CN109832043A - Device and method for subterranean pest-insect bioassay - Google Patents
Device and method for subterranean pest-insect bioassay Download PDFInfo
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- CN109832043A CN109832043A CN201711225696.8A CN201711225696A CN109832043A CN 109832043 A CN109832043 A CN 109832043A CN 201711225696 A CN201711225696 A CN 201711225696A CN 109832043 A CN109832043 A CN 109832043A
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Abstract
The present invention relates to Laboratory Observation equipment technical field more particularly to a kind of device and methods for subterranean pest-insect bioassay.The device includes bottom plate, two baffles and two groups of side plates being oppositely arranged, two baffles are respectively arranged at the both ends of bottom plate, two groups of side plates are detachably arranged in the two sides of bottom plate and each baffle respectively, so as to form the space for being used for planting plants between bottom plate, baffle and two groups of side plates, and convenient for carrying out the test operation of lossless root system of plant at any time；Every group of side plate includes barn door and observation board, and each barn door is parallel respectively to be set to outside observation board, so as to it is lossless, intuitively observation of plant root growth situation and carry out subterranean pest-insect bioassay test.The apparatus structure is simple, ingenious, can easily carry out the plantation to measuring plants, it is not damaged remove, the operation such as transplanting, moreover it is possible to the growth of observation of plant root system and be compromised situation during raw test at any time, while facilitating test worm inoculation and test result inspection.
Description
Technical field
The present invention relates to Laboratory Observation equipment technical field more particularly to a kind of dresses for subterranean pest-insect bioassay
It sets and method.
Background technique
Subterranean pest-insect refers to the entire history of life or the life of the history of life in some stage in the soil, endangers foot end
(such as root system, root tuber, subterranean stem, Subterranean fruits), a kind of insect for newly sowing sub or close native table stem.Subterranean pest-insect type is more,
Distribution is wide, feeding habits are miscellaneous, can feeding cause harm the multiple kinds of crops such as corn and soybean, peanut.Main subterranean pest-insect monoid have grub,
Wireworm, cutworm, mole cricket etc. are distributed in national most of provinces and regions.Cause harm since it is hidden in the soil, to prevention and treatment
Bring great difficulty.Under current no-tillage, straw-returning tillage system reform being widely popularized, subterranean pest-insect has increasingly
Serious trend.The prevention and treatment of subterranean pest-insect at present brings serious threat still based on chemical pesticide, to soil and environment.
Screening has the crop varieties of natural resistance to subterranean pest-insect, or to underground after genetic modification (transgenic technology)
The resistant crop lines of pest, are to successfully manage one of green prevention and control method of subterranean pest-insect.Resistance crop Screening of Strains Against is first
Indoor bioassay is first carried out, process includes: planting plants, assessment vegetation growth state, inoculation test worm, generates to test worm
Harm carry out qualitative and quantitative observation, the subterranean pest-insect resistance for treating measuring plants is classified.For Transgenic Plant Lines,
It also needs to screen positive transformants before being inoculated with test worm to be retained or transplanted, removes negative strain.But since subterranean pest-insect is usual
It hides in the soil, plant root of causing harm, traditional pot experiment is difficult to the upgrowth situation of intuitive observation of plant root system and by ground
The degree that lower pest is caused harm, it is also difficult to plant be carried out in the case where not damaging root system of plant the operation such as to transplant, become ground
The bottleneck of lower pest indoor bioassay.
Summary of the invention
(1) technical problems to be solved
The technical problem to be solved in the present invention is to provide a kind of device and methods for subterranean pest-insect bioassay, can
Lossless, intuitively observation of plant root growth situation and progress subterranean pest-insect measurement work, can carry out lossless plant removal at any time
It is operated with transplanting, facilitates and carry out test worm inoculation and test result inspection.
(2) technical solution
In order to solve the above-mentioned technical problems, the present invention provides a kind of devices for subterranean pest-insect bioassay, including
Bottom plate, two baffles and two groups of side plates being oppositely arranged, two baffles are respectively arranged at the both ends of the bottom plate, two groups of institutes
State the two sides that side plate is detachably arranged in the bottom plate and each baffle respectively；Side plate described in every group includes barn door and sight
Drafting board, each barn door is parallel respectively to be set to outside observation board.
