CN109811087A - Sorghum InDel molecular labeling and its application - Google Patents
Sorghum InDel molecular labeling and its application Download PDFInfo
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Abstract
The invention discloses a kind of sorghum InDel molecular labelings.Including 87 sites, corresponding forward and reverse primer is as shown in sequence table SEQ ID NO:1-SEQ ID NO:174.The present invention is using the genomic DNA of sorghum as research object, it carries out resurveying sequence using Illumina platform, quality control is carried out to sequencing initial data raw data using Trimmomatic software, reject low quality data, the high quality clean data of acquisition and sequencing kind BTx623 are subjected to sequence alignment with reference to genome using BWA software, therefrom detect the site InDel.InDel label disclosed by the invention, can lay the foundation for building, analysis of genetic diversity, cenospecies/Purity, the positioning of sorghum Main Agronomic Characters related gene and the molecular marker assisted selection breeding etc. of sorghum genetic linkage maps.
Description
Technical field
The invention belongs to molecular marking technique fields, and in particular to a kind of sorghum InDel molecular labeling and its application.
Background technique
Sorghum (Sorghum bicolor (L.) Moench) is that the fifth-largest gramineous crop, yield are only second in the world
Corn, wheat, rice and barley are multiduty a kind of C4 plants such as collection grain, feeding, wine brewing, sugar material, biomass energy,
Its photosynthetic efficiency is high, biomass is big, has the multiple resistance such as drought resisting, waterlogging-resistant, saline-alkali tolerant, barren-resistant, plantation is in arid extensively
With semiarid zone.It is closer with corn, cane kind gramineous crop affiliation because its genetic diversity is abundant, and genome
Smaller (about 750M), it is considered to be the another important model plant of gramineous crop genome research carries out heredity in a deep going way to it
Learning research has important theory significance and application value.
Molecular labeling is a kind of genetic marker shape to grow up after morphological markers, cell marking and biochemical marker
Formula, it by protein, nucleic acid molecules mutation based on, detection biological heredity structure make a variation with it, can make up and overcome
Many defects and problem in Morphological Identification and isodynamic enzyme, protein electrophoresis identification, thus it is widely used in crop hybrid kind
Identification, linkage map building, analysis of genetic diversity, comparative genomics, functional gene/QTL positioning, plant origin, classification
With Study on Evolution, marker assisted selection select etc. research.Common molecular labeling in plant genetic research, as restriction fragment is long
Spend polymorphism (RFLP), amplified fragment length polymorphism (AFLP), DNArandom amplified polymorphic DNA (RAPD) and simple repeated sequence
(SSR) label etc..RFLP needs radioisotopic participation, and technology is more complex；RAPD experimental repeatability is poor, as a result reliably
Property it is lower, these disadvantages limit label plant genetic research in application.Compared with the molecular labeling of these early stages, it is based on
Simple repeated sequence (SSR) label of PCR is relatively simple, cheap, repeated preferably, but some amplified bands are unclear
And technical defect causes SSR marker on genotyping and data analysis such as pseudoallele, amorph
It is easy to produce mistake.In recent years, the fast development of genomic sequencing technique, so that high throughput sequencing technologies are that SNP and InDel is marked
The exploitation of note provides possibility.InDel and SNP marker be high-throughput as the third generation, low cost novel molecular labeling type,
It can be obtained by the method for bioinformatics, have the characteristics that economical and practical, specific height, stability are good, be molecular labeling
Assistant breeding provides a kind of selectable method.But SNP is limited to genotyping technology, in small-sized and medium-sized detection
Special equipment is needed, it is at high cost and complicated for operation.And InDel molecular labeling is the sequence based on insertion/deletion site two sides
Specific primer is designed, a kind of molecular labeling of sequence-length polymorphism is embodied by PCR amplification.Compared with SSR marker, InDel
Distribution density is much higher than SSRs.Compared with SNP marker, codominance is presented in InDel label, carries out parting using electrophoresis platform, fastly
It is prompt economical, do not need complicated laboratory apparatus, strong operability.Conventional molecular marker development is generally based on unique sequence, and
InDel marker development is then based on sequence difference, in the process of development variation stablize, accuracy it is high, avoid due to specificity and
The problems such as subsequent analysis caused by complexity obscures.InDel label as high-throughput molecular labeling, have in the plant genome compared with
High distribution frequency has many advantages, such as that genetic stability is high, distribution is wide, polymorphism is strong, versatile, at present in rice, jade
It is used widely in the chief crops such as rice, cotton, cucumber, Chinese cabbage.
With increasingly mature and perfect, theoretical research and practical application of the molecular labeling on sorghum of molecular marking technique
Considerable progress is obtained.Wherein sorghum molecular labeling has obtained extensive concern because its advantage is numerous, past more than ten
Nian Li, the development and application about sorghum label constantly have article report.However molecular marking technique is in China sorghum is studied
Application lag far behind rice, seeding corn and other crops.China is in the then phase of the research in relation to sorghum molecular markers development use aspects
To weakness, exploitation and application in relation to sorghum InDel label are at present still without relevant report.
Summary of the invention
The purpose of the present invention is to provide a kind of sorghum InDel molecular labeling and its applications.
A kind of sorghum InDel molecular labeling includes the corresponding forward and reverse primer in following 87 sites:
The site InDel01: forward primer Chr01-NP2-F and reverse primer Chr01-NP2-R is respectively such as sequence table SEQ
Shown in ID NO:1 and SEQ ID NO:2；
The site InDel02: forward primer Chr01-NP3-F and reverse primer Chr01-NP3-R is respectively such as sequence table SEQ
Shown in ID NO:3 and SEQ ID NO:4；
The site InDel03: forward primer Chr01-NP4-F and reverse primer Chr01-NP4-R is respectively such as sequence table SEQ
Shown in ID NO:5 and SEQ ID NO:6；
The site InDel04: forward primer Chr01-NP6-F and reverse primer Chr01-NP6-R is respectively such as sequence table SEQ
Shown in ID NO:7 and SEQ ID NO:8；
The site InDel05: forward primer Chr02-NP2-F and reverse primer Chr02-NP2-R is respectively such as sequence table SEQ
Shown in ID NO:9 and SEQ ID NO:10；
The site InDel06: forward primer Chr02-NP4-F and reverse primer Chr02-NP4-R is respectively such as sequence table SEQ
Shown in ID NO:11 and SEQ ID NO:12；
The site InDel07: forward primer Chr02-NP7-F and reverse primer Chr02-NP7-R is respectively such as sequence table SEQ
