 1 is the flow chart schematic diagram of paddy rice dual-purpose line with genic sterile propagation method provided in an embodiment of the present invention；
Fig. 2 is that paddy rice dual-purpose line with genic sterile propagation method provided in an embodiment of the present invention establishes sterile line fertility sensitive safety
The schematic diagram of phase model flow；
Fig. 3 is that paddy rice dual-purpose line with genic sterile propagation method provided in an embodiment of the present invention establishes sterile line heading flowering safety
The schematic diagram of phase model flow.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
Paddy rice dual-purpose line with genic sterile propagation method specific steps provided in an embodiment of the present invention include:
S101: sterile line fertility sensitive safe period model is established；
S102: sterile line safe spiking and flowering period model is established；
S103: it determines meteorological site: screening the meteorological data for 740 meteorological sites in the whole nation being collected into, select and meet two
The place of a security model；
S104: it determines the best sowing time: according to the duration from seeding to heading of sterile line, calculating the weather station under different origin temps
The best sowing time of point；
S105: it dual purpose genic male sterile line breeding: optimum paddy rice dual-purpose line with genic sterile breeding base and is broadcast according to filtering out
The kind time plants dual purpose genic male sterile line, plants according to conventional rice implantation methods, carries out the breeding of safe high yield.
Paddy rice dual-purpose line with genic sterile propagation method provided in an embodiment of the present invention specifically includes the following steps:
Step 1, data preparation.Screening meets the meteorological site data of rice growth, incongruent weather station points
According to whole deletions.Condition 1: being based on rice growth feature, and place of 2000 meters greater than height above sea level is to be not suitable for carrying out rice not
Educating is breeding, therefore the breeding base screened only selects meteorological point of the height above sea level lower than 2000 meters of suitable paddy growths.Item
Part 2: data needs 10 years or more of all meteorological points, otherwise data do not have representativeness；Meet the meteorology of 2 conditions above
Website sorts in alphabetical order, and the data to all meteorological sites is needed to screen；
Step 2, (two-line sterile line enters the fertility-sensitive period fourth phase and opens sterile line fertility sensitive safe period model discrimination
Begin to need without daily mean temperature >=critical temperature of sterility for three days on end or≤16 DEG C in 16 days, this model is extremely important, only full
The weather of this condition of foot can guarantee that two-line sterile line generates pollen, can be selfed explanation, can guarantee that breeding is
Safety；
Detailed process is as follows (referring to Fig. 2):
1, settling time set SS, preliminary examination value D0For the website the earliest date in database, the mean daily temperature on the same day is taken
DT；
2, judge mean daily temperature DT and origin temp QT (different sterile line origin temps is different, generally 22 DEG C~
Between 24 DEG C, select to determine by user before calculating): if DT > QT, dangerous day (initial value 0) is added up D=D+1, if endangered
Dangerous day >=3 then mean that the temperature for three days on end since some date does not meet sterile line propagation requirement, the date not into
Enter safety time set SS, gathers initial value D0=D0+ 1, mean daily temperature DT, repetitive process 1 are taken since next date
With 2.If DT≤QT, safe day S (initial value 0)=S+1, dangerous day D clear 0；
3, judge whether safe day S reaches 16 days, if S < 16, the mean daily temperature for removing one day is repeated as DT
Process 1,2,3；Each date can judge whether continuous 16 days thereafter meet the requirements, if the date in S=16, D value is just
In formula entry time set SS, shows all to meet sterile line propagation condition in the date backward 16 days, be the safe date；
Meet, be inserted into time set, marks the fertility sensitive safe period safety of the time.
4, after having recycled all data, each place can set up a time set, and the inside includes multiple dates, this
A little dates are exactly the safe date for meeting two-line sterile line breeding, some date fertility-sensitive period if the duplicate time limit is more
Safer, this is the important evidence for judging probability below.
