CN109811025A - A kind of extracting method of collagen - Google Patents
A kind of extracting method of collagen Download PDFInfo
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- CN109811025A CN109811025A CN201711174897.XA CN201711174897A CN109811025A CN 109811025 A CN109811025 A CN 109811025A CN 201711174897 A CN201711174897 A CN 201711174897A CN 109811025 A CN109811025 A CN 109811025A
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- collagen
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Abstract
The invention discloses a kind of extracting methods of collagen in collagen preparation field, comprising the following steps: (1), the stripping and slicing after over cleaning of ox heel string obtain fragment；(2), the fragment is added sodium chloride solution stirring and removes desalination solubility impurity；(3), the fragment for removing desalination solubility impurity is cleaned；(4), the acetone soln of the fragment after the cleaning impregnates removal lipid material.(5), the fragment after the removal lipid material air-dries after being cleaned；(6), it is described air-dry after fragment pepsin is added is impregnated in acetic acid and obtain extracting solution；Stripping and slicing is cleaned by ox heel string, it is air-dried after removing impurity and lipid material, collagen centrifugation object is obtained using centrifugation of saltouing after stripping and slicing pepsin after air-drying, collagen is obtained after dialysis, the extraction efficiency of collagen can be made to greatly improve in this way, quality is greatly improved, and the present invention can be used among the preparation of collagen.
Description
Technical field
The present invention relates to a kind of preparation method of chemicals, in particular to a kind of extracting method of collagen.
Background technique
Collagen is boiomacromolecule, the main component in animal connective tissue, and content is most in the mammalian body
Functional protein more, distribution is most wide, accounts for the 25%~30% of total protein, certain organisms are even as high as 80% or more.Livestock and poultry
Source animal tissue is the main path that people obtain natural collagen protein and its collagen peptide, but due to related domestic animals disease and certain
Religious belief limits use of the people to terrestrial mammalian collagen and its product, and it is raw gradually to turn to ocean now
It is developed in object.European Food Safety Authority (EFSA) is even infection is also not present in the collagen for having had proven to animal skeleton source
The possibility of rabid ox disease and other related diseases.Due to the difference of amino acid composition and the degree of cross linking etc., so that aquatic livestock is outstanding
It is its processing waste-skin, bone, there are many livestock collagens not had for collagen abundant contained in squama
The advantages of, in addition it is substantially better than the collagen of terrestrial animal in some respects from the collagen of marine animal, such as
With characteristics such as low antigenicity, hypoallergenics.Therefore aquatic collagen protein may gradually substitute terrestrial animal collagen.Collagen
Protein classes are more, and common type is I type, II type, III type, V type and Ⅺ type.Collagen is because having good bio-compatible
Property, biodegradable and bioactivity, therefore obtain in fields such as food, medicine, organizational project, cosmetics and widely answer
With.
Summary of the invention
The object of the present invention is to provide a kind of extracting methods of collagen, clean stripping and slicing by ox heel string, remove impurity
It is air-dried with after lipid material, collagen centrifugation object is obtained using centrifugation of saltouing after the stripping and slicing pepsin after air-drying, is passed through
Collagen is obtained after dialysis, the extraction efficiency of collagen can be made to greatly improve in this way, quality is greatly improved.
, ox to achieve the above object, the present invention provides a kind of extracting methods of collagen, comprising the following steps: (1)
Heel string stripping and slicing after over cleaning obtains fragment；(2), the fragment is added sodium chloride solution stirring and removes desalination solubility impurity；(3),
The fragment for removing desalination solubility impurity is cleaned；(4), the acetone soln of the fragment after the cleaning impregnates removal lipid
Matter；(5), the fragment after the removal lipid material air-dries after being cleaned；(6), it is described air-dry after fragment be added stomach cardia
Enzyme impregnates in acetic acid obtains extracting solution；(7), it is handled after the extracting solution filtering through oversalting, and glue is obtained using Li Xinfa
Former salting-out precipitate；(8), the collagen salting-out precipitate obtains collagen after dialysis.
Compared with prior art, the beneficial effects of the present invention are clean stripping and slicing by ox heel string, remove impurity and lipid
It is air-dried after substance, collagen centrifugation object is obtained using centrifugation of saltouing after the stripping and slicing pepsin after air-drying, after dialysis
Collagen is obtained, the extraction efficiency of collagen can be made to greatly improve in this way, quality is greatly improved, the present invention
It can be used among the preparation of collagen.
As a further improvement of the present invention, the fragment is not more than 5mm, can allow fragment preferably quilt in this way
Processing.
As a further improvement of the present invention, the concentration of the sodium chloride solution is 1mol/L, so that impurity is more preferable
Be removed.
As a further improvement of the present invention, the time of the stirring is 24 hours, is enough to thoroughly remove impurity in this way.
