CN109820789A - A kind of preparation method and facial mask liquid of the bear's weed facial mask annex solution of five bacterium fermentation - Google Patents
A kind of preparation method and facial mask liquid of the bear's weed facial mask annex solution of five bacterium fermentation Download PDFInfo
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- CN109820789A CN109820789A CN201910294212.8A CN201910294212A CN109820789A CN 109820789 A CN109820789 A CN 109820789A CN 201910294212 A CN201910294212 A CN 201910294212A CN 109820789 A CN109820789 A CN 109820789A
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Abstract
The present invention relates to cosmetic technical field, the specifically a kind of preparation method and facial mask liquid of the bear's weed facial mask annex solution of five bacterium fermentation, it is characterised in that use following steps: obtaining primary fermentation substrate after bear's weed powder is mixed with deionized water；Aerobic fermentation is carried out to primary fermentation substrate using bacillus subtilis, brewer's yeast and yellow wine yeast mixed bacteria liquid and obtains secondary fermentation substrate；Anaerobic fermentation is carried out to secondary fermentation substrate using Bacillus acidi lactici and the mixed bacteria liquid of Bifidobacterium, centrifuging and taking supernatant obtains facial mask annex solution.Compared with prior art, the present invention combining unique fermentation method using a variety of zymophytes, five fermented liquids of acquisition remain the whole functional components and its activity of bear's weed, avoid the loss of active constituent caused by extracting method；Fermentation temperature and fermentation pH are mild, and active components of plants structure is not destroyed, and keep the natural activity of plant, and it is easy to operate, cost is relatively low, public hazards are few.
Description
Technical field
The present invention relates to cosmetic technical field, specifically a kind of bear's weed facial mask annex solution of five bacterium fermentation
Preparation method and facial mask liquid.
Background technique
With microbial fermentation technology fermenting plant etc., microorganism extracts or mycetocyte object etc. are applied in cosmetics,
It is the research hotspot of current cosmetics both at home and abroad.Microbial fermentation technology can be thin with complete microbial cell or from microorganism
The enzyme extracted in born of the same parents as biocatalyst, regio- and stereo-selectivity is strong, reaction condition is mild, easy to operate, cost compared with
It is low, public hazards are few, the very big attention by every field.
Summary of the invention
It is an object of the present invention to ferment using multiple-microorganism to bear's weed, and cooperate unique fermentation hand
Section, does not add any organic reagent, mild to ferment, and greatly retains the whole functional components and activity of bear's weed, avoids activity
Ingredients from lossing guarantees the natural activity of plant.
To achieve the above object, a kind of preparation method of the bear's weed facial mask annex solution of five bacterium fermentation, feature are designed
It is using following steps:
(1) it obtains primary fermentation substrate: after bear's weed powder is mixed with deionized water, carrying out first time high temperature and go out
Bacterium, cooling obtain primary fermentation substrate；
(2) aerobic fermentation prepares secondary fermentation substrate: being had with zymophyte to primary fermentation substrate using aerobic fermentation
After aerobe fermentation, second of high-temperature sterilization, cooling are carried out, obtains secondary fermentation substrate；The aerobic fermentation is withered grass with zymophyte
Bacillus, brewer's yeast and yellow wine yeast；
(3) anaerobic fermentation obtains five fermented liquids: carrying out anaerobism to secondary fermentation substrate with zymophyte using anaerobic fermentation
After fermentation, then third time high-temperature sterilization, cooling are carried out, obtains five fermented liquids；
The anaerobic fermentation is Bacillus acidi lactici and Bifidobacterium with zymophyte；
(4) it is centrifuged: after being centrifuged to five fermented liquids, taking supernatant, obtain facial mask annex solution.
Further, the mesh number of the bear's weed powder is 20~50 mesh.
Further, the bear's weed powder: the solid-liquid ratio of deionized water is 1g: 10ml~1g: 20ml.