Shown in ID NO:13 and SEQ ID NO:14；
The site InDel08: forward primer Chr02-NP10-F and reverse primer Chr02-NP10-R is respectively such as sequence table SEQ
Shown in ID NO:15 and SEQ ID NO:16；
The site InDel09: forward primer Chr02-NP11-F and reverse primer Chr02-NP11-R is respectively such as sequence table SEQ
Shown in ID NO:17 and SEQ ID NO:18；
The site InDel10: forward primer Chr02-NP12-F and reverse primer Chr02-NP12-R is respectively such as sequence table SEQ
Shown in ID NO:19 and SEQ ID NO:20；
The site InDel11: forward primer Chr02-NP14-F and reverse primer Chr02-NP14-R is respectively such as sequence table SEQ
Shown in ID NO:21 and SEQ ID NO:22；
The site InDel12: forward primer Chr02-MP1-F and reverse primer Chr02-MP1-R is respectively such as sequence table SEQ
Shown in ID NO:23 and SEQ ID NO:24；
The site InDel13: forward primer Chr02-MP2-F and reverse primer Chr02-MP2-R is respectively such as sequence table SEQ
Shown in ID NO:25 and SEQ ID NO:26；
The site InDel14: forward primer Chr02-MP4-F and reverse primer Chr02-MP4-R is respectively such as sequence table SEQ
Shown in ID NO:27 and SEQ ID NO:28；
The site InDel15: forward primer Chr02-MP12-F and reverse primer Chr02-MP12-R is respectively such as sequence table SEQ
Shown in ID NO:29 and SEQ ID NO:30；
The site InDel16: forward primer Chr02-MP13-F and reverse primer Chr02-MP13-R is respectively such as sequence table SEQ
Shown in ID NO:31 and SEQ ID NO:32；
The site InDel17: forward primer Chr02-MP14-F and reverse primer Chr02-MP14-R is respectively such as sequence table SEQ
Shown in ID NO:33 and SEQ ID NO:34；
The site InDel18: forward primer Chr02-MP16-F and reverse primer Chr02-MP16-R is respectively such as sequence table SEQ
Shown in ID NO:35 and SEQ ID NO:36；
The site InDel19: forward primer Chr03-MP3-F and reverse primer Chr03-MP3-R is respectively such as sequence table SEQ
Shown in ID NO:37 and SEQ ID NO:38；
The site InDel20: forward primer Chr03-MP5-F and reverse primer Chr03-MP5-R is respectively such as sequence table SEQ
Shown in ID NO:39 and SEQ ID NO:40；
The site InDel21: forward primer Chr03-MP10-F and reverse primer Chr03-MP10-R is respectively such as sequence table SEQ
Shown in ID NO:41 and SEQ ID NO:42；
The site InDel22: forward primer Chr03-MP12-F and reverse primer Chr03-MP12-R is respectively such as sequence table SEQ
Shown in ID NO:43 and SEQ ID NO:44；
The site InDel23: forward primer Chr03-MP14-F and reverse primer Chr03-MP14-R is respectively such as sequence table SEQ
Shown in ID NO:45 and SEQ ID NO:46；
The site InDel24: forward primer Chr03-NP3-F and reverse primer Chr03-NP3-R is respectively such as sequence table SEQ
Shown in ID NO:47 and SEQ ID NO:48；
The site InDel25: forward primer Chr03-NP4-F and reverse primer Chr03-NP4-R is respectively such as sequence table SEQ
Shown in ID NO:49 and SEQ ID NO:50；
The site InDel26: forward primer Chr03-NP7-F and reverse primer Chr03-NP7-R is respectively such as sequence table SEQ
Shown in ID NO:51 and SEQ ID NO:52；
The site InDel27: forward primer Chr03-NP9-F and reverse primer Chr03-NP9-R is respectively such as sequence table SEQ
Shown in ID NO:53 and SEQ ID NO:54；
The site InDel28: forward primer Chr04-MP1-F and reverse primer Chr04-MP1-R is respectively such as sequence table SEQ
Shown in ID NO:55 and SEQ ID NO:56；
The site InDel29: forward primer Chr04-MP2-F and reverse primer Chr04-MP2-R is respectively such as sequence table SEQ
Shown in ID NO:57 and SEQ ID NO:58；
The site InDel30: forward primer Chr04-MP3-F and reverse primer Chr04-MP3-R is respectively such as sequence table SEQ
Shown in ID NO:59 and SEQ ID NO:60；
The site InDel31: forward primer Chr04-MP13-F and reverse primer Chr04-MP13-R is respectively such as sequence table SEQ
Shown in ID NO:61 and SEQ ID NO:62；
The site InDel32: forward primer Chr04-NP1-F and reverse primer Chr04-NP1-R is respectively such as sequence table SEQ
Shown in ID NO:63 and SEQ ID NO:64；
The site InDel33: forward primer Chr04-NP2-F and reverse primer Chr04-NP2-R is respectively such as sequence table SEQ
Shown in ID NO:65 and SEQ ID NO:66；
The site InDel34: forward primer Chr04-NP6-F and reverse primer Chr04-NP6-R is respectively such as sequence table SEQ
Shown in ID NO:67 and SEQ ID NO:68；
The site InDel35: forward primer Chr04-NP7-F and reverse primer Chr04-NP7-R is respectively such as sequence table SEQ
Shown in ID NO:69 and SEQ ID NO:70；
The site InDel36: forward primer Chr04-NP8-F and reverse primer Chr04-NP8-R is respectively such as sequence table SEQ
Shown in ID NO:71 and SEQ ID NO:72；
The site InDel37: forward primer Chr04-NP11-F and reverse primer Chr04-NP11-R is respectively such as sequence table SEQ
Shown in ID NO:73 and SEQ ID NO:74；
The site InDel38: forward primer Chr05-MP9-F and reverse primer Chr05-MP9-R is respectively such as sequence table SEQ
Shown in ID NO:75 and SEQ ID NO:76；
The site InDel39: forward primer Chr05-MP13-F and reverse primer Chr05-MP13-R is respectively such as sequence table SEQ
Shown in ID NO:77 and SEQ ID NO:78；
The site InDel40: forward primer Chr05-MP14-F and reverse primer Chr05-MP14-R is respectively such as sequence table SEQ
Shown in ID NO:79 and SEQ ID NO:80；
The site InDel41: forward primer Chr05-NP1-F and reverse primer Chr05-NP1-R is respectively such as sequence table SEQ
Shown in ID NO:81 and SEQ ID NO:82；
The site InDel42: forward primer Chr05-NP2-F and reverse primer Chr05-NP2-R is respectively such as sequence table SEQ
Shown in ID NO:83 and SEQ ID NO:84；
The site InDel43: forward primer Chr05-NP5-F and reverse primer Chr05-NP5-R is respectively such as sequence table SEQ
Shown in ID NO:85 and SEQ ID NO:86；
The site InDel44: forward primer Chr05-NP6-F and reverse primer Chr05-NP6-R is respectively such as sequence table SEQ
Shown in ID NO:87 and SEQ ID NO:88；
The site InDel45: forward primer Chr05-NP8-F and reverse primer Chr05-NP8-R is respectively such as sequence table SEQ
Shown in ID NO:89 and SEQ ID NO:90；
The site InDel46: forward primer Chr05-NP9-F and reverse primer Chr05-NP9-R is respectively such as sequence table SEQ
Shown in ID NO:91 and SEQ ID NO:92；
The site InDel47: forward primer Chr05-NP10-F and reverse primer Chr05-NP10-R is respectively such as sequence table SEQ
Shown in ID NO:93 and SEQ ID NO:94；
The site InDel48: forward primer Chr06-MP1-F and reverse primer Chr06-MP1-R is respectively such as sequence table SEQ
Shown in ID NO:95 and SEQ ID NO:96；
The site InDel49: forward primer Chr06-MP8-F and reverse primer Chr06-MP8-R is respectively such as sequence table SEQ
Shown in ID NO:97 and SEQ ID NO:98；
The site InDel50: forward primer Chr06-MP12-F and reverse primer Chr06-MP12-R is respectively such as sequence table SEQ
Shown in ID NO:99 and SEQ ID NO:100；
The site InDel51: forward primer Chr06-NP2-F and reverse primer Chr06-NP2-R is respectively such as sequence table SEQ
Shown in ID NO:101 and SEQ ID NO:102；
The site InDel52: forward primer Chr06-NP7-F and reverse primer Chr06-NP7-R is respectively such as sequence table SEQ
Shown in ID NO:103 and SEQ ID NO:104；
The site InDel53: forward primer Chr07-NP1-F and reverse primer Chr07-NP1-R is respectively such as sequence table SEQ
Shown in ID NO:105 and SEQ ID NO:106；
The site InDel54: forward primer Chr07-NP3-F and reverse primer Chr07-NP3-R is respectively such as sequence table SEQ
Shown in ID NO:107 and SEQ ID NO:108；
The site InDel55: forward primer Chr07-NP6-F and reverse primer Chr07-NP6-R is respectively such as sequence table SEQ
Shown in ID NO:109 and SEQ ID NO:110；