Step 3, sterile line safe spiking and flowering period model discrimination；Sterile line started after heading in 8 days without day for three days on end
Temperature on average >=30 DEG C or≤21 DEG C guarantee optimum weather condition during two-line sterile line heading poplar bloassom, self-fruitful rate
It is obviously improved, is of great significance to the yield for improving breeding；
Detailed process is as follows (referring to Fig. 3):
1, taking date value in safety time set SS before in order, (fertility has screened safely the day for completing to obtain
Phase set), preliminary examination value D0For the earliest date in data set, take after 20 days dates that (the fertility sensitive time started is flat to beginning ear period
Be 20 days) mean daily temperature DT；
2, judge whether mean daily temperature DT is greater than 30 DEG C (per day temperature >=30 DEG C are very unfavorable to heading poplar bloassom): if
30 DEG C of DT >, dangerous day (initial value 0) is added up D=D+1, if dangerous day >=3, mean the company since some date
Continuous 3 days temperature do not meet sterile line heading poplar bloassom and require, and the safe spiking and flowering period on the date in data set is labeled as uneasiness
Entirely, gather initial value D0Mean daily temperature DT, repetitive process 1 and 2 are taken since next date.If DT≤21 DEG C (are lower than
21 degrees Celsius of heading poplar bloassoms encounter low temperature risk), add up low temperature danger day LD=LD+1, the pumping on the date if LD=3
Fringe flowering safe period is labeled as dangerous；If DT≤30 DEG C, safe day S (initial value 0)=S+1, High Temperature day HD clear 0,
Low temperature danger day LD clear 0；
3, judge whether safe day S reaches 8 days.If S < 8, the mean daily temperature for removing one day was repeated as DT
Journey 1,2,3；If in the date formal entry time set SS in S=8, D value, shown all full in the date backward 8 days
Sufficient sterile line heading poplar bloassom condition, is the safe date, and the heading flowering period safety on the date is marked in time set SS.
4, after to sum up, having recycled all data, each place can set up a time set, and the inside includes multiple days
Phase, these dates are exactly the safe date for meeting two-line sterile line heading poplar bloassom, some date is if the duplicate time limit is more
Heading stage is safer.This is the important evidence for judging probability below.Finally formed time set is such that
| Website | Date | Screen origin temp | Fertility-sensitive period safe period | Ear poplar bloassom safe period |
| Sanya | 1986-1-6 | 22.5℃ | Safety | Safety |
| Sanya | 1986-1-7 | 22.5℃ | Safety | Safety |
| Sanya | 1986-1-8 | 22.5℃ | Safety | It is dangerous |
| Sanya | 1986-1-9 | 22.5℃ | It is dangerous | Safety |
| Sanya | 1986-1-10 | 22.5℃ | It is dangerous | It is dangerous |
| ... | ... | ... | ... | ... |
Step 4, determines the safe probability in place: passing through 740, the whole nation gas that is collected into of Cycle Screening Step 2: three
As the meteorological data of website, the place for meeting two security models and safe probability are selected, the place of data set does not indicate to be somebody's turn to do
Place is not suitable for carrying out two-line sterile line breeding；
Calculate fertility safe probability: the number progress probability calculation that some date occurs in data acquisition system, such as Hainan
January 6 occurred 20 times (fertility-sensitive period label safety) in 30 years data in data set, then fertility safe probability is 20/
30=66.6%.
Calculate heading poplar bloassom safe probability: the number that some date occurs in data acquisition system carries out probability calculation, such as sea
In the time set SS in south, there are 27 (heading poplar bloassom phases in 30 years data in the heading poplar bloassom safe period label on January 6
Label safety), then fertility safe probability is 27/30=90%.
Whole safe probability: whole safe probability=fertility safe probability * heading poplar bloassom safe probability is calculated, it is above
The data instance in face Sanya January 6, the safe probability=66.6%*90%=59.9% in Sanya January 6
Ultimately form safe probability statistical form
Pass through simple ranking function later, it will be able to filter out the highest when and where of safe probability.
Step 5 determines the best sowing time: according to the duration from seeding to heading of some sterile line, calculating should under different origin temps
The best sowing time of meteorological site；Best sowing time=calculated best fertility sensitive time started (such as Sanya 1
Months 6 days)+20 days (generalized time)-duration from seeding to heading -80 days on the 26th=November 7 (such as 80 days)=January are (when best sowing
Between)；
Step 6, dual purpose genic male sterile line breeding: according to filter out optimum paddy rice dual-purpose line with genic sterile breeding base and
Sowing time plants dual purpose genic male sterile line, plants according to conventional rice implantation methods, carries out the breeding of safe high yield.
It is as shown in Figure 3: during the fertility sensitive of S101 in 16 days without daily mean temperature >=critical temperature of sterility for three days on end or
≤ 16 DEG C, output time started set；
Whether the anywhere that weather information can be filtered out has the peace that two-line sterile line can be allowed to carry out Fertility al-teration
Full-time intermediate node illustrates that the place is not suitable for doing the breeding base of two-line sterile line if the timing node not being suitble to, if
Suitable then model can provide the suitable fertility sensitive time, then retrodict out the suitable sowing time according to sterile line breeding time；
In S102 between heading flowering period in 8 days without daily mean temperature >=30 DEG C for three days on end or≤21 DEG C, export meteorological point set
It closes；
In conjunction with fertility sensitive safe period model, screening meets the place of fertility sensitive safe period requirement, assesses these places
Sterile line whether can be allowed to be capable of the heading flowering of safety；
By the meteorological data for 740 meteorological sites in the whole nation that model discrimination is collected into, searches out and meet two safe moulds
The meteorological site of type；
According to the duration from seeding to heading of sterile line, the best sowing time of the meteorological site under different origin temps is calculated；
Dual purpose genic male sterile line is carried out according to optimum paddy rice dual-purpose line with genic sterile breeding base and sowing time is filtered out
Plantation, according to conventional rice implantation methods plant, do not need other cultivation management measure, carry out the breeding of safe high yield.