Further, it is removably correspondingly connected with by fixing component respectively between the two sides and bottom of side plate described in two groups
In the two sides of the bottom plate and each baffle, the bottom plate and each baffle are all set between two barn doors, and are overlapped on
In fixing component between two barn door bottoms.
Further, the fixing component includes screw rod and nut, the screw rod be set in two groups described between side plate, institute
One end outside weld for stating screw rod is connected to the nut, the other end one nut of outer pivot bush unit, two nuts point
It is not set to outside side plate described in each group.
Further, two baffles are respectively fixedly disposed on the both ends of the bottom plate, and two baffles
Two sides are flexibly connected with side plate described in two groups respectively.
Further, the bottom plate is equipped with one or more ventilative drain holes, more when the ventilative drain hole is multiple
A ventilative drain hole is evenly distributed along the length direction of the bottom plate.
Further, the both ends of the bottom plate are removably connected with baffle, the two sides difference of two baffles respectively
It is flexibly connected with side plate described in two groups, the bottom of the baffle is removably connect with the bottom plate.
Further, the bottom of each baffle is equipped with fixing bolt, and the fixing bolt passes through any described
It in gas drain hole, and is fixed by locking nut, each baffle is detachably arranged in any pre- of the bottom plate
If on position, position adjustment is carried out between each predeterminated position by any baffle, to change the bottom plate, each gear
Spatial volume between plate and two groups of side plates.
Further, the bottom plate passes through dog-ear connector and side plate detachable connection described in any group.
Further, the material of the barn door is that light shield layer is coated on non-transparent material or the barn door；It is described
Observation board is made of clear material.
The present invention also provides a kind of method for carrying out subterranean pest-insect bioassay using device as described above, features
It is, includes the following steps:
S1, immersion vegetable seeds to be measured are spare to after sprouting；
S2, assembling are used for the device of subterranean pest-insect bioassay, and described device includes that bottom plate, two baffles and two groups are opposite
The side plate of setting, two baffles are respectively arranged at the both ends of the bottom plate, the dismountable setting respectively of side plate described in two groups
In the two sides of the bottom plate and each baffle；Side plate described in every group includes barn door and observation board, each barn door point
Not parallel is set to outside observation board；
S3, subterranean pest-insect biological test matrix is packed into described device, it will be in the seed implanted device after budding
Matrix in；
S4, each nut for being located at the same side in described device is unclamped, dismantles the side visor and retains observation board, to see
Examine plant root growth situation；
S5, when plant strain growth to suitable period, the side plate of described device either side is removed, by the underground of suitable age
Pest is by certain worm strain than reinstalling side plate described in the side in access described device；
After the completion of S6, the step S5, time recording plant strain growth parameter barn door described in removable side and is protected therebetween
Stay the observation board, observation of plant root growth and by Harm；After a period of time, remove side plate described in side, remove to
Measuring plants check the root system victimization state to measuring plants, record related data；
S7, step S6 the data obtained is for statistical analysis, comparison subterranean pest-insect is to the Harms of different plants, i.e.,
It can.
(3) beneficial effect
Above-mentioned technical proposal of the invention has the advantages that the dress for subterranean pest-insect bioassay of the invention
Set and method in, device includes bottom plate, two baffles and two groups of side plates being oppositely arranged, and two baffles are respectively arranged at bottom plate
Both ends, two groups of side plates are detachably arranged in the two sides of bottom plate and each baffle respectively, so that bottom plate, two baffles and two groups of sides
The space for being used for planting plants is formed between plate, and convenient for carrying out the test operation of lossless root system of plant at any time；Wherein, every group of side
Plate includes barn door and observation board, and each barn door is parallel respectively to be set to outside observation board, barn door can according to observation and
Plantation needs to mount and dismount, so as to which lossless, intuitively observation of plant root growth situation and progress subterranean pest-insect are biological
Measurement test.The apparatus structure is simple, can easily carry out the operations such as the plantation to measuring plants, not damaged removal, transplanting, also
The growing state of observation of plant root system and it can be compromised situation during raw test at any time, easily carried out to measuring plants
The operations such as plantation, not damaged removal, transplanting, while facilitating test worm inoculation and test result inspection.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the device of the embodiment of the present invention one；
Fig. 2 is the top view of the device of the embodiment of the present invention one；
Fig. 3 is the partial structure diagram of the device of the embodiment of the present invention two；
Fig. 4 is the partial structure diagram of the device of the embodiment of the present invention three.