The site InDel56: forward primer Chr07-MP8-F and reverse primer Chr07-MP8-R is respectively such as sequence table SEQ
Shown in ID NO:111 and SEQ ID NO:112；
The site InDel57: forward primer Chr08-MP1-F and reverse primer Chr08-MP1-R is respectively such as sequence table SEQ
Shown in ID NO:113 and SEQ ID NO:114；
The site InDel58: forward primer Chr08-MP12-F and reverse primer Chr08-MP12-R is respectively such as sequence table SEQ
Shown in ID NO:115 and SEQ ID NO:116；
The site InDel59: forward primer Chr08-AP2-F and reverse primer Chr08-AP2-R is respectively such as sequence table SEQ
Shown in ID NO:117 and SEQ ID NO:118；
The site InDel60: forward primer Chr08-AP4-F and reverse primer Chr08-AP4-R is respectively such as sequence table SEQ
Shown in ID NO:119 and SEQ ID NO:120；
The site InDel61: forward primer Chr08-AP5-F and reverse primer Chr08-AP5-R is respectively such as sequence table SEQ
Shown in ID NO:121 and SEQ ID NO:122；
The site InDel62: forward primer Chr08-NP3-F and reverse primer Chr08-NP3-R is respectively such as sequence table SEQ
Shown in ID NO:123 and SEQ ID NO:124；
The site InDel63: forward primer Chr09-MP1-F and reverse primer Chr09-MP1-R is respectively such as sequence table SEQ
Shown in ID NO:125 and SEQ ID NO:126；
The site InDel64: forward primer Chr09-YM1-F and reverse primer Chr09-YM1-R is respectively such as sequence table SEQ
Shown in ID NO:127 and SEQ ID NO:128；
The site InDel65: forward primer Chr09-YM2-F and reverse primer Chr09-YM2-R is respectively such as sequence table SEQ
Shown in ID NO:129 and SEQ ID NO:130；
The site nDel66: forward primer Chr09-YM5-F and reverse primer Chr09-YM5-R is respectively such as sequence table SEQ ID
Shown in NO:131 and SEQ ID NO:132；
The site nDel67: forward primer Chr09-YM10-F and reverse primer Chr09-YM10-R is respectively such as sequence table SEQ
Shown in ID NO:133 and SEQ ID NO:134；
The site nDel68: forward primer Chr09-YM11-F and reverse primer Chr09-YM11-R is respectively such as sequence table SEQ
Shown in ID NO:135 and SEQ ID NO:136；
The site InDel69: forward primer Chr09-MP4-F and reverse primer Chr09-MP4-R is respectively such as sequence table SEQ
Shown in ID NO:137 and SEQ ID NO:138；
The site InDel70: forward primer Chr09-MP5-F and reverse primer Chr09-MP5-R is respectively such as sequence table SEQ
Shown in ID NO:139 and SEQ ID NO:140；
The site InDel71: forward primer Chr09-MP9-F and reverse primer Chr09-MP9-R is respectively such as sequence table SEQ
Shown in ID NO:141 and SEQ ID NO:142；
The site InDel72: forward primer Chr09-MP11-F and reverse primer Chr09-MP11-R is respectively such as sequence table SEQ
Shown in ID NO:143 and SEQ ID NO:144；
The site InDel73: forward primer Chr09-NP1-F and reverse primer Chr09-NP1-R is respectively such as sequence table SEQ
Shown in ID NO:145 and SEQ ID NO:146；
The site InDel74: forward primer Chr09-NP2-F and reverse primer Chr09-NP2-R is respectively such as sequence table SEQ
Shown in ID NO:147 and SEQ ID NO:148；
The site InDel75: forward primer Chr09-NP4-F and reverse primer Chr09-NP4-R is respectively such as sequence table SEQ
Shown in ID NO:149 and SEQ ID NO:150；
The site InDel76: forward primer Chr09-NP5-F and reverse primer Chr09-NP5-R is respectively such as sequence table SEQ
Shown in ID NO:151 and SEQ ID NO:152；
The site InDel77: forward primer Chr09-NP9-F and reverse primer Chr09-NP9-R is respectively such as sequence table SEQ
Shown in ID NO:153 and SEQ ID NO:154；
The site InDel78: forward primer Chr10-MP2-F and reverse primer Chr10-MP2-R is respectively such as sequence table SEQ
Shown in ID NO:155 and SEQ ID NO:156；
The site InDel79: forward primer Chr10-MP5-F and reverse primer Chr10-MP5-R is respectively such as sequence table SEQ
Shown in ID NO:157 and SEQ ID NO:158；
The site InDel80: forward primer Chr10-MP7-F and reverse primer Chr10-MP7-R is respectively such as sequence table SEQ
Shown in ID NO:159 and SEQ ID NO:160；
The site InDel81: forward primer Chr10-MP8-F and reverse primer Chr10-MP8-R is respectively such as sequence table SEQ
Shown in ID NO:161 and SEQ ID NO:162；
The site InDel82: forward primer Chr10-MP11-F and reverse primer Chr10-MP11-R is respectively such as sequence table SEQ
Shown in ID NO:163 and SEQ ID NO:164；
The site InDel83: forward primer Chr10-NP4-F and reverse primer Chr10-NP4-R is respectively such as sequence table SEQ
Shown in ID NO:165 and SEQ ID NO:166；
The site InDel84: forward primer Chr10-NP6-F and reverse primer Chr10-NP6-R is respectively such as sequence table SEQ
Shown in ID NO:167 and SEQ ID NO:168；
The site InDel85: forward primer Chr10-NP7-F and reverse primer Chr10-NP7-R is respectively such as sequence table SEQ
Shown in ID NO:169 and SEQ ID NO:170；
The site InDel86: forward primer Chr10-NP9-F and reverse primer Chr10-NP9-R is respectively such as sequence table SEQ
Shown in ID NO:171 and SEQ ID NO:172；
The site InDel87: forward primer Chr10-NP11-F and reverse primer Chr10-NP11-R is respectively such as sequence table SEQ
Shown in ID NO:173 and SEQ ID NO:174.
Application of the above-mentioned sorghum InDel molecular labeling in the building of sorghum genetic linkage maps.
InDel label is widely used for the building of crop linkage map as a kind of important genetic marker.The present invention
Using the physical map (Fig. 2) of Mapchat Software on Drawing this 87 InDel molecular labelings on Chromosome in Sorghum, lost for sorghum
The building for passing linkage map lays the foundation.
Application of the above-mentioned sorghum InDel molecular labeling in Sorghum Germplasm genetic diversity Journal of Sex Research.
InDel molecular marking technique is marked as a kind of DNA fingerprint of high throughput, can preferably be disclosed in genome
Polymorphism, therefore there is superiority more outstanding in terms of germplasm identification, in the crops such as corn, rice, Chinese cabbage
It is used widely.The polymorphism InDel primer of exploitation of the present invention, therefrom randomly selects two pairs of polymorphism primers
(InDel20, InDel38) identifies 11 parts of sorghum varieties in conjunction with sorghum seedling genome DNA rapid extraction method,
PCR amplification is the results show that different cultivars shows varietY specificity (Fig. 3) under the detection of different primers.The result shows that
InDel technology can be used for extensive DNA fingerprint analysis as a kind of quick, accurate, high efficiency method.What the present invention was developed
InDel label can play an important role in Sorghum Germplasm identification.
Application of the above-mentioned sorghum InDel molecular labeling in sorghum hybrid/Purity.
Randomly selecting 3 through PCR verifying there is the InDel of polymorphism to mark, and quickly mention in conjunction with Sorghum Leaves genomic DNA
Method is taken, cenospecies validity and Purity assessment have been carried out to 17 filial generations for preparing 2 hybrid Populations of sorghum, according to
The electrophoresis result of pcr amplification product compares and analyzes father, female parent and hybrid generation, if to amplify band big for hybrid generation
The complete complementary type (biobelt) of small completely the same with Parent/performance Parent band is true hybrid, and expands and only show
The offspring of the single band or other clip sizes of similar male parent or female parent is pseudostationary (Fig. 4).Show that Indel label can quilt
For quick, accurate and economically identification sorghum hybrid authenticity and purity.