Below with reference to embodiment, the present invention is described further:
The paddy rice dual-purpose line with genic sterile propagation method that embodiment 1 provides utilizes 50 years meteorologies of national 740 meteorological sites
Data, appliance computer processing technique develop paddy rice dual-purpose line with genic sterile propagation method, and utilize the screening system optimum
Paddy rice dual-purpose line with genic sterile breeding base.The system establishes sterile line fertility sensitive safe period model and safe spiking and flowering period
Model, and to meet sterile line fertility sensitive safe period and safe spiking and flowering period light and temperature condition simultaneously as foundation, it uses
Java language is worked out.
Using this system to 740 meteorological calculating for putting nearly 20,000,000 original meteorological datas, for critical temperature of sterility
For 22.0 DEG C, 22.5 DEG C, 23.0 DEG C, 23.5 DEG C and 24.0 DEG C of dual purpose genic male sterile line, filter out respectively numerous compared with the Sanya, Hainan winter
Breeding base preferably is respectively 24,29,20,21 and 22 (in fact, each type of paddy rice dual-purpose line with genic sterile only needs
Screen 1~2 optimal breeding base).The paddy rice dual-purpose line with genic sterile for being 22 DEG C for critical temperature of sterility, Yunnan are protected
Mountain is its best breeding base, and the probability that breeding fertility sensitive safe period and safe spiking and flowering period are all satisfied is 83.34%,
Much higher than same type paddy rice dual-purpose line with genic sterile Sanya, Hainan winter numerous safety 35.0% (table 1).For sterile starting point temperature
The paddy rice dual-purpose line with genic sterile that degree is 22.5 DEG C, 23.0 DEG C, 23.5 DEG C and 24.0 DEG C, Lincang is optimal breeding base, numerous
The probability that kind fertility sensitive safe period and safe spiking and flowering period are all satisfied is respectively 93.48%, 100%, 100% and 100%,
It is numerous obviously higher than the Sanya winter (table 2) that its fertility-sensitive period safety and heading flowering period safety meet probability simultaneously.It is selecting
On the basis of selecting optimum breeding base, reasonably and accurately arranging fertility-sensitive period is the key that reproductive success, this system according to
Maximum probability superposition principle extrapolates the most suitable fertility sensitive of different base difference critical temperature of sterility paddy rice dual-purpose line with genic sterile
Phase from date (Tables 1 and 2).User can arrange optimum sowing time according to the duration from seeding to heading of sterile line, utmostly
It is upper to guarantee its breeding safety.
The breeding of paddy rice dual-purpose line with genic sterile has been carried out using the Baoshan, Yunnan base that this system screens.2010,
It is 3.6hm that area, which is scheduled, in the Baoshan, Yunnan Shidian County township Shui Chang2Experimental field, it is numerous to carry out 22 DEG C of critical temperature of sterility of C815S
It grows and plants experimentally.April 10 sowing, May 25 transplanting, August Qi Sui on the 18th, September 28 maturation.On September 27th, 2010, Hunan Province
Relevant expert survey producing and check and accept to breeding scene in organizing nation, Science and Technology Department, calculates by 13.5% water content, and reproductive output reaches
8437.5kg/hm2, China's paddy rice dual-purpose line with genic sterile reproductive output tidemark is created, is also confirmed that using the department of computer science
System selection Dual purpose male-sterile line breeding base is effective.
