CN109811580B - Biological paper pulp taking peanut shells and reed as raw materials and preparation method and application thereof - Google Patents
Biological paper pulp taking peanut shells and reed as raw materials and preparation method and application thereof Download PDFInfo
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- CN109811580B CN109811580B CN201910189396.1A CN201910189396A CN109811580B CN 109811580 B CN109811580 B CN 109811580B CN 201910189396 A CN201910189396 A CN 201910189396A CN 109811580 B CN109811580 B CN 109811580B
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Abstract
The invention relates to a preparation method of biological paper pulp by taking peanut shells and reeds as raw materials, which comprises the following steps: inoculating TrichodermaInoculating the cultured seed liquid into a culture medium; mixing peanut shells and reeds and then sterilizing; adding the strain into the product, soaking, filtering to remove bacterial liquid, pulping the soaked reed and peanut shells, washing the pulp, and drying to obtain dry paper pulp; h is to be2O2Mixing with NaOH, bleaching dry paper pulp with mixed liquor, bleaching with mixed enzyme liquor, carrying out suction filtration, flattening filter residue and drying. The preparation method of the invention has the advantages of short growth cycle of the used raw materials, rich yield, capability of reducing cost and saving forest resources when used for pulping and papermaking, and contribution to environmental protection. The raw material is soaked by trichoderma to degrade lignin in the raw material, the generation of cooking black liquor is reduced, and laccase and xylanase are used for bleaching paper pulp in an assistant manner, so that the using amount of a chemical bleaching agent is reduced, and the whiteness of the paper is increased.
Description
Technical Field
The invention belongs to a method for making paper by degrading non-wood fibers with microorganisms, and particularly relates to biological paper pulp taking peanut shells and reeds as raw materials, and a preparation method and application thereof.
Background
The paper making raw materials comprise plant fibers and non-plant fibers, the paper making materials of the non-plant fibers comprise a plurality of inorganic fibers, microbial fibers and the like, the plant fibers can be subdivided into wood fibers and non-wood fibers, the wood fibers have high utilization rate in China and cause serious environmental pollution, so the development of the non-wood fibers as the paper making raw materials is more important.
China is a world-wide large peanut producing country, 500 million tons of peanut shells are produced every year, at present, only a few peanut shells are used for animal feed, and most of the peanut shells are thrown away or burnt as garbage wastes. The peanut shells contain a large amount of natural high molecular substances such as cellulose, lignin, hemicellulose and the like, the content of the cellulose in the peanut shells is 43.84%, and the peanut shells are only short in fiber and can also become a good papermaking raw material when being mixed with other raw materials for use. The reed is a widely distributed gramineae perennial herbaceous plant, mainly grows in wetland environment, and exists in large amount in rivers, lakes and other regions with much water. The reed is abundant in reed resources in China, and mainly produced in inner Mongolia, Xinjiang, northeast and other places. The reed has straight, tall and tough stem and high fiber content, contains 44 percent of cellulose, is second only to cotton and hemp, and is an ideal pulping and papermaking raw material. The reed and the peanut shell are mixed to be used as raw materials for papermaking, so that not only can waste be utilized, but also forest resources can be saved.
The traditional pulping method not only consumes a large amount of chemicals and energy, but also produces waste liquid which causes serious pollution to the environment. The adoption of biological pulping and biological bleaching can reduce the use amount of chemical reagents and reduce the pollution to the environment when the paper pulp is bleached.
Disclosure of Invention
The invention aims to provide a preparation method of biological paper pulp by taking peanut shells and reeds as raw materials.
The invention relates to a preparation method of biological paper pulp by taking peanut shells and reeds as raw materials, which comprises the following steps: s101, inoculating 3-5% of trichoderma in 200-300 mL of potato glucose liquid culture medium according to the inoculation amount, performing shake culture for 2-3 days, and inoculating 3-5% of the inoculation amount of the cultured seed liquid in 0.8-1.2L of potato glucose liquid culture medium for culture for 2-4 days to reach a logarithmic growth phase; s102: cutting peanut shell into 3mm2～5mm2The small pieces and the reeds are cut into small sections of 1-2 cm, and then the small sections and the reeds are mixed according to the mass ratio of 1: 1-4 and then are sterilized; s103: adding the strains cultured in the step S101 into the product obtained in the step S102, soaking for 8-10 days, filtering out bacterial liquid, pulping the soaked reed and peanut shells, washing the pulp, and drying to obtain dry paper pulp; s104: 10 mL-20 mL of H with the mass fraction of 30 percent2O2Mixing the dry paper pulp with 5g to 10g of NaOH to obtain mixed solution, then weighing 5g to 10g of the dry paper pulp, bleaching the dry paper pulp by using the mixed solution, and then bleaching the dry paper pulp by using the mixed solution according to the mass ratio of 1: (1-9) bleaching the mixed enzyme solution of the laccase and the xylanase, performing suction filtration, flattening filter residues, and drying to obtain the peanut shells and the reed serving as raw materialsBiological pulp.