Application of the above-mentioned sorghum InDel molecular labeling in terms of the positioning of sorghum Main Agronomic Characters related gene.
Beneficial effects of the present invention:
The present invention is based on the exploitations of high throughput genome sequencing data technique based on PCR, easy to operate, application value is big
InDel label, can be the buildings of sorghum genetic linkage maps, analysis of genetic diversity, sorghum hybrid/Purity, height
Positioning and molecular marker assisted selection breeding of fine strain of millet Main Agronomic Characters related gene etc. lay the foundation.
The InDel label that the present invention develops is using high throughput Illumina sequencing technologies to sorghum conventional variety
Jiutian1 genome carries out resurveying sequence, by with sequencing kind BTx623 with reference to genome sequence carry out it is homologous compare, obtain
Insertion/deletion site abundant.According to sequence difference (> 10bp) of these sites InDel between two gene pools and in sorghum
Distribution situation on 10 chromosomes, the site InDel that selection variation is stablized in the process of development, accuracy is high carry out primer and set
Meter, to avoid the problem fuzzy due to subsequent analysis caused by specificity and complexity.The present invention devises about 100 pairs and draws
Object, verifying 87 pairs of InDel primers obtained through PCR has the characteristics that polymorphism is high, distribution is wide, stability is high.
InDel primer development based on PCR amplification of the present invention, is detected using 4% agarose gel electrophoresis platform, is expanded
It is clear to increase band, polymorphism height, high resolution, and simple and easy to do, strong operability, it is economical and practical.
Detailed description of the invention
Fig. 1 is that 87 InDel of sorghum mark PCR product agarose gel electrophoresis testing result；
In figure, M--DNA Marker, for the Genstar Direct-Load of Kang Run Biotechnology Co., Ltd productionTM
Star Marker (D5000), by 5000bp, 3000bp, 2000bp, 1500bp, 1000bp, 750bp, 500bp, 250bp,
100bp band composition；1. sorghum BTx623；2. sorghum Jiutian1.
Sorghum physical map constructed by 87 InDel molecular labelings of the Fig. 2 based on exploitation.
Fig. 3 carries out the electrophorogram of sorghum variety analysis of genetic diversity using sorghum InDel molecular labeling；
In figure: M-DNA Marker (D2000)；1-11 is different sorghum varieties: the white snake eye of 1-, the river 2-Wheatland, 3-
North glutinous miscellaneous, the beautiful Europe 4-, 5-T32,6-SEM-E32,7-SEM-42,8-ZIH08894,9-E-Tian, 10-Jiutian1,11-
BTx623。
Fig. 4 marks the electrophorogram for carrying out sorghum hybrid identification using InDel；
In figure: M-DNA Marker (D2000) II；1:JiutianYM1；2:BTx623；3-7:JiutianYM1 with
BTx623 hybrid generation；8:JiutianYM2；9:BTx623；10-13:JiutianYM2 and BTx623 hybrid generation；12:
JiutianYM1；13:BTx623；14-17:JiutianYM1 and BTx623 hybrid generation；18:JiutianYM2；19:
BTx623；20-23:JiutianYM2 and BTx623 hybrid generation.
Specific embodiment
The present invention will be further described combined with specific embodiments below.
1 sorghum extracting genome DNA of embodiment
1g young leaflet tablet is taken in sorghum variety Jiutian1 tri-leaf period, after liquid nitrogen grinding is broken, is transferred to 2.0mL
In Eppendorf centrifuge tube, 700 μ L CTAB lysate [2%CTAB, 2M NaCl, 20mM EDTA, 100mM Tris- are added
HCl (pH=8.0), 0.2% beta -mercaptoethanol (with with matching)], 65 DEG C of water-bath 0.5h~1h are placed in after mixing well, it
Afterwards take out be cooled to room temperature, isometric phenol: chloroform is added: gently shaken after isoamyl alcohol (24:23:1) mixture 5min~
Milk is centrifuged 8min~10min with 12000r/min revolving speed room temperature by 10min, and supernatant is taken to be transferred to new 1.5ml centrifugation
Guan Zhong, isometric chloroform is added: isoamyl alcohol (24:1) extracts 1 time~2 times, extracts supernatant and the different of 2 times of volume pre-coolings is added
Mixture after standing is centrifuged 10min with 12000r/min revolving speed, supernatant is abandoned, with 70% second by propyl alcohol, 4 DEG C of standing 3h or more
Alcohol washs DNA and precipitates 2-3 times, and 12000r/min revolving speed is centrifuged 3min each time after washing.Supernatant is abandoned, at room temperature by DNA
It is dry, appropriate 1xTE solution or ddH is added2In O, saved backup to 4 DEG C after completely dissolution.Utilize ultraviolet/visible light photometer
Carry out DNA purity analysis and quantitative detection.
The design of 2 sorghum InDel labeled primer of embodiment
DNA library is constructed using TruSeq DNA PCR-Free LT Library Preparation Kit, is utilized
Illumina HiSeq XTMTen microarray dataset carries out sorghum variety Jiutian1 to resurvey sequence；Utilize Trimmomatic software
Quality Control is carried out to sequence, is carried out Clean data and sorghum sequencing kind BTx623 with reference to genome sequence using BWA software
Compare (Https: //genome.jgi.doe.gov/portal/pages/dynamicOrganismDownload. jsf? Organism=Sbicolor).Sequence difference >=10bp InDel in screening target area is compared by extensive homologous sequence
Site utilizes 5.0 software design InDel labeled primer of Primer Premier.The condition of design of primers: pcr amplification product is long
Degree is 170-300bp；Denaturation temperature (Tm) is best with 56 DEG C between 50-62 DEG C；G/C content between 40-60%, with
50% is best；Primer length is best with 20bp in 18-25bp.Therefrom pick 100 of covering 10 chromosome of sorghum
Label is used for polymorphism analysis.100 pairs of primers are devised altogether, are synthesized by Sangon Biotech (Shanghai) Co., Ltd., are passed through
There is the InDel primer of polymorphism to have 87 pairs for PCR verifying, primer sequence such as sequence table SEQ ID NO:1-SEQ ID NO:158
It is shown.
The PCR amplification of 3 InDel primer of embodiment
The PCR amplification that the InDel primer of synthesis first passes through (sorghum Jiutian1 and BTx623) between 2 gene pools carries out more
State property screening.Pcr amplification reaction system is 20 μ L, wherein containing 1.0 μ L genomic DNAs (200ng/ μ l), 17 1 × Golden of μ L
Star T6Super PCR Mix (Beijing Qing Ke Biotechnology Co., Ltd), 1.0 μ L forward primers (10 μM), 1.0 μ L are reversed
Primer (10 μM).PCR amplification program are as follows: 98 DEG C of initial denaturation 2min；98 DEG C of denaturation 10sec, Tm annealing (50~62 DEG C) 10sec,
72 DEG C of extension 10sec, 35 circulations；72 DEG C of extension 5min are saved in 4 DEG C.At Hangzhou BIOER Technology Co., Ltd (BIOER)
PCR amplification is carried out on LifeECO gene-amplificative instrament (TC-96/G/H (b) C).
The detection of 4 agarose gel electrophoresis of embodiment
Ago-Gel preparation: weighing 4g agarose (Agarose, Invitrogen), and it is slow that 50 × TAE of 2mL electrophoresis is added
Fliud flushing (12.2g Tris, 2.85mL glacial acetic acid, 10mL 0.25mol/L EDTA (PH 8.0) add water to 50mL) and 98mL steam
Distilled water, heating for dissolving are made into 4.0% Ago-Gel 100mL.After slightly cold, 0.5 μ g/mL EB solution is added and mixes；It installs
Electrophoresis tank and sample comb, encapsulating pour into 1 × TAE electrophoretic buffer after the cooling condensation of glue in electrophoresis tank.5 μ L PCR are taken to expand
Increase production object and 1 μ L 6 × DNA Loading Buffer is added, is loaded after mixing with pipettor.Use electrophoresis apparatus (DYY-6C type, north
61 Biotechnology Co., Ltd of capital) for connection electrode in 60V constant-pressure conditions progress electrophoresis, electrophoresis time is 1.0h~1.5h, with
Until the DNA band of amplification is sufficiently spread out.