Further, the aerobic fermentation are as follows: the aerobic fermentation that total volume is 10~50ml is added to 100 with zymophyte
In~500ml primary fermentation substrate, with 25~32 DEG C of temperature, shaking speed is 100~200r/min aerobic fermentation 36~48
Hour；
The aerobic fermentation is with bacillus subtilis in zymophyte: brewer's yeast: the volume ratio of yellow wine yeast is 1: 1: 1；
The bacillus subtilis, brewer's yeast, yellow wine yeast concentration be respectively 105~108CFU/ml。
Further, the first time high-temperature sterilization is with 105~115 DEG C, and sterilize 40~60min.
Further, second of high-temperature sterilization is with 100~121 DEG C, and sterilize 20min~60min.
Further, the third time high-temperature sterilization is with 115~121 DEG C, and sterilize 20min~60min.
Further, the anaerobic fermentation are as follows: the anaerobic fermentation that total volume is 20~100ml is added with zymophyte
In the secondary fermentation substrate of 100~500ml, with 38~45 DEG C anaerobic fermentation 6~15 hours；
Anaerobic fermentation Bacillus acidi lactici in zymophyte: volume ratio=1 of Bifidobacterium: 1；
The Bacillus acidi lactici, Bifidobacterium concentration be respectively 105~108CFU/ml。
Further, the revolving speed of the centrifugation is 4000~10000r/r/min, and centrifugation time is 10~60min.
Further, the main component that five fermented liquid includes are as follows: Bacillus acidi lactici/bear's weed tunning extracts
Object, two split yeast tunning lysis object, bacillus subtilis enzyme, saccharomycete lysis object, brewer's yeast fungus extract.
Facial mask liquid containing the facial mask annex solution.
Further, the adding proportion of the facial mask annex solution is 0.1~100wt%.
Compared with prior art, the present invention having the advantage that
(1) present invention ferments to bear's weed using microorganism, remain bear's weed whole functional components and
Its activity avoids the loss of active constituent caused by extracting method；
(2) fermentation method of the present invention does not add any organic in bear's weed effective component extraction process
Reagent, fermentation temperature and fermentation pH are mild, and active components of plants structure is not destroyed, and keeps the natural activity of plant, and grasp
Work is easy, cost is relatively low, and public hazards are few；
(3) bacillus subtilis that the present invention uses has stronger amylase, proteinase；Using
Saccharomycete, have that mycoprotein content is high, amino acid composition rationally and can generate the characteristics such as B family vitamin；It uses
Carbohydate metabolism in substrate can be during the fermentation lactic acid, adjust pH value, while making to produce by Bifidobacterium and Bacillus acidi lactici
Product have aromatic odor.
(4) present invention uses five bacterium cooperative cooperatings, sufficiently extracts the effective component in substrate, and generate new active matter
Matter, five fermented liquids and facial mask liquid made all have higher skin-care effect.
Specific embodiment
The present invention is further described now in conjunction with embodiment.But it should not be construed as limiting the invention, do not carrying on the back
In the case where from spirit of that invention and essence, to modifications or substitutions made by the method for the present invention, step or condition, this hair is belonged to
Bright range.
Unless otherwise specified, the conventional means that technological means used in embodiment is well known to those skilled in the art.
Embodiment 1
One, the preparation of five fermented liquids
According to microorganism routine culture, bacillus subtilis, yellow wine yeast, brewer's yeast, Bifidobacterium, lactic acid bar are cultivated
Bacterium, making above-mentioned bacterial concentration is respectively 107CFU/ml。
Bear's weed is carried out to be crushed to 50 mesh, takes 30g bear's weed powder to be placed in the triangular glass bottle of 500ml, adds
Enter 300ml deionized water, after sealed membrane sealing, 115 DEG C, sterilize 40min.Primary fermentation substrate is obtained after standing cooling.
