CN109821887B - In-situ anaerobic bioremediation method for contaminated soil of aeration zone - Google Patents
In-situ anaerobic bioremediation method for contaminated soil of aeration zone Download PDFInfo
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Abstract
The invention provides an in-situ anaerobic bioremediation method for polluted soil of an aeration zone, which comprises the steps of enabling the polluted soil of the aeration zone to be saturated with water by adopting a ground water seepage mode in a polluted area range, laying a remediation well, injecting nutrient substances into the remediation well or serving as an electron donor, and supplementing and injecting an electron donor such as sulfate or nitrate if necessary; in order to quickly achieve the repairing effect, domesticated anaerobic biological bacteria can be injected, nutrients, electron donors or anaerobic biological bacteria are quickly diffused into the polluted soil of the aeration zone within the repairing radius range of the repairing well by injecting compressed gas, the purpose of quickly repairing the polluted soil of the aeration zone by the anaerobic biological bacteria is achieved, and meanwhile, the repairable area can be clearly determined by arranging the repairing well.
Description
Technical Field
The invention relates to a soil and underground water pollution treatment technology, in particular to an in-situ anaerobic bioremediation method for aerated zone polluted soil.
Background
The aeration zone refers to the space part from the groundwater level to the ground surface. The polluted soil of the aeration zone can be excavated and subjected to ex-situ remediation such as incineration, thermal desorption, chemical treatment or biological remediation, but the remediation cost is higher, and particularly under the condition of deep pollution, the remediation cost is higher.
The soil bioremediation technology is a remediation technology for converting pollutants into nontoxic substances by means of the biodegradation of organic pollutants in soil by microorganisms. Microorganisms degrade organic pollutants by direct or co-metabolism to less toxic pollutants or completely to carbon dioxide and water. The in-situ bioremediation technology can be divided into an in-situ aerobic bioremediation technology and an in-situ anaerobic bioremediation technology according to aerobic or anaerobic characteristics of biological bacteria. Contaminants such as carbon tetrachloride, chloroform, tetrachloroethylene, and trichloroethylene are difficult to degrade by aerobic organisms, but can be degraded by anaerobic organisms.
Patent document CN100553809C provides a method for in situ microbial remediation of organic contaminated soil, which includes screening and establishing functional microbial groups, delivering functional microbes, and controlling remediation conditions in stages. The method prolongs the survival time of functional microorganism and improves the degradation rate of organic pollutants. The method mixes the medicament into the soil by adopting a method of disturbing the soil at a high frequency (80-130 times/day), and makes oxygen in the air enter the soil. The method belongs to in-situ aerobic bioremediation; the polluted soil is a solid phase, and the problem of uneven repair exists; high frequency mechanical disturbances are difficult to operate and the depth of contamination to be repaired is limited.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides an in-situ anaerobic bioremediation method for contaminated soil of an aeration zone. The bioremediation method comprises the steps of enabling the polluted soil of the aeration zone to be saturated with water by adopting a ground water seepage mode in the range of a polluted area, laying a remediation well, injecting nutrient substances into the remediation well or serving as an electron donor, and supplementing and injecting electron donors such as sulfate or nitrate and the like if necessary; in order to quickly achieve the restoration effect, domesticated anaerobic biological bacteria can be injected into the restoration well, nutrients, electron donors or anaerobic biological bacteria are quickly diffused into the polluted soil of the aeration zone in the restoration radius range of the restoration well by injecting compressed gas, the purpose of quickly restoring the polluted soil of the aeration zone by the anaerobic organisms is achieved, and meanwhile, the repairable area can be clearly determined by arranging the restoration well.
The purpose of the invention is realized by the following technical scheme:
an in-situ anaerobic bioremediation method for contaminated soil in an aeration zone is characterized by comprising the following steps:
1) the polluted soil of the aeration zone is saturated with water in a ground water seepage mode;
2) arranging a repairing well group in an area where water seepage on the ground is completed;
3) injecting nutrient substances into the repair well under pressure;
4) injecting the acclimated anaerobic biological bacteria of the soil target pollutants into the remediation well;
5) nutrient substances are injected into each restoration well in an intermittent small-dosage manner, so that the concentration of the nutrient substances in the soil saturated with water reaches more than 2mg/L, and the operation is continued until the soil reaches the standard.
The ground water seepage mode is as follows: vertically preventing seepage at the boundary of the site to be repaired to reach the underground water level; digging one or more seepage pits in a vertical seepage-proofing range, injecting clear water into the seepage pits, and maintaining a liquid level of 20-50 cm, so that the polluted soil in an aeration zone in a polluted area is saturated with water.
The depth of the repair well is consistent with the vertical seepage-proofing depth; the specification of the pit is as follows: 0.5 to 1.0m deep.