It is detected by the agarose gel electrophoresis to 100 pairs of InDel primer PCR amplified productions, to InDel primer in sorghum
Polymorphism in kind Jiutian1 and sequencing kind BTx623 gene pool is screened, and is finally screened to 87 pairs of primer amplifications and is imitated
Fruit is good and shows the InDel of polymorphism label (Fig. 1).These InDel label can be applied to sorghum variety identification, genetic map
The researchs such as spectrum building, functional gene/QTL positioning, marker assisted selection and analysis of genetic diversity.
Sequence table
<110>Biological Technology institute, Chinese Academy of Agricultural Sciences
<120>sorghum InDel molecular labeling and its application
<160> 174
<170> SIPOSequenceListing 1.0
<210> 1
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 1
tggtcgaaca agactgcaac 20
<210> 2
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 2
cactacggcg aaattggaag 20
<210> 3
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 3
atcggcatcc atcagatttt 20
<210> 4
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 4
cctgccaact cagcaatttt 20
<210> 5
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 5
tctttcccag tggaacaacc 20
<210> 6
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 6
caggggactc ttcaaactgc 20
<210> 7
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 7
agatccgagc atttctgctg 20
<210> 8
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 8
agttcgccca ggtaaggtct 20
<210> 9
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 9
tttcatggct ttgagtgtca 20
<210> 10
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 10
acgctaacag agggcagtgt 20
<210> 11
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 11
tggtcaagtt ttggcatgaa 20
<210> 12
<211> 24
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 12
gtcatgttct ttctgattcc ctct 24
<210> 13
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 13
tgcctctatt tttcccatgc 20
<210> 14
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 14
aatgccaagt ttggtccatc 20
<210> 15
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 15
tacacacagt acgcccatgc 20
<210> 16
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 16
ggtttggtgt ctggcaactt 20
<210> 17
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 17
atatctggtt cccccagctt 20
<210> 18
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 18
gctcagctcg tctcgtctct 20
<210> 19
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 19
ttttgcgaca cctactgctg 20
<210> 20
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 20
ggttttctgg ggtgcataga 20
<210> 21
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 21
tgcaatacct gttccccact 20
<210> 22
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 22
caacaatggc tacagcaacg 20
<210> 23
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 23
cctgacccag acacacacac 20
<210> 24
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 24
agcaatcttg tcaggggatg 20
<210> 25
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 25
gctgatggct ctccaaactc 20
<210> 26
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 26
gttggtccct gaatgcttgt 20
<210> 27
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 27
tgacctaagg ggttgtttgg 20
<210> 28
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 28
gcacctgcca catcctattt 20
<210> 29
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 29
caaaggccga tgtatttgct 20
<210> 30
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 30
tgtgccaacc ctgtatgtgt 20
<210> 31
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 31
gaaggcgctc gtgaatttta 20
<210> 32
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 32
cagacacaaa ggctgtctcg 20
<210> 33
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 33
ccgcacaaag tagagcatca 20
<210> 34
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 34
ccgtaagaga agcgagccta 20
<210> 35
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 35
ttttcagtct ggtcgcttga 20
<210> 36
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 36
cacacctcac tcgcttctca 20
<210> 37
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 37
ggcacatgca tgaagcacta 20
<210> 38
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 38
tgattcgaca gcttccagag 20
<210> 39
<211> 22
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 39
cgagaagaca tgacaaaacg ag 22
<210> 40
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 40
ctttggtcgc atgtatggaa 20
<210> 41
<211> 23
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 41
ccatctggat atacacgggt tag 23
<210> 42
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 42
tgcaaaccgg ttctcaaagt 20
<210> 43
<211> 21
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 43
tgcatgcacg tagtagacac a 21
<210> 44
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 44
caccacgata gcaaatcacc 20
<210> 45
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 45
ggtgcagtgg tgtcttcctt 20
<210> 46
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 46
atttacagcg cgtggctatc 20
<210> 47
<211> 22
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 47
ttaaggtctc gtttagttca gg 22
<210> 48
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 48
gccttgttca gttggcaaaa 20
<210> 49
<211> 22
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 49
tcagatgagc aggctctaca gt 22
<210> 50
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 50
gagcattttc ggcatgtgta 20
<210> 51
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 51
acgaaagaaa tgcaccgaac 20
<210> 52
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 52
cccagcctag gaaacatcaa 20
<210> 53
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 53
gcgtgactat tgcagctgtt 20
<210> 54
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 54
catgccatcc acttccacta 20
<210> 55
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 55
gcggtcatag gcgttactgt 20
<210> 56
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 56
cgctgtcaca tcgsstcttt 20
<210> 57
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 57
tggataggtt catgctgctg 20
<210> 58
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 58
tttttgccac cttccaattc 20
<210> 59
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 59
ctgccctatg catctgttca 20
<210> 60
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 60
ccaactagtt cagggcagga 20
<210> 61
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 61
tccgtccaac tggaattgat 20
<210> 62
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 62
aatccttcgt ggaaagctga 20
<210> 63
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 63
atcttgcggc acatacatga 20
<210> 64
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 64
cttcaacact cagcccacct 20
<210> 65
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 65
tataaagcac cggtggaggt 20
<210> 66
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 66
acaaatcgtt tcaccgcttc 20
<210> 67
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 67
ggctgttgga ggaattttga 20
<210> 68
<211> 24
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 68
ccttgtactc ttctgagttt tgga 24
<210> 69
<211> 25
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 69
gaccgagaaa gaaaatcata cactc 25
<210> 70
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 70
aggcaaaaag cccaactctt 20
<210> 71
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 71
atgccgtaga tcgtcctcct 20
<210> 72
<211> 18
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 72
caaccggagc tggaaatg 18
<210> 73
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 73
agctcatcaa agcgaaagga 20
<210> 74
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 74
agtctagacg cctcgacgtt 20
<210> 75
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 75
atgggaaggc ctacgatttt 20
<210> 76
<211> 22
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 76
tgggtgatgg taggagaata ga 22
<210> 77
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 77
atttaacctg gctgcctgtc 20
<210> 78
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 78
gaccgcgaca ctaacaaatg 20
<210> 79
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 79
tttgcctagc catttcaggt 20
<210> 80