Access in primary fermentation substrate bacillus subtilis, yellow wine yeast and brewer's yeast mix in equal volume
Anaerobic fermentation is 30ml with zymogenic bacterium solution total volume with zymophyte, the aerobic fermentation.It is arranged 28 DEG C in shaking table, 180r/r/
42 hours progress aerobic fermentations are cultivated under the conditions of min.After aerobic fermentation by fermentation liquid at 121 DEG C, sterilize 30min, quiet
Secondary fermentation substrate is obtained after setting cooling.
Bifidobacterium is accessed in secondary fermentation substrate to ferment with the anaerobic fermentation that Bacillus acidi lactici mixes in equal volume
Bacterium, the anaerobic fermentation are 30ml with zymogenic bacterium solution total volume.It cultivates 10 hours under the conditions of 40 DEG C and is detested in the incubator
Aerobe fermentation.After anaerobic fermentation by fermentation liquid at 121 DEG C, after the 30min that sterilizes, five fermented liquids are obtained, five bacterium are fermented
Liquid is centrifuged 60min with 4000r/r/min, takes supernatant, obtains facial mask annex solution.
Two, the preparation of the facial mask liquid of five fermented liquids containing 50wt%
The facial mask annex solution for taking the above-mentioned preparation of 50g, with 5g glycerol, 5g butanediol, 1g Butyrospermum parkii fruit rouge, 1g phytosterol
Class, 1g cetostearyl alcohol, 1g cyclohexasiloxane, 0.5g stearine, 0.5g PEG-100 stearate, 1g ripple angle fork
Dish extract, 5g biology carbohydrate gum, 1g beta glucan, 1g Gotu Kola P.E, 1g polygonum cuspidate root extract, the extraction of 1g scutellariae,radix, 1g
Tea extract, 1g glycyrrhiza glabra root extract, 1g german chamomile flower extract, 1g rosemary leaf extract, 1g hydrolysis placenta (sheep)
Extract, 2g Sodium Hyaluronate, 5g 1,2- hexylene glycol, 2g decoyl hydroximic acid, 5g 1,3-PD, 5g hexylene glycol, 1g dent
Amaranth extract, after mixing evenly up to a kind of facial mask liquid containing five fermented liquid of 50wt%.
Above embodiments prepare that the appearance of the resulting facial mask liquid containing five fermented liquids is thick liquid, color is light powder
Color is to brownish red.PH value 5.2-6.8, viscosity 200-500cP, soluble solids content 1.5-5.0%, total plate count are less than
50CFU/ml, no pathogenic bacteria detection.Meet cosmetics quality requirement.
Embodiment 2
One, the preparation of five fermented liquids
According to microorganism routine culture, bacillus subtilis, yellow wine yeast, brewer's yeast, Bifidobacterium, lactic acid bar are cultivated
Bacterium, making the concentration of above-mentioned bacterium solution is respectively 106CFU/ml。
Bear's weed is carried out to be crushed to 40 mesh, takes 40g bear's weed powder to be placed in the triangular glass bottle of 500ml, adds
Enter 400ml deionized water, after sealed membrane sealing, 105 DEG C, sterilize 60min.Primary fermentation substrate is obtained after standing cooling.
Access in primary fermentation substrate bacillus subtilis, yellow wine yeast and brewer's yeast mix in equal volume
Aerobic fermentation is 50ml with zymogenic total volume with zymophyte, the aerobic fermentation.It is arranged 30 DEG C in shaking table, 150r/min condition
48 hours progress aerobic fermentations of lower culture.After aerobic fermentation by fermentation liquid at 115 DEG C, sterilize 40min, after standing cooling
Obtain secondary fermentation substrate.