Therefore, the breeding of paddy rice dual-purpose line with genic sterile is carried out using the breeding base that this system screens, it is expected to solve Hainan
Winter breeding risk is big, yield is not high, seed quality is poor and cold water string irrigating reproductive output is not high unstable and inefficiencies asks
Topic, is worthy of popularization
The paddy rice dual-purpose line with genic sterile propagation method that embodiment 2 provides carries out the breeding of safe high yield:
Paddy rice dual-purpose line with genic sterile is a kind of dual-purpose type rice, and different fertility shapes is shown under different light and temperature conditions
State realizes different germplasm purposes.If in the rice growth later period, into fertility-sensitive period (3~6 phase of ear differentiation)
When, lower than critical temperature of sterility, (different sterile lines shows difference to continuous several days environment mean temperatures, generally at 22 DEG C
Between~24 DEG C), sterile line will sterile changeover, become normally generate pollen, complete the plant of self-pollination, thus complete
At the process of self-reproduction.If environment temperature is higher than critical temperature of sterility, sterile line just without pollen, can not self-pollination,
It needs to receive pollen from the external world, to achieve the purpose that preparing hybrid combines.So the difficult point in dual purpose genic male sterile line breeding exists
In suitable environment temperature, temperature is excessively high, can not generate pollen, can not breed；The too low then physiology infertility of temperature, can not also produce
Raw pollen, can not breed.
The paddy rice dual-purpose line with genic sterile breeding base information of 22 DEG C of 1 critical temperature of sterility of table
The most suitable breeding base of paddy rice dual-purpose line with genic sterile and Sanya, Hainan meteorological condition of 23.5 DEG C of 2 critical temperature of sterility of table
Meet probability to compare
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within mind and principle.
Claims (9)
1. a kind of paddy rice dual-purpose line with genic sterile propagation method, which is characterized in that the paddy rice dual-purpose line with genic sterile propagation method packet
It includes: establishing sterile line fertility sensitive safe period model；Establish sterile line safe spiking and flowering period model；Determine meteorological site: sieve
The meteorological data for selecting 740 meteorological sites in the whole nation being collected into, selects the meteorological site for meeting two security models；Determine sowing
Time: according to the duration from seeding to heading of sterile line, the best sowing time of the meteorological site under different origin temps is calculated；Dual-purpose core is not
Educate is breeding: planting dual-purpose Genetic Sterility according to optimum paddy rice dual-purpose line with genic sterile breeding base and sowing time is filtered out
System plants according to conventional rice implantation methods.
2. paddy rice dual-purpose line with genic sterile propagation method as described in claim 1, which is characterized in that during fertility sensitive in 16 days
Without daily mean temperature >=critical temperature of sterility for three days on end or≤16 DEG C, output time started set.
3. paddy rice dual-purpose line with genic sterile propagation method as described in claim 1, which is characterized in that filter out weather information
Whether anywhere has the safety time node for allowing two-line sterile line to carry out Fertility al-teration, is retrodicted out and is broadcast according to sterile line breeding time
The kind time.
4. paddy rice dual-purpose line with genic sterile propagation method as described in claim 1, which is characterized in that between heading flowering period in 8 days
Without daily mean temperature >=30 DEG C for three days on end or≤21 DEG C, meteorological point set is exported.
5. paddy rice dual-purpose line with genic sterile propagation method as described in claim 1, which is characterized in that in conjunction with fertility sensitive safe period
Model, screening meet the place of fertility sensitive safe period requirement, and whether assessment place can allow sterile line to be capable of safe heading
Flowering.
6. paddy rice dual-purpose line with genic sterile propagation method as described in claim 1, which is characterized in that be collected by model discrimination
740 meteorological sites in the whole nation meteorological data, search out the place for meeting two security models.
7. paddy rice dual-purpose line with genic sterile propagation method as described in claim 1, which is characterized in that gone through according to the beginning of broadcasting of sterile line
Phase calculates the sowing time of meteorological site under different origin temps.
8. paddy rice dual-purpose line with genic sterile propagation method as described in claim 1, which is characterized in that according to filtering out paddy rice dual-purpose
Line with genic sterile breeding base and sowing time carry out the plantation of dual purpose genic male sterile line.