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 80
cacggtcaac aatcagctct 20
<210> 81
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 81
agcattggct gctaagcatt 20
<210> 82
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 82
tagcagcaac agcaaacagg 20
<210> 83
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 83
agagagaatc ctcggcaaga 20
<210> 84
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 84
tttgtgaggg gttcttgagc 20
<210> 85
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 85
cttcgtggac cacatgacac 20
<210> 86
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 86
gtaccctcgc cagtcaactc 20
<210> 87
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 87
tctcacgggt cgaaagtacc 20
<210> 88
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 88
caatctcgat tgcctcaaca 20
<210> 89
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 89
ttgtgggatg aaaggagcat 20
<210> 90
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 90
gcctgtacca gaaaccgaac 20
<210> 91
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 91
agaatgttgc acgcattgac 20
<210> 92
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 92
gccgaccatt atttgtgacc 20
<210> 93
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 93
gcaattgcaa catcgaacac 20
<210> 94
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 94
tccacatcca tcctcatcaa 20
<210> 95
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 95
cataggattt tgccacgaca 20
<210> 96
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 96
cgtcacagag tccatgcaga 20
<210> 97
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 97
tgcgaaaaga aaaaggatgg 20
<210> 98
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 98
cccaaaatcc actttcctga 20
<210> 99
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 99
tacgctggtg cttacgaaaa 20
<210> 100
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 100
gggagaggaa aggtgaaacc 20
<210> 101
<211> 19
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 101
attgatttgg ggtcgggta 19
<210> 102
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 102
caccgcaagc caaaatagag 20
<210> 103
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 103
agtggctgta cgggtttgac 20
<210> 104
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 104
ttgtgcagca tcaacgtgta 20
<210> 105
<211> 21
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 105
tggatccaag taaacccgat a 21
<210> 106
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 106
acttaaaggc cccactgaaa 20
<210> 107
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 107
taacggtttg ggtcttcgag 20
<210> 108
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 108
gcgatgaaaa acacagcaaa 20
<210> 109
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 109
ttctggtctg gtgaatgcaa 20
<210> 110
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 110
tccatgacga acgagaaatc 20
<210> 111
<211> 19
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 111
gccaagtttg ccaaaatgc 19
<210> 112
<211> 19
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 112
gttaacgcaa cccagcaga 19
<210> 113
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 113
gccgagtgtc tcgttagacc 20
<210> 114
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 114
cacgacggca ccctactaat 20
<210> 115
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 115
gaaccaaaca gcgtcagacc 20
<210> 116
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 116
agcaccaccc ataacgtgtc 20
<210> 117
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 117
tgactgtgtc gctaccttgc 20
<210> 118
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 118
gatggcaaga gtgggatttg 20
<210> 119
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 119
tccaaaccgg tggataattg 20
<210> 120
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 120
aagccttgtt tggatgctgt 20
<210> 121
<211> 18
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 121
caaaggacga gataagca 18
<210> 122
<211> 18
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 122
ttccctgtca catcaaat 18
<210> 123
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 123
tccatgcata tgcctcaaaa 20
<210> 124
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 124
caagttgtgc agttgctggt 20
<210> 125
<211> 22
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 125
gacggagata cttagggcag aa 22
<210> 126
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 126
tctctctcct ccctttccaa 20
<210> 127
<211> 18
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 127
gtcggcgtag ctcatctt 18
<210> 128
<211> 22
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 128
gtagcacttt cgtttgtatt tg 22
<210> 129
<211> 18
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 129
gtcaaggagg aaccaaag 18
<210> 130
<211> 18
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 130
atgcctaagc ctaagtaa 18
<210> 131
<211> 18
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 131
gtcaaggagg aaccaaag 18
<210> 132
<211> 18
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 132
atgcctaagc ctaagtaa 18
<210> 133
<211> 18
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 133
tcggcttgat ttgttctt 18
<210> 134
<211> 18
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 134
ctggctgctg agtaagtg 18
<210> 135
<211> 18
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 135
cgaaatgctt cacgctgt 18
<210> 136
<211> 19
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 136
gaaacctacc caatgtccg 19
<210> 137
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 137
ttccaataaa gctgccgttc 20
<210> 138
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 138
atactagcgg tgcgggttac 20
<210> 139
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 139
ctgaacaagg cctaggtgga 20
<210> 140
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 140
gctcaccact gcaactgcta 20
<210> 141
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 141
atgtcagcga aatggtttcc 20
<210> 142
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 142
caccaagtcc ttgctcatca 20
<210> 143
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 143
atggaatatg agccccaaca 20
<210> 144
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 144
atcaggtcgt ccaccagttc 20
<210> 145
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 145
acgaggatgg caagaggata 20
<210> 146
<211> 19
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 146
gacggtgcat gtgcttttt 19
<210> 147
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 147
gcggatgagg aggaatctct 20
<210> 148
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 148
tttactgcgc ctcgtctctt 20
<210> 149
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 149
agcactagca aatgccacaa 20
<210> 150
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 150
atgacgtttt tcggcttgtc 20
<210> 151
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 151
gctgagcact tgagggttgt 20
<210> 152
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 152
ctggtcatcg gcttagcaat 20
<210> 153
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 153
gcggcttatc acagctccta 20
<210> 154
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 154
tcaccatgca aaacgtccta 20
<210> 155
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 155
agagatcgag aggacggaca 20
<210> 156
<211> 19
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 156
ggaattccat gcccaaatc 19
<210> 157
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 157
gcatttgcat ctgaaagctc 20
<210> 158
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 158
accaaactct gcaggaatgc 20
<210> 159
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 159
aggcgttgcc tctaacactg 20
<210> 160
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 160
gtccggtgag ttcgtttctc 20
<210> 161
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 161
cacatggttc ttttccacga 20
<210> 162
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 162
ggagttggat gaaagccttg 20
<210> 163
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 163
tggtagacgg gtcctagtgc 20
<210> 164
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 164
tacatcgctg ctggagtgtc 20
<210> 165
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 165