Bifidobacterium is accessed in secondary fermentation substrate to ferment with the anaerobic fermentation that Bacillus acidi lactici mixes in equal volume
Bacterium, the anaerobic fermentation are 50ml with zymogenic total volume.8 hours progress anaerobism hairs are cultivated under the conditions of 42 DEG C in the incubator
Ferment.After anaerobic fermentation by fermentation liquid at 115 DEG C, sterilize 40min after, obtain five fermented liquids, by five fermented liquids with
6000r/min is centrifuged 40min, takes supernatant, obtains facial mask annex solution.
Two, the preparation of the facial mask liquid of five fermented liquids containing 98wt%
Addition 1% hexylene glycol, 1% propylene glycol in resulting facial mask annex solution is prepared by above-mentioned, is after mixing evenly
Obtain a kind of facial mask liquid of five fermented liquid containing 98wt%.
Above embodiments prepare that the appearance of the resulting facial mask liquid for containing five fermented liquids is thick liquid, color is pale pink
To brownish red.PH value 5.2-6.8, viscosity 200-500cP, soluble solids content 1.5-5.0%, total plate count are less than
50CFU/ml, no pathogenic bacteria detection.Meet cosmetics quality requirement.
Design principle of the invention is: microorganism can generate protease, amylase, cellulase etc. during the fermentation and disappear
Change enzyme, the anti-nutritional factors in fermentation substrate can be eliminated, degradation Large molecule active substance makes its molecular weight be lower.By a variety of
Microorganism jointly uniquely ferments to substrate, and different strains is made to have oneself unique performance, such as lichens gemma bar
Bacterium and bacillus subtilis have stronger amylase, proteinase；Saccharomycete mycoprotein content height, amino
Acid composition is reasonable, and B family vitamin can be generated etc.；Carbohydate metabolism can be during the fermentation lactic acid by lactic acid bacteria, be adjusted
Product pH, while making product that there is aromatic odor.
Claims (12)
1. a kind of preparation method of the bear's weed facial mask annex solution of five bacterium fermentation, it is characterised in that use following steps:
(1) primary fermentation substrate is obtained: after bear's weed powder is mixed with deionized water, progress first time high-temperature sterilization, cold
But, primary fermentation substrate is obtained；
(2) aerobic fermentation prepares secondary fermentation substrate: carrying out aerobic hair to primary fermentation substrate with zymophyte using aerobic fermentation
After ferment, second of high-temperature sterilization, cooling are carried out, obtains secondary fermentation substrate；The aerobic fermentation is withered grass gemma with zymophyte
Bacillus, brewer's yeast and yellow wine yeast；
(3) anaerobic fermentation obtains five fermented liquids: carrying out anaerobic fermentation to secondary fermentation substrate with zymophyte using anaerobic fermentation
Afterwards, then third time high-temperature sterilization, cooling are carried out, obtains five fermented liquids；The anaerobic fermentation with zymophyte be Bacillus acidi lactici and
Bifidobacterium；
(4) it is centrifuged: after being centrifuged to five fermented liquids, taking supernatant, obtain facial mask annex solution.
2. the preparation method of the bear's weed facial mask annex solution of one kind as described in claim 1 five bacterium fermentation, which is characterized in that
The mesh number of the bear's weed powder is 20~50 mesh.
3. the preparation method of the bear's weed facial mask annex solution of one kind as described in claim 1 five bacterium fermentation, which is characterized in that
The bear's weed powder: the solid-liquid ratio of deionized water is 1g: 5ml~1g: 20ml.
4. the preparation method of the bear's weed facial mask annex solution of one kind as described in claim 1 five bacterium fermentation, which is characterized in that
The aerobic fermentation are as follows: the aerobic fermentation that total volume is 10~50ml is added to 100~500ml primary hair with zymophyte
In ferment substrate, with 25~32 DEG C of temperature, shaking speed is 100~200r/min aerobic fermentation 36~48 hours；
The aerobic fermentation is with bacillus subtilis in zymophyte: brewer's yeast: the volume ratio of yellow wine yeast is 1: 1: 1；
The bacillus subtilis, brewer's yeast, yellow wine yeast concentration be respectively 105~108CFU/ml。
5. the preparation method of the bear's weed facial mask annex solution of one kind as described in claim 1 five bacterium fermentation, which is characterized in that
The first time high-temperature sterilization is with 105~115 DEG C, and sterilize 40~60min.