As a further improvement of the present invention, the time that the acetone soln impregnates is 12 hours, is enough in this way by lipid
Substance is removed.
As a further improvement of the present invention, it need to be placed in draught cupboard when the acetone soln impregnates, acetone is toxic, puts
Setting can be to avoid poisoning in draught cupboard.
As a further improvement of the present invention, the temperature for obtaining extracting solution is 25 DEG C, can make stomach cardia in this way
The activity of enzyme is best.
Detailed description of the invention
Fig. 1 is flow chart of the present invention.
Specific embodiment
The following further describes the present invention with reference to the drawings:
The extracting method of a kind of collagen as shown in Figure 1, comprising the following steps: (1), the stripping and slicing after over cleaning of ox heel string obtains
To fragment；(2), fragment is added sodium chloride solution stirring and removes desalination solubility impurity；(3), the fragment of desalination solubility impurity is gone to carry out
Cleaning；(4), the fragment after cleaning impregnates removal lipid material with acetone soln；(5), the fragment after removing lipid material carries out
It is air-dried after cleaning；(6), the fragment addition pepsin after air-drying impregnates in acetic acid obtains extracting solution；(7), extracting solution filters
It is handled by oversalting, and collagen salting-out precipitate is obtained using Li Xinfa；(8), collagen salting-out precipitate obtains collagen egg after dialysis
It is white；Fragment is not more than 5mm；The concentration of sodium chloride solution is 1mol/L；The time of stirring is 24 hours；Acetone soln impregnate when
Between be 12 hours；Acetone soln need to be placed in draught cupboard when impregnating；The temperature for obtaining extracting solution is 25 DEG C.
When work, ox heel string cleans stripping and slicing, and stripping and slicing size is not more than 5mm, and it is that 1mol/L sodium chloride solution stirs that concentration, which is added,
Stripping and slicing is cleaned up after mixing 24 hours, is impregnated with acetone soln, soaking process carries out in draught cupboard, washes after impregnating 12 hours
It is net to air-dry, pepsin is added and acetum is reacted to obtain extracting solution at 25 DEG C, extracting solution carries out after filtering
It saltouts processing, processing five obtains collagen salting-out precipitate by centrifugal process, and collagen salting-out precipitate obtains collagen after dialysis.
The present invention is not limited to the above embodiment, on the basis of the technical solution of the disclosure, those skilled in the art
According to disclosed technology contents, some replacements can be made to some of which technical characteristic by not needing creative labor
And deformation, these replacements and deformation are within the scope of the invention.
Claims (7)
1. a kind of extracting method of collagen, which comprises the following steps:
(1), ox heel string stripping and slicing after over cleaning obtains fragment；
(2), the fragment is added sodium chloride solution stirring and removes desalination solubility impurity；
(3), the fragment for removing desalination solubility impurity is cleaned；
(4), the acetone soln of the fragment after the cleaning impregnates removal lipid material；
(5), the fragment after the removal lipid material air-dries after being cleaned；
(6), it is described air-dry after fragment pepsin is added is impregnated in acetic acid and obtain extracting solution；
(7), it is handled after the extracting solution filtering through oversalting, and collagen salting-out precipitate is obtained using Li Xinfa；
(8), the collagen salting-out precipitate obtains collagen after dialysis.
2. a kind of extracting method of collagen according to claim 1, it is characterised in that: the fragment is not more than 5mm.
3. a kind of extracting method of collagen according to claim 1, it is characterised in that: the sodium chloride solution it is dense
Degree is 1mol/L.
4. a kind of extracting method of collagen according to claim 1, it is characterised in that: the time of the stirring is 24
Hour.
5. a kind of extracting method of collagen according to claim 1, it is characterised in that: what the acetone soln impregnated
Time is 12 hours.
6. a kind of extracting method of collagen according to claim 1, it is characterised in that: when the acetone soln impregnates
It need to be placed in draught cupboard.
7. a kind of extracting method of collagen according to claim 1, it is characterised in that: the temperature for obtaining extracting solution
Degree is 25 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711174897.XA CN109811025A (en) | 2017-11-22 | 2017-11-22 | A kind of extracting method of collagen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711174897.XA CN109811025A (en) | 2017-11-22 | 2017-11-22 | A kind of extracting method of collagen |
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| CN109811025A true CN109811025A (en) | 2019-05-28 |
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ID=66601264
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| CN201711174897.XA Pending CN109811025A (en) | 2017-11-22 | 2017-11-22 | A kind of extracting method of collagen |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112625122A (en) * | 2020-12-30 | 2021-04-09 | 福建纽伯尔生物科技有限公司 | Collagen fiber extraction method |
-
2017
- 2017-11-22 CN CN201711174897.XA patent/CN109811025A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112625122A (en) * | 2020-12-30 | 2021-04-09 | 福建纽伯尔生物科技有限公司 | Collagen fiber extraction method |
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Application publication date: 20190528 |