The method for preparing the biological paper pulp by using the peanut shells and the reeds as the raw materials has the advantages that the used raw materials of the peanut shells and the reeds are short in growth cycle and rich in yield, and the method is used for pulping and papermaking, can reduce cost, saves forest resources and is beneficial to environmental protection. Meanwhile, the values of the peanut shells and the reeds are improved. The raw material is soaked by the trichoderma to degrade lignin in the raw material, the generation of the cooking black liquor is reduced, and the laccase and the xylanase are used for bleaching the paper pulp in an assistant manner, so that the using amount of a chemical bleaching agent is reduced, and the whiteness of the paper is increased.
In addition, the preparation method of the biological paper pulp taking the peanut shells and the reeds as the raw materials can also have the following additional technical characteristics:
further, after bleaching in step S104, the fabric is washed with clean water 2 to 3 times.
Further, in the step S102, ultraviolet sterilization is used for 25 to 35min during the sterilization treatment.
Further, in the step S101, the temperature of the shake cultivation is 28 ℃ to 32 ℃, and the rotation speed is 140rpm to 160 rpm.
Further, in the step S104, the drying temperature is 60 ℃ to 80 ℃, and the drying time is 1h to 2 h.
Further, in the step S104, the temperature for bleaching with the mixed enzyme solution of laccase and xylanase is 40 ℃ to 50 ℃, and the mixture is stirred for 1.5h to 2h when bleaching is carried out at the temperature.
Another object of the invention is to propose a biopaper prepared by said method.
It is a further object of the invention to propose the use of said bio-pulp in papermaking.
Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.
Drawings
FIG. 1 is a schematic representation of the enzymatic activity of the laccase of the invention;
FIG. 2 is a schematic diagram showing the enzymatic activities of the xylanases of the invention.
Detailed Description
The following detailed description of embodiments of the invention is intended to be illustrative, and not to be construed as limiting the invention.
Example 1
(1) inoculating trichoderma in an inoculum size of 3% by mass into 300mL of potato glucose liquid culture medium, then carrying out shake culture at the temperature of 28 ℃ and the rotating speed of 160rpm for 2 days, and then inoculating the cultured seed liquid in a potato glucose liquid culture medium of 0.8L by an inoculum size of 5% by mass for culture for 4 days so as to reach the logarithmic phase.
(2) Cutting peanut shell into 3mm2The small pieces and the reeds are cut into small pieces of 2cm, and then the weight ratio of the peanut shells to the reeds is 1: 1 mixing, and ultraviolet sterilizing for 25 min.
(3) Adding the strains cultured in the step (1) into the product obtained in the step (2) and soaking for 10 days, then filtering out bacterial liquid, pulping the soaked reed and peanut shells, washing the pulp and drying to obtain dry paper pulp.
(4) 10mL of H with the mass fraction of 30 percent2O2Mixing the dry paper pulp with 10g of NaOH to obtain a mixed solution, weighing 5g of the dry paper pulp, bleaching the dry paper pulp by using the mixed solution, and then bleaching the dry paper pulp by using a mixed solution with a mass ratio of 1: 9 stirring the mixed enzyme solution of the laccase and the xylanase mixed at the temperature of 40 ℃ for 2 hours for bleaching, then washing for 2 times by using clear water, then carrying out suction filtration, flattening filter residues, and then drying at the temperature of 80 ℃ for 1 hour to obtain the biological paper pulp taking the peanut shells and the reeds as raw materials.
Example 2
(1) inoculating trichoderma in 200mL of potato glucose liquid culture medium according to the inoculation amount with the mass fraction of 5%, then carrying out shake culture at the temperature of 32 ℃ and the rotating speed of 140rpm for 3 days, and then inoculating the cultured seed liquid in 1.2L of potato glucose liquid culture medium according to the inoculation amount with the mass fraction of 3% for 2 days so as to reach the logarithmic phase.
(2) Cutting peanut shell into 5mm2The small pieces and the reed are cut into small sections of 1cm, then the peanut shell and the reed are mixed according to the mass ratio of 1: 4, and then the mixture is subjected to ultraviolet sterilization for 35 min.
(3) Adding the strains cultured in the step (1) into the product obtained in the step (2) and soaking for 8 days, then filtering out bacterial liquid, pulping the soaked reed and peanut shells, washing the pulp and drying to obtain dry paper pulp.