6. the preparation method of the bear's weed facial mask annex solution of one kind as described in claim 1 five bacterium fermentation, which is characterized in that
Second of high-temperature sterilization is with 100~121 DEG C, and sterilize 20min~60min.
7. the preparation method of the bear's weed facial mask annex solution of one kind as described in claim 1 five bacterium fermentation, which is characterized in that
The third time high-temperature sterilization is with 115~121 DEG C, and sterilize 20min~60min.
8. the preparation method of the bear's weed facial mask annex solution of one kind as described in claim 1 five bacterium fermentation, which is characterized in that
The anaerobic fermentation are as follows: the secondary of 100~500ml is added with zymophyte in the anaerobic fermentation that total volume is 20~100ml
Grade fermentation substrate in, with 38~45 DEG C anaerobic fermentation 6~15 hours；
Anaerobic fermentation Bacillus acidi lactici in zymophyte: volume ratio=1 of Bifidobacterium: 1；
The Bacillus acidi lactici, Bifidobacterium concentration be respectively 105~108CFU/ml。
9. the preparation method of the bear's weed facial mask annex solution of one kind as described in claim 1 five bacterium fermentation, which is characterized in that
The revolving speed of the centrifugation is 4000~10000r/min, and centrifugation time is 10~60min.
10. the preparation method of the bear's weed facial mask annex solution of one kind as described in claim 1 five bacterium fermentation, feature exist
In the main component that five fermented liquid includes are as follows: Bacillus acidi lactici/bear's weed tunning extract, two split yeast hair
Ferment product lysis object, bacillus subtilis enzyme, saccharomycete lysis object, brewer's yeast fungus extract.
11. the facial mask liquid containing the facial mask annex solution as described in any one of claim 1~10.
12. facial mask liquid as claimed in claim 11, which is characterized in that the adding proportion of the facial mask annex solution be 0.1~
100wt%.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110403872A (en) * | 2019-08-29 | 2019-11-05 | 湖北省麦诗特生物科技有限公司 | A kind of preparation method and application of the bear's weed tunning extract with moisture-keeping efficacy |
| CN115554226A (en) * | 2022-11-11 | 2023-01-03 | 广州优科生物科技有限公司 | Multi-strain fermentation filtrate and preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106333921A (en) * | 2016-08-26 | 2017-01-18 | 施协均 | Milky mask based on viable yeast and plant stem cells and preparation method thereof |
| CN109329319A (en) * | 2018-11-23 | 2019-02-15 | 刘武 | The preparation method of herb fermenting mosquito repellent liquid, its stoste and dilution |
-
2019
- 2019-04-12 CN CN201910294212.8A patent/CN109820789A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106333921A (en) * | 2016-08-26 | 2017-01-18 | 施协均 | Milky mask based on viable yeast and plant stem cells and preparation method thereof |
| CN109329319A (en) * | 2018-11-23 | 2019-02-15 | 刘武 | The preparation method of herb fermenting mosquito repellent liquid, its stoste and dilution |
Non-Patent Citations (1)
| Title |
|---|
| 上海泰旎生物科技有限公司: "TST五菌合一逆龄修护面膜乳", 《国产非特殊用途化妆品备案信息服务平台》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110403872A (en) * | 2019-08-29 | 2019-11-05 | 湖北省麦诗特生物科技有限公司 | A kind of preparation method and application of the bear's weed tunning extract with moisture-keeping efficacy |
| CN115554226A (en) * | 2022-11-11 | 2023-01-03 | 广州优科生物科技有限公司 | Multi-strain fermentation filtrate and preparation method and application thereof |
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