When the nutrient substances are injected under pressure in the repair well, sulfate or nitrate is also injected under pressure as an electron donor if necessary.
After injecting the substance in steps 3), 4) and 5), injecting gas through the repair well to diffuse the injected substance to the surrounding soil.
The nutrient is lactate, acetate, vegetable oil or molasses, and the nutrient can also be used as an electron donor; the gas is compressed nitrogen, argon or carbon dioxide.
The remediation well is used for pressurized injection of both nutrients and anaerobic organisms and for pressurized injection of gas.
The well group of the repair well is laid and comprises the following steps:
1a) determining a repair radius R;
1b) four repairing wells are arranged in a field to be repaired, so that a regular quadrangle with the side length of 2R is formed among the four repairing wells, and a repairing well is arranged in the center of each regular quadrangle;
1c) arranging other repair wells in sequence according to the method of the step 1 b) so that the intersection of all circles covers the site to be repaired, wherein the circle is a circle with the repair well as the center and the radius of the circle is the repair radius R.
Still include step 1 d) lay the monitoring well, lay the monitoring well and include: and monitoring wells are arranged on the tangency points of any two circles.
The method for determining the repair radius R comprises the following steps: a restoration well is built in a site to be restored, a plurality of monitoring wells are sequentially arranged at equal intervals of 1m around the restoration well, and after compressed nitrogen is continuously injected into the restoration well for 10-60 minutes, the dissolved nitrogen concentration of water in the monitoring wells can be increased to more than 1mg/L, and the distance from the monitoring well farthest from the restoration well is determined as a restoration radius R.
Compared with the prior art, the invention has the advantages that:
1) the technical scheme adopts a liquid-phase polluted soil in-situ anaerobic biological treatment method, and the liquid-phase bioremediation method using water as a medium has more uniform and more efficient remediation effect than the solid-phase bioremediation method;
2) the technical scheme adopts the repairing well which is used for injecting nutrient substances (or also used as an electron donor) such as lactate, acetate, vegetable oil or molasses and the like, an electron donor such as sulfate or nitrate and anaerobic biological bacteria under pressure, and also used for injecting inert gases such as compressed nitrogen, argon or carbon dioxide and the like under pressure to quickly diffuse the substances into the polluted soil around the repairing well. The anaerobic biological bacteria grow and propagate in large quantities to degrade pollutants, and the purpose of quickly restoring the polluted soil of the aeration zone is achieved. The repairable depth of the polluted soil of the aeration zone is not limited, and the operation is simple.
Drawings
The following is further described with reference to the accompanying drawings:
FIG. 1 is a schematic layout of a remediation well cluster for use in the method of the invention.
Detailed Description
The method and effect of the present invention will be further described in detail with reference to the accompanying drawings and specific embodiments.
An in-situ anaerobic bioremediation method for aerated zone contaminated soil, which adopts a remediation well to carry out remediation, comprises the following steps:
1) vertically preventing seepage at the boundary of the polluted area until the depth reaches the groundwater level;
2) digging a seepage pit with the depth of 0.5-1.0 m within the vertical seepage-proofing range;
3) injecting clear water into the infiltration pit, and maintaining the liquid level of about 20-50 cm, so that the polluted soil in the aeration zone in the polluted area is saturated with water;
4) and (3) constructing a repair well J1 in the site to be repaired, wherein the depth of the repair well is up to the vertical seepage-proofing depth. Monitoring wells are arranged at intervals of 1m, 2m, 3m. The depth of the monitoring well is consistent with the depth of the repairing well. After compressed nitrogen is continuously injected into the repair well J1 for 10-60 minutes, the distance from the monitoring well farthest from the repair well when the dissolved nitrogen in the water in the monitoring well can rise to more than 1mg/L is determined as a repair radius R;
5) and a repairing well group is distributed in the pit seepage area, and the depth of the repairing well is up to the vertical seepage-proofing depth. The method for laying the repair well is shown in figure 1, wherein the repair well J2 is laid at a position with a distance of 2R in any direction of the repair well J1, and a square is constructed by taking J1 and J2 as vertexes. Laying a repair well J7 and a repair well J8 at the other two vertex positions of the square; a repair well J4 is deployed in the center of this square. In fact, four repair wells J1, J2, J7 and J8 are centered, four circles with repair radius R are tangent, and the geometric center of the gap formed in the middle is the position of the fifth repair well J4;
6) and (4) sequentially laying other repairing wells according to the method, so that the intersection of circles taking the repairing wells as centers and the radius as the repairing radius covers the soil pollution area to be repaired. Monitoring wells can be arranged on the tangency points of any two circles, and the depth of each monitoring well is from the depth to the vertical seepage-proofing depth;
7) a certain amount of nutrients (or serving as electron donors) such as lactate, acetate, vegetable oil or molasses and the like are injected into each restoration well in a pressurizing mode by a water pump, and the nutrients are rapidly diffused into soil around the restoration well by injecting compressed nitrogen, argon or carbon dioxide into each restoration well. If necessary, a certain amount of electronic donors such as sulfate or nitrate are injected into each restoration well in a pressurizing way, and the sulfate or nitrate is quickly diffused into the soil around the restoration well by injecting compressed nitrogen, argon or carbon dioxide and other inert gases;
8) in order to quickly achieve the restoration effect, a water pump is adopted to pressurize and inject a certain amount of anaerobic biological agents into each restoration well, and compressed nitrogen, argon or carbon dioxide and other inert gases are injected into each restoration well to quickly diffuse the anaerobic biological agents into the soil around the restoration well. The anaerobic biological agent has good biodegradation effect on pollutants to be treated by pre-screening and domesticating;
9) each repairing well is indirectly injected with nutrients such as lactate, acetate, vegetable oil or molasses and the like (or also serves as an electron donor) in a small-dosage pressurizing manner, and the nutrients are rapidly diffused by injecting inert gases such as compressed nitrogen, argon or carbon dioxide and the like, so that the concentration of the nutrients in the saturated water of the soil reaches more than 2 mg/L;
10) the indigenous anaerobes in the polluted soil or the injected anaerobes grow in a large quantity to reproduce and degrade the pollutants to be treated until the polluted soil reaches the standard.