Wherein, 1, bottom plate；2, side plate；21, barn door；22, observation board；3, baffle；4, screw rod；5, nut；6, it breathes freely and drips
Water hole；7, fixing bolt；8, locking nut；9, dog-ear connector.
Specific embodiment
Embodiments of the present invention are described in further detail with reference to the accompanying drawings and examples.Following embodiment is used for
Illustrate the present invention, but cannot be used to limit the scope of the invention.
In the description of the present invention, unless otherwise indicated, the meaning of " plurality " is two or more.Term " on ",
The orientation or positional relationship of the instructions such as "lower", "left", "right", "inner", "outside", " front end ", " rear end ", " head ", " tail portion " is
It is based on the orientation or positional relationship shown in the drawings, is merely for convenience of description of the present invention and simplification of the description, rather than instruction or dark
Show that signified device or element must have a particular orientation, be constructed and operated in a specific orientation, therefore should not be understood as pair
Limitation of the invention.
Device and method provided in this embodiment for subterranean pest-insect bioassay can be tested in laboratory conditions
Subterranean pest-insect treats the Harm of measuring plants root system, to evaluate the resistance to measuring plants for subterranean pest-insect, and can be at any time
The operation such as lossless plant removal, transplanting and test worm inoculation, result inspection is carried out, Genes For Plant Tolerance subterranean pest-insect indoor biometrics are breached
Bottleneck greatly improves the working efficiency of Genes For Plant Tolerance subterranean pest-insect evaluation.
The structure of the present apparatus and the content of this method are described in detail below by way of four embodiments.
Embodiment one
As depicted in figs. 1 and 2, device described in the present embodiment one includes that 1, two baffle 3 of bottom plate and two groups are oppositely arranged
Side plate 2, two baffles 3 are respectively arranged at the both ends of bottom plate 1, and two groups of side plates 2 are detachably arranged in bottom plate 1 and baffle respectively
3 two sides so that between 1, two baffle 3 of bottom plate and two groups of side plates 2 formed be used for planting plants space, and convenient at any time into
The test operation of the lossless root system of plant of row；Wherein, every group of side plate 2 includes barn door 21 and observation board 22, each barn door 21
Parallel respectively to be set to outside observation board 22, barn door 21 can need to mount and dismount according to observation and plantation, so as to nothing
Damage, intuitively observation of plant root growth situation and progress subterranean pest-insect bioassay test.The apparatus structure is simple, Neng Goufang
Just the carry out plantation to measuring plants, not damaged removal, transplanting etc. operates, moreover it is possible to observation of plant during raw test at any time
The growing state of root system and be compromised situation, easily carry out the plantation to measuring plants, it is not damaged remove, the operation such as transplanting, together
When facilitate test worm inoculation with test result inspection.
In the present embodiment one, the two sides and bottom of two groups of side plates 2 pass through fixing component respectively and are removably correspondingly connected with
The two sides of bottom plate 1 and two baffles 3, bottom plate 1 and each baffle 3 are all set between two barn doors 21, and are overlapped on two
In fixing component between 21 bottom of barn door, preferably two baffles 3 are respectively fixedly disposed on the both ends of bottom plate 1, and two
The two sides of baffle 3 are flexibly connected with two groups of side plates 2 respectively, so that being formed between 1, two baffle 3 of bottom plate and two groups of side plates 2
The above-mentioned space for planting plants.
It is preferred that light shield layer is coated on barn door 21, to ensure the light-shading effect in planting space, barn door 21
Can be preferably using non-transparent material as its material, making it equally has above-mentioned light-shading effect；It is preferred that observation board 22 is by transparent
Material is made, after ensuring to remove barn door 21, can through observation board 22 it is clear and intuitive see plant root growth situation, together
When meet requirement to the observation of the qualitative, quantitative of subterranean pest-insect, the material of further preferred observation board 22 is glass, transparent plastic
Or one or more of transparent resin combination.