(4) 20mL of H with the mass fraction of 30 percent2O2Mixing the dry paper pulp with 5g of NaOH to obtain a mixed solution, weighing 10g of the dry paper pulp, bleaching the dry paper pulp by using the mixed solution, and then bleaching the dry paper pulp by using a mixed solution with a mass ratio of 1: 1, stirring the mixed enzyme solution of the laccase and the xylanase mixed at the temperature of 50 ℃ for 1.5 hours for bleaching, then washing for 3 times by using clear water, then carrying out suction filtration, flattening filter residues, and then drying at the temperature of 60 ℃ for 2 hours to obtain the biological paper pulp taking peanut shells and reeds as raw materials.
Example 3
(1) inoculating trichoderma in an inoculum size of 4% by mass into 250mL of potato glucose liquid culture medium, then carrying out shake culture at the temperature of 30 ℃ and the rotating speed of 150rpm for 2 days, and then inoculating the cultured seed liquid in a 1L of potato glucose liquid culture medium for 3 days according to the inoculum size of 4% by mass so as to reach the logarithmic phase.
(2) Cutting peanut shell into 4mm2The small pieces and the reed are cut into small sections of 1cm, then the peanut shell and the reed are mixed according to the mass ratio of 1: 2, and then the mixture is subjected to ultraviolet sterilization for 30 min.
(3) Adding the strains cultured in the step (1) into the product obtained in the step (2) and soaking for 9 days, then filtering out bacterial liquid, pulping the soaked reed and peanut shells, washing the pulp and drying to obtain dry paper pulp.
(4) 15mL of H with the mass fraction of 30 percent2O2Mixing the dry paper pulp with 7g of NaOH to obtain a mixed solution, weighing 7g of the dry paper pulp, bleaching the dry paper pulp by using the mixed solution, and then bleaching the dry paper pulp by using a mixed solution with a mass ratio of 1: 5 stirring the mixed enzyme solution of the laccase and the xylanase mixed at the temperature of 45 ℃ for 1.8h for bleaching, washing for 2 times by using clear water, then carrying out suction filtration, flattening filter residues, and drying at the temperature of 70 ℃ for 2h to obtain the biological paper pulp taking the peanut shells and the reeds as raw materials.
Example 4
inoculating trichoderma strain seed liquid into a 300mLPDA culture medium according to the inoculation amount of 3-5% by mass, then carrying out shake cultivation for 3 days at 30 ℃ and 150rpm, mixing 5g of raw materials according to the proportion of 1: 3 of peanut shells and bulrushes, sterilizing, adding the raw materials into the culture solution, soaking for 8 days, filtering to remove bacterial liquid, pulping the soaked bulrushes and peanut shells, washing the pulp, and drying. First, 10ml of 30% H is used2O2+5g NaOH bleached, pressed paper, whiteness measured, 38.8.
Example 5
Example 5 provides a bio-pulp using peanut shells and reed as raw materials, and a preparation method thereof, comprising the steps of:
inoculating trichoderma strain seed liquid into a 300mLPDA culture medium according to the inoculation amount of 3-5% by mass, performing shake cultivation for 3 days at 30 ℃ and 150rpm, mixing 5g of raw materials according to the proportion of 1: 3 of peanut shells and reed, sterilizing, adding the raw materials into the culture solution, soaking for 8 days, filtering to remove bacterial liquid, pulping soaked reed and peanut shells, washing the pulp, and drying; firstly, 10mL of H with the mass fraction of 30 percent is used2O2+10g NaOH bleached, pressed paper, whiteness measured, 41.3.
Example 6
Example 6 provides a bio-pulp using peanut shells and reed as raw materials, and a preparation method thereof, comprising the steps of:
subjecting Trichoderma toInoculating the strain seed liquid into a 300mLPDA culture medium according to the inoculation amount of 3-5% by mass, carrying out shake cultivation for 3 days at 30 ℃ and 150rpm, mixing 5g of raw materials according to the proportion of 1: 3 of peanut shells to reed, sterilizing, adding the raw materials into the culture liquid, soaking for 8 days, filtering to remove bacterial liquid, pulping the soaked reed and peanut shells, washing the pulp, and drying; firstly, 20mL of H with the mass fraction of 30 percent is used2O2+10g NaOH bleached, pressed paper, whiteness was measured, and the whiteness value was 42.5.