acacgggtcg aagatcaaag 20
<210> 166
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 166
ggtgccaact tccttcatca 20
<210> 167
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 167
tttgcaagct gttttggttc 20
<210> 168
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 168
gggtgaaggc tacatcggta 20
<210> 169
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 169
taccgttgtc cgacgctaat 20
<210> 170
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 170
ttgtgtaggc gtcgtgctta 20
<210> 171
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 171
cgaccctgtc gtatgtatgc 20
<210> 172
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 172
ttgtccttct gtggctgttg 20
<210> 173
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 173
acggaatgca aaaagcaaag 20
<210> 174
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 174
ggtgacttca accgggatta 20
Claims (5)
1. a kind of sorghum InDel molecular labeling, which is characterized in that include that the corresponding forward and reverse in following 87 sites draws
Object:
The site InDel01: forward primer Chr01-NP2-F and reverse primer Chr01-NP2-R is respectively such as sequence table SEQ ID
Shown in NO:1 and SEQ ID NO:2；
The site InDel02: forward primer Chr01-NP3-F and reverse primer Chr01-NP3-R is respectively such as sequence table SEQ ID
Shown in NO:3 and SEQ ID NO:4；
The site InDel03: forward primer Chr01-NP4-F and reverse primer Chr01-NP4-R is respectively such as sequence table SEQ ID
Shown in NO:5 and SEQ ID NO:6；
The site InDel04: forward primer Chr01-NP6-F and reverse primer Chr01-NP6-R is respectively such as sequence table SEQ ID
Shown in NO:7 and SEQ ID NO:8；
The site InDel05: forward primer Chr02-NP2-F and reverse primer Chr02-NP2-R is respectively such as sequence table SEQ ID
Shown in NO:9 and SEQ ID NO:10；
The site InDel06: forward primer Chr02-NP4-F and reverse primer Chr02-NP4-R is respectively such as sequence table SEQ ID
Shown in NO:11 and SEQ ID NO:12；
The site InDel07: forward primer Chr02-NP7-F and reverse primer Chr02-NP7-R is respectively such as sequence table SEQ ID
Shown in NO:13 and SEQ ID NO:14；
The site InDel08: forward primer Chr02-NP10-F and reverse primer Chr02-NP10-R is respectively such as sequence table SEQ ID
Shown in NO:15 and SEQ ID NO:16；
The site InDel09: forward primer Chr02-NP11-F and reverse primer Chr02-NP11-R is respectively such as sequence table SEQ ID
Shown in NO:17 and SEQ ID NO:18；
The site InDel10: forward primer Chr02-NP12-F and reverse primer Chr02-NP12-R is respectively such as sequence table SEQ ID
Shown in NO:19 and SEQ ID NO:20；
The site InDel11: forward primer Chr02-NP14-F and reverse primer Chr02-NP14-R is respectively such as sequence table SEQ ID
Shown in NO:21 and SEQ ID NO:22；
The site InDel12: forward primer Chr02-MP1-F and reverse primer Chr02-MP1-R is respectively such as sequence table SEQ ID
Shown in NO:23 and SEQ ID NO:24；
The site InDel13: forward primer Chr02-MP2-F and reverse primer Chr02-MP2-R is respectively such as sequence table SEQ ID
Shown in NO:25 and SEQ ID NO:26；
The site InDel14: forward primer Chr02-MP4-F and reverse primer Chr02-MP4-R is respectively such as sequence table SEQ ID
Shown in NO:27 and SEQ ID NO:28；
The site InDel15: forward primer Chr02-MP12-F and reverse primer Chr02-MP12-R is respectively such as sequence table SEQ ID
Shown in NO:29 and SEQ ID NO:30；
The site InDel16: forward primer Chr02-MP13-F and reverse primer Chr02-MP13-R is respectively such as sequence table SEQ ID
Shown in NO:31 and SEQ ID NO:32；
The site InDel17: forward primer Chr02-MP14-F and reverse primer Chr02-MP14-R is respectively such as sequence table SEQ ID
Shown in NO:33 and SEQ ID NO:34；
The site InDel18: forward primer Chr02-MP16-F and reverse primer Chr02-MP16-R is respectively such as sequence table SEQ ID
Shown in NO:35 and SEQ ID NO:36；
The site InDel19: forward primer Chr03-MP3-F and reverse primer Chr03-MP3-R is respectively such as sequence table SEQ ID
Shown in NO:37 and SEQ ID NO:38；
The site InDel20: forward primer Chr03-MP5-F and reverse primer Chr03-MP5-R is respectively such as sequence table SEQ ID
Shown in NO:39 and SEQ ID NO:40；
The site InDel21: forward primer Chr03-MP10-F and reverse primer Chr03-MP10-R is respectively such as sequence table SEQ ID
Shown in NO:41 and SEQ ID NO:42；
The site InDel22: forward primer Chr03-MP12-F and reverse primer Chr03-MP12-R is respectively such as sequence table SEQ ID
Shown in NO:43 and SEQ ID NO:44；
The site InDel23: forward primer Chr03-MP14-F and reverse primer Chr03-MP14-R is respectively such as sequence table SEQ ID
Shown in NO:45 and SEQ ID NO:46；
The site InDel24: forward primer Chr03-NP3-F and reverse primer Chr03-NP3-R is respectively such as sequence table SEQ ID
Shown in NO:47 and SEQ ID NO:48；
The site InDel25: forward primer Chr03-NP4-F and reverse primer Chr03-NP4-R is respectively such as sequence table SEQ ID
Shown in NO:49 and SEQ ID NO:50；
The site InDel26: forward primer Chr03-NP7-F and reverse primer Chr03-NP7-R is respectively such as sequence table SEQ ID
Shown in NO:51 and SEQ ID NO:52；
The site InDel27: forward primer Chr03-NP9-F and reverse primer Chr03-NP9-R is respectively such as sequence table SEQ ID
Shown in NO:53 and SEQ ID NO:54；
The site InDel28: forward primer Chr04-MP1-F and reverse primer Chr04-MP1-R is respectively such as sequence table SEQ ID
Shown in NO:55 and SEQ ID NO:56；
The site InDel29: forward primer Chr04-MP2-F and reverse primer Chr04-MP2-R is respectively such as sequence table SEQ ID
Shown in NO:57 and SEQ ID NO:58；
The site InDel30: forward primer Chr04-MP3-F and reverse primer Chr04-MP3-R is respectively such as sequence table SEQ ID
Shown in NO:59 and SEQ ID NO:60；
The site InDel31: forward primer Chr04-MP13-F and reverse primer Chr04-MP13-R is respectively such as sequence table SEQ ID
Shown in NO:61 and SEQ ID NO:62；
The site InDel32: forward primer Chr04-NP1-F and reverse primer Chr04-NP1-R is respectively such as sequence table SEQ ID
Shown in NO:63 and SEQ ID NO:64；
The site InDel33: forward primer Chr04-NP2-F and reverse primer Chr04-NP2-R is respectively such as sequence table SEQ ID
Shown in NO:65 and SEQ ID NO:66；
The site InDel34: forward primer Chr04-NP6-F and reverse primer Chr04-NP6-R is respectively such as sequence table SEQ ID
Shown in NO:67 and SEQ ID NO:68；
The site InDel35: forward primer Chr04-NP7-F and reverse primer Chr04-NP7-R is respectively such as sequence table SEQ ID
Shown in NO:69 and SEQ ID NO:70；
The site InDel36: forward primer Chr04-NP8-F and reverse primer Chr04-NP8-R is respectively such as sequence table SEQ ID
Shown in NO:71 and SEQ ID NO:72；
The site InDel37: forward primer Chr04-NP11-F and reverse primer Chr04-NP11-R is respectively such as sequence table SEQ ID
Shown in NO:73 and SEQ ID NO:74；
The site InDel38: forward primer Chr05-MP9-F and reverse primer Chr05-MP9-R is respectively such as sequence table SEQ ID
Shown in NO:75 and SEQ ID NO:76；
The site InDel39: forward primer Chr05-MP13-F and reverse primer Chr05-MP13-R is respectively such as sequence table SEQ ID
Shown in NO:77 and SEQ ID NO:78；
The site InDel40: forward primer Chr05-MP14-F and reverse primer Chr05-MP14-R is respectively such as sequence table SEQ ID
Shown in NO:79 and SEQ ID NO:80；
The site InDel41: forward primer Chr05-NP1-F and reverse primer Chr05-NP1-R is respectively such as sequence table SEQ ID
Shown in NO:81 and SEQ ID NO:82；
The site InDel42: forward primer Chr05-NP2-F and reverse primer Chr05-NP2-R is respectively such as sequence table SEQ ID
Shown in NO:83 and SEQ ID NO:84；
The site InDel43: forward primer Chr05-NP5-F and reverse primer Chr05-NP5-R is respectively such as sequence table SEQ ID
Shown in NO:85 and SEQ ID NO:86；
The site InDel44: forward primer Chr05-NP6-F and reverse primer Chr05-NP6-R is respectively such as sequence table SEQ ID
Shown in NO:87 and SEQ ID NO:88；
The site InDel45: forward primer Chr05-NP8-F and reverse primer Chr05-NP8-R is respectively such as sequence table SEQ ID
Shown in NO:89 and SEQ ID NO:90；