Specific examples are provided below
Example 1
1) Vertically preventing seepage at the boundary of the polluted area, wherein the depth is 6m, namely the buried depth of the groundwater level;
2) digging a penetration pit with the depth of 0.5m in the vertical seepage-proofing range;
3) injecting clear water in the vertical anti-seepage range, and maintaining the liquid level of about 20cm, so that the polluted soil in the aeration zone in the polluted area is saturated with water;
4) in a vertical seepage-proofing range, as shown in fig. 1, monitoring wells J1, J2 and J3. are arranged, J9, H1, H2, H3 and H4 are arranged, the repairing radius is 2m, and the depth of each repairing well and each monitoring well is 6 m;
5) after the J1 well was continuously injected with compressed nitrogen for 30 minutes, the dissolved nitrogen in the water in the H1 monitor well 2m away from the J1 well was allowed to rise to 1 mg/L;
6) injecting 80L of sodium lactate saturated solution into each restoration well by a water pump under pressure, continuously injecting compressed nitrogen for 30 minutes, and quickly diffusing the sodium lactate into the soil around the restoration wells;
7) injecting 100L of anaerobic biological bacteria liquid into each restoration well by adopting a water pump in a pressurizing way, continuously injecting compressed nitrogen for 30 minutes, and quickly diffusing the anaerobic biological bacteria liquid into the soil around the restoration well;
8) after continuously injecting a saturated solution of sodium lactate at a flow rate of 100L per hour for 30 minutes under pressure into each repair well, compressed nitrogen gas was continuously injected for 30 minutes, and then sodium lactate was injected under pressure again at intervals of 12 hours. By circulating in this way, the sodium lactate concentration in the water of the monitoring wells H1, H2, H3 and H4 is maintained to be more than 2 mg/L. Until the polluted soil reaches the standard.
Example 2
1) Vertically preventing seepage at the boundary of the polluted area, wherein the depth is 9m, namely the buried depth of the groundwater level;
2) digging a penetration pit with the depth of 0.5m in the vertical seepage-proofing range;
3) in the vertical anti-seepage range, injecting clear water, and maintaining the liquid level of about 30cm, so that the polluted soil in the aeration zone in the polluted area is saturated with water;
4) in a vertical seepage-proofing range, as shown in fig. 1, monitoring wells J1, J2 and J3. are arranged, J9, H1, H2, H3 and H4 are arranged, the repairing radius is 3m, and the depth of the repairing well and the monitoring well is 9 m;
5) after the J1 well was continuously injected with compressed nitrogen for 50 minutes, the dissolved nitrogen in the water in the H1 monitor well 3m away from the J1 well was allowed to rise to 1 mg/L;
6) injecting 120L of sodium lactate saturated solution into each restoration well by a water pump under pressure, and continuously injecting compressed nitrogen for 50 minutes to quickly diffuse sodium lactate into the soil around the restoration wells;
7) injecting 150L of anaerobic biological bacteria liquid into each restoration well by adopting a water pump in a pressurizing way, continuously injecting compressed nitrogen for 50 minutes, and quickly diffusing the anaerobic biological bacteria liquid into the soil around the restoration well;
8) after continuously injecting a sodium lactate saturated solution into each repair well at a flow rate of 200L per hour for 30 minutes under pressure, compressed nitrogen is continuously injected for 50 minutes, and then sodium lactate is injected under pressure after 12 hours of intermission. By circulating in this way, the sodium lactate concentration in the water of the monitoring wells H1, H2, H3 and H4 is maintained to be more than 2 mg/L. Until the polluted soil reaches the standard.