9. paddy rice dual-purpose line with genic sterile propagation method as described in claim 1, which is characterized in that the paddy rice dual-purpose Genetic Sterility
It is that propagation method specifically includes:
Step 1, data preparation, screening meet the meteorological site data of rice growth, and incongruent meteorological site data are complete
It deletes in portion；Condition 1: being based on rice growth feature, and place of 2000 meters greater than height above sea level is to be not suitable for carrying out rice sterile line
Breeding, the breeding base of screening only selects meteorological point of the height above sea level lower than 2000 meters of suitable paddy growths；Condition 2: all
Data needs 10 years or more of meteorology point, otherwise data do not have representativeness；Meet above 2 conditions meteorological site according to
Lexicographic order sequence, needs the data to all meteorological sites to screen；
Step 2, sterile line fertility sensitive safe period model discrimination, two-line sterile line enter the fertility-sensitive period fourth phase start need
It takes in 16 days without daily mean temperature >=critical temperature of sterility for three days on end or≤16 DEG C；Detailed process is as follows:
(1) settling time set SS, preliminary examination value D0For the website the earliest date in database, the mean daily temperature DT on the same day is taken；
(2) judge mean daily temperature DT and origin temp QT, different sterile line origin temps is different, generally at 22 DEG C~24 DEG C
Between, select to determine by user before calculating: if DT > QT, dangerous day, initial value 0) add up D=D+1, if dangerous day >=
3, then mean that the temperature for three days on end since some date does not meet sterile line propagation requirement, which does not enter safety
Time set SS gathers initial value D0=D0+ 1, mean daily temperature DT, repetitive process 1 and 2 are taken since next date；If
DT≤QT, safe day S (initial value 0)=S+1, dangerous day D clear 0；
(3) judge whether safe day S reaches 16 days, if S < 16, remove one day mean daily temperature as DT, repetitive process
1,2,3；Each date can judge whether continuous 16 days thereafter meet the requirements, if the date in S=16, D value formally into
In angle of incidence set SS, shows all to meet sterile line propagation condition in the date backward 16 days, be the safe date；Meet
It is then inserted into time set, marks the fertility sensitive safe period safety of the time；
(4) after having recycled all data, each place can set up a time set, and the inside includes multiple dates, date
Exactly meet the safe date of two-line sterile line breeding, some date fertility-sensitive period if the duplicate time limit is more is more pacified
It entirely, is the important evidence for judging probability below；
Step 3, sterile line safe spiking and flowering period model discrimination；Sterile line starts after heading in 8 days without per day for three days on end
Temperature >=30 DEG C or≤21 DEG C, guarantee optimum weather condition during two-line sterile line heading poplar bloassom, and self-fruitful rate is significant
It is promoted；
(1) date value is taken in safety time set SS before in order, fertility has screened safely the date for completing to obtain
Set, preliminary examination value D0Mean daily temperature DT for the earliest date in data set, after taking 20 days dates；
(2) judge whether mean daily temperature DT is greater than 30 DEG C: if 30 DEG C of DT >, add up dangerous day D=D+1, if dangerous day
>=3, then mean that the temperature for three days on end since some date does not meet sterile line heading poplar bloassom and requires, in data set
The safe spiking and flowering period on the date is labeled as dangerous, set initial value D0Mean daily temperature is taken since next date
DT, repetitive process 1 and 2；If DT≤21 DEG C, add up low temperature danger day LD=LD+1, the heading on the date if LD=3
Flowering safe period is labeled as dangerous；If DT≤30 DEG C, safe day S (initial value 0)=S+1 is High Temperature day HD clear 0, low
Warm danger day LD clear 0；
(3) judge whether safe day S reaches 8 days；If S < 8, one day mean daily temperature is removed as DT, repetitive process 1,
2,3；If in the date formal entry time set SS in S=8, D value, showing all to meet not in the date backward 8 days
Educating is heading poplar bloassom condition, is the safe date, and the heading flowering period safety on the date is marked in time set SS；
To sum up, after having recycled all data, each place can set up a time set, and the inside includes multiple dates, day
Phase is exactly the safe date for meeting two-line sterile line heading poplar bloassom, and heading stage if the duplicate time limit is more on some date more pacifies
Entirely；
Step 4, determines the safe probability in place: passing through 740, the whole nation weather station that is collected into of Cycle Screening Step 2: three
The meteorological data of point, selects the place for meeting two security models and safe probability, the place of data set does not indicate the place
Be not suitable for carrying out two-line sterile line breeding；
Calculate fertility safe probability: the number that some date occurs in data acquisition system carries out probability calculation；Calculate heading poplar bloassom peace
Full probability: the number that some date occurs in data acquisition system carries out probability calculation；
Calculate whole safe probability: whole safe probability=fertility safe probability * heading poplar bloassom safe probability；
Later by simple ranking function, the highest when and where of safe probability can be filtered out；
Step 5 determines the best sowing time: according to the duration from seeding to heading of some sterile line, calculating the meteorology under different origin temps
The best sowing time of website；Best sowing time=calculated best+20 days fertility sensitive time starteds-duration from seeding to heading=
- 80 days on the 26th January=best sowing time；
Step 6, dual purpose genic male sterile line breeding: according to filtering out optimum paddy rice dual-purpose line with genic sterile breeding base and sowing
Time plants dual purpose genic male sterile line, plants according to conventional rice implantation methods, carries out the breeding of safe high yield.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112021107A (en) * | 2020-09-08 | 2020-12-04 | 安徽省农业科学院水稻研究所 | Production method of core seeds of two-line photo-thermo-sensitive male sterile line of rice |
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