The site InDel46: forward primer Chr05-NP9-F and reverse primer Chr05-NP9-R is respectively such as sequence table SEQ ID
Shown in NO:91 and SEQ ID NO:92；
The site InDel47: forward primer Chr05-NP10-F and reverse primer Chr05-NP10-R is respectively such as sequence table SEQ ID
Shown in NO:93 and SEQ ID NO:94；
The site InDel48: forward primer Chr06-MP1-F and reverse primer Chr06-MP1-R is respectively such as sequence table SEQ ID
Shown in NO:95 and SEQ ID NO:96；
The site InDel49: forward primer Chr06-MP8-F and reverse primer Chr06-MP8-R is respectively such as sequence table SEQ ID
Shown in NO:97 and SEQ ID NO:98；
The site InDel50: forward primer Chr06-MP12-F and reverse primer Chr06-MP12-R is respectively such as sequence table SEQ ID
Shown in NO:99 and SEQ ID NO:100；
The site InDel51: forward primer Chr06-NP2-F and reverse primer Chr06-NP2-R is respectively such as sequence table SEQ ID
Shown in NO:101 and SEQ ID NO:102；
The site InDel52: forward primer Chr06-NP7-F and reverse primer Chr06-NP7-R is respectively such as sequence table SEQ ID
Shown in NO:103 and SEQ ID NO:104；
The site InDel53: forward primer Chr07-NP1-F and reverse primer Chr07-NP1-R is respectively such as sequence table SEQ ID
Shown in NO:105 and SEQ ID NO:106；
The site InDel54: forward primer Chr07-NP3-F and reverse primer Chr07-NP3-R is respectively such as sequence table SEQ ID
Shown in NO:107 and SEQ ID NO:108；
The site InDel55: forward primer Chr07-NP6-F and reverse primer Chr07-NP6-R is respectively such as sequence table SEQ ID
Shown in NO:109 and SEQ ID NO:110；
The site InDel56: forward primer Chr07-MP8-F and reverse primer Chr07-MP8-R is respectively such as sequence table SEQ ID
Shown in NO:111 and SEQ ID NO:112；
The site InDel57: forward primer Chr08-MP1-F and reverse primer Chr08-MP1-R is respectively such as sequence table SEQ ID
Shown in NO:113 and SEQ ID NO:114；
The site InDel58: forward primer Chr08-MP12-F and reverse primer Chr08-MP12-R is respectively such as sequence table SEQ ID
Shown in NO:115 and SEQ ID NO:116；
The site InDel59: forward primer Chr08-AP2-F and reverse primer Chr08-AP2-R is respectively such as sequence table SEQ ID
Shown in NO:117 and SEQ ID NO:118；
The site InDel60: forward primer Chr08-AP4-F and reverse primer Chr08-AP4-R is respectively such as sequence table SEQ ID
Shown in NO:119 and SEQ ID NO:120；
The site InDel61: forward primer Chr08-AP5-F and reverse primer Chr08-AP5-R is respectively such as sequence table SEQ ID
Shown in NO:121 and SEQ ID NO:122；
The site InDel62: forward primer Chr08-NP3-F and reverse primer Chr08-NP3-R is respectively such as sequence table SEQ ID
Shown in NO:123 and SEQ ID NO:124；
The site InDel63: forward primer Chr09-MP1-F and reverse primer Chr09-MP1-R is respectively such as sequence table SEQ ID
Shown in NO:125 and SEQ ID NO:126；
The site InDel64: forward primer Chr09-YM1-F and reverse primer Chr09-YM1-R is respectively such as sequence table SEQ ID
Shown in NO:127 and SEQ ID NO:128；
The site InDel65: forward primer Chr09-YM2-F and reverse primer Chr09-YM2-R is respectively such as sequence table SEQ ID
Shown in NO:129 and SEQ ID NO:130；
The site nDel66: forward primer Chr09-YM5-F and reverse primer Chr09-YM5-R is respectively such as sequence table SEQ ID NO:
Shown in 131 and SEQ ID NO:132；
The site nDel67: forward primer Chr09-YM10-F and reverse primer Chr09-YM10-R is respectively such as sequence table SEQ ID
Shown in NO:133 and SEQ ID NO:134；
The site nDel68: forward primer Chr09-YM11-F and reverse primer Chr09-YM11-R is respectively such as sequence table SEQ ID
Shown in NO:135 and SEQ ID NO:136；
The site InDel69: forward primer Chr09-MP4-F and reverse primer Chr09-MP4-R is respectively such as sequence table SEQ ID
Shown in NO:137 and SEQ ID NO:138；
The site InDel70: forward primer Chr09-MP5-F and reverse primer Chr09-MP5-R is respectively such as sequence table SEQ ID
Shown in NO:139 and SEQ ID NO:140；
The site InDel71: forward primer Chr09-MP9-F and reverse primer Chr09-MP9-R is respectively such as sequence table SEQ ID
Shown in NO:141 and SEQ ID NO:142；
The site InDel72: forward primer Chr09-MP11-F and reverse primer Chr09-MP11-R is respectively such as sequence table SEQ ID
Shown in NO:143 and SEQ ID NO:144；
The site InDel73: forward primer Chr09-NP1-F and reverse primer Chr09-NP1-R is respectively such as sequence table SEQ ID
Shown in NO:145 and SEQ ID NO:146；
The site InDel74: forward primer Chr09-NP2-F and reverse primer Chr09-NP2-R is respectively such as sequence table SEQ ID
Shown in NO:147 and SEQ ID NO:148；
The site InDel75: forward primer Chr09-NP4-F and reverse primer Chr09-NP4-R is respectively such as sequence table SEQ ID
Shown in NO:149 and SEQ ID NO:150；
The site InDel76: forward primer Chr09-NP5-F and reverse primer Chr09-NP5-R is respectively such as sequence table SEQ ID
Shown in NO:151 and SEQ ID NO:152；
The site InDel77: forward primer Chr09-NP9-F and reverse primer Chr09-NP9-R is respectively such as sequence table SEQ ID
Shown in NO:153 and SEQ ID NO:154；
The site InDel78: forward primer Chr10-MP2-F and reverse primer Chr10-MP2-R is respectively such as sequence table SEQ ID
Shown in NO:155 and SEQ ID NO:156；
The site InDel79: forward primer Chr10-MP5-F and reverse primer Chr10-MP5-R is respectively such as sequence table SEQ ID
Shown in NO:157 and SEQ ID NO:158；
The site InDel80: forward primer Chr10-MP7-F and reverse primer Chr10-MP7-R is respectively such as sequence table SEQ ID
Shown in NO:159 and SEQ ID NO:160；
The site InDel81: forward primer Chr10-MP8-F and reverse primer Chr10-MP8-R is respectively such as sequence table SEQ ID
Shown in NO:161 and SEQ ID NO:162；
The site InDel82: forward primer Chr10-MP11-F and reverse primer Chr10-MP11-R is respectively such as sequence table SEQ ID
Shown in NO:163 and SEQ ID NO:164；
The site InDel83: forward primer Chr10-NP4-F and reverse primer Chr10-NP4-R is respectively such as sequence table SEQ ID
Shown in NO:165 and SEQ ID NO:166；
The site InDel84: forward primer Chr10-NP6-F and reverse primer Chr10-NP6-R is respectively such as sequence table SEQ ID
Shown in NO:167 and SEQ ID NO:168；
The site InDel85: forward primer Chr10-NP7-F and reverse primer Chr10-NP7-R is respectively such as sequence table SEQ ID
Shown in NO:169 and SEQ ID NO:170；
The site InDel86: forward primer Chr10-NP9-F and reverse primer Chr10-NP9-R is respectively such as sequence table SEQ ID
Shown in NO:171 and SEQ ID NO:172；
The site InDel87: forward primer Chr10-NP11-F and reverse primer Chr10-NP11-R is respectively such as sequence table SEQ ID
Shown in NO:173 and SEQ ID NO:174.
2. application of the sorghum InDel molecular labeling described in claim 1 in the building of sorghum genetic linkage maps.
3. application of the sorghum InDel molecular labeling in Sorghum Germplasm analysis of genetic diversity described in claim 1.
4. application of the sorghum InDel molecular labeling in terms of sorghum hybrid/Purity described in claim 1.
5. sorghum InDel molecular labeling answering in terms of the positioning of sorghum Main Agronomic Characters related gene described in claim 1
With.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101821409A (en) * | 2007-08-29 | 2010-09-01 | 孟山都技术公司 | Methods and compositions for breeding for preferred traits |
| WO2016183005A1 (en) * | 2015-05-12 | 2016-11-17 | E I Du Pont De Nemours And Company | Methods and compositions to alter flowering time in sorghum |
-
2019
- 2019-04-09 CN CN201910282381.XA patent/CN109811087B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101821409A (en) * | 2007-08-29 | 2010-09-01 | 孟山都技术公司 | Methods and compositions for breeding for preferred traits |
| WO2016183005A1 (en) * | 2015-05-12 | 2016-11-17 | E I Du Pont De Nemours And Company | Methods and compositions to alter flowering time in sorghum |
Non-Patent Citations (2)
| Title |
|---|
| YUN SONG等: "Comparative Chloroplast Genomes of Sorghum Species: Sequence Divergence and Phylogenetic Relationships", 《BIOMED RESEARCH INTERNATIONAL》 * |
| 卢峰等: "甜高粱茎秆含糖量性状的QTL定位", 《分子植物育种》 * |
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