Although the invention has been described in detail hereinabove by way of general description, specific embodiments and experiments, it will be apparent to those skilled in the art that many modifications and improvements can be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (7)
1. An in-situ anaerobic bioremediation method for contaminated soil in an aeration zone is characterized by comprising the following steps:
1) the polluted soil of the aeration zone is saturated with water in a ground water seepage mode;
2) arranging a repairing well group in an area where water seepage on the ground is completed;
3) injecting nutrient substances into the repair well under pressure;
4) injecting the acclimated anaerobic biological bacteria of the soil target pollutants into the remediation well;
5) nutrient substances are injected into each repairing well in an intermittent small-dosage manner, so that the concentration of the nutrient substances with full water in the soil reaches more than 2mg/L, and the operation is continued until the soil reaches the standard;
injecting nutrient substances in the steps 3) and 5), injecting gas through the remediation well after injecting anaerobic biological bacteria in the step 4), and enabling the injected nutrient substances and the anaerobic biological bacteria to diffuse to the surrounding soil, wherein the gas is compressed nitrogen;
the well group for laying and repairing comprises the following steps:
1a) determining a repair radius R;
1b) four repairing wells are distributed in a site to be repaired, a regular quadrangle with the side length of 2R is formed among the four repairing wells, and a repairing well is arranged in the center of the regular quadrangle;
1c) arranging other repair wells successively according to the method of the step 1 b) to enable the intersection of all circles to cover the site to be repaired, wherein the circles are circles taking the repair wells as centers and the radius of the repair circles as the repair radius R;
the method for determining the repair radius R comprises the following steps: a restoration well is built in a site to be restored, a plurality of monitoring wells are sequentially arranged at equal intervals of 1m around the restoration well, and after compressed nitrogen is continuously injected into the restoration well for 10-60 minutes, the dissolved nitrogen concentration of water in the monitoring wells can be increased to more than 1mg/L, and the distance from the monitoring well farthest from the restoration well is determined as a restoration radius R.
2. The in-situ anaerobic bioremediation method for the aerated zone contaminated soil according to claim 1, wherein the ground water seepage mode is: vertically preventing seepage at the boundary of the site to be repaired to reach the underground water level; digging one or more seepage pits in a vertical seepage-proofing range, injecting clear water into the seepage pits, and maintaining a liquid level of 20-50 cm, so that the polluted soil in an aeration zone in a polluted area is saturated with water.
3. The in-situ anaerobic bioremediation method for aerated zone contaminated soil according to claim 2, wherein the depth of said remediation well is consistent with the vertical seepage control depth; the specification of the pit is as follows: 0.5 to 1.0m deep.
4. The in-situ anaerobic bioremediation method of aerated contaminated soil according to claim 1, wherein sulfate or nitrate is further pressure injected as an electron donor when the nutrient is pressure injected into the remediation well.
5. The in situ anaerobic bioremediation method of aerated contaminated soil according to claim 4, wherein said nutrients are lactate, acetate, vegetable oil or molasses.
6. The in situ anaerobic bioremediation method of aerated contaminated soil according to claim 1, wherein said remediation wells are used for both pressurized injection of nutrients and anaerobic organisms and pressurized injection of gas.
7. The in-situ anaerobic bioremediation method of the gas-enclosed zone contaminated soil according to claim 1, further comprising the step of 1 d) laying monitoring wells, wherein the laying of the monitoring wells comprises: and monitoring wells are arranged on the tangency points of any two circles.
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| CN111250538A (en) * | 2020-01-17 | 2020-06-09 | 轻工业环境保护研究所 | Method for restoring gasoline-polluted site soil |
| CN115232625A (en) * | 2021-09-30 | 2022-10-25 | 黄芸 | Treatment method and application of soil organic pollutants |
| CN114226449B (en) * | 2021-11-29 | 2023-05-02 | 辽宁中博生态环境技术有限公司 | Soil gas-phase diffusion restoration treatment method for volatile organic contaminated soil |
| CN114850201B (en) * | 2022-05-26 | 2024-02-06 | 北京博诚立新环境科技股份有限公司 | In-situ bioremediation method for combined treatment of gas-coated belt and saturated belt |
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| US20110207204A1 (en) * | 2010-02-19 | 2011-08-25 | Tommy Mack Davis | Method and apparatus for bioremediation of soils and sediments |
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| WO2005118171A1 (en) * | 2004-06-02 | 2005-12-15 | Ecocycle Corporation | Additive for use to restore polluted soil, groundwater or bottom sediment soil |
| JP2012040476A (en) * | 2010-08-16 | 2012-03-01 | Shimizu Corp | Method and device for purifying contaminated soil or groundwater |
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