Example 7
inoculating trichoderma strain seed liquid into a 300mLPDA culture medium according to the inoculation amount of 3-5% by mass, performing shake cultivation for 3 days at 30 ℃ and 150rpm, mixing 5g of raw materials according to the proportion of 1: 3 of peanut shells and reed, sterilizing, adding the raw materials into the culture solution, soaking for 8 days, filtering to remove bacterial liquid, pulping soaked reed and peanut shells, washing the pulp, and drying; firstly, 10mL of H with the mass fraction of 30 percent is used2O2+5g NaOH bleached, pressed paper, whiteness measured, 38.8. Then 70mL of mixed enzyme liquid of laccase and xylanase with the ratio of 5:2 is used for bleaching assistance, and the mixture is stirred for 1.5 to 2 hours in a constant temperature water bath kettle at the constant temperature of 40 ℃. Cleaning with clear water for 2-3 times, filtering the paper pulp by suction, flattening the paper pulp when the paper pulp is semi-dry, pressing paper and drying, and measuring the whiteness of the paper by a whiteness meter after drying, wherein the whiteness is 50.1.
The method for preparing the biological paper pulp by using the peanut shells and the reeds as the raw materials has the advantages that the used raw materials of the peanut shells and the reeds are short in growth cycle and rich in yield, and the method is used for pulping and papermaking, can reduce cost, saves forest resources and is beneficial to environmental protection. Meanwhile, the values of the peanut shells and the reeds are improved. The raw material is soaked by the trichoderma to degrade lignin in the raw material, the generation of the cooking black liquor is reduced, and the laccase and the xylanase are used for bleaching the paper pulp in an assistant manner, so that the using amount of a chemical bleaching agent is reduced, and the whiteness of the paper is increased.
In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples and features of different embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction.
Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art within the scope of the present invention.
Claims (7)
1. A preparation method of biological paper pulp taking peanut shells and reeds as raw materials is characterized by comprising the following steps:
s101, inoculating 3-5% of trichoderma in 200-300 mL of potato glucose liquid culture medium according to the inoculation amount, performing shake culture for 2-3 days, and inoculating 3-5% of the inoculation amount of the cultured seed liquid in 0.8-1.2L of potato glucose liquid culture medium for culture for 2-4 days to reach a logarithmic growth phase;
s102: cutting peanut shell into 3mm2～5mm2The small pieces and the reeds are cut into small sections of 1-2 cm, and then the small sections and the reeds are mixed according to the mass ratio of 1: 1-4 and then are sterilized;
s103: adding the strains cultured in the step S101 into the product obtained in the step S102, soaking for 8-10 days, filtering out bacterial liquid, pulping the soaked reed and peanut shells, washing the pulp, and drying to obtain dry paper pulp;
s104: 10 mL-20 mL of H with the mass fraction of 30 percent2O2And 5g to 10gMixing NaOH to obtain mixed liquor, weighing 5-10 g of dry paper pulp, bleaching the dry paper pulp by using the mixed liquor, and then bleaching the dry paper pulp by using a mixed liquor with a mass ratio of 1: (1-9) bleaching the mixed enzyme solution of the laccase and the xylanase, performing suction filtration, flattening filter residues, and drying to obtain biological paper pulp taking peanut shells and reeds as raw materials;
in step S104, after bleaching, the fabric is washed with clean water 2 to 3 times.
2. The method for preparing biological pulp from peanut shells and reeds as claimed in claim 1, wherein in step S102, the sterilization treatment is performed by uv sterilization for 25-35 min.
3. The method for preparing biological pulp from peanut shells and reeds as claimed in claim 1, wherein in step S101, the temperature of shaking culture is 28-32 ℃ and the rotation speed is 140-160 rpm.
4. The method for preparing biological pulp by using peanut shells and reeds as raw materials according to claim 1, wherein in the step S104, the drying temperature is 60-80 ℃, and the drying time is 1-2 h.
5. The method of claim 1, wherein the bleaching in step S104 is carried out at a temperature of 40 ℃ to 50 ℃ using a mixed enzyme solution of laccase and xylanase, and the mixture is stirred for 1.5h to 2 h.
6. A biopaper produced by the method of any of claims 1-5.
7. Use of a biopaper as claimed in any of claims 1 to 6 for the production of paper.
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| CN1779068A (en) * | 2004-11-23 | 2006-05-31 | 中国农业科学院麻类研究所 | High-effective and low-consumption cleaning biological pulp-making process from wheatgrass |
| CN100372996C (en) * | 2005-12-20 | 2008-03-05 | 新疆博湖苇业股份有限公司 | Improved paper pulp bleaching process |
| CN102174399B (en) * | 2010-12-31 | 2013-08-14 | 广西赛尔纸业有限公司 | Method for preparing herbal biological fiber and biological paper pulp |
| CN104088185B (en) * | 2014-06-11 | 2016-06-22 | 华南理工大学 | A kind of biomass-based enzyme pretreatment method for bleaching of paper pulp |
| CN104499336B (en) * | 2014-11-30 | 2018-04-13 | 宁夏天绿健知识产权运营有限公司 | A kind of paper grade (stock) bleaching complex enzyme and preparation method thereof |
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