It is excellent in order to reliably realize the relationship that is detachably connected between two groups of side plates 2 and between any side plate 2 and bottom plate 1
Selecting fixing component includes screw rod 4 and nut 5, and screw rod 4 is set between two groups of side plates 2, and one end outside weld of screw rod 4 is connected to a spiral shell
Cap 5, the other end one nut 5 of outer pivot bush unit, two nuts 5 are respectively arranged at outside each group side plate 2.
Specifically, the quadrangle of side plate 2 and bottom center are respectively equipped with a mounting hole, two groups of side plates it is preferred that side plate 2 is rectangle
2 are oppositely arranged, wherein the barn door 21 of each group side plate 2 is arranged at the outside of observation board 22；Two are sequentially passed through by screw rod 4
In correspondence mounting hole on group 2 same position of side plate, so that two groups of side plates 2 are separately positioned on the both ends of screw rod 4, nut 5 is utilized
Two groups of side plates 2 are locked in the two sides of bottom plate 1 and baffle 3, two groups of side plates 2 are separated using bottom plate 1 and baffle 3, to ensure
Effective planting space is formed between two groups of side plates 2；When carrying out unclamping each when the observation of the root system of measuring plants or subterranean pest-insect measure
The nut 5 of a 4 the same side of screw rod, removes the barn door 21 of the side side plate 2, then again that each nut 5 one of the side is a pair of
The observation board 22 that side plate 2 should be tightened in is outer；When needing to carry out when the work such as the transplanting of measuring plants, removal, unclamp each
The nut 5 of 4 the same side of screw rod sequentially removes the barn door 21 and observation board 22 of the side side plate 2, wait the works such as transplant, remove
After the completion of work, by the observation board 22 of the side side plate 2 and barn door 21, sequentially correspondence is mounted on each screw rod 4 again, is then weighed
It newly tightens to correspond on each nut 5 of the side and be tightened in outside the observation board 22 of side plate 2.
It is further preferred that transparent and black two is respectively adopted in the observation board 22 and barn door 21 of the device of the present embodiment one
The acrylic board of kind of color is made, thickness is respectively 5mm and 2mm, and fixing component is determined using 304 stainless steel materials, diameter 5mm
For butterfly screws processed as screw rod 4, both ends add butterfly nut 5.
It should be noted that side plate 2 can also use other shapes, such as round, ellipse or polygon in addition to rectangle
Deng, guarantee that bottom plate 1 and baffle 3 between two groups of side plates 2 are reliably connected according to the setting position of the fixing component on side plate 2, with
And ensure to form effective planting space between two groups of side plates 2.
In order in planting plants, it is ensured that the draining of plant and breathe freely it is smooth, preferably on bottom plate 1 be equipped with one or more
The ventilative drain hole 6 of setting is penetrated through, further, the draining being correspondingly arranged with ventilative drain hole 6 can be set in the lower section of bottom plate 1
Slot can carry out further measuring work to collect the moisture overflowed.
Embodiment two
As shown in figure 3, the apparatus structure of the present embodiment two is basically the same as the first embodiment, something in common is repeated no more, no
Be with place: in the device of the present embodiment two, ventilative drain hole 6 on bottom plate 1 be it is multiple, multiple ventilative drain holes 6 are the bottom of along
The length direction of plate 1 is evenly distributed, and preferably multiple ventilative drain holes 6 are symmetrically evenly arranged into two along the length direction of bottom plate 1
Column, the both ends of bottom plate 1 are removably connected with baffle 3 respectively, and the two sides of two baffles 3 are flexibly connected with two groups of side plates 2 respectively,
The bottom of baffle 3 is removably connect with bottom plate 1.
In order to ensure the bottom of baffle 3 and the reliability for being detachably connected relationship of bottom plate 1, the bottom of preferably each baffle 3
Portion is equipped with fixing bolt 7, is passed through in any ventilative drain hole 6 being correspondingly arranged using fixing bolt 7, and pass through locking nut
8 are locked in the lower surface of bottom plate 1 with fixing bolt 7, to each baffle 3 is detachably arranged on bottom plate 1 corresponding
Any predeterminated position on, when needing to change the size of planting space, by any baffle 3 between each predeterminated position into
Line position sets adjustment, to change the spatial volume between bottom plate 1, each baffle 3 and two groups of side plates 2, can specifically unclamp locking screw
Mother 8, the fixing bolt 7 of 3 bottom of baffle is extracted out from ventilative drain hole 6, then the ventilative drain hole of corresponding insertion another location
6, to realize that the position of baffle 3 is adjusted.
Embodiment three
As shown in figure 4, the apparatus structure of the present embodiment three and embodiment two are essentially identical, something in common is repeated no more, no
Be with place: the bottom plate 1 of the present embodiment three is connect by dog-ear connector 9 with any group of side plate 2, to prevent dismounting side from hiding
It causes to be detached between the side plate 2 of bottom plate 1, baffle 3 and the other side when tabula rasa 21, effectively improves the connection reliability and stabilization of device
Property.
Example IV
The present embodiment four provides a kind of device application method, is based on described in any one of embodiment one to embodiment three
Device propose, which includes the following steps:
S1, first vegetable seeds to be measured impregnated 2-3 days in seedlings nursing plate, it is spare after sprouting；
S2, embodiment one is pressed to the described in any item apparatus structures of embodiment three, assemble for observing root system of plant and survey
Determine the device of subterranean pest-insect, device includes that 1, two baffle 3 of bottom plate and two groups of side plates 2 being oppositely arranged, two baffles 3 are set respectively
The both ends of bottom plate 1 are placed in, two groups of side plates 2 are detachably arranged in the two sides of bottom plate 1 and each baffle 3 respectively；Every group of side plate 2 is equal
Including barn door 21 and observation board 22, each barn door 21 is parallel respectively to be set to outside observation board 22.
S3, it then is packed into subterranean pest-insect biological test matrix (soil that such as humidity is about 20%) in the device, useful load
It is the 90% of device total measurement (volume), it will be in the soil in the seed implanted device that germinateed；
S4, each nut 5 for being located at the same side is unclamped in due course, dismantle the side black barn door 21 and retain transparent observation
Plate 22, observation of plant root growth situation；
S5, when plant grows to suitable period, the side plate 2 of device either side is removed, by the ground of suitable age
Lower pest (such as grub) than in access device, and the side side plate 2 is reinstalled by certain worm strain；
S6, step S5 connect worm after the completion of, time recording plant strain growth parameter, as plant plant height (or other indexs) number
According to removable side black barn door 21 and retaining transparent observation board 22 therebetween, observation of plant root growth and endangered feelings
Condition；After a period of time, side side plate 2 is removed, is removed to measuring plants, checks that the root system victimization state to measuring plants, record are related
Data；
S7, step S6 the data obtained is for statistical analysis, comparison subterranean pest-insect is to the Harms of different plants, i.e.,
It can.
In conclusion the present embodiment one, into example IV, this is used in the device and method of subterranean pest-insect bioassay,
Device includes that 1, two baffle 3 of bottom plate and two groups of side plates 2 being oppositely arranged, two baffles 3 are respectively arranged at the both ends of bottom plate 1,
Two groups of side plates 2 are detachably arranged in the two sides of bottom plate 1 and each baffle 3 respectively, so that 1, two baffle 3 of bottom plate and two groups of sides
The space for being used for planting plants is formed between plate 2, and convenient for carrying out the test operation of lossless root system of plant at any time；Wherein, every group
Side plate 2 includes barn door 21 and observation board 22, and each barn door 21 is parallel respectively to be set to outside observation board 22, barn door 21
It can need to mount and dismount according to observation and plantation, so as to lossless, intuitively observation of plant root growth situation and progress
Subterranean pest-insect bioassay test.The apparatus structure is simple, can easily carry out the plantation to measuring plants, it is not damaged remove,
The operation such as transplanting, moreover it is possible to observation of plant root growth situation and be compromised situation during raw test at any time, easily into
The operations such as plantation, not damaged removal, transplanting of the row to measuring plants, while facilitating test worm inoculation and test result inspection.
The embodiment of the present invention is given for the purpose of illustration and description, and is not exhaustively or by this to send out
It is bright to be limited to disclosed form.Many modifications and variations are obvious for the ordinary skill in the art.Choosing
Selecting and describe embodiment is and to make those skilled in the art to more preferably illustrate the principle of the present invention and practical application
It will be appreciated that the present invention is to design various embodiments suitable for specific applications with various modifications.
Claims (10)
1. a kind of device for subterranean pest-insect bioassay, which is characterized in that set relatively including bottom plate, two baffles and two groups
The side plate set, two baffles are respectively arranged at the both ends of the bottom plate, and side plate described in two groups is detachably arranged in respectively
The two sides of the bottom plate and each baffle；Side plate described in every group includes barn door and observation board, each barn door difference
Parallel is set to outside observation board.
2. the apparatus according to claim 1, which is characterized in that pass through respectively between the two sides and bottom of side plate described in two groups
Fixing component is removably correspondingly connected in the two sides of the bottom plate and each baffle, and the bottom plate and each baffle are all set in
Between two barn doors, and in the fixing component being overlapped between two barn door bottoms.
3. the apparatus of claim 2, which is characterized in that the fixing component includes screw rod and nut, the screw rod
Between side plate described in being set in two groups, one end outside weld of the screw rod is connected to the nut, the outer pivot bush unit one of the other end
A nut, two nuts are respectively arranged at outside side plate described in each group.
4. device according to claim 1-3, which is characterized in that two baffles are respectively fixedly disposed at institute
It states on the both ends of bottom plate, and the two sides of two baffles are flexibly connected with side plate described in two groups respectively.
5. device according to claim 1-3, which is characterized in that the bottom plate is equipped with one or more ventilative
Drain hole, when the ventilative drain hole is multiple, multiple ventilative drain holes are evenly distributed along the length direction of the bottom plate.
6. device according to claim 5, which is characterized in that the both ends of the bottom plate are removably connected with gear respectively
Plate, the two sides of two baffles are flexibly connected with side plate described in two groups respectively, and the bottom of the baffle and the bottom plate are removable
The connection unloaded.
7. device according to claim 6, which is characterized in that the bottom of each baffle is equipped with fixing bolt, institute
Fixing bolt is stated in any ventilative drain hole, and is fixed by locking nut, each baffle is detachable
Be set on any predeterminated position of the bottom plate, position tune is carried out between each predeterminated position by any baffle
It is whole, to change the spatial volume between the bottom plate, each baffle and two groups of side plates.
8. device according to claim 5, which is characterized in that the bottom plate passes through dog-ear connector and side described in any group
Plate detachable connection.
9. device according to claim 1-3, which is characterized in that the material of the barn door is non-transparent material
Light shield layer is coated on material or the barn door；The observation board is made of clear material.
10. a kind of method for carrying out subterranean pest-insect bioassay using the described in any item devices of such as claim 1-9, feature
It is, includes the following steps:
S1, immersion vegetable seeds to be measured are spare to after sprouting；
S2, assembling are used for the device of subterranean pest-insect bioassay, and described device includes that bottom plate, two baffles and two groups are oppositely arranged
Side plate, two baffles are respectively arranged at the both ends of the bottom plate, and side plate described in two groups is detachably arranged in institute respectively
State the two sides of bottom plate and each baffle；Side plate described in every group includes barn door and observation board, and each barn door is put down respectively
Capable is set to outside observation board；
S3, subterranean pest-insect biological test matrix is packed into described device, by the base in the seed implanted device after budding
In matter；
S4, each nut for being located at the same side in described device is unclamped, dismantles the side visor and retains observation board, planted with observing
Object root growth situation；
S5, when plant grows to suitable period, the side plate of described device either side is removed, by the underground of suitable age
Pest is by certain worm strain than reinstalling side plate described in the side in access described device；
After the completion of S6, the step S5, time recording plant strain growth parameter barn door described in removable side and retains institute therebetween
State observation board, observation of plant root growth and by Harm；After a period of time, side plate described in side is removed, removes plant to be measured
Object checks the root system victimization state to measuring plants, records related data；
S7, step S6 the data obtained is for statistical analysis, Harm of the comparison subterranean pest-insect to different plants.
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| CN207626200U (en) * | 2017-11-29 | 2018-07-20 | 中国农业科学院植物保护研究所 | Device for subterranean pest-insect bioassay |
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| US6253488B1 (en) * | 1996-11-04 | 2001-07-03 | Matthew Murfin | Two-part plant pot having a transparent internal plant-growing vessel |
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| CN204669973U (en) * | 2015-05-12 | 2015-09-30 | 北京农学院 | Plant root growth observation experiment